Lsi11G004520 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi11G004520
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	EF-hand domain-containing protein
Location	chr11: 4730128 .. 4735513 (-)
RNA-Seq Expression	Lsi11G004520
Synteny	Lsi11G004520

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGGTTAAAGACACTAGAGACTCCTCTTCTTTATATGAATTGAAAATTAACACATTACACATCCATCTCTAATATTTTATTCTTAAAATATAGATAATAATCATAAAAACTAGTATTTTATTCAATTCACCAACTCCTTCAGCCACCAAATCATTCTTGACGTGGAAAAACTAAAAGAATAGAACATAATTAAAAATTAAAAATAAATAAAACCTCGAGTAATAGAGTATAATATTGAATTGAAATAAATCAAACCAATATATTTTAAAGTTATGAAACGCTTTTCGTCCTAAATTTTTAGGAAAGTAGTAATTTAAGACTTGTTTAAATTGACTCTTTATAAATGTCTATAAATACTTTTTTGTATTCATAAACACTTATTTAAACATTTAAAAAGTGAATCCAAACGGATATTTAGTCTCTAAATTTTAGTTGGTAATAATTTAATCTATGTACTCCCAAATTTTTAACAATTTAATTCATAAACTTTAACAAGTAACCATTTAGTATCTATACTTTAATTTATAATGACTTAGTCTCTATACATTATAAAAGTTTTATATATGTAGATCAATAAATTGATCAGAAATCAAATATTTATTTAAAGTTATATTTAATTTTGATGAGATTTTTCGTGATAATAATTAAATTGTTAGAAATTTTAAAGTATAGAAACTAAATTACTACTTATTAAAGTTTATAGTCTAAATTGTTATAAATTTGAAAGTACATAGACTAAACGCTACCAACTATAATTCAAAAACCAAGTACGTGTTTGAGAGTAATTTTGAAATTATTGAAATCATTTTTTTCATATTTAAAATCATTTCTAAACATGCTTTTAATTATTTGAAAATTAATTTAATATTTAATTTTACATCTCAAAATTTAATTTTCATTTTATTAAAATTGGGTTTGAATTGTTAAAGTATATTTTATAGTATTTTTAAAAATGATAAAAAGTAATTTTAAAGACTCACTTCTAGTTCTAAATTATTATTTCGGATGGTTTTGAGATTAAAAATAAATTTTATTTTAACCCATATAATTTCATGGAGAAAACTGATATTTCTCAAGACAAAGGTACTCTTTGCAGCTCAAAGAGTCAATTCCGCATTTCAAGTAAACTTTTTCATCTTCTTTCTCTCTCTCCTCTCTCGAGCTTTTTTCTTCTTCCCAGAGAAAATGTCAGAAGGCGCAATAACGGTCCTAGACGGCACAAAGCTAAGAGACATTGACCTCTCTCCGCCCTTCTCCGATGGTGGTGTTACCGGAGCTCAGGTCCTTGACCTCGCCGATTCCACAGCCTCCTCGTCTCTGTTCGGGATCGCCTTGCCGGAGACTCTCAGATCATCTGCTCTTAATTGCATCGGTTTCCACGACATTGTTGCTTTCCGACGATCGGAGCTCACTCCAGAACGAGCCTCTGAGATTCTGAAGAACTATATATCTGCGATCGCCGATGGTTTACGAGGTATCTTGTTTTCTCAGTCTTACGGCGGCTGTGTTGTGTATGTATGGTGTCTAAATGCGTAATGTTCGTAAATTTCCTTATCTGAGCTCTTGGCAGCTCATCAGGAAGTATGATTAAGTGTTATTTCTTTCTTCGTGAATTTGACATTTTAGATCTTGCGAATAGTGAATTAGATAGCCTTTAAAGGACGAACAGAAAAAAAATGAAGGAGAAATTGTTTCAGCATGTTGAAATTGAACTGGTGGCTCTAATGTTGGTCTTTTGAGTGGATTCCAAACAGGTGTAGGGCGATGATTTCCAATGTGAACTAGATAATCCACTCAGAAGATATAATCTGATACTTTAGATATATAAACCTAGGTACAGATATCATATATCTGTCTGGTTCGACCATTTTAGTCTTGTAGAGAGATTGAGTATTACGTCGATAACTTTGTATTATGCTTGTGTGGTGAGAATGAAGATACTTCATGAAGTGGTATTGTGGACAAGTGCTATTATTTGAGTCTGTACAGCGCTAGCTTCCTGCTACGGACTTGCAATATGTTTGACAGAGCAGGGGTTGGAAGGCTTGGAGTTAAGTGTGAAATTTTATGGGAACCAGGGGTTTGAATAGAGTAGGAAATGATGGAATCCTAGTGTCCGGATTTGTAGCTGAAAAGTGATAAATTCCTAAGGATTTCCTTTGAGACATTTTAAAAAAAATATGGCCATTTCAGCCAACTTTAAGACATGAAGACATAAAACCATCTGATAATTGATATGACTACATCTAGACACACGGGCAGTGTTATATTCAAATAAAATATATGGAAGTGTGCAACTCTGGTTGGTATTATAATGGAAACATATTAAAAATATAAGTAGAATGCTAAACATTTTGTTATTGTTTGTAGCATTTGTGTAACTTTTTTGTAGGTTCAAATGTCGATGGACAGTGAGACTAAATTGTGACCAATGATCCATAACCTTTTTGTGGGTTATAATTCAGACTTATATTTTAGGAATGTGTCTTCTGCTATGACCATATTTCCTTTTTAAACAATGTTCTGTCACCTCCCTTTGTCATAGTGGTGTCTGTATGTGCACAAACAGTATGGCTTGAGTATGTAGTTGGCACTGGCTCATGAAATTGAGCTTGCTTTCGTGGATCATTTTTTTGTTCCAGATGGTCCTGCTGTATGTAATTGTTTATTAGGTAATAATCTGTTGGATATTTTTCTCTGATTTATTTTTATAGCGGAAGAACATTACAAATGTTTTTTTATGTTTCCAGATGATCCTCTTCTAGTGTCAATTTTGGACGGGAACACTCTACGTATTTTCTTAGATGATGAAGATGATTTTGCAATGCTAGCCGAGACCCTTTTCACTGATTTAGATACAGAAGATAAAGGAAAGATCAAGAAGAGTGAGATTAAAAATGCTCTTATCAACATGGGGGTTGAAACGGGTGTACCACCTCTCTCAGGTTTTTTCATATTTTACTTTCCTCTTTCTCTCATTTTTTTCCCCTTTTTTTTATTGTTTAATTCTTGGTAATGTACTTCCCATCGTTACTTGGTGTCAATGGACAGAATTGTATTGTACACACTTCTACAATGAATTTATTTGCACGTTGGTCTTCTTGCCCTTGCTAGTGAAGAATTTCAAAATCTTCTGCTCAATTATATCCACTGTTTCATGTGATCAACATATCATGCTTAAAGCTTCCTTAAATAAGCTTCTACGAGGATATTTTGAACCTCCTCTTAGTGGTACAGGAGTCACATAGAGCTACTATGCTAGTACAAGTCTCTAAGTATTTGCAGAAAACCCATATTGCTTTTCATTATTATTATTTATTACTACTACTATGAATGCTCTCTATGTAAAATGTGTGCAAGAATTTCTAAAATATTTTATTATCATTAAGGAGATGTAGATTAGACTTATCAATGGGAAATCTATGTTTAAAACATTCATTATGGATTTTCCTAAGTTGGTAATGGCTTTCAAGCAACTCTCTAATATGACTTAAGAACTTTAGTTTTGAGCATTTCTTCTATTTTTAGCTGATTAGTAAAGGCAGTTTACTTTACATTTGAAGTGATTACCTGTGTGCATTTGCAACAAACTTCTCTATGTAACATATTTGCGATGAAACAGAATATCCTTTGCTGAGCGACATTTTGCAAAAACATGAAGTGGAGAGTAGCAATGAATTGGGTCAAGCTCAATTTGCAGAAGTGCTTCAGGCTGTCCTTCAAGAGTTAGCAGATACTCTAGCAAACGAACCCTATGTTTTCATTCAAAATATCAAGATCACTAATGGAGCTCAGATTAAAAAGGTAAATCGTGTTGCCATTCCTTTTCTTACCTTGGACATACCTTTCAATGATATCTTTTCCTTGACATTGGAAGTGTAGTAAACCCGTTTTTATGACAAAACTATGGAAGTGTAGTAAACCCTTAATACCATTATTAGCATTATCTTCTTCGATAGTTTTACTGGCCTGACTTATCTGCATCCTACAGCCTACGTACTAAAAGGAGTCCACCCAATCAAATTGTGGATTCGTTTTAATGGATGTAATTGATTAACTCAATTGAGTAATTTTGATAAATTTAACATTATATACTATCAGTAGCAGACACTGAAAAAAATTGTGGGAATTGAAAATTTCAACGGCACATAATAAAACGGATAAGCCCCAATCTATAGTCTATTTTTTTTTCCTCTCAAGTTCTGCATTTTGGTGACCAACTTTTGGTTGTTAAAACACTATCCTTTGCACTTTATACCATGTAAAATTGCAGTTATTTTATACTTGAGGAAAATTTCCTTTCTTTTAGTCTTATTGGGTGCAGTGAGCTAGTTCACTGGAGTTGAAGTTACCAAATTCTGAGTCCATATCGATTTATGTCTAATTGGTCCACATTCAATTCTTCCAACATATTGTCTACATGAACTAGAGCCAATCCGAATTGATCTCATAAGAGATTTGCTATTGAACATGATATCGTCAAGTGTACCCATTCTAAATTAAGTGGCTTGCACTTCACATATTGTAAACTGAATGTACATACGCAATGAGATCTGGTAGTTCTTTTCTTTTCGGTATCTGAAGTTAATAAAGTTTTCTTTTCATCTTGCCTGGCTTGACTTTTAACTTTTATTTGAGAACCGACTAATTTTGAAGACATTGTAATTTCTGATGTGAGTTGCAGCTTTTGGCTGACGAGAAGCAATTCAACGACGTCATAGAAAAGCTCTGGCAGTGGCAGGGAACCCATAAAGAAGAGGATGGGGTAACAACTCTGCTAAAAATCAGGAATTACTTTGAAAAGGAATGGAAGGAACTGGGCTTGCCACCTACAGAAGCCAATGAAGCAGTGGCTCTTCTCTATGATGCAATATTTGCTGATATAGCGAAAGAGAAGCGTGATTCAATATCTGACAAAAATCAATTTGAAAAACTTGCAAAGGAGATTTTGGAGATATTTGTGGAGCAGCTTGAAGCCAGTCCTGTGTATTATGATTGTGACCGCAAATGAGCAAGGTGACCGTGTTAAATCGGGAGCTCGTATCGTCACTTAAAGAATTGGTCGTTTCTGCAACCATCATTCTCTTATGATCAGGTACGAGCATTTAAGGGTAAAGTAATTATAATAACAAACTAGAGTGGAGATAGCATGTTATCCTGAAATCATAGTCATTTTCATTATACCATACTGAATTTGAAGGCCCTTGATGTGGCCAATAGGTTCTATAACATGTCATATAAAATCAGAAGTATAAAAATACATCATTCAGATGAGATTTGGTTATGCTTCTTCTTTGTACATTTTTTTGTGAGGAACAATTTGTACTGGCAAAATTTCCTTTACTACCAAGTAAAAGCTTTTGTCTGGTGGGATTAAAATTT

mRNA sequence

ATGAAAGAGAAAATGTCAGAAGGCGCAATAACGGTCCTAGACGGCACAAAGCTAAGAGACATTGACCTCTCTCCGCCCTTCTCCGATGGTGGTGTTACCGGAGCTCAGGTCCTTGACCTCGCCGATTCCACAGCCTCCTCGTCTCTGTTCGGGATCGCCTTGCCGGAGACTCTCAGATCATCTGCTCTTAATTGCATCGGTTTCCACGACATTGTTGCTTTCCGACGATCGGAGCTCACTCCAGAACGAGCCTCTGAGATTCTGAAGAACTATATATCTGCGATCGCCGATGGTTTACGAGATGATCCTCTTCTAGTGTCAATTTTGGACGGGAACACTCTACAAGTGCTTCAGGCTGTCCTTCAAGAGTTAGCAGATACTCTAGCAAACGAACCCTATGTTTTCATTCAAAATATCAAGATCACTAATGGAGCTCAGATTAAAAAGCTTTTGGCTGACGAGAAGCAATTCAACGACGTCATAGAAAAGCTCTGGCAGTGGCAGGGAACCCATAAAGAAGAGGATGGGGTAACAACTCTGCTAAAAATCAGGAATTACTTTGAAAAGGAATGGAAGGAACTGGGCTTGCCACCTACAGAAGCCAATGAAGCAGTGGCTCTTCTCTATGATGCAATATTTGCTGATATAGCGAAAGAGAAGCGTGATTCAATATCTGACAAAAATCAATTTGAAAAACTTGCAAAGGAGATTTTGGAGATATTTGTGGAGCAGCTTGAAGCCAGTCCTGTGTATTATGATTGTGACCGCAAATGAGCAAGGTGACCGTGTTAAATCGGGAGCTCGTATCGTCACTTAAAGAATTGGTCGTTTCTGCAACCATCATTCTCTTATGATCAGGTACGAGCATTTAAGGGTAAAGTAATTATAATAACAAACTAGAGTGGAGATAGCATGTTATCCTGAAATCATAGTCATTTTCATTATACCATACTGAATTTGAAGGCCCTTGATGTGGCCAATAGGTTCTATAACATGTCATATAAAATCAGAAGTATAAAAATACATCATTCAGATGAGATTTGGTTATGCTTCTTCTTTGTACATTTTTTTGTGAGGAACAATTTGTACTGGCAAAATTTCCTTTACTACCAAGTAAAAGCTTTTGTCTGGTGGGATTAAAATTT

Coding sequence (CDS)

ATGAAAGAGAAAATGTCAGAAGGCGCAATAACGGTCCTAGACGGCACAAAGCTAAGAGACATTGACCTCTCTCCGCCCTTCTCCGATGGTGGTGTTACCGGAGCTCAGGTCCTTGACCTCGCCGATTCCACAGCCTCCTCGTCTCTGTTCGGGATCGCCTTGCCGGAGACTCTCAGATCATCTGCTCTTAATTGCATCGGTTTCCACGACATTGTTGCTTTCCGACGATCGGAGCTCACTCCAGAACGAGCCTCTGAGATTCTGAAGAACTATATATCTGCGATCGCCGATGGTTTACGAGATGATCCTCTTCTAGTGTCAATTTTGGACGGGAACACTCTACAAGTGCTTCAGGCTGTCCTTCAAGAGTTAGCAGATACTCTAGCAAACGAACCCTATGTTTTCATTCAAAATATCAAGATCACTAATGGAGCTCAGATTAAAAAGCTTTTGGCTGACGAGAAGCAATTCAACGACGTCATAGAAAAGCTCTGGCAGTGGCAGGGAACCCATAAAGAAGAGGATGGGGTAACAACTCTGCTAAAAATCAGGAATTACTTTGAAAAGGAATGGAAGGAACTGGGCTTGCCACCTACAGAAGCCAATGAAGCAGTGGCTCTTCTCTATGATGCAATATTTGCTGATATAGCGAAAGAGAAGCGTGATTCAATATCTGACAAAAATCAATTTGAAAAACTTGCAAAGGAGATTTTGGAGATATTTGTGGAGCAGCTTGAAGCCAGTCCTGTGTATTATGATTGTGACCGCAAATGA

Protein sequence

MKEKMSEGAITVLDGTKLRDIDLSPPFSDGGVTGAQVLDLADSTASSSLFGIALPETLRSSALNCIGFHDIVAFRRSELTPERASEILKNYISAIADGLRDDPLLVSILDGNTLQVLQAVLQELADTLANEPYVFIQNIKITNGAQIKKLLADEKQFNDVIEKLWQWQGTHKEEDGVTTLLKIRNYFEKEWKELGLPPTEANEAVALLYDAIFADIAKEKRDSISDKNQFEKLAKEILEIFVEQLEASPVYYDCDRK

Homology

[Close]

BLAST of Lsi11G004520 vs. ExPASy TrEMBL
Match: A0A0A0LID8 (EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G011470 PE=4 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 7.4e-104
Identity = 216/327 (66.06%), Postives = 230/327 (70.34%), Query Frame = 0


Query: 5   MSEGAITVLDGTKLRDIDLSPPFSDGGVTGAQVLDLADSTASSSLFGIALPETLRSSALN 64
           M+EG ITVLDGT LRDIDL+PPFSD  +TGA +LDLADSTASSSLFGI LPETL+SSALN
Sbjct: 1   MAEGPITVLDGTHLRDIDLTPPFSDSVLTGAHLLDLADSTASSSLFGIPLPETLKSSALN 60

Query: 65  CIGFHDIVAFRRSELTPERASEILKNYISAIADGLRDDPLLVSILDGNTL---------- 124
            IG HDIVAFRRSELT +RAS+ILK+Y+SAIAD LRDDPLLVSILDGNTL          
Sbjct: 61  IIGLHDIVAFRRSELTSQRASQILKDYVSAIADILRDDPLLVSILDGNTLRIFLDDEDDF 120

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 121 AMLAETLFTDLDTEDKGKIKKSEIKNALVHMGVETGVPPLSEYPLLSDILQKHEVESSTE 180

Query: 185 -------QVLQAVLQELADTLANEPYVFIQNIKITNGAQIKKLLADEKQFNDVIEKLWQW 244
                  +VLQAVLQELAD LA +PYVFIQNIKITNGAQ+KKLL DEKQFNDVIEKLWQW
Sbjct: 181 LGQAQYAEVLQAVLQELADALAKKPYVFIQNIKITNGAQVKKLLTDEKQFNDVIEKLWQW 240

Query: 245 QGTHKEEDGVTTLLKIRNYFEKEWKELGL-PPTEANEAVALLYDAIFADIAKEKRDSISD 254
           QGTHKEE+GVTTL KIRNYFEKEWKELGL PPTE NEA+ LLYD IFADIAKEK DSISD
Sbjct: 241 QGTHKEENGVTTLQKIRNYFEKEWKELGLPPPTETNEAIVLLYDEIFADIAKEKCDSISD 300

[Close]

BLAST of Lsi11G004520 vs. ExPASy TrEMBL
Match: A0A6J1KH09 (uncharacterized protein LOC111493790 OS=Cucurbita maxima OX=3661 GN=LOC111493790 PE=4 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 1.5e-101
Identity = 212/330 (64.24%), Postives = 230/330 (69.70%), Query Frame = 0


Query: 5   MSEGAITVLDGTKLRDIDLSPPFSDGGVTGAQVLDLADSTASSSLFGIALPETLRSSALN 64
           MSE  IT+LDGT+LR IDLS PFSDG V+GAQVLDLADS+ SSSLF IAL ETLRSSALN
Sbjct: 1   MSETTITILDGTQLRSIDLSLPFSDGSVSGAQVLDLADSSVSSSLFEIALSETLRSSALN 60

Query: 65  CIGFHDIVAFRRSELTPERASEILKNYISAIADGLRDDPLLVSILDGNTL---------- 124
            IGFHDIVAFRRSELT ERASEILK Y++AIADGLRDDP++VSILDGN L          
Sbjct: 61  RIGFHDIVAFRRSELTRERASEILKAYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDF 120

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 121 AMLAESLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNE 180

Query: 185 -------QVLQAVLQELADTLANEPYVFIQNIKITNGAQIKKLLADEKQFNDVIEKLWQW 244
                  +VLQ VLQELADTLA +PYVFIQ+IK+ NGA+IKKLLADEKQF++VIEKLWQW
Sbjct: 181 LGQAQFAEVLQPVLQELADTLAKKPYVFIQSIKVANGARIKKLLADEKQFSNVIEKLWQW 240

Query: 245 QGTHKEEDGVTTLLKIRNYFEKEWKELGLPPTEANEAVALLYDAIFADIAKEKRDSISDK 258
           QGTHKEED VTT  KIRNYFEKEW+ELGLPPTEAN+AV LLYDAIFADIAKEK  SIS+K
Sbjct: 241 QGTHKEEDEVTTSQKIRNYFEKEWRELGLPPTEANDAVVLLYDAIFADIAKEKCGSISEK 300

[Close]

BLAST of Lsi11G004520 vs. ExPASy TrEMBL
Match: A0A5A7V5V0 (Calcium-binding EF hand family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G001390 PE=4 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 3.8e-100
Identity = 213/327 (65.14%), Postives = 227/327 (69.42%), Query Frame = 0


Query: 5   MSEGAITVLDGTKLRDIDLSPPFSDGGVTGAQVLDLADSTASSSLFGIALPETLRSSALN 64
           MSEG ITVLDGT LRDIDL+PPFSD  +TGA +LDLADST SSSLF I LPE L+SSALN
Sbjct: 1   MSEGPITVLDGTHLRDIDLTPPFSDALLTGAHLLDLADSTVSSSLFRIPLPEALKSSALN 60

Query: 65  CIGFHDIVAFRRSELTPERASEILKNYISAIADGLRDDPLLVSILDGNTL---------- 124
            IG HDIVAFRRSELTP+RASEILK+Y+SAIAD LRDDPLLVSILDG+TL          
Sbjct: 61  IIGLHDIVAFRRSELTPQRASEILKDYVSAIADILRDDPLLVSILDGSTLRIFLDDEDDF 120

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 121 AMLAETLFTDLDTEDKGKIKKSEIKNALVHMGVETGVPPLSEYPLLRDILQKHEVESSTE 180

Query: 185 -------QVLQAVLQELADTLANEPYVFIQNIKITNGAQIKKLLADEKQFNDVIEKLWQW 244
                  +VLQAVLQELAD LA +PYVFIQNIKITNGAQIKKLLAD+KQFNDVIEKLWQW
Sbjct: 181 LGQAQFAEVLQAVLQELADALAKKPYVFIQNIKITNGAQIKKLLADKKQFNDVIEKLWQW 240

Query: 245 QGTHKEEDGVTTLLKIRNYFEKEWKELGL-PPTEANEAVALLYDAIFADIAKEKRDSISD 254
           QGT+KEE+GVTTL KIRNYFEKEWKELGL PPTE NEAV LLYD IFADIAKEK  SI+D
Sbjct: 241 QGTYKEENGVTTLQKIRNYFEKEWKELGLPPPTETNEAVVLLYDEIFADIAKEKCGSITD 300

[Close]

BLAST of Lsi11G004520 vs. ExPASy TrEMBL
Match: A0A6J1G547 (uncharacterized protein LOC111450774 OS=Cucurbita moschata OX=3662 GN=LOC111450774 PE=4 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 3.8e-100
Identity = 211/330 (63.94%), Postives = 228/330 (69.09%), Query Frame = 0


Query: 5   MSEGAITVLDGTKLRDIDLSPPFSDGGVTGAQVLDLADSTASSSLFGIALPETLRSSALN 64
           MSE  IT+LDGT+LR IDLS  FSD  VTGAQVLDLADS+ SSSLFGIALPETLRSSALN
Sbjct: 1   MSEATITILDGTQLRSIDLSLLFSDRSVTGAQVLDLADSSVSSSLFGIALPETLRSSALN 60

Query: 65  CIGFHDIVAFRRSELTPERASEILKNYISAIADGLRDDPLLVSILDGNTL---------- 124
            IGFHDIVAFRRSELT ERASEILK Y++AIADGLRDDP++VSILDGN L          
Sbjct: 61  RIGFHDIVAFRRSELTRERASEILKAYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDF 120

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 121 AMLAESLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNE 180

Query: 185 -------QVLQAVLQELADTLANEPYVFIQNIKITNGAQIKKLLADEKQFNDVIEKLWQW 244
                  +VLQ VLQELADTLA +PYVFIQ+IK+ NGA+IKKLLADEKQF++VIEKLWQW
Sbjct: 181 LGQAQFAEVLQPVLQELADTLAKKPYVFIQSIKVANGARIKKLLADEKQFSNVIEKLWQW 240

Query: 245 QGTHKEEDGVTTLLKIRNYFEKEWKELGLPPTEANEAVALLYDAIFADIAKEKRDSISDK 258
           QGTHKEED VTT   IRNYFEKEW+ELGLPPTEAN+AV LLYDAIFADIAKEK  SIS+K
Sbjct: 241 QGTHKEEDEVTTSQNIRNYFEKEWRELGLPPTEANDAVVLLYDAIFADIAKEKCGSISEK 300

[Close]

BLAST of Lsi11G004520 vs. ExPASy TrEMBL
Match: A0A1S3C1Z5 (uncharacterized protein LOC103495980 OS=Cucumis melo OX=3656 GN=LOC103495980 PE=4 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 7.2e-99
Identity = 212/327 (64.83%), Postives = 226/327 (69.11%), Query Frame = 0


Query: 5   MSEGAITVLDGTKLRDIDLSPPFSDGGVTGAQVLDLADSTASSSLFGIALPETLRSSALN 64
           MSEG ITVLDGT LRDIDL+P FSD  +TGA +LDLADST SSSLF I LPE L+SSALN
Sbjct: 1   MSEGPITVLDGTHLRDIDLTPHFSDALLTGAHLLDLADSTVSSSLFRIPLPEALKSSALN 60

Query: 65  CIGFHDIVAFRRSELTPERASEILKNYISAIADGLRDDPLLVSILDGNTL---------- 124
            IG HDIVAFRRSELTP+RASEILK+Y+SAIAD LRDDPLLVSILDG+TL          
Sbjct: 61  IIGLHDIVAFRRSELTPQRASEILKDYVSAIADILRDDPLLVSILDGSTLRIFLDDEDDF 120

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 121 AMLAETLFTDLDTEDKGKIKKSEIKNALVHMGVETGVPPLSEYPLLRDILQKHEVESSTE 180

Query: 185 -------QVLQAVLQELADTLANEPYVFIQNIKITNGAQIKKLLADEKQFNDVIEKLWQW 244
                  +VLQAVLQELAD LA +PYVFIQNIKITNGAQIKKLLAD+KQFNDVIEKLWQW
Sbjct: 181 LGQAQFAEVLQAVLQELADALAKKPYVFIQNIKITNGAQIKKLLADKKQFNDVIEKLWQW 240

Query: 245 QGTHKEEDGVTTLLKIRNYFEKEWKELGL-PPTEANEAVALLYDAIFADIAKEKRDSISD 254
           QGT+KEE+GVTTL KIRNYFEKEWKELGL PPTE NEAV LLYD IFADIAKEK  SI+D
Sbjct: 241 QGTYKEENGVTTLQKIRNYFEKEWKELGLPPPTETNEAVVLLYDEIFADIAKEKCCSITD 300

[Close]

BLAST of Lsi11G004520 vs. NCBI nr
Match: XP_038891233.1 (uncharacterized protein LOC120080587 [Benincasa hispida])

HSP 1 Score: 421.0 bits (1081), Expect = 7.3e-114
Identity = 232/330 (70.30%), Postives = 240/330 (72.73%), Query Frame = 0


Query: 5   MSEGAITVLDGTKLRDIDLSPPFSDGGVTGAQVLDLADSTASSSLFGIALPETLRSSALN 64
           MSEGAITVLDGT+LRDIDLSPPFSDG VTGAQVLDLADST SSSLFGIALP TLRSS LN
Sbjct: 1   MSEGAITVLDGTQLRDIDLSPPFSDGAVTGAQVLDLADSTVSSSLFGIALPGTLRSSTLN 60

Query: 65  CIGFHDIVAFRRSELTPERASEILKNYISAIADGLRDDPLLVSILDGNTL---------- 124
            IG HDIVAFR+S+LTPERASEILK+Y+SAIADGLRDDPLLVSILDGNTL          
Sbjct: 61  TIGLHDIVAFRQSQLTPERASEILKDYVSAIADGLRDDPLLVSILDGNTLHIFLDDEDDF 120

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 121 AMLAETLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNE 180

Query: 185 -------QVLQAVLQELADTLANEPYVFIQNIKITNGAQIKKLLADEKQFNDVIEKLWQW 244
                  +VLQAVLQELADTLA +PYVFIQNIKITNGAQIKKLLADEKQFNDVIEKLWQW
Sbjct: 181 LGQAQFAEVLQAVLQELADTLAKKPYVFIQNIKITNGAQIKKLLADEKQFNDVIEKLWQW 240

Query: 245 QGTHKEEDGVTTLLKIRNYFEKEWKELGLPPTEANEAVALLYDAIFADIAKEKRDSISDK 258
           QGTHKEEDGVTTL KIRNYFEKEWKELGLPPTEANEAV LLYDAIFADIAKEK  SISDK
Sbjct: 241 QGTHKEEDGVTTLQKIRNYFEKEWKELGLPPTEANEAVVLLYDAIFADIAKEKCGSISDK 300

[Close]

BLAST of Lsi11G004520 vs. NCBI nr
Match: XP_004139231.1 (uncharacterized protein LOC101222315 [Cucumis sativus] >KGN60814.1 hypothetical protein Csa_019364 [Cucumis sativus])

HSP 1 Score: 386.7 bits (992), Expect = 1.5e-103
Identity = 216/327 (66.06%), Postives = 230/327 (70.34%), Query Frame = 0


Query: 5   MSEGAITVLDGTKLRDIDLSPPFSDGGVTGAQVLDLADSTASSSLFGIALPETLRSSALN 64
           M+EG ITVLDGT LRDIDL+PPFSD  +TGA +LDLADSTASSSLFGI LPETL+SSALN
Sbjct: 1   MAEGPITVLDGTHLRDIDLTPPFSDSVLTGAHLLDLADSTASSSLFGIPLPETLKSSALN 60

Query: 65  CIGFHDIVAFRRSELTPERASEILKNYISAIADGLRDDPLLVSILDGNTL---------- 124
            IG HDIVAFRRSELT +RAS+ILK+Y+SAIAD LRDDPLLVSILDGNTL          
Sbjct: 61  IIGLHDIVAFRRSELTSQRASQILKDYVSAIADILRDDPLLVSILDGNTLRIFLDDEDDF 120

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 121 AMLAETLFTDLDTEDKGKIKKSEIKNALVHMGVETGVPPLSEYPLLSDILQKHEVESSTE 180

Query: 185 -------QVLQAVLQELADTLANEPYVFIQNIKITNGAQIKKLLADEKQFNDVIEKLWQW 244
                  +VLQAVLQELAD LA +PYVFIQNIKITNGAQ+KKLL DEKQFNDVIEKLWQW
Sbjct: 181 LGQAQYAEVLQAVLQELADALAKKPYVFIQNIKITNGAQVKKLLTDEKQFNDVIEKLWQW 240

Query: 245 QGTHKEEDGVTTLLKIRNYFEKEWKELGL-PPTEANEAVALLYDAIFADIAKEKRDSISD 254
           QGTHKEE+GVTTL KIRNYFEKEWKELGL PPTE NEA+ LLYD IFADIAKEK DSISD
Sbjct: 241 QGTHKEENGVTTLQKIRNYFEKEWKELGLPPPTETNEAIVLLYDEIFADIAKEKCDSISD 300

[Close]

BLAST of Lsi11G004520 vs. NCBI nr
Match: XP_023547272.1 (uncharacterized protein LOC111806141 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 380.6 bits (976), Expect = 1.1e-101
Identity = 212/330 (64.24%), Postives = 231/330 (70.00%), Query Frame = 0


Query: 5   MSEGAITVLDGTKLRDIDLSPPFSDGGVTGAQVLDLADSTASSSLFGIALPETLRSSALN 64
           MSE  IT+LDGT+LR IDLS  FSD  VTGAQVLDLADS+ SSSLFGIAL ETLRSSALN
Sbjct: 1   MSEATITILDGTQLRSIDLSLSFSDRSVTGAQVLDLADSSVSSSLFGIALSETLRSSALN 60

Query: 65  CIGFHDIVAFRRSELTPERASEILKNYISAIADGLRDDPLLVSILDGNTL---------- 124
            IGFHDIVAFRRSELT ERASEILK+Y++AIADGLRDDP++VSILDGN L          
Sbjct: 61  RIGFHDIVAFRRSELTRERASEILKDYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDF 120

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 121 AMLAETLFTDLDTEDKGKIKKSEVKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNE 180

Query: 185 -------QVLQAVLQELADTLANEPYVFIQNIKITNGAQIKKLLADEKQFNDVIEKLWQW 244
                  +VLQ VLQELADTLA +PYVFI++IK+ NGAQIKKLLADEKQF++VIEKLWQW
Sbjct: 181 LGQAQFAEVLQPVLQELADTLAKKPYVFIRSIKVANGAQIKKLLADEKQFSNVIEKLWQW 240

Query: 245 QGTHKEEDGVTTLLKIRNYFEKEWKELGLPPTEANEAVALLYDAIFADIAKEKRDSISDK 258
           QGTHKEED VTTL KIRNYFEKEW+ELGLPPTEAN+A+ LLYDAIFADIAKEK  SIS+K
Sbjct: 241 QGTHKEEDEVTTLQKIRNYFEKEWRELGLPPTEANDAMVLLYDAIFADIAKEKCGSISEK 300

[Close]

BLAST of Lsi11G004520 vs. NCBI nr
Match: XP_022999409.1 (uncharacterized protein LOC111493790 [Cucurbita maxima])

HSP 1 Score: 379.0 bits (972), Expect = 3.2e-101
Identity = 212/330 (64.24%), Postives = 230/330 (69.70%), Query Frame = 0


Query: 5   MSEGAITVLDGTKLRDIDLSPPFSDGGVTGAQVLDLADSTASSSLFGIALPETLRSSALN 64
           MSE  IT+LDGT+LR IDLS PFSDG V+GAQVLDLADS+ SSSLF IAL ETLRSSALN
Sbjct: 1   MSETTITILDGTQLRSIDLSLPFSDGSVSGAQVLDLADSSVSSSLFEIALSETLRSSALN 60

Query: 65  CIGFHDIVAFRRSELTPERASEILKNYISAIADGLRDDPLLVSILDGNTL---------- 124
            IGFHDIVAFRRSELT ERASEILK Y++AIADGLRDDP++VSILDGN L          
Sbjct: 61  RIGFHDIVAFRRSELTRERASEILKAYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDF 120

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 121 AMLAESLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEYPLLSDILQKHEVESSNE 180

Query: 185 -------QVLQAVLQELADTLANEPYVFIQNIKITNGAQIKKLLADEKQFNDVIEKLWQW 244
                  +VLQ VLQELADTLA +PYVFIQ+IK+ NGA+IKKLLADEKQF++VIEKLWQW
Sbjct: 181 LGQAQFAEVLQPVLQELADTLAKKPYVFIQSIKVANGARIKKLLADEKQFSNVIEKLWQW 240

Query: 245 QGTHKEEDGVTTLLKIRNYFEKEWKELGLPPTEANEAVALLYDAIFADIAKEKRDSISDK 258
           QGTHKEED VTT  KIRNYFEKEW+ELGLPPTEAN+AV LLYDAIFADIAKEK  SIS+K
Sbjct: 241 QGTHKEEDEVTTSQKIRNYFEKEWRELGLPPTEANDAVVLLYDAIFADIAKEKCGSISEK 300

[Close]

BLAST of Lsi11G004520 vs. NCBI nr
Match: KAG6599303.1 (hypothetical protein SDJN03_09081, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 375.6 bits (963), Expect = 3.5e-100
Identity = 212/330 (64.24%), Postives = 229/330 (69.39%), Query Frame = 0


Query: 5   MSEGAITVLDGTKLRDIDLSPPFSDGGVTGAQVLDLADSTASSSLFGIALPETLRSSALN 64
           MSE  IT+LDGT+LR IDLS  FSD  VTGAQVLDLADS+ SSSLFGIALPETLRSSALN
Sbjct: 1   MSEATITILDGTQLRAIDLSLLFSDRSVTGAQVLDLADSSVSSSLFGIALPETLRSSALN 60

Query: 65  CIGFHDIVAFRRSELTPERASEILKNYISAIADGLRDDPLLVSILDGNTL---------- 124
            IGFHDIVAFRRSELT ERASEILK Y++AIADGLRDDP++VSILDGN L          
Sbjct: 61  RIGFHDIVAFRRSELTRERASEILKAYVAAIADGLRDDPVVVSILDGNNLRVFLDDEDDF 120

Query: 125 ------------------------------------------------------------ 184
                                                                       
Sbjct: 121 AMLAESLFTDLDTEDKGKIKKSEIKNALIHMGVETGVPPLSEYPSLSDILQKHEVESSNE 180

Query: 185 -------QVLQAVLQELADTLANEPYVFIQNIKITNGAQIKKLLADEKQFNDVIEKLWQW 244
                  +VLQ VLQELADTLA +PYVFI++IK+ NGAQIKKLLADEKQF++VIEKLWQW
Sbjct: 181 LGQAQFAEVLQPVLQELADTLAKKPYVFIRSIKVANGAQIKKLLADEKQFSNVIEKLWQW 240

Query: 245 QGTHKEEDGVTTLLKIRNYFEKEWKELGLPPTEANEAVALLYDAIFADIAKEKRDSISDK 258
           QGTHKEED VTT  KIRNYFEKEW+ELGLPPTEAN+AV LLYDAIFADIAKEK  SIS+K
Sbjct: 241 QGTHKEEDEVTTSQKIRNYFEKEWRELGLPPTEANDAVVLLYDAIFADIAKEKCGSISEK 300

[Close]

BLAST of Lsi11G004520 vs. TAIR 10
Match: AT5G28830.1 (calcium-binding EF hand family protein )

HSP 1 Score: 142.5 bits (358), Expect = 4.7e-34
Identity = 101/320 (31.56%), Postives = 151/320 (47.19%), Query Frame = 0


Query: 10  ITVLDGTKLRDIDLSPPFSDGGVTGAQVLDLADSTASSSLFGIALPETLRSSALNCIGFH 69
           +T+ DG  LR IDL+ P     VTGAQ+L++++S  S SL G++LP  L+ +A++ +   
Sbjct: 7   LTIFDGDLLRSIDLNLPEHQHRVTGAQLLEISESKVSQSLSGLSLPPHLKETAISQVSDG 66

Query: 70  DIVAFRRSELTPERASEILKNYISAIADGLRDDPLLVSILDGNTL--------------- 129
           D V FRR+    ++ASE L  + S +AD L+D P++VSILDG  L               
Sbjct: 67  DHVTFRRTMFNKQQASEKLGVFFSTVADALKDTPIVVSILDGTMLKMFLEDEDDFAMLAE 126

Query: 130 ------------------------------------------------------------ 189
                                                                       
Sbjct: 127 NLFTDLDEEDKGKLCKSEIRKALVHMGVEMGVPPLSEFPILDDIIKKHDADSDEELGQAQ 186

Query: 190 --QVLQAVLQELADTLANEPYVFIQNIKITNGAQIKKLLADEKQFNDVIEKLWQWQGTHK 249
             ++LQ VLQE+AD L  +P   + N++I  G++I+K+LADEK    ++EK    +   K
Sbjct: 187 FAELLQQVLQEIADVLHEKPITIVLNVEIFTGSRIRKILADEKTLKCLVEKTILEESNGK 246

Query: 250 EEDGVTTLLKIRNYFEKEWKELGLPP-TEANEAVALLYDAIFADIAKEKRDSISDKNQFE 252
           E  G    L I+N      KELGLPP +  NE VALLY+ I + +    +++ S K +F 
Sbjct: 247 ESQGWLRTLIIKN-----GKELGLPPLSSENEQVALLYETIISQL--NNKENASTKEEFM 306

The following BLAST results are available for this feature:

BLAST of Lsi11G004520 vs.


Match Name	E-value	Identity	Description

BLAST of Lsi11G004520 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LID8	7.4e-104	66.06	EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G011470 PE=...	[more]
A0A6J1KH09	1.5e-101	64.24	uncharacterized protein LOC111493790 OS=Cucurbita maxima OX=3661 GN=LOC111493790...	[more]
A0A5A7V5V0	3.8e-100	65.14	Calcium-binding EF hand family protein OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A6J1G547	3.8e-100	63.94	uncharacterized protein LOC111450774 OS=Cucurbita moschata OX=3662 GN=LOC1114507...	[more]
A0A1S3C1Z5	7.2e-99	64.83	uncharacterized protein LOC103495980 OS=Cucumis melo OX=3656 GN=LOC103495980 PE=...	[more]

BLAST of Lsi11G004520 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038891233.1	7.3e-114	70.30	uncharacterized protein LOC120080587 [Benincasa hispida]	[more]
XP_004139231.1	1.5e-103	66.06	uncharacterized protein LOC101222315 [Cucumis sativus] >KGN60814.1 hypothetical ...	[more]
XP_023547272.1	1.1e-101	64.24	uncharacterized protein LOC111806141 [Cucurbita pepo subsp. pepo]	[more]
XP_022999409.1	3.2e-101	64.24	uncharacterized protein LOC111493790 [Cucurbita maxima]	[more]
KAG6599303.1	3.5e-100	64.24	hypothetical protein SDJN03_09081, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Lsi11G004520 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G28830.1	4.7e-34	31.56	calcium-binding EF hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34574:SF3	CALCIUM-BINDING EF HAND FAMILY PROTEIN	coord: 5..117 coord: 115..255
None	No IPR available	PANTHER	PTHR34574	CALCIUM-BINDING EF-HAND FAMILY PROTEIN-RELATED	coord: 115..255
None	No IPR available	PANTHER	PTHR34574	CALCIUM-BINDING EF-HAND FAMILY PROTEIN-RELATED	coord: 5..117

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G004520.1	Lsi11G004520.1	mRNA