Lsi11G003740 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi11G003740
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionchitotriosidase-1-like
Locationchr11: 3843738 .. 3844829 (+)
RNA-Seq ExpressionLsi11G003740
SyntenyLsi11G003740
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCAAATATGTTAGCAGCTGCACCTCCCATCACTGATGTTGTGAAAGCTGGTTACTGGCTAGCAACCACTGACCCTATACTTCCTTCCGCCAATATCGATGCTATGCTTTTCACTCATCTCTTTGTTGGTTACGCTTCTGTTGACTCTACAACTTATAAAGTTACCATAGATTCTTCACAACTTGTTCTAATCAATCAGTTTACTTCAACTGTCACATTAAGTAATCCCGACGTCAAAACTTTGTTGTCTATTCAAGGATCCAATGACGTTTTTGCTCAAATTGCCAAGGATCCAACAAATCGTGATGTGTTCATCAAATCCTCCATTGAAGCAGCAAGAGCAGGTTCCTTTGATGGCCTTGATCTTCAATGGCTATATCCATCTTCAACTGAAGAAATGAAAAACTTTGAAACTTTTCTTGTTTTATGGCGCAAAGCTGTTGTGGAAGATGCCAAAAATTTGAGTGTTCCAGAGCTCCTCCTTGTTGCTTCAGTGTCCAACCTACCATATCTCACCCACAATATTCAATATCCAATTCAAACAATCAAACAAAAGTTAAATTGGGTGAATGTGATATCTTATGATTTTTACACTCCAACTTCCTCAAAGGATGCTACAGGACCATCCTCAGCATTCAACAACCCAAAAGCCAATGCCTTATCTGCAAATTTTGGTATAAAGTCATGGATTCAAGCCCTTGGAAATCTGCCTCGTAATCAGATTGTATTTGGAATTCCTTTCCATGGTTGGGCTTGGAAGCTCTCCAACTCTCACCAACATGATGTGTTTTCTAAAGCGGATGGTGCAGCTCAAGGAGACTATATTTTAGAAGGTGGAGAAATTTACTACTCCAATGTGAAGAAGTTTATTGATGATAATCAAGCTGAAGTTGTGAACAATGACAAGAGGTTTCTCATTGCATACACTTATTTTGACACAACATGGATTGCATTTGAAAGTGAATACACAATTGCAGATAAGATTTCATTGGCAAAGTCCATTAAAGTGCTTGGTTACTTTGCTTCCAACATTGCAGCTGATGATGATGTTAACACTCTTGCTAATGCTGCTCTTGCTAATTGGTGA

mRNA sequence

ATGGATTCAAATATGTTAGCAGCTGCACCTCCCATCACTGATGTTGTGAAAGCTGGTTACTGGCTAGCAACCACTGACCCTATACTTCCTTCCGCCAATATCGATGCTATGCTTTTCACTCATCTCTTTGTTGGTTACGCTTCTGTTGACTCTACAACTTATAAAGTTACCATAGATTCTTCACAACTTGTTCTAATCAATCAGTTTACTTCAACTGTCACATTAAGTAATCCCGACGTCAAAACTTTGTTGTCTATTCAAGGATCCAATGACGTTTTTGCTCAAATTGCCAAGGATCCAACAAATCGTGATGTGTTCATCAAATCCTCCATTGAAGCAGCAAGAGCAGGTTCCTTTGATGGCCTTGATCTTCAATGGCTATATCCATCTTCAACTGAAGAAATGAAAAACTTTGAAACTTTTCTTGTTTTATGGCGCAAAGCTGTTGTGGAAGATGCCAAAAATTTGAGTGTTCCAGAGCTCCTCCTTGTTGCTTCAGTGTCCAACCTACCATATCTCACCCACAATATTCAATATCCAATTCAAACAATCAAACAAAAGTTAAATTGGGTGAATGTGATATCTTATGATTTTTACACTCCAACTTCCTCAAAGGATGCTACAGGACCATCCTCAGCATTCAACAACCCAAAAGCCAATGCCTTATCTGCAAATTTTGGTATAAAGTCATGGATTCAAGCCCTTGGAAATCTGCCTCGTAATCAGATTGTATTTGGAATTCCTTTCCATGGTTGGGCTTGGAAGCTCTCCAACTCTCACCAACATGATGTGTTTTCTAAAGCGGATGGTGCAGCTCAAGGAGACTATATTTTAGAAGGTGGAGAAATTTACTACTCCAATGTGAAGAAGTTTATTGATGATAATCAAGCTGAAGTTGTGAACAATGACAAGAGGTTTCTCATTGCATACACTTATTTTGACACAACATGGATTGCATTTGAAAGTGAATACACAATTGCAGATAAGATTTCATTGGCAAAGTCCATTAAAGTGCTTGGTTACTTTGCTTCCAACATTGCAGCTGATGATGATGTTAACACTCTTGCTAATGCTGCTCTTGCTAATTGGTGA

Coding sequence (CDS)

ATGGATTCAAATATGTTAGCAGCTGCACCTCCCATCACTGATGTTGTGAAAGCTGGTTACTGGCTAGCAACCACTGACCCTATACTTCCTTCCGCCAATATCGATGCTATGCTTTTCACTCATCTCTTTGTTGGTTACGCTTCTGTTGACTCTACAACTTATAAAGTTACCATAGATTCTTCACAACTTGTTCTAATCAATCAGTTTACTTCAACTGTCACATTAAGTAATCCCGACGTCAAAACTTTGTTGTCTATTCAAGGATCCAATGACGTTTTTGCTCAAATTGCCAAGGATCCAACAAATCGTGATGTGTTCATCAAATCCTCCATTGAAGCAGCAAGAGCAGGTTCCTTTGATGGCCTTGATCTTCAATGGCTATATCCATCTTCAACTGAAGAAATGAAAAACTTTGAAACTTTTCTTGTTTTATGGCGCAAAGCTGTTGTGGAAGATGCCAAAAATTTGAGTGTTCCAGAGCTCCTCCTTGTTGCTTCAGTGTCCAACCTACCATATCTCACCCACAATATTCAATATCCAATTCAAACAATCAAACAAAAGTTAAATTGGGTGAATGTGATATCTTATGATTTTTACACTCCAACTTCCTCAAAGGATGCTACAGGACCATCCTCAGCATTCAACAACCCAAAAGCCAATGCCTTATCTGCAAATTTTGGTATAAAGTCATGGATTCAAGCCCTTGGAAATCTGCCTCGTAATCAGATTGTATTTGGAATTCCTTTCCATGGTTGGGCTTGGAAGCTCTCCAACTCTCACCAACATGATGTGTTTTCTAAAGCGGATGGTGCAGCTCAAGGAGACTATATTTTAGAAGGTGGAGAAATTTACTACTCCAATGTGAAGAAGTTTATTGATGATAATCAAGCTGAAGTTGTGAACAATGACAAGAGGTTTCTCATTGCATACACTTATTTTGACACAACATGGATTGCATTTGAAAGTGAATACACAATTGCAGATAAGATTTCATTGGCAAAGTCCATTAAAGTGCTTGGTTACTTTGCTTCCAACATTGCAGCTGATGATGATGTTAACACTCTTGCTAATGCTGCTCTTGCTAATTGGTGA

Protein sequence

MDSNMLAAAPPITDVVKAGYWLATTDPILPSANIDAMLFTHLFVGYASVDSTTYKVTIDSSQLVLINQFTSTVTLSNPDVKTLLSIQGSNDVFAQIAKDPTNRDVFIKSSIEAARAGSFDGLDLQWLYPSSTEEMKNFETFLVLWRKAVVEDAKNLSVPELLLVASVSNLPYLTHNIQYPIQTIKQKLNWVNVISYDFYTPTSSKDATGPSSAFNNPKANALSANFGIKSWIQALGNLPRNQIVFGIPFHGWAWKLSNSHQHDVFSKADGAAQGDYILEGGEIYYSNVKKFIDDNQAEVVNNDKRFLIAYTYFDTTWIAFESEYTIADKISLAKSIKVLGYFASNIAADDDVNTLANAALANW
Homology
BLAST of Lsi11G003740 vs. ExPASy Swiss-Prot
Match: O81862 (Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 9.2e-65
Identity = 148/354 (41.81%), Postives = 204/354 (57.63%), Query Frame = 0

Query: 15  VVKAGYWLATTDPILPSANIDAMLFTHLFVGYASVDSTTYKVTIDSSQLVLINQFTSTVT 74
           VVKA YW   ++   P  +ID+ LFTHLF  +A ++S T +VT+ S+     + FT TV 
Sbjct: 27  VVKASYWFPASE--FPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQ 86

Query: 75  LSNPDVKTLLSIQG---SNDVFAQIAKDPTNRDVFIKSSIEAARAGSFDGLDLQWLYPSS 134
             NP VKTLLSI G       +A +A +PT+R  FI SSI  AR+  F GLDL W YPSS
Sbjct: 87  RRNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSS 146

Query: 135 TEEMKNFETFLVLWRKAVVEDAKNLSVPELLLVASV--SNLPYLTHNIQYPIQTIKQKLN 194
             EM NF T L  WR AVV +A +   P LLL A+V  SN  Y   ++ YP+  +   L+
Sbjct: 147 ATEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSLD 206

Query: 195 WVNVISYDFYTPTSSKDATGPSSAFNNPKANALSANFGIKSWIQALGNLPRNQIVFGIPF 254
           WVN+++YDFY P  S+  TGP +A  +P     S + G +SWIQA   LP  + V G P+
Sbjct: 207 WVNLMAYDFYGPGWSR-VTGPPAALFDPSNAGPSGDAGTRSWIQA--GLPAKKAVLGFPY 266

Query: 255 HGWAWKLSNSHQHDVFSKADGAAQGDYILEGGEIYYSNVKKFIDDNQAEVVNNDKRFLIA 314
           +G+AW+L+N++ H  ++   GAA    I   G I Y  ++KFI DN A  V N    +  
Sbjct: 267 YGYAWRLTNANSHSYYAPTTGAA----ISPDGSIGYGQIRKFIVDNGATTVYN-STVVGD 326

Query: 315 YTYFDTTWIAFESEYTIADKISLAKSIKVLGYFASNIAADDDVNTLANAALANW 364
           Y Y  T WI ++   +I  K+  AK   +LGYF+ ++ ADD+ + L+ AA   W
Sbjct: 327 YCYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDN-SGLSRAASQAW 366

BLAST of Lsi11G003740 vs. ExPASy Swiss-Prot
Match: A0A072UR65 (Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 2.1e-48
Identity = 118/333 (35.44%), Postives = 183/333 (54.95%), Query Frame = 0

Query: 16  VKAGYWLATTDPILPSANIDAMLFTHLFVGYASVDSTTYKVTIDSSQLVLINQFTSTVTL 75
           VK  YWL   +P  P   +D  LFTH+F  + + ++ TYK+ I SSQ++ +N FT T   
Sbjct: 35  VKGIYWL--ENPFFPPTTVDTSLFTHIFYSFLTPNNITYKLEISSSQILSLNTFTKTFKT 94

Query: 76  SNPDVKTLLSIQG---SNDVFAQIAKDPTNRDVFIKSSIEAARAGSFDGLDLQWLYPSST 135
            +P   TL SI G   ++ + A IA DP     FI S+I+ AR   FDG+DL W +P +T
Sbjct: 95  KSPPAATLFSIGGAGSNSSLLAFIASDPPACAAFINSTIDVARTFGFDGIDLDWEFPKNT 154

Query: 136 EEMKNFETFLVLWRKAVVEDAKNLSVPELLLVASV---SNLPYLTHNIQYPIQTIKQKLN 195
           +EM +    L  WRKA+ ++      P LLL A+V    N         YP+ +I + L+
Sbjct: 155 KEMNDLGEMLFQWRKAISDEGATTGRPPLLLTAAVYFAVNFSIYGEPRMYPVNSINENLD 214

Query: 196 WVNVISYDFYTPTSSKDATGPSSAFNNPKANALSANFGIKSWIQALGNLPRNQIVFGIPF 255
           WVNV+SY+   P S+K  TG  S   +PK+N +S   G+ SWI + G +P  ++V G+P 
Sbjct: 215 WVNVMSYELRGPRSNK--TGAPSGTFDPKSN-VSVVSGLLSWIHS-GVVP-EKLVMGMPL 274

Query: 256 HGWAWKLSNSHQHDVFSKADGAAQGDYILEGGEIYYSNVKKFIDDNQAEVVNNDKRFLIA 315
           +G +WKL + + H + + + G+  G      G + Y  V  F     A+ V  D      
Sbjct: 275 YGKSWKLRDPNVHGIGAPSVGSGPG----VNGLMAYFQVLDFNRQKSAK-VEYDVDTASV 334

Query: 316 YTYFDTTWIAFESEYTIADKISLAKSIKVLGYF 343
           Y+Y  +TWI +++ +T++ K+  A+++K+ GYF
Sbjct: 335 YSYSGSTWIGYDNPFTVSIKVGFAQALKLRGYF 355

BLAST of Lsi11G003740 vs. ExPASy Swiss-Prot
Match: U5N4E3 (Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2)

HSP 1 Score: 190.3 bits (482), Expect = 3.9e-47
Identity = 126/369 (34.15%), Postives = 197/369 (53.39%), Query Frame = 0

Query: 3   SNMLAAAPPITDVVKAGYWLATTDPILPSANIDAMLFTHLFVGYASVDSTTYKVTIDSSQ 62
           S+    +P  T  VK  YW+   +P+ P ++ID  LFTH+F  + S +  TYK+  +   
Sbjct: 24  SSKSTPSPSSTTRVKGIYWI--ENPLFPPSSIDTSLFTHIFYAFVSPNKFTYKLEEEEED 83

Query: 63  LVLI----NQFTSTVTLSNPDVKTLLSIQG---SNDVFAQIAKDPTNRDVFIKSSIEAAR 122
              +      FT+T     P + TLLSI G   ++ +FA IA DPT R  FI S+I+ AR
Sbjct: 84  STTVATSLTTFTNTFKTKTPPIPTLLSIGGATSNSTLFAFIASDPTARATFINSTIQVAR 143

Query: 123 AGSFDGLDLQWLYPSSTEEMKNFETFLVLWRKAVVEDAKNLSVPELLLVASV---SNLPY 182
              FDG+D  W +P++T+EM +    L  WR+A+ ++  + S P LLL A+V    N   
Sbjct: 144 TFGFDGIDFDWEFPTTTKEMNDLGELLFQWRRAISDETASTSRPPLLLTAAVYFAVNFFL 203

Query: 183 LTHNIQYPIQTIKQKLNWVNVISYDFYTPTSSKDATGPSSAFNNPKANALSANFGIKSWI 242
                 YP+ +I + L+WVNV+SYD     S  + TG  S   + K+N +S   G+ SWI
Sbjct: 204 SGERRMYPVDSINKNLDWVNVMSYDL--RGSGSNVTGAPSGMFDSKSN-VSVVSGLFSWI 263

Query: 243 QALGNLPRNQIVFGIPFHGWAWKLSNSHQHDVFSKADGAAQGDYILEGGEIYYSNVKKFI 302
           +  G +   +IV G+P +G +WKL + + H + +   G   G   ++GG  Y+  V  F 
Sbjct: 264 R--GGVAPEKIVMGMPLYGKSWKLQDPNVHGIGAPNVGPGPG---VDGGMAYF-QVVDFN 323

Query: 303 DDNQAEVVNNDKRFLIAYTYFDTTWIAFESEYTIADKISLAKSIKVLGYF---ASNIAAD 359
               A+VV  DK     Y+Y  +TWI ++  +T++ K+  A+++K+ GYF   A    +D
Sbjct: 324 KQMGAKVV-YDKETGSVYSYSGSTWIGYDDPFTVSVKVGFAQALKLGGYFFWAAGYDTSD 380

BLAST of Lsi11G003740 vs. ExPASy Swiss-Prot
Match: A0A1B1J8Z2 (Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2)

HSP 1 Score: 158.3 bits (399), Expect = 1.6e-37
Identity = 111/361 (30.75%), Postives = 187/361 (51.80%), Query Frame = 0

Query: 10  PPITDVVKAGYWLATTDPILPSANIDAMLFTHLFVGYASVDSTTYKVTIDSSQLVLINQF 69
           PP    V+A YW A +D    +++ID   FTH++  +      ++ + I  S      ++
Sbjct: 49  PPSKGGVRAAYWPAWSD--FSTSSIDTNYFTHIYYAFVQPAPESFNLEITESYKKWAPKY 108

Query: 70  TSTVTLSNPDVKTLLSIQGSND---VFAQIAKDPTNRDVFIKSSIEAARAGSFDGLDLQW 129
                +  P V TLLSI G  +   +F+++A    NR  FI S+I  AR   F+GLDL W
Sbjct: 109 DGIHNI-RPRVTTLLSIGGGGNNATLFSEMASSKQNRASFINSTIHVARKHEFNGLDLDW 168

Query: 130 LYPSSTEEMKNFETFLVLWRKAVVEDAKNLSVPELLLVASV---SNLPYLTHNIQ-YPIQ 189
            +P   ++M N    L  W KA+V +A       LLL ++V   S +  + +  + YP++
Sbjct: 169 EWPGDEKDMSNLALLLKEWYKALVVEANTSRKSRLLLTSAVYFNSTISLIGNGPRSYPVR 228

Query: 190 TIKQKLNWVNVISYDFYTPTSSKDATGPSSAFNNPKANALSANFGIKSWIQALGNLPRNQ 249
            I++ L+W + + +D+    +++  TG ++A  +P +N +S  +GI SWI +   +P  +
Sbjct: 229 AIRKYLDWASPMCFDYNGAWANE--TGFNAALYDPNSN-ISTKYGIGSWIGS--GVPAEK 288

Query: 250 IVFGIPFHGWAWKLSNSHQHDVFSKADGAAQGDYILEGGEIYYSNVKKFIDDNQAEVVNN 309
           +V G+P +G AW+L + + H V +KA G A    +   G + Y  +  F  D  A+VV  
Sbjct: 289 LVMGLPLYGRAWELKDPNDHGVGAKAVGPA----VDTDGSMDYDEILVFNKDTGAKVV-Y 348

Query: 310 DKRFLIAYTYFDTTWIAFESEYTIADKISLAKSIKVLGYFASNIAADDDVNTLANAALAN 364
           D+  +  Y+Y  TTWI ++   +I  K+  A+S+ + GYF   I  D D  T++  A   
Sbjct: 349 DEVAVSFYSYSGTTWIGYDDGPSITKKVQFARSMGLKGYFFWAIGKDKDW-TISKQASNA 395

BLAST of Lsi11G003740 vs. ExPASy Swiss-Prot
Match: A0A072VEP0 (Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.8e-36
Identity = 108/348 (31.03%), Postives = 179/348 (51.44%), Query Frame = 0

Query: 16  VKAGYWLATTDPILPSANIDAMLFTHLFVGYASVDSTTYKVTIDSSQLVLINQFTSTVTL 75
           V++ YW A  D   PS  ID   FTH+ + +   +  ++K+ I  S +     F   +  
Sbjct: 40  VRSAYWPA-GDDFSPSL-IDTNYFTHILLAFIQPEPISFKLEITKSGIKWGQNFIKALRH 99

Query: 76  SNPDVKTLLSIQG---SNDVFAQIAKDPTNRDVFIKSSIEAARAGSFDGLDLQWLYPSST 135
            +P VKTLLSI G   ++ +F++IA    NR++FI S+IE AR   FDG+DL W +P + 
Sbjct: 100 RSPPVKTLLSIGGGGSNSTLFSEIASTKQNREIFINSTIEVARKYRFDGVDLDWEFPETQ 159

Query: 136 EEMKNFETFLVLWRKAVVEDAKNLSVPELLLVASVSNLPYLTHNIQ---------YPIQT 195
           ++M N       W  A+  +AK    P LLL ++V    Y    ++         YP Q 
Sbjct: 160 QDMFNLGLLYEEWYNALFAEAKVRRKPRLLLTSAV----YYNSTVRLIGKHGPRSYPTQA 219

Query: 196 IKQKLNWVNVISYDFYTPTSSKDATGPSSAFNNPKANALSANFGIKSWIQALGNLPRNQI 255
           I + L+W + + +D++    +   T  ++A  + K+  +S NFG+ SWI++   +   ++
Sbjct: 220 INKYLDWASPMCFDYHGTWDNN--TDFNAALYDSKSE-ISTNFGLHSWIKS--GVRPEKL 279

Query: 256 VFGIPFHGWAWKLSNSHQHDVFSKADGAAQGDYILEGGEIYYSNVKKFIDDNQAEVVNND 315
           V G+  +G AW+L + + + V ++A G A        G + Y+ + KF   + A VV  D
Sbjct: 280 VMGLALYGRAWELKDPNVNGVGAEAVGPATD----TDGSMNYNEILKFNKQSGANVV-YD 339

Query: 316 KRFLIAYTYFDTTWIAFESEYTIADKISLAKSIKVLGYFASNIAADDD 352
           K  +  Y+Y  TTWI ++   +I  K+  AKS+ + GYF   +  D D
Sbjct: 340 KVAISFYSYAGTTWIGYDDGPSITTKVRFAKSLGLKGYFFWALGKDKD 371

BLAST of Lsi11G003740 vs. ExPASy TrEMBL
Match: A0A6J1KB82 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 3.1e-140
Identity = 246/352 (69.89%), Postives = 296/352 (84.09%), Query Frame = 0

Query: 12  ITDVVKAGYWLATTDPILPSANIDAMLFTHLFVGYASVDSTTYKVTIDSSQLVLINQFTS 71
           +T+ V AGYWLA+TD  LP+ANIDA LFTHLF GYA ++S TY+VT+D SQL+LI+QF+S
Sbjct: 7   VTEFVNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILIDQFSS 66

Query: 72  TVTLSNPDVKTLLSIQGSNDVFAQIAKDPTNRDVFIKSSIEAARAGSFDGLDLQWLYPSS 131
           TV LSNPDVKTLLSI G   VFA +A DPTNRD FIKSSI AAR G FDGLDLQWLYPSS
Sbjct: 67  TVKLSNPDVKTLLSIAGDAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSS 126

Query: 132 TEEMKNFETFLVLWRKAVVEDAKNLSVPELLLVASVSNLPYLTHNIQYPIQTIKQKLNWV 191
            ++M NF+T +  W +AVV+DA ++S+P+LLLVA+VSNLPYL +NIQYP   I QKL+WV
Sbjct: 127 QDQMANFKTLIHSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQKLDWV 186

Query: 192 NVISYDFYTPTSSKDATGPSSAFNNPKANALSANFGIKSWIQALGNLPRNQIVFGIPFHG 251
           N++SYDFYTP S + ATGPSSAF+NPKANALSANFGI+SW Q+L NL   +IVFGIPFHG
Sbjct: 187 NLLSYDFYTPDSFRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLSHKKIVFGIPFHG 246

Query: 252 WAWKLSNSHQHDVFSKADGAAQGDYILEGGEIYYSNVKKFIDDNQAEVVNNDKRFLIAYT 311
           WAWKL+NSHQH +F+KADGAA G+YI   G+I+YS+V+KFI+DNQA V + DK +L+AY 
Sbjct: 247 WAWKLANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYA 306

Query: 312 YFDTTWIAFESEYTIADKISLAKSIKVLGYFASNIAADDDVNTLANAALANW 364
           Y++TTWI FESEYT+ADKI+LAK+ KVLGYFA NIAADD  NTLANAA +NW
Sbjct: 307 YYETTWITFESEYTVADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Lsi11G003740 vs. ExPASy TrEMBL
Match: A0A6J1G2P4 (acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE=4 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 4.1e-140
Identity = 247/352 (70.17%), Postives = 295/352 (83.81%), Query Frame = 0

Query: 12  ITDVVKAGYWLATTDPILPSANIDAMLFTHLFVGYASVDSTTYKVTIDSSQLVLINQFTS 71
           +T+ V AGYWLA+TD  LP+ANIDA LFTHLF GYA ++S TY+VT+D SQL+LI+QF+S
Sbjct: 7   VTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSGTYQVTVDPSQLILIDQFSS 66

Query: 72  TVTLSNPDVKTLLSIQGSNDVFAQIAKDPTNRDVFIKSSIEAARAGSFDGLDLQWLYPSS 131
           TV LSNP+VKTLLSI     VFA +A DPTNRD FIKSSI AAR G FDGLDLQWLYPSS
Sbjct: 67  TVKLSNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSS 126

Query: 132 TEEMKNFETFLVLWRKAVVEDAKNLSVPELLLVASVSNLPYLTHNIQYPIQTIKQKLNWV 191
            ++M NF+T +  W +AVV+DA +LS+P+LLLVA+VSNLPYL  NIQYP   I QKL+WV
Sbjct: 127 QDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWV 186

Query: 192 NVISYDFYTPTSSKDATGPSSAFNNPKANALSANFGIKSWIQALGNLPRNQIVFGIPFHG 251
           N++SYDFYTP SS+ ATGPSSAF+NPKANALSANFGI+SW Q+L NLP  +IVFGIPFHG
Sbjct: 187 NLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHG 246

Query: 252 WAWKLSNSHQHDVFSKADGAAQGDYILEGGEIYYSNVKKFIDDNQAEVVNNDKRFLIAYT 311
           WAWKL NSHQH +F+KADGAA G+YI   G+I+YS+V+KFI+DNQA V + DK +L+AY 
Sbjct: 247 WAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYA 306

Query: 312 YFDTTWIAFESEYTIADKISLAKSIKVLGYFASNIAADDDVNTLANAALANW 364
           Y++TTWI +ESEYTIADKI+LAK+ KVLGYFA NIAADD  NTLANAA +NW
Sbjct: 307 YYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Lsi11G003740 vs. ExPASy TrEMBL
Match: A0A6J1KFT3 (chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493907 PE=4 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 5.3e-140
Identity = 245/352 (69.60%), Postives = 295/352 (83.81%), Query Frame = 0

Query: 12  ITDVVKAGYWLATTDPILPSANIDAMLFTHLFVGYASVDSTTYKVTIDSSQLVLINQFTS 71
           +T+ V AGYWLA+TD  LP+ANIDA LFTHLF GYA ++S TY+VT+D SQL+LI+QF+S
Sbjct: 7   VTEFVNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILIDQFSS 66

Query: 72  TVTLSNPDVKTLLSIQGSNDVFAQIAKDPTNRDVFIKSSIEAARAGSFDGLDLQWLYPSS 131
           TV LSNP VKTLLSI G   VFA +A DPTNRD FIKSSI AAR G FDGLDLQWLYPSS
Sbjct: 67  TVKLSNPGVKTLLSIAGDAAVFAAMAVDPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSS 126

Query: 132 TEEMKNFETFLVLWRKAVVEDAKNLSVPELLLVASVSNLPYLTHNIQYPIQTIKQKLNWV 191
            ++M NF+T +  W +AVV+DA ++S+P+LLLVA+VSNLPYL +NIQYP   I Q L+WV
Sbjct: 127 QDQMANFKTLIQSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQNLDWV 186

Query: 192 NVISYDFYTPTSSKDATGPSSAFNNPKANALSANFGIKSWIQALGNLPRNQIVFGIPFHG 251
           N++SYDFYTP S +DATGPSSAF+NPKANALSANFGI+SW Q+L NL   +IVFGIPFHG
Sbjct: 187 NLLSYDFYTPDSFRDATGPSSAFHNPKANALSANFGIRSWAQSLNNLSHKKIVFGIPFHG 246

Query: 252 WAWKLSNSHQHDVFSKADGAAQGDYILEGGEIYYSNVKKFIDDNQAEVVNNDKRFLIAYT 311
           WAWKL+NSHQH +F+KADGAA G+YI   G+I+YS+V+KFI+DNQA V + DK +L+AY 
Sbjct: 247 WAWKLANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYA 306

Query: 312 YFDTTWIAFESEYTIADKISLAKSIKVLGYFASNIAADDDVNTLANAALANW 364
           Y++TTWI FESEYT+ADKI+LAK+ KVLGYFA NIAADD  NTLANAA +NW
Sbjct: 307 YYETTWITFESEYTVADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Lsi11G003740 vs. ExPASy TrEMBL
Match: A0A6J1G4H5 (chitotriosidase-1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450666 PE=4 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 1.2e-136
Identity = 245/352 (69.60%), Postives = 289/352 (82.10%), Query Frame = 0

Query: 12  ITDVVKAGYWLATTDPILPSANIDAMLFTHLFVGYASVDSTTYKVTIDSSQLVLINQFTS 71
           +T  V AGYWLA+T+  L +ANIDA LFTHLFVGYA +D  T++VT+D SQL+LINQF+S
Sbjct: 7   VTKFVNAGYWLASTNDRLLAANIDATLFTHLFVGYALLDPKTHQVTVDPSQLILINQFSS 66

Query: 72  TVTLSNPDVKTLLSIQGSNDVFAQIAKDPTNRDVFIKSSIEAARAGSFDGLDLQWLYPSS 131
           TV LSNPDVKTLLSI     VFA +A  PTNRD FIKSSI AAR G FDGLDLQWLYPSS
Sbjct: 67  TVKLSNPDVKTLLSIAADAAVFAAMAASPTNRDDFIKSSIAAARGGGFDGLDLQWLYPSS 126

Query: 132 TEEMKNFETFLVLWRKAVVEDAKNLSVPELLLVASVSNLPYLTHNIQYPIQTIKQKLNWV 191
             EM NF+T +  W +AVV+DA  +S+P+LLLVA+VSNLPYL  N++YP   I QKL+WV
Sbjct: 127 QYEMGNFKTLIHSWHQAVVDDATRVSLPQLLLVATVSNLPYLQKNVEYPADAINQKLDWV 186

Query: 192 NVISYDFYTPTSSKDATGPSSAFNNPKANALSANFGIKSWIQALGNLPRNQIVFGIPFHG 251
           N++SYDFYTP SSK ATGPSSAF+NPKANALSANFGI+SW Q+L NLP  +IVFGIPFHG
Sbjct: 187 NLLSYDFYTPDSSKAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHG 246

Query: 252 WAWKLSNSHQHDVFSKADGAAQGDYILEGGEIYYSNVKKFIDDNQAEVVNNDKRFLIAYT 311
           WAWKL NSHQH  F+KADGAA G+YI   G+I+YS+V+KFI+DNQA V + DK +L+AY 
Sbjct: 247 WAWKLFNSHQHAPFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYA 306

Query: 312 YFDTTWIAFESEYTIADKISLAKSIKVLGYFASNIAADDDVNTLANAALANW 364
           Y++TTWI +ESEYTIADKI+LAK+ KVLGYFA NIAADD  NTLANAA +NW
Sbjct: 307 YYETTWITYESEYTIADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Lsi11G003740 vs. ExPASy TrEMBL
Match: A0A6J1G4J9 (acidic mammalian chitinase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450666 PE=4 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 1.2e-136
Identity = 245/352 (69.60%), Postives = 288/352 (81.82%), Query Frame = 0

Query: 12  ITDVVKAGYWLATTDPILPSANIDAMLFTHLFVGYASVDSTTYKVTIDSSQLVLINQFTS 71
           + + V AGYWLA TD  L +ANIDA LFTHLFVGYA +D  T++VT+D SQL+LINQF+S
Sbjct: 1   MAEFVNAGYWLAGTDDTLLAANIDATLFTHLFVGYALLDPKTHQVTVDPSQLILINQFSS 60

Query: 72  TVTLSNPDVKTLLSIQGSNDVFAQIAKDPTNRDVFIKSSIEAARAGSFDGLDLQWLYPSS 131
           TV LSNPDVKTLLSI     VFA +A  PTNRD FIKSSI AAR G FDGLDLQWLYPSS
Sbjct: 61  TVKLSNPDVKTLLSIAADAAVFAAMAASPTNRDDFIKSSIAAARGGGFDGLDLQWLYPSS 120

Query: 132 TEEMKNFETFLVLWRKAVVEDAKNLSVPELLLVASVSNLPYLTHNIQYPIQTIKQKLNWV 191
             EM NF+T +  W +AVV+DA  +S+P+LLLVA+VSNLPYL  N++YP   I QKL+WV
Sbjct: 121 QYEMGNFKTLIHSWHQAVVDDATRVSLPQLLLVATVSNLPYLQKNVEYPADAINQKLDWV 180

Query: 192 NVISYDFYTPTSSKDATGPSSAFNNPKANALSANFGIKSWIQALGNLPRNQIVFGIPFHG 251
           N++SYDFYTP SSK ATGPSSAF+NPKANALSANFGI+SW Q+L NLP  +IVFGIPFHG
Sbjct: 181 NLLSYDFYTPDSSKAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHG 240

Query: 252 WAWKLSNSHQHDVFSKADGAAQGDYILEGGEIYYSNVKKFIDDNQAEVVNNDKRFLIAYT 311
           WAWKL NSHQH  F+KADGAA G+YI   G+I+YS+V+KFI+DNQA V + DK +L+AY 
Sbjct: 241 WAWKLFNSHQHAPFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYA 300

Query: 312 YFDTTWIAFESEYTIADKISLAKSIKVLGYFASNIAADDDVNTLANAALANW 364
           Y++TTWI +ESEYTIADKI+LAK+ KVLGYFA NIAADD  NTLANAA +NW
Sbjct: 301 YYETTWITYESEYTIADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 352

BLAST of Lsi11G003740 vs. NCBI nr
Match: XP_023521369.1 (acidic mammalian chitinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 511.9 bits (1317), Expect = 4.5e-141
Identity = 250/352 (71.02%), Postives = 295/352 (83.81%), Query Frame = 0

Query: 12  ITDVVKAGYWLATTDPILPSANIDAMLFTHLFVGYASVDSTTYKVTIDSSQLVLINQFTS 71
           +T+ V AGYWLA+TD  LP+ANIDA LFTHLF GYA ++S TY+VTID SQL+L+NQF+ 
Sbjct: 13  VTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLILLNQFSP 72

Query: 72  TVTLSNPDVKTLLSIQGSNDVFAQIAKDPTNRDVFIKSSIEAARAGSFDGLDLQWLYPSS 131
           TV LSNPDVKTLLSI     VFA +A DPTNRD FIKSSI AAR G FDGLDLQWLYPSS
Sbjct: 73  TVRLSNPDVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSS 132

Query: 132 TEEMKNFETFLVLWRKAVVEDAKNLSVPELLLVASVSNLPYLTHNIQYPIQTIKQKLNWV 191
            EEM NF+T +  W +AVV+DA +LS+P+LLLVA+VSNLPYL  NIQYP   I QKL+WV
Sbjct: 133 QEEMGNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWV 192

Query: 192 NVISYDFYTPTSSKDATGPSSAFNNPKANALSANFGIKSWIQALGNLPRNQIVFGIPFHG 251
           N++SYDFYTP S++ ATGPSSAF+NPKANALSANFGI+SW Q+L NLP  +IVFGIPFHG
Sbjct: 193 NLLSYDFYTPDSNRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHG 252

Query: 252 WAWKLSNSHQHDVFSKADGAAQGDYILEGGEIYYSNVKKFIDDNQAEVVNNDKRFLIAYT 311
           WAWKL NSHQH +F+KADGAA G+YI   G+I+YS+V+KFI+DNQA V + DK +L+AYT
Sbjct: 253 WAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYT 312

Query: 312 YFDTTWIAFESEYTIADKISLAKSIKVLGYFASNIAADDDVNTLANAALANW 364
           YF+TTWI +ES+YTIADKI+LAK+ KVLGYFA NIAADD  NTLANAA +NW
Sbjct: 313 YFETTWITYESDYTIADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 364

BLAST of Lsi11G003740 vs. NCBI nr
Match: XP_023545402.1 (acidic mammalian chitinase-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 508.8 bits (1309), Expect = 3.8e-140
Identity = 249/348 (71.55%), Postives = 292/348 (83.91%), Query Frame = 0

Query: 16  VKAGYWLATTDPILPSANIDAMLFTHLFVGYASVDSTTYKVTIDSSQLVLINQFTSTVTL 75
           V AGYWLA+TD  LP+ANIDA LFTHLF GYA ++S TY+VTID SQL+L+NQF+ TV L
Sbjct: 2   VNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSDTYQVTIDPSQLILLNQFSPTVRL 61

Query: 76  SNPDVKTLLSIQGSNDVFAQIAKDPTNRDVFIKSSIEAARAGSFDGLDLQWLYPSSTEEM 135
           SNPDVKTLLSI     VFA +A DPTNRD FIKSSI AAR G FDGLDLQWLYPSS EEM
Sbjct: 62  SNPDVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSSQEEM 121

Query: 136 KNFETFLVLWRKAVVEDAKNLSVPELLLVASVSNLPYLTHNIQYPIQTIKQKLNWVNVIS 195
            NF+T +  W +AVV+DA +LS+P+LLLVA+VSNLPYL  NIQYP   I QKL+WVN++S
Sbjct: 122 GNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWVNLLS 181

Query: 196 YDFYTPTSSKDATGPSSAFNNPKANALSANFGIKSWIQALGNLPRNQIVFGIPFHGWAWK 255
           YDFYTP S++ ATGPSSAF+NPKANALSANFGI+SW Q+L NLP  +IVFGIPFHGWAWK
Sbjct: 182 YDFYTPDSNRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHGWAWK 241

Query: 256 LSNSHQHDVFSKADGAAQGDYILEGGEIYYSNVKKFIDDNQAEVVNNDKRFLIAYTYFDT 315
           L NSHQH +F+KADGAA G+YI   G+I+YS+V+KFI+DNQA V + DK +L+AYTYF+T
Sbjct: 242 LFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYTYFET 301

Query: 316 TWIAFESEYTIADKISLAKSIKVLGYFASNIAADDDVNTLANAALANW 364
           TWI +ES+YTIADKI+LAK+ KVLGYFA NIAADD  NTLANAA +NW
Sbjct: 302 TWITYESDYTIADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 349

BLAST of Lsi11G003740 vs. NCBI nr
Match: XP_022999577.1 (chitotriosidase-1-like [Cucurbita maxima])

HSP 1 Score: 508.1 bits (1307), Expect = 6.4e-140
Identity = 246/352 (69.89%), Postives = 296/352 (84.09%), Query Frame = 0

Query: 12  ITDVVKAGYWLATTDPILPSANIDAMLFTHLFVGYASVDSTTYKVTIDSSQLVLINQFTS 71
           +T+ V AGYWLA+TD  LP+ANIDA LFTHLF GYA ++S TY+VT+D SQL+LI+QF+S
Sbjct: 7   VTEFVNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILIDQFSS 66

Query: 72  TVTLSNPDVKTLLSIQGSNDVFAQIAKDPTNRDVFIKSSIEAARAGSFDGLDLQWLYPSS 131
           TV LSNPDVKTLLSI G   VFA +A DPTNRD FIKSSI AAR G FDGLDLQWLYPSS
Sbjct: 67  TVKLSNPDVKTLLSIAGDAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSS 126

Query: 132 TEEMKNFETFLVLWRKAVVEDAKNLSVPELLLVASVSNLPYLTHNIQYPIQTIKQKLNWV 191
            ++M NF+T +  W +AVV+DA ++S+P+LLLVA+VSNLPYL +NIQYP   I QKL+WV
Sbjct: 127 QDQMANFKTLIHSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQKLDWV 186

Query: 192 NVISYDFYTPTSSKDATGPSSAFNNPKANALSANFGIKSWIQALGNLPRNQIVFGIPFHG 251
           N++SYDFYTP S + ATGPSSAF+NPKANALSANFGI+SW Q+L NL   +IVFGIPFHG
Sbjct: 187 NLLSYDFYTPDSFRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLSHKKIVFGIPFHG 246

Query: 252 WAWKLSNSHQHDVFSKADGAAQGDYILEGGEIYYSNVKKFIDDNQAEVVNNDKRFLIAYT 311
           WAWKL+NSHQH +F+KADGAA G+YI   G+I+YS+V+KFI+DNQA V + DK +L+AY 
Sbjct: 247 WAWKLANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYA 306

Query: 312 YFDTTWIAFESEYTIADKISLAKSIKVLGYFASNIAADDDVNTLANAALANW 364
           Y++TTWI FESEYT+ADKI+LAK+ KVLGYFA NIAADD  NTLANAA +NW
Sbjct: 307 YYETTWITFESEYTVADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Lsi11G003740 vs. NCBI nr
Match: XP_022946077.1 (acidic mammalian chitinase-like [Cucurbita moschata] >XP_022946671.1 acidic mammalian chitinase-like [Cucurbita moschata])

HSP 1 Score: 507.7 bits (1306), Expect = 8.4e-140
Identity = 247/352 (70.17%), Postives = 295/352 (83.81%), Query Frame = 0

Query: 12  ITDVVKAGYWLATTDPILPSANIDAMLFTHLFVGYASVDSTTYKVTIDSSQLVLINQFTS 71
           +T+ V AGYWLA+TD  LP+ANIDA LFTHLF GYA ++S TY+VT+D SQL+LI+QF+S
Sbjct: 7   VTEFVNAGYWLASTDDRLPAANIDATLFTHLFAGYAQLNSGTYQVTVDPSQLILIDQFSS 66

Query: 72  TVTLSNPDVKTLLSIQGSNDVFAQIAKDPTNRDVFIKSSIEAARAGSFDGLDLQWLYPSS 131
           TV LSNP+VKTLLSI     VFA +A DPTNRD FIKSSI AAR G FDGLDLQWLYPSS
Sbjct: 67  TVKLSNPEVKTLLSIAADAAVFAAMAADPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSS 126

Query: 132 TEEMKNFETFLVLWRKAVVEDAKNLSVPELLLVASVSNLPYLTHNIQYPIQTIKQKLNWV 191
            ++M NF+T +  W +AVV+DA +LS+P+LLLVA+VSNLPYL  NIQYP   I QKL+WV
Sbjct: 127 QDQMDNFKTLIHSWHQAVVDDATSLSLPQLLLVATVSNLPYLQSNIQYPADAINQKLDWV 186

Query: 192 NVISYDFYTPTSSKDATGPSSAFNNPKANALSANFGIKSWIQALGNLPRNQIVFGIPFHG 251
           N++SYDFYTP SS+ ATGPSSAF+NPKANALSANFGI+SW Q+L NLP  +IVFGIPFHG
Sbjct: 187 NLLSYDFYTPDSSRAATGPSSAFHNPKANALSANFGIRSWAQSLHNLPHKKIVFGIPFHG 246

Query: 252 WAWKLSNSHQHDVFSKADGAAQGDYILEGGEIYYSNVKKFIDDNQAEVVNNDKRFLIAYT 311
           WAWKL NSHQH +F+KADGAA G+YI   G+I+YS+V+KFI+DNQA V + DK +L+AY 
Sbjct: 247 WAWKLFNSHQHTLFAKADGAATGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYA 306

Query: 312 YFDTTWIAFESEYTIADKISLAKSIKVLGYFASNIAADDDVNTLANAALANW 364
           Y++TTWI +ESEYTIADKI+LAK+ KVLGYFA NIAADD  NTLANAA +NW
Sbjct: 307 YYETTWITYESEYTIADKIALAKTDKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Lsi11G003740 vs. NCBI nr
Match: XP_022999580.1 (chitotriosidase-1-like [Cucurbita maxima])

HSP 1 Score: 507.3 bits (1305), Expect = 1.1e-139
Identity = 245/352 (69.60%), Postives = 295/352 (83.81%), Query Frame = 0

Query: 12  ITDVVKAGYWLATTDPILPSANIDAMLFTHLFVGYASVDSTTYKVTIDSSQLVLINQFTS 71
           +T+ V AGYWLA+TD  LP+ANIDA LFTHLF GYA ++S TY+VT+D SQL+LI+QF+S
Sbjct: 7   VTEFVNAGYWLASTDGTLPAANIDATLFTHLFAGYAQLNSDTYQVTVDPSQLILIDQFSS 66

Query: 72  TVTLSNPDVKTLLSIQGSNDVFAQIAKDPTNRDVFIKSSIEAARAGSFDGLDLQWLYPSS 131
           TV LSNP VKTLLSI G   VFA +A DPTNRD FIKSSI AAR G FDGLDLQWLYPSS
Sbjct: 67  TVKLSNPGVKTLLSIAGDAAVFAAMAVDPTNRDAFIKSSIAAARGGGFDGLDLQWLYPSS 126

Query: 132 TEEMKNFETFLVLWRKAVVEDAKNLSVPELLLVASVSNLPYLTHNIQYPIQTIKQKLNWV 191
            ++M NF+T +  W +AVV+DA ++S+P+LLLVA+VSNLPYL +NIQYP   I Q L+WV
Sbjct: 127 QDQMANFKTLIQSWHQAVVDDATSVSLPQLLLVATVSNLPYLQNNIQYPADAITQNLDWV 186

Query: 192 NVISYDFYTPTSSKDATGPSSAFNNPKANALSANFGIKSWIQALGNLPRNQIVFGIPFHG 251
           N++SYDFYTP S +DATGPSSAF+NPKANALSANFGI+SW Q+L NL   +IVFGIPFHG
Sbjct: 187 NLLSYDFYTPDSFRDATGPSSAFHNPKANALSANFGIRSWAQSLNNLSHKKIVFGIPFHG 246

Query: 252 WAWKLSNSHQHDVFSKADGAAQGDYILEGGEIYYSNVKKFIDDNQAEVVNNDKRFLIAYT 311
           WAWKL+NSHQH +F+KADGAA G+YI   G+I+YS+V+KFI+DNQA V + DK +L+AY 
Sbjct: 247 WAWKLANSHQHALFAKADGAASGEYIGPHGQIFYSDVQKFIEDNQAGVEDKDKSYLVAYA 306

Query: 312 YFDTTWIAFESEYTIADKISLAKSIKVLGYFASNIAADDDVNTLANAALANW 364
           Y++TTWI FESEYT+ADKI+LAK+ KVLGYFA NIAADD  NTLANAA +NW
Sbjct: 307 YYETTWITFESEYTVADKIALAKADKVLGYFAFNIAADDASNTLANAAKSNW 358

BLAST of Lsi11G003740 vs. TAIR 10
Match: AT4G19810.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 248.8 bits (634), Expect = 6.6e-66
Identity = 148/354 (41.81%), Postives = 204/354 (57.63%), Query Frame = 0

Query: 15  VVKAGYWLATTDPILPSANIDAMLFTHLFVGYASVDSTTYKVTIDSSQLVLINQFTSTVT 74
           VVKA YW   ++   P  +ID+ LFTHLF  +A ++S T +VT+ S+     + FT TV 
Sbjct: 27  VVKASYWFPASE--FPVTDIDSSLFTHLFCAFADLNSQTNQVTVSSANQPKFSTFTQTVQ 86

Query: 75  LSNPDVKTLLSIQG---SNDVFAQIAKDPTNRDVFIKSSIEAARAGSFDGLDLQWLYPSS 134
             NP VKTLLSI G       +A +A +PT+R  FI SSI  AR+  F GLDL W YPSS
Sbjct: 87  RRNPSVKTLLSIGGGIADKTAYASMASNPTSRKSFIDSSIRVARSYGFHGLDLDWEYPSS 146

Query: 135 TEEMKNFETFLVLWRKAVVEDAKNLSVPELLLVASV--SNLPYLTHNIQYPIQTIKQKLN 194
             EM NF T L  WR AVV +A +   P LLL A+V  SN  Y   ++ YP+  +   L+
Sbjct: 147 ATEMTNFGTLLREWRSAVVAEASSSGKPRLLLAAAVFYSNNYY---SVLYPVSAVASSLD 206

Query: 195 WVNVISYDFYTPTSSKDATGPSSAFNNPKANALSANFGIKSWIQALGNLPRNQIVFGIPF 254
           WVN+++YDFY P  S+  TGP +A  +P     S + G +SWIQA   LP  + V G P+
Sbjct: 207 WVNLMAYDFYGPGWSR-VTGPPAALFDPSNAGPSGDAGTRSWIQA--GLPAKKAVLGFPY 266

Query: 255 HGWAWKLSNSHQHDVFSKADGAAQGDYILEGGEIYYSNVKKFIDDNQAEVVNNDKRFLIA 314
           +G+AW+L+N++ H  ++   GAA    I   G I Y  ++KFI DN A  V N    +  
Sbjct: 267 YGYAWRLTNANSHSYYAPTTGAA----ISPDGSIGYGQIRKFIVDNGATTVYN-STVVGD 326

Query: 315 YTYFDTTWIAFESEYTIADKISLAKSIKVLGYFASNIAADDDVNTLANAALANW 364
           Y Y  T WI ++   +I  K+  AK   +LGYF+ ++ ADD+ + L+ AA   W
Sbjct: 327 YCYAGTNWIGYDDNQSIVTKVRYAKQRGLLGYFSWHVGADDN-SGLSRAASQAW 366

BLAST of Lsi11G003740 vs. TAIR 10
Match: AT4G19820.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 236.9 bits (603), Expect = 2.6e-62
Identity = 135/340 (39.71%), Postives = 197/340 (57.94%), Query Frame = 0

Query: 15  VVKAGYWLATTDPILPSANIDAMLFTHLFVGYASVDSTTYKVTIDSSQLVLINQFTSTVT 74
           VVKA YW A ++   P A ID+ LFTHLF  +A +++ TY+V + S      + FT TV 
Sbjct: 25  VVKATYWFAESES--PLAQIDSSLFTHLFCAFADINTLTYQVIVSSRNKPKFSTFTQTVR 84

Query: 75  LSNPDVKTLLSIQGS---NDVFAQIAKDPTNRDVFIKSSIEAARAGSFDGLDLQWLYPSS 134
             NP VKTLLSI G    N  FA +A +PT+R +FI SSI+ AR+  F GLDL W YPS 
Sbjct: 85  RRNPTVKTLLSIGGDFTYNFAFASMASNPTSRKLFISSSIKLARSCGFHGLDLNWKYPSI 144

Query: 135 TEEMKNFETFLVLWRKAVVEDAKNLSVPELLLVASVSNLPYLTHNIQYPIQTIKQKLNWV 194
           T EM NF   L  WR AV  +A++   P LLL A+V    Y  +++ +P+  +   L+WV
Sbjct: 145 TTEMDNFGKLLREWRLAVEAEARSSGKPRLLLTAAVF-YSYSYYSVLHPVNAVADSLDWV 204

Query: 195 NVISYDFYTPTSSKDATGPSSAFNNPKANALSANFGIKSWIQALGNLPRNQIVFGIPFHG 254
           N+++YDFY   SS+    P+  + +P     S + G+++W QA   LP  + V G P +G
Sbjct: 205 NLVAYDFYESGSSRVTCSPAPLY-DPITTGPSGDAGVRAWTQA--GLPAKKAVLGFPLYG 264

Query: 255 WAWKLSNSHQHDVFSKADGAAQGDYILEGGEIYYSNVKKFIDDNQAEVVNNDKRFLIAYT 314
           +AW L+++  H+ ++ + G A    I   G I Y  +++FI DN+A +V N    +  Y 
Sbjct: 265 YAWCLTDAKNHNYYANSSGPA----ISPDGSIGYDQIRRFIVDNKATMVYN-SNLVQNYC 324

Query: 315 YFDTTWIAFESEYTIADKISLAKSIKVLGYFASNIAADDD 352
           Y   TWI ++   +I  K+  AK   +LGYF+ +I ADD+
Sbjct: 325 YAKKTWIGYDDNQSIVMKVKYAKQRGLLGYFSWHIGADDN 353

BLAST of Lsi11G003740 vs. TAIR 10
Match: AT4G19800.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 224.9 bits (572), Expect = 1.0e-58
Identity = 136/345 (39.42%), Postives = 198/345 (57.39%), Query Frame = 0

Query: 13  TDVVKAGYWLATTDPILPSANIDAMLFTHLFVGYASVDSTTYKVTIDSSQLVLINQFTST 72
           +DVVKA YW   TD   P+ +ID+ LFTHLF  +A +++ +Y++TI +      + FT T
Sbjct: 4   SDVVKASYWFPATD--FPATDIDSSLFTHLFCTFADLEAESYEITIATWNQAPFHAFTET 63

Query: 73  VTLSNPDVKTLLSIQGSN---DVFAQIAKDPTNRDVFIKSSIEAARAGSFDGLDLQWLYP 132
           V   NP VKTLLSI G N   D FA +A +P +R  FI+S+I  AR+  F GLDL W YP
Sbjct: 64  VQQRNPHVKTLLSIGGGNADKDAFASMASNPDSRASFIQSTITVARSYGFHGLDLDWEYP 123

Query: 133 SSTEEMKNFETFLVLWRKAVVEDAKNLSVPELLLVASVSNLPYLTHNIQ---YPIQTIKQ 192
            + EEM +F   L  WR AV  ++ +     L+L A+V    Y + N Q   YP+  I  
Sbjct: 124 RNEEEMYDFGKLLEEWRSAVEAESNSSGTTALILTAAV----YYSSNYQGVPYPVLAISN 183

Query: 193 KLNWVNVISYDFYTPTSSKDATGPSSAFNNPKANALSANFGIKSWIQALGNLPRNQIVFG 252
            L+W+N+++YDFY P  S   TGP ++   P  +  S + G++ W +A   LP  + V G
Sbjct: 184 SLDWINLMAYDFYGPGWS-TVTGPPASLYLP-TDGRSGDSGVRDWTEA--GLPAKKAVLG 243

Query: 253 IPFHGWAWKLSNSHQHDVFSKADGAAQGDYILEGGEIYYSNVKKFIDDNQAEVVNNDKRF 312
            P++GWAW L++    DV +  D    G  I + GEI Y  ++ +I DN A  V++D   
Sbjct: 244 FPYYGWAWTLADP---DV-NGYDANTTGPAISDDGEISYRQLQTWIVDNGATKVHDD-MM 303

Query: 313 LIAYTYFDTTWIAFESEYTIADKISLAKSIKVLGYFASNIAADDD 352
           +  Y Y  TTWI ++SE +I  K+  AK   +LGYF+ ++  DD+
Sbjct: 304 VGDYCYAGTTWIGYDSEKSIVTKVIYAKQKGLLGYFSWHVGGDDN 333

BLAST of Lsi11G003740 vs. TAIR 10
Match: AT4G19750.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 221.9 bits (564), Expect = 8.6e-58
Identity = 133/339 (39.23%), Postives = 191/339 (56.34%), Query Frame = 0

Query: 15  VVKAGYWLATTDPILPSANIDAMLFTHLFVGYASVDSTTYKVTIDSSQLVLINQFTSTVT 74
           +VKA YW+   +   P+ NID+  FTHLF  +A VDS+T++VTI ++    ++ FT TV 
Sbjct: 13  IVKASYWVVKPENDFPAGNIDSTRFTHLFCAFADVDSSTHEVTISAANSCQVSSFTHTVK 72

Query: 75  LSNPDVKTLLSIQGSN---DVFAQIAKDPTNRDVFIKSSIEAARAGSFDGLDLQWLYPSS 134
             N DV+TLLSI G +    V A +A +  NR  FI SSI+ AR   F GLDL W YPS+
Sbjct: 73  DKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLDLAWEYPSN 132

Query: 135 TEEMKNFETFLVLWRKAVVEDAKNLSVPELLLVASVSNLPYLTHNIQYPIQTIKQKLNWV 194
             EM NF   +  WR AVVE++   +   LLL A+V   P   +  +YP+Q I   L++V
Sbjct: 133 DVEMANFGKLVKEWRAAVVEESDRTNQLPLLLTAAVYYSPDY-YGEEYPVQAIADNLDFV 192

Query: 195 NVISYDFYTPTSSKDATGPSSAFNNPKANA-LSANFGIKSWIQALGNLPRNQIVFGIPFH 254
           N+++YDFY P  S   TGP +A  +P   A  S + G+  W++A   LP  + V G  + 
Sbjct: 193 NIMAYDFYGPGWS-PVTGPPAALFDPSNPAGRSGDSGLSKWLEA--KLPAKKAVLGFSYC 252

Query: 255 GWAWKLSNSHQHDVFSKADGAAQGDYILEGGEIYYSNVKKFIDDNQAEVVNNDKRFLIAY 314
           GWAW L ++  +   +  DGAA    I   G I Y+ ++ +I DN A    +D   +  Y
Sbjct: 253 GWAWTLEDAENNGYDAATDGAA----ISSDGSITYAKIRNYIIDNGAATF-HDPAVIGFY 312

Query: 315 TYFDTTWIAFESEYTIADKISLAKSIKVLGYFASNIAAD 350
            Y  TTWI ++   +I  K+  AK   +LGYF+ ++ AD
Sbjct: 313 CYVGTTWIGYDDNQSIVSKVRYAKLKGLLGYFSWHVGAD 342

BLAST of Lsi11G003740 vs. TAIR 10
Match: AT4G19760.1 (Glycosyl hydrolase family protein with chitinase insertion domain )

HSP 1 Score: 216.5 bits (550), Expect = 3.6e-56
Identity = 136/361 (37.67%), Postives = 194/361 (53.74%), Query Frame = 0

Query: 15  VVKAGYWL--------ATTDPILPSANIDAMLFTHLFVGYASVDSTTYKVTIDSSQLVLI 74
           +VKA YW                PS+ ID+ LFTHLF  +A VDS+T++VTI ++     
Sbjct: 13  IVKASYWFPDGKSQSPECLSQGTPSSFIDSTLFTHLFCAFADVDSSTHEVTISAANSYQF 72

Query: 75  NQFTSTVTLSNPDVKTLLSIQGSN---DVFAQIAKDPTNRDVFIKSSIEAARAGSFDGLD 134
           + FT TV   N DV+TLLSI G +    V A +A +  NR  FI SSI+ AR   F GLD
Sbjct: 73  SSFTETVKEKNTDVQTLLSIGGKDADKAVLASMASNSKNRKAFIDSSIDIARKKDFYGLD 132

Query: 135 LQWLYPSSTEEMKNFETFLVLWRKAVVEDAKNLSVPELLLVASVSNLPYLTHNIQYPIQT 194
           L W YPS+  EM NF   L  WR AVVE++   +   LLL A+V   P     ++YP++ 
Sbjct: 133 LAWEYPSNDVEMTNFGKLLEEWRAAVVEESDKTNQLPLLLTAAVYYSPQY-DGVEYPVKA 192

Query: 195 IKQKLNWVNVISYDFYTPTSSKDATGPSSAFNNPKANA-LSANFGIKSWIQALGNLPRNQ 254
           I   L++VN+++YDFY P  S     P++ F++P   A  S N G++ W+     LP  +
Sbjct: 193 IADNLDFVNIMAYDFYGPGWSPVTGPPAALFHDPSNPAGRSGNSGLRKWLDE-AKLPPKK 252

Query: 255 IVFGIPFHGWAWKLSNSHQHDVFSKADGAAQGDYILEGGEIYYSNVKKFIDDNQAEVVNN 314
            V G P+ GWAW L ++  +   +  DGAA    I   G I Y+ ++ +I DN A    +
Sbjct: 253 AVLGFPYCGWAWTLEDAENNGYDAATDGAA----ISPDGSITYAKIRNYIVDNGAATF-H 312

Query: 315 DKRFLIAYTYFDTTWIAFESEYTIADKISLAKSIKVLGYFASNIAADDDVNTLANAALAN 364
           D   +  Y Y   TWI ++   +I  K+  AK   +LGYF+ ++ AD +   L+ AA   
Sbjct: 313 DPAVIGFYCYVGNTWIGYDDNQSIVYKVKYAKFTGLLGYFSWHVGADYNCG-LSRAASIA 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O818629.2e-6541.81Class V chitinase OS=Arabidopsis thaliana OX=3702 GN=ChiC PE=1 SV=1[more]
A0A072UR652.1e-4835.44Class V chitinase CHIT5b OS=Medicago truncatula OX=3880 GN=CHIT5B PE=1 SV=1[more]
U5N4E33.9e-4734.15Nod factor hydrolase protein 1 OS=Medicago truncatula OX=3880 GN=NFH1 PE=1 SV=2[more]
A0A1B1J8Z21.6e-3730.75Class V chitinase CHIT5 OS=Lotus japonicus OX=34305 GN=CHIT5 PE=1 SV=2[more]
A0A072VEP01.8e-3631.03Class V chitinase CHIT5a OS=Medicago truncatula OX=3880 GN=CHIT5A PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KB823.1e-14069.89chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493904 PE=4 SV=1[more]
A0A6J1G2P44.1e-14070.17acidic mammalian chitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111450275 PE... [more]
A0A6J1KFT35.3e-14069.60chitotriosidase-1-like OS=Cucurbita maxima OX=3661 GN=LOC111493907 PE=4 SV=1[more]
A0A6J1G4H51.2e-13669.60chitotriosidase-1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450666 ... [more]
A0A6J1G4J91.2e-13669.60acidic mammalian chitinase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
Match NameE-valueIdentityDescription
XP_023521369.14.5e-14171.02acidic mammalian chitinase-like [Cucurbita pepo subsp. pepo][more]
XP_023545402.13.8e-14071.55acidic mammalian chitinase-like, partial [Cucurbita pepo subsp. pepo][more]
XP_022999577.16.4e-14069.89chitotriosidase-1-like [Cucurbita maxima][more]
XP_022946077.18.4e-14070.17acidic mammalian chitinase-like [Cucurbita moschata] >XP_022946671.1 acidic mamm... [more]
XP_022999580.11.1e-13969.60chitotriosidase-1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G19810.16.6e-6641.81Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19820.12.6e-6239.71Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19800.11.0e-5839.42Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19750.18.6e-5839.23Glycosyl hydrolase family protein with chitinase insertion domain [more]
AT4G19760.13.6e-5637.67Glycosyl hydrolase family protein with chitinase insertion domain [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011583Chitinase IISMARTSM006362g34coord: 15..349
e-value: 4.2E-55
score: 199.0
IPR029070Chitinase insertion domain superfamilyGENE3D3.10.50.10coord: 249..320
e-value: 1.0E-72
score: 247.3
IPR029070Chitinase insertion domain superfamilySUPERFAMILY54556Chitinase insertion domaincoord: 251..320
IPR001223Glycoside hydrolase family 18, catalytic domainPFAMPF00704Glyco_hydro_18coord: 19..348
e-value: 7.9E-50
score: 170.4
IPR001223Glycoside hydrolase family 18, catalytic domainPROSITEPS51910GH18_2coord: 15..363
score: 40.390579
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 33..350
e-value: 1.0E-72
score: 247.3
NoneNo IPR availablePANTHERPTHR11177:SF351CHITINASE-LIKE PROTEIN-RELATEDcoord: 11..357
NoneNo IPR availablePANTHERPTHR11177CHITINASEcoord: 11..357
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 19..353

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi11G003740.1Lsi11G003740.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0008061 chitin binding
molecular_function GO:0016787 hydrolase activity