Lsi11G003020 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi11G003020
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionProtein kinase
Locationchr11: 3127972 .. 3130486 (-)
RNA-Seq ExpressionLsi11G003020
SyntenyLsi11G003020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATATTGTAGTTAAAAAAAGCCCACACTCAAAGTTGTCTATATATATATAAAGAAAAAAACCCACAAACCCAACATCAAACCCTCTCTTCTTTCTCCCTTTTTTTTTGTTAACTTTTTTTGTGAGACAGTAAAAAATAATTGGGGCGGGCGATGATGGCCGGAAGCATAGCCAGAGACGAGTGGTCCGATTACGACAGCAGTTGTTCCTCCTCCATAACCGTCCCGGACTCCACCCGCAGCTGGATGAGTAACCTCAGTTTCGGAAGCCGGCGCCGAAGCAGTAGCTCAGCCGCAGACGGCGGCGTTGAGATGATGACGACAACAACAACAACAGCAACATTACCAGTAGTAAAACTGACGATGAAGCCACACAAGGCGAATGAAGTGGGATGGGAGGCGATGCGGTGGCTCCGGGGAGTGAAAGGGGAAGTGGGGTTAGAGCATTTCCGGCTGCTACGGCGGGTGGGGAGTGGAGATATTGGGAATGTTTATTTATGTGAGATAAGGAATCCAATGGTGGGATTGCCAAAGTGCTTTTATGCAATGAAAGTGGTTGATAGAGAAGCGGTTTGTGTAAGGAATAAAGAGAGAAGGGCAGATATGGAAAAGGAAATATTGAAAGTGCTTGATCATCCTTTTTTGCCTACTCTTTATGCTCATTTTGAAGCTTCTCATTACTCTTGTTTGCTTATGGAGTTTTGCCCTGGTGGTGATTTGTATGCTGCTCGCCAACGTCAACCCAATAAACGTTTCTCCATTTCTTCTGCTAAGTAAGTCCTTTCTTTCTTTTTTATAAAAAACGAGTGCATGTCCATTATTACTAACGAATTATATCTACGCTTAATAATTTGACAATTACTTTTTTATAGTTATAAAAAATATTTTATCGGTTAAACTATGCTTGATTTATATATATATTTCTATTTCTAGATATCTCATTCTAATTTCTCACAAAAAAGAAAAAAATAGAAAAAAGTAAGGCGAGTTTATTGCCGGACAAATTGTGATGGAATAATTAGGTGAAATGAACAATGATGTGCTTTAATATATTTACGTATTTTCTTTTAATTTTAATAAATTGTTATTTTGATTAGTACAAAGAACTTATTAATTAGATGGGGACTAATTATGTGACACAGAAATTATTTTAGTAATTAAAATCGATATTTCTACATATTATCAACTAACATATTTTTATTGAGATCGGAAATTGAAATATGATCTTTATGAAATTCTTCATGTTGTATTGAAAATTAAATGTAAATCTACTTCATTTAGTTAAAATATATACTTTTAACTAAGAGATCTCTAAATTTTTTCATTGTGATATTATGTTGAACTAAGAAAGTAAGATGTTTTTTTTTTTTGAAAAGTTGAAAATACAATTTTCTTTGGAGAATTTTTAAAAATAGAAAAATAAGAAAACCATTTACATAAAATAACAAAAATTTTAAATAGTTGTGATAGATGCTGAATAAAAATTTATTCGTATTTTTTTTTACAATTTTCTATAAATAATTTGACATTTTTTTATATCCGTGAAAATTTCCCATTTTCTTATAATTTATGAGTTTTAATGGATTTTTTTTTGGACAGGTTTTATGCGGCGGAGATAATTTTAGCTCTAGAGTATTTGCACATGATGGGCATAATTTACAGAGATTTAAAGCCGGAAAACGTTTTGGTTAGAGAAGACGGCCACATAATGCTGTCGGATTTCGATCTCTGCCTTAAATGGGACGTCGTTCCAAAGCTCATCCGTTGCCGAACCACCGCCTCCGACGCCACCACCGAACGCCACGTCAGATCCTACTCCACCTCAACCACCTCCTGCGCTGCCGCCCCCATTCAACCCGTCCTCTCCTGTTTCTCCATTGCCCGTCGCAAAAAATCCACCACCGTAACTACCATCACCGAACACGCCGCCGCACCCTCCGCCGTGTACGGCGGCGACAGCGACGGAGAGGGCGAGGAATTCGACCCAGAACCAGAATTAATAGCGGAACCAATAAACGCTCGGTCGAAATCATTCGTCGGAACCCACGAGTATTTGGCGCCGGAGGTGATTTCCGGGCAAGGGCATGGCAGCGGCGTGGATTGGTGGACATTGGGAATATTCTTATACGAAATGTTGTACGGAAAAACACCATTTAAAGGGGAAAACAACGAGAAGACATTAATGAACATTTTGAAGCAGCCATTAAGATTTCCAAGAATTGGGTTTAGTAGCAGCAAAGAGTATGAAGAAATGGTGAAAGTTGAGGATTTAATCAAGAAGCTTTTGGTGAAAAATCCAAAAAAAAGAATTGGAAGTTTAAAGGGTTCAGTTGAAATCAAAAGACATGATTTTTTCAAAGGACTCAATTGGGCTTTAATTCGTTCCGTACGACCTCCACAAATCCCCAACAATAACCTTAATTACTCCAAATACCCTTCTAATTACTTACCCAAATTGAGCAAGAAACAAAGACAACAACCTTACCAAATCCCTAATCACCATTTTGATTATTTCTAA

mRNA sequence

ATATTGTAGTTAAAAAAAGCCCACACTCAAAGTTGTCTATATATATATAAAGAAAAAAACCCACAAACCCAACATCAAACCCTCTCTTCTTTCTCCCTTTTTTTTTGTTAACTTTTTTTGTGAGACAGTAAAAAATAATTGGGGCGGGCGATGATGGCCGGAAGCATAGCCAGAGACGAGTGGTCCGATTACGACAGCAGTTGTTCCTCCTCCATAACCGTCCCGGACTCCACCCGCAGCTGGATGAGTAACCTCAGTTTCGGAAGCCGGCGCCGAAGCAGTAGCTCAGCCGCAGACGGCGGCGTTGAGATGATGACGACAACAACAACAACAGCAACATTACCAGTAGTAAAACTGACGATGAAGCCACACAAGGCGAATGAAGTGGGATGGGAGGCGATGCGGTGGCTCCGGGGAGTGAAAGGGGAAGTGGGGTTAGAGCATTTCCGGCTGCTACGGCGGGTGGGGAGTGGAGATATTGGGAATGTTTATTTATGTGAGATAAGGAATCCAATGGTGGGATTGCCAAAGTGCTTTTATGCAATGAAAGTGGTTGATAGAGAAGCGGTTTGTGTAAGGAATAAAGAGAGAAGGGCAGATATGGAAAAGGAAATATTGAAAGTGCTTGATCATCCTTTTTTGCCTACTCTTTATGCTCATTTTGAAGCTTCTCATTACTCTTGTTTGCTTATGGAGTTTTGCCCTGGTGGTGATTTGTATGCTGCTCGCCAACGTCAACCCAATAAACGTTTCTCCATTTCTTCTGCTAAAGATTTAAAGCCGGAAAACGTTTTGGTTAGAGAAGACGGCCACATAATGCTGTCGGATTTCGATCTCTGCCTTAAATGGGACGTCGTTCCAAAGCTCATCCGTTGCCGAACCACCGCCTCCGACGCCACCACCGAACGCCACGTCAGATCCTACTCCACCTCAACCACCTCCTGCGCTGCCGCCCCCATTCAACCCGTCCTCTCCTGTTTCTCCATTGCCCGTCGCAAAAAATCCACCACCGTAACTACCATCACCGAACACGCCGCCGCACCCTCCGCCGTGTACGGCGGCGACAGCGACGGAGAGGGCGAGGAATTCGACCCAGAACCAGAATTAATAGCGGAACCAATAAACGCTCGGTCGAAATCATTCGTCGGAACCCACGAGTATTTGGCGCCGGAGGTGATTTCCGGGCAAGGGCATGGCAGCGGCGTGGATTGGTGGACATTGGGAATATTCTTATACGAAATGTTGTACGGAAAAACACCATTTAAAGGGGAAAACAACGAGAAGACATTAATGAACATTTTGAAGCAGCCATTAAGATTTCCAAGAATTGGGTTTAGTAGCAGCAAAGAGTATGAAGAAATGGTGAAAGTTGAGGATTTAATCAAGAAGCTTTTGGTGAAAAATCCAAAAAAAAGAATTGGAAGTTTAAAGGGTTCAGTTGAAATCAAAAGACATGATTTTTTCAAAGGACTCAATTGGGCTTTAATTCGTTCCGTACGACCTCCACAAATCCCCAACAATAACCTTAATTACTCCAAATACCCTTCTAATTACTTACCCAAATTGAGCAAGAAACAAAGACAACAACCTTACCAAATCCCTAATCACCATTTTGATTATTTCTAA

Coding sequence (CDS)

ATGATGGCCGGAAGCATAGCCAGAGACGAGTGGTCCGATTACGACAGCAGTTGTTCCTCCTCCATAACCGTCCCGGACTCCACCCGCAGCTGGATGAGTAACCTCAGTTTCGGAAGCCGGCGCCGAAGCAGTAGCTCAGCCGCAGACGGCGGCGTTGAGATGATGACGACAACAACAACAACAGCAACATTACCAGTAGTAAAACTGACGATGAAGCCACACAAGGCGAATGAAGTGGGATGGGAGGCGATGCGGTGGCTCCGGGGAGTGAAAGGGGAAGTGGGGTTAGAGCATTTCCGGCTGCTACGGCGGGTGGGGAGTGGAGATATTGGGAATGTTTATTTATGTGAGATAAGGAATCCAATGGTGGGATTGCCAAAGTGCTTTTATGCAATGAAAGTGGTTGATAGAGAAGCGGTTTGTGTAAGGAATAAAGAGAGAAGGGCAGATATGGAAAAGGAAATATTGAAAGTGCTTGATCATCCTTTTTTGCCTACTCTTTATGCTCATTTTGAAGCTTCTCATTACTCTTGTTTGCTTATGGAGTTTTGCCCTGGTGGTGATTTGTATGCTGCTCGCCAACGTCAACCCAATAAACGTTTCTCCATTTCTTCTGCTAAAGATTTAAAGCCGGAAAACGTTTTGGTTAGAGAAGACGGCCACATAATGCTGTCGGATTTCGATCTCTGCCTTAAATGGGACGTCGTTCCAAAGCTCATCCGTTGCCGAACCACCGCCTCCGACGCCACCACCGAACGCCACGTCAGATCCTACTCCACCTCAACCACCTCCTGCGCTGCCGCCCCCATTCAACCCGTCCTCTCCTGTTTCTCCATTGCCCGTCGCAAAAAATCCACCACCGTAACTACCATCACCGAACACGCCGCCGCACCCTCCGCCGTGTACGGCGGCGACAGCGACGGAGAGGGCGAGGAATTCGACCCAGAACCAGAATTAATAGCGGAACCAATAAACGCTCGGTCGAAATCATTCGTCGGAACCCACGAGTATTTGGCGCCGGAGGTGATTTCCGGGCAAGGGCATGGCAGCGGCGTGGATTGGTGGACATTGGGAATATTCTTATACGAAATGTTGTACGGAAAAACACCATTTAAAGGGGAAAACAACGAGAAGACATTAATGAACATTTTGAAGCAGCCATTAAGATTTCCAAGAATTGGGTTTAGTAGCAGCAAAGAGTATGAAGAAATGGTGAAAGTTGAGGATTTAATCAAGAAGCTTTTGGTGAAAAATCCAAAAAAAAGAATTGGAAGTTTAAAGGGTTCAGTTGAAATCAAAAGACATGATTTTTTCAAAGGACTCAATTGGGCTTTAATTCGTTCCGTACGACCTCCACAAATCCCCAACAATAACCTTAATTACTCCAAATACCCTTCTAATTACTTACCCAAATTGAGCAAGAAACAAAGACAACAACCTTACCAAATCCCTAATCACCATTTTGATTATTTCTAA

Protein sequence

MMAGSIARDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAADGGVEMMTTTTTTATLPVVKLTMKPHKANEVGWEAMRWLRGVKGEVGLEHFRLLRRVGSGDIGNVYLCEIRNPMVGLPKCFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLLMEFCPGGDLYAARQRQPNKRFSISSAKDLKPENVLVREDGHIMLSDFDLCLKWDVVPKLIRCRTTASDATTERHVRSYSTSTTSCAAAPIQPVLSCFSIARRKKSTTVTTITEHAAAPSAVYGGDSDGEGEEFDPEPELIAEPINARSKSFVGTHEYLAPEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLRFPRIGFSSSKEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRHDFFKGLNWALIRSVRPPQIPNNNLNYSKYPSNYLPKLSKKQRQQPYQIPNHHFDYF
Homology
BLAST of Lsi11G003020 vs. ExPASy Swiss-Prot
Match: Q94E49 (Protein kinase PINOID 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PID2 PE=2 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 3.4e-139
Identity = 278/516 (53.88%), Postives = 340/516 (65.89%), Query Frame = 0

Query: 10  EWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAADGGVEMMTTTTTTATLPVVKL 69
           E SDYDSS  SS+T PDS RSW+S++   S   S S+ + GG             P    
Sbjct: 6   EESDYDSS-RSSLTAPDSRRSWISDIGSSS---SVSARSFGG-----------DTPASSC 65

Query: 70  TMKPHKANEVGWEAMRWLRGVKGEVGLEHFRLLRRVGSGDIGNVYLCEIRNPMVGLPK-- 129
             KPHKAN+  WEA+R LR   G VGLEHFRL+RR+GSGD+GNVYLC +R P        
Sbjct: 66  RYKPHKANQAEWEAIRRLRAGAGRVGLEHFRLVRRLGSGDLGNVYLCRLREPWSSSSMTT 125

Query: 130 ----CFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLLMEF 189
               C YAMKVVD++A+  R K RRA++E++IL+ LDHPFLPTLYA FEASHY+CL+MEF
Sbjct: 126 TAGGCLYAMKVVDKDALAFRKKLRRAEVERDILRTLDHPFLPTLYADFEASHYACLVMEF 185

Query: 190 CPGGDLYAARQRQPNKRFSISSA---------------------KDLKPENVLVREDGHI 249
           CPGGDL+ ARQRQP +RF++SS                      +DLKPENVLVR DGHI
Sbjct: 186 CPGGDLHVARQRQPGRRFTVSSTRFYVAETVLALEYLHMMGVVYRDLKPENVLVRGDGHI 245

Query: 250 MLSDFDLCLKWDVVPKLIRCRTTASDATTERHVRSYSTSTTSCAAAPIQPVLSCFSIARR 309
           MLSDFDL LK DVVPKL+R   +A+        +      +SC    IQPVLSC      
Sbjct: 246 MLSDFDLSLKCDVVPKLLRPARSAAAGG-----KPPLPPPSSCVPPTIQPVLSCIFRGVH 305

Query: 310 KKSTTVTTITEHAAAPSAVYGGDSDGEGEEFDPEPE-LIAEPINARSKSFVGTHEYLAPE 369
           K           AAA +   G  +D E E    EPE ++ EP+ ARSKSFVGTHEYLAPE
Sbjct: 306 KCHHAKECAGGGAAAGNNGDGDGNDEEAETETAEPEVVVVEPVAARSKSFVGTHEYLAPE 365

Query: 370 VISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLRFPRI-GFSSSKE 429
           VISGQGHGS VDWWTLG+F+YEMLYG+TPFKGE+NEKTL+NI+KQP+ FPR+ G +++ E
Sbjct: 366 VISGQGHGSAVDWWTLGVFMYEMLYGRTPFKGESNEKTLINIIKQPVTFPRLAGAAAAGE 425

Query: 430 YEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRHDFFKGLNWALIRSVRPPQIPNNNLN 489
           +EEM   +DL+ +LL KNPKKR+GS  GS E+KRH FFKG+NWAL+RSVRPP++P     
Sbjct: 426 WEEMKTAQDLMLQLLAKNPKKRLGSTMGSAEVKRHPFFKGVNWALVRSVRPPEVP----- 485

Query: 490 YSKYPSNYLPK----LSKKQRQQPYQI-PNHHFDYF 492
               P    PK    +SKK+RQ+PY   P +HFDYF
Sbjct: 486 ---APPAPAPKKVMTMSKKERQEPYNYRPENHFDYF 493

BLAST of Lsi11G003020 vs. ExPASy Swiss-Prot
Match: Q64FQ2 (Protein kinase PINOID 2 OS=Arabidopsis thaliana OX=3702 GN=PID2 PE=1 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 4.2e-137
Identity = 285/536 (53.17%), Postives = 349/536 (65.11%), Query Frame = 0

Query: 11  WSDYDSSCSSSITVPDSTR--SWMSNLSFGSRRRSS-SSAADGGVEMMTTTTTTATLPVV 70
           + D +S   SS   PDS+R  SW+S+    S   SS S  ++ G+     +         
Sbjct: 8   YKDNESDYESSTVGPDSSRRTSWLSSSFTASPSCSSISHLSNHGLNSYNQS--------- 67

Query: 71  KLTMKPHKANEVGWEAMRWLRGVKGE-VGLEHFRLLRRVGSGDIGNVYLCEIRNPMVGLP 130
               KPHKAN+V WEAM  LR   G  VGLEHFRLL+R+GSGDIG+VYLC+IR    G P
Sbjct: 68  ----KPHKANQVAWEAMARLRRCCGRAVGLEHFRLLKRLGSGDIGSVYLCQIR----GSP 127

Query: 131 K-CFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLLMEFCP 190
           +  FYAMKVVD+EAV V+ K  RA+MEK+IL +LDHPF PTLYA FEASHYS L+ME+CP
Sbjct: 128 ETAFYAMKVVDKEAVAVKKKLGRAEMEKKILGMLDHPFCPTLYAAFEASHYSFLVMEYCP 187

Query: 191 GGDLYAARQRQPNKRFSISSA---------------------KDLKPENVLVREDGHIML 250
           GGDLYA R RQP+KRF+ISS                      +DLKPENVL+REDGH+ML
Sbjct: 188 GGDLYAVRLRQPSKRFTISSTRFYAAETLVALEYLHMMGIVYRDLKPENVLIREDGHVML 247

Query: 251 SDFDLCLKWDVVPKLI--RCRTTASDATTERHVRSYSTSTTSCAAAPIQPVLSCF---SI 310
           SDFDL  K DVVP+ +    R        +        ST SC   P+ PV+SCF   S 
Sbjct: 248 SDFDLSFKCDVVPQFLSDNDRDRGHQEDDDDISIRRKCSTPSCTTTPLNPVISCFSPTSS 307

Query: 311 ARRKKSTTVTTITEHAAAPS-AVYGGD---------------SDGEGEEFDPEPELIAEP 370
            RRKK+   TTI E+AA  S +V   D               S+G  +     P + AEP
Sbjct: 308 RRRKKNVVTTTIHENAAGTSDSVKSNDVSRTFSRSPSSCSRVSNGLRDISGGCPSIFAEP 367

Query: 371 INARSKSFVGTHEYLAPEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNI 430
           INARSKSFVGTHEYLAPEVISGQGHGS VDWWT GIFLYEM++G+TPFKG+NNEKTL+NI
Sbjct: 368 INARSKSFVGTHEYLAPEVISGQGHGSAVDWWTYGIFLYEMIFGRTPFKGDNNEKTLVNI 427

Query: 431 LKQPLRFPRIGFSSSKEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRHDFFKGLNW 490
           LK PL FP++  +S KEYE+MV  +DLI KLLVKNPKKR+GSLKGS+EIKRH+FF+G+NW
Sbjct: 428 LKAPLTFPKVIVNSPKEYEDMVNAQDLIIKLLVKNPKKRLGSLKGSIEIKRHEFFEGVNW 487

Query: 491 ALIRSVRPPQIPNNNLNYSKYPSN-----YLP---KLSKKQRQQPYQIPNHHFDYF 492
           ALIRS++PP +P    ++     N     YLP    +S+K+R +PY + N +FDYF
Sbjct: 488 ALIRSIKPPWVPKEETSHKTKGDNRSVNYYLPPRFMMSRKERNEPYHVSN-YFDYF 525

BLAST of Lsi11G003020 vs. ExPASy Swiss-Prot
Match: Q9SUA3 (Serine/threonine-protein kinase D6PKL1 OS=Arabidopsis thaliana OX=3702 GN=D6PKL1 PE=1 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 2.6e-102
Identity = 204/443 (46.05%), Postives = 263/443 (59.37%), Query Frame = 0

Query: 72  KPHKANEVGWEAMRWLRGVKGEVGLEHFRLLRRVGSGDIGNVYLCEIRNPMVGLPKCFYA 131
           KPHKAN+V WEA++ +R   G +GL HFRLL+R+G GDIG V+L E+        +CF+A
Sbjct: 96  KPHKANDVRWEAIQAVRTKHGVLGLNHFRLLKRLGCGDIGTVHLAELHG-----TRCFFA 155

Query: 132 MKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLLMEFCPGGDLYA 191
           MKV+D+ A+  R K  RA  E+EIL+ LDHPFLPTLY+HFE   +SCL+MEFCPGGDL+ 
Sbjct: 156 MKVMDKGALASRKKLLRAQTEREILQCLDHPFLPTLYSHFETEKFSCLVMEFCPGGDLHT 215

Query: 192 ARQRQPNKRFSISSAK---------------------DLKPENVLVREDGHIMLSDFDLC 251
            RQRQP KRFS  +AK                     DLKPENVLVR+DGH+MLSDFDL 
Sbjct: 216 LRQRQPGKRFSEQAAKFYVAEVLLAMEYLHMLGIIYRDLKPENVLVRDDGHVMLSDFDLS 275

Query: 252 LKWDVVPKLIRCRTTASDATTERH--VRSYSTSTTSCAAAPIQPVLSCFSIARRKKSTTV 311
           L+  V P ++R    AS+         +       SC +AP       +  ++ KK   +
Sbjct: 276 LRCTVSPTVVRSTVLASEGQKNSGYCAQPACIQQPSCISAPTTCFSPRYFSSKSKKDKKM 335

Query: 312 TTITEHAAAPSAVYGGDSDGEGEEFDPEPELIAEPINARSKSFVGTHEYLAPEVISGQGH 371
              T                 G +  P PEL+AEP +ARS SFVGTHEYLAPE+I G+GH
Sbjct: 336 KNET-----------------GNQVSPLPELVAEPTSARSMSFVGTHEYLAPEIIKGEGH 395

Query: 372 GSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLRFPRIGFSSSKEYEEMVKVE 431
           GS VDWWT GIFLYE+L+GKTPFKG  N  TL N++ QPLRFP     S           
Sbjct: 396 GSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVVGQPLRFPESPVVS-------FAAR 455

Query: 432 DLIKKLLVKNPKKRIGSLKGSVEIKRHDFFKGLNWALIRSVRPPQIPNNNLNYSKYPSNY 491
           DLI+ LLVK P+ R+   +G+ E+K+H FF+G+NWAL+R   PP+IP   ++Y   P+  
Sbjct: 456 DLIRSLLVKEPQHRLAYKRGATEMKQHPFFEGVNWALVRCASPPEIP-KPVDYESAPAT- 506

BLAST of Lsi11G003020 vs. ExPASy Swiss-Prot
Match: Q9FG74 (Serine/threonine-protein kinase D6PK OS=Arabidopsis thaliana OX=3702 GN=D6PK PE=1 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 6.3e-101
Identity = 197/408 (48.28%), Postives = 253/408 (62.01%), Query Frame = 0

Query: 72  KPHKANEVGWEAMRWLRGVKGEVGLEHFRLLRRVGSGDIGNVYLCEIRNPMVGLPKCFYA 131
           KPHKAN+V WEA++ +R   G +GL HFRLL+R+G GDIG V+L E+        +C++A
Sbjct: 82  KPHKANDVRWEAIQAVRTKHGGLGLNHFRLLKRLGCGDIGTVHLAELNG-----TRCYFA 141

Query: 132 MKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLLMEFCPGGDLYA 191
           MKV+D+ A+  R K  RA  E+EIL+ LDHPFLPTLY+HFE   +SCL+MEFCPGGDL+ 
Sbjct: 142 MKVMDKTALASRKKLLRAQTEREILQCLDHPFLPTLYSHFETEKFSCLVMEFCPGGDLHT 201

Query: 192 ARQRQPNKRFSISSAK---------------------DLKPENVLVREDGHIMLSDFDLC 251
            RQRQP KRF+  +AK                     DLKPENVLVR+DGH+MLSDFDL 
Sbjct: 202 LRQRQPGKRFTEQAAKFYVAEVLLAMEYLHMLGIIYRDLKPENVLVRDDGHVMLSDFDLS 261

Query: 252 LKWDVVPKLIRCRTTASDATTERHVRSYSTSTTSCAAAPI---QPVLSCFSIARRKKSTT 311
           L+  V   ++R     S+          S ++ SC+  P    QP  SC S+A     T+
Sbjct: 262 LRCTVSLSIVRSANVGSE--------GLSKNSVSCSQQPACIQQP--SCISMA----PTS 321

Query: 312 VTTITEHAAAPSAVYGGDSDGEGEEFDPEPELIAEPINARSKSFVGTHEYLAPEVISGQG 371
                  ++         ++    +  P PEL+AEP  ARS SFVGTHEYLAPE+I G+G
Sbjct: 322 CFGPRFFSSKSKKDKKPKTENGNHQVTPLPELVAEPTGARSMSFVGTHEYLAPEIIKGEG 381

Query: 372 HGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLRFPRIGFSSSKEYEEMVKV 431
           HGS VDWWT GIFLYE+L+GKTPFKG  N  TL N++ QPLRFP     S          
Sbjct: 382 HGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVVGQPLRFPESPVVS-------FAA 441

Query: 432 EDLIKKLLVKNPKKRIGSLKGSVEIKRHDFFKGLNWALIRSVRPPQIP 456
            DLI+ LLVK P+ R+   +G+ EIK+H FF+G+NWAL+R   PP+IP
Sbjct: 442 RDLIRSLLVKEPQHRLAYKRGATEIKQHPFFEGVNWALVRCASPPEIP 463

BLAST of Lsi11G003020 vs. ExPASy Swiss-Prot
Match: A2YBX5 (Protein kinase G11A OS=Oryza sativa subsp. indica OX=39946 GN=OsI_021818 PE=2 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 2.3e-98
Identity = 207/453 (45.70%), Postives = 264/453 (58.28%), Query Frame = 0

Query: 29  RSWMSNLSFGSRRRSSSSAADGGVEMMTTTTTTATLPVVKLTMKPHKANEVGWEAMRWLR 88
           RS      F S R   S+++D   E   ++ ++ T        KPHKAN+  WEA++ +R
Sbjct: 133 RSETGERGFSSSRCRPSTSSDVSDESACSSISSVT--------KPHKANDSRWEAIQMIR 192

Query: 89  GVKGEVGLEHFRLLRRVGSGDIGNVYLCEIRNPMVGLPKCFYAMKVVDREAVCVRNKERR 148
              G +GL HF+LL+++G GDIG+VYL E+        K ++AMKV+D+ ++  R K  R
Sbjct: 193 TRDGILGLSHFKLLKKLGCGDIGSVYLSELNG-----TKSYFAMKVMDKASLASRKKLLR 252

Query: 149 ADMEKEILKVLDHPFLPTLYAHFEASHYSCLLMEFCPGGDLYAARQRQPNKRFSISSAK- 208
           A  EKEIL+ LDHPFLPTLY HFE   +SCL+MEFCPGGDL+  RQRQ  K F   + K 
Sbjct: 253 AQTEKEILQCLDHPFLPTLYTHFETDKFSCLVMEFCPGGDLHTLRQRQRGKYFPEQAVKF 312

Query: 209 --------------------DLKPENVLVREDGHIMLSDFDLCLKWDVVPKLIRCRTTAS 268
                               DLKPENVLVREDGHIMLSDFDL L+  V P LIR     +
Sbjct: 313 YVAEILLAMEYLHMLGIIYRDLKPENVLVREDGHIMLSDFDLSLRCAVSPTLIRSSNPDA 372

Query: 269 DATTERHVRSYSTSTT----SCAAAP-IQPVLSCFSIARRKKSTTVTTITEHAAAPSAVY 328
           +A  + + ++Y         SC   P      +CF      KS       +    P  V 
Sbjct: 373 EALRKNN-QAYCVQPACVEPSCMIQPSCATPTTCFGPRFFSKSK-----KDRKPKPEVV- 432

Query: 329 GGDSDGEGEEFDPEPELIAEPINARSKSFVGTHEYLAPEVISGQGHGSGVDWWTLGIFLY 388
                    +  P PELIAEP +ARS SFVGTHEYLAPE+I G+GHGS VDWWT GIFLY
Sbjct: 433 --------NQVSPWPELIAEPSDARSMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLY 492

Query: 389 EMLYGKTPFKGENNEKTLMNILKQPLRFPRIGFSSSKEYEEMVKVEDLIKKLLVKNPKKR 448
           E+L+GKTPFKG  N  TL N++ QPLRFP     S           DLI+ LLVK P++R
Sbjct: 493 ELLFGKTPFKGSGNRATLFNVIGQPLRFPEYPVVS-------FSARDLIRGLLVKEPQQR 550

Query: 449 IGSLKGSVEIKRHDFFKGLNWALIRSVRPPQIP 456
           +G  +G+ EIK+H FF+G+NWALIR   PP++P
Sbjct: 553 LGCKRGATEIKQHPFFEGVNWALIRCASPPEVP 550

BLAST of Lsi11G003020 vs. ExPASy TrEMBL
Match: A0A5D3BGM8 (Protein kinase PINOID 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00110 PE=4 SV=1)

HSP 1 Score: 838.6 bits (2165), Expect = 1.4e-239
Identity = 436/520 (83.85%), Postives = 457/520 (87.88%), Query Frame = 0

Query: 2   MAGSIARDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAAD-GGVEMMTTTTT 61
           MAGSI RDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAAD GG+E +T+ TT
Sbjct: 1   MAGSIGRDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAADGGGLENITSGTT 60

Query: 62  TATLPVVKLTMKPHKANEVGWEAMRWLRGVKGEVGLEHFRLLRRVGSGDIGNVYLCEIRN 121
           T T+ V     KPHKANEVGWEAMRWLR  KGEVGLE FRL+RRVGSGDIGNVYLCEIRN
Sbjct: 61  TTTMMV-----KPHKANEVGWEAMRWLRRAKGEVGLEDFRLIRRVGSGDIGNVYLCEIRN 120

Query: 122 PMVGLPKCFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLL 181
           PMVGLPK FYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCL+
Sbjct: 121 PMVGLPKWFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLV 180

Query: 182 MEFCPGGDLYAARQRQPNKRFSISSAK---------------------DLKPENVLVRED 241
           MEFCPGGDLYAAR R PNKRFSI SAK                     DLKPENVLVRED
Sbjct: 181 MEFCPGGDLYAARLRLPNKRFSIPSAKFYAAEIILALEYLHMMGIIYRDLKPENVLVRED 240

Query: 242 GHIMLSDFDLCLKWDVVPKLIRCRTTASDATTERHVRSYS---TSTTSCAAAPIQPVLSC 301
           GHIMLSDFDLCLK DVVPKL+R RTTASD    RH+RS S   ++++SCAAAPIQPVLSC
Sbjct: 241 GHIMLSDFDLCLKCDVVPKLLRSRTTASD----RHLRSSSYTFSTSSSCAAAPIQPVLSC 300

Query: 302 FSIARRKKSTTVTTITEHAAAPSAVYGGDSDGEGEEFDPEPELIAEPINARSKSFVGTHE 361
           FS++RR+KSTTVTTITEHA+   ++YG   D  GEEFDPEPELIAEPINARSKSFVGTHE
Sbjct: 301 FSLSRRQKSTTVTTITEHAS--DSIYGPGDD--GEEFDPEPELIAEPINARSKSFVGTHE 360

Query: 362 YLAPEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLRFPRIGFS 421
           YLAPEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPL+FPRIG S
Sbjct: 361 YLAPEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLKFPRIGIS 420

Query: 422 SSKEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRHDFFKGLNWALIRSVRPPQIP- 481
           SSKEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRH+FFKG+NWALIRSVRPPQIP 
Sbjct: 421 SSKEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRHEFFKGINWALIRSVRPPQIPN 480

Query: 482 ----NNNLNYSKYPSNYLPKLSKKQRQQPYQIPNHHFDYF 492
               NNNLNYSKYPSNYLPKLSKKQRQQPYQIPNHHFDYF
Sbjct: 481 NNIINNNLNYSKYPSNYLPKLSKKQRQQPYQIPNHHFDYF 507

BLAST of Lsi11G003020 vs. ExPASy TrEMBL
Match: A0A1S3C6E4 (protein kinase PINOID 2 OS=Cucumis melo OX=3656 GN=LOC103497373 PE=4 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 8.8e-239
Identity = 435/520 (83.65%), Postives = 457/520 (87.88%), Query Frame = 0

Query: 2   MAGSIARDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAAD-GGVEMMTTTTT 61
           MAGSI RDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAAD GG+E +T+ TT
Sbjct: 1   MAGSIGRDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAADGGGLENITSGTT 60

Query: 62  TATLPVVKLTMKPHKANEVGWEAMRWLRGVKGEVGLEHFRLLRRVGSGDIGNVYLCEIRN 121
           T T+ V     KPHKANEVGWEAMRWLR  KGEVGLE FRL+RRVGSGDIGNVYLCEIRN
Sbjct: 61  TTTMMV-----KPHKANEVGWEAMRWLRRAKGEVGLEDFRLIRRVGSGDIGNVYLCEIRN 120

Query: 122 PMVGLPKCFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLL 181
           PMVGLPK FYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCL+
Sbjct: 121 PMVGLPKWFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLV 180

Query: 182 MEFCPGGDLYAARQRQPNKRFSISSAK---------------------DLKPENVLVRED 241
           MEFCPGGDLYAAR R PNKRFSI SAK                     DLKPENVLVRED
Sbjct: 181 MEFCPGGDLYAARLRLPNKRFSIPSAKFYAAEIILALEYLHMMGIIYRDLKPENVLVRED 240

Query: 242 GHIMLSDFDLCLKWDVVPKLIRCRTTASDATTERHVRSYS---TSTTSCAAAPIQPVLSC 301
           GHIMLSDFDLCLK DVVPKL+R RTTASD    RH+RS S   ++++SCAAAPIQPVLSC
Sbjct: 241 GHIMLSDFDLCLKCDVVPKLLRSRTTASD----RHLRSSSYTFSTSSSCAAAPIQPVLSC 300

Query: 302 FSIARRKKSTTVTTITEHAAAPSAVYGGDSDGEGEEFDPEPELIAEPINARSKSFVGTHE 361
           FS++RR+KSTTVTTITE+A+   ++YG   D  GEEFDPEPELIAEPINARSKSFVGTHE
Sbjct: 301 FSLSRRQKSTTVTTITENAS--DSIYGPGDD--GEEFDPEPELIAEPINARSKSFVGTHE 360

Query: 362 YLAPEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLRFPRIGFS 421
           YLAPEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPL+FPRIG S
Sbjct: 361 YLAPEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLKFPRIGIS 420

Query: 422 SSKEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRHDFFKGLNWALIRSVRPPQIP- 481
           SSKEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRH+FFKG+NWALIRSVRPPQIP 
Sbjct: 421 SSKEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRHEFFKGINWALIRSVRPPQIPN 480

Query: 482 ----NNNLNYSKYPSNYLPKLSKKQRQQPYQIPNHHFDYF 492
               NNNLNYSKYPSNYLPKLSKKQRQQPYQIPNHHFDYF
Sbjct: 481 NNIINNNLNYSKYPSNYLPKLSKKQRQQPYQIPNHHFDYF 507

BLAST of Lsi11G003020 vs. ExPASy TrEMBL
Match: A0A6J1HPF1 (protein kinase PINOID 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465514 PE=4 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 1.7e-210
Identity = 393/519 (75.72%), Postives = 425/519 (81.89%), Query Frame = 0

Query: 2   MAGSIARDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAADGGVEMMTTTTTT 61
           MAG I RD+ SD+DS+C SS+T+PDS+RSWMSNLSFGS RRSS+SA D  +         
Sbjct: 1   MAG-ITRDD-SDHDSTC-SSLTLPDSSRSWMSNLSFGS-RRSSTSALDAALS-------- 60

Query: 62  ATLPVVKLTMKPHKANEVGWEAMRWLRGVKGEVGLEHFRLLRRVGSGDIGNVYLCEIRNP 121
                     KPHKAN+V WEAMRW+R VKG VGLEHFRLLRRVGSGDIGNVYLCEIRNP
Sbjct: 61  --------PYKPHKANQVAWEAMRWVRRVKGRVGLEHFRLLRRVGSGDIGNVYLCEIRNP 120

Query: 122 MVGLPKCFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLLM 181
           +VGL +C+YAMKVVDREAV VRNK RRADMEKEILK++DHPFLPTLYA FEASHYSCL+M
Sbjct: 121 VVGLAQCYYAMKVVDREAVSVRNKGRRADMEKEILKLIDHPFLPTLYAQFEASHYSCLVM 180

Query: 182 EFCPGGDLYAARQRQPNKRFSISSAK---------------------DLKPENVLVREDG 241
           EFCPGGDLYAARQR P KRF I S K                     DLKPENVLVREDG
Sbjct: 181 EFCPGGDLYAARQRLPGKRFPIPSVKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDG 240

Query: 242 HIMLSDFDLCLKWDVVPKLIRCRTTASDATTERHV-RSYST-STTSCAAAPIQPVLSCFS 301
           HIMLSDFDLCLK DVVPKLIRCRTTA     ERHV ++YST STTSCAAAPIQPVLSCFS
Sbjct: 241 HIMLSDFDLCLKCDVVPKLIRCRTTA-----ERHVTKTYSTSSTTSCAAAPIQPVLSCFS 300

Query: 302 IARRKKSTTVTTITEHAAAPSAVYGGDSDGEGEEFDPEPELIAEPINARSKSFVGTHEYL 361
           I RR+ STT+TTITEHAA  +A   G       EFDPEPELIAEP+NARSKSFVGTHEYL
Sbjct: 301 ITRRRNSTTITTITEHAAESAATVDG-------EFDPEPELIAEPMNARSKSFVGTHEYL 360

Query: 362 APEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLRFPRIGFSSS 421
           APEVISGQGHGS VDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPL FPRIG SS+
Sbjct: 361 APEVISGQGHGSAVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLTFPRIGVSSN 420

Query: 422 KEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRHDFFKGLNWALIRSVRPPQIPNNN 481
           KEYEEMVKVEDLI+KLLVKNPKKRIGSLKGSVEIKRH+FFKG+NWALIRSVRPPQ+PN++
Sbjct: 421 KEYEEMVKVEDLIRKLLVKNPKKRIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQVPNSD 480

Query: 482 ------LNYSKYPSNYLPKLSKKQRQQPYQIPNHHFDYF 492
                 L  SKYPSNY+PKL+KKQRQQPYQIP+ HFDYF
Sbjct: 481 HLLHKTLTQSKYPSNYVPKLTKKQRQQPYQIPSPHFDYF 487

BLAST of Lsi11G003020 vs. ExPASy TrEMBL
Match: A0A6J1FGS3 (protein kinase PINOID 2-like OS=Cucurbita moschata OX=3662 GN=LOC111443882 PE=4 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 2.1e-208
Identity = 389/516 (75.39%), Postives = 420/516 (81.40%), Query Frame = 0

Query: 5   SIARDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAADGGVEMMTTTTTTATL 64
           SI RD+ SD+DS+ SSS+T+ DSTRSW+SNLS  S RRSS+SA D  +            
Sbjct: 3   SITRDD-SDHDST-SSSLTLQDSTRSWLSNLSISS-RRSSTSALDAALS----------- 62

Query: 65  PVVKLTMKPHKANEVGWEAMRWLRGVKGEVGLEHFRLLRRVGSGDIGNVYLCEIRNPMVG 124
                  KPHKAN V WEAMRW+R VKG VGLEHFRLLRRVGSGDIGNVYLCEIRNP+VG
Sbjct: 63  -----PYKPHKANHVAWEAMRWVRRVKGRVGLEHFRLLRRVGSGDIGNVYLCEIRNPVVG 122

Query: 125 LPKCFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLLMEFC 184
           L +C+YAMKVVDREAV VRNK RRADMEKEILK++DHPFLPTLYA FEASHYSCL+MEFC
Sbjct: 123 LAQCYYAMKVVDREAVSVRNKGRRADMEKEILKLIDHPFLPTLYAEFEASHYSCLVMEFC 182

Query: 185 PGGDLYAARQRQPNKRFSISSAK---------------------DLKPENVLVREDGHIM 244
           PGGDLYAARQR P KRF ISS+K                     DLKPENVLVREDGHIM
Sbjct: 183 PGGDLYAARQRLPGKRFPISSSKFYAAEIILALEYLHMMGIVYRDLKPENVLVREDGHIM 242

Query: 245 LSDFDLCLKWDVVPKLIRCRTTASDATTERHV-RSYST-STTSCAAAPIQPVLSCFSIAR 304
           LSDFDLCLK DVVPKLIRCRTTA     ERHV ++YST STTSCAAAPI PVLSCFSI R
Sbjct: 243 LSDFDLCLKCDVVPKLIRCRTTA-----ERHVTKTYSTSSTTSCAAAPIHPVLSCFSITR 302

Query: 305 RKKSTTVTTITEHAAAPSAVYGGDSDGEGEEFDPEPELIAEPINARSKSFVGTHEYLAPE 364
           R  STT+TTITEHAA  +A   G       EFDPEPELIAEP+NARSKSFVGTHEYLAPE
Sbjct: 303 RPNSTTITTITEHAAESAATVDG-------EFDPEPELIAEPMNARSKSFVGTHEYLAPE 362

Query: 365 VISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLRFPRIGFSSSKEY 424
           VISGQGHGS VDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPL FPRIG SS+KEY
Sbjct: 363 VISGQGHGSAVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLTFPRIGVSSNKEY 422

Query: 425 EEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRHDFFKGLNWALIRSVRPPQIPNNN--- 484
           EEMVKVEDLI+KLLVKNPKKRIGSLKGSVEIKRH+FFKG+NWALIRSVRPPQ+PN++   
Sbjct: 423 EEMVKVEDLIRKLLVKNPKKRIGSLKGSVEIKRHEFFKGVNWALIRSVRPPQVPNSDHLL 482

Query: 485 ---LNYSKYPSNYLPKLSKKQRQQPYQIPNHHFDYF 492
              L  SKYPSNY+PKL+KKQRQQPYQIP+HHFDYF
Sbjct: 483 HKTLTQSKYPSNYVPKLTKKQRQQPYQIPSHHFDYF 487

BLAST of Lsi11G003020 vs. ExPASy TrEMBL
Match: A0A6J1KGU7 (protein kinase PINOID 2-like OS=Cucurbita maxima OX=3661 GN=LOC111493762 PE=4 SV=1)

HSP 1 Score: 733.8 bits (1893), Expect = 4.7e-208
Identity = 397/513 (77.39%), Postives = 423/513 (82.46%), Query Frame = 0

Query: 5   SIARDEWSDYDSSCSSSITVPDSTRSWMSNLSFGS-RRRSSSSAADGGVEMMTTTTTTAT 64
           +IA+DE SDYDSSC SSIT+PDSTRSWMSNLSFGS RRRSSSSA DG            T
Sbjct: 3   AIAKDE-SDYDSSC-SSITLPDSTRSWMSNLSFGSQRRRSSSSATDG------------T 62

Query: 65  LPVVKLTMKPHKANEVGWEAMRWLRGVKGEVGLEHFRLLRRVGSGDIGNVYLCEIR--NP 124
            PV     KPHKANEV WEAMRWLRG KGEVGLE+FRLLRR+GSGDIGNVYLC+IR   P
Sbjct: 63  SPV----QKPHKANEVAWEAMRWLRGAKGEVGLENFRLLRRLGSGDIGNVYLCQIRKPTP 122

Query: 125 MVGLPKCFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLLM 184
              + +C YAMKVVDREAVCVRNKERRA+MEKEILK++DHPFLPTLYA FEASHYSCL+M
Sbjct: 123 TARVGQCCYAMKVVDREAVCVRNKERRAEMEKEILKMIDHPFLPTLYAAFEASHYSCLVM 182

Query: 185 EFCPGGDLYAARQRQPNKRFSISSAK---------------------DLKPENVLVREDG 244
           EFCPGGDLYAAR RQP KRFSISSAK                     DLKPENVLVR DG
Sbjct: 183 EFCPGGDLYAARLRQPAKRFSISSAKFYAAEIILALEYLHMMGIVYRDLKPENVLVRHDG 242

Query: 245 HIMLSDFDLCLKWDVVPKLIRCRTTASDATTERHVRSYST-STTSCAAAPIQPVLSCFSI 304
           HIMLSDFDLCLK DVVPKLIR RTTASD  TER+VRSYST STTSCAA PI+PVLSCFSI
Sbjct: 243 HIMLSDFDLCLKCDVVPKLIRSRTTASD-VTERYVRSYSTSSTTSCAAVPIKPVLSCFSI 302

Query: 305 ARRKKSTTVTTITEHAAAPSAVYGGDSDGEGEEFDPEPELIAEPINARSKSFVGTHEYLA 364
           ARR+K TTVTTITEHA         D DG  EEFD EPEL+AEPINARSKSFVGTHEYLA
Sbjct: 303 ARRRKPTTVTTITEHA---------DDDGYREEFDTEPELVAEPINARSKSFVGTHEYLA 362

Query: 365 PEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLRFPRIGFSSSK 424
           PEVISGQGHGS VDWWT+GIFLYEMLYGKTPFKGENNEKTLMNIL+QPL FPRIGFSSSK
Sbjct: 363 PEVISGQGHGSAVDWWTVGIFLYEMLYGKTPFKGENNEKTLMNILRQPLTFPRIGFSSSK 422

Query: 425 EYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRHDFFKGLNWALIRSVRPPQIPN-NN 484
           EYEEMVKVEDLIKKLLVKNPKKRIGSLKGS+EIKRH+FFKG+NWALIRSVRPP IPN ++
Sbjct: 423 EYEEMVKVEDLIKKLLVKNPKKRIGSLKGSIEIKRHEFFKGVNWALIRSVRPPHIPNSDH 482

Query: 485 LNYSKYPSNYLPKLSKKQRQQPYQIPNHHFDYF 492
           LN    P +  PKLSK+QRQQPYQIP+  FDYF
Sbjct: 483 LN----PRSKNPKLSKQQRQQPYQIPSDRFDYF 483

BLAST of Lsi11G003020 vs. NCBI nr
Match: XP_038889440.1 (LOW QUALITY PROTEIN: protein kinase PINOID 2 [Benincasa hispida])

HSP 1 Score: 867.5 bits (2240), Expect = 5.7e-248
Identity = 446/516 (86.43%), Postives = 462/516 (89.53%), Query Frame = 0

Query: 4   GSIARDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAADGGVEMMTTTTTTAT 63
           GSI +DEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAADG ++         T
Sbjct: 2   GSINKDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAADGSID-----NNIPT 61

Query: 64  LPVVKLTMKPHKANEVGWEAMRWLRGVKGEVGLEHFRLLRRVGSGDIGNVYLCEIRNPMV 123
           LP +++ MKPHKANEVGWEAMRWLR VKGEVGLEHFRLLRRVGSGDIG+VYLCEIRNPMV
Sbjct: 62  LP-LQMMMKPHKANEVGWEAMRWLRRVKGEVGLEHFRLLRRVGSGDIGSVYLCEIRNPMV 121

Query: 124 GLPKCFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLLMEF 183
           GLPKCFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYA FEASHYSCL+MEF
Sbjct: 122 GLPKCFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYADFEASHYSCLVMEF 181

Query: 184 CPGGDLYAARQRQPNKRFSISSAK---------------------DLKPENVLVREDGHI 243
           CPGGDLYAAR R PNKRFSISSAK                     DLKPENVLVREDGHI
Sbjct: 182 CPGGDLYAARLRLPNKRFSISSAKFYAAEIILALEXMHMMGIIYRDLKPENVLVREDGHI 241

Query: 244 MLSDFDLCLKWDVVPKLIRCRTTASDATTERHVRSYS--TSTTSCAAAPIQPVLSCFSIA 303
           MLSDFDLCLK DVVPKLIRCRTTASD   +RHVRSYS  TSTTS   APIQPVLSCFS++
Sbjct: 242 MLSDFDLCLKCDVVPKLIRCRTTASD---DRHVRSYSSTTSTTSAPPAPIQPVLSCFSLS 301

Query: 304 RRKKSTTVTTITEHAAAPSAVYG--GDSDGEGEEFDPEPELIAEPINARSKSFVGTHEYL 363
           RRKKSTTVTTITEHAAAP+ VYG  GDSDG+GEEFDPEPELIAEPINARSKSFVGTHEYL
Sbjct: 302 RRKKSTTVTTITEHAAAPAVVYGGDGDSDGDGEEFDPEPELIAEPINARSKSFVGTHEYL 361

Query: 364 APEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLRFPRIGFSSS 423
           APEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLRFPRIG SSS
Sbjct: 362 APEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLRFPRIGISSS 421

Query: 424 KEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRHDFFKGLNWALIRSVRPPQIPNN- 483
           KEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRH+FFKG+NWALIRSVRPPQIPNN 
Sbjct: 422 KEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRHEFFKGINWALIRSVRPPQIPNNN 481

Query: 484 --NLNYSKYPSNYLPKLSKKQRQQPYQIPNHHFDYF 492
             NLNYSKYPSNYLPKLSKKQRQQP+QIPNHHFDYF
Sbjct: 482 NPNLNYSKYPSNYLPKLSKKQRQQPFQIPNHHFDYF 508

BLAST of Lsi11G003020 vs. NCBI nr
Match: TYJ97801.1 (protein kinase PINOID 2 [Cucumis melo var. makuwa])

HSP 1 Score: 838.6 bits (2165), Expect = 2.8e-239
Identity = 436/520 (83.85%), Postives = 457/520 (87.88%), Query Frame = 0

Query: 2   MAGSIARDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAAD-GGVEMMTTTTT 61
           MAGSI RDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAAD GG+E +T+ TT
Sbjct: 1   MAGSIGRDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAADGGGLENITSGTT 60

Query: 62  TATLPVVKLTMKPHKANEVGWEAMRWLRGVKGEVGLEHFRLLRRVGSGDIGNVYLCEIRN 121
           T T+ V     KPHKANEVGWEAMRWLR  KGEVGLE FRL+RRVGSGDIGNVYLCEIRN
Sbjct: 61  TTTMMV-----KPHKANEVGWEAMRWLRRAKGEVGLEDFRLIRRVGSGDIGNVYLCEIRN 120

Query: 122 PMVGLPKCFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLL 181
           PMVGLPK FYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCL+
Sbjct: 121 PMVGLPKWFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLV 180

Query: 182 MEFCPGGDLYAARQRQPNKRFSISSAK---------------------DLKPENVLVRED 241
           MEFCPGGDLYAAR R PNKRFSI SAK                     DLKPENVLVRED
Sbjct: 181 MEFCPGGDLYAARLRLPNKRFSIPSAKFYAAEIILALEYLHMMGIIYRDLKPENVLVRED 240

Query: 242 GHIMLSDFDLCLKWDVVPKLIRCRTTASDATTERHVRSYS---TSTTSCAAAPIQPVLSC 301
           GHIMLSDFDLCLK DVVPKL+R RTTASD    RH+RS S   ++++SCAAAPIQPVLSC
Sbjct: 241 GHIMLSDFDLCLKCDVVPKLLRSRTTASD----RHLRSSSYTFSTSSSCAAAPIQPVLSC 300

Query: 302 FSIARRKKSTTVTTITEHAAAPSAVYGGDSDGEGEEFDPEPELIAEPINARSKSFVGTHE 361
           FS++RR+KSTTVTTITEHA+   ++YG   D  GEEFDPEPELIAEPINARSKSFVGTHE
Sbjct: 301 FSLSRRQKSTTVTTITEHAS--DSIYGPGDD--GEEFDPEPELIAEPINARSKSFVGTHE 360

Query: 362 YLAPEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLRFPRIGFS 421
           YLAPEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPL+FPRIG S
Sbjct: 361 YLAPEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLKFPRIGIS 420

Query: 422 SSKEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRHDFFKGLNWALIRSVRPPQIP- 481
           SSKEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRH+FFKG+NWALIRSVRPPQIP 
Sbjct: 421 SSKEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRHEFFKGINWALIRSVRPPQIPN 480

Query: 482 ----NNNLNYSKYPSNYLPKLSKKQRQQPYQIPNHHFDYF 492
               NNNLNYSKYPSNYLPKLSKKQRQQPYQIPNHHFDYF
Sbjct: 481 NNIINNNLNYSKYPSNYLPKLSKKQRQQPYQIPNHHFDYF 507

BLAST of Lsi11G003020 vs. NCBI nr
Match: XP_008457762.1 (PREDICTED: protein kinase PINOID 2 [Cucumis melo])

HSP 1 Score: 835.9 bits (2158), Expect = 1.8e-238
Identity = 435/520 (83.65%), Postives = 457/520 (87.88%), Query Frame = 0

Query: 2   MAGSIARDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAAD-GGVEMMTTTTT 61
           MAGSI RDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAAD GG+E +T+ TT
Sbjct: 1   MAGSIGRDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAADGGGLENITSGTT 60

Query: 62  TATLPVVKLTMKPHKANEVGWEAMRWLRGVKGEVGLEHFRLLRRVGSGDIGNVYLCEIRN 121
           T T+ V     KPHKANEVGWEAMRWLR  KGEVGLE FRL+RRVGSGDIGNVYLCEIRN
Sbjct: 61  TTTMMV-----KPHKANEVGWEAMRWLRRAKGEVGLEDFRLIRRVGSGDIGNVYLCEIRN 120

Query: 122 PMVGLPKCFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLL 181
           PMVGLPK FYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCL+
Sbjct: 121 PMVGLPKWFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLV 180

Query: 182 MEFCPGGDLYAARQRQPNKRFSISSAK---------------------DLKPENVLVRED 241
           MEFCPGGDLYAAR R PNKRFSI SAK                     DLKPENVLVRED
Sbjct: 181 MEFCPGGDLYAARLRLPNKRFSIPSAKFYAAEIILALEYLHMMGIIYRDLKPENVLVRED 240

Query: 242 GHIMLSDFDLCLKWDVVPKLIRCRTTASDATTERHVRSYS---TSTTSCAAAPIQPVLSC 301
           GHIMLSDFDLCLK DVVPKL+R RTTASD    RH+RS S   ++++SCAAAPIQPVLSC
Sbjct: 241 GHIMLSDFDLCLKCDVVPKLLRSRTTASD----RHLRSSSYTFSTSSSCAAAPIQPVLSC 300

Query: 302 FSIARRKKSTTVTTITEHAAAPSAVYGGDSDGEGEEFDPEPELIAEPINARSKSFVGTHE 361
           FS++RR+KSTTVTTITE+A+   ++YG   D  GEEFDPEPELIAEPINARSKSFVGTHE
Sbjct: 301 FSLSRRQKSTTVTTITENAS--DSIYGPGDD--GEEFDPEPELIAEPINARSKSFVGTHE 360

Query: 362 YLAPEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLRFPRIGFS 421
           YLAPEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPL+FPRIG S
Sbjct: 361 YLAPEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLKFPRIGIS 420

Query: 422 SSKEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRHDFFKGLNWALIRSVRPPQIP- 481
           SSKEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRH+FFKG+NWALIRSVRPPQIP 
Sbjct: 421 SSKEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRHEFFKGINWALIRSVRPPQIPN 480

Query: 482 ----NNNLNYSKYPSNYLPKLSKKQRQQPYQIPNHHFDYF 492
               NNNLNYSKYPSNYLPKLSKKQRQQPYQIPNHHFDYF
Sbjct: 481 NNIINNNLNYSKYPSNYLPKLSKKQRQQPYQIPNHHFDYF 507

BLAST of Lsi11G003020 vs. NCBI nr
Match: XP_004139320.1 (protein kinase PINOID 2 [Cucumis sativus] >KGN60687.2 hypothetical protein Csa_019374 [Cucumis sativus])

HSP 1 Score: 830.1 bits (2143), Expect = 1.0e-236
Identity = 432/520 (83.08%), Postives = 452/520 (86.92%), Query Frame = 0

Query: 2   MAGSIARDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAAD-GGVEMMTTTTT 61
           MAGSI RDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAAD GG+E +T+ T 
Sbjct: 1   MAGSIGRDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAADGGGLENITSGT- 60

Query: 62  TATLPVVKLTMKPHKANEVGWEAMRWLRGVKGEVGLEHFRLLRRVGSGDIGNVYLCEIRN 121
                   + +KPHKANEVGWEAMRWLR  KGEVGLE FRL+RRVGSGDIGNVYLCEIRN
Sbjct: 61  --------MMVKPHKANEVGWEAMRWLRRAKGEVGLEDFRLIRRVGSGDIGNVYLCEIRN 120

Query: 122 PMVGLPKCFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLL 181
           PMVGLPK FYAMKVVDREAVCVRNKERRADMEKEILK+LDHPFLPTLYAHFEASHYSCL+
Sbjct: 121 PMVGLPKWFYAMKVVDREAVCVRNKERRADMEKEILKLLDHPFLPTLYAHFEASHYSCLV 180

Query: 182 MEFCPGGDLYAARQRQPNKRFSISSAK---------------------DLKPENVLVRED 241
           MEFCPGGDLYAAR R PNKRFSI SAK                     DLKPENVLVRED
Sbjct: 181 MEFCPGGDLYAARLRLPNKRFSIPSAKFYAAEIILALEYLHMMGIIYRDLKPENVLVRED 240

Query: 242 GHIMLSDFDLCLKWDVVPKLIRCRTTASDATTERHVR----SYSTSTTSCAAAPIQPVLS 301
           GHIMLSDFDLCLK DVVPKL+R RTTASD    RHVR    S+STS++SCAAAPIQPVLS
Sbjct: 241 GHIMLSDFDLCLKCDVVPKLLRSRTTASD----RHVRSSSYSFSTSSSSCAAAPIQPVLS 300

Query: 302 CFSIARRKKSTTVTTITEHAAAPSAVYGGDSDGEGEEFDPEPELIAEPINARSKSFVGTH 361
           CFS++RR+KSTTVTTITEHA+        DS   GEEFDPEPELIAEPINARSKSFVGTH
Sbjct: 301 CFSLSRRQKSTTVTTITEHAS--------DSIYSGEEFDPEPELIAEPINARSKSFVGTH 360

Query: 362 EYLAPEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLRFPRIGF 421
           EYLAPEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPL+FPRIG 
Sbjct: 361 EYLAPEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLKFPRIGI 420

Query: 422 SSSKEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRHDFFKGLNWALIRSVRPPQIP 481
           SSSKEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRH+FFKG+NWALIRSVRPPQIP
Sbjct: 421 SSSKEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRHEFFKGINWALIRSVRPPQIP 480

Query: 482 N----NNLNYSKYPSNYLPKLSKKQRQQPYQIPNHHFDYF 492
           N    NNLNYSK+PSNYLPKLSKKQRQQPYQIPNHHFDYF
Sbjct: 481 NNNIINNLNYSKHPSNYLPKLSKKQRQQPYQIPNHHFDYF 499

BLAST of Lsi11G003020 vs. NCBI nr
Match: KAG6586403.1 (Protein kinase PINOID 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 751.9 bits (1940), Expect = 3.5e-213
Identity = 391/495 (78.99%), Postives = 421/495 (85.05%), Query Frame = 0

Query: 5   SIARDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAADGGVEMMTTTTTTATL 64
           SI RD+ SD+DS+ SSS+T+PDSTRSW+SNLS  S RRSS+SA D  +            
Sbjct: 3   SITRDD-SDHDST-SSSLTLPDSTRSWLSNLSISS-RRSSTSALDAALS----------- 62

Query: 65  PVVKLTMKPHKANEVGWEAMRWLRGVKGEVGLEHFRLLRRVGSGDIGNVYLCEIRNPMVG 124
                  KPHKAN V WEAMRW+R VKG VGLEHFRLLRRVGSGDIGNVYLCEIRNP+VG
Sbjct: 63  -----PYKPHKANHVAWEAMRWVRRVKGRVGLEHFRLLRRVGSGDIGNVYLCEIRNPVVG 122

Query: 125 LPKCFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLLMEFC 184
           L +C+YAMKVVDREAV VRNK RRADMEKEILK++DHPFLPTLYA FEASHYSCL+MEFC
Sbjct: 123 LAQCYYAMKVVDREAVSVRNKARRADMEKEILKLIDHPFLPTLYAEFEASHYSCLVMEFC 182

Query: 185 PGGDLYAARQRQPNKRFSISSAKDLKPENVLVREDGHIMLSDFDLCLKWDVVPKLIRCRT 244
           PGGDLYAARQR P KRF ISSAKDLKPENVLVREDGHIMLSDFDLCLK DVVPKLIRCRT
Sbjct: 183 PGGDLYAARQRLPGKRFPISSAKDLKPENVLVREDGHIMLSDFDLCLKCDVVPKLIRCRT 242

Query: 245 TASDATTERHV-RSYST-STTSCAAAPIQPVLSCFSIARRKKSTTVTTITEHAAAPSAVY 304
           TA     ERHV ++YST STTSCAAAPI PVLSCFSI RR  STT+TTITEHAA  +A  
Sbjct: 243 TA-----ERHVTKTYSTSSTTSCAAAPIHPVLSCFSITRRPNSTTITTITEHAAESAATV 302

Query: 305 GGDSDGEGEEFDPEPELIAEPINARSKSFVGTHEYLAPEVISGQGHGSGVDWWTLGIFLY 364
            G       EFDPEPELIAEP+NARSKSFVGTHEYLAPEVISGQGHGS VDWWTLGIFLY
Sbjct: 303 DG-------EFDPEPELIAEPMNARSKSFVGTHEYLAPEVISGQGHGSAVDWWTLGIFLY 362

Query: 365 EMLYGKTPFKGENNEKTLMNILKQPLRFPRIGFSSSKEYEEMVKVEDLIKKLLVKNPKKR 424
           EMLYGKTPFKGENNEKTLMNILKQPL FPRIG SS+KEYEEMVKVEDLI+KLLVKNPKKR
Sbjct: 363 EMLYGKTPFKGENNEKTLMNILKQPLTFPRIGVSSNKEYEEMVKVEDLIRKLLVKNPKKR 422

Query: 425 IGSLKGSVEIKRHDFFKGLNWALIRSVRPPQIPNNN------LNYSKYPSNYLPKLSKKQ 484
           IGSLKGSVEIKRH+FFKG+NWALIRSVRPPQ+PN++      L  SKYPSNY+PKL+KKQ
Sbjct: 423 IGSLKGSVEIKRHEFFKGVNWALIRSVRPPQVPNSDHLLHKTLTQSKYPSNYVPKLTKKQ 466

Query: 485 RQQPYQIPNHHFDYF 492
           RQQPYQIP+HHFDYF
Sbjct: 483 RQQPYQIPSHHFDYF 466

BLAST of Lsi11G003020 vs. TAIR 10
Match: AT2G26700.1 (AGC (cAMP-dependent, cGMP-dependent and protein kinase C) kinase family protein )

HSP 1 Score: 489.6 bits (1259), Expect = 3.0e-138
Identity = 285/536 (53.17%), Postives = 349/536 (65.11%), Query Frame = 0

Query: 11  WSDYDSSCSSSITVPDSTR--SWMSNLSFGSRRRSS-SSAADGGVEMMTTTTTTATLPVV 70
           + D +S   SS   PDS+R  SW+S+    S   SS S  ++ G+     +         
Sbjct: 8   YKDNESDYESSTVGPDSSRRTSWLSSSFTASPSCSSISHLSNHGLNSYNQS--------- 67

Query: 71  KLTMKPHKANEVGWEAMRWLRGVKGE-VGLEHFRLLRRVGSGDIGNVYLCEIRNPMVGLP 130
               KPHKAN+V WEAM  LR   G  VGLEHFRLL+R+GSGDIG+VYLC+IR    G P
Sbjct: 68  ----KPHKANQVAWEAMARLRRCCGRAVGLEHFRLLKRLGSGDIGSVYLCQIR----GSP 127

Query: 131 K-CFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLLMEFCP 190
           +  FYAMKVVD+EAV V+ K  RA+MEK+IL +LDHPF PTLYA FEASHYS L+ME+CP
Sbjct: 128 ETAFYAMKVVDKEAVAVKKKLGRAEMEKKILGMLDHPFCPTLYAAFEASHYSFLVMEYCP 187

Query: 191 GGDLYAARQRQPNKRFSISSA---------------------KDLKPENVLVREDGHIML 250
           GGDLYA R RQP+KRF+ISS                      +DLKPENVL+REDGH+ML
Sbjct: 188 GGDLYAVRLRQPSKRFTISSTRFYAAETLVALEYLHMMGIVYRDLKPENVLIREDGHVML 247

Query: 251 SDFDLCLKWDVVPKLI--RCRTTASDATTERHVRSYSTSTTSCAAAPIQPVLSCF---SI 310
           SDFDL  K DVVP+ +    R        +        ST SC   P+ PV+SCF   S 
Sbjct: 248 SDFDLSFKCDVVPQFLSDNDRDRGHQEDDDDISIRRKCSTPSCTTTPLNPVISCFSPTSS 307

Query: 311 ARRKKSTTVTTITEHAAAPS-AVYGGD---------------SDGEGEEFDPEPELIAEP 370
            RRKK+   TTI E+AA  S +V   D               S+G  +     P + AEP
Sbjct: 308 RRRKKNVVTTTIHENAAGTSDSVKSNDVSRTFSRSPSSCSRVSNGLRDISGGCPSIFAEP 367

Query: 371 INARSKSFVGTHEYLAPEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNI 430
           INARSKSFVGTHEYLAPEVISGQGHGS VDWWT GIFLYEM++G+TPFKG+NNEKTL+NI
Sbjct: 368 INARSKSFVGTHEYLAPEVISGQGHGSAVDWWTYGIFLYEMIFGRTPFKGDNNEKTLVNI 427

Query: 431 LKQPLRFPRIGFSSSKEYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRHDFFKGLNW 490
           LK PL FP++  +S KEYE+MV  +DLI KLLVKNPKKR+GSLKGS+EIKRH+FF+G+NW
Sbjct: 428 LKAPLTFPKVIVNSPKEYEDMVNAQDLIIKLLVKNPKKRLGSLKGSIEIKRHEFFEGVNW 487

Query: 491 ALIRSVRPPQIPNNNLNYSKYPSN-----YLP---KLSKKQRQQPYQIPNHHFDYF 492
           ALIRS++PP +P    ++     N     YLP    +S+K+R +PY + N +FDYF
Sbjct: 488 ALIRSIKPPWVPKEETSHKTKGDNRSVNYYLPPRFMMSRKERNEPYHVSN-YFDYF 525

BLAST of Lsi11G003020 vs. TAIR 10
Match: AT4G26610.1 (D6 protein kinase like 1 )

HSP 1 Score: 374.0 bits (959), Expect = 1.8e-103
Identity = 204/443 (46.05%), Postives = 263/443 (59.37%), Query Frame = 0

Query: 72  KPHKANEVGWEAMRWLRGVKGEVGLEHFRLLRRVGSGDIGNVYLCEIRNPMVGLPKCFYA 131
           KPHKAN+V WEA++ +R   G +GL HFRLL+R+G GDIG V+L E+        +CF+A
Sbjct: 96  KPHKANDVRWEAIQAVRTKHGVLGLNHFRLLKRLGCGDIGTVHLAELHG-----TRCFFA 155

Query: 132 MKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLLMEFCPGGDLYA 191
           MKV+D+ A+  R K  RA  E+EIL+ LDHPFLPTLY+HFE   +SCL+MEFCPGGDL+ 
Sbjct: 156 MKVMDKGALASRKKLLRAQTEREILQCLDHPFLPTLYSHFETEKFSCLVMEFCPGGDLHT 215

Query: 192 ARQRQPNKRFSISSAK---------------------DLKPENVLVREDGHIMLSDFDLC 251
            RQRQP KRFS  +AK                     DLKPENVLVR+DGH+MLSDFDL 
Sbjct: 216 LRQRQPGKRFSEQAAKFYVAEVLLAMEYLHMLGIIYRDLKPENVLVRDDGHVMLSDFDLS 275

Query: 252 LKWDVVPKLIRCRTTASDATTERH--VRSYSTSTTSCAAAPIQPVLSCFSIARRKKSTTV 311
           L+  V P ++R    AS+         +       SC +AP       +  ++ KK   +
Sbjct: 276 LRCTVSPTVVRSTVLASEGQKNSGYCAQPACIQQPSCISAPTTCFSPRYFSSKSKKDKKM 335

Query: 312 TTITEHAAAPSAVYGGDSDGEGEEFDPEPELIAEPINARSKSFVGTHEYLAPEVISGQGH 371
              T                 G +  P PEL+AEP +ARS SFVGTHEYLAPE+I G+GH
Sbjct: 336 KNET-----------------GNQVSPLPELVAEPTSARSMSFVGTHEYLAPEIIKGEGH 395

Query: 372 GSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLRFPRIGFSSSKEYEEMVKVE 431
           GS VDWWT GIFLYE+L+GKTPFKG  N  TL N++ QPLRFP     S           
Sbjct: 396 GSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVVGQPLRFPESPVVS-------FAAR 455

Query: 432 DLIKKLLVKNPKKRIGSLKGSVEIKRHDFFKGLNWALIRSVRPPQIPNNNLNYSKYPSNY 491
           DLI+ LLVK P+ R+   +G+ E+K+H FF+G+NWAL+R   PP+IP   ++Y   P+  
Sbjct: 456 DLIRSLLVKEPQHRLAYKRGATEMKQHPFFEGVNWALVRCASPPEIP-KPVDYESAPAT- 506

BLAST of Lsi11G003020 vs. TAIR 10
Match: AT5G55910.1 (D6 protein kinase )

HSP 1 Score: 369.4 bits (947), Expect = 4.5e-102
Identity = 197/408 (48.28%), Postives = 253/408 (62.01%), Query Frame = 0

Query: 72  KPHKANEVGWEAMRWLRGVKGEVGLEHFRLLRRVGSGDIGNVYLCEIRNPMVGLPKCFYA 131
           KPHKAN+V WEA++ +R   G +GL HFRLL+R+G GDIG V+L E+        +C++A
Sbjct: 82  KPHKANDVRWEAIQAVRTKHGGLGLNHFRLLKRLGCGDIGTVHLAELNG-----TRCYFA 141

Query: 132 MKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLLMEFCPGGDLYA 191
           MKV+D+ A+  R K  RA  E+EIL+ LDHPFLPTLY+HFE   +SCL+MEFCPGGDL+ 
Sbjct: 142 MKVMDKTALASRKKLLRAQTEREILQCLDHPFLPTLYSHFETEKFSCLVMEFCPGGDLHT 201

Query: 192 ARQRQPNKRFSISSAK---------------------DLKPENVLVREDGHIMLSDFDLC 251
            RQRQP KRF+  +AK                     DLKPENVLVR+DGH+MLSDFDL 
Sbjct: 202 LRQRQPGKRFTEQAAKFYVAEVLLAMEYLHMLGIIYRDLKPENVLVRDDGHVMLSDFDLS 261

Query: 252 LKWDVVPKLIRCRTTASDATTERHVRSYSTSTTSCAAAPI---QPVLSCFSIARRKKSTT 311
           L+  V   ++R     S+          S ++ SC+  P    QP  SC S+A     T+
Sbjct: 262 LRCTVSLSIVRSANVGSE--------GLSKNSVSCSQQPACIQQP--SCISMA----PTS 321

Query: 312 VTTITEHAAAPSAVYGGDSDGEGEEFDPEPELIAEPINARSKSFVGTHEYLAPEVISGQG 371
                  ++         ++    +  P PEL+AEP  ARS SFVGTHEYLAPE+I G+G
Sbjct: 322 CFGPRFFSSKSKKDKKPKTENGNHQVTPLPELVAEPTGARSMSFVGTHEYLAPEIIKGEG 381

Query: 372 HGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLRFPRIGFSSSKEYEEMVKV 431
           HGS VDWWT GIFLYE+L+GKTPFKG  N  TL N++ QPLRFP     S          
Sbjct: 382 HGSAVDWWTFGIFLYELLFGKTPFKGSGNRATLFNVVGQPLRFPESPVVS-------FAA 441

Query: 432 EDLIKKLLVKNPKKRIGSLKGSVEIKRHDFFKGLNWALIRSVRPPQIP 456
            DLI+ LLVK P+ R+   +G+ EIK+H FF+G+NWAL+R   PP+IP
Sbjct: 442 RDLIRSLLVKEPQHRLAYKRGATEIKQHPFFEGVNWALVRCASPPEIP 463

BLAST of Lsi11G003020 vs. TAIR 10
Match: AT5G47750.1 (D6 protein kinase like 2 )

HSP 1 Score: 360.5 bits (924), Expect = 2.1e-99
Identity = 209/476 (43.91%), Postives = 275/476 (57.77%), Query Frame = 0

Query: 4   GSIARDEWSDYDSSCSSSITVPDSTRSWMSNLSFGSRRRSSSSAADGGVEMMTTTTTTAT 63
           GS+     S   S  +SS+     +      L F    +SS        ++   ++ ++ 
Sbjct: 99  GSVKTSSASAKLSDGTSSLGKTSGSAKLSGRLDFMESGKSSICRGSTSSDVSDESSCSSF 158

Query: 64  LPVVKLTMKPHKANEVGWEAMRWLRGVKGEVGLEHFRLLRRVGSGDIGNVYLCEIRNPMV 123
              V    KPHKAN++ WEA++ +R   G +GL HFRLL+R+G GDIG+VYL E+     
Sbjct: 159 SSTVN---KPHKANDLRWEAIQAVRVRDGLLGLSHFRLLKRLGCGDIGSVYLSELSG--- 218

Query: 124 GLPKCFYAMKVVDREAVCVRNKERRADMEKEILKVLDHPFLPTLYAHFEASHYSCLLMEF 183
              KC++AMKV+D+ ++  R K  RA  E+EIL+ LDHPFLPTLY HFE   +SCL+MEF
Sbjct: 219 --TKCYFAMKVMDKTSLASRKKLLRAQTEREILQSLDHPFLPTLYTHFETEKFSCLVMEF 278

Query: 184 CPGGDLYAARQRQPNKRFSISSAK---------------------DLKPENVLVREDGHI 243
           CPGGDL+  RQRQP K FS  + K                     DLKPENVLVREDGHI
Sbjct: 279 CPGGDLHTLRQRQPGKHFSEQAVKFYIAESLLALEYLHMLGIVYRDLKPENVLVREDGHI 338

Query: 244 MLSDFDLCLKWDVVPKLIRCRTTASDATTERHVRSYSTSTTSCAAAPIQPVLSCFSIARR 303
           MLSDFDL L+  V P L++     SD   +            C    +QP  +C   +  
Sbjct: 339 MLSDFDLSLRCLVSPTLVKSAAIESDPLRK---------NVYC----VQP--ACIEPSCI 398

Query: 304 KKSTTVTTITEHAAAPSAVYGGD---SDGEGEEFDPEPELIAEPINARSKSFVGTHEYLA 363
           + S TV T        S+    D    +    +  P PEL+AEP +ARS SFVGTHEYLA
Sbjct: 399 QPSCTVPTTCFSPRLFSSKSKKDRKPKNDTANQVRPLPELVAEPTDARSMSFVGTHEYLA 458

Query: 364 PEVISGQGHGSGVDWWTLGIFLYEMLYGKTPFKGENNEKTLMNILKQPLRFPRIGFSSSK 423
           PE+I G+GHGS VDWWT GIFLYE+L+G+TPFKG  N +TL N++ QPLRFP     S  
Sbjct: 459 PEIIKGEGHGSAVDWWTFGIFLYELLFGRTPFKGSGNRQTLFNVVGQPLRFPETPVVS-- 518

Query: 424 EYEEMVKVEDLIKKLLVKNPKKRIGSLKGSVEIKRHDFFKGLNWALIRSVRPPQIP 456
                    DLI+ LL+K P++R+G  +G+ E+K+H FF+G+NWALIR   PP+IP
Sbjct: 519 -----FAARDLIRGLLMKEPQQRLGFKRGATEVKQHPFFEGVNWALIRCATPPEIP 544

BLAST of Lsi11G003020 vs. TAIR 10
Match: AT5G40030.1 (Protein kinase superfamily protein )

HSP 1 Score: 349.4 bits (895), Expect = 4.8e-96
Identity = 213/451 (47.23%), Postives = 267/451 (59.20%), Query Frame = 0

Query: 34  NLSFGSRRRSSSSAADGGVE-MMTTTTTTATLPVVKLTMKPHKANEVGWEAMRWLRGVKG 93
           +LSF S   S+S +++  +E   +     A    V    KPHK N++ W+A++ ++  K 
Sbjct: 50  SLSFCS---SNSVSSEANLEKTQSFDANEANFKRVFAPSKPHKGNDLRWDAIQNVKCSKN 109

Query: 94  E-VGLEHFRLLRRVGSGDIGNVYLCEIRNPMVGLPKCFYAMKVVDREAVCVRNKERRADM 153
           E +GL HFRLL+++G GDIG+VYL E+R        CF+AMKV+D+  +  R K  RA  
Sbjct: 110 EDLGLGHFRLLKKLGCGDIGSVYLAELRE-----MGCFFAMKVMDKGMLIGRKKLVRAQT 169

Query: 154 EKEILKVLDHPFLPTLYAHFEASHYSCLLMEFCPGGDLYAARQRQPNKRFSISSA----- 213
           E+EIL +LDHPFLPTLY+HFE   +SCLLMEFC GGDL+  RQ+QP K FS  +A     
Sbjct: 170 EREILGLLDHPFLPTLYSHFETEKFSCLLMEFCSGGDLHILRQKQPGKHFSELAARFYAS 229

Query: 214 ----------------KDLKPENVLVREDGHIMLSDFDLCLKWDVVPKLIRCRTTASDAT 273
                           +DLKPENV+VREDGHIMLSDFDL L+  V P LI+     S + 
Sbjct: 230 EVLLALEYLHMMGVVYRDLKPENVMVREDGHIMLSDFDLSLQSFVSPTLIQ-----STSQ 289

Query: 274 TERHVRSYSTS----TTSC--AAAPIQPVLSCFSIARRKKSTTVTTITEHAAAPSAVYGG 333
              H+ SY         SC    A IQP  SCF   R   +      TE A +       
Sbjct: 290 PSCHIASYCIQPPCIDPSCKLPVACIQP--SCFK-PRFLNNKPRKAKTEKAGS------- 349

Query: 334 DSDGEGEEFDPEPELIAEPINARSKSFVGTHEYLAPEVISGQGHGSGVDWWTLGIFLYEM 393
                    D  P LIAEP  ARS SFVGTHEYLAPE+I G GHGS VDWWT GIFLYE+
Sbjct: 350 ---------DSLPMLIAEPTAARSMSFVGTHEYLAPEIIRGDGHGSSVDWWTFGIFLYEL 409

Query: 394 LYGKTPFKGENNEKTLMNILKQPLRFPRIGFSSSKEYEEMVKVEDLIKKLLVKNPKKRIG 453
           L GKTPFKG  N +TL N++ QPL+FP    S +         +DLI+ LL K+PKKR+G
Sbjct: 410 LTGKTPFKGNGNRETLFNVVGQPLKFPEGSISFA--------AKDLIRGLLTKDPKKRLG 460

Query: 454 SLKGSVEIKRHDFFKGLNWALIRSVRPPQIP 456
             KG+ EIK+H FF  +NWALIRS  PP+IP
Sbjct: 470 FKKGATEIKQHPFFNNVNWALIRSTTPPEIP 460

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94E493.4e-13953.88Protein kinase PINOID 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PID2 PE=2 SV... [more]
Q64FQ24.2e-13753.17Protein kinase PINOID 2 OS=Arabidopsis thaliana OX=3702 GN=PID2 PE=1 SV=1[more]
Q9SUA32.6e-10246.05Serine/threonine-protein kinase D6PKL1 OS=Arabidopsis thaliana OX=3702 GN=D6PKL1... [more]
Q9FG746.3e-10148.28Serine/threonine-protein kinase D6PK OS=Arabidopsis thaliana OX=3702 GN=D6PK PE=... [more]
A2YBX52.3e-9845.70Protein kinase G11A OS=Oryza sativa subsp. indica OX=39946 GN=OsI_021818 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A5D3BGM81.4e-23983.85Protein kinase PINOID 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3C6E48.8e-23983.65protein kinase PINOID 2 OS=Cucumis melo OX=3656 GN=LOC103497373 PE=4 SV=1[more]
A0A6J1HPF11.7e-21075.72protein kinase PINOID 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465514 PE=4 SV... [more]
A0A6J1FGS32.1e-20875.39protein kinase PINOID 2-like OS=Cucurbita moschata OX=3662 GN=LOC111443882 PE=4 ... [more]
A0A6J1KGU74.7e-20877.39protein kinase PINOID 2-like OS=Cucurbita maxima OX=3661 GN=LOC111493762 PE=4 SV... [more]
Match NameE-valueIdentityDescription
XP_038889440.15.7e-24886.43LOW QUALITY PROTEIN: protein kinase PINOID 2 [Benincasa hispida][more]
TYJ97801.12.8e-23983.85protein kinase PINOID 2 [Cucumis melo var. makuwa][more]
XP_008457762.11.8e-23883.65PREDICTED: protein kinase PINOID 2 [Cucumis melo][more]
XP_004139320.11.0e-23683.08protein kinase PINOID 2 [Cucumis sativus] >KGN60687.2 hypothetical protein Csa_0... [more]
KAG6586403.13.5e-21378.99Protein kinase PINOID 2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT2G26700.13.0e-13853.17AGC (cAMP-dependent, cGMP-dependent and protein kinase C) kinase family protein [more]
AT4G26610.11.8e-10346.05D6 protein kinase like 1 [more]
AT5G55910.14.5e-10248.28D6 protein kinase [more]
AT5G47750.12.1e-9943.91D6 protein kinase like 2 [more]
AT5G40030.14.8e-9647.23Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 99..438
e-value: 6.7E-52
score: 188.4
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 100..195
e-value: 1.5E-9
score: 37.7
coord: 328..438
e-value: 4.9E-22
score: 78.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 99..438
score: 28.993793
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 211..296
e-value: 7.1E-6
score: 27.6
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 326..456
e-value: 7.1E-38
score: 132.2
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 51..210
e-value: 1.3E-23
score: 85.1
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 93..199
e-value: 0.36
score: 7.6
NoneNo IPR availablePIRSRPIRSR037014-2PIRSR037014-2coord: 331..453
e-value: 1.8E-8
score: 31.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availablePANTHERPTHR45637FLIPPASE KINASE 1-RELATEDcoord: 207..491
coord: 7..207
NoneNo IPR availablePANTHERPTHR45637:SF12PROTEIN KINASE PINOID 2coord: 207..491
coord: 7..207
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 81..474

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi11G003020.1Lsi11G003020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity