Lsi11G003440 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi11G003440
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	calmodulin-like protein 7
Location	chr11: 3584257 .. 3584952 (-)
RNA-Seq Expression	Lsi11G003440
Synteny	Lsi11G003440

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACCCATCATGAATTTTCTGTTATTGGCAGTTTTATTCGTTGCTGGTTTTGTCAATTTCCTTATTTATTTCCCCACCAAGAGATTCACCGCTTGGTTCCAATCTATAAAACCCTCTCCTCAAATTTCCCATTTCAAATCCACTCCCCTCCAACCACCGCCACCACCGTCGCCACCGTCACCGGAGCTGAAGAAAGTGTTTGGGACATTCGACAAGAACAACGATGGGTTCATAACGAAGAAGGAGCTGATGGAATCGCTAAAGAACATGAGGATGATGATTACAGAGAAAGATGCAGAGGAAATGTTGAAAGGGGTTGATGAAAATGGAGATGGGTTGATTGATTTTGAGGAGTTTTGTGTTTTGGGAGAGAAATTAGTGATGGGTTTTGAAGAAAAAAAGAAAAGAAAGATTGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGTGAAGAAGATGAAGATGAATTGAAAGAGGCTTTTGGTGTGTTTGATAAAGATAAAGATGGATTGATTTGTGTGGAGGAATTGAGTTTGGTATTATGTTCTTTAGGGATGAATGAAGGGAAGAAATTTGAGAATTGTAAGGAAATGATAAGGAAAGTGGATTTGGATGGAGATGGAATGGTGAATTTTGATGAGTTTAAGAGGATGATGAGAAGTGGAGTAAGTCTTGTTTTAAATCTTAAATCTTAA

mRNA sequence

ATGGAACCCATCATGAATTTTCTGTTATTGGCAGTTTTATTCGTTGCTGGTTTTGTCAATTTCCTTATTTATTTCCCCACCAAGAGATTCACCGCTTGGTTCCAATCTATAAAACCCTCTCCTCAAATTTCCCATTTCAAATCCACTCCCCTCCAACCACCGCCACCACCGTCGCCACCGTCACCGGAGCTGAAGAAAGTGTTTGGGACATTCGACAAGAACAACGATGGGTTCATAACGAAGAAGGAGCTGATGGAATCGCTAAAGAACATGAGGATGATGATTACAGAGAAAGATGCAGAGGAAATGTTGAAAGGGGTTGATGAAAATGGAGATGGGTTGATTGATTTTGAGGAGTTTTGTGTTTTGGGAGAGAAATTAGTGATGGGTTTTGAAGAAAAAAAGAAAAGAAAGATTGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGTGAAGAAGATGAAGATGAATTGAAAGAGGCTTTTGGTGTGTTTGATAAAGATAAAGATGGATTGATTTGTGTGGAGGAATTGAGTTTGGTATTATGTTCTTTAGGGATGAATGAAGGGAAGAAATTTGAGAATTGTAAGGAAATGATAAGGAAAGTGGATTTGGATGGAGATGGAATGGTGAATTTTGATGAGTTTAAGAGGATGATGAGAAGTGGAGTAAGTCTTGTTTTAAATCTTAAATCTTAA

Coding sequence (CDS)

ATGGAACCCATCATGAATTTTCTGTTATTGGCAGTTTTATTCGTTGCTGGTTTTGTCAATTTCCTTATTTATTTCCCCACCAAGAGATTCACCGCTTGGTTCCAATCTATAAAACCCTCTCCTCAAATTTCCCATTTCAAATCCACTCCCCTCCAACCACCGCCACCACCGTCGCCACCGTCACCGGAGCTGAAGAAAGTGTTTGGGACATTCGACAAGAACAACGATGGGTTCATAACGAAGAAGGAGCTGATGGAATCGCTAAAGAACATGAGGATGATGATTACAGAGAAAGATGCAGAGGAAATGTTGAAAGGGGTTGATGAAAATGGAGATGGGTTGATTGATTTTGAGGAGTTTTGTGTTTTGGGAGAGAAATTAGTGATGGGTTTTGAAGAAAAAAAGAAAAGAAAGATTGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGGTGAAGAAGATGAAGATGAATTGAAAGAGGCTTTTGGTGTGTTTGATAAAGATAAAGATGGATTGATTTGTGTGGAGGAATTGAGTTTGGTATTATGTTCTTTAGGGATGAATGAAGGGAAGAAATTTGAGAATTGTAAGGAAATGATAAGGAAAGTGGATTTGGATGGAGATGGAATGGTGAATTTTGATGAGTTTAAGAGGATGATGAGAAGTGGAGTAAGTCTTGTTTTAAATCTTAAATCTTAA

Protein sequence

MEPIMNFLLLAVLFVAGFVNFLIYFPTKRFTAWFQSIKPSPQISHFKSTPLQPPPPPSPPSPELKKVFGTFDKNNDGFITKKELMESLKNMRMMITEKDAEEMLKGVDENGDGLIDFEEFCVLGEKLVMGFEEKKKRKIEEEEEEEEEEGEEDEDELKEAFGVFDKDKDGLICVEELSLVLCSLGMNEGKKFENCKEMIRKVDLDGDGMVNFDEFKRMMRSGVSLVLNLKS

Homology

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BLAST of Lsi11G003440 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 6.6e-32
Identity = 76/160 (47.50%), Postives = 107/160 (66.88%), Query Frame = 0


Query: 63  ELKKVFGTFDKNNDGFITKKELMESLKNMRMMITEKDAEEMLKGVDENGDGLIDFEEFCV 122
           EL ++F  FD+N DG ITK+EL +SL+N+ + I +KD  +M++ +D NGDG +D EEF  
Sbjct: 5   ELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGG 64

Query: 123 LGEKLVMGFEEKKKRKIEEEEEEEEEEGEEDEDELKEAFGVFDKDKDGLICVEELSLVLC 182
           L + ++              EE +EEE      +++EAF VFD+++DG I VEEL  VL 
Sbjct: 65  LYQTIM--------------EERDEEE------DMREAFNVFDQNRDGFITVEELRSVLA 124

Query: 183 SLGMNEGKKFENCKEMIRKVDLDGDGMVNFDEFKRMMRSG 223
           SLG+ +G+  E+CK MI KVD+DGDGMVNF EFK+MM+ G
Sbjct: 125 SLGLKQGRTLEDCKRMISKVDVDGDGMVNFKEFKQMMKGG 144

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BLAST of Lsi11G003440 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 9.5e-31
Identity = 75/160 (46.88%), Postives = 108/160 (67.50%), Query Frame = 0


Query: 63  ELKKVFGTFDKNNDGFITKKELMESLKNMRMMITEKDAEEMLKGVDENGDGLIDFEEFCV 122
           ELK+VF  FDKN DG IT KEL E+L+++ + I +K+  +M++ +D NGDG +D +E   
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDE--- 64

Query: 123 LGEKLVMGFEEKKKRKIEEEEEEEEEEGEEDEDELKEAFGVFDKDKDGLICVEELSLVLC 182
                   F E  K  ++EE+EEEE        ++KEAF VFD++ DG I V+EL  VL 
Sbjct: 65  --------FGELYKTIMDEEDEEEE--------DMKEAFNVFDQNGDGFITVDELKAVLS 124

Query: 183 SLGMNEGKKFENCKEMIRKVDLDGDGMVNFDEFKRMMRSG 223
           SLG+ +GK  ++CK+MI+KVD+DGDG VN+ EF++MM+ G
Sbjct: 125 SLGLKQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMMKGG 145

[Close]

BLAST of Lsi11G003440 vs. ExPASy Swiss-Prot
Match: O22845 (Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2)

HSP 1 Score: 134.8 bits (338), Expect = 1.2e-30
Identity = 85/201 (42.29%), Postives = 119/201 (59.20%), Query Frame = 0


Query: 32  AWF-----QSIKPSPQISHFKSTPLQPPPPPSPPS-----PELKKVFGTFDKNNDGFITK 91
           +WF     ++  PSP      ST L  P   S P+      ELK+VF  FDKN DG ITK
Sbjct: 30  SWFDKTLHKNSPPSP------STMLPSPSSSSAPTKRIDPSELKRVFQMFDKNGDGRITK 89

Query: 92  KELMESLKNMRMMITEKDAEEMLKGVDENGDGLIDFEEFCVLGEKLVMGFEEKKKRKIEE 151
           +EL +SL+N+ + I +KD  +M+  +D NGDG +D +EF  L   +V             
Sbjct: 90  EELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDIDEFESLYSSIV------------- 149

Query: 152 EEEEEEEEGEEDEDELKEAFGVFDKDKDGLICVEELSLVLCSLGMNEGKKFENCKEMIRK 211
             +E   +GE +E+++K+AF VFD+D DG I VEEL  V+ SLG+ +GK  + CK+MI +
Sbjct: 150 --DEHHNDGETEEEDMKDAFNVFDQDGDGFITVEELKSVMASLGLKQGKTLDGCKKMIMQ 209

Query: 212 VDLDGDGMVNFDEFKRMMRSG 223
           VD DGDG VN+ EF +MM+ G
Sbjct: 210 VDADGDGRVNYKEFLQMMKGG 209

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BLAST of Lsi11G003440 vs. ExPASy Swiss-Prot
Match: Q9ZR02 (Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.6e-30
Identity = 75/162 (46.30%), Postives = 107/162 (66.05%), Query Frame = 0


Query: 61  SPELKKVFGTFDKNNDGFITKKELMESLKNMRMMITEKDAEEMLKGVDENGDGLIDFEEF 120
           S EL +VF  FDK+ DG IT KEL ES KN+ ++I E +  ++++ +D NGDG +D EEF
Sbjct: 3   STELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEF 62

Query: 121 CVLGEKLVMGFEEKKKRKIEEEEEEEEEEGEEDEDELKEAFGVFDKDKDGLICVEELSLV 180
             L + +++              E+E+E GEED   +KEAF VFD++ DG I V+EL  V
Sbjct: 63  GELYKTIMV--------------EDEDEVGEED---MKEAFNVFDRNGDGFITVDELKAV 122

Query: 181 LCSLGMNEGKKFENCKEMIRKVDLDGDGMVNFDEFKRMMRSG 223
           L SLG+ +GK  E C++MI +VD+DGDG VN+ EF++MM+ G
Sbjct: 123 LSSLGLKQGKTLEECRKMIMQVDVDGDGRVNYMEFRQMMKKG 147

[Close]

BLAST of Lsi11G003440 vs. ExPASy Swiss-Prot
Match: Q9SU00 (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 6.1e-30
Identity = 75/160 (46.88%), Postives = 103/160 (64.38%), Query Frame = 0


Query: 63  ELKKVFGTFDKNNDGFITKKELMESLKNMRMMITEKDAEEMLKGVDENGDGLIDFEEFCV 122
           EL +VF  FDKN DG I K EL +  K++ +M+ E +  EM+  +D NGDG +D +EF  
Sbjct: 5   ELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGS 64

Query: 123 LGEKLVMGFEEKKKRKIEEEEEEEEEEGEEDEDELKEAFGVFDKDKDGLICVEELSLVLC 182
           L +++V              EE+EEEE      +++EAF VFD++ DG I  EEL  VL 
Sbjct: 65  LYQEMV--------------EEKEEEE------DMREAFRVFDQNGDGFITDEELRSVLA 124

Query: 183 SLGMNEGKKFENCKEMIRKVDLDGDGMVNFDEFKRMMRSG 223
           S+G+ +G+  E+CK+MI KVD+DGDGMVNF EFK+MMR G
Sbjct: 125 SMGLKQGRTLEDCKKMISKVDVDGDGMVNFKEFKQMMRGG 144

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BLAST of Lsi11G003440 vs. ExPASy TrEMBL
Match: A0A0A0LL19 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G007990 PE=4 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 6.3e-94
Identity = 187/235 (79.57%), Postives = 205/235 (87.23%), Query Frame = 0


Query: 1   MEPIMNFLLLAVLFVAGFVNFLIYFPTKRFTAWFQSIKPSPQISHFKSTPLQPPPPPSP- 60
           MEPI NFLLL+VLFVAGF+NFL+YFP+KRF+AWFQSIKPS QI+HFKSTPLQPPPPPSP 
Sbjct: 1   MEPIFNFLLLSVLFVAGFINFLLYFPSKRFSAWFQSIKPSSQITHFKSTPLQPPPPPSPS 60

Query: 61  --PSP------ELKKVFGTFDKNNDGFITKKELMESLKNMRMMITEKDAEEMLKGVDENG 120
             PSP      E+KKVFGTFDKN+DGFITKKELMESLK+MRMMITEKDAEEMLKGVDENG
Sbjct: 61  PSPSPPPPSAMEMKKVFGTFDKNDDGFITKKELMESLKSMRMMITEKDAEEMLKGVDENG 120

Query: 121 DGLIDFEEFCVLGEKLVMGFEEKKKRKIEEEEEEEEEEGEEDEDELKEAFGVFDKDKDGL 180
           DGLIDFEEFCVLG KL+MGFEE KK  +E+E           EDELK+AFGVFDKD DGL
Sbjct: 121 DGLIDFEEFCVLGGKLMMGFEENKKTSVEDE-----------EDELKDAFGVFDKDSDGL 180

Query: 181 ICVEELSLVLCSLGMNEGKKFENCKEMIRKVDLDGDGMVNFDEFKRMMRSGVSLV 227
           I VEELSLVLCSLGMNEGK  ENCKEMIRKVDLDGDGMVNFDEFK+MMR+GV+++
Sbjct: 181 ISVEELSLVLCSLGMNEGKIVENCKEMIRKVDLDGDGMVNFDEFKKMMRNGVTIL 224

[Close]

BLAST of Lsi11G003440 vs. ExPASy TrEMBL
Match: A0A1S3C5X4 (calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103496824 PE=4 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 1.4e-93
Identity = 187/233 (80.26%), Postives = 200/233 (85.84%), Query Frame = 0


Query: 1   MEPIMNFLLLAVLFVAGFVNFLIYFPTKRFTAWFQSIKPSPQISHFKSTPLQPPPPPSPP 60
           MEPI NFLLL+VLFVAGFVNFL+YFPTKRFTAWFQSIKPS QI HFKSTPLQPPPPP PP
Sbjct: 1   MEPIFNFLLLSVLFVAGFVNFLLYFPTKRFTAWFQSIKPSSQIPHFKSTPLQPPPPPPPP 60

Query: 61  SP-------ELKKVFGTFDKNNDGFITKKELMESLKNMRMMITEKDAEEMLKGVDENGDG 120
            P       ELKKVFGTFDKN+DGFITKKELMESLK+MRMMITEKDAEEMLK VDENGDG
Sbjct: 61  PPPPPPSAMELKKVFGTFDKNDDGFITKKELMESLKSMRMMITEKDAEEMLKEVDENGDG 120

Query: 121 LIDFEEFCVLGEKLVMGFEEKKKRKIEEEEEEEEEEGEEDEDELKEAFGVFDKDKDGLIC 180
           LIDFEEFCVLGEKL+MGFEE KK  +            +DE+ LK+AFGVFDKD DGLI 
Sbjct: 121 LIDFEEFCVLGEKLLMGFEENKKTSV-----------GDDEEGLKDAFGVFDKDSDGLIS 180

Query: 181 VEELSLVLCSLGMNEGKKFENCKEMIRKVDLDGDGMVNFDEFKRMMRSGVSLV 227
           VEELSLVLCSLGMNEGK  ENCKEMIRKVDLDGDGMVNFDEFK+MMR+GVS++
Sbjct: 181 VEELSLVLCSLGMNEGKIVENCKEMIRKVDLDGDGMVNFDEFKKMMRNGVSIL 222

[Close]

BLAST of Lsi11G003440 vs. ExPASy TrEMBL
Match: A0A6J1KJL5 (calmodulin-like protein 7 OS=Cucurbita maxima OX=3661 GN=LOC111493780 PE=4 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 1.3e-88
Identity = 180/229 (78.60%), Postives = 195/229 (85.15%), Query Frame = 0


Query: 1   MEPIMNFLLLAVLFVAGFVNFLIYFPTKRFTAWFQSIKPSPQISHFKSTPLQPP-PPPSP 60
           ME +MN L LAVLFVAGFVNFL+YFPTKRFTAWFQSIKPSPQ  HFK T + PP P P  
Sbjct: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPPSPAPEK 60

Query: 61  PSPELKKVFGTFDKNNDGFITKKELMESLKNMRMMITEKDAEEMLKGVDENGDGLIDFEE 120
              E+KKVFGTFDKNNDGFITKKELMESLKNM MMITE++AEEM+KGVDENGDGLIDFEE
Sbjct: 61  KKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120

Query: 121 FCVLGEKLVMGFEEKKKRKIEEEEEEEEEEGEEDEDELKEAFGVFDKDKDGLICVEELSL 180
           FCVLGEKLV+GFEE K+R+IE          +EDEDEL+EAFGVFDKDKDG+I VEEL L
Sbjct: 121 FCVLGEKLVVGFEE-KQRRIE----------DEDEDELREAFGVFDKDKDGVISVEELGL 180

Query: 181 VLCSLGMNEGKKFENCKEMIRKVDLDGDGMVNFDEFKRMMRSGVSLVLN 229
           VL  LGMNEGKK ENCKEMI+KVDLDGDGMVNFDEFKRMMRSG +LV N
Sbjct: 181 VLSCLGMNEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 218

[Close]

BLAST of Lsi11G003440 vs. ExPASy TrEMBL
Match: A0A6J1G4Z1 (calmodulin-like protein 7 OS=Cucurbita moschata OX=3662 GN=LOC111450782 PE=4 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 2.3e-88
Identity = 180/229 (78.60%), Postives = 197/229 (86.03%), Query Frame = 0


Query: 1   MEPIMNFLLLAVLFVAGFVNFLIYFPTKRFTAWFQSIKPSPQISHFKSTPLQP-PPPPSP 60
           ME +MN L LAVLFVAGFVNFL+YFPTKRFTAWFQSIKPSPQ  HFK T + P  P P  
Sbjct: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60

Query: 61  PSPELKKVFGTFDKNNDGFITKKELMESLKNMRMMITEKDAEEMLKGVDENGDGLIDFEE 120
              E+KKVFGTFDKN DGFITKKELMESLKNM MMITE++AEEM+KGVDENGDGLIDFEE
Sbjct: 61  KKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120

Query: 121 FCVLGEKLVMGFEEKKKRKIEEEEEEEEEEGEEDEDELKEAFGVFDKDKDGLICVEELSL 180
           FCVLGEKLVMGFEE K+R++E+E+E      +EDEDEL+EAFGVFDKDKDG+I VEEL L
Sbjct: 121 FCVLGEKLVMGFEE-KQRRVEDEDE------DEDEDELREAFGVFDKDKDGVISVEELGL 180

Query: 181 VLCSLGMNEGKKFENCKEMIRKVDLDGDGMVNFDEFKRMMRSGVSLVLN 229
           VL  LGMNEGKK ENCKEMI+KVDLDGDGMVNFDEFKRMMRSG +LV N
Sbjct: 181 VLSCLGMNEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 222

[Close]

BLAST of Lsi11G003440 vs. ExPASy TrEMBL
Match: A0A5A7VDD4 (Calmodulin-like protein 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00680 PE=4 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 1.5e-63
Identity = 132/164 (80.49%), Postives = 143/164 (87.20%), Query Frame = 0


Query: 63  ELKKVFGTFDKNNDGFITKKELMESLKNMRMMITEKDAEEMLKGVDENGDGLIDFEEFCV 122
           ELKKVFGTFDKN+DGFITKKELMESLK+MRMMITEKDAEEMLK VDENGDGLIDFEEFCV
Sbjct: 2   ELKKVFGTFDKNDDGFITKKELMESLKSMRMMITEKDAEEMLKEVDENGDGLIDFEEFCV 61

Query: 123 LGEKLVMGFEEKKKRKIEEEEEEEEEEGEEDEDELKEAFGVFDKDKDGLICVEELSLVLC 182
           LGEKL+MGFEE KK  +            +DE+ LK+AFGVFDKD DGLI VEELSLVLC
Sbjct: 62  LGEKLLMGFEENKKTSV-----------GDDEEGLKDAFGVFDKDSDGLISVEELSLVLC 121

Query: 183 SLGMNEGKKFENCKEMIRKVDLDGDGMVNFDEFKRMMRSGVSLV 227
           SLGMNEGK  ENCKEMIRKVDLDGDGMVNFDEFK+MMR+GVS++
Sbjct: 122 SLGMNEGKIVENCKEMIRKVDLDGDGMVNFDEFKKMMRNGVSIL 154

[Close]

BLAST of Lsi11G003440 vs. NCBI nr
Match: XP_038889113.1 (calmodulin-like protein 3 [Benincasa hispida])

HSP 1 Score: 378.3 bits (970), Expect = 4.9e-101
Identity = 201/229 (87.77%), Postives = 209/229 (91.27%), Query Frame = 0


Query: 1   MEPIMNFLLLAVLFVAGFVNFLIYFPTKRFTAWFQSIKPSPQISHFKSTPLQ--PPPPPS 60
           MEPIMNFLLL VL VAGFVNFL+YFPTKRF+AWFQSIKPSPQISHFKS  LQ  PPPPPS
Sbjct: 1   MEPIMNFLLLVVLVVAGFVNFLLYFPTKRFSAWFQSIKPSPQISHFKSLSLQPPPPPPPS 60

Query: 61  PPSPELKKVFGTFDKNNDGFITKKELMESLKNMRMMITEKDAEEMLKGVDENGDGLIDFE 120
           PPSPELKKVFGTFDKNNDGFITKKELMESLKNMRM+ITEKDAEEMLKGVDENGDGLIDFE
Sbjct: 61  PPSPELKKVFGTFDKNNDGFITKKELMESLKNMRMLITEKDAEEMLKGVDENGDGLIDFE 120

Query: 121 EFCVLGEKLVMGFEEKKKRKIEEEEEEEEEEGEEDEDELKEAFGVFDKDKDGLICVEELS 180
           EFCVLGEKLVMGF EKKK  +E+         EEDEDEL+EAFGVFDKDKDG+I VEELS
Sbjct: 121 EFCVLGEKLVMGFGEKKKICVED---------EEDEDELREAFGVFDKDKDGMISVEELS 180

Query: 181 LVLCSLGMNEGKKFENCKEMIRKVDLDGDGMVNFDEFKRMMRSGVSLVL 228
           LVLCSLGMNEGKK ENCKEMI+KVDLDGDGMVNFDEFKRMMRSGVSLVL
Sbjct: 181 LVLCSLGMNEGKKVENCKEMIKKVDLDGDGMVNFDEFKRMMRSGVSLVL 220

[Close]

BLAST of Lsi11G003440 vs. NCBI nr
Match: XP_004139293.1 (calmodulin-like protein 3 [Cucumis sativus] >KGN60706.1 hypothetical protein Csa_019409 [Cucumis sativus])

HSP 1 Score: 353.6 bits (906), Expect = 1.3e-93
Identity = 187/235 (79.57%), Postives = 205/235 (87.23%), Query Frame = 0


Query: 1   MEPIMNFLLLAVLFVAGFVNFLIYFPTKRFTAWFQSIKPSPQISHFKSTPLQPPPPPSP- 60
           MEPI NFLLL+VLFVAGF+NFL+YFP+KRF+AWFQSIKPS QI+HFKSTPLQPPPPPSP 
Sbjct: 1   MEPIFNFLLLSVLFVAGFINFLLYFPSKRFSAWFQSIKPSSQITHFKSTPLQPPPPPSPS 60

Query: 61  --PSP------ELKKVFGTFDKNNDGFITKKELMESLKNMRMMITEKDAEEMLKGVDENG 120
             PSP      E+KKVFGTFDKN+DGFITKKELMESLK+MRMMITEKDAEEMLKGVDENG
Sbjct: 61  PSPSPPPPSAMEMKKVFGTFDKNDDGFITKKELMESLKSMRMMITEKDAEEMLKGVDENG 120

Query: 121 DGLIDFEEFCVLGEKLVMGFEEKKKRKIEEEEEEEEEEGEEDEDELKEAFGVFDKDKDGL 180
           DGLIDFEEFCVLG KL+MGFEE KK  +E+E           EDELK+AFGVFDKD DGL
Sbjct: 121 DGLIDFEEFCVLGGKLMMGFEENKKTSVEDE-----------EDELKDAFGVFDKDSDGL 180

Query: 181 ICVEELSLVLCSLGMNEGKKFENCKEMIRKVDLDGDGMVNFDEFKRMMRSGVSLV 227
           I VEELSLVLCSLGMNEGK  ENCKEMIRKVDLDGDGMVNFDEFK+MMR+GV+++
Sbjct: 181 ISVEELSLVLCSLGMNEGKIVENCKEMIRKVDLDGDGMVNFDEFKKMMRNGVTIL 224

[Close]

BLAST of Lsi11G003440 vs. NCBI nr
Match: XP_008457058.1 (PREDICTED: calmodulin-like protein 7 [Cucumis melo])

HSP 1 Score: 352.4 bits (903), Expect = 2.9e-93
Identity = 187/233 (80.26%), Postives = 200/233 (85.84%), Query Frame = 0


Query: 1   MEPIMNFLLLAVLFVAGFVNFLIYFPTKRFTAWFQSIKPSPQISHFKSTPLQPPPPPSPP 60
           MEPI NFLLL+VLFVAGFVNFL+YFPTKRFTAWFQSIKPS QI HFKSTPLQPPPPP PP
Sbjct: 1   MEPIFNFLLLSVLFVAGFVNFLLYFPTKRFTAWFQSIKPSSQIPHFKSTPLQPPPPPPPP 60

Query: 61  SP-------ELKKVFGTFDKNNDGFITKKELMESLKNMRMMITEKDAEEMLKGVDENGDG 120
            P       ELKKVFGTFDKN+DGFITKKELMESLK+MRMMITEKDAEEMLK VDENGDG
Sbjct: 61  PPPPPPSAMELKKVFGTFDKNDDGFITKKELMESLKSMRMMITEKDAEEMLKEVDENGDG 120

Query: 121 LIDFEEFCVLGEKLVMGFEEKKKRKIEEEEEEEEEEGEEDEDELKEAFGVFDKDKDGLIC 180
           LIDFEEFCVLGEKL+MGFEE KK  +            +DE+ LK+AFGVFDKD DGLI 
Sbjct: 121 LIDFEEFCVLGEKLLMGFEENKKTSV-----------GDDEEGLKDAFGVFDKDSDGLIS 180

Query: 181 VEELSLVLCSLGMNEGKKFENCKEMIRKVDLDGDGMVNFDEFKRMMRSGVSLV 227
           VEELSLVLCSLGMNEGK  ENCKEMIRKVDLDGDGMVNFDEFK+MMR+GVS++
Sbjct: 181 VEELSLVLCSLGMNEGKIVENCKEMIRKVDLDGDGMVNFDEFKKMMRNGVSIL 222

[Close]

BLAST of Lsi11G003440 vs. NCBI nr
Match: XP_022999398.1 (calmodulin-like protein 7 [Cucurbita maxima])

HSP 1 Score: 335.9 bits (860), Expect = 2.8e-88
Identity = 180/229 (78.60%), Postives = 195/229 (85.15%), Query Frame = 0


Query: 1   MEPIMNFLLLAVLFVAGFVNFLIYFPTKRFTAWFQSIKPSPQISHFKSTPLQPP-PPPSP 60
           ME +MN L LAVLFVAGFVNFL+YFPTKRFTAWFQSIKPSPQ  HFK T + PP P P  
Sbjct: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPPSPAPEK 60

Query: 61  PSPELKKVFGTFDKNNDGFITKKELMESLKNMRMMITEKDAEEMLKGVDENGDGLIDFEE 120
              E+KKVFGTFDKNNDGFITKKELMESLKNM MMITE++AEEM+KGVDENGDGLIDFEE
Sbjct: 61  KKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120

Query: 121 FCVLGEKLVMGFEEKKKRKIEEEEEEEEEEGEEDEDELKEAFGVFDKDKDGLICVEELSL 180
           FCVLGEKLV+GFEE K+R+IE          +EDEDEL+EAFGVFDKDKDG+I VEEL L
Sbjct: 121 FCVLGEKLVVGFEE-KQRRIE----------DEDEDELREAFGVFDKDKDGVISVEELGL 180

Query: 181 VLCSLGMNEGKKFENCKEMIRKVDLDGDGMVNFDEFKRMMRSGVSLVLN 229
           VL  LGMNEGKK ENCKEMI+KVDLDGDGMVNFDEFKRMMRSG +LV N
Sbjct: 181 VLSCLGMNEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 218

[Close]

BLAST of Lsi11G003440 vs. NCBI nr
Match: KAG6599380.1 (Calmodulin-like protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 335.5 bits (859), Expect = 3.6e-88
Identity = 180/229 (78.60%), Postives = 197/229 (86.03%), Query Frame = 0


Query: 1   MEPIMNFLLLAVLFVAGFVNFLIYFPTKRFTAWFQSIKPSPQISHFKSTPLQP-PPPPSP 60
           ME +MN L LAVLFVAGFVNFL+YFPTKRFTAWFQSIKPSPQ  HFK T + P  P P  
Sbjct: 1   MESVMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPTAVPPLSPAPEK 60

Query: 61  PSPELKKVFGTFDKNNDGFITKKELMESLKNMRMMITEKDAEEMLKGVDENGDGLIDFEE 120
              E+KKVFGTFDKN DGFITKKELMESLKNM MMITE++AEEM+KGVDENGDGLIDFEE
Sbjct: 61  KKSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 120

Query: 121 FCVLGEKLVMGFEEKKKRKIEEEEEEEEEEGEEDEDELKEAFGVFDKDKDGLICVEELSL 180
           FCVLGEKLVMGFEE K+R++E+E+E      +EDEDEL+EAFGVFDKDKDG+I VEEL L
Sbjct: 121 FCVLGEKLVMGFEE-KQRRVEDEDE------DEDEDELREAFGVFDKDKDGMISVEELGL 180

Query: 181 VLCSLGMNEGKKFENCKEMIRKVDLDGDGMVNFDEFKRMMRSGVSLVLN 229
           VL  LGMNEGKK ENCKEMI+KVDLDGDGMVNFDEFKRMMRSG +LV N
Sbjct: 181 VLSCLGMNEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVFN 222

[Close]

BLAST of Lsi11G003440 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 139.0 bits (349), Expect = 4.7e-33
Identity = 76/160 (47.50%), Postives = 107/160 (66.88%), Query Frame = 0


Query: 63  ELKKVFGTFDKNNDGFITKKELMESLKNMRMMITEKDAEEMLKGVDENGDGLIDFEEFCV 122
           EL ++F  FD+N DG ITK+EL +SL+N+ + I +KD  +M++ +D NGDG +D EEF  
Sbjct: 5   ELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGG 64

Query: 123 LGEKLVMGFEEKKKRKIEEEEEEEEEEGEEDEDELKEAFGVFDKDKDGLICVEELSLVLC 182
           L + ++              EE +EEE      +++EAF VFD+++DG I VEEL  VL 
Sbjct: 65  LYQTIM--------------EERDEEE------DMREAFNVFDQNRDGFITVEELRSVLA 124

Query: 183 SLGMNEGKKFENCKEMIRKVDLDGDGMVNFDEFKRMMRSG 223
           SLG+ +G+  E+CK MI KVD+DGDGMVNF EFK+MM+ G
Sbjct: 125 SLGLKQGRTLEDCKRMISKVDVDGDGMVNFKEFKQMMKGG 144

[Close]

BLAST of Lsi11G003440 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 135.2 bits (339), Expect = 6.7e-32
Identity = 75/160 (46.88%), Postives = 108/160 (67.50%), Query Frame = 0


Query: 63  ELKKVFGTFDKNNDGFITKKELMESLKNMRMMITEKDAEEMLKGVDENGDGLIDFEEFCV 122
           ELK+VF  FDKN DG IT KEL E+L+++ + I +K+  +M++ +D NGDG +D +E   
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDE--- 64

Query: 123 LGEKLVMGFEEKKKRKIEEEEEEEEEEGEEDEDELKEAFGVFDKDKDGLICVEELSLVLC 182
                   F E  K  ++EE+EEEE        ++KEAF VFD++ DG I V+EL  VL 
Sbjct: 65  --------FGELYKTIMDEEDEEEE--------DMKEAFNVFDQNGDGFITVDELKAVLS 124

Query: 183 SLGMNEGKKFENCKEMIRKVDLDGDGMVNFDEFKRMMRSG 223
           SLG+ +GK  ++CK+MI+KVD+DGDG VN+ EF++MM+ G
Sbjct: 125 SLGLKQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMMKGG 145

[Close]

BLAST of Lsi11G003440 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 134.8 bits (338), Expect = 8.8e-32
Identity = 85/201 (42.29%), Postives = 119/201 (59.20%), Query Frame = 0


Query: 32  AWF-----QSIKPSPQISHFKSTPLQPPPPPSPPS-----PELKKVFGTFDKNNDGFITK 91
           +WF     ++  PSP      ST L  P   S P+      ELK+VF  FDKN DG ITK
Sbjct: 30  SWFDKTLHKNSPPSP------STMLPSPSSSSAPTKRIDPSELKRVFQMFDKNGDGRITK 89

Query: 92  KELMESLKNMRMMITEKDAEEMLKGVDENGDGLIDFEEFCVLGEKLVMGFEEKKKRKIEE 151
           +EL +SL+N+ + I +KD  +M+  +D NGDG +D +EF  L   +V             
Sbjct: 90  EELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDIDEFESLYSSIV------------- 149

Query: 152 EEEEEEEEGEEDEDELKEAFGVFDKDKDGLICVEELSLVLCSLGMNEGKKFENCKEMIRK 211
             +E   +GE +E+++K+AF VFD+D DG I VEEL  V+ SLG+ +GK  + CK+MI +
Sbjct: 150 --DEHHNDGETEEEDMKDAFNVFDQDGDGFITVEELKSVMASLGLKQGKTLDGCKKMIMQ 209

Query: 212 VDLDGDGMVNFDEFKRMMRSG 223
           VD DGDG VN+ EF +MM+ G
Sbjct: 210 VDADGDGRVNYKEFLQMMKGG 209

[Close]

BLAST of Lsi11G003440 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 134.4 bits (337), Expect = 1.1e-31
Identity = 75/162 (46.30%), Postives = 107/162 (66.05%), Query Frame = 0


Query: 61  SPELKKVFGTFDKNNDGFITKKELMESLKNMRMMITEKDAEEMLKGVDENGDGLIDFEEF 120
           S EL +VF  FDK+ DG IT KEL ES KN+ ++I E +  ++++ +D NGDG +D EEF
Sbjct: 3   STELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEF 62

Query: 121 CVLGEKLVMGFEEKKKRKIEEEEEEEEEEGEEDEDELKEAFGVFDKDKDGLICVEELSLV 180
             L + +++              E+E+E GEED   +KEAF VFD++ DG I V+EL  V
Sbjct: 63  GELYKTIMV--------------EDEDEVGEED---MKEAFNVFDRNGDGFITVDELKAV 122

Query: 181 LCSLGMNEGKKFENCKEMIRKVDLDGDGMVNFDEFKRMMRSG 223
           L SLG+ +GK  E C++MI +VD+DGDG VN+ EF++MM+ G
Sbjct: 123 LSSLGLKQGKTLEECRKMIMQVDVDGDGRVNYMEFRQMMKKG 147

[Close]

BLAST of Lsi11G003440 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 132.5 bits (332), Expect = 4.4e-31
Identity = 75/160 (46.88%), Postives = 103/160 (64.38%), Query Frame = 0


Query: 63  ELKKVFGTFDKNNDGFITKKELMESLKNMRMMITEKDAEEMLKGVDENGDGLIDFEEFCV 122
           EL +VF  FDKN DG I K EL +  K++ +M+ E +  EM+  +D NGDG +D +EF  
Sbjct: 5   ELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGS 64

Query: 123 LGEKLVMGFEEKKKRKIEEEEEEEEEEGEEDEDELKEAFGVFDKDKDGLICVEELSLVLC 182
           L +++V              EE+EEEE      +++EAF VFD++ DG I  EEL  VL 
Sbjct: 65  LYQEMV--------------EEKEEEE------DMREAFRVFDQNGDGFITDEELRSVLA 124

Query: 183 SLGMNEGKKFENCKEMIRKVDLDGDGMVNFDEFKRMMRSG 223
           S+G+ +G+  E+CK+MI KVD+DGDGMVNF EFK+MMR G
Sbjct: 125 SMGLKQGRTLEDCKKMISKVDVDGDGMVNFKEFKQMMRGG 144

The following BLAST results are available for this feature:

BLAST of Lsi11G003440 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SRR7	6.6e-32	47.50	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]
Q9LNE7	9.5e-31	46.88	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]
O22845	1.2e-30	42.29	Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2	[more]
Q9ZR02	1.6e-30	46.30	Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1	[more]
Q9SU00	6.1e-30	46.88	Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1	[more]

BLAST of Lsi11G003440 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LL19	6.3e-94	79.57	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G007990 PE=4 SV=1	[more]
A0A1S3C5X4	1.4e-93	80.26	calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103496824 PE=4 SV=1	[more]
A0A6J1KJL5	1.3e-88	78.60	calmodulin-like protein 7 OS=Cucurbita maxima OX=3661 GN=LOC111493780 PE=4 SV=1	[more]
A0A6J1G4Z1	2.3e-88	78.60	calmodulin-like protein 7 OS=Cucurbita moschata OX=3662 GN=LOC111450782 PE=4 SV=...	[more]
A0A5A7VDD4	1.5e-63	80.49	Calmodulin-like protein 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]

BLAST of Lsi11G003440 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038889113.1	4.9e-101	87.77	calmodulin-like protein 3 [Benincasa hispida]	[more]
XP_004139293.1	1.3e-93	79.57	calmodulin-like protein 3 [Cucumis sativus] >KGN60706.1 hypothetical protein Csa...	[more]
XP_008457058.1	2.9e-93	80.26	PREDICTED: calmodulin-like protein 7 [Cucumis melo]	[more]
XP_022999398.1	2.8e-88	78.60	calmodulin-like protein 7 [Cucurbita maxima]	[more]
KAG6599380.1	3.6e-88	78.60	Calmodulin-like protein 4, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lsi11G003440 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07490.1	4.7e-33	47.50	ARF-GAP domain 11	[more]
AT1G05990.1	6.7e-32	46.88	EF hand calcium-binding protein family	[more]
AT2G43290.1	8.8e-32	42.29	Calcium-binding EF-hand family protein	[more]
AT4G03290.1	1.1e-31	46.30	EF hand calcium-binding protein family	[more]
AT4G12860.1	4.4e-31	46.88	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 128..160
None	No IPR available	GENE3D	1.10.238.10		coord: 128..228 e-value: 3.8E-21 score: 77.1
None	No IPR available	GENE3D	1.10.238.10		coord: 59..124 e-value: 1.7E-19 score: 71.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 48..63
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 135..157
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 44..63
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 142..157
None	No IPR available	PANTHER	PTHR10891:SF796	CALCIUM-BINDING PROTEIN CML30-RELATED	coord: 5..226
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 5..226
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 99..127 e-value: 0.0011 score: 28.2 coord: 156..184 e-value: 0.0019 score: 27.5 coord: 194..222 e-value: 3.3E-6 score: 36.6 coord: 63..91 e-value: 4.5E-5 score: 32.8
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 155..220 e-value: 1.9E-11 score: 44.2 coord: 63..121 e-value: 4.0E-12 score: 46.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 95..130 score: 12.253878
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 59..94 score: 12.588623
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 190..225 score: 13.788124
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 152..187 score: 14.290241
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 63..121 e-value: 1.00785E-15 score: 67.1877
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 160..220 e-value: 3.59496E-14 score: 62.9505
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 108..120
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 72..84
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 165..177
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 203..215
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 56..220

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi11G003440.1	Lsi11G003440.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005509	calcium ion binding