Lsi11G000340 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi11G000340
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionP-loop containing nucleoside triphosphate hydrolases superfamily protein
Locationchr11: 352634 .. 356349 (-)
RNA-Seq ExpressionLsi11G000340
SyntenyLsi11G000340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTGGTGTTTCTTCCATCAATCATCCGGATCTACGCCGGTACTATCCAGGTGCGAAGAACCCCGGGGGTGACTGCAATGAGCAGAAATCTCACTCACCGGTCCGCTACTCCTATTAGCGCCGGACCTTGAGTCGAATTGATCGTGTGTCATGTGCAACGTCCATCAATGATGGTTCATTAATGTCTTGTGAGATTTGCTTTTGATTTGGTCCCTAAATTTTAAGATTTACATTTTTAACCCTAACTTATCATTAGATACTTTCAATTTTAGCGTTAATGTCTATTAAGTTAATTTAAAATATTAATTATGAAGTGAAATTTTAAAATTAATTTTAATAATGATGGAAAAGAACAGTAAAATTTAATTAATTATAATCATTTTAATTATTTTAAATTAATTATTAAAGACTGAAAGTAAGTATTTAGCAAAAAAAAAAATTGAGGTTAAAAGTATAAATCTTGAAACCTAGAGAGCAAATCGAAACAAACTTCAAAGCTCATTGGTAAAATGGTAACACTTTAAAATTTATAGAAACTAAATTGAAATCAAACTCAAAACTAAAACACTAAATATATAACATTTTGAAACCTAAAAACTATATAAAAATTAAATTCAAAAGCTGGAGACTAAAAATAAAAGAAATTACAAAAAGCAAAAGCATATAATGTGTACGTTGTAGAAAAAAGTTTTTTAAAAAAAAACATTTAATTACATAGAGGTGATTGTGTTTGTGAATGGTGAGTTATGAGAGTAGAGGAAACGAGGAGTTGCCCAAAGGCCATCTCTTGCAGTTCCCAAGAATAAAACAATAATAACAATAATAATAGCCAACTCTTTAATTTGTTTTAAATTTGAAGTTTGTCCATCCAATTTTCTCATCTCGATCTTCTTAATGATCTTTTCCATTTCAGACATCATAGTTCATAAGAAAGGGTGTGATTGATTAATTATGGAAGAAGCTGATCATCATGAGGAAAGTGGAAGCAGTAGTTTTGTGGCAAAGTTTGAAGCTTTGGATATCACTACTAGAAATAATAAAGCAATAATTAGCAAAAGATCAATGTACAAGAATTACAACAACTCATGTCTTCCTAAAAGAAGAATGTCCAATGAAAGTGGTCTTATTCTCCTTATCAAATCTTGGTCCCTTGAAGATGTTTTCAATCAACACCTATACAAATCCAAGGTTCTTTCTCTAATTTCCCTTCTCTTTTTTGTTATGCCATGCACATATATTATATATATTAGTAAATAGTATGATTTTTTCTTCAAATAAGTTTCTAACATTATCATTTTGGTTTTTGAATTTTCAAAGCTTTTTCATCTCATTTTAATTCTTTAACTCTAGTTGAAAAGTATGTAGATTTGTTTCATTTTGGTATAAGTAACTTTTGAAATGTCTACTTTAGAGCTCGTTTGGTAATGATTTTGTATTTAAAAAATTGTTTGGTAACATTATCGTCTTGGTTTTTTAATCTAAGTTTCATATACTATGGTTTTCACATTAACAAAGTGAAGTAACTATTTAGTTTTTAATCATATTAAGAAAAAAACAAAATTACTTTTTTTAGACTTTGTTTCTAATTATTTAGTTTTTTGTTTTGGTTTTTTAGAATTAAGCTTATTTCCTTTTAGTTTCTTATCATGATTTGCATCTCTCTATTGAGTTTTTAGTCAATTTCCAAAACCAAAACACAAATTTTTAAAAACTATTTTTTTTTAAAAAAATTAGCTTGGTTTTAAAAAATAGTAGTGTAAAAAGTAGATAATTAGCGAAACAAGAAATTTAGAAGTGAAATAGGTGTTTATAAGCTTGAATTTTCAAAAATTAAAAATAAAAAAAAATCAAATTGTTACCAAACGGGCTTTAGTTTTCAAAATTTTGCTTAGTCTTTTAAAACATACTTATAAAGTGTGTAACAAAAAAAACTTATAGGTAAAAAGAAAGTAGTGTTTATAAACTTAACTTTCAATAAAATTAAATAATTATCAAACAATGCCTTTAATGATTTTGAAATTATAACGATTTTCCTTTTTTTACAGATAAAAAGGATTCCATTATGTTTTGATTCATTAGATCAATGTTTCATTGTCGTTGTCGTCGAGCATGGATCCCATTTCCAAAGCTCCATTCACTCAAGTAATCTCCTTGGAAGAGATAAACCCAAGTGAGTTAGGATTATACAACATCCAAATCAACAAATGGCAAAATGGTCACAAGGAATCCTCAACTCTAGGAGACATATTCATCTTGTCGGATGTCAAACCGAATCTCGTTTCCGATTTGCGAAGAATCGGAAAAACATGGACTTTTGCATCCTTTTTCCCAAACAAAAACGACGACGACAACGACAATGAAACATTCATGTCTTCTTTTATCTTAAAAACATGGCAACCCAATTTCAAAATGAACTCAAAACAACCCATCTTCCTAATCTATTTGGTCAATATACTTCCCTATATTAGAATATGGAATGCCCTCCACATGAACAACAAAAACAACTCATCCATTTTCAACCAAATTCTTAGCTCCAACAATAACAATAATAATAGTTCATCGAATCGTCATTGCAATGAGCGTGATGAATTATTATCCGACACGAGCAGTTTGTTCTCGATGTTGAATGAATCCCAAGTTAAAGCCATTGGGTCTTGTTTGAAAACCATAAGCTGTGAGGACAAATGTGGGATTGAACTCATATGGGGTCCACCAGGAACAGGCAAAACAATCACGGTTGGAATTTTGTTATTCCAATTGTTGAGGAATCAATGTCGGACAATTGCTTGTGCTCCAACCAACACTGCAATTATGCAATTGGCTTCCAAGTTTCTTGTTTTGGTAAAAGAAATGCATGAGAAAAAATGTGGATCTGAAGGGATTTTTTGTTGTTTAGGTGACATTCTTTTGTTTGGGAATAAGTCAAAACTCAAAGTTGAGTTTACCGATAAATATATGTTTTTCGATTATCGAGTCGAAAGGCTTAAAAAATGCTTCAATCCCTTTACTGGTTGGAGACATTGCTTTGCATCCATGATTGATTTTCTCGAAGATTGTGTTCTCCAATTTTAGGATGTCGACGACTACATGTCCTTTCTCGAGTTCGTACAAACAAGGTTTAGAGTCATCGCATCGTCGCTCCGAGATTGTGTGTCGATCTTTTGCACTCATGTTTCGAGGAGTGTTTTGAAGTGTAATTTTGAGAGAATGGGTTGTTGTCTCATGAGTTTAATTGATTCTTTGCAAAGTTTGTTGTTTCAAAATTGGGTTGCATCTGAGGAGCTTAAGAGGGTATTTTCATCCAATCAAAATGATGCCTATGGATCATTATTTACCAAATATGAGGAGCTTTTGAAGACAAGAAATGATTGCATTGTAGTTTTGAAATCTCTTCAACAATCCTTGGATTTGCTTGGTCTTCCACAAACAACAAATAAAAGAACCATTGTGAATTTCTGTTTTCAGAATGCTTCATTGTTCTTTTGCACGGTTTCGAGTTTGTTTAAGTTGTATTCGAAGTCATTGGAGCCATTGAAGGCTTTGGTGATTGATGAAGCAGCACAGTTGAAAGAGTGTGAGTCTGTTATTCCAATGCAACTTCCTGATATAATGCATGCAATTCTCATAGGTGATGAGTGCCAATTGCCAGCCATGGTTGAGAGCAAAGTAAGAAAATTTCTTCCCTTTTCAATCATATGTGATAGATACGATTAG

mRNA sequence

ATGTTTGGTGTTTCTTCCATCAATCATCCGGATCTACGCCGGTACTATCCAGGTGCGAAGAACCCCGGGGGTGACTGCAATGAGCAGAAATCTCACTCACCGATCAATGTTTCATTGTCGTTGTCGTCGAGCATGGATCCCATTTCCAAAGCTCCATTCACTCAAGTAATCTCCTTGGAAGAGATAAACCCAAGTGAGTTAGGATTATACAACATCCAAATCAACAAATGGCAAAATGGTCACAAGGAATCCTCAACTCTAGGAGACATATTCATCTTGTCGGATGTCAAACCGAATCTCGTTTCCGATTTGCGAAGAATCGGAAAAACATGGACTTTTGCATCCTTTTTCCCAAACAAAAACGACGACGACAACGACAATGAAACATTCATGTCTTCTTTTATCTTAAAAACATGGCAACCCAATTTCAAAATGAACTCAAAACAACCCATCTTCCTAATCTATTTGGTCAATATACTTCCCTATATTAGAATATGGAATGCCCTCCACATGAACAACAAAAACAACTCATCCATTTTCAACCAAATTCTTAGCTCCAACAATAACAATAATAATAGTTCATCGAATCGTCATTGCAATGAGCGTGATGAATTATTATCCGACACGAGCAGTTTGTTCTCGATGTTGAATGAATCCCAAGTTAAAGCCATTGGGTCTTGTTTGAAAACCATAAGCTGTGAGGACAAATGTGGGATTGAACTCATATGGGGTCCACCAGGAACAGGCAAAACAATCACGGTTGGAATTTTGTTATTCCAATTGTTGAGGAATCAATGTCGGACAATTGCTTGTGCTCCAACCAACACTGCAATTATGCAATTGGCTTCCAAGTTTCTTGTTTTGGATGTCGACGACTACATGTCCTTTCTCGAGTTCGTACAAACAAGGTTTAGAGTCATCGCATCGTCGCTCCGAGATTGTGTGTCGATCTTTTGCACTCATGTTTCGAGGAGTGTTTTGAAGTGTAATTTTGAGAGAATGGGTTGTTGTCTCATGAGTTTAATTGATTCTTTGCAAAGTTTGTTGTTTCAAAATTGGGTTGCATCTGAGGAGCTTAAGAGGGTATTTTCATCCAATCAAAATGATGCCTATGGATCATTATTTACCAAATATGAGGAGCTTTTGAAGACAAGAAATGATTGCATTGTAGTTTTGAAATCTCTTCAACAATCCTTGGATTTGCTTGGTCTTCCACAAACAACAAATAAAAGAACCATTGTGAATTTCTGTTTTCAGAATGCTTCATTGTTCTTTTGCACGGTTTCGAGTTTGTTTAAGTTGTATTCGAAGTCATTGGAGCCATTGAAGGCTTTGGTGATTGATGAAGCAGCACAGTTGAAAGAGTGTGAGTCTGTTATTCCAATGCAACTTCCTGATATAATGCATGCAATTCTCATAGGTGATGAGTGCCAATTGCCAGCCATGGTTGAGAGCAAAGTAAGAAAATTTCTTCCCTTTTCAATCATATGTGATAGATACGATTAG

Coding sequence (CDS)

ATGTTTGGTGTTTCTTCCATCAATCATCCGGATCTACGCCGGTACTATCCAGGTGCGAAGAACCCCGGGGGTGACTGCAATGAGCAGAAATCTCACTCACCGATCAATGTTTCATTGTCGTTGTCGTCGAGCATGGATCCCATTTCCAAAGCTCCATTCACTCAAGTAATCTCCTTGGAAGAGATAAACCCAAGTGAGTTAGGATTATACAACATCCAAATCAACAAATGGCAAAATGGTCACAAGGAATCCTCAACTCTAGGAGACATATTCATCTTGTCGGATGTCAAACCGAATCTCGTTTCCGATTTGCGAAGAATCGGAAAAACATGGACTTTTGCATCCTTTTTCCCAAACAAAAACGACGACGACAACGACAATGAAACATTCATGTCTTCTTTTATCTTAAAAACATGGCAACCCAATTTCAAAATGAACTCAAAACAACCCATCTTCCTAATCTATTTGGTCAATATACTTCCCTATATTAGAATATGGAATGCCCTCCACATGAACAACAAAAACAACTCATCCATTTTCAACCAAATTCTTAGCTCCAACAATAACAATAATAATAGTTCATCGAATCGTCATTGCAATGAGCGTGATGAATTATTATCCGACACGAGCAGTTTGTTCTCGATGTTGAATGAATCCCAAGTTAAAGCCATTGGGTCTTGTTTGAAAACCATAAGCTGTGAGGACAAATGTGGGATTGAACTCATATGGGGTCCACCAGGAACAGGCAAAACAATCACGGTTGGAATTTTGTTATTCCAATTGTTGAGGAATCAATGTCGGACAATTGCTTGTGCTCCAACCAACACTGCAATTATGCAATTGGCTTCCAAGTTTCTTGTTTTGGATGTCGACGACTACATGTCCTTTCTCGAGTTCGTACAAACAAGGTTTAGAGTCATCGCATCGTCGCTCCGAGATTGTGTGTCGATCTTTTGCACTCATGTTTCGAGGAGTGTTTTGAAGTGTAATTTTGAGAGAATGGGTTGTTGTCTCATGAGTTTAATTGATTCTTTGCAAAGTTTGTTGTTTCAAAATTGGGTTGCATCTGAGGAGCTTAAGAGGGTATTTTCATCCAATCAAAATGATGCCTATGGATCATTATTTACCAAATATGAGGAGCTTTTGAAGACAAGAAATGATTGCATTGTAGTTTTGAAATCTCTTCAACAATCCTTGGATTTGCTTGGTCTTCCACAAACAACAAATAAAAGAACCATTGTGAATTTCTGTTTTCAGAATGCTTCATTGTTCTTTTGCACGGTTTCGAGTTTGTTTAAGTTGTATTCGAAGTCATTGGAGCCATTGAAGGCTTTGGTGATTGATGAAGCAGCACAGTTGAAAGAGTGTGAGTCTGTTATTCCAATGCAACTTCCTGATATAATGCATGCAATTCTCATAGGTGATGAGTGCCAATTGCCAGCCATGGTTGAGAGCAAAGTAAGAAAATTTCTTCCCTTTTCAATCATATGTGATAGATACGATTAG

Protein sequence

MFGVSSINHPDLRRYYPGAKNPGGDCNEQKSHSPINVSLSLSSSMDPISKAPFTQVISLEEINPSELGLYNIQINKWQNGHKESSTLGDIFILSDVKPNLVSDLRRIGKTWTFASFFPNKNDDDNDNETFMSSFILKTWQPNFKMNSKQPIFLIYLVNILPYIRIWNALHMNNKNNSSIFNQILSSNNNNNNSSSNRHCNERDELLSDTSSLFSMLNESQVKAIGSCLKTISCEDKCGIELIWGPPGTGKTITVGILLFQLLRNQCRTIACAPTNTAIMQLASKFLVLDVDDYMSFLEFVQTRFRVIASSLRDCVSIFCTHVSRSVLKCNFERMGCCLMSLIDSLQSLLFQNWVASEELKRVFSSNQNDAYGSLFTKYEELLKTRNDCIVVLKSLQQSLDLLGLPQTTNKRTIVNFCFQNASLFFCTVSSLFKLYSKSLEPLKALVIDEAAQLKECESVIPMQLPDIMHAILIGDECQLPAMVESKVRKFLPFSIICDRYD
Homology
BLAST of Lsi11G000340 vs. ExPASy Swiss-Prot
Match: Q9FJR0 (Regulator of nonsense transcripts 1 homolog OS=Arabidopsis thaliana OX=3702 GN=UPF1 PE=1 SV=2)

HSP 1 Score: 54.3 bits (129), Expect = 4.6e-06
Identity = 30/70 (42.86%), Postives = 42/70 (60.00%), Query Frame = 0

Query: 216 LNESQVKAIGSCLKTISCEDKCGIELIWGPPGTGKTITVGILLFQLLR-NQCRTIACAPT 275
           LN SQV A+ S L+         I LI GPPGTGKT+T   +++ + +  Q + + CAP+
Sbjct: 489 LNASQVNAVKSVLQK-------PISLIQGPPGTGKTVTSAAIVYHMAKQGQGQVLVCAPS 548

Query: 276 NTAIMQLASK 285
           N A+ QLA K
Sbjct: 549 NVAVDQLAEK 551

BLAST of Lsi11G000340 vs. ExPASy Swiss-Prot
Match: P30771 (ATP-dependent helicase NAM7 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=NAM7 PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 3.0e-05
Identity = 28/73 (38.36%), Postives = 43/73 (58.90%), Query Frame = 0

Query: 213 FSMLNESQVKAIGSCLKTISCEDKCGIELIWGPPGTGKTITVGILLFQLLR-NQCRTIAC 272
           F+ LN SQ  A+   L+         + LI GPPGTGKT+T   +++ L + ++ R + C
Sbjct: 406 FAQLNSSQSNAVSHVLQR-------PLSLIQGPPGTGKTVTSATIVYHLSKIHKDRILVC 465

Query: 273 APTNTAIMQLASK 285
           AP+N A+  LA+K
Sbjct: 466 APSNVAVDHLAAK 471

BLAST of Lsi11G000340 vs. ExPASy Swiss-Prot
Match: Q9HEH1 (Regulator of nonsense transcripts 1 homolog OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=2E4.130 PE=3 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 1.5e-04
Identity = 26/70 (37.14%), Postives = 39/70 (55.71%), Query Frame = 0

Query: 216 LNESQVKAIGSCLKTISCEDKCGIELIWGPPGTGKTITVGILLFQLLR-NQCRTIACAPT 275
           LN SQ+ AI   L          + LI GPPGTGKT+T   +++ L + +  + + CAP+
Sbjct: 456 LNASQIAAIKQVLSN-------PLSLIQGPPGTGKTVTSATIIYHLAKMSNSQVLVCAPS 515

Query: 276 NTAIMQLASK 285
           N A+ QL  +
Sbjct: 516 NVAVDQLCER 518

BLAST of Lsi11G000340 vs. ExPASy Swiss-Prot
Match: Q92900 (Regulator of nonsense transcripts 1 OS=Homo sapiens OX=9606 GN=UPF1 PE=1 SV=2)

HSP 1 Score: 48.9 bits (115), Expect = 1.9e-04
Identity = 28/70 (40.00%), Postives = 39/70 (55.71%), Query Frame = 0

Query: 216 LNESQVKAIGSCLKTISCEDKCGIELIWGPPGTGKTITVGILLFQLLR-NQCRTIACAPT 275
           LN SQV A+ + L+         + LI GPPGTGKT+T   +++ L R      + CAP+
Sbjct: 482 LNHSQVYAVKTVLQR-------PLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPS 541

Query: 276 NTAIMQLASK 285
           N A+ QL  K
Sbjct: 542 NIAVDQLTEK 544

BLAST of Lsi11G000340 vs. ExPASy Swiss-Prot
Match: Q9EPU0 (Regulator of nonsense transcripts 1 OS=Mus musculus OX=10090 GN=Upf1 PE=1 SV=2)

HSP 1 Score: 48.9 bits (115), Expect = 1.9e-04
Identity = 28/70 (40.00%), Postives = 39/70 (55.71%), Query Frame = 0

Query: 216 LNESQVKAIGSCLKTISCEDKCGIELIWGPPGTGKTITVGILLFQLLR-NQCRTIACAPT 275
           LN SQV A+ + L+         + LI GPPGTGKT+T   +++ L R      + CAP+
Sbjct: 477 LNHSQVYAVKTVLQR-------PLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPS 536

Query: 276 NTAIMQLASK 285
           N A+ QL  K
Sbjct: 537 NIAVDQLTEK 539

BLAST of Lsi11G000340 vs. ExPASy TrEMBL
Match: A0A1S3CEY4 (uncharacterized protein LOC103500100 OS=Cucumis melo OX=3656 GN=LOC103500100 PE=4 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 4.2e-111
Identity = 248/540 (45.93%), Postives = 331/540 (61.30%), Query Frame = 0

Query: 41  LSSSMDPISKAPFTQVISLEEINPSELGLYNIQINKWQNGHKESSTLGDIFILSDVKPNL 100
           L SSM+PISK+P  QVI+L+EI     GL+ I +   +         G+IFILS+VKP +
Sbjct: 52  LYSSMNPISKSPCVQVIALKEIKTYGKGLFEIHLKDCKQYCPTILIPGNIFILSNVKPKV 111

Query: 101 VSDLRRIGKTWTFASFFPNKNDDDNDNETFMSSFILKTWQPNFKM---NSKQPIFLIYLV 160
           VSDL+R G+TWTFA+F   K    + N+   + F +K W+ NF     + + P+FL+ LV
Sbjct: 112 VSDLQRNGRTWTFATFVSKKGKKKDKNKP--TCFTIKIWKENFMKDLNHHEHPMFLVVLV 171

Query: 161 NILPYIRIWNALHMNNKNNSSIFNQILSSNNNNNNSSSNRH-CNERDELLSDTSSLFSML 220
           N+L  IRIWNALHM  K N++IFN +L  N++N+N +     C  + + LS  +SLF  L
Sbjct: 172 NVLTNIRIWNALHM--KKNNAIFNHVLGVNSSNSNLNFGCDVCETKIDELSSNNSLFFTL 231

Query: 221 NESQVKAIGSCLKTISCEDKCGIELIWGPPGTGKTITVGILLFQLLRNQCRTIACAPTNT 280
           NESQ +A+G+CLK  SC  K G+ELIWGPPGTGKT TVG+LLF+L +   RT+ACAPTNT
Sbjct: 232 NESQARAVGTCLKRTSCAHKYGVELIWGPPGTGKTKTVGVLLFELRKKNRRTLACAPTNT 291

Query: 281 AIMQLASKFLVL------------------------------------DVDDYM------ 340
           AIMQ+AS+FL+L                                    D D Y+      
Sbjct: 292 AIMQVASRFLLLVKEMHGKKDNRSKKLFCNLGDILLFGNKERLKVGESDKDIYLDYRIGR 351

Query: 341 ---------------------------------------------SFLEFVQTRFRVIAS 400
                                                        SF+EFV+  ++ I+ 
Sbjct: 352 LEKCFNQFNGWRVCFSSMIDFLEGYCVSQYRAFLKDKQRPKKFEYSFIEFVRVYYKTISC 411

Query: 401 SLRDCVSIFCTHVSRSVLKCNFERMGCCLMSLIDSLQSLLFQNWVASEELKRVFSSN-QN 460
           SL++C+SIFCTH+  ++LK NFER+ CC+MSLI+S +SLL  N V S+EL+++FS   + 
Sbjct: 412 SLKECISIFCTHIPIAILKHNFERL-CCVMSLIESFESLLLSNGVESKELEKLFSKKVEE 471

Query: 461 DAYGSLFTKYEELLKTRNDCIVVLKSLQQSLDLLGLPQTTNKRTIVNFCFQNASLFFCTV 488
           +   +   +YE+LLK RNDC++VL+SL+ SL  L LPQT+ +R +  FCF+NASLFFCTV
Sbjct: 472 EVVVNQNVEYEKLLKGRNDCVLVLRSLKYSLGELKLPQTSRERRLRAFCFRNASLFFCTV 531

BLAST of Lsi11G000340 vs. ExPASy TrEMBL
Match: A0A0A0K415 (AAA_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G291110 PE=4 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 5.1e-109
Identity = 248/544 (45.59%), Postives = 327/544 (60.11%), Query Frame = 0

Query: 41  LSSSMDPISKAPFTQVISLEEINPSELGLYNIQINKWQNGHKESSTLGDIFILSDVKPNL 100
           L SSM+PISK+P  QVISL+EI P   GL+ I +   +         G+IFILS+VKP +
Sbjct: 87  LHSSMNPISKSPCVQVISLKEIKPYGKGLFQIHLKDCKQYCPTILIPGNIFILSNVKPKV 146

Query: 101 VSDLRRIGKTWTFA-SFFPNKNDDDNDNETFMSSFILKTWQPNFKM---NSKQPIFLIYL 160
           VSDL+  GKTWTFA +F   K      N+   + F +KTW+ NF     + + P FL+ L
Sbjct: 147 VSDLQGNGKTWTFATTFVSQKGKKKGTNKP--TCFTIKTWKENFMKDLNHHEHPTFLVVL 206

Query: 161 VNILPYIRIWNALHMNNKNNSSIFNQILSSNNNNNNSSSNRH-CNERDELLSDTSSLFSM 220
           VN+L  +RIWNALHM  K N++IFN +L   + N+N +     C  + E LS  SSLF  
Sbjct: 207 VNVLTNVRIWNALHM--KKNNAIFNHVLGVTSFNSNLNFGCDVCETKIEELSSKSSLFCT 266

Query: 221 LNESQVKAIGSCLKTISCEDKCGIELIWGPPGTGKTITVGILLFQLLRNQCRTIACAPTN 280
           LNESQ +A+G+CL  ISC  K G+ELIWGPPGTGKT TVG+LLF+L +   RT+ACAPTN
Sbjct: 267 LNESQARAVGTCLNRISCVHKYGVELIWGPPGTGKTKTVGVLLFELRKKNRRTLACAPTN 326

Query: 281 TAIMQLASKFLVL----------------------------------DVDDYM------- 340
           TAIMQ+AS+FL+L                                  + D Y+       
Sbjct: 327 TAIMQVASRFLLLVKEMHDKKDNGSKGLFCNLGDILLFGNKERLKVGESDKYIYLDYRIG 386

Query: 341 -----------------------------------------------SFLEFVQTRFRVI 400
                                                          SF+EFV+  ++ I
Sbjct: 387 RLKKCFNQLNGWRFCFSSMIDFLEGHCVSQYRTFLKDHKQRSKMVEYSFIEFVRMHYKTI 446

Query: 401 ASSLRDCVSIFCTHVSRSVLKCNFERMGCCLMSLIDSLQSLLFQNWVASEELKRVFSSN- 460
           + SL++C+SIFCTH+  ++LK NFER+  C+MSLI+S +SLL  N V S+EL+++F    
Sbjct: 447 SRSLKECISIFCTHIPIAILKHNFERLS-CVMSLINSFESLLLSNGVQSKELEKLFLKKV 506

Query: 461 QNDAYGSLFTKYEELLKTRNDCIVVLKSLQQSLDLLGLPQTTNKRTIVNFCFQNASLFFC 490
           + +       +YE+LLK RNDC++VL+SL+ SL  L LPQT++K  +  FCF+NASLFFC
Sbjct: 507 EEEVVEDQNVEYEKLLKGRNDCVLVLRSLEYSLSELRLPQTSSKGGLRKFCFRNASLFFC 566

BLAST of Lsi11G000340 vs. ExPASy TrEMBL
Match: A0A0A0K7S2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G276680 PE=4 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 9.1e-98
Identity = 239/558 (42.83%), Postives = 321/558 (57.53%), Query Frame = 0

Query: 41  LSSSMDPISKAPFTQVISLEEINPSELGLYNIQINKWQNGHKESSTL---GDIFILSDVK 100
           L SSM+PISK+P  +VIS++EI P   GL+NI I       K+  T+   G+IFILS+VK
Sbjct: 20  LCSSMNPISKSPCVEVISIKEIKPYGKGLFNIHIRDC----KDCPTILMPGNIFILSNVK 79

Query: 101 PNLVSDLRR----IGKTWTFASFFPNKNDDDNDNETFMSS-----FILKTWQPNFKM--- 160
             +VSDL R      K+WTFA+ F  K   +N  E F+S      F +KTW  +F++   
Sbjct: 80  SYVVSDLERDNNNNAKSWTFATKFWAKG--NNLEEEFLSDNDPTRFSVKTWNKDFEIPMD 139

Query: 161 --NSKQPIFLIYLVNILPYIRIWNALHM-------NNKNNSSIFNQILSSNNNNNNSSSN 220
                +P+FL+ LVN+L  IRIWNALHM       ++ ++S IFNQ+L   ++ NN   +
Sbjct: 140 EKTKNKPMFLVILVNVLSNIRIWNALHMIKRTSKHSSASSSMIFNQVLGLKDSCNNLDFS 199

Query: 221 RHCNERD--ELLSDTSSLFSMLNESQVKAIGSCLKTISCEDKCGIELIWGPPGTGKTITV 280
               E +     S    LFS LNE Q +A+  CL+  SC  K  IELIWGPPGTGKT TV
Sbjct: 200 CDACEAEVGMSFSHNDDLFSTLNEPQARAVQRCLEKASCAHKSSIELIWGPPGTGKTKTV 259

Query: 281 GILLFQLLRNQCRTIACAPTNTAIMQLASKFL---------------------------- 340
            +LL Q  +N  R + CAPTNTAIMQ+AS+ L                            
Sbjct: 260 AVLLLQFRKNNHRVLTCAPTNTAIMQVASRLLSLVKEMHEKEYGSGELFCNLSDILLIGN 319

Query: 341 -----------------------------------------------VLDVDD------- 400
                                                          V D D+       
Sbjct: 320 ETRLKLEECDKYIHLDYRVERLGKCFSQFSGWSHCFASMVDFLQGRCVFDYDEDQKGPKR 379

Query: 401 YMSFLEFVQTRFRVIASSLRDCVSIFCTHVSRSVLKCNFERMGCCLMSLIDSLQSLLFQN 460
           + +F+EFV+T+++ +A  L++C+SI CTH+ +++L  NFER+G CLMSL+DSL++ LF N
Sbjct: 380 FKNFIEFVRTQYKTLAYPLKECISILCTHIPKTILLHNFERLG-CLMSLMDSLEASLFSN 439

Query: 461 WVASEELKRVFSS--NQNDAYGSLFTKYEELLKTRNDCIVVLKSLQQSLDLLGLPQTTNK 488
           WV S   K++FS+   + +       +Y++LLK  NDC++VL SL+ SL  L LPQT+ K
Sbjct: 440 WVVS---KKLFSTKLEEKEEVMKNNDEYKKLLKEINDCVLVLNSLKHSLSRLKLPQTSCK 499

BLAST of Lsi11G000340 vs. ExPASy TrEMBL
Match: A0A6J1CTS8 (LOW QUALITY PROTEIN: ATP-dependent helicase NAM7-like OS=Momordica charantia OX=3673 GN=LOC111014153 PE=3 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 1.5e-97
Identity = 211/354 (59.60%), Postives = 249/354 (70.34%), Query Frame = 0

Query: 169 LHMNNKNNSSIFNQILSSNNNNNNSSSNRHCNERDELLSDTSSLFSMLNESQVKAIGSCL 228
           L     + ++I N+I S  ++++        +   +   D + LFS LNESQVKAI  CL
Sbjct: 3   LRKAKNSTANILNRIRSLGSDSSGCGVCEESDNDHDDDDDDNDLFSSLNESQVKAIRPCL 62

Query: 229 KTISCEDKCGIELIWGPPGTGKTITVGILLFQLLRNQCRTIACAPTNTAIMQLASKFLVL 288
           KT SC+ +  +ELIWGPPGTGKT TVG+LL +LL N+CRT+ACAPTNTA+MQ+AS+FLVL
Sbjct: 63  KTTSCDHR-SVELIWGPPGTGKTKTVGVLLLELLSNKCRTLACAPTNTAVMQMASRFLVL 122

Query: 289 ------------------------------------DVDDYMSFLEFVQTRFRVIASSLR 348
                                               DVDD MSFLEFV+ +FR ++SS R
Sbjct: 123 VREMHQKSEGIFCSLGDVLLFGNEKKLKNCVSHYRRDVDD-MSFLEFVRKQFRALSSSSR 182

Query: 349 DCVSIFCTHVSRSVLKCNFERMGCCLMSLIDSLQSLLFQNWVASEELKRVFSSNQNDAYG 408
           DCVSI CTHV +S+LK NF+R+  CLMSLIDSLQSLLF+N V  +EL+ V S        
Sbjct: 183 DCVSILCTHVPKSILKHNFQRLD-CLMSLIDSLQSLLFRNGVVLDELEEVVSDR---CPS 242

Query: 409 SLFTKYEELLKTRNDCIVVLKSLQQSLDLLGLPQTTNKRTIVNFCFQNASLFFCTVSSLF 468
            LF+K+E+LLK RNDC+VVLKSLQQSLD L LP TTNK TI NFCF+NASLFFCTVSS F
Sbjct: 243 RLFSKFEQLLKARNDCVVVLKSLQQSLDELRLPHTTNKATIANFCFRNASLFFCTVSSSF 302

Query: 469 KLYSKSLEPLKALVIDEAAQLKECESVIPMQLPDIMHAILIGDECQLPAMVESK 487
           KLYSKSL PLKA+VIDEAAQLKECESVIPMQLPDI H ILIGDECQLPAMVESK
Sbjct: 303 KLYSKSLAPLKAVVIDEAAQLKECESVIPMQLPDIKHVILIGDECQLPAMVESK 350

BLAST of Lsi11G000340 vs. ExPASy TrEMBL
Match: A0A5D3E3E0 (Regulator of nonsense transcripts 1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold349G00030 PE=4 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 3.2e-87
Identity = 194/318 (61.01%), Postives = 203/318 (63.84%), Query Frame = 0

Query: 215 MLNESQVKAIGSCLKTISCEDKCGIELIWGPPGTGKTITVGILLFQLLRNQCRTIACAPT 274
           MLNESQ KAI SCLKTISC+ KCGIELIWGPPGTGKT+TVGILLFQLLRNQCRT+ACAPT
Sbjct: 1   MLNESQTKAIASCLKTISCKHKCGIELIWGPPGTGKTMTVGILLFQLLRNQCRTVACAPT 60

Query: 275 NTAIMQLASKFLVL--------------------------------------DVD-DYMS 334
           NTAIMQLA+KFL L                                      DVD D MS
Sbjct: 61  NTAIMQLATKFLALVKQMHEKKLQECFNPFTGWRHCFASMTDFLEDCILQFQDVDNDDMS 120

Query: 335 FLEFVQTRFRVIASSLRDCVSIFCTHVSRSVLKCNFERMGCCLMSLIDSLQSLLFQNWVA 394
           FL+FVQTRF+VIASSLRDC+SIFCTHVSRSVLK NFE M  CLMSLIDS QSLLF+NWV 
Sbjct: 121 FLKFVQTRFKVIASSLRDCISIFCTHVSRSVLKYNFENMS-CLMSLIDSFQSLLFENWVE 180

Query: 395 SEELKRVFSSNQNDAYGSLFTKYEELLKTRNDCIVVLKSLQQSLDLLGLPQTTNKRTIVN 454
           SEEL+R                                                      
Sbjct: 181 SEELER------------------------------------------------------ 240

Query: 455 FCFQNASLFFCTVSSLFKLYSKSLEPLKALVIDEAAQLKECESVIPMQLPDIMHAILIGD 494
               NASLFFCTVS  FKLYSKSLEPLKALVIDEAAQLKECE VIPMQL DI HAILIGD
Sbjct: 241 ----NASLFFCTVSGSFKLYSKSLEPLKALVIDEAAQLKECECVIPMQLADIKHAILIGD 259

BLAST of Lsi11G000340 vs. NCBI nr
Match: XP_008461528.2 (PREDICTED: uncharacterized protein LOC103500100 [Cucumis melo])

HSP 1 Score: 411.8 bits (1057), Expect = 8.7e-111
Identity = 248/540 (45.93%), Postives = 331/540 (61.30%), Query Frame = 0

Query: 41  LSSSMDPISKAPFTQVISLEEINPSELGLYNIQINKWQNGHKESSTLGDIFILSDVKPNL 100
           L SSM+PISK+P  QVI+L+EI     GL+ I +   +         G+IFILS+VKP +
Sbjct: 52  LYSSMNPISKSPCVQVIALKEIKTYGKGLFEIHLKDCKQYCPTILIPGNIFILSNVKPKV 111

Query: 101 VSDLRRIGKTWTFASFFPNKNDDDNDNETFMSSFILKTWQPNFKM---NSKQPIFLIYLV 160
           VSDL+R G+TWTFA+F   K    + N+   + F +K W+ NF     + + P+FL+ LV
Sbjct: 112 VSDLQRNGRTWTFATFVSKKGKKKDKNKP--TCFTIKIWKENFMKDLNHHEHPMFLVVLV 171

Query: 161 NILPYIRIWNALHMNNKNNSSIFNQILSSNNNNNNSSSNRH-CNERDELLSDTSSLFSML 220
           N+L  IRIWNALHM  K N++IFN +L  N++N+N +     C  + + LS  +SLF  L
Sbjct: 172 NVLTNIRIWNALHM--KKNNAIFNHVLGVNSSNSNLNFGCDVCETKIDELSSNNSLFFTL 231

Query: 221 NESQVKAIGSCLKTISCEDKCGIELIWGPPGTGKTITVGILLFQLLRNQCRTIACAPTNT 280
           NESQ +A+G+CLK  SC  K G+ELIWGPPGTGKT TVG+LLF+L +   RT+ACAPTNT
Sbjct: 232 NESQARAVGTCLKRTSCAHKYGVELIWGPPGTGKTKTVGVLLFELRKKNRRTLACAPTNT 291

Query: 281 AIMQLASKFLVL------------------------------------DVDDYM------ 340
           AIMQ+AS+FL+L                                    D D Y+      
Sbjct: 292 AIMQVASRFLLLVKEMHGKKDNRSKKLFCNLGDILLFGNKERLKVGESDKDIYLDYRIGR 351

Query: 341 ---------------------------------------------SFLEFVQTRFRVIAS 400
                                                        SF+EFV+  ++ I+ 
Sbjct: 352 LEKCFNQFNGWRVCFSSMIDFLEGYCVSQYRAFLKDKQRPKKFEYSFIEFVRVYYKTISC 411

Query: 401 SLRDCVSIFCTHVSRSVLKCNFERMGCCLMSLIDSLQSLLFQNWVASEELKRVFSSN-QN 460
           SL++C+SIFCTH+  ++LK NFER+ CC+MSLI+S +SLL  N V S+EL+++FS   + 
Sbjct: 412 SLKECISIFCTHIPIAILKHNFERL-CCVMSLIESFESLLLSNGVESKELEKLFSKKVEE 471

Query: 461 DAYGSLFTKYEELLKTRNDCIVVLKSLQQSLDLLGLPQTTNKRTIVNFCFQNASLFFCTV 488
           +   +   +YE+LLK RNDC++VL+SL+ SL  L LPQT+ +R +  FCF+NASLFFCTV
Sbjct: 472 EVVVNQNVEYEKLLKGRNDCVLVLRSLKYSLGELKLPQTSRERRLRAFCFRNASLFFCTV 531

BLAST of Lsi11G000340 vs. NCBI nr
Match: XP_031745084.1 (uncharacterized protein LOC101214715 [Cucumis sativus])

HSP 1 Score: 401.7 bits (1031), Expect = 9.0e-108
Identity = 247/542 (45.57%), Postives = 325/542 (59.96%), Query Frame = 0

Query: 41  LSSSMDPISKAPFTQVISLEEINPSELGLYNIQINKWQNGHKESSTLGDIFILSDVKPNL 100
           L SSM+PISK+P  QVISL+EI P   GL+ I +   +         G+IFILS+VKP +
Sbjct: 84  LHSSMNPISKSPCVQVISLKEIKPYGKGLFQIHLKDCKQYCPTILIPGNIFILSNVKPKV 143

Query: 101 VSDLRRIGKTWTFA-SFFPNKNDDDNDNETFMSSFILKTWQPNFKM---NSKQPIFLIYL 160
           VSDL+  GKTWTFA +F   K      N+   + F +KTW+ NF     + + P FL+ L
Sbjct: 144 VSDLQGNGKTWTFATTFVSQKGKKKGTNKP--TCFTIKTWKENFMKDLNHHEHPTFLVVL 203

Query: 161 VNILPYIRIWNALHMNNKNNSSIFNQILSSNNNNNNSSSNRH-CNERDELLSDTSSLFSM 220
           VN+L  +RIWNALHM  K N++IFN +L   + N+N +     C  + E LS  SSLF  
Sbjct: 204 VNVLTNVRIWNALHM--KKNNAIFNHVLGVTSFNSNLNFGCDVCETKIEELSSKSSLFCT 263

Query: 221 LNESQVKAIGSCLKTISCEDKCGIELIWGPPGTGKTITVGILLFQLLRNQCRTIACAPTN 280
           LNESQ +A+G+CL  ISC  K G+ELIWGPPGTGKT TVG+LLF+L +   RT+ACAPTN
Sbjct: 264 LNESQARAVGTCLNRISCVHKYGVELIWGPPGTGKTKTVGVLLFELRKKNRRTLACAPTN 323

Query: 281 TAIMQLASKFLVL----------------------------------DVDDYM------- 340
           TAIMQ+AS+FL+L                                  + D Y+       
Sbjct: 324 TAIMQVASRFLLLVKEMHDKKDNGSKGLFCNLGDILLFGNKERLKVGESDKYIYLDYRIG 383

Query: 341 -----------------------------------------------SFLEFVQTRFRVI 400
                                                          SF+EFV+  ++ I
Sbjct: 384 RLKKCFNQLNGWRFCFSSMIDFLEGHCVSQYRTFLKDHKQRSKMVEYSFIEFVRMHYKTI 443

Query: 401 ASSLRDCVSIFCTHVSRSVLKCNFERMGCCLMSLIDSLQSLLFQNWVASEELKRVFSSN- 460
           + SL++C+SIFCTH+  ++LK NFER+  C+MSLI+S +SLL  N V S+EL+++F    
Sbjct: 444 SRSLKECISIFCTHIPIAILKHNFERLS-CVMSLINSFESLLLSNGVQSKELEKLFLKKV 503

Query: 461 QNDAYGSLFTKYEELLKTRNDCIVVLKSLQQSLDLLGLPQTTNKRTIVNFCFQNASLFFC 488
           + +       +YE+LLK RNDC++VL+SL+ SL  L LPQT++K  +  FCF+NASLFFC
Sbjct: 504 EEEVVEDQNVEYEKLLKGRNDCVLVLRSLEYSLSELRLPQTSSKGGLRKFCFRNASLFFC 563

BLAST of Lsi11G000340 vs. NCBI nr
Match: KGN44700.2 (hypothetical protein Csa_016709 [Cucumis sativus])

HSP 1 Score: 401.7 bits (1031), Expect = 9.0e-108
Identity = 247/542 (45.57%), Postives = 325/542 (59.96%), Query Frame = 0

Query: 41  LSSSMDPISKAPFTQVISLEEINPSELGLYNIQINKWQNGHKESSTLGDIFILSDVKPNL 100
           L SSM+PISK+P  QVISL+EI P   GL+ I +   +         G+IFILS+VKP +
Sbjct: 70  LHSSMNPISKSPCVQVISLKEIKPYGKGLFQIHLKDCKQYCPTILIPGNIFILSNVKPKV 129

Query: 101 VSDLRRIGKTWTFA-SFFPNKNDDDNDNETFMSSFILKTWQPNFKM---NSKQPIFLIYL 160
           VSDL+  GKTWTFA +F   K      N+   + F +KTW+ NF     + + P FL+ L
Sbjct: 130 VSDLQGNGKTWTFATTFVSQKGKKKGTNKP--TCFTIKTWKENFMKDLNHHEHPTFLVVL 189

Query: 161 VNILPYIRIWNALHMNNKNNSSIFNQILSSNNNNNNSSSNRH-CNERDELLSDTSSLFSM 220
           VN+L  +RIWNALHM  K N++IFN +L   + N+N +     C  + E LS  SSLF  
Sbjct: 190 VNVLTNVRIWNALHM--KKNNAIFNHVLGVTSFNSNLNFGCDVCETKIEELSSKSSLFCT 249

Query: 221 LNESQVKAIGSCLKTISCEDKCGIELIWGPPGTGKTITVGILLFQLLRNQCRTIACAPTN 280
           LNESQ +A+G+CL  ISC  K G+ELIWGPPGTGKT TVG+LLF+L +   RT+ACAPTN
Sbjct: 250 LNESQARAVGTCLNRISCVHKYGVELIWGPPGTGKTKTVGVLLFELRKKNRRTLACAPTN 309

Query: 281 TAIMQLASKFLVL----------------------------------DVDDYM------- 340
           TAIMQ+AS+FL+L                                  + D Y+       
Sbjct: 310 TAIMQVASRFLLLVKEMHDKKDNGSKGLFCNLGDILLFGNKERLKVGESDKYIYLDYRIG 369

Query: 341 -----------------------------------------------SFLEFVQTRFRVI 400
                                                          SF+EFV+  ++ I
Sbjct: 370 RLKKCFNQLNGWRFCFSSMIDFLEGHCVSQYRTFLKDHKQRSKMVEYSFIEFVRMHYKTI 429

Query: 401 ASSLRDCVSIFCTHVSRSVLKCNFERMGCCLMSLIDSLQSLLFQNWVASEELKRVFSSN- 460
           + SL++C+SIFCTH+  ++LK NFER+  C+MSLI+S +SLL  N V S+EL+++F    
Sbjct: 430 SRSLKECISIFCTHIPIAILKHNFERLS-CVMSLINSFESLLLSNGVQSKELEKLFLKKV 489

Query: 461 QNDAYGSLFTKYEELLKTRNDCIVVLKSLQQSLDLLGLPQTTNKRTIVNFCFQNASLFFC 488
           + +       +YE+LLK RNDC++VL+SL+ SL  L LPQT++K  +  FCF+NASLFFC
Sbjct: 490 EEEVVEDQNVEYEKLLKGRNDCVLVLRSLEYSLSELRLPQTSSKGGLRKFCFRNASLFFC 549

BLAST of Lsi11G000340 vs. NCBI nr
Match: XP_011659750.2 (uncharacterized protein LOC101220392 [Cucumis sativus])

HSP 1 Score: 367.5 bits (942), Expect = 1.9e-97
Identity = 239/558 (42.83%), Postives = 321/558 (57.53%), Query Frame = 0

Query: 41  LSSSMDPISKAPFTQVISLEEINPSELGLYNIQINKWQNGHKESSTL---GDIFILSDVK 100
           L SSM+PISK+P  +VIS++EI P   GL+NI I       K+  T+   G+IFILS+VK
Sbjct: 108 LCSSMNPISKSPCVEVISIKEIKPYGKGLFNIHIRDC----KDCPTILMPGNIFILSNVK 167

Query: 101 PNLVSDLRR----IGKTWTFASFFPNKNDDDNDNETFMSS-----FILKTWQPNFKM--- 160
             +VSDL R      K+WTFA+ F  K   +N  E F+S      F +KTW  +F++   
Sbjct: 168 SYVVSDLERDNNNNAKSWTFATKFWAKG--NNLEEEFLSDNDPTRFSVKTWNKDFEIPMD 227

Query: 161 --NSKQPIFLIYLVNILPYIRIWNALHM-------NNKNNSSIFNQILSSNNNNNNSSSN 220
                +P+FL+ LVN+L  IRIWNALHM       ++ ++S IFNQ+L   ++ NN   +
Sbjct: 228 EKTKNKPMFLVILVNVLSNIRIWNALHMIKRTSKHSSASSSMIFNQVLGLKDSCNNLDFS 287

Query: 221 RHCNERD--ELLSDTSSLFSMLNESQVKAIGSCLKTISCEDKCGIELIWGPPGTGKTITV 280
               E +     S    LFS LNE Q +A+  CL+  SC  K  IELIWGPPGTGKT TV
Sbjct: 288 CDACEAEVGMSFSHNDDLFSTLNEPQARAVQRCLEKASCAHKSSIELIWGPPGTGKTKTV 347

Query: 281 GILLFQLLRNQCRTIACAPTNTAIMQLASKFL---------------------------- 340
            +LL Q  +N  R + CAPTNTAIMQ+AS+ L                            
Sbjct: 348 AVLLLQFRKNNHRVLTCAPTNTAIMQVASRLLSLVKEMHEKEYGSGELFCNLSDILLIGN 407

Query: 341 -----------------------------------------------VLDVDD------- 400
                                                          V D D+       
Sbjct: 408 ETRLKLEECDKYIHLDYRVERLGKCFSQFSGWSHCFASMVDFLQGRCVFDYDEDQKGPKR 467

Query: 401 YMSFLEFVQTRFRVIASSLRDCVSIFCTHVSRSVLKCNFERMGCCLMSLIDSLQSLLFQN 460
           + +F+EFV+T+++ +A  L++C+SI CTH+ +++L  NFER+G CLMSL+DSL++ LF N
Sbjct: 468 FKNFIEFVRTQYKTLAYPLKECISILCTHIPKTILLHNFERLG-CLMSLMDSLEASLFSN 527

Query: 461 WVASEELKRVFSS--NQNDAYGSLFTKYEELLKTRNDCIVVLKSLQQSLDLLGLPQTTNK 488
           WV S   K++FS+   + +       +Y++LLK  NDC++VL SL+ SL  L LPQT+ K
Sbjct: 528 WVVS---KKLFSTKLEEKEEVMKNNDEYKKLLKEINDCVLVLNSLKHSLSRLKLPQTSCK 587

BLAST of Lsi11G000340 vs. NCBI nr
Match: XP_022144472.1 (LOW QUALITY PROTEIN: ATP-dependent helicase NAM7-like [Momordica charantia])

HSP 1 Score: 366.7 bits (940), Expect = 3.2e-97
Identity = 211/354 (59.60%), Postives = 249/354 (70.34%), Query Frame = 0

Query: 169 LHMNNKNNSSIFNQILSSNNNNNNSSSNRHCNERDELLSDTSSLFSMLNESQVKAIGSCL 228
           L     + ++I N+I S  ++++        +   +   D + LFS LNESQVKAI  CL
Sbjct: 3   LRKAKNSTANILNRIRSLGSDSSGCGVCEESDNDHDDDDDDNDLFSSLNESQVKAIRPCL 62

Query: 229 KTISCEDKCGIELIWGPPGTGKTITVGILLFQLLRNQCRTIACAPTNTAIMQLASKFLVL 288
           KT SC+ +  +ELIWGPPGTGKT TVG+LL +LL N+CRT+ACAPTNTA+MQ+AS+FLVL
Sbjct: 63  KTTSCDHR-SVELIWGPPGTGKTKTVGVLLLELLSNKCRTLACAPTNTAVMQMASRFLVL 122

Query: 289 ------------------------------------DVDDYMSFLEFVQTRFRVIASSLR 348
                                               DVDD MSFLEFV+ +FR ++SS R
Sbjct: 123 VREMHQKSEGIFCSLGDVLLFGNEKKLKNCVSHYRRDVDD-MSFLEFVRKQFRALSSSSR 182

Query: 349 DCVSIFCTHVSRSVLKCNFERMGCCLMSLIDSLQSLLFQNWVASEELKRVFSSNQNDAYG 408
           DCVSI CTHV +S+LK NF+R+  CLMSLIDSLQSLLF+N V  +EL+ V S        
Sbjct: 183 DCVSILCTHVPKSILKHNFQRLD-CLMSLIDSLQSLLFRNGVVLDELEEVVSDR---CPS 242

Query: 409 SLFTKYEELLKTRNDCIVVLKSLQQSLDLLGLPQTTNKRTIVNFCFQNASLFFCTVSSLF 468
            LF+K+E+LLK RNDC+VVLKSLQQSLD L LP TTNK TI NFCF+NASLFFCTVSS F
Sbjct: 243 RLFSKFEQLLKARNDCVVVLKSLQQSLDELRLPHTTNKATIANFCFRNASLFFCTVSSSF 302

Query: 469 KLYSKSLEPLKALVIDEAAQLKECESVIPMQLPDIMHAILIGDECQLPAMVESK 487
           KLYSKSL PLKA+VIDEAAQLKECESVIPMQLPDI H ILIGDECQLPAMVESK
Sbjct: 303 KLYSKSLAPLKAVVIDEAAQLKECESVIPMQLPDIKHVILIGDECQLPAMVESK 350

BLAST of Lsi11G000340 vs. TAIR 10
Match: AT1G65810.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 163.7 bits (413), Expect = 3.8e-40
Identity = 155/533 (29.08%), Postives = 237/533 (44.47%), Query Frame = 0

Query: 41  LSSSMDPISKA---PFTQVISLEEINPSELGLYNIQI---NKWQ-NGHKESSTLGDIFIL 100
           L SSM  I +A    F ++   ++  P     Y + +   N++   G +    + D+  +
Sbjct: 73  LLSSMGTIRRAQAFKFWEIKPGKDFKPPRDLYYEVTLQMTNEYMTKGGQNLLEVNDLIAV 132

Query: 101 SDVKPNLVSDLRRIGKTWTFASFFPNKNDDDNDNETFMSSFILKTWQPNFKMNSKQ---- 160
           +D +P  + DLR   + +  A       ++ +      S  I+     + K +SK+    
Sbjct: 133 TDKRPIRIDDLRFSHEPYLLALVCGVNENNPHLITILASKPIIFDDDDDIKTSSKRGKGE 192

Query: 161 ----PIFLIYLVNILPYIRIWNALHMNNK-NNSSIFNQILSSNNNNNNSSSNRHCNERDE 220
                 F + L+N++  IRIW ALH N +  N  + +++L SNN  +  S        + 
Sbjct: 193 RKSLSFFGVNLINMMTNIRIWTALHPNPEGGNLKLISRVLQSNNEVDGGSCVSCKENSES 252

Query: 221 LLSDTSSLF---SMLNESQVKAIGSCLKTISCEDKCGIELIWGPPGTGKTITVGILLFQL 280
           ++SD S+       LN SQ  AI  CL+  SC     I+LIWGPPGTGKT T  +LL   
Sbjct: 253 VVSDYSARMLRSFKLNSSQEDAILRCLEAKSCNHSNNIKLIWGPPGTGKTKTTSVLLLNF 312

Query: 281 LRNQCRTIACAPTNTAIMQLASKFLVLDVDDYMSF-------LEFVQTRFRVIASSLRDC 340
           L+ +CRT+ CAPTN A++++ S+ + L V + + F       +     + R+      D 
Sbjct: 313 LKMRCRTLTCAPTNIAVLEVCSRLVKL-VSESLRFDGYGLGDIVLFGNKERMKIDDREDL 372

Query: 341 VSIFCTHVSRSVLKC---------NFERMGCCLMSLIDSLQS--------LLFQNWVASE 400
             +F  +    + +C         N  RM C L       +         L F+++V  E
Sbjct: 373 FDVFLEYRVDELYRCFMALTGWRANVNRMICLLSDPKHEFRQFKSVNTTLLSFKDFV-EE 432

Query: 401 ELKRV---------------------------------------FSSNQNDAYGSLFTKY 460
            L R+                                        S    D YG +  K 
Sbjct: 433 RLSRLRYDLHHQFTTLCLHLPTSLLSFRVAEKMNQTNNLLRNIAASDVMRDGYGRMKYKL 492

Query: 461 ----EELLKTRNDCIVVLKSLQQSLDLLGLPQTTNKRTIVNFCFQNASLFFCTVSSLFKL 488
               +E      DC+ +L S+  S+    LP   +K  +   C  NA L FCT SS  +L
Sbjct: 493 KDTGDENDSRTQDCLEMLTSISMSIK---LPDFISKFELQKLCLDNAYLLFCTASSSARL 552

BLAST of Lsi11G000340 vs. TAIR 10
Match: AT1G65810.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 163.7 bits (413), Expect = 3.8e-40
Identity = 155/533 (29.08%), Postives = 237/533 (44.47%), Query Frame = 0

Query: 41  LSSSMDPISKA---PFTQVISLEEINPSELGLYNIQI---NKWQ-NGHKESSTLGDIFIL 100
           L SSM  I +A    F ++   ++  P     Y + +   N++   G +    + D+  +
Sbjct: 73  LLSSMGTIRRAQAFKFWEIKPGKDFKPPRDLYYEVTLQMTNEYMTKGGQNLLEVNDLIAV 132

Query: 101 SDVKPNLVSDLRRIGKTWTFASFFPNKNDDDNDNETFMSSFILKTWQPNFKMNSKQ---- 160
           +D +P  + DLR   + +  A       ++ +      S  I+     + K +SK+    
Sbjct: 133 TDKRPIRIDDLRFSHEPYLLALVCGVNENNPHLITILASKPIIFDDDDDIKTSSKRGKGE 192

Query: 161 ----PIFLIYLVNILPYIRIWNALHMNNK-NNSSIFNQILSSNNNNNNSSSNRHCNERDE 220
                 F + L+N++  IRIW ALH N +  N  + +++L SNN  +  S        + 
Sbjct: 193 RKSLSFFGVNLINMMTNIRIWTALHPNPEGGNLKLISRVLQSNNEVDGGSCVSCKENSES 252

Query: 221 LLSDTSSLF---SMLNESQVKAIGSCLKTISCEDKCGIELIWGPPGTGKTITVGILLFQL 280
           ++SD S+       LN SQ  AI  CL+  SC     I+LIWGPPGTGKT T  +LL   
Sbjct: 253 VVSDYSARMLRSFKLNSSQEDAILRCLEAKSCNHSNNIKLIWGPPGTGKTKTTSVLLLNF 312

Query: 281 LRNQCRTIACAPTNTAIMQLASKFLVLDVDDYMSF-------LEFVQTRFRVIASSLRDC 340
           L+ +CRT+ CAPTN A++++ S+ + L V + + F       +     + R+      D 
Sbjct: 313 LKMRCRTLTCAPTNIAVLEVCSRLVKL-VSESLRFDGYGLGDIVLFGNKERMKIDDREDL 372

Query: 341 VSIFCTHVSRSVLKC---------NFERMGCCLMSLIDSLQS--------LLFQNWVASE 400
             +F  +    + +C         N  RM C L       +         L F+++V  E
Sbjct: 373 FDVFLEYRVDELYRCFMALTGWRANVNRMICLLSDPKHEFRQFKSVNTTLLSFKDFV-EE 432

Query: 401 ELKRV---------------------------------------FSSNQNDAYGSLFTKY 460
            L R+                                        S    D YG +  K 
Sbjct: 433 RLSRLRYDLHHQFTTLCLHLPTSLLSFRVAEKMNQTNNLLRNIAASDVMRDGYGRMKYKL 492

Query: 461 ----EELLKTRNDCIVVLKSLQQSLDLLGLPQTTNKRTIVNFCFQNASLFFCTVSSLFKL 488
               +E      DC+ +L S+  S+    LP   +K  +   C  NA L FCT SS  +L
Sbjct: 493 KDTGDENDSRTQDCLEMLTSISMSIK---LPDFISKFELQKLCLDNAYLLFCTASSSARL 552

BLAST of Lsi11G000340 vs. TAIR 10
Match: AT5G37150.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 162.5 bits (410), Expect = 8.5e-40
Identity = 154/564 (27.30%), Postives = 236/564 (41.84%), Query Frame = 0

Query: 32  HSPINVSLSLSSSMDPISKAPFTQVISLEEINPSELG------LYNIQINKWQN-GHKES 91
           H  I     L SS+  +SK+PF Q+ S+E       G       Y+I +   ++   K  
Sbjct: 49  HLLIEAHTELFSSLKSVSKSPFVQIRSMETKTKQSSGSSSNKLFYDITLKATESLSAKYQ 108

Query: 92  STLGDIFILSDVKPNLVSDLRRIGKTWTFASFFPNKNDDDNDNETFMSSFILKTWQPNFK 151
              GD+  L+  KP  ++DL  +   + F+S          D +  +S  + ++  P   
Sbjct: 109 PKCGDLIALTMDKPRRINDLNPLLLAYVFSS----------DGDLKISVHLSRSISP--- 168

Query: 152 MNSKQPIFLIYLVNILPYIRIWNALHMNNKNNSSIFNQILSSNNNNNNSSSNRHCNERDE 211
              +   F ++L+ +    RIWNALH N    S++   +L +N  NN            +
Sbjct: 169 --LENYSFGVFLMTLTTNTRIWNALH-NEAAISTLTKSVLQANTVNNVFVL--------K 228

Query: 212 LLSDTSSLFSM-----LNESQVKAIGSCLKTISCEDKCGIELIWGPPGTGKTITVGILLF 271
           ++ D +    +     LN SQ  AI  CL+T +C  K  ++LIWGPPGTGKT TV  LLF
Sbjct: 229 MMGDLTLFLDIIRSTKLNSSQEDAILGCLETRNCTHKNSVKLIWGPPGTGKTKTVATLLF 288

Query: 272 QLLRNQCRTIACAPTNTAIMQLASKFL--------------------------------- 331
            LL+ +C+T+ CAPTNTAI+Q+AS+ L                                 
Sbjct: 289 ALLKLRCKTVVCAPTNTAIVQVASRLLSLFKENSTSENATYRLGNIILSGNRDRMGIHKN 348

Query: 332 ---VLDV-----------------------DDYMSFL----------------------- 391
              +LDV                       +  + FL                       
Sbjct: 349 DHVLLDVFLDERIGKLGKLFSPFSGWMQRLESLIQFLENPEGKYERHVYELEEVERMEEE 408

Query: 392 --------------EFVQTRFRVIASSLRDCVSIFCTHVSRSVLKCNFERMGCCLMSLID 451
                         EFV+  F  ++  +  C+    TH+ +  L  +  ++      +I 
Sbjct: 409 AERQEVVVNIPTIGEFVKKNFNSLSEEVETCIVDLFTHLPKVYLPYDDVKI------MIA 468

Query: 452 SLQSLLFQNWVASEELKRVFSSNQNDAYGSLFTKYEELLKTRNDCIVVLKSLQQSLDLLG 488
           S QSL    +   E   RV     N        +++   +   DC+  L+ L +  +   
Sbjct: 469 SRQSLQRIRYFLRENSSRVDFEEGN-------FRFDCFKRLSVDCLKALRLLPKRFE--- 528

BLAST of Lsi11G000340 vs. TAIR 10
Match: AT1G65780.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 151.0 bits (380), Expect = 2.6e-36
Identity = 154/566 (27.21%), Postives = 232/566 (40.99%), Query Frame = 0

Query: 40  SLSSSMDPISKAPFTQVI----SLEEINPSELGLYNIQINKWQNGHKESSTLGDIFILSD 99
           +L SSM  + +AP  ++     + E   P++L  Y ++++   N         D+  L+D
Sbjct: 61  ALLSSMRKLWQAPVVEISYIMQTAEYKLPNDL-FYKVRLSGISNEASTKLMPRDLISLTD 120

Query: 100 VKPNLVSDLRRIGKTWTFASFFPNKNDDDNDNETFMSSFILKTWQPNFKMNSKQPIFLIY 159
            +PN V       + +  A       D  ND     S  +        K   K+ +F I+
Sbjct: 121 QRPNHVDGFNISSEPYIVALVCKVDPDRPNDVTILASKPLFVEDGRRKKNEKKERLFGIH 180

Query: 160 LVNILPYIRIWNALHMNNKN-NSSIFNQILSSNNNNNNSSSNRHCNERDELLSD--TSSL 219
           LVN+   IRIWNALH  ++  N ++ +++L       NS     C +  +  SD      
Sbjct: 181 LVNLTTNIRIWNALHPGDEGVNLNLISRVL-----RRNSEDEGFCIQCLQEGSDGLAPRR 240

Query: 220 FSMLNESQVKAIGSCLKTISCEDKCGIELIWGPPGTGKTITVGILLFQLLRNQCRTIACA 279
           F  LN SQ  AI +CL    C     + LIWGPPGTGKT T  +LLF LL  +CRT+ C 
Sbjct: 241 FLKLNPSQEDAILNCLDVRRCYHANTVRLIWGPPGTGKTKTTSVLLFTLLNAKCRTLTCG 300

Query: 280 PTNTAIMQLASKFLVL-------------DV-----DDYM-------------------- 339
           PTN +++++AS+ L L             DV     D+ M                    
Sbjct: 301 PTNVSVLEVASRVLKLVSGSLKIGNYGLGDVVLFGNDERMKIKDRKDLVNIFIDERVDKL 360

Query: 340 ------------------------------------------------------------ 399
                                                                       
Sbjct: 361 YPCFMPFYGWKATIDGMIRLLEDPKGQYNLYLENLARANNVKRKDTGSVFKRKGNEQNEN 420

Query: 400 -----------SFLEFVQTRFRVIASSLRDCVSIFCTHVSRSVLKCNFERMGCCLMSLID 459
                      SF +++  +F  +   L    S  CTH+  ++L           + L+ 
Sbjct: 421 IVEQVSDTRPQSFQDYLPEKFSELRKDLDLHFSSLCTHLPTALLSSQAATRMYEAIDLVR 480

Query: 460 SLQSLLFQNWVASEELKRVFSSN--QNDAYGSLFTKYEELLKTRNDCIVVLKSLQQSLDL 488
            +  L   + V  E +K V   N   +D + S     E+      D + +L+S+    ++
Sbjct: 481 DVTILAILDGVTGEGVKSVLIPNGEGSDRFSSQHVTVED------DYLKLLRSIP---EI 540

BLAST of Lsi11G000340 vs. TAIR 10
Match: AT5G52090.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 139.8 bits (351), Expect = 5.9e-33
Identity = 122/420 (29.05%), Postives = 178/420 (42.38%), Query Frame = 0

Query: 164 RIWNALHMNNKNNSSIFNQILSSNNNNNNSSSNRHCNERDELLSDTSSLFSMLNESQVKA 223
           RIWNALH N  + S++   +L +N            + R +L+ D     + LN SQ  A
Sbjct: 8   RIWNALH-NEADISTLTKSVLQANTEGTEQCFCSENDGRSDLVLDIIR-STKLNSSQEDA 67

Query: 224 IGSCLKTISCEDKCGIELIWGPPGTGKTITVGILLFQLLRNQCRTIACAPTNTAIMQLAS 283
           I  CL+T +C  K  ++LIWGPP TGKT TV  LLF LL+ +C+T+ CAPTNTAI+Q+ S
Sbjct: 68  ILGCLETRNCTHKNSVKLIWGPPRTGKTKTVATLLFALLKLRCKTVVCAPTNTAIVQVTS 127

Query: 284 KFL------------------------------------VLDV----------------- 343
           + L                                    +LDV                 
Sbjct: 128 RLLSLFKENSTAENATYRLGNIILSGNRDRMGINKNDHVLLDVFLDERIGKLGKLFSPFS 187

Query: 344 ------DDYMSFL-------------------------------------EFVQTRFRVI 403
                 +  + FL                                     EFVQ  F  +
Sbjct: 188 GWMQRLESLIQFLENPEGKYERHVYELEEVERMNEEDEREEVVVNIPTFGEFVQKNFNSL 247

Query: 404 ASSLRDCVSIFCTHVSRSVLKCNFERMGCCLMSLIDSLQSLLFQNWVASEELKRVFSSNQ 463
           +  ++ C+    TH+ +  L          +  +I S Q+L    +   E   RV     
Sbjct: 248 SEEVKTCIVDLYTHLPKVYLPYE------DVKKMIASRQTLQRIRYFLRENSSRVDFEEG 307

Query: 464 NDAYGSLFTKYEELLKTRNDCIVVLKSLQQSLDLLGLPQTTNKRTIVNFCFQNASLFFCT 488
           N        +++   +  +DC+  L+ L +  +   +P       I  FC QNA +  CT
Sbjct: 308 N-------FRFDCFKRLSDDCLKALRLLPKRFE---IPDMLENEDIRKFCLQNADIILCT 367

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FJR04.6e-0642.86Regulator of nonsense transcripts 1 homolog OS=Arabidopsis thaliana OX=3702 GN=U... [more]
P307713.0e-0538.36ATP-dependent helicase NAM7 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S2... [more]
Q9HEH11.5e-0437.14Regulator of nonsense transcripts 1 homolog OS=Neurospora crassa (strain ATCC 24... [more]
Q929001.9e-0440.00Regulator of nonsense transcripts 1 OS=Homo sapiens OX=9606 GN=UPF1 PE=1 SV=2[more]
Q9EPU01.9e-0440.00Regulator of nonsense transcripts 1 OS=Mus musculus OX=10090 GN=Upf1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A1S3CEY44.2e-11145.93uncharacterized protein LOC103500100 OS=Cucumis melo OX=3656 GN=LOC103500100 PE=... [more]
A0A0A0K4155.1e-10945.59AAA_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G291110 PE=4... [more]
A0A0A0K7S29.1e-9842.83Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G276680 PE=4 SV=1[more]
A0A6J1CTS81.5e-9759.60LOW QUALITY PROTEIN: ATP-dependent helicase NAM7-like OS=Momordica charantia OX=... [more]
A0A5D3E3E03.2e-8761.01Regulator of nonsense transcripts 1-like protein OS=Cucumis melo var. makuwa OX=... [more]
Match NameE-valueIdentityDescription
XP_008461528.28.7e-11145.93PREDICTED: uncharacterized protein LOC103500100 [Cucumis melo][more]
XP_031745084.19.0e-10845.57uncharacterized protein LOC101214715 [Cucumis sativus][more]
KGN44700.29.0e-10845.57hypothetical protein Csa_016709 [Cucumis sativus][more]
XP_011659750.21.9e-9742.83uncharacterized protein LOC101220392 [Cucumis sativus][more]
XP_022144472.13.2e-9759.60LOW QUALITY PROTEIN: ATP-dependent helicase NAM7-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G65810.13.8e-4029.08P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G65810.23.8e-4029.08P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G37150.18.5e-4027.30P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G65780.12.6e-3627.21P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT5G52090.15.9e-3329.05P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 133..494
e-value: 1.3E-38
score: 135.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 147..482
IPR041677DNA2/NAM7 helicase, helicase domainPFAMPF13086AAA_11coord: 216..486
e-value: 2.6E-27
score: 96.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 13..32
NoneNo IPR availablePANTHERPTHR10887:SF474OS04G0582700 PROTEINcoord: 40..290
NoneNo IPR availablePANTHERPTHR10887:SF474OS04G0582700 PROTEINcoord: 307..489
IPR045055DNA2/NAM7-like helicasePANTHERPTHR10887DNA2/NAM7 HELICASE FAMILYcoord: 40..290
IPR045055DNA2/NAM7-like helicasePANTHERPTHR10887DNA2/NAM7 HELICASE FAMILYcoord: 307..489

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi11G000340.1Lsi11G000340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009987 cellular process
cellular_component GO:0005622 intracellular anatomical structure
cellular_component GO:0043226 organelle
molecular_function GO:0005488 binding
molecular_function GO:0004386 helicase activity