Lsi11G001980 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi11G001980
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPhospholipase A2 family protein
Locationchr11: 2026072 .. 2029076 (+)
RNA-Seq ExpressionLsi11G001980
SyntenyLsi11G001980
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCCGCAACACTAGCATTTCCATTCCCAAACATCTCTCATCCGCCATTCTCCTTCTCCTCCTTCTTCTCGCCGTCGTCTCCGACTGTTCCAACAACGAATCCCGGGTAATAATCTCAACCTTTCGTGTTTTCAATAACTAAATGCTGGAGCATTCGAATCTTAGATTCGTACATTTGGATCTTCAATTTCCTTCCTTTCTTTCAATGTCACAACCTTCGCGTAATGTGAAATTCAGTTTGTTTGGTTTCTGTTCAACGATTTCGATGTTAACGTCAATATTTCGTTGGATGATAGGTTGAATGCAGCAGAACTTGTGTTGCAATTAACTGCAATAGTGAGTGTATTTTGGAATTTATGTGAACTTCGTCTTCTGTTATTACTGTTTTTTGAATTTTAGTTTCTTCCTTGCATATTTATTTTGGTCGTTTATCTGAAATTTAGCTGTTGGGATTCGGTACGGAAAGTTCTGCGGAGTAGGATGGACGGGTTGTGCTGGTGAAAAGCCTTGCGATGATCTTGATGCCTGTTGCAAAGTTCATGACGAATGTGTTGAAAGAAAAGGTTATTGCCAATCTATAACTACATTACCTAATTAATCTGTCCTTTGTTTACTTCGCATTGTTTGCCATCGATCTATAATCCTGTTTTACTCTTTAGTTGTTGTGAGGTCTAATCGGGTGAAATCCGGGCATCCTCTTGTTGCATCAACACCTTCTTTTTTTTTTCTTTCTTTTGGTGTTGGGGGGGGGGGGGGGGGGGGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGGGGGGGGGGGGGGGGGGGGGGCGCATTATTGGTATGTTCTCTGCTGCATACCTTGTAAAGATACGCATGAGATTGAAATTTCAACTACAATGTCTATCCCTTGAGAATGAAGGGAGCAGCAACTAGTCATCAACAACCCAAAAAATTGGGTGTTAGTATCCACGATTAACGGGTGGGCACCCCCCTCGTTTTCGTGCTCCTTAGATCATTTCCGATTTCAAATAAAATTCCCGTTGAATTCTTCATCCTCAGCACCTAGGTTAAACTGAGCAATTGCAAAATTCTAGAATTTAGGAGCGTATATCACTTGTTAAGGACAACTAATGGTTAACATATCTACGATGGGATGATGGTGGCCACTTTGGTCATAAGCCCAAATGATTCTGTTTTTTTATTACACCCAAAATACCACATACTAATGGTGATGGTTGTCCTCATATGTGGCCATGATCTTCTCCTTCCATAGTCAATGTCAGACTTTGGTCGCATTCCTAACTATACTCTCCTCAAACAAAGTACTATTGAGCCTCCCTGTTCTAAGACTCATCACTTCTTTGTTTGGCACCTGAACTCTATTAGTCTGCCTAAGTCAATGGCGTGACTCTGGTACTATAACAAGAATTAGAAAGAGAGAGAAGAGTGAATAACAGTGATCATAGAAGTATTATGCTATCCAATGGAGTTAGAAAACATTATTTTCATAATTAGAATTCTCTCTCAACTTGTCTCATCAGGTACAGTTGTAACAAGATCTTAACAATCAATGAGCTCTCTTAAGTCTTAGCACTGTTGCTGTGTTAGCCACACTAATAATGGAAGAAAGGGTAATGAGTTGCTCTGTCATATGCAATGACCAGTGACGCTTTGATTCCATTCTGCTAAAATCACTGAAGGAGAAAACAGTATCCCACTAACTTTTATGTCCGCTCACTTATTAGTTATGTTCACTATATTGAGCAAGTGAGACCAGTTCTGGACACTTGTCCTTGTGAATGGTGAAAGTAGTCTCGTAAAATTAAATTAATATAAAATTAATTGTTACGTGTACTTAGCAGCAAAATATTCTTTGAGTCCCGAAAGTTCTACTGTTATGTCAGATTCGTGATGCCACAAGGTTAATGTGCTTCCTGGCATGGAGAGGCTGATTTTCATCCTCAAAATGCCTGTGCGGGCTATAATGGACCACTCCCGTTTGTATATGGATTTTTTTGACAGACGACAAAGCATGGTTGAAATAGTCTATTATGTTCTTCACAACTTTGAAGTAGAATCCTTCGTGCCATTAACTTTGGTTTAGGATGACCAAGCGTCACATATTATTCCCTTTTCAAATGCATGTTTGCAGTAAGGACGTTGAACACTTGTTCCTCCATTGTAGTGATCATTGAGTGGATTACATCAGTAAAAGCCTATGAATAAGTCAGTTATTTATATTATGCACATAGTAGACTTCTGTTTATTACGGATCAGTTAAGCTATCGGCCTACTGGCAATTGAAAAACAAAACTAGATACAAAATTGGAGGATATTTGAGGCATGGCCAATTGGCATGTTAAACACAGAGGAGACACTAGACCAATGTCTGGTGTTGGTAAATTCTAGATCTTAGATCTTAGATGCTTGTGATTTATCTCAGATACTTCATATTTGAAACTTGTAGCATTAGATTAGAGAAGGAGACCAAGCAATTTATATATATTGGTCTATCCTGTTTTGTTTTGTTTCTGATTTGTGTGGAATTATAACAGTTGGAACTTGGGAGTCACTCTGTTTAATATGTTTCAAGACCAATAACCTCTATCACATGGGTTTTTCAGGTTTAACTAATATTAAATGTCACGAGAAGTTCAAGACTTGTATAAAGAGAGTGCAGAAATCTGGGAAGGTTGGTTTCTCACAGGAGTGCCCTTATTCCACAGCTGTTCCTACAATGGTGCAGGGCATGGATTTGGCTATCATGTTCAGCCAATTTGGTAACTCAAAGCTTGAGCTCTGA

mRNA sequence

ATGGCCCGCAACACTAGCATTTCCATTCCCAAACATCTCTCATCCGCCATTCTCCTTCTCCTCCTTCTTCTCGCCGTCGTCTCCGACTGTTCCAACAACGAATCCCGGGTTGAATGCAGCAGAACTTGTGTTGCAATTAACTGCAATACTGTTGGGATTCGGTACGGAAAGTTCTGCGGAGTAGGATGGACGGGTTGTGCTGGTGAAAAGCCTTGCGATGATCTTGATGCCTGTTGCAAAGTTCATGACGAATGTGTTGAAAGAAAAGGTTTAACTAATATTAAATGTCACGAGAAGTTCAAGACTTGTATAAAGAGAGTGCAGAAATCTGGGAAGGTTGGTTTCTCACAGGAGTGCCCTTATTCCACAGCTGTTCCTACAATGGTGCAGGGCATGGATTTGGCTATCATGTTCAGCCAATTTGGTAACTCAAAGCTTGAGCTCTGA

Coding sequence (CDS)

ATGGCCCGCAACACTAGCATTTCCATTCCCAAACATCTCTCATCCGCCATTCTCCTTCTCCTCCTTCTTCTCGCCGTCGTCTCCGACTGTTCCAACAACGAATCCCGGGTTGAATGCAGCAGAACTTGTGTTGCAATTAACTGCAATACTGTTGGGATTCGGTACGGAAAGTTCTGCGGAGTAGGATGGACGGGTTGTGCTGGTGAAAAGCCTTGCGATGATCTTGATGCCTGTTGCAAAGTTCATGACGAATGTGTTGAAAGAAAAGGTTTAACTAATATTAAATGTCACGAGAAGTTCAAGACTTGTATAAAGAGAGTGCAGAAATCTGGGAAGGTTGGTTTCTCACAGGAGTGCCCTTATTCCACAGCTGTTCCTACAATGGTGCAGGGCATGGATTTGGCTATCATGTTCAGCCAATTTGGTAACTCAAAGCTTGAGCTCTGA

Protein sequence

MARNTSISIPKHLSSAILLLLLLLAVVSDCSNNESRVECSRTCVAINCNTVGIRYGKFCGVGWTGCAGEKPCDDLDACCKVHDECVERKGLTNIKCHEKFKTCIKRVQKSGKVGFSQECPYSTAVPTMVQGMDLAIMFSQFGNSKLEL
Homology
BLAST of Lsi11G001980 vs. ExPASy Swiss-Prot
Match: Q9XG80 (Probable phospholipase A2 homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-I PE=2 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 8.7e-46
Identity = 79/134 (58.96%), Postives = 105/134 (78.36%), Query Frame = 0

Query: 15  SAILLLLLLLAVVSDCSNNESRVECSRTCVAINCNTVGIRYGKFCGVGWTGCAGEKPCDD 74
           S +L L+ L A V   + +     CSR+C A+NC++VGIRYGK+CGVGW+GC GE+PCDD
Sbjct: 5   SPLLALVFLAAGVLSSATSPPPPPCSRSCAALNCDSVGIRYGKYCGVGWSGCDGEEPCDD 64

Query: 75  LDACCKVHDECVERKGLTNIKCHEKFKTCIKRVQKSGKVGFSQECPYSTAVPTMVQGMDL 134
           LDACC+ HD CV++KGL ++KCHEKFK C+++V+K+GK+GFS++CPY  A+ TM  GMD+
Sbjct: 65  LDACCRDHDHCVDKKGLMSVKCHEKFKNCMRKVKKAGKIGFSRKCPYEMAMATMTSGMDM 124

Query: 135 AIMFSQFGNSKLEL 149
           AIM SQ G  KLEL
Sbjct: 125 AIMLSQLGTQKLEL 138

BLAST of Lsi11G001980 vs. ExPASy Swiss-Prot
Match: Q8GZB4 (Phospholipase A2-beta OS=Arabidopsis thaliana OX=3702 GN=PLA2-BETA PE=1 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 7.2e-40
Identity = 71/118 (60.17%), Postives = 91/118 (77.12%), Query Frame = 0

Query: 38  ECSRTCVAINCNTVGIRYGKFCGVGWTGCAGEKPCDDLDACCKVHDECVERKGLTNIKCH 97
           EC+RTC+A NC+T+ IRYGK+CG+G +GC GE+PCDDLDACCK+HD CVE  G+TNI CH
Sbjct: 30  ECTRTCIAQNCDTLSIRYGKYCGIGHSGCPGEEPCDDLDACCKIHDHCVELNGMTNISCH 89

Query: 98  EKFKTCIKRV------QKSGKVGFSQECPYSTAVPTMVQGMDLAIMFSQFGNS-KLEL 149
           +KF+ C+ R+       K+ KVGFS +CPYS  +PT+ QGMD+ I+FSQ GN  K EL
Sbjct: 90  KKFQRCVNRLSKAIKQSKNKKVGFSTKCPYSVVIPTVNQGMDIGILFSQLGNDMKTEL 147

BLAST of Lsi11G001980 vs. ExPASy Swiss-Prot
Match: Q9M0D7 (Phospholipase A2-gamma OS=Arabidopsis thaliana OX=3702 GN=PLA2-GAMMA PE=1 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 4.3e-37
Identity = 71/132 (53.79%), Postives = 91/132 (68.94%), Query Frame = 0

Query: 18  LLLLLLLAVVSDCSNNESRVECSRTCVAINCNTVGIRYGKFCGVGWTGCAGEKPCDDLDA 77
           L   LLLAVVS      S+ +CS TC+A NCN++GIRYGK+CG+G+ GC GE PCDDLDA
Sbjct: 14  LTAFLLLAVVS------SQEKCSNTCIAQNCNSLGIRYGKYCGIGYFGCPGEPPCDDLDA 73

Query: 78  CCKVHDECVERKGLTNIKCHEKFKTCIKRVQKS------GKVGFSQECPYSTAVPTMVQG 137
           CC  HD CV+ KG+T + CH++FK C+ ++ KS       K+GFS +CPYS  +PT+  G
Sbjct: 74  CCMTHDNCVDLKGMTYVNCHKQFKRCVNKLSKSIKHSNGEKIGFSTQCPYSIVIPTVFNG 133

Query: 138 MDLAIMFSQFGN 144
           MD  I FS  GN
Sbjct: 134 MDYGIFFSGIGN 139

BLAST of Lsi11G001980 vs. ExPASy Swiss-Prot
Match: Q8GV50 (Phospholipase A2-delta OS=Arabidopsis thaliana OX=3702 GN=PLA2-DELTA PE=2 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 1.8e-35
Identity = 68/132 (51.52%), Postives = 92/132 (69.70%), Query Frame = 0

Query: 18  LLLLLLLAVVSDCSNNESRVECSRTCVAINCNTVGIRYGKFCGVGWTGCAGEKPCDDLDA 77
           L + LLLAVV       S+ +CS+TC+A  CN +GIRYGK+CG+G+ GC GE PCDDLD 
Sbjct: 14  LTVFLLLAVV------HSQEKCSKTCIAQKCNVLGIRYGKYCGIGYFGCPGEPPCDDLDD 73

Query: 78  CCKVHDECVERKGLTNIKCHEKFKTCIKRVQKS------GKVGFSQECPYSTAVPTMVQG 137
           CC  HD CV+ KG+T + CH++F+ C+  +++S       KVGFS+ECPYST +PT+ +G
Sbjct: 74  CCMTHDNCVDLKGMTYVDCHKQFQRCVNELKQSIQESNNQKVGFSKECPYSTVIPTVYRG 133

Query: 138 MDLAIMFSQFGN 144
           M+  I FS  GN
Sbjct: 134 MNYGIFFSGIGN 139

BLAST of Lsi11G001980 vs. ExPASy Swiss-Prot
Match: Q10E50 (Phospholipase A2 homolog 3 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-III PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 4.7e-15
Identity = 36/101 (35.64%), Postives = 60/101 (59.41%), Query Frame = 0

Query: 39  CSRTCVAINCNTVG-IRYGKFCGVGWTGCAGEKPCDDLDACCKVHDECVERKG-LTNIKC 98
           CSRTC + +C T   +RYGK+CG+ ++GC GE+PCD+LDACC  HD CV+ K    +  C
Sbjct: 55  CSRTCESDHCTTPPLLRYGKYCGILYSGCPGEQPCDELDACCMHHDNCVQAKNDYLSTAC 114

Query: 99  HEKFKTCIKRVQKSGKVGFSQECPYSTAVPTMVQGMDLAIM 138
           +E+   C+ R+++        +C     +  +   ++ A++
Sbjct: 115 NEELLECLARLREGSSTFQGNKCMIDEVIDVISLVIEAAVV 155

BLAST of Lsi11G001980 vs. ExPASy TrEMBL
Match: A0A1S3C986 (probable phospholipase A2 homolog 1 OS=Cucumis melo OX=3656 GN=LOC103498425 PE=4 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.1e-70
Identity = 133/148 (89.86%), Postives = 138/148 (93.24%), Query Frame = 0

Query: 1   MARNTSISIPKHLSSAILLLLLLLAVVSDCSNNESRVECSRTCVAINCNTVGIRYGKFCG 60
           MARNT IS        ILLLLLLL VVSDCSNNESRVECSRTCVA+NCNTVGIRYGKFCG
Sbjct: 1   MARNTIIS--------ILLLLLLLTVVSDCSNNESRVECSRTCVAVNCNTVGIRYGKFCG 60

Query: 61  VGWTGCAGEKPCDDLDACCKVHDECVERKGLTNIKCHEKFKTCIKRVQKSGKVGFSQECP 120
           VGWTGCAGEKPCDDLDACCKVHDECVERKGLTN+KCHEKFK+CIK+VQKSGKVGFS+ECP
Sbjct: 61  VGWTGCAGEKPCDDLDACCKVHDECVERKGLTNVKCHEKFKSCIKKVQKSGKVGFSKECP 120

Query: 121 YSTAVPTMVQGMDLAIMFSQFGNSKLEL 149
           YSTAVPTMVQGMDLAIMFSQFGNSKLEL
Sbjct: 121 YSTAVPTMVQGMDLAIMFSQFGNSKLEL 140

BLAST of Lsi11G001980 vs. ExPASy TrEMBL
Match: A0A6J1DMI5 (probable phospholipase A2 homolog 1 OS=Momordica charantia OX=3673 GN=LOC111021924 PE=4 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 1.5e-69
Identity = 128/148 (86.49%), Postives = 137/148 (92.57%), Query Frame = 0

Query: 1   MARNTSISIPKHLSSAILLLLLLLAVVSDCSNNESRVECSRTCVAINCNTVGIRYGKFCG 60
           M R+ SI I KH SS  LL+LL L+VVS+CS+NESRVECSRTCVAINCNTVGIRYGKFCG
Sbjct: 1   MVRHPSIPIRKHFSSPFLLVLLFLSVVSECSSNESRVECSRTCVAINCNTVGIRYGKFCG 60

Query: 61  VGWTGCAGEKPCDDLDACCKVHDECVERKGLTNIKCHEKFKTCIKRVQKSGKVGFSQECP 120
           VGWTGCAGEKPCDDLDACCKVHDECVERKGLTNIKCHEKFK+CIK+VQKSGK GFS+ECP
Sbjct: 61  VGWTGCAGEKPCDDLDACCKVHDECVERKGLTNIKCHEKFKSCIKKVQKSGKAGFSEECP 120

Query: 121 YSTAVPTMVQGMDLAIMFSQFGNSKLEL 149
           YSTAVP MVQGMDLAI+FSQ GNSKLEL
Sbjct: 121 YSTAVPIMVQGMDLAIVFSQLGNSKLEL 148

BLAST of Lsi11G001980 vs. ExPASy TrEMBL
Match: A0A0A0LFR9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000560 PE=4 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.7e-68
Identity = 130/148 (87.84%), Postives = 136/148 (91.89%), Query Frame = 0

Query: 1   MARNTSISIPKHLSSAILLLLLLLAVVSDCSNNESRVECSRTCVAINCNTVGIRYGKFCG 60
           MARNT IS        ILLLLLLL VVS CSNNESRVECSRTCVAINCNTVGIRYGKFCG
Sbjct: 1   MARNTIIS--------ILLLLLLLTVVSHCSNNESRVECSRTCVAINCNTVGIRYGKFCG 60

Query: 61  VGWTGCAGEKPCDDLDACCKVHDECVERKGLTNIKCHEKFKTCIKRVQKSGKVGFSQECP 120
           VGWTGCAGEKPCDDLDACCKVHDECVERKGLTN+KCHEKFK+CIK+VQKSGKVGFS +CP
Sbjct: 61  VGWTGCAGEKPCDDLDACCKVHDECVERKGLTNVKCHEKFKSCIKKVQKSGKVGFSNDCP 120

Query: 121 YSTAVPTMVQGMDLAIMFSQFGNSKLEL 149
           YSTAVPTMVQGM+LAIMFS+FGNSKLEL
Sbjct: 121 YSTAVPTMVQGMNLAIMFSKFGNSKLEL 140

BLAST of Lsi11G001980 vs. ExPASy TrEMBL
Match: A0A6J1KDD2 (probable phospholipase A2 homolog 1 OS=Cucurbita maxima OX=3661 GN=LOC111493870 PE=4 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 4.2e-59
Identity = 113/148 (76.35%), Postives = 127/148 (85.81%), Query Frame = 0

Query: 1   MARNTSISIPKHLSSAILLLLLLLAVVSDCSNNESRVECSRTCVAINCNTVGIRYGKFCG 60
           M  NTSIS+ KH++SAI LLL+LL+VVS+CS   S+VECSRTCV+  CNTVGIRYGKFCG
Sbjct: 1   MVCNTSISLRKHVASAIFLLLVLLSVVSECS---SQVECSRTCVSEKCNTVGIRYGKFCG 60

Query: 61  VGWTGCAGEKPCDDLDACCKVHDECVERKGLTNIKCHEKFKTCIKRVQKSGKVGFSQECP 120
           VGWTGCAGEKPCDDLDACCK HDECVE KGLTNIKCH KFK CI ++QKSG  GFSQ+CP
Sbjct: 61  VGWTGCAGEKPCDDLDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCP 120

Query: 121 YSTAVPTMVQGMDLAIMFSQFGNSKLEL 149
           YS A+PTMV GMD+AIMFSQF + K EL
Sbjct: 121 YSIAIPTMVNGMDIAIMFSQFSSGKFEL 145

BLAST of Lsi11G001980 vs. ExPASy TrEMBL
Match: W9QS24 (Putative phospholipase A2-1-like protein OS=Morus notabilis OX=981085 GN=L484_027241 PE=4 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 6.0e-58
Identity = 104/142 (73.24%), Postives = 122/142 (85.92%), Query Frame = 0

Query: 7   ISIPKHLSSAILLLLLLLAVVSDCSNNESRVECSRTCVAINCNTVGIRYGKFCGVGWTGC 66
           + +   +++   ++L+ L VV+ CSNN+S V CS+ CVA NCN+VGIRYGKFCGVGWTGC
Sbjct: 8   VPVRTRVAAGFAVVLISLTVVAHCSNNDSEVRCSKICVAENCNSVGIRYGKFCGVGWTGC 67

Query: 67  AGEKPCDDLDACCKVHDECVERKGLTNIKCHEKFKTCIKRVQKSGKVGFSQECPYSTAVP 126
            GEKPCDDLDACCK+HDECV +KGLTN+KCHEKFK+CIKRVQKSGKVGFS+ CPY TAVP
Sbjct: 68  PGEKPCDDLDACCKIHDECVTKKGLTNVKCHEKFKSCIKRVQKSGKVGFSRVCPYKTAVP 127

Query: 127 TMVQGMDLAIMFSQFGNSKLEL 149
           TMVQGMDLAI+ SQFGNSK EL
Sbjct: 128 TMVQGMDLAILLSQFGNSKAEL 149

BLAST of Lsi11G001980 vs. NCBI nr
Match: XP_038889610.1 (probable phospholipase A2 homolog 1 [Benincasa hispida])

HSP 1 Score: 295.0 bits (754), Expect = 3.5e-76
Identity = 142/148 (95.95%), Postives = 145/148 (97.97%), Query Frame = 0

Query: 1   MARNTSISIPKHLSSAILLLLLLLAVVSDCSNNESRVECSRTCVAINCNTVGIRYGKFCG 60
           MA NTSISI KHLSSAILLL+LLL VVSDCSNNESRVECSRTCVAINCNTVGIRYGKFCG
Sbjct: 1   MAGNTSISILKHLSSAILLLILLLTVVSDCSNNESRVECSRTCVAINCNTVGIRYGKFCG 60

Query: 61  VGWTGCAGEKPCDDLDACCKVHDECVERKGLTNIKCHEKFKTCIKRVQKSGKVGFSQECP 120
           VGWTGCAGEKPCDDLDACCKVHDECVERKGLTNIKCHEKFK+CIKRV+KSGKVGFSQECP
Sbjct: 61  VGWTGCAGEKPCDDLDACCKVHDECVERKGLTNIKCHEKFKSCIKRVRKSGKVGFSQECP 120

Query: 121 YSTAVPTMVQGMDLAIMFSQFGNSKLEL 149
           YSTAVPTMVQGMDLAIMFSQFGNSKLEL
Sbjct: 121 YSTAVPTMVQGMDLAIMFSQFGNSKLEL 148

BLAST of Lsi11G001980 vs. NCBI nr
Match: XP_008459246.1 (PREDICTED: probable phospholipase A2 homolog 1 [Cucumis melo])

HSP 1 Score: 275.8 bits (704), Expect = 2.2e-70
Identity = 133/148 (89.86%), Postives = 138/148 (93.24%), Query Frame = 0

Query: 1   MARNTSISIPKHLSSAILLLLLLLAVVSDCSNNESRVECSRTCVAINCNTVGIRYGKFCG 60
           MARNT IS        ILLLLLLL VVSDCSNNESRVECSRTCVA+NCNTVGIRYGKFCG
Sbjct: 1   MARNTIIS--------ILLLLLLLTVVSDCSNNESRVECSRTCVAVNCNTVGIRYGKFCG 60

Query: 61  VGWTGCAGEKPCDDLDACCKVHDECVERKGLTNIKCHEKFKTCIKRVQKSGKVGFSQECP 120
           VGWTGCAGEKPCDDLDACCKVHDECVERKGLTN+KCHEKFK+CIK+VQKSGKVGFS+ECP
Sbjct: 61  VGWTGCAGEKPCDDLDACCKVHDECVERKGLTNVKCHEKFKSCIKKVQKSGKVGFSKECP 120

Query: 121 YSTAVPTMVQGMDLAIMFSQFGNSKLEL 149
           YSTAVPTMVQGMDLAIMFSQFGNSKLEL
Sbjct: 121 YSTAVPTMVQGMDLAIMFSQFGNSKLEL 140

BLAST of Lsi11G001980 vs. NCBI nr
Match: XP_022154742.1 (probable phospholipase A2 homolog 1 [Momordica charantia] >XP_022154743.1 probable phospholipase A2 homolog 1 [Momordica charantia])

HSP 1 Score: 271.9 bits (694), Expect = 3.2e-69
Identity = 128/148 (86.49%), Postives = 137/148 (92.57%), Query Frame = 0

Query: 1   MARNTSISIPKHLSSAILLLLLLLAVVSDCSNNESRVECSRTCVAINCNTVGIRYGKFCG 60
           M R+ SI I KH SS  LL+LL L+VVS+CS+NESRVECSRTCVAINCNTVGIRYGKFCG
Sbjct: 1   MVRHPSIPIRKHFSSPFLLVLLFLSVVSECSSNESRVECSRTCVAINCNTVGIRYGKFCG 60

Query: 61  VGWTGCAGEKPCDDLDACCKVHDECVERKGLTNIKCHEKFKTCIKRVQKSGKVGFSQECP 120
           VGWTGCAGEKPCDDLDACCKVHDECVERKGLTNIKCHEKFK+CIK+VQKSGK GFS+ECP
Sbjct: 61  VGWTGCAGEKPCDDLDACCKVHDECVERKGLTNIKCHEKFKSCIKKVQKSGKAGFSEECP 120

Query: 121 YSTAVPTMVQGMDLAIMFSQFGNSKLEL 149
           YSTAVP MVQGMDLAI+FSQ GNSKLEL
Sbjct: 121 YSTAVPIMVQGMDLAIVFSQLGNSKLEL 148

BLAST of Lsi11G001980 vs. NCBI nr
Match: XP_004141047.1 (probable phospholipase A2 homolog 1 [Cucumis sativus] >XP_031737108.1 probable phospholipase A2 homolog 1 [Cucumis sativus] >KGN60548.1 hypothetical protein Csa_019342 [Cucumis sativus])

HSP 1 Score: 268.5 bits (685), Expect = 3.5e-68
Identity = 130/148 (87.84%), Postives = 136/148 (91.89%), Query Frame = 0

Query: 1   MARNTSISIPKHLSSAILLLLLLLAVVSDCSNNESRVECSRTCVAINCNTVGIRYGKFCG 60
           MARNT IS        ILLLLLLL VVS CSNNESRVECSRTCVAINCNTVGIRYGKFCG
Sbjct: 1   MARNTIIS--------ILLLLLLLTVVSHCSNNESRVECSRTCVAINCNTVGIRYGKFCG 60

Query: 61  VGWTGCAGEKPCDDLDACCKVHDECVERKGLTNIKCHEKFKTCIKRVQKSGKVGFSQECP 120
           VGWTGCAGEKPCDDLDACCKVHDECVERKGLTN+KCHEKFK+CIK+VQKSGKVGFS +CP
Sbjct: 61  VGWTGCAGEKPCDDLDACCKVHDECVERKGLTNVKCHEKFKSCIKKVQKSGKVGFSNDCP 120

Query: 121 YSTAVPTMVQGMDLAIMFSQFGNSKLEL 149
           YSTAVPTMVQGM+LAIMFS+FGNSKLEL
Sbjct: 121 YSTAVPTMVQGMNLAIMFSKFGNSKLEL 140

BLAST of Lsi11G001980 vs. NCBI nr
Match: XP_022999541.1 (probable phospholipase A2 homolog 1 [Cucurbita maxima])

HSP 1 Score: 237.3 bits (604), Expect = 8.6e-59
Identity = 113/148 (76.35%), Postives = 127/148 (85.81%), Query Frame = 0

Query: 1   MARNTSISIPKHLSSAILLLLLLLAVVSDCSNNESRVECSRTCVAINCNTVGIRYGKFCG 60
           M  NTSIS+ KH++SAI LLL+LL+VVS+CS   S+VECSRTCV+  CNTVGIRYGKFCG
Sbjct: 1   MVCNTSISLRKHVASAIFLLLVLLSVVSECS---SQVECSRTCVSEKCNTVGIRYGKFCG 60

Query: 61  VGWTGCAGEKPCDDLDACCKVHDECVERKGLTNIKCHEKFKTCIKRVQKSGKVGFSQECP 120
           VGWTGCAGEKPCDDLDACCK HDECVE KGLTNIKCH KFK CI ++QKSG  GFSQ+CP
Sbjct: 61  VGWTGCAGEKPCDDLDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCP 120

Query: 121 YSTAVPTMVQGMDLAIMFSQFGNSKLEL 149
           YS A+PTMV GMD+AIMFSQF + K EL
Sbjct: 121 YSIAIPTMVNGMDIAIMFSQFSSGKFEL 145

BLAST of Lsi11G001980 vs. TAIR 10
Match: AT2G19690.1 (phospholipase A2-beta )

HSP 1 Score: 164.9 bits (416), Expect = 5.1e-41
Identity = 71/118 (60.17%), Postives = 91/118 (77.12%), Query Frame = 0

Query: 38  ECSRTCVAINCNTVGIRYGKFCGVGWTGCAGEKPCDDLDACCKVHDECVERKGLTNIKCH 97
           EC+RTC+A NC+T+ IRYGK+CG+G +GC GE+PCDDLDACCK+HD CVE  G+TNI CH
Sbjct: 30  ECTRTCIAQNCDTLSIRYGKYCGIGHSGCPGEEPCDDLDACCKIHDHCVELNGMTNISCH 89

Query: 98  EKFKTCIKRV------QKSGKVGFSQECPYSTAVPTMVQGMDLAIMFSQFGNS-KLEL 149
           +KF+ C+ R+       K+ KVGFS +CPYS  +PT+ QGMD+ I+FSQ GN  K EL
Sbjct: 90  KKFQRCVNRLSKAIKQSKNKKVGFSTKCPYSVVIPTVNQGMDIGILFSQLGNDMKTEL 147

BLAST of Lsi11G001980 vs. TAIR 10
Match: AT2G19690.2 (phospholipase A2-beta )

HSP 1 Score: 159.5 bits (402), Expect = 2.1e-39
Identity = 66/109 (60.55%), Postives = 86/109 (78.90%), Query Frame = 0

Query: 38  ECSRTCVAINCNTVGIRYGKFCGVGWTGCAGEKPCDDLDACCKVHDECVERKGLTNIKCH 97
           EC+RTC+A NC+T+ IRYGK+CG+G +GC GE+PCDDLDACCK+HD CVE  G+TNI CH
Sbjct: 30  ECTRTCIAQNCDTLSIRYGKYCGIGHSGCPGEEPCDDLDACCKIHDHCVELNGMTNISCH 89

Query: 98  EKFKTCIKRV------QKSGKVGFSQECPYSTAVPTMVQGMDLAIMFSQ 141
           +KF+ C+ R+       K+ KVGFS +CPYS  +PT+ QGMD+ I+FSQ
Sbjct: 90  KKFQRCVNRLSKAIKQSKNKKVGFSTKCPYSVVIPTVNQGMDIGILFSQ 138

BLAST of Lsi11G001980 vs. TAIR 10
Match: AT4G29460.1 (Phospholipase A2 family protein )

HSP 1 Score: 155.6 bits (392), Expect = 3.1e-38
Identity = 71/132 (53.79%), Postives = 91/132 (68.94%), Query Frame = 0

Query: 18  LLLLLLLAVVSDCSNNESRVECSRTCVAINCNTVGIRYGKFCGVGWTGCAGEKPCDDLDA 77
           L   LLLAVVS      S+ +CS TC+A NCN++GIRYGK+CG+G+ GC GE PCDDLDA
Sbjct: 14  LTAFLLLAVVS------SQEKCSNTCIAQNCNSLGIRYGKYCGIGYFGCPGEPPCDDLDA 73

Query: 78  CCKVHDECVERKGLTNIKCHEKFKTCIKRVQKS------GKVGFSQECPYSTAVPTMVQG 137
           CC  HD CV+ KG+T + CH++FK C+ ++ KS       K+GFS +CPYS  +PT+  G
Sbjct: 74  CCMTHDNCVDLKGMTYVNCHKQFKRCVNKLSKSIKHSNGEKIGFSTQCPYSIVIPTVFNG 133

Query: 138 MDLAIMFSQFGN 144
           MD  I FS  GN
Sbjct: 134 MDYGIFFSGIGN 139

BLAST of Lsi11G001980 vs. TAIR 10
Match: AT4G29470.1 (Phospholipase A2 family protein )

HSP 1 Score: 150.2 bits (378), Expect = 1.3e-36
Identity = 68/132 (51.52%), Postives = 92/132 (69.70%), Query Frame = 0

Query: 18  LLLLLLLAVVSDCSNNESRVECSRTCVAINCNTVGIRYGKFCGVGWTGCAGEKPCDDLDA 77
           L + LLLAVV       S+ +CS+TC+A  CN +GIRYGK+CG+G+ GC GE PCDDLD 
Sbjct: 14  LTVFLLLAVV------HSQEKCSKTCIAQKCNVLGIRYGKYCGIGYFGCPGEPPCDDLDD 73

Query: 78  CCKVHDECVERKGLTNIKCHEKFKTCIKRVQKS------GKVGFSQECPYSTAVPTMVQG 137
           CC  HD CV+ KG+T + CH++F+ C+  +++S       KVGFS+ECPYST +PT+ +G
Sbjct: 74  CCMTHDNCVDLKGMTYVDCHKQFQRCVNELKQSIQESNNQKVGFSKECPYSTVIPTVYRG 133

Query: 138 MDLAIMFSQFGN 144
           M+  I FS  GN
Sbjct: 134 MNYGIFFSGIGN 139

BLAST of Lsi11G001980 vs. TAIR 10
Match: AT2G06925.1 (Phospholipase A2 family protein )

HSP 1 Score: 73.9 bits (180), Expect = 1.2e-13
Identity = 36/104 (34.62%), Postives = 58/104 (55.77%), Query Frame = 0

Query: 38  ECSRTCVAINCNTVG-IRYGKFCGVGWTGCAGEKPCDDLDACCKVHDECVERKG--LTNI 97
           ECSR C +  C+    +RYGK+CG+ ++GC GE+PCD LD+CC  HD CV+ K     + 
Sbjct: 37  ECSRKCESEFCSVPPFLRYGKYCGLLYSGCPGERPCDGLDSCCMKHDACVQSKNNDYLSQ 96

Query: 98  KCHEKFKTCIKRVQKSGKVGF-SQECPYSTAVPTMVQGMDLAIM 138
           +C +KF  C+    +  +  F   +C     +  +   M+ A++
Sbjct: 97  ECSQKFINCMNNFSQKKQPTFKGNKCDADEVIDVISIVMEAALI 140

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9XG808.7e-4658.96Probable phospholipase A2 homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q8GZB47.2e-4060.17Phospholipase A2-beta OS=Arabidopsis thaliana OX=3702 GN=PLA2-BETA PE=1 SV=1[more]
Q9M0D74.3e-3753.79Phospholipase A2-gamma OS=Arabidopsis thaliana OX=3702 GN=PLA2-GAMMA PE=1 SV=1[more]
Q8GV501.8e-3551.52Phospholipase A2-delta OS=Arabidopsis thaliana OX=3702 GN=PLA2-DELTA PE=2 SV=1[more]
Q10E504.7e-1535.64Phospholipase A2 homolog 3 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-III ... [more]
Match NameE-valueIdentityDescription
A0A1S3C9861.1e-7089.86probable phospholipase A2 homolog 1 OS=Cucumis melo OX=3656 GN=LOC103498425 PE=4... [more]
A0A6J1DMI51.5e-6986.49probable phospholipase A2 homolog 1 OS=Momordica charantia OX=3673 GN=LOC1110219... [more]
A0A0A0LFR91.7e-6887.84Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000560 PE=4 SV=1[more]
A0A6J1KDD24.2e-5976.35probable phospholipase A2 homolog 1 OS=Cucurbita maxima OX=3661 GN=LOC111493870 ... [more]
W9QS246.0e-5873.24Putative phospholipase A2-1-like protein OS=Morus notabilis OX=981085 GN=L484_02... [more]
Match NameE-valueIdentityDescription
XP_038889610.13.5e-7695.95probable phospholipase A2 homolog 1 [Benincasa hispida][more]
XP_008459246.12.2e-7089.86PREDICTED: probable phospholipase A2 homolog 1 [Cucumis melo][more]
XP_022154742.13.2e-6986.49probable phospholipase A2 homolog 1 [Momordica charantia] >XP_022154743.1 probab... [more]
XP_004141047.13.5e-6887.84probable phospholipase A2 homolog 1 [Cucumis sativus] >XP_031737108.1 probable p... [more]
XP_022999541.18.6e-5976.35probable phospholipase A2 homolog 1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G19690.15.1e-4160.17phospholipase A2-beta [more]
AT2G19690.22.1e-3960.55phospholipase A2-beta [more]
AT4G29460.13.1e-3853.79Phospholipase A2 family protein [more]
AT4G29470.11.3e-3651.52Phospholipase A2 family protein [more]
AT2G06925.11.2e-1334.62Phospholipase A2 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036444Phospholipase A2 domain superfamilyGENE3D1.20.90.10Phospholipase A2 domaincoord: 25..145
e-value: 1.2E-31
score: 111.0
IPR036444Phospholipase A2 domain superfamilySUPERFAMILY48619Phospholipase A2, PLA2coord: 37..144
NoneNo IPR availablePANTHERPTHR11716:SF92BNAA09G09940D PROTEINcoord: 21..144
IPR001211Phospholipase A2PANTHERPTHR11716PHOSPHOLIPASE A2 FAMILY MEMBERcoord: 21..144
IPR033113Phospholipase A2, histidine active sitePROSITEPS00118PA2_HIScoord: 78..85

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi11G001980.1Lsi11G001980.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050482 arachidonic acid secretion
biological_process GO:0016042 lipid catabolic process
biological_process GO:0006644 phospholipid metabolic process
biological_process GO:0009555 pollen development
biological_process GO:0009846 pollen germination
biological_process GO:0009860 pollen tube growth
cellular_component GO:0005576 extracellular region
molecular_function GO:0047498 calcium-dependent phospholipase A2 activity
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0005543 phospholipid binding
molecular_function GO:0004623 phospholipase A2 activity