Lsi11G005090 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi11G005090
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionGDSL esterase/lipase
Locationchr11: 5408872 .. 5411143 (-)
RNA-Seq ExpressionLsi11G005090
SyntenyLsi11G005090
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTAAACCTCTTTTGCTTTCTTCCTTCTTCTTCTTCTTCTTCCCTCTTTTTGCTTACTGCTCACTGAACTGACCCACTTTCACCAACCATTCCTTCTTCCCCCACATTCCCATATATGCTTACAATCCCAATCCCACATTGCCCTATGCCACTTCCTCATCATTCTCTCTTCCACCGCCCATGGCCTCCGCCGCCGCCGTTTTCCTCTTCTTCCTCCTCTTTCTCGGCGTTCCGATGGCCAGATCCAGTCATTTCAACCGTCCCGCCGTTTTCAACTTCGGAGACTCCAATTCCGACACTGGCTGCCTTGTTAGTTCCGGGATTGAGACCATCAGCCCTCCCTATGGTCACCTCTTCTTCGGAAACCCTTCTGGAAGATACTGCGATGGCCGGCTCATCCTTGATTTCCTCTGTAATCTCCTGTTGTTTCCCTTGTTTTCTTTCGTTTTCGATAATTGAGATTTTTGTTTTATTTCGTGAAGTGACTGGGTACTGATGATTTTCTGCAGTGGATGCAATGGATTTGCCTTATTTGAATCCATATCTTGATTCCCTTGGCGCGCCGAATTTTCGAAAGGGGTGTAATTACGCGGCGGCGGCGTCGACTGTTCTTCCAGCGACTCCTACATCTTTTAGCCCTTTTTCCTTTGGGGTTCAAGTGAATCAGTTTATTCACTTCAAAGCTAGGGTTCTCGAGCTTCGATCTAAAGGTATGATTATCAATCATCTCGAGATTGAAGATTTTGAATGATCAAGGAAGGAACAATAACATCAACCAGTCTTCTTTTAGGTAAGAAACTTGATAAGTACCTACCAGAAGAAGATTACTTTCAGAAGGGGCTTTACATGTTTGATATTGGCCAGAATGACCTTGCTGCTGCATTTTACTCCAAAACTCTGGATCAAATTCTTGCTTCTATACCCACCATTTTAGCCGAGTTCGAGACTGGAATTCAGGTCTGATTCAATGGTTTCTTACTAAATTTTGACACAGTGATAGTTTAGTTGGGATCTCTTTGACCTCTTGTAGATAGAGTAGAGCATTTTTACAGCTTGGTATGGGATGTTTAGTGAGTAGAGCTTTATATAAAATTTTAAATTCAAAAGTTGACTGTTTCCACTCTAAATTTATTTGTTTTGTTATTAACTTTCTAAGTTTTGAAAAAAAAAAGAAGTTTTTATTAATTTGATTGAACTCAAATGTTCCTTTAAGAAATGGGACAACCATAATAAAGGAAAAATTATATGGAAAGAAGTTTTGATTTCAAAACCAGAAAACTAAAGCAAATGCAAAACTAAAATGAAGTAAATATCGAAATTAAAATGAGCTTTTCTTTAGATTTTTGATAGAAATATTAGAACATAAGAGAACAGGGTGAGGAAGAAAGTTCAAGTTCATTTTTTATAGGCCATATGAAATGATTTTATGGTTCTGAAGCCATTGCATTTGCTACATTTCAGAGACTGTACGACCAAGGGGCTAGGAATTTCTGGATTCACAACACTGGCCCTCTAGGATGCTTGGCTCAGAATGTTGCTAGATTCGGAACTGCCCCATCGAAGCTTGATGAACTTGGATGCGTCAGTTCACACAACCAAGCTGCCAAACTCTTCAATTCACAGCTTCATGCTCTCTTTAAAAAACTGCAGGAACAATATACAGATGGGAATGTCACATATGTCGATATCTATACTATAAAATCCAATCTCATTGCCAATTATTCGAGATTCGGTCAGCATCTTACAGTGTTTTTGGTCTCATTCACTGTTAATCATGCAAGGACATACCTGACTTTCTGCATTGGCACTTGCAGGTTTTGAACAACCTATTATGGCTTGTTGTGGCTACGGAGGTCCACCACTCAACTATGATAGTCGAATTGTCTGTGGGCAAACTAAGACATTGAATGGGACAGTGGTCACAGCGCAAGGGTGCGATGATAGCTCGGAGTACATCAATTGGGATGGAATTCATTATACAGAGGCTGCAAATCAGTATGTGTCATCACAAATACTCACTGGAAAATATTGTGATCCACCCTTCTCAGACAAAATGCCTTTCCTTCTCAAACTCAAGTTCTAGGCTTCCTTGATTATATACATTTCATCTCCATCATATCTTTACTATCTTAATATGTCTAAATGTAAGCATTCATTTGGGAATTGAAATAAAGATCTGACCAACAATGCATTTATGTTTAGACACTGTATATTGTGTAGCTAAATAAAAAAATTACAAGAATTTTAAACAATTCTTGGAACTTCATATAG

mRNA sequence

CTTTAAACCTCTTTTGCTTTCTTCCTTCTTCTTCTTCTTCTTCCCTCTTTTTGCTTACTGCTCACTGAACTGACCCACTTTCACCAACCATTCCTTCTTCCCCCACATTCCCATATATGCTTACAATCCCAATCCCACATTGCCCTATGCCACTTCCTCATCATTCTCTCTTCCACCGCCCATGGCCTCCGCCGCCGCCGTTTTCCTCTTCTTCCTCCTCTTTCTCGGCGTTCCGATGGCCAGATCCAGTCATTTCAACCGTCCCGCCGTTTTCAACTTCGGAGACTCCAATTCCGACACTGGCTGCCTTGTTAGTTCCGGGATTGAGACCATCAGCCCTCCCTATGGTCACCTCTTCTTCGGAAACCCTTCTGGAAGATACTGCGATGGCCGGCTCATCCTTGATTTCCTCTTGGATGCAATGGATTTGCCTTATTTGAATCCATATCTTGATTCCCTTGGCGCGCCGAATTTTCGAAAGGGGTGTAATTACGCGGCGGCGGCGTCGACTGTTCTTCCAGCGACTCCTACATCTTTTAGCCCTTTTTCCTTTGGGGTTCAAGTGAATCAGTTTATTCACTTCAAAGCTAGGGTTCTCGAGCTTCGATCTAAAGGAACAATAACATCAACCAGTCTTCTTTTAGGTAAGAAACTTGATAAGTACCTACCAGAAGAAGATTACTTTCAGAAGGGGCTTTACATGTTTGATATTGGCCAGAATGACCTTGCTGCTGCATTTTACTCCAAAACTCTGGATCAAATTCTTGCTTCTATACCCACCATTTTAGCCGAGTTCGAGACTGGAATTCAGAGTAGAGCATTTTTACAGCTTGGTATGGGATGTTTAAGACTGTACGACCAAGGGGCTAGGAATTTCTGGATTCACAACACTGGCCCTCTAGGATGCTTGGCTCAGAATGTTGCTAGATTCGGAACTGCCCCATCGAAGCTTGATGAACTTGGATGCGTCAGTTCACACAACCAAGCTGCCAAACTCTTCAATTCACAGCTTCATGCTCTCTTTAAAAAACTGCAGGAACAATATACAGATGGGAATGTCACATATGTCGATATCTATACTATAAAATCCAATCTCATTGCCAATTATTCGAGATTCGGTTTTGAACAACCTATTATGGCTTGTTGTGGCTACGGAGGTCCACCACTCAACTATGATAGTCGAATTGTCTGTGGGCAAACTAAGACATTGAATGGGACAGTGGTCACAGCGCAAGGGTGCGATGATAGCTCGGAGTACATCAATTGGGATGGAATTCATTATACAGAGGCTGCAAATCAGTATGTGTCATCACAAATACTCACTGGAAAATATTGTGATCCACCCTTCTCAGACAAAATGCCTTTCCTTCTCAAACTCAAGTTCTAGGCTTCCTTGATTATATACATTTCATCTCCATCATATCTTTACTATCTTAATATGTCTAAATGTAAGCATTCATTTGGGAATTGAAATAAAGATCTGACCAACAATGCATTTATGTTTAGACACTGTATATTGTGTAGCTAAATAAAAAAATTACAAGAATTTTAAACAATTCTTGGAACTTCATATAG

Coding sequence (CDS)

ATGGCCTCCGCCGCCGCCGTTTTCCTCTTCTTCCTCCTCTTTCTCGGCGTTCCGATGGCCAGATCCAGTCATTTCAACCGTCCCGCCGTTTTCAACTTCGGAGACTCCAATTCCGACACTGGCTGCCTTGTTAGTTCCGGGATTGAGACCATCAGCCCTCCCTATGGTCACCTCTTCTTCGGAAACCCTTCTGGAAGATACTGCGATGGCCGGCTCATCCTTGATTTCCTCTTGGATGCAATGGATTTGCCTTATTTGAATCCATATCTTGATTCCCTTGGCGCGCCGAATTTTCGAAAGGGGTGTAATTACGCGGCGGCGGCGTCGACTGTTCTTCCAGCGACTCCTACATCTTTTAGCCCTTTTTCCTTTGGGGTTCAAGTGAATCAGTTTATTCACTTCAAAGCTAGGGTTCTCGAGCTTCGATCTAAAGGAACAATAACATCAACCAGTCTTCTTTTAGGTAAGAAACTTGATAAGTACCTACCAGAAGAAGATTACTTTCAGAAGGGGCTTTACATGTTTGATATTGGCCAGAATGACCTTGCTGCTGCATTTTACTCCAAAACTCTGGATCAAATTCTTGCTTCTATACCCACCATTTTAGCCGAGTTCGAGACTGGAATTCAGAGTAGAGCATTTTTACAGCTTGGTATGGGATGTTTAAGACTGTACGACCAAGGGGCTAGGAATTTCTGGATTCACAACACTGGCCCTCTAGGATGCTTGGCTCAGAATGTTGCTAGATTCGGAACTGCCCCATCGAAGCTTGATGAACTTGGATGCGTCAGTTCACACAACCAAGCTGCCAAACTCTTCAATTCACAGCTTCATGCTCTCTTTAAAAAACTGCAGGAACAATATACAGATGGGAATGTCACATATGTCGATATCTATACTATAAAATCCAATCTCATTGCCAATTATTCGAGATTCGGTTTTGAACAACCTATTATGGCTTGTTGTGGCTACGGAGGTCCACCACTCAACTATGATAGTCGAATTGTCTGTGGGCAAACTAAGACATTGAATGGGACAGTGGTCACAGCGCAAGGGTGCGATGATAGCTCGGAGTACATCAATTGGGATGGAATTCATTATACAGAGGCTGCAAATCAGTATGTGTCATCACAAATACTCACTGGAAAATATTGTGATCCACCCTTCTCAGACAAAATGCCTTTCCTTCTCAAACTCAAGTTCTAG

Protein sequence

MASAAAVFLFFLLFLGVPMARSSHFNRPAVFNFGDSNSDTGCLVSSGIETISPPYGHLFFGNPSGRYCDGRLILDFLLDAMDLPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFSPFSFGVQVNQFIHFKARVLELRSKGTITSTSLLLGKKLDKYLPEEDYFQKGLYMFDIGQNDLAAAFYSKTLDQILASIPTILAEFETGIQSRAFLQLGMGCLRLYDQGARNFWIHNTGPLGCLAQNVARFGTAPSKLDELGCVSSHNQAAKLFNSQLHALFKKLQEQYTDGNVTYVDIYTIKSNLIANYSRFGFEQPIMACCGYGGPPLNYDSRIVCGQTKTLNGTVVTAQGCDDSSEYINWDGIHYTEAANQYVSSQILTGKYCDPPFSDKMPFLLKLKF
Homology
BLAST of Lsi11G005090 vs. ExPASy Swiss-Prot
Match: Q3ECP6 (GDSL esterase/lipase At1g54790 OS=Arabidopsis thaliana OX=3702 GN=At1g54790 PE=2 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 4.3e-148
Identity = 263/421 (62.47%), Postives = 306/421 (72.68%), Query Frame = 0

Query: 7   VFLFFLLFLGVPMARSSHFNRPAVFNFGDSNSDTGCLVSSGIETISPPYGHLFFGNPSGR 66
           V LFF+  L +  + S  FN P+ FNFGDSNSDTG LV+     +  P G   F   S R
Sbjct: 11  VILFFISSLQI--SNSIDFNYPSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKTSSQR 70

Query: 67  YCDGRLILDFLLDAMDLPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFSPFSFGV 126
           +CDGRL++DFL+D MDLP+LNPYLDSLG PNF+KGCN+AAA ST+LPA PTS SPFSF +
Sbjct: 71  FCDGRLVIDFLMDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDL 130

Query: 127 QVNQFIHFKARVLELRSKGTITSTSLLLGKKLDKYLPEEDYFQKGLYMFDIGQNDLAAAF 186
           Q++QFI FK+R +EL SK          G+K +KYLP  DY+ KGLYM DIGQND+A AF
Sbjct: 131 QISQFIRFKSRAIELLSK---------TGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAF 190

Query: 187 YSKTLDQILASIPTILAEFETGIQSRAFLQLGMGCLRLYDQGARNFWIHNTGPLGCLAQN 246
           YSKTLDQ+LASIP+IL  FE G++            RLY++G RN WIHNTGPLGCLAQN
Sbjct: 191 YSKTLDQVLASIPSILETFEAGLK------------RLYEEGGRNIWIHNTGPLGCLAQN 250

Query: 247 VARFGTAPSKLDELGCVSSHNQAAKLFNSQLHALFKKLQEQYTDGNVTYVDIYTIKSNLI 306
           +A+FGT  +KLDE GCVSSHNQAAKLFN QLHA+  K Q QY D NVTYVDI++IKSNLI
Sbjct: 251 IAKFGTDSTKLDEFGCVSSHNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLI 310

Query: 307 ANYSRF--------------------------GFEQPIMACCGYGGPPLNYDSRIVCGQT 366
           ANYSRF                          GFE+P+MACCG GG PLNYDSRI CGQT
Sbjct: 311 ANYSRFGKHFTKPLIDLNHLENVGYNKILNVLGFEKPLMACCGVGGAPLNYDSRITCGQT 370

Query: 367 KTLNGTVVTAQGCDDSSEYINWDGIHYTEAANQYVSSQILTGKYCDPPFSDKMPFLLKLK 402
           K L+G  VTA+ C+DSSEYINWDGIHYTEAAN++VSSQILTGKY DPPFSD+MPF L LK
Sbjct: 371 KVLDGISVTAKACNDSSEYINWDGIHYTEAANEFVSSQILTGKYSDPPFSDQMPFFLTLK 408

BLAST of Lsi11G005090 vs. ExPASy Swiss-Prot
Match: Q9MAA1 (GDSL esterase/lipase At3g05180 OS=Arabidopsis thaliana OX=3702 GN=At3g05180 PE=2 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 3.1e-122
Identity = 222/387 (57.36%), Postives = 281/387 (72.61%), Query Frame = 0

Query: 9   LFFLLFLGV-----PMARSSHFNR--PAVFNFGDSNSDTGCLVSSGIETI-SPPYGHLFF 68
           L  LLF+ +     P+A S   +   PAVFNFGDSNSDTG L SSG+  +  P Y   FF
Sbjct: 9   LRLLLFVAISHTLSPLAGSFRISNDFPAVFNFGDSNSDTGEL-SSGLGFLPQPSYEITFF 68

Query: 69  GNP-SGRYCDGRLILDFLLDAMDLPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSF 128
            +P SGR+C+GRLI+DFL++A+D PYL PYLDS+    +R+GCN+AAAAST+  A   S+
Sbjct: 69  RSPTSGRFCNGRLIVDFLMEAIDRPYLRPYLDSISRQTYRRGCNFAAAASTIQKANAASY 128

Query: 129 SPFSFGVQVNQFIHFKARVLELRSKGTITSTSLLLGKKLDKYLPEEDYFQKGLYMFDIGQ 188
           SPF FGVQV+QFI FK++VL+L          +   ++L +YLP E +F  GLYMFDIGQ
Sbjct: 129 SPFGFGVQVSQFITFKSKVLQL----------IQQDEELQRYLPSEYFFSNGLYMFDIGQ 188

Query: 189 NDLAAAFYSKTLDQILASIPTILAEFETGIQSRAFLQLGMGCLRLYDQGARNFWIHNTGP 248
           ND+A AFY+KT+DQ+LA +P IL  F+ GI+            RLY +GARN+WIHNTGP
Sbjct: 189 NDIAGAFYTKTVDQVLALVPIILDIFQDGIK------------RLYAEGARNYWIHNTGP 248

Query: 249 LGCLAQNVARFGTAPSKLDELGCVSSHNQAAKLFNSQLHALFKKLQEQYTDGNVTYVDIY 308
           LGCLAQ V+ FG   SKLDE GCVS HNQAAKLFN QLH LFKKL +QY +   TYVDI+
Sbjct: 249 LGCLAQVVSIFGEDKSKLDEFGCVSDHNQAAKLFNLQLHGLFKKLPQQYPNSRFTYVDIF 308

Query: 309 TIKSNLIANYSRFGFEQPIMACCGYGGPPLNYDSRIVCGQTKTLNGTVVTAQGCDDSSEY 368
           +IKS+LI N+S++GF+  IM CCG GGPPLNYD ++ CG+T   NGT++TA+ C DSS+Y
Sbjct: 309 SIKSDLILNHSKYGFDHSIMVCCGTGGPPLNYDDQVGCGKTARSNGTIITAKPCYDSSKY 368

Query: 369 INWDGIHYTEAANQYVSSQILTGKYCD 387
           +NWDGIHYTEAAN++V+  ILTGKY +
Sbjct: 369 VNWDGIHYTEAANRFVALHILTGKYSE 372

BLAST of Lsi11G005090 vs. ExPASy Swiss-Prot
Match: Q9FXB6 (GDSL esterase/lipase LIP-4 OS=Arabidopsis thaliana OX=3702 GN=LIP4 PE=2 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.6e-81
Identity = 167/378 (44.18%), Postives = 223/378 (58.99%), Query Frame = 0

Query: 12  LLFLGVPMARSSHFNRPAVFNFGDSNSDTGCLVSSGIETISPPYGHLFFGNPSGRYCDGR 71
           LL L  P   +S   RP +FNFGDSNSDTG LV+     I  P G LFF   +GR  DGR
Sbjct: 23  LLILRQPSRAASCTARPVIFNFGDSNSDTGGLVAGLGYPIGFPNGRLFFRRSTGRLSDGR 82

Query: 72  LILDFLLDAMDLPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFSPFSFGVQVNQF 131
           L++DFL  +++   L PYLDSLG   F+ G N+A A S  LP       PFS  +QV QF
Sbjct: 83  LLIDFLCQSLNTSLLRPYLDSLGRTRFQNGANFAIAGSPTLPKN----VPFSLNIQVKQF 142

Query: 132 IHFKARVLELRSKGTITSTSLLLGKKLDKYLPEEDYFQKGLYMFDIGQNDLAAAF-YSKT 191
            HFK+R LEL S     S++ L G  +       + F+  LYM DIGQND+A +F    +
Sbjct: 143 SHFKSRSLELAS-----SSNSLKGMFI-----SNNGFKNALYMIDIGQNDIARSFARGNS 202

Query: 192 LDQILASIPTILAEFETGIQSRAFLQLGMGCLRLYDQGARNFWIHNTGPLGCLAQNVARF 251
             Q +  IP I+ E ++ I+            RLYD+G R FWIHNTGPLGCL Q ++  
Sbjct: 203 YSQTVKLIPQIITEIKSSIK------------RLYDEGGRRFWIHNTGPLGCLPQKLSM- 262

Query: 252 GTAPSKLDELGCVSSHNQAAKLFNSQLHALFKKLQEQYTDGNVTYVDIYTIKSNLIANYS 311
                 LD+ GC+ S+N AA LFN  L  + ++L+ +  D  + Y+DIY IK +LIAN +
Sbjct: 263 -VKSKDLDQHGCLVSYNSAATLFNQGLDHMCEELRTELRDATIIYIDIYAIKYSLIANSN 322

Query: 312 RFGFEQPIMACCGYGGPPLNYDSRIVCGQTKTLNGTVVTAQGCDDSSEYINWDGIHYTEA 371
           ++GF+ P+MACCGYGG P NY+ +I CG      G+ V    C++ S +I+WDGIHYTE 
Sbjct: 323 QYGFKSPLMACCGYGGTPYNYNVKITCGH----KGSNV----CEEGSRFISWDGIHYTET 364

Query: 372 ANQYVSSQILTGKYCDPP 389
           AN  V+ ++L+  Y  PP
Sbjct: 383 ANAIVAMKVLSMHYSKPP 364

BLAST of Lsi11G005090 vs. ExPASy Swiss-Prot
Match: O80522 (GDSL esterase/lipase At1g09390 OS=Arabidopsis thaliana OX=3702 GN=At1g09390 PE=2 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 6.0e-81
Identity = 157/363 (43.25%), Postives = 223/363 (61.43%), Query Frame = 0

Query: 28  PAVFNFGDSNSDTGCLVSSGIETISPPYGHLFFGNPSGRYCDGRLILDFLLDAMDLPYLN 87
           P +FNFGDSNSDTG LV+    +I  P G  FF   +GR  DGRL++DFL  +++   LN
Sbjct: 36  PVIFNFGDSNSDTGGLVAGLGYSIGLPNGRSFFQRSTGRLSDGRLVIDFLCQSLNTSLLN 95

Query: 88  PYLDSLGAPNFRKGCNYAAAASTVLPATPTSFSPFSFGVQVNQFIHFKARVLELRSKGTI 147
           PYLDSL    F+ G N+A   S+ LP     + PF+  +Q+ QF+HFK+R LEL S    
Sbjct: 96  PYLDSLVGSKFQNGANFAIVGSSTLP----RYVPFALNIQLMQFLHFKSRALELAS---- 155

Query: 148 TSTSLLLGKKLDKYLPEEDYFQKGLYMFDIGQNDLAAAFYSKTL--DQILASIPTILAEF 207
                 +   L + +  E  F+  LYM DIGQND+A +F SK L   +++  IP +++E 
Sbjct: 156 ------ISDPLKEMMIGESGFRNALYMIDIGQNDIADSF-SKGLSYSRVVKLIPNVISEI 215

Query: 208 ETGIQSRAFLQLGMGCLRLYDQGARNFWIHNTGPLGCLAQNVARFGTAPSKLDELGCVSS 267
           ++ I+             LYD+G R FW+HNTGPLGCL Q ++   +     D+ GC+++
Sbjct: 216 KSAIKI------------LYDEGGRKFWVHNTGPLGCLPQKLSMVHS--KGFDKHGCLAT 275

Query: 268 HNQAAKLFNSQLHALFKKLQEQYTDGNVTYVDIYTIKSNLIANYSRFGFEQPIMACCGYG 327
           +N AAKLFN  L  + + L+ +  + N+ YVDIY IK +LIAN + +GFE+P+MACCGYG
Sbjct: 276 YNAAAKLFNEGLDHMCRDLRTELKEANIVYVDIYAIKYDLIANSNNYGFEKPLMACCGYG 335

Query: 328 GPPLNYDSRIVCGQTKTLNGTVVTAQGCDDSSEYINWDGIHYTEAANQYVSSQILTGKYC 387
           GPP NY+  I CG     NG    ++ CD+ S +I+WDGIHYTE AN  V+ ++L+ ++ 
Sbjct: 336 GPPYNYNVNITCG-----NG---GSKSCDEGSRFISWDGIHYTETANAIVAMKVLSMQHS 361

Query: 388 DPP 389
            PP
Sbjct: 396 TPP 361

BLAST of Lsi11G005090 vs. ExPASy Swiss-Prot
Match: Q9FXE5 (Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.1e-78
Identity = 155/384 (40.36%), Postives = 224/384 (58.33%), Query Frame = 0

Query: 5   AAVFLFFLLFLGVPMARSSHFNRPAVFNFGDSNSDTGCLVSSGIETISPPYGHLFFGNPS 64
           +++F   LL    P   +   + PA+FNFGDSNSDTG L S+      PP+G  FFG+P+
Sbjct: 6   SSLFALSLLSSLSPSTHAHQCHFPAIFNFGDSNSDTGGL-SAAFGQAGPPHGSSFFGSPA 65

Query: 65  GRYCDGRLILDFLLDAMDLPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPT----SFS 124
           GRYCDGRL++DF+ +++ LPYL+ +LDS+G+ NF  G N+A A S +     T     FS
Sbjct: 66  GRYCDGRLVIDFIAESLGLPYLSAFLDSVGS-NFSHGANFATAGSPIRALNSTLRQSGFS 125

Query: 125 PFSFGVQVNQFIHFKARVLELRSKGTITSTSLLLGKKLDKYLPEEDYFQKGLYMFDIGQN 184
           PFS  VQ  QF +F  R   +RS+G +  T           LPE D F K LY FDIGQN
Sbjct: 126 PFSLDVQFVQFYNFHNRSQTVRSRGGVYKT----------MLPESDSFSKALYTFDIGQN 185

Query: 185 DLAAAFY-SKTLDQILASIPTILAEFETGIQSRAFLQLGMGCLRLYDQGARNFWIHNTGP 244
           DL A ++ +KT++Q+   +P I+++F   I++            +Y QG R FWIHNTGP
Sbjct: 186 DLTAGYFANKTVEQVETEVPEIISQFMNAIKN------------IYGQGGRYFWIHNTGP 245

Query: 245 LGCLAQNVARFGTAPSKLDELGCVSSHNQAAKLFNSQLHALFKKLQEQYTDGNVTYVDIY 304
           +GCLA  + RF    S  D  GCVS  N  A+ FN  L     +L+   ++  +TYVD+Y
Sbjct: 246 IGCLAYVIERFPNKASDFDSHGCVSPLNHLAQQFNHALKQAVIELRSSLSEAAITYVDVY 305

Query: 305 TIKSNLIANYSRFGFEQPIMACCGYGGPPLNYDSRIVCGQTKTLNG-TVVTAQGCDDSSE 364
           ++K  L  +    GF+  +++CCG+GG   NY+  I CG  K + G  V   + CD+  +
Sbjct: 306 SLKHELFVHAQGHGFKGSLVSCCGHGG-KYNYNKGIGCGMKKIVKGKEVYIGKPCDEPDK 364

Query: 365 YINWDGIHYTEAANQYVSSQILTG 383
            + WDG+H+T+AAN+++  +I  G
Sbjct: 366 AVVWDGVHFTQAANKFIFDKIAPG 364

BLAST of Lsi11G005090 vs. ExPASy TrEMBL
Match: A0A0A0LIJ5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G020920 PE=3 SV=1)

HSP 1 Score: 718.8 bits (1854), Expect = 1.3e-203
Identity = 352/401 (87.78%), Postives = 363/401 (90.52%), Query Frame = 0

Query: 1   MASAAAVFLFFLLFLGVPMARSSHFNRPAVFNFGDSNSDTGCLVSSGIETISPPYGHLFF 60
           MAS AA FLFFLLF G P ARSSHFNRPAVFNFGDSNSDTGCLVSSGIE I PPYGHLFF
Sbjct: 1   MASPAASFLFFLLFFGAPTARSSHFNRPAVFNFGDSNSDTGCLVSSGIEAIGPPYGHLFF 60

Query: 61  GNPSGRYCDGRLILDFLLDAMDLPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFS 120
           GNPSGRYCDGRLILDFLLDAMD+PYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFS
Sbjct: 61  GNPSGRYCDGRLILDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFS 120

Query: 121 PFSFGVQVNQFIHFKARVLELRSKGTITSTSLLLGKKLDKYLPEEDYFQKGLYMFDIGQN 180
           PFSFGVQVNQFIHFKARVLELRSK          GKKLDKYLP+EDYF+KGLYMFDIGQN
Sbjct: 121 PFSFGVQVNQFIHFKARVLELRSK----------GKKLDKYLPDEDYFEKGLYMFDIGQN 180

Query: 181 DLAAAFYSKTLDQILASIPTILAEFETGIQSRAFLQLGMGCLRLYDQGARNFWIHNTGPL 240
           DLA AFYSKTLDQILASIPTILA FETG+Q            +LYDQGARNFWIHNTGPL
Sbjct: 181 DLAIAFYSKTLDQILASIPTILAVFETGLQ------------KLYDQGARNFWIHNTGPL 240

Query: 241 GCLAQNVARFGTAPSKLDELGCVSSHNQAAKLFNSQLHALFKKLQEQYTDGNVTYVDIYT 300
           GCLAQNVARFGT PS LDELGCVSSHNQAAKLFN QLHAL K+LQE+YTD NVTYVDIYT
Sbjct: 241 GCLAQNVARFGTDPSNLDELGCVSSHNQAAKLFNLQLHALCKELQEEYTDTNVTYVDIYT 300

Query: 301 IKSNLIANYSRFGFEQPIMACCGYGGPPLNYDSRIVCGQTKTLNGTVVTAQGCDDSSEYI 360
           IKSNLIANYSRFGFEQPIMACCGYGGPPLNYDSRI+CGQTK LNGTVVTA+GCDDSSEYI
Sbjct: 301 IKSNLIANYSRFGFEQPIMACCGYGGPPLNYDSRIICGQTKILNGTVVTAKGCDDSSEYI 360

Query: 361 NWDGIHYTEAANQYVSSQILTGKYCDPPFSDKMPFLLKLKF 402
           NWDGIHY+EAAN+YVSSQILTGKY DPPFSDKMPFLLKLKF
Sbjct: 361 NWDGIHYSEAANKYVSSQILTGKYSDPPFSDKMPFLLKLKF 379

BLAST of Lsi11G005090 vs. ExPASy TrEMBL
Match: A0A1S3C1D3 (GDSL esterase/lipase At1g54790 OS=Cucumis melo OX=3656 GN=LOC103495359 PE=3 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 1.9e-202
Identity = 348/398 (87.44%), Postives = 362/398 (90.95%), Query Frame = 0

Query: 4   AAAVFLFFLLFLGVPMARSSHFNRPAVFNFGDSNSDTGCLVSSGIETISPPYGHLFFGNP 63
           A+AVFLFFLLF G+PMARSS FNRPAVFNFGDSNSDTGCLVSSGIETI PPYGHLFFGNP
Sbjct: 78  ASAVFLFFLLFFGLPMARSSPFNRPAVFNFGDSNSDTGCLVSSGIETIGPPYGHLFFGNP 137

Query: 64  SGRYCDGRLILDFLLDAMDLPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFSPFS 123
           SGRYCDGRLILDFLLDAMD+PYLNPYLDS+GAPNFRKGCNYAAAAST+LPATPTSFSPFS
Sbjct: 138 SGRYCDGRLILDFLLDAMDMPYLNPYLDSIGAPNFRKGCNYAAAASTILPATPTSFSPFS 197

Query: 124 FGVQVNQFIHFKARVLELRSKGTITSTSLLLGKKLDKYLPEEDYFQKGLYMFDIGQNDLA 183
           FGVQVNQFIHFKARVLELRSK           KKLDKYLP+EDYF+KGLYMFDIGQNDLA
Sbjct: 198 FGVQVNQFIHFKARVLELRSK----------DKKLDKYLPDEDYFEKGLYMFDIGQNDLA 257

Query: 184 AAFYSKTLDQILASIPTILAEFETGIQSRAFLQLGMGCLRLYDQGARNFWIHNTGPLGCL 243
            AFYSKTLDQILASIPTILA FETG+Q            +L+ QGARNFWIHNTGPLGCL
Sbjct: 258 IAFYSKTLDQILASIPTILAVFETGLQ------------KLHKQGARNFWIHNTGPLGCL 317

Query: 244 AQNVARFGTAPSKLDELGCVSSHNQAAKLFNSQLHALFKKLQEQYTDGNVTYVDIYTIKS 303
           AQNVARFGT PS LDELGCVSSHNQAAKLFN QLHAL KKLQ+QYTD NVTYVDIYTIKS
Sbjct: 318 AQNVARFGTDPSNLDELGCVSSHNQAAKLFNLQLHALCKKLQKQYTDANVTYVDIYTIKS 377

Query: 304 NLIANYSRFGFEQPIMACCGYGGPPLNYDSRIVCGQTKTLNGTVVTAQGCDDSSEYINWD 363
           NLIANYSR+GFEQPIMACCGYGGPPLNYDSRIVCGQTK LNGTVVTA+GCDDSSEYINWD
Sbjct: 378 NLIANYSRYGFEQPIMACCGYGGPPLNYDSRIVCGQTKILNGTVVTAKGCDDSSEYINWD 437

Query: 364 GIHYTEAANQYVSSQILTGKYCDPPFSDKMPFLLKLKF 402
           GIHYTE ANQYVSSQIL+GKYCDPPFSDKMPFLLKLKF
Sbjct: 438 GIHYTEVANQYVSSQILSGKYCDPPFSDKMPFLLKLKF 453

BLAST of Lsi11G005090 vs. ExPASy TrEMBL
Match: A0A5A7V7X2 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold329G001970 PE=3 SV=1)

HSP 1 Score: 711.4 bits (1835), Expect = 2.1e-201
Identity = 346/398 (86.93%), Postives = 361/398 (90.70%), Query Frame = 0

Query: 4   AAAVFLFFLLFLGVPMARSSHFNRPAVFNFGDSNSDTGCLVSSGIETISPPYGHLFFGNP 63
           A+A+FLFFLLF G+PMARSS FNRPAVFNFGDSNSDTGCLVSSGIETI PPYGHLFFGNP
Sbjct: 79  ASALFLFFLLFFGLPMARSSPFNRPAVFNFGDSNSDTGCLVSSGIETIGPPYGHLFFGNP 138

Query: 64  SGRYCDGRLILDFLLDAMDLPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFSPFS 123
           SGRYCDGRLILDFLLDAMD+PYLNPYLDS+GAPN RKGCNYAAAAST+LPATPTSFSPFS
Sbjct: 139 SGRYCDGRLILDFLLDAMDMPYLNPYLDSIGAPNLRKGCNYAAAASTILPATPTSFSPFS 198

Query: 124 FGVQVNQFIHFKARVLELRSKGTITSTSLLLGKKLDKYLPEEDYFQKGLYMFDIGQNDLA 183
           FGVQVNQFIHFKARVLELRSK           KKLDKYLP+EDYF+KGLYMFDIGQNDLA
Sbjct: 199 FGVQVNQFIHFKARVLELRSK----------DKKLDKYLPDEDYFEKGLYMFDIGQNDLA 258

Query: 184 AAFYSKTLDQILASIPTILAEFETGIQSRAFLQLGMGCLRLYDQGARNFWIHNTGPLGCL 243
            AFYSKTLDQILASIPTILA FETG+Q            +L+ QGARNFWIHNTGPLGCL
Sbjct: 259 IAFYSKTLDQILASIPTILAVFETGLQ------------KLHKQGARNFWIHNTGPLGCL 318

Query: 244 AQNVARFGTAPSKLDELGCVSSHNQAAKLFNSQLHALFKKLQEQYTDGNVTYVDIYTIKS 303
           AQNVARFGT PS LDELGCVSSHNQAAKLFN QLHAL KKLQ+QYTD NVTYVDIYTIKS
Sbjct: 319 AQNVARFGTDPSNLDELGCVSSHNQAAKLFNLQLHALCKKLQKQYTDANVTYVDIYTIKS 378

Query: 304 NLIANYSRFGFEQPIMACCGYGGPPLNYDSRIVCGQTKTLNGTVVTAQGCDDSSEYINWD 363
           NLIANYSR+GFEQPIMACCGYGGPPLNYDSRIVCGQTK LNGTVVTA+GCDDSSEYINWD
Sbjct: 379 NLIANYSRYGFEQPIMACCGYGGPPLNYDSRIVCGQTKILNGTVVTAKGCDDSSEYINWD 438

Query: 364 GIHYTEAANQYVSSQILTGKYCDPPFSDKMPFLLKLKF 402
           GIHYTE ANQYVSSQIL+GKYCDPPFSDKMPFLLKLKF
Sbjct: 439 GIHYTEVANQYVSSQILSGKYCDPPFSDKMPFLLKLKF 454

BLAST of Lsi11G005090 vs. ExPASy TrEMBL
Match: A0A5D3D471 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00640 PE=3 SV=1)

HSP 1 Score: 711.4 bits (1835), Expect = 2.1e-201
Identity = 346/398 (86.93%), Postives = 361/398 (90.70%), Query Frame = 0

Query: 4   AAAVFLFFLLFLGVPMARSSHFNRPAVFNFGDSNSDTGCLVSSGIETISPPYGHLFFGNP 63
           A+A+FLFFLLF G+PMARSS FNRPAVFNFGDSNSDTGCLVSSGIETI PPYGHLFFGNP
Sbjct: 2   ASALFLFFLLFFGLPMARSSPFNRPAVFNFGDSNSDTGCLVSSGIETIGPPYGHLFFGNP 61

Query: 64  SGRYCDGRLILDFLLDAMDLPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFSPFS 123
           SGRYCDGRLILDFLLDAMD+PYLNPYLDS+GAPN RKGCNYAAAAST+LPATPTSFSPFS
Sbjct: 62  SGRYCDGRLILDFLLDAMDMPYLNPYLDSIGAPNLRKGCNYAAAASTILPATPTSFSPFS 121

Query: 124 FGVQVNQFIHFKARVLELRSKGTITSTSLLLGKKLDKYLPEEDYFQKGLYMFDIGQNDLA 183
           FGVQVNQFIHFKARVLELRSK           KKLDKYLP+EDYF+KGLYMFDIGQNDLA
Sbjct: 122 FGVQVNQFIHFKARVLELRSK----------DKKLDKYLPDEDYFEKGLYMFDIGQNDLA 181

Query: 184 AAFYSKTLDQILASIPTILAEFETGIQSRAFLQLGMGCLRLYDQGARNFWIHNTGPLGCL 243
            AFYSKTLDQILASIPTILA FETG+Q            +L+ QGARNFWIHNTGPLGCL
Sbjct: 182 IAFYSKTLDQILASIPTILAVFETGLQ------------KLHKQGARNFWIHNTGPLGCL 241

Query: 244 AQNVARFGTAPSKLDELGCVSSHNQAAKLFNSQLHALFKKLQEQYTDGNVTYVDIYTIKS 303
           AQNVARFGT PS LDELGCVSSHNQAAKLFN QLHAL KKLQ+QYTD NVTYVDIYTIKS
Sbjct: 242 AQNVARFGTDPSNLDELGCVSSHNQAAKLFNLQLHALCKKLQKQYTDANVTYVDIYTIKS 301

Query: 304 NLIANYSRFGFEQPIMACCGYGGPPLNYDSRIVCGQTKTLNGTVVTAQGCDDSSEYINWD 363
           NLIANYSR+GFEQPIMACCGYGGPPLNYDSRIVCGQTK LNGTVVTA+GCDDSSEYINWD
Sbjct: 302 NLIANYSRYGFEQPIMACCGYGGPPLNYDSRIVCGQTKILNGTVVTAKGCDDSSEYINWD 361

Query: 364 GIHYTEAANQYVSSQILTGKYCDPPFSDKMPFLLKLKF 402
           GIHYTE ANQYVSSQIL+GKYCDPPFSDKMPFLLKLKF
Sbjct: 362 GIHYTEVANQYVSSQILSGKYCDPPFSDKMPFLLKLKF 377

BLAST of Lsi11G005090 vs. ExPASy TrEMBL
Match: A0A6J1DB59 (GDSL esterase/lipase At1g54790-like OS=Momordica charantia OX=3673 GN=LOC111018863 PE=3 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 3.8e-187
Identity = 323/402 (80.35%), Postives = 353/402 (87.81%), Query Frame = 0

Query: 1   MASAAAVFLFFL-LFLGVPMARSSHFNRPAVFNFGDSNSDTGCLVSSGIETISPPYGHLF 60
           MA  A+V L  + + LG+ +ARSS FNRPAVFNFGDSNSDTGCLVS+G ETI+PPYGH F
Sbjct: 15  MAVTASVLLILIFVLLGICVARSSPFNRPAVFNFGDSNSDTGCLVSAGFETINPPYGHRF 74

Query: 61  FGNPSGRYCDGRLILDFLLDAMDLPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSF 120
           FG+PSGRYCDGRLI+DFLLDAM++PYLN YLDS+G PNFRKGCNYAAAASTVLPATP SF
Sbjct: 75  FGHPSGRYCDGRLIVDFLLDAMNMPYLNAYLDSVGLPNFRKGCNYAAAASTVLPATPFSF 134

Query: 121 SPFSFGVQVNQFIHFKARVLELRSKGTITSTSLLLGKKLDKYLPEEDYFQKGLYMFDIGQ 180
           SPFSFGVQVNQF+HFKARVLELR+           GKK DKY+P EDYF+KGLYMFD+GQ
Sbjct: 135 SPFSFGVQVNQFLHFKARVLELRASKD--------GKKFDKYIPAEDYFEKGLYMFDMGQ 194

Query: 181 NDLAAAFYSKTLDQILASIPTILAEFETGIQSRAFLQLGMGCLRLYDQGARNFWIHNTGP 240
           NDLA+AFYSKTLDQILAS+PTILAEFE+GIQ            RLYDQGARNFWIHNTGP
Sbjct: 195 NDLASAFYSKTLDQILASVPTILAEFESGIQ------------RLYDQGARNFWIHNTGP 254

Query: 241 LGCLAQNVARFGTAPSKLDELGCVSSHNQAAKLFNSQLHALFKKLQEQYTDGNVTYVDIY 300
           LGCLAQNVA+FGT PSKLDE GCVS+HNQAAKLFNSQLHAL KKLQ Q++DGNVTYVDIY
Sbjct: 255 LGCLAQNVAKFGTDPSKLDEFGCVSAHNQAAKLFNSQLHALSKKLQAQFSDGNVTYVDIY 314

Query: 301 TIKSNLIANYSRFGFEQPIMACCGYGGPPLNYDSRIVCGQTKTLNGTVVTAQGCDDSSEY 360
           TIKSNLIANYSR GFEQPIMACCGYGGPPLNYDSR+ CGQTKTLNG  VTA+GCD+SS Y
Sbjct: 315 TIKSNLIANYSRLGFEQPIMACCGYGGPPLNYDSRVGCGQTKTLNGMGVTAKGCDNSSVY 374

Query: 361 INWDGIHYTEAANQYVSSQILTGKYCDPPFSDKMPFLLKLKF 402
           INWDGIHYTEAANQYVSS+IL+GKY DPPFSDKMP+LLKLKF
Sbjct: 375 INWDGIHYTEAANQYVSSEILSGKYSDPPFSDKMPYLLKLKF 396

BLAST of Lsi11G005090 vs. NCBI nr
Match: XP_004139196.2 (GDSL esterase/lipase At1g54790 [Cucumis sativus])

HSP 1 Score: 718.8 bits (1854), Expect = 2.7e-203
Identity = 352/401 (87.78%), Postives = 363/401 (90.52%), Query Frame = 0

Query: 1   MASAAAVFLFFLLFLGVPMARSSHFNRPAVFNFGDSNSDTGCLVSSGIETISPPYGHLFF 60
           MAS AA FLFFLLF G P ARSSHFNRPAVFNFGDSNSDTGCLVSSGIE I PPYGHLFF
Sbjct: 1   MASPAASFLFFLLFFGAPTARSSHFNRPAVFNFGDSNSDTGCLVSSGIEAIGPPYGHLFF 60

Query: 61  GNPSGRYCDGRLILDFLLDAMDLPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFS 120
           GNPSGRYCDGRLILDFLLDAMD+PYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFS
Sbjct: 61  GNPSGRYCDGRLILDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFS 120

Query: 121 PFSFGVQVNQFIHFKARVLELRSKGTITSTSLLLGKKLDKYLPEEDYFQKGLYMFDIGQN 180
           PFSFGVQVNQFIHFKARVLELRSK          GKKLDKYLP+EDYF+KGLYMFDIGQN
Sbjct: 121 PFSFGVQVNQFIHFKARVLELRSK----------GKKLDKYLPDEDYFEKGLYMFDIGQN 180

Query: 181 DLAAAFYSKTLDQILASIPTILAEFETGIQSRAFLQLGMGCLRLYDQGARNFWIHNTGPL 240
           DLA AFYSKTLDQILASIPTILA FETG+Q            +LYDQGARNFWIHNTGPL
Sbjct: 181 DLAIAFYSKTLDQILASIPTILAVFETGLQ------------KLYDQGARNFWIHNTGPL 240

Query: 241 GCLAQNVARFGTAPSKLDELGCVSSHNQAAKLFNSQLHALFKKLQEQYTDGNVTYVDIYT 300
           GCLAQNVARFGT PS LDELGCVSSHNQAAKLFN QLHAL K+LQE+YTD NVTYVDIYT
Sbjct: 241 GCLAQNVARFGTDPSNLDELGCVSSHNQAAKLFNLQLHALCKELQEEYTDTNVTYVDIYT 300

Query: 301 IKSNLIANYSRFGFEQPIMACCGYGGPPLNYDSRIVCGQTKTLNGTVVTAQGCDDSSEYI 360
           IKSNLIANYSRFGFEQPIMACCGYGGPPLNYDSRI+CGQTK LNGTVVTA+GCDDSSEYI
Sbjct: 301 IKSNLIANYSRFGFEQPIMACCGYGGPPLNYDSRIICGQTKILNGTVVTAKGCDDSSEYI 360

Query: 361 NWDGIHYTEAANQYVSSQILTGKYCDPPFSDKMPFLLKLKF 402
           NWDGIHY+EAAN+YVSSQILTGKY DPPFSDKMPFLLKLKF
Sbjct: 361 NWDGIHYSEAANKYVSSQILTGKYSDPPFSDKMPFLLKLKF 379

BLAST of Lsi11G005090 vs. NCBI nr
Match: KAE8651584.1 (hypothetical protein Csa_023443 [Cucumis sativus])

HSP 1 Score: 718.4 bits (1853), Expect = 3.5e-203
Identity = 352/401 (87.78%), Postives = 364/401 (90.77%), Query Frame = 0

Query: 1   MASAAAVFLFFLLFLGVPMARSSHFNRPAVFNFGDSNSDTGCLVSSGIETISPPYGHLFF 60
           MAS AA FLFFLLF G P ARSSHFNRPAVFNFGDSNSDTGCLVSSGIE I PPYGHLFF
Sbjct: 1   MASPAASFLFFLLFFGAPTARSSHFNRPAVFNFGDSNSDTGCLVSSGIEAIGPPYGHLFF 60

Query: 61  GNPSGRYCDGRLILDFLLDAMDLPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFS 120
           GNPSGRYCDGRLILDFLLDAMD+PYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFS
Sbjct: 61  GNPSGRYCDGRLILDFLLDAMDMPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFS 120

Query: 121 PFSFGVQVNQFIHFKARVLELRSKGTITSTSLLLGKKLDKYLPEEDYFQKGLYMFDIGQN 180
           PFSFGVQVNQFIHFKARVLELRSK          GKKLDKYLP+EDYF+KGLYMFDIGQN
Sbjct: 121 PFSFGVQVNQFIHFKARVLELRSK----------GKKLDKYLPDEDYFEKGLYMFDIGQN 180

Query: 181 DLAAAFYSKTLDQILASIPTILAEFETGIQSRAFLQLGMGCLRLYDQGARNFWIHNTGPL 240
           DLA AFYSKTLDQILASIPTILA FETG+Q     +      +LYDQGARNFWIHNTGPL
Sbjct: 181 DLAIAFYSKTLDQILASIPTILAVFETGLQPLHLPKF----QKLYDQGARNFWIHNTGPL 240

Query: 241 GCLAQNVARFGTAPSKLDELGCVSSHNQAAKLFNSQLHALFKKLQEQYTDGNVTYVDIYT 300
           GCLAQNVARFGT PS LDELGCVSSHNQAAKLFN QLHAL K+LQE+YTD NVTYVDIYT
Sbjct: 241 GCLAQNVARFGTDPSNLDELGCVSSHNQAAKLFNLQLHALCKELQEEYTDTNVTYVDIYT 300

Query: 301 IKSNLIANYSRFGFEQPIMACCGYGGPPLNYDSRIVCGQTKTLNGTVVTAQGCDDSSEYI 360
           IKSNLIANYSRFGFEQPIMACCGYGGPPLNYDSRI+CGQTK LNGTVVTA+GCDDSSEYI
Sbjct: 301 IKSNLIANYSRFGFEQPIMACCGYGGPPLNYDSRIICGQTKILNGTVVTAKGCDDSSEYI 360

Query: 361 NWDGIHYTEAANQYVSSQILTGKYCDPPFSDKMPFLLKLKF 402
           NWDGIHY+EAAN+YVSSQILTGKY DPPFSDKMPFLLKLKF
Sbjct: 361 NWDGIHYSEAANKYVSSQILTGKYSDPPFSDKMPFLLKLKF 387

BLAST of Lsi11G005090 vs. NCBI nr
Match: XP_008455108.1 (PREDICTED: GDSL esterase/lipase At1g54790 [Cucumis melo])

HSP 1 Score: 714.9 bits (1844), Expect = 3.8e-202
Identity = 348/398 (87.44%), Postives = 362/398 (90.95%), Query Frame = 0

Query: 4   AAAVFLFFLLFLGVPMARSSHFNRPAVFNFGDSNSDTGCLVSSGIETISPPYGHLFFGNP 63
           A+AVFLFFLLF G+PMARSS FNRPAVFNFGDSNSDTGCLVSSGIETI PPYGHLFFGNP
Sbjct: 78  ASAVFLFFLLFFGLPMARSSPFNRPAVFNFGDSNSDTGCLVSSGIETIGPPYGHLFFGNP 137

Query: 64  SGRYCDGRLILDFLLDAMDLPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFSPFS 123
           SGRYCDGRLILDFLLDAMD+PYLNPYLDS+GAPNFRKGCNYAAAAST+LPATPTSFSPFS
Sbjct: 138 SGRYCDGRLILDFLLDAMDMPYLNPYLDSIGAPNFRKGCNYAAAASTILPATPTSFSPFS 197

Query: 124 FGVQVNQFIHFKARVLELRSKGTITSTSLLLGKKLDKYLPEEDYFQKGLYMFDIGQNDLA 183
           FGVQVNQFIHFKARVLELRSK           KKLDKYLP+EDYF+KGLYMFDIGQNDLA
Sbjct: 198 FGVQVNQFIHFKARVLELRSK----------DKKLDKYLPDEDYFEKGLYMFDIGQNDLA 257

Query: 184 AAFYSKTLDQILASIPTILAEFETGIQSRAFLQLGMGCLRLYDQGARNFWIHNTGPLGCL 243
            AFYSKTLDQILASIPTILA FETG+Q            +L+ QGARNFWIHNTGPLGCL
Sbjct: 258 IAFYSKTLDQILASIPTILAVFETGLQ------------KLHKQGARNFWIHNTGPLGCL 317

Query: 244 AQNVARFGTAPSKLDELGCVSSHNQAAKLFNSQLHALFKKLQEQYTDGNVTYVDIYTIKS 303
           AQNVARFGT PS LDELGCVSSHNQAAKLFN QLHAL KKLQ+QYTD NVTYVDIYTIKS
Sbjct: 318 AQNVARFGTDPSNLDELGCVSSHNQAAKLFNLQLHALCKKLQKQYTDANVTYVDIYTIKS 377

Query: 304 NLIANYSRFGFEQPIMACCGYGGPPLNYDSRIVCGQTKTLNGTVVTAQGCDDSSEYINWD 363
           NLIANYSR+GFEQPIMACCGYGGPPLNYDSRIVCGQTK LNGTVVTA+GCDDSSEYINWD
Sbjct: 378 NLIANYSRYGFEQPIMACCGYGGPPLNYDSRIVCGQTKILNGTVVTAKGCDDSSEYINWD 437

Query: 364 GIHYTEAANQYVSSQILTGKYCDPPFSDKMPFLLKLKF 402
           GIHYTE ANQYVSSQIL+GKYCDPPFSDKMPFLLKLKF
Sbjct: 438 GIHYTEVANQYVSSQILSGKYCDPPFSDKMPFLLKLKF 453

BLAST of Lsi11G005090 vs. NCBI nr
Match: KAA0063678.1 (GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 711.4 bits (1835), Expect = 4.2e-201
Identity = 346/398 (86.93%), Postives = 361/398 (90.70%), Query Frame = 0

Query: 4   AAAVFLFFLLFLGVPMARSSHFNRPAVFNFGDSNSDTGCLVSSGIETISPPYGHLFFGNP 63
           A+A+FLFFLLF G+PMARSS FNRPAVFNFGDSNSDTGCLVSSGIETI PPYGHLFFGNP
Sbjct: 79  ASALFLFFLLFFGLPMARSSPFNRPAVFNFGDSNSDTGCLVSSGIETIGPPYGHLFFGNP 138

Query: 64  SGRYCDGRLILDFLLDAMDLPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFSPFS 123
           SGRYCDGRLILDFLLDAMD+PYLNPYLDS+GAPN RKGCNYAAAAST+LPATPTSFSPFS
Sbjct: 139 SGRYCDGRLILDFLLDAMDMPYLNPYLDSIGAPNLRKGCNYAAAASTILPATPTSFSPFS 198

Query: 124 FGVQVNQFIHFKARVLELRSKGTITSTSLLLGKKLDKYLPEEDYFQKGLYMFDIGQNDLA 183
           FGVQVNQFIHFKARVLELRSK           KKLDKYLP+EDYF+KGLYMFDIGQNDLA
Sbjct: 199 FGVQVNQFIHFKARVLELRSK----------DKKLDKYLPDEDYFEKGLYMFDIGQNDLA 258

Query: 184 AAFYSKTLDQILASIPTILAEFETGIQSRAFLQLGMGCLRLYDQGARNFWIHNTGPLGCL 243
            AFYSKTLDQILASIPTILA FETG+Q            +L+ QGARNFWIHNTGPLGCL
Sbjct: 259 IAFYSKTLDQILASIPTILAVFETGLQ------------KLHKQGARNFWIHNTGPLGCL 318

Query: 244 AQNVARFGTAPSKLDELGCVSSHNQAAKLFNSQLHALFKKLQEQYTDGNVTYVDIYTIKS 303
           AQNVARFGT PS LDELGCVSSHNQAAKLFN QLHAL KKLQ+QYTD NVTYVDIYTIKS
Sbjct: 319 AQNVARFGTDPSNLDELGCVSSHNQAAKLFNLQLHALCKKLQKQYTDANVTYVDIYTIKS 378

Query: 304 NLIANYSRFGFEQPIMACCGYGGPPLNYDSRIVCGQTKTLNGTVVTAQGCDDSSEYINWD 363
           NLIANYSR+GFEQPIMACCGYGGPPLNYDSRIVCGQTK LNGTVVTA+GCDDSSEYINWD
Sbjct: 379 NLIANYSRYGFEQPIMACCGYGGPPLNYDSRIVCGQTKILNGTVVTAKGCDDSSEYINWD 438

Query: 364 GIHYTEAANQYVSSQILTGKYCDPPFSDKMPFLLKLKF 402
           GIHYTE ANQYVSSQIL+GKYCDPPFSDKMPFLLKLKF
Sbjct: 439 GIHYTEVANQYVSSQILSGKYCDPPFSDKMPFLLKLKF 454

BLAST of Lsi11G005090 vs. NCBI nr
Match: TYK18349.1 (GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 711.4 bits (1835), Expect = 4.2e-201
Identity = 346/398 (86.93%), Postives = 361/398 (90.70%), Query Frame = 0

Query: 4   AAAVFLFFLLFLGVPMARSSHFNRPAVFNFGDSNSDTGCLVSSGIETISPPYGHLFFGNP 63
           A+A+FLFFLLF G+PMARSS FNRPAVFNFGDSNSDTGCLVSSGIETI PPYGHLFFGNP
Sbjct: 2   ASALFLFFLLFFGLPMARSSPFNRPAVFNFGDSNSDTGCLVSSGIETIGPPYGHLFFGNP 61

Query: 64  SGRYCDGRLILDFLLDAMDLPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFSPFS 123
           SGRYCDGRLILDFLLDAMD+PYLNPYLDS+GAPN RKGCNYAAAAST+LPATPTSFSPFS
Sbjct: 62  SGRYCDGRLILDFLLDAMDMPYLNPYLDSIGAPNLRKGCNYAAAASTILPATPTSFSPFS 121

Query: 124 FGVQVNQFIHFKARVLELRSKGTITSTSLLLGKKLDKYLPEEDYFQKGLYMFDIGQNDLA 183
           FGVQVNQFIHFKARVLELRSK           KKLDKYLP+EDYF+KGLYMFDIGQNDLA
Sbjct: 122 FGVQVNQFIHFKARVLELRSK----------DKKLDKYLPDEDYFEKGLYMFDIGQNDLA 181

Query: 184 AAFYSKTLDQILASIPTILAEFETGIQSRAFLQLGMGCLRLYDQGARNFWIHNTGPLGCL 243
            AFYSKTLDQILASIPTILA FETG+Q            +L+ QGARNFWIHNTGPLGCL
Sbjct: 182 IAFYSKTLDQILASIPTILAVFETGLQ------------KLHKQGARNFWIHNTGPLGCL 241

Query: 244 AQNVARFGTAPSKLDELGCVSSHNQAAKLFNSQLHALFKKLQEQYTDGNVTYVDIYTIKS 303
           AQNVARFGT PS LDELGCVSSHNQAAKLFN QLHAL KKLQ+QYTD NVTYVDIYTIKS
Sbjct: 242 AQNVARFGTDPSNLDELGCVSSHNQAAKLFNLQLHALCKKLQKQYTDANVTYVDIYTIKS 301

Query: 304 NLIANYSRFGFEQPIMACCGYGGPPLNYDSRIVCGQTKTLNGTVVTAQGCDDSSEYINWD 363
           NLIANYSR+GFEQPIMACCGYGGPPLNYDSRIVCGQTK LNGTVVTA+GCDDSSEYINWD
Sbjct: 302 NLIANYSRYGFEQPIMACCGYGGPPLNYDSRIVCGQTKILNGTVVTAKGCDDSSEYINWD 361

Query: 364 GIHYTEAANQYVSSQILTGKYCDPPFSDKMPFLLKLKF 402
           GIHYTE ANQYVSSQIL+GKYCDPPFSDKMPFLLKLKF
Sbjct: 362 GIHYTEVANQYVSSQILSGKYCDPPFSDKMPFLLKLKF 377

BLAST of Lsi11G005090 vs. TAIR 10
Match: AT1G54790.3 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 569.7 bits (1467), Expect = 1.9e-162
Identity = 271/396 (68.43%), Postives = 316/396 (79.80%), Query Frame = 0

Query: 6   AVFLFFLLFLGVPMARSSHFNRPAVFNFGDSNSDTGCLVSSGIETISPPYGHLFFGNPSG 65
           ++ LF L+ L +P + S     PA+ NFGDSNSDTG L+S+GIE ++PPYG  +F  PSG
Sbjct: 9   SIVLFILISLFLPSSFSIILKYPAIINFGDSNSDTGNLISAGIENVNPPYGQTYFNLPSG 68

Query: 66  RYCDGRLILDFLLDAMDLPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFSPFSFG 125
           RYCDGRLI+DFLLD MDLP+LNPYLDSLG PNF+KGCN+AAA ST+LPA PTS SPFSF 
Sbjct: 69  RYCDGRLIVDFLLDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFD 128

Query: 126 VQVNQFIHFKARVLELRSKGTITSTSLLLGKKLDKYLPEEDYFQKGLYMFDIGQNDLAAA 185
           +Q++QFI FK+R +EL SK          G+K +KYLP  DY+ KGLYM DIGQND+A A
Sbjct: 129 LQISQFIRFKSRAIELLSK---------TGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGA 188

Query: 186 FYSKTLDQILASIPTILAEFETGIQSRAFLQLGMGCLRLYDQGARNFWIHNTGPLGCLAQ 245
           FYSKTLDQ+LASIP+IL  FE G++            RLY++G RN WIHNTGPLGCLAQ
Sbjct: 189 FYSKTLDQVLASIPSILETFEAGLK------------RLYEEGGRNIWIHNTGPLGCLAQ 248

Query: 246 NVARFGTAPSKLDELGCVSSHNQAAKLFNSQLHALFKKLQEQYTDGNVTYVDIYTIKSNL 305
           N+A+FGT  +KLDE GCVSSHNQAAKLFN QLHA+  K Q QY D NVTYVDI++IKSNL
Sbjct: 249 NIAKFGTDSTKLDEFGCVSSHNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNL 308

Query: 306 IANYSRFGFEQPIMACCGYGGPPLNYDSRIVCGQTKTLNGTVVTAQGCDDSSEYINWDGI 365
           IANYSRFGFE+P+MACCG GG PLNYDSRI CGQTK L+G  VTA+ C+DSSEYINWDGI
Sbjct: 309 IANYSRFGFEKPLMACCGVGGAPLNYDSRITCGQTKVLDGISVTAKACNDSSEYINWDGI 368

Query: 366 HYTEAANQYVSSQILTGKYCDPPFSDKMPFLLKLKF 402
           HYTEAAN++VSSQILTGKY DPPFSD+MPF L LKF
Sbjct: 369 HYTEAANEFVSSQILTGKYSDPPFSDQMPFFLTLKF 383

BLAST of Lsi11G005090 vs. TAIR 10
Match: AT1G54790.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 540.0 bits (1390), Expect = 1.6e-153
Identity = 263/395 (66.58%), Postives = 306/395 (77.47%), Query Frame = 0

Query: 7   VFLFFLLFLGVPMARSSHFNRPAVFNFGDSNSDTGCLVSSGIETISPPYGHLFFGNPSGR 66
           V LFF+  L +  + S  FN P+ FNFGDSNSDTG LV+     +  P G   F   S R
Sbjct: 11  VILFFISSLQI--SNSIDFNYPSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKTSSQR 70

Query: 67  YCDGRLILDFLLDAMDLPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFSPFSFGV 126
           +CDGRL++DFL+D MDLP+LNPYLDSLG PNF+KGCN+AAA ST+LPA PTS SPFSF +
Sbjct: 71  FCDGRLVIDFLMDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDL 130

Query: 127 QVNQFIHFKARVLELRSKGTITSTSLLLGKKLDKYLPEEDYFQKGLYMFDIGQNDLAAAF 186
           Q++QFI FK+R +EL SK          G+K +KYLP  DY+ KGLYM DIGQND+A AF
Sbjct: 131 QISQFIRFKSRAIELLSK---------TGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAF 190

Query: 187 YSKTLDQILASIPTILAEFETGIQSRAFLQLGMGCLRLYDQGARNFWIHNTGPLGCLAQN 246
           YSKTLDQ+LASIP+IL  FE G++            RLY++G RN WIHNTGPLGCLAQN
Sbjct: 191 YSKTLDQVLASIPSILETFEAGLK------------RLYEEGGRNIWIHNTGPLGCLAQN 250

Query: 247 VARFGTAPSKLDELGCVSSHNQAAKLFNSQLHALFKKLQEQYTDGNVTYVDIYTIKSNLI 306
           +A+FGT  +KLDE GCVSSHNQAAKLFN QLHA+  K Q QY D NVTYVDI++IKSNLI
Sbjct: 251 IAKFGTDSTKLDEFGCVSSHNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLI 310

Query: 307 ANYSRFGFEQPIMACCGYGGPPLNYDSRIVCGQTKTLNGTVVTAQGCDDSSEYINWDGIH 366
           ANYSRFGFE+P+MACCG GG PLNYDSRI CGQTK L+G  VTA+ C+DSSEYINWDGIH
Sbjct: 311 ANYSRFGFEKPLMACCGVGGAPLNYDSRITCGQTKVLDGISVTAKACNDSSEYINWDGIH 370

Query: 367 YTEAANQYVSSQILTGKYCDPPFSDKMPFLLKLKF 402
           YTEAAN++VSSQILTGKY DPPFSD+MPF L LKF
Sbjct: 371 YTEAANEFVSSQILTGKYSDPPFSDQMPFFLTLKF 382

BLAST of Lsi11G005090 vs. TAIR 10
Match: AT1G54790.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 525.8 bits (1353), Expect = 3.1e-149
Identity = 263/421 (62.47%), Postives = 306/421 (72.68%), Query Frame = 0

Query: 7   VFLFFLLFLGVPMARSSHFNRPAVFNFGDSNSDTGCLVSSGIETISPPYGHLFFGNPSGR 66
           V LFF+  L +  + S  FN P+ FNFGDSNSDTG LV+     +  P G   F   S R
Sbjct: 11  VILFFISSLQI--SNSIDFNYPSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKTSSQR 70

Query: 67  YCDGRLILDFLLDAMDLPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFSPFSFGV 126
           +CDGRL++DFL+D MDLP+LNPYLDSLG PNF+KGCN+AAA ST+LPA PTS SPFSF +
Sbjct: 71  FCDGRLVIDFLMDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDL 130

Query: 127 QVNQFIHFKARVLELRSKGTITSTSLLLGKKLDKYLPEEDYFQKGLYMFDIGQNDLAAAF 186
           Q++QFI FK+R +EL SK          G+K +KYLP  DY+ KGLYM DIGQND+A AF
Sbjct: 131 QISQFIRFKSRAIELLSK---------TGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAF 190

Query: 187 YSKTLDQILASIPTILAEFETGIQSRAFLQLGMGCLRLYDQGARNFWIHNTGPLGCLAQN 246
           YSKTLDQ+LASIP+IL  FE G++            RLY++G RN WIHNTGPLGCLAQN
Sbjct: 191 YSKTLDQVLASIPSILETFEAGLK------------RLYEEGGRNIWIHNTGPLGCLAQN 250

Query: 247 VARFGTAPSKLDELGCVSSHNQAAKLFNSQLHALFKKLQEQYTDGNVTYVDIYTIKSNLI 306
           +A+FGT  +KLDE GCVSSHNQAAKLFN QLHA+  K Q QY D NVTYVDI++IKSNLI
Sbjct: 251 IAKFGTDSTKLDEFGCVSSHNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLI 310

Query: 307 ANYSRF--------------------------GFEQPIMACCGYGGPPLNYDSRIVCGQT 366
           ANYSRF                          GFE+P+MACCG GG PLNYDSRI CGQT
Sbjct: 311 ANYSRFGKHFTKPLIDLNHLENVGYNKILNVLGFEKPLMACCGVGGAPLNYDSRITCGQT 370

Query: 367 KTLNGTVVTAQGCDDSSEYINWDGIHYTEAANQYVSSQILTGKYCDPPFSDKMPFLLKLK 402
           K L+G  VTA+ C+DSSEYINWDGIHYTEAAN++VSSQILTGKY DPPFSD+MPF L LK
Sbjct: 371 KVLDGISVTAKACNDSSEYINWDGIHYTEAANEFVSSQILTGKYSDPPFSDQMPFFLTLK 408

BLAST of Lsi11G005090 vs. TAIR 10
Match: AT3G05180.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 439.9 bits (1130), Expect = 2.2e-123
Identity = 222/387 (57.36%), Postives = 281/387 (72.61%), Query Frame = 0

Query: 9   LFFLLFLGV-----PMARSSHFNR--PAVFNFGDSNSDTGCLVSSGIETI-SPPYGHLFF 68
           L  LLF+ +     P+A S   +   PAVFNFGDSNSDTG L SSG+  +  P Y   FF
Sbjct: 9   LRLLLFVAISHTLSPLAGSFRISNDFPAVFNFGDSNSDTGEL-SSGLGFLPQPSYEITFF 68

Query: 69  GNP-SGRYCDGRLILDFLLDAMDLPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSF 128
            +P SGR+C+GRLI+DFL++A+D PYL PYLDS+    +R+GCN+AAAAST+  A   S+
Sbjct: 69  RSPTSGRFCNGRLIVDFLMEAIDRPYLRPYLDSISRQTYRRGCNFAAAASTIQKANAASY 128

Query: 129 SPFSFGVQVNQFIHFKARVLELRSKGTITSTSLLLGKKLDKYLPEEDYFQKGLYMFDIGQ 188
           SPF FGVQV+QFI FK++VL+L          +   ++L +YLP E +F  GLYMFDIGQ
Sbjct: 129 SPFGFGVQVSQFITFKSKVLQL----------IQQDEELQRYLPSEYFFSNGLYMFDIGQ 188

Query: 189 NDLAAAFYSKTLDQILASIPTILAEFETGIQSRAFLQLGMGCLRLYDQGARNFWIHNTGP 248
           ND+A AFY+KT+DQ+LA +P IL  F+ GI+            RLY +GARN+WIHNTGP
Sbjct: 189 NDIAGAFYTKTVDQVLALVPIILDIFQDGIK------------RLYAEGARNYWIHNTGP 248

Query: 249 LGCLAQNVARFGTAPSKLDELGCVSSHNQAAKLFNSQLHALFKKLQEQYTDGNVTYVDIY 308
           LGCLAQ V+ FG   SKLDE GCVS HNQAAKLFN QLH LFKKL +QY +   TYVDI+
Sbjct: 249 LGCLAQVVSIFGEDKSKLDEFGCVSDHNQAAKLFNLQLHGLFKKLPQQYPNSRFTYVDIF 308

Query: 309 TIKSNLIANYSRFGFEQPIMACCGYGGPPLNYDSRIVCGQTKTLNGTVVTAQGCDDSSEY 368
           +IKS+LI N+S++GF+  IM CCG GGPPLNYD ++ CG+T   NGT++TA+ C DSS+Y
Sbjct: 309 SIKSDLILNHSKYGFDHSIMVCCGTGGPPLNYDDQVGCGKTARSNGTIITAKPCYDSSKY 368

Query: 369 INWDGIHYTEAANQYVSSQILTGKYCD 387
           +NWDGIHYTEAAN++V+  ILTGKY +
Sbjct: 369 VNWDGIHYTEAANRFVALHILTGKYSE 372

BLAST of Lsi11G005090 vs. TAIR 10
Match: AT1G56670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 304.7 bits (779), Expect = 1.1e-82
Identity = 167/378 (44.18%), Postives = 223/378 (58.99%), Query Frame = 0

Query: 12  LLFLGVPMARSSHFNRPAVFNFGDSNSDTGCLVSSGIETISPPYGHLFFGNPSGRYCDGR 71
           LL L  P   +S   RP +FNFGDSNSDTG LV+     I  P G LFF   +GR  DGR
Sbjct: 23  LLILRQPSRAASCTARPVIFNFGDSNSDTGGLVAGLGYPIGFPNGRLFFRRSTGRLSDGR 82

Query: 72  LILDFLLDAMDLPYLNPYLDSLGAPNFRKGCNYAAAASTVLPATPTSFSPFSFGVQVNQF 131
           L++DFL  +++   L PYLDSLG   F+ G N+A A S  LP       PFS  +QV QF
Sbjct: 83  LLIDFLCQSLNTSLLRPYLDSLGRTRFQNGANFAIAGSPTLPKN----VPFSLNIQVKQF 142

Query: 132 IHFKARVLELRSKGTITSTSLLLGKKLDKYLPEEDYFQKGLYMFDIGQNDLAAAF-YSKT 191
            HFK+R LEL S     S++ L G  +       + F+  LYM DIGQND+A +F    +
Sbjct: 143 SHFKSRSLELAS-----SSNSLKGMFI-----SNNGFKNALYMIDIGQNDIARSFARGNS 202

Query: 192 LDQILASIPTILAEFETGIQSRAFLQLGMGCLRLYDQGARNFWIHNTGPLGCLAQNVARF 251
             Q +  IP I+ E ++ I+            RLYD+G R FWIHNTGPLGCL Q ++  
Sbjct: 203 YSQTVKLIPQIITEIKSSIK------------RLYDEGGRRFWIHNTGPLGCLPQKLSM- 262

Query: 252 GTAPSKLDELGCVSSHNQAAKLFNSQLHALFKKLQEQYTDGNVTYVDIYTIKSNLIANYS 311
                 LD+ GC+ S+N AA LFN  L  + ++L+ +  D  + Y+DIY IK +LIAN +
Sbjct: 263 -VKSKDLDQHGCLVSYNSAATLFNQGLDHMCEELRTELRDATIIYIDIYAIKYSLIANSN 322

Query: 312 RFGFEQPIMACCGYGGPPLNYDSRIVCGQTKTLNGTVVTAQGCDDSSEYINWDGIHYTEA 371
           ++GF+ P+MACCGYGG P NY+ +I CG      G+ V    C++ S +I+WDGIHYTE 
Sbjct: 323 QYGFKSPLMACCGYGGTPYNYNVKITCGH----KGSNV----CEEGSRFISWDGIHYTET 364

Query: 372 ANQYVSSQILTGKYCDPP 389
           AN  V+ ++L+  Y  PP
Sbjct: 383 ANAIVAMKVLSMHYSKPP 364

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3ECP64.3e-14862.47GDSL esterase/lipase At1g54790 OS=Arabidopsis thaliana OX=3702 GN=At1g54790 PE=2... [more]
Q9MAA13.1e-12257.36GDSL esterase/lipase At3g05180 OS=Arabidopsis thaliana OX=3702 GN=At3g05180 PE=2... [more]
Q9FXB61.6e-8144.18GDSL esterase/lipase LIP-4 OS=Arabidopsis thaliana OX=3702 GN=LIP4 PE=2 SV=1[more]
O805226.0e-8143.25GDSL esterase/lipase At1g09390 OS=Arabidopsis thaliana OX=3702 GN=At1g09390 PE=2... [more]
Q9FXE52.1e-7840.36Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LIJ51.3e-20387.78Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G020920 PE=3 SV=1[more]
A0A1S3C1D31.9e-20287.44GDSL esterase/lipase At1g54790 OS=Cucumis melo OX=3656 GN=LOC103495359 PE=3 SV=1[more]
A0A5A7V7X22.1e-20186.93GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold329... [more]
A0A5D3D4712.1e-20186.93GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456... [more]
A0A6J1DB593.8e-18780.35GDSL esterase/lipase At1g54790-like OS=Momordica charantia OX=3673 GN=LOC1110188... [more]
Match NameE-valueIdentityDescription
XP_004139196.22.7e-20387.78GDSL esterase/lipase At1g54790 [Cucumis sativus][more]
KAE8651584.13.5e-20387.78hypothetical protein Csa_023443 [Cucumis sativus][more]
XP_008455108.13.8e-20287.44PREDICTED: GDSL esterase/lipase At1g54790 [Cucumis melo][more]
KAA0063678.14.2e-20186.93GDSL esterase/lipase [Cucumis melo var. makuwa][more]
TYK18349.14.2e-20186.93GDSL esterase/lipase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT1G54790.31.9e-16268.43GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G54790.11.6e-15366.58GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G54790.23.1e-14962.47GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT3G05180.12.2e-12357.36GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G56670.11.1e-8244.18GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 20..382
e-value: 3.1E-63
score: 216.1
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 30..379
e-value: 7.4E-41
score: 141.0
NoneNo IPR availablePANTHERPTHR22835ZINC FINGER FYVE DOMAIN CONTAINING PROTEINcoord: 8..401
NoneNo IPR availablePANTHERPTHR22835:SF536OS05G0401000 PROTEINcoord: 8..401
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 28..382
e-value: 1.00458E-110
score: 325.339

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi11G005090.1Lsi11G005090.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016788 hydrolase activity, acting on ester bonds