Lsi10G014940 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G014940
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionCalcium uniporter protein
Locationchr10: 19132436 .. 19136755 (-)
RNA-Seq ExpressionLsi10G014940
SyntenyLsi10G014940
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAAAAAGGTAATTATATATATATTATTTTGGTGCAACTGCGAAATAAAAATAAAATAAAATCTCCATTTTTGGAAGCAGCAAAGCGTCTAACGCGGTCCAAAAACAAAATAAAACAACCAACACGAAAGAGCCGCGTAGGGTTCTCGACCAAGCCCTTCCACGCCATTGCCTTGCCATTGCCGTTTCCCTCGGGACCTTCAAATCAACGCCTTAAAGCGCCAAACAAACCAATATAAACATTGCCCACTCCTGTCACATTGCTTCCTTTATTACATTCACTCTCTCAAATCAAATCAATTTCTTCTTCCACTCTCTTTCAATGGACGTTATGGAAGATTGCAGAATCATCGATGGAAGAATTTTTGGATTCCACGTTTGAGGATTATTACTGGAGGTTTCTGTTTCTGTTTTTGTTTTTGTTTTCTTTTTCAGCTTTTGAAGACGGGATGATGTGGAGGAAATGGTGTTCTTGTGCTAGTTTATTGAGTCGGAGATTGTTGGTGAATGGAGTTCCGGGGAATTCTTCTCCGGTACTTTCTTATTATGGAACTTTAGGGCTTGGTGGAGTTTCTTTGATTCCATCGACGGCGGTACGGTCATCGACCTGGTTTTGTACCAGAGTGGCTTCGTTTTCGTCGTCTGTGGATTCAGGAAATTCTACAGGAGTTCTTCGCGATGTGGGAAAAAGTGAGAGTGGTGGCCCTGGTCCTGGCCTTGGCCTTGGGTCTGTGAATGAAGAATCTGGTGATGGAATTGGTGGAGAGACGATTTCGTTTGGGGAAGCTAAAAGGCTGATGAGATTGGTTAATGTTGAGGCGCTGAAGATGAAATTGGGGACTGATGGGAAGGAGGCGATTGGATATTCGGAGCTTCTTGAAGCTTGTAAAAGCATGGGCGTTGCTAGGTCTCAGGATGAAGCTGCTGCTTTTGCTCGAGTTCTTGATGAAGCTGGTGTGGTTCTTCTTTTTCGCGATAAAGTCTATCTTCATCCGGACAAGGTTGGTGAAAGGGGTGGGACCTTATTCAATTAATGTACTGCATTGCATCATGGTTGTGGGTGTTGCATTTCTTCATAAATCGTTGTCATTCAATTTCTGGTTTATTTAGTGTAAAAAATACGGAAGGTAGGTAGAAGTTTTCTTAGAAAGGGATAGAGTTTTATGTACTGACTTTCTTTAGATGTGTAGGTTTTTCAATTTTAAGGACAGTTTGGGGACGAGTGGTTAAGGCGTGTGAACATGATCTATTCTATAGGTTGTACAACGGTGTCCTTAAGTGAAAAAAAAAAAAAAGAACAGTTTGGGGACAGAGTAGGGAAATTTTTTAATTTTGAATATAATTTTTTTCTTTTGAATCTAAGACAAATCAAAGAGGGACCATGGATGTAAGAAAGAAAGGCATTGGTGGGATGGTTCAATCAATGGTGGCGGACCATGATTTAATTGCGTCAAGTCAAATGGGTTGTCTCGTGAGATTTAGGGTACGTTCAAGCTGGGTCCATACACTTGAGAAAAATAAAAAAGAAGACAAATCAAAGGGGAAAGTTTGGAAGTTCTGGTTGTTTTTGACTTTTGTTTTGTTTTTTTTGTTCTCTTAAAAGGTTTAGAGAATGGAACATGTCATTCGGCTAATAAGATTTGGGGGGCTAACTAGGTCGACAGGATCCGGCATTTAGAAGTCTTTGAATGGATTTTCCTTCCTTAATGGAAAAACTGGCTTTTCATTAAGTCTTTGAATGGATTTTCCTTCCTTAATGGAAAAACTGGCTTTTCATTAAGTTGAATGGAAGGAAAAAAATAAAAAAGAAGGGTCGACCAAAAGGAGAAACACACAAAAAACACAACTACAAGTTGGTGACCAGCTATTGGTTATCAAATGGCACTTTTCAGAATTGGGCACAAAAAAAGAAGGCCAACTTCCACAAAAACATTGAAGAGGTAGAGAGGAAAATCAAATACTAATTAAGATTACTGTGATGGGAAGGGTAGTTTTTGGAGTTTTAAGACAAGTTTTGAGGACTTGCAATTTATACTTTCACAAGAAACGATCTGAGATATTATTTTGTTCTCTTCCCCTTAAGCGGGACGTGCTTATTGGCTCTGAGATGAATTTCAGAGATGTAGCTTATGCTTTTTTTTCTTTTTCATAAAGAATATCTTTGTGGGTCACAACTTTGTCCCCAATCTTAAACTGCCAAGAGTCAATAGTTTGGGCAGAGATATTGTTTATTTATTTTTGAAGGAGACAAACTTCCCATTGGTTTAGTTAAAAGTTTCATCTACTCTAGAAGAAAACCTACTAAGAGCATCTAAATTTGTGTTAACTACAGAAGGAATAGGGTTTACTAAAAAAAAGTTGGAAAAAGAACACCAAGTGGAACACAGCAAATCTAGGAAAGTCAAAAGTTCCATCCCATATTTTCCAAGCTGGGGTGTCCACCTCATTTATCCCATTACTTTTTTTGCTCTCCCTTGAAATCTGTGTCCACACAAAACTTGAGAAATATTGTCTTTTGCTTCGCTATCAAAGACTCGAGATATATGGACACTGTCTGTAACATCAAGGATGTCCAAAATTCCTTGCAAAAGGTCGGTAAAAATGTACATGTCCAGCATCTTTCATCGCTCTTGTACTTGGTATACGTATAACAGATTTAGTTGAATAAGAATTGATTGGAGCTGCCTCTCTTGTTTAATTTGTTTTTAATTTTATAATGTGAGCATTCAAGTCTTGTCTTCCAGCTTTGTGGGTTCTGAACTCTGAGAAATTTGATTGGCAGTTTTGAAGTGTTGTTTCTCTTTGTTCAGAGATTCAAGTCTTGTCTTGCAGTTTAGTGGCTTAGTTCTTTTTATGAGCCTATCTCTGGGGTTTGAGTTTTCACAGAATCGTGCTTCTTGGAAGAACTTAGCTTTATCAAATCATATCCATCTGATAGCTGAAGGCATCACTGTTGGTGCAGTTATATTTTGGTTATGGTCAAAAGGAAACTATGAACTCAATCTGCGCTTTATGGGCCATTGGCGAAGTAAAGAAGAGAGGAGAGAATAAGAGGAAAAGGAAAGACACTAAATTTTATTATTAAGATTTAGAGTGTATAGTCTATTAGGTGTATTTGTGATTTGATTAGGCTGATAATTGGGTTTATGATTCTGTAAATGGTTGAGTGAGCATCTCCAAGTAGTCAATATATTCACATTCTTAATGTATGCTGGAAGCTTCAATCCTGAGGCTCAGTAGGTCATATCCTCGACGCTTGTTTACCATTTTTGCATTGTATGTTCTTTTCGCACTACTGCAAAAGTGAGAACCTGGTTCTTTTCCCACATTATCCATGCTCACCAACAGAATTGGAATTAGAAGATTATCTGTATACACATGTAAAACATTAGCTAAACATTTTGGATATGTTCTTACCGTATGAAATAAATGCACAAATCTGCAATTTTTGTCATGCGCTAATAGCTTTTCGACTCATATGAACCTAATCTTAATGTTGTAGGTGGTGGATCTCGTGAGACGAGCCATTCCCATTGCTTTGATGCCCGAAGACGACCCGACAAGGAGCGAGCTAAAGCAGCTGCAAGAAAAGAAGGAAGAAATCGATGTACTGGCACATAAGCAGGTGCGACGAATACTGTGGACCGGGCTAGGTTTAGCCATTCTGCAAGTTGGACTCTTCTTCAGGCTAACATTCTGGGAATTTTCATGGGACGTAATGGAACCAATTGCATTTTTCACCACAACAACTGGATTAGTTATAGGCTATGCTTATTTCCTGTTCACTTCAAGAGATCCCACCTACCAGGACTTGTTGAAGAGGCTTTTCCTCTCAAGGCAAAGGAAGCTGTTCAAGAAGCATTCTTTTGATGTTTGCAGATTCAAGGAGTTGCAGAAAAAATGTAAGTCGCCTCTCGACGCCACGGCAAATATTAAAAATCGGGTGGGCGTAGATCTGGACCTTGAGGATTGTTTGAGTAGAGATTAATTACTATCATCATATACCCTTCCTTTATTAGGACAGGTAAGGGGTTAACTATTATTAACATCCCTAATCTTTTCTTTCTTTCTGGGGGCTGCCATTCAATTTTGGAGTTTAGAGTTATAAAACTAGTAAAGAATACTTGCAATTGTTCAATCAAATATGGAATGGAATGCACTGAAAATAACCGTTGCATTACCAAACCTTCAGTGTCTTTGTTGCCCAGCTGCATTGCTCTTGTACTCATGAAGTGAATTTTGATCTTTGGACTTAAAAACACTTTCTATAAGAATATTACATTGCAATTGAGAACGAAATTTGGGCTTACTATTCAAACAA

mRNA sequence

TTAAAAAGGTAATTATATATATATTATTTTGGTGCAACTGCGAAATAAAAATAAAATAAAATCTCCATTTTTGGAAGCAGCAAAGCGTCTAACGCGGTCCAAAAACAAAATAAAACAACCAACACGAAAGAGCCGCGTAGGGTTCTCGACCAAGCCCTTCCACGCCATTGCCTTGCCATTGCCGTTTCCCTCGGGACCTTCAAATCAACGCCTTAAAGCGCCAAACAAACCAATATAAACATTGCCCACTCCTGTCACATTGCTTCCTTTATTACATTCACTCTCTCAAATCAAATCAATTTCTTCTTCCACTCTCTTTCAATGGACGTTATGGAAGATTGCAGAATCATCGATGGAAGAATTTTTGGATTCCACGTTTGAGGATTATTACTGGAGGTTTCTGTTTCTGTTTTTGTTTTTGTTTTCTTTTTCAGCTTTTGAAGACGGGATGATGTGGAGGAAATGGTGTTCTTGTGCTAGTTTATTGAGTCGGAGATTGTTGGTGAATGGAGTTCCGGGGAATTCTTCTCCGGTACTTTCTTATTATGGAACTTTAGGGCTTGGTGGAGTTTCTTTGATTCCATCGACGGCGGTACGGTCATCGACCTGGTTTTGTACCAGAGTGGCTTCGTTTTCGTCGTCTGTGGATTCAGGAAATTCTACAGGAGTTCTTCGCGATGTGGGAAAAAGTGAGAGTGGTGGCCCTGGTCCTGGCCTTGGCCTTGGGTCTGTGAATGAAGAATCTGGTGATGGAATTGGTGGAGAGACGATTTCGTTTGGGGAAGCTAAAAGGCTGATGAGATTGGTTAATGTTGAGGCGCTGAAGATGAAATTGGGGACTGATGGGAAGGAGGCGATTGGATATTCGGAGCTTCTTGAAGCTTGTAAAAGCATGGGCGTTGCTAGGTCTCAGGATGAAGCTGCTGCTTTTGCTCGAGTTCTTGATGAAGCTGGTGTGGTTCTTCTTTTTCGCGATAAAGTCTATCTTCATCCGGACAAGGTGGTGGATCTCGTGAGACGAGCCATTCCCATTGCTTTGATGCCCGAAGACGACCCGACAAGGAGCGAGCTAAAGCAGCTGCAAGAAAAGAAGGAAGAAATCGATGTACTGGCACATAAGCAGGTGCGACGAATACTGTGGACCGGGCTAGGTTTAGCCATTCTGCAAGTTGGACTCTTCTTCAGGCTAACATTCTGGGAATTTTCATGGGACGTAATGGAACCAATTGCATTTTTCACCACAACAACTGGATTAGTTATAGGCTATGCTTATTTCCTGTTCACTTCAAGAGATCCCACCTACCAGGACTTGTTGAAGAGGCTTTTCCTCTCAAGGCAAAGGAAGCTGTTCAAGAAGCATTCTTTTGATGTTTGCAGATTCAAGGAGTTGCAGAAAAAATGTAAGTCGCCTCTCGACGCCACGGCAAATATTAAAAATCGGGTGGGCGTAGATCTGGACCTTGAGGATTGTTTGAGTAGAGATTAATTACTATCATCATATACCCTTCCTTTATTAGGACAGGTAAGGGGTTAACTATTATTAACATCCCTAATCTTTTCTTTCTTTCTGGGGGCTGCCATTCAATTTTGGAGTTTAGAGTTATAAAACTAGTAAAGAATACTTGCAATTGTTCAATCAAATATGGAATGGAATGCACTGAAAATAACCGTTGCATTACCAAACCTTCAGTGTCTTTGTTGCCCAGCTGCATTGCTCTTGTACTCATGAAGTGAATTTTGATCTTTGGACTTAAAAACACTTTCTATAAGAATATTACATTGCAATTGAGAACGAAATTTGGGCTTACTATTCAAACAA

Coding sequence (CDS)

ATGGAAGAATTTTTGGATTCCACGTTTGAGGATTATTACTGGAGGTTTCTGTTTCTGTTTTTGTTTTTGTTTTCTTTTTCAGCTTTTGAAGACGGGATGATGTGGAGGAAATGGTGTTCTTGTGCTAGTTTATTGAGTCGGAGATTGTTGGTGAATGGAGTTCCGGGGAATTCTTCTCCGGTACTTTCTTATTATGGAACTTTAGGGCTTGGTGGAGTTTCTTTGATTCCATCGACGGCGGTACGGTCATCGACCTGGTTTTGTACCAGAGTGGCTTCGTTTTCGTCGTCTGTGGATTCAGGAAATTCTACAGGAGTTCTTCGCGATGTGGGAAAAAGTGAGAGTGGTGGCCCTGGTCCTGGCCTTGGCCTTGGGTCTGTGAATGAAGAATCTGGTGATGGAATTGGTGGAGAGACGATTTCGTTTGGGGAAGCTAAAAGGCTGATGAGATTGGTTAATGTTGAGGCGCTGAAGATGAAATTGGGGACTGATGGGAAGGAGGCGATTGGATATTCGGAGCTTCTTGAAGCTTGTAAAAGCATGGGCGTTGCTAGGTCTCAGGATGAAGCTGCTGCTTTTGCTCGAGTTCTTGATGAAGCTGGTGTGGTTCTTCTTTTTCGCGATAAAGTCTATCTTCATCCGGACAAGGTGGTGGATCTCGTGAGACGAGCCATTCCCATTGCTTTGATGCCCGAAGACGACCCGACAAGGAGCGAGCTAAAGCAGCTGCAAGAAAAGAAGGAAGAAATCGATGTACTGGCACATAAGCAGGTGCGACGAATACTGTGGACCGGGCTAGGTTTAGCCATTCTGCAAGTTGGACTCTTCTTCAGGCTAACATTCTGGGAATTTTCATGGGACGTAATGGAACCAATTGCATTTTTCACCACAACAACTGGATTAGTTATAGGCTATGCTTATTTCCTGTTCACTTCAAGAGATCCCACCTACCAGGACTTGTTGAAGAGGCTTTTCCTCTCAAGGCAAAGGAAGCTGTTCAAGAAGCATTCTTTTGATGTTTGCAGATTCAAGGAGTTGCAGAAAAAATGTAAGTCGCCTCTCGACGCCACGGCAAATATTAAAAATCGGGTGGGCGTAGATCTGGACCTTGAGGATTGTTTGAGTAGAGATTAA

Protein sequence

MEEFLDSTFEDYYWRFLFLFLFLFSFSAFEDGMMWRKWCSCASLLSRRLLVNGVPGNSSPVLSYYGTLGLGGVSLIPSTAVRSSTWFCTRVASFSSSVDSGNSTGVLRDVGKSESGGPGPGLGLGSVNEESGDGIGGETISFGEAKRLMRLVNVEALKMKLGTDGKEAIGYSELLEACKSMGVARSQDEAAAFARVLDEAGVVLLFRDKVYLHPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDVLAHKQVRRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQDLLKRLFLSRQRKLFKKHSFDVCRFKELQKKCKSPLDATANIKNRVGVDLDLEDCLSRD
Homology
BLAST of Lsi10G014940 vs. ExPASy Swiss-Prot
Match: F4I111 (Calcium uniporter protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g09575 PE=2 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 2.7e-83
Identity = 157/238 (65.97%), Postives = 190/238 (79.83%), Query Frame = 0

Query: 140 ISFGEAKRLMRLVNVEALKMKL-GTDGKEAIGYSELLEACKSMGVARSQDEAAAFARVLD 199
           I+  EAK+LMRLVNVE +K KL G   ++ + YS LLEA + MG+ARS DEA  FARVLD
Sbjct: 50  ITIAEAKKLMRLVNVEDMKKKLVGVADRDVVSYSTLLEASQGMGIARSPDEAHVFARVLD 109

Query: 200 EAGVVLLFRDKVYLHPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDVLAHKQV 259
           +AGVVL+FRDKVYLHPDKVVDL+RRA+P+   PE+D  + E  +L+  KEEIDVLAH+QV
Sbjct: 110 DAGVVLIFRDKVYLHPDKVVDLIRRAMPLDQNPEEDQIKEEFNKLRIMKEEIDVLAHRQV 169

Query: 260 RRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQ 319
           R+ILW GL  +++Q+GLFFRLTFWEFSWDVMEPI FF T TG+++GYAYFL TSRDPTYQ
Sbjct: 170 RKILWCGLATSMVQIGLFFRLTFWEFSWDVMEPITFFATATGIIVGYAYFLMTSRDPTYQ 229

Query: 320 DLLKRLFLSRQRKLFKKHSFDVCRFKELQK--KCKSPLDATANIKNRVGVDLDLEDCL 375
           D +KRLFLSRQRKL K H FD  RFKEL++  K  S   A A+I+NRVG++LDLED L
Sbjct: 230 DFMKRLFLSRQRKLLKSHKFDCERFKELERLFKMTSSCHAAASIRNRVGLELDLEDAL 287

BLAST of Lsi10G014940 vs. ExPASy Swiss-Prot
Match: Q8VYR0 (Calcium uniporter protein 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g57610 PE=2 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 7.8e-83
Identity = 160/259 (61.78%), Postives = 199/259 (76.83%), Query Frame = 0

Query: 123 GLGSVNEESGDGIGGETISFGEAKRLMRLVNVEALKMKL-GTDGKEAIGYSELLEACKSM 182
           G   +N ES +    + ++  EAK+LMRLVNVE +K KL G   KE + Y+ L+EA + +
Sbjct: 29  GFRCLNVESKEEDEKKDMTVLEAKKLMRLVNVEDMKKKLIGMGDKEMVTYTTLIEASQGL 88

Query: 183 GVARSQDEAAAFARVLDEAGVVLLFRDKVYLHPDKVVDLVRRAIPIALMPEDDPTRSELK 242
           G+ARS DEA AFARVLD+AGV+L+FRDKVYLHP KVVDL+R+A+P+ L P+D+  R E  
Sbjct: 89  GIARSLDEAHAFARVLDDAGVILIFRDKVYLHPHKVVDLIRKAVPLGLNPDDELIREEFD 148

Query: 243 QLQEKKEEIDVLAHKQVRRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTTGL 302
           +++  KEEIDVLAH+QVR+ILW GLG +++Q+G+F RLTFWEFSWDVMEPI FFTT TG+
Sbjct: 149 KMRSMKEEIDVLAHQQVRKILWGGLGYSVVQIGIFVRLTFWEFSWDVMEPITFFTTATGI 208

Query: 303 VIGYAYFLFTSRDPTYQDLLKRLFLSRQRKLFKKHSFDVCRFKELQKKCK------SPLD 362
           ++GYAYFL TSRDPTYQD +KRLFLSRQRKL K H FD  RFKEL+ K K      S   
Sbjct: 209 IVGYAYFLMTSRDPTYQDFMKRLFLSRQRKLLKSHKFDAERFKELENKWKITSCSSSSCH 268

Query: 363 ATANIKNRVGVDLDLEDCL 375
           A A+I+NRVGVDLDLED L
Sbjct: 269 ANASIRNRVGVDLDLEDSL 287

BLAST of Lsi10G014940 vs. ExPASy Swiss-Prot
Match: O64823 (Calcium uniporter protein 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g23790 PE=2 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 2.2e-37
Identity = 87/225 (38.67%), Postives = 134/225 (59.56%), Query Frame = 0

Query: 139 TISFGEAKRLMRLVNVEALKMKLGTDGKEAIGYSELLEACKSMGVARSQ-DEAAAFARVL 198
           +++  + K+L+R   +E +K KL   G+  + Y E +  C   G A S  D  +  A++L
Sbjct: 109 SLTVADTKKLLRAAQIEIVKSKLRETGRSWMPYKEFVSVC---GEASSDPDLGSKIAKML 168

Query: 199 DEAGVVLLFRDKVYLHPDKVVDLVRRAIPI-ALMPEDDPTRSELKQLQEKKEEIDVLAHK 258
           D++  V++  D V + PD+V   +   +P+  +   +DP R E K+L+ +K  IDV AH 
Sbjct: 169 DDSANVIVLGDSVCIRPDQVTKSIEGLLPLPKIHNPNDPRRIEFKELEAEKAVIDVKAHT 228

Query: 259 QVRRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPT 318
            VR+ LW GLG  ILQ   F RLTFWE SWDVMEPI F+ T+   + GYA+FL TS++P+
Sbjct: 229 LVRKELWAGLGYLILQTAGFMRLTFWELSWDVMEPICFYVTSVYFMAGYAFFLRTSKEPS 288

Query: 319 YQDLLKRLFLSRQRKLFKKHSFDVCRFKELQKK-CKSPLDATANI 361
           ++   +  F ++QRKL  ++ FD+ R+ EL+K  C  P D  + I
Sbjct: 289 FEGFYQSRFEAKQRKLMNEYEFDLERYNELKKLFCSKPSDHVSKI 330

BLAST of Lsi10G014940 vs. ExPASy Swiss-Prot
Match: Q1PE15 (Calcium uniporter protein 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g36820 PE=2 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 3.6e-35
Identity = 82/206 (39.81%), Postives = 123/206 (59.71%), Query Frame = 0

Query: 144 EAKRLMRLVNVEALKMKLGTDGKEAIGYSELLEACKSMGVARSQDEAAAFARVLDEAGVV 203
           + K+L+R   +E +K KL   GK  I YS+ L  C    +  SQ   A  A++LD++G V
Sbjct: 116 DVKKLLRAAEIEVIKTKLMETGKIWIRYSDFLGVCSDSSLDPSQ--GALIAKMLDDSGNV 175

Query: 204 LLFRDKVYLHPDKVVDLVRRAIPIA-LMPEDDPTRSELKQLQEKKEEIDVLAHKQVRRIL 263
           ++  + V L P ++   +   +P++ +   +DP R EL +L+  K  ID  AH  VRR L
Sbjct: 176 IVMGNSVCLRPHQLTKSIEGLLPLSQIHNPNDPRRKELNELEAIKTVIDQKAHSLVRREL 235

Query: 264 WTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQDLLK 323
           W GLG  I+Q   F RLTFW+ +WDVMEPI F+ ++   + GY +FL TSR+P++Q   +
Sbjct: 236 WAGLGYLIIQTAGFMRLTFWDLTWDVMEPICFYVSSVYFMAGYTFFLKTSREPSFQGFYQ 295

Query: 324 RLFLSRQRKLFKKHSFDVCRFKELQK 349
             F ++QRKL +   FDV R+ EL+K
Sbjct: 296 SRFEAKQRKLMQSEDFDVGRYDELKK 319

BLAST of Lsi10G014940 vs. ExPASy Swiss-Prot
Match: Q9LVR5 (Calcium uniporter protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g66650 PE=2 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 1.0e-34
Identity = 84/209 (40.19%), Postives = 122/209 (58.37%), Query Frame = 0

Query: 140 ISFGEAKRLMRLVNVEALKMKLGTDGKEAIGYSELLEACKSMGVARSQDEAAAFARVLDE 199
           ++  + K+LMR   +E +K KL   GK  + YSE +  C     +   ++    A +LDE
Sbjct: 103 VTVEDVKKLMRAAEMELVKSKLREIGKNWVPYSEFVRVCGE--YSSDPEQGNRVANMLDE 162

Query: 200 AGVVLLFRDKVYLHPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDVLAHKQVR 259
           AG V++    V L P+++   +   IP      D  TR E +QL+  K +ID  A   VR
Sbjct: 163 AGNVIVLGKLVCLKPEELTSAMAGLIPTLEPSLDAETRQEFEQLEIIKSDIDKRADDLVR 222

Query: 260 RILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQD 319
           + LW GLGL + Q   FFRLTFWE SWDVMEPI F+ T+T  + GYA+FL TS++P+++ 
Sbjct: 223 KELWAGLGLIMAQTVGFFRLTFWELSWDVMEPICFYVTSTYFMAGYAFFLRTSKEPSFEG 282

Query: 320 LLKRLFLSRQRKLFKKHSFDVCRFKELQK 349
             K  F ++Q++L K   FD+ RF +LQK
Sbjct: 283 FYKSRFETKQKRLIKMLDFDIDRFTKLQK 309

BLAST of Lsi10G014940 vs. ExPASy TrEMBL
Match: A0A6J1FV50 (Calcium uniporter protein OS=Cucurbita moschata OX=3662 GN=LOC111448718 PE=3 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 1.5e-166
Identity = 313/345 (90.72%), Postives = 319/345 (92.46%), Query Frame = 0

Query: 33  MMWRKWCSCASLLSRRLLVNGVPGNSSPVLSYYGTLGLGGVSLIPSTAVRSSTWFCTRVA 92
           MMWR+WCS A LLSRRLLVNGV   SSPV S YG  GLGGVSLIPSTAVRSS WFC  V 
Sbjct: 1   MMWRRWCSSACLLSRRLLVNGVRRPSSPV-SSYGDFGLGGVSLIPSTAVRSSAWFCNGVV 60

Query: 93  SFSSSVDSGNSTGVLRDVGKSESGGPGPGLGLGSVNEESGDGIGGETISFGEAKRLMRLV 152
           SFSSSVDS  S GV  D GK  SGGPG GLGLGSVNEESG+ I GETISF EAKRLMRLV
Sbjct: 61  SFSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGNEISGETISFAEAKRLMRLV 120

Query: 153 NVEALKMKLGTDGKEAIGYSELLEACKSMGVARSQDEAAAFARVLDEAGVVLLFRDKVYL 212
           NVEALKMKLGTDGKEAIGYSEL+EACKSMG+ARSQDEAAAFARVLDEAGVVLLFRDKVYL
Sbjct: 121 NVEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYL 180

Query: 213 HPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDVLAHKQVRRILWTGLGLAILQ 272
           HPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEID+LAHKQVRRILWTGLGLAILQ
Sbjct: 181 HPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQ 240

Query: 273 VGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQDLLKRLFLSRQRKL 332
           VGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFL TSRDPTYQDLLKRLFLSRQRKL
Sbjct: 241 VGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKL 300

Query: 333 FKKHSFDVCRFKELQKKCKSPLDATANIKNRVGVDLDLEDCLSRD 378
           FKKHSFDVCRFKELQKKCK+PLDATANIKNRVGVDLDLEDCLSRD
Sbjct: 301 FKKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD 344

BLAST of Lsi10G014940 vs. ExPASy TrEMBL
Match: A0A6J1JHV8 (Calcium uniporter protein OS=Cucurbita maxima OX=3661 GN=LOC111484499 PE=3 SV=1)

HSP 1 Score: 592.0 bits (1525), Expect = 1.7e-165
Identity = 311/345 (90.14%), Postives = 318/345 (92.17%), Query Frame = 0

Query: 33  MMWRKWCSCASLLSRRLLVNGVPGNSSPVLSYYGTLGLGGVSLIPSTAVRSSTWFCTRVA 92
           MMWR+WCS  SLLSR LLVNGV   SSPV S YG  GLGGVSLI STAVRSS WFCTRV 
Sbjct: 1   MMWRRWCSSVSLLSRSLLVNGVRRPSSPV-SSYGAFGLGGVSLISSTAVRSSAWFCTRVV 60

Query: 93  SFSSSVDSGNSTGVLRDVGKSESGGPGPGLGLGSVNEESGDGIGGETISFGEAKRLMRLV 152
           SFSSSVDS  S GV  D GK  SGGPG GLGLGSVNEESG+GI GETISF EAKRLMRLV
Sbjct: 61  SFSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGNGISGETISFAEAKRLMRLV 120

Query: 153 NVEALKMKLGTDGKEAIGYSELLEACKSMGVARSQDEAAAFARVLDEAGVVLLFRDKVYL 212
           NVEALKMKLGTDGKEAIGY EL+EACKSMG+ARSQDEAAAFARVLDEAGVVLLFRDKVYL
Sbjct: 121 NVEALKMKLGTDGKEAIGYLELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYL 180

Query: 213 HPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDVLAHKQVRRILWTGLGLAILQ 272
           HPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEID+LAHKQVRRILWTGLGLAILQ
Sbjct: 181 HPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQ 240

Query: 273 VGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQDLLKRLFLSRQRKL 332
           V LFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFL TSRDPTYQDLLKRLFLSRQRKL
Sbjct: 241 VALFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKL 300

Query: 333 FKKHSFDVCRFKELQKKCKSPLDATANIKNRVGVDLDLEDCLSRD 378
           FKKHSFDVCRFKELQKKCK+PL+ATANIKNRVGVDLDLEDCLSRD
Sbjct: 301 FKKHSFDVCRFKELQKKCKTPLNATANIKNRVGVDLDLEDCLSRD 344

BLAST of Lsi10G014940 vs. ExPASy TrEMBL
Match: A0A5A7SU81 (Calcium uniporter protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold104G00670 PE=3 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 2.5e-161
Identity = 310/346 (89.60%), Postives = 319/346 (92.20%), Query Frame = 0

Query: 34  MWRKWCSCA-SLLSRRLLVNGVPGNSSPVLSYYGTLGL-GGVSLIPSTAVRSSTWFCTRV 93
           MWRK CS A SL +RRLLVNGV G+SSPV  YYGTLGL  GVS  PS  +RSS+WFCT V
Sbjct: 1   MWRKCCSSATSLFTRRLLVNGVSGSSSPV--YYGTLGLSAGVSFNPSPPLRSSSWFCTGV 60

Query: 94  ASFSSSVDSGNSTGVLRDVGKSESGGPGPGLGLGSVNEESGDGIGGETISFGEAKRLMRL 153
           A FSSSVDSGN TGV RDV KSESGG   G G+GSV EESG+GIG E ISFGEAKRLMRL
Sbjct: 61  ALFSSSVDSGNFTGVRRDVDKSESGG---GPGIGSVKEESGNGIGEEEISFGEAKRLMRL 120

Query: 154 VNVEALKMKLGTDGKEAIGYSELLEACKSMGVARSQDEAAAFARVLDEAGVVLLFRDKVY 213
           VNVEALKMKLGTDGKEAIGY ELLEACKSMGVARS+DEAA FARVLDEAGVVLLFRDKVY
Sbjct: 121 VNVEALKMKLGTDGKEAIGYLELLEACKSMGVARSEDEAATFARVLDEAGVVLLFRDKVY 180

Query: 214 LHPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDVLAHKQVRRILWTGLGLAIL 273
           LHPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDVLAHKQVRRILWTGLGLA+L
Sbjct: 181 LHPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDVLAHKQVRRILWTGLGLAVL 240

Query: 274 QVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQDLLKRLFLSRQRK 333
           QVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQDLLKRLFLSRQRK
Sbjct: 241 QVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQDLLKRLFLSRQRK 300

Query: 334 LFKKHSFDVCRFKELQKKCKSPLDATANIKNRVGVDLDLEDCLSRD 378
           LFKKHSFDVCRFKELQKKCKSPLDATANIKNRVGVDL+LEDCLSRD
Sbjct: 301 LFKKHSFDVCRFKELQKKCKSPLDATANIKNRVGVDLELEDCLSRD 341

BLAST of Lsi10G014940 vs. ExPASy TrEMBL
Match: A0A1S3C2X1 (Calcium uniporter protein OS=Cucumis melo OX=3656 GN=LOC103496260 PE=3 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 2.5e-161
Identity = 310/346 (89.60%), Postives = 319/346 (92.20%), Query Frame = 0

Query: 34  MWRKWCSCA-SLLSRRLLVNGVPGNSSPVLSYYGTLGL-GGVSLIPSTAVRSSTWFCTRV 93
           MWRK CS A SL +RRLLVNGV G+SSPV  YYGTLGL  GVS  PS  +RSS+WFCT V
Sbjct: 1   MWRKCCSSATSLFTRRLLVNGVSGSSSPV--YYGTLGLSAGVSFNPSPPLRSSSWFCTGV 60

Query: 94  ASFSSSVDSGNSTGVLRDVGKSESGGPGPGLGLGSVNEESGDGIGGETISFGEAKRLMRL 153
           A FSSSVDSGN TGV RDV KSESGG   G G+GSV EESG+GIG E ISFGEAKRLMRL
Sbjct: 61  ALFSSSVDSGNFTGVRRDVDKSESGG---GPGIGSVKEESGNGIGEEEISFGEAKRLMRL 120

Query: 154 VNVEALKMKLGTDGKEAIGYSELLEACKSMGVARSQDEAAAFARVLDEAGVVLLFRDKVY 213
           VNVEALKMKLGTDGKEAIGY ELLEACKSMGVARS+DEAA FARVLDEAGVVLLFRDKVY
Sbjct: 121 VNVEALKMKLGTDGKEAIGYLELLEACKSMGVARSEDEAATFARVLDEAGVVLLFRDKVY 180

Query: 214 LHPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDVLAHKQVRRILWTGLGLAIL 273
           LHPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDVLAHKQVRRILWTGLGLA+L
Sbjct: 181 LHPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDVLAHKQVRRILWTGLGLAVL 240

Query: 274 QVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQDLLKRLFLSRQRK 333
           QVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQDLLKRLFLSRQRK
Sbjct: 241 QVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQDLLKRLFLSRQRK 300

Query: 334 LFKKHSFDVCRFKELQKKCKSPLDATANIKNRVGVDLDLEDCLSRD 378
           LFKKHSFDVCRFKELQKKCKSPLDATANIKNRVGVDL+LEDCLSRD
Sbjct: 301 LFKKHSFDVCRFKELQKKCKSPLDATANIKNRVGVDLELEDCLSRD 341

BLAST of Lsi10G014940 vs. ExPASy TrEMBL
Match: A0A0A0KD48 (Calcium uniporter protein OS=Cucumis sativus OX=3659 GN=Csa_6G133830 PE=3 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 1.1e-159
Identity = 306/346 (88.44%), Postives = 317/346 (91.62%), Query Frame = 0

Query: 34  MWRKWCSCA-SLLSRRLLVNGVPGNSSPVLSYYGTLGL-GGVSLIPSTAVRSSTWFCTRV 93
           MWRK CS A SL  RRL VNG+ G+SSPV  YYGTLGL  GVS IPS  VRS+ WFCT +
Sbjct: 1   MWRKCCSSATSLFIRRLFVNGLSGSSSPV--YYGTLGLSAGVSFIPSPPVRSNGWFCTGL 60

Query: 94  ASFSSSVDSGNSTGVLRDVGKSESGGPGPGLGLGSVNEESGDGIGGETISFGEAKRLMRL 153
           A FSSSVDSGN TGV RDV KSESGG   G G+GSVN ESG+GI GE ISFGEAKRLMRL
Sbjct: 61  ALFSSSVDSGNFTGVRRDVDKSESGG---GPGIGSVNGESGNGITGEAISFGEAKRLMRL 120

Query: 154 VNVEALKMKLGTDGKEAIGYSELLEACKSMGVARSQDEAAAFARVLDEAGVVLLFRDKVY 213
           VNVEALK KLGTDGKEAIGY EL+EACKSMGVARS+DEAAAFARVLDEAGVVLLFRDKVY
Sbjct: 121 VNVEALKTKLGTDGKEAIGYLELIEACKSMGVARSEDEAAAFARVLDEAGVVLLFRDKVY 180

Query: 214 LHPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDVLAHKQVRRILWTGLGLAIL 273
           LHPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDVLAHKQVRRILWTGLGLA+L
Sbjct: 181 LHPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDVLAHKQVRRILWTGLGLAVL 240

Query: 274 QVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQDLLKRLFLSRQRK 333
           QVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQDLLKRLFLSRQRK
Sbjct: 241 QVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQDLLKRLFLSRQRK 300

Query: 334 LFKKHSFDVCRFKELQKKCKSPLDATANIKNRVGVDLDLEDCLSRD 378
           LFKKHSFDVCRFKELQKKCKSPLDATANIKNRVG+DL+LEDCLSRD
Sbjct: 301 LFKKHSFDVCRFKELQKKCKSPLDATANIKNRVGIDLELEDCLSRD 341

BLAST of Lsi10G014940 vs. NCBI nr
Match: XP_038902671.1 (calcium uniporter protein 5, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 642.9 bits (1657), Expect = 1.7e-180
Identity = 345/378 (91.27%), Postives = 351/378 (92.86%), Query Frame = 0

Query: 1   MEEFLDSTFEDYYWRFLFLFLFLFSFSAFEDGMMWRKWCSCASLLSRRLLVNGVPGNSSP 60
           MEE LDSTF     RFLFLF   F FSA E+GMMWRKWCS  SLLSRRLLVNGVPGNS  
Sbjct: 1   MEESLDSTFSACCRRFLFLF---FVFSALENGMMWRKWCSSVSLLSRRLLVNGVPGNS-- 60

Query: 61  VLSYYGTLGLG-GVSLIPSTAVRSSTWFCTRVASFSSSVDSGNSTGVLRDVGKSESGGPG 120
             SYYGTLGLG GVSL PS AVRSSTWFCTRVASFSSSVDSGNSTG+ RD  KSESGGP 
Sbjct: 61  --SYYGTLGLGRGVSLNPSPAVRSSTWFCTRVASFSSSVDSGNSTGIRRDAEKSESGGP- 120

Query: 121 PGLGLGSVNEESGDGIGGETISFGEAKRLMRLVNVEALKMKLGTDGKEAIGYSELLEACK 180
              GLGSVNEESG+GIGGETISFGEAKRLMRLVNVEALKMKLGTDGKEAIGY+ELLEACK
Sbjct: 121 ---GLGSVNEESGNGIGGETISFGEAKRLMRLVNVEALKMKLGTDGKEAIGYTELLEACK 180

Query: 181 SMGVARSQDEAAAFARVLDEAGVVLLFRDKVYLHPDKVVDLVRRAIPIALMPEDDPTRSE 240
           SMGVARSQDEAAAFARVLDEAGV+LLFRDKVYLHPDKVVDLVRRAIPIALMPEDDPTRSE
Sbjct: 181 SMGVARSQDEAAAFARVLDEAGVILLFRDKVYLHPDKVVDLVRRAIPIALMPEDDPTRSE 240

Query: 241 LKQLQEKKEEIDVLAHKQVRRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTT 300
           LKQLQEKKEEIDVLAHKQVRRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTT
Sbjct: 241 LKQLQEKKEEIDVLAHKQVRRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTT 300

Query: 301 GLVIGYAYFLFTSRDPTYQDLLKRLFLSRQRKLFKKHSFDVCRFKELQKKCKSPLDATAN 360
           GLVIGYAYFLFTSRDPTYQDLLKRLFLSRQRKLFKKHSFDVCRFKELQKKCKSPLDATAN
Sbjct: 301 GLVIGYAYFLFTSRDPTYQDLLKRLFLSRQRKLFKKHSFDVCRFKELQKKCKSPLDATAN 360

Query: 361 IKNRVGVDLDLEDCLSRD 378
           IKNRVGVDL+LEDCLSRD
Sbjct: 361 IKNRVGVDLELEDCLSRD 367

BLAST of Lsi10G014940 vs. NCBI nr
Match: KAG6570611.1 (Calcium uniporter protein 5, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 597.0 bits (1538), Expect = 1.1e-166
Identity = 314/345 (91.01%), Postives = 319/345 (92.46%), Query Frame = 0

Query: 33  MMWRKWCSCASLLSRRLLVNGVPGNSSPVLSYYGTLGLGGVSLIPSTAVRSSTWFCTRVA 92
           MMWR+WCS A LLSRRLLVNGV   SSPV S YG  GLGGVSLIPSTAVRSS WFC  V 
Sbjct: 1   MMWRRWCSSACLLSRRLLVNGVRRPSSPV-SSYGDFGLGGVSLIPSTAVRSSAWFCNGVV 60

Query: 93  SFSSSVDSGNSTGVLRDVGKSESGGPGPGLGLGSVNEESGDGIGGETISFGEAKRLMRLV 152
           SFSSSVDS  S GV  D GK  SGGPG GLGLGSVNEESG GI GETISF EAKRLMRLV
Sbjct: 61  SFSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGYGISGETISFAEAKRLMRLV 120

Query: 153 NVEALKMKLGTDGKEAIGYSELLEACKSMGVARSQDEAAAFARVLDEAGVVLLFRDKVYL 212
           NVEALKMKLGTDGKEAIGYSEL+EACKSMG+ARSQDEAAAFARVLDEAGVVLLFRDKVYL
Sbjct: 121 NVEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYL 180

Query: 213 HPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDVLAHKQVRRILWTGLGLAILQ 272
           HPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEID+LAHKQVRRILWTGLGLAILQ
Sbjct: 181 HPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQ 240

Query: 273 VGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQDLLKRLFLSRQRKL 332
           VGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFL TSRDPTYQDLLKRLFLSRQRKL
Sbjct: 241 VGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKL 300

Query: 333 FKKHSFDVCRFKELQKKCKSPLDATANIKNRVGVDLDLEDCLSRD 378
           FKKHSFDVCRFKELQKKCK+PLDATANIKNRVGVDLDLEDCLSRD
Sbjct: 301 FKKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD 344

BLAST of Lsi10G014940 vs. NCBI nr
Match: XP_022944197.1 (calcium uniporter protein 5, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 595.5 bits (1534), Expect = 3.2e-166
Identity = 313/345 (90.72%), Postives = 319/345 (92.46%), Query Frame = 0

Query: 33  MMWRKWCSCASLLSRRLLVNGVPGNSSPVLSYYGTLGLGGVSLIPSTAVRSSTWFCTRVA 92
           MMWR+WCS A LLSRRLLVNGV   SSPV S YG  GLGGVSLIPSTAVRSS WFC  V 
Sbjct: 1   MMWRRWCSSACLLSRRLLVNGVRRPSSPV-SSYGDFGLGGVSLIPSTAVRSSAWFCNGVV 60

Query: 93  SFSSSVDSGNSTGVLRDVGKSESGGPGPGLGLGSVNEESGDGIGGETISFGEAKRLMRLV 152
           SFSSSVDS  S GV  D GK  SGGPG GLGLGSVNEESG+ I GETISF EAKRLMRLV
Sbjct: 61  SFSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGNEISGETISFAEAKRLMRLV 120

Query: 153 NVEALKMKLGTDGKEAIGYSELLEACKSMGVARSQDEAAAFARVLDEAGVVLLFRDKVYL 212
           NVEALKMKLGTDGKEAIGYSEL+EACKSMG+ARSQDEAAAFARVLDEAGVVLLFRDKVYL
Sbjct: 121 NVEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYL 180

Query: 213 HPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDVLAHKQVRRILWTGLGLAILQ 272
           HPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEID+LAHKQVRRILWTGLGLAILQ
Sbjct: 181 HPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQ 240

Query: 273 VGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQDLLKRLFLSRQRKL 332
           VGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFL TSRDPTYQDLLKRLFLSRQRKL
Sbjct: 241 VGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKL 300

Query: 333 FKKHSFDVCRFKELQKKCKSPLDATANIKNRVGVDLDLEDCLSRD 378
           FKKHSFDVCRFKELQKKCK+PLDATANIKNRVGVDLDLEDCLSRD
Sbjct: 301 FKKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD 344

BLAST of Lsi10G014940 vs. NCBI nr
Match: XP_023512709.1 (calcium uniporter protein 5, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 593.2 bits (1528), Expect = 1.6e-165
Identity = 314/347 (90.49%), Postives = 320/347 (92.22%), Query Frame = 0

Query: 33  MMWRKWCSCASLLSRRLLVNGVPGNSSPVLSYYGTLGLGGVSLIPSTAVRSSTWFCTRVA 92
           MMWR+WCS A LLSRRLLVNGV   SSPV S YG  GLGGVSLIPSTAVRSS WFCTRV 
Sbjct: 1   MMWRRWCSSACLLSRRLLVNGVRRPSSPV-SSYGDFGLGGVSLIPSTAVRSSAWFCTRVV 60

Query: 93  SFSSSVDSGNSTGVLRDVGKSESGGP--GPGLGLGSVNEESGDGIGGETISFGEAKRLMR 152
           SFSSSVDS  S GV  D GK  SGGP  G GLGLGSVNEESG+ I GETISF EAKRLMR
Sbjct: 61  SFSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGLGSVNEESGNEISGETISFAEAKRLMR 120

Query: 153 LVNVEALKMKLGTDGKEAIGYSELLEACKSMGVARSQDEAAAFARVLDEAGVVLLFRDKV 212
           LVNVEALKMKLGTDGKEAIGYSEL+EACKSMG+ARSQDEAAAFARVLDEAGVVLLFRDKV
Sbjct: 121 LVNVEALKMKLGTDGKEAIGYSELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKV 180

Query: 213 YLHPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDVLAHKQVRRILWTGLGLAI 272
           YLHPDKVVDLVRRAIPIALMPEDDPTRSELK LQEKKEEID+LAHKQVRRILWTGLGLAI
Sbjct: 181 YLHPDKVVDLVRRAIPIALMPEDDPTRSELKHLQEKKEEIDILAHKQVRRILWTGLGLAI 240

Query: 273 LQVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQDLLKRLFLSRQR 332
           LQVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFL TSRDPTYQDLLKRLFLSRQR
Sbjct: 241 LQVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQR 300

Query: 333 KLFKKHSFDVCRFKELQKKCKSPLDATANIKNRVGVDLDLEDCLSRD 378
           KLFKKHSFDVCRFKELQKKCK+PLDATANIKNRVGVDLDLEDCLSRD
Sbjct: 301 KLFKKHSFDVCRFKELQKKCKTPLDATANIKNRVGVDLDLEDCLSRD 346

BLAST of Lsi10G014940 vs. NCBI nr
Match: XP_022986898.1 (calcium uniporter protein 5, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 592.0 bits (1525), Expect = 3.5e-165
Identity = 311/345 (90.14%), Postives = 318/345 (92.17%), Query Frame = 0

Query: 33  MMWRKWCSCASLLSRRLLVNGVPGNSSPVLSYYGTLGLGGVSLIPSTAVRSSTWFCTRVA 92
           MMWR+WCS  SLLSR LLVNGV   SSPV S YG  GLGGVSLI STAVRSS WFCTRV 
Sbjct: 1   MMWRRWCSSVSLLSRSLLVNGVRRPSSPV-SSYGAFGLGGVSLISSTAVRSSAWFCTRVV 60

Query: 93  SFSSSVDSGNSTGVLRDVGKSESGGPGPGLGLGSVNEESGDGIGGETISFGEAKRLMRLV 152
           SFSSSVDS  S GV  D GK  SGGPG GLGLGSVNEESG+GI GETISF EAKRLMRLV
Sbjct: 61  SFSSSVDSEKSAGVGSDGGKRASGGPGLGLGLGSVNEESGNGISGETISFAEAKRLMRLV 120

Query: 153 NVEALKMKLGTDGKEAIGYSELLEACKSMGVARSQDEAAAFARVLDEAGVVLLFRDKVYL 212
           NVEALKMKLGTDGKEAIGY EL+EACKSMG+ARSQDEAAAFARVLDEAGVVLLFRDKVYL
Sbjct: 121 NVEALKMKLGTDGKEAIGYLELIEACKSMGIARSQDEAAAFARVLDEAGVVLLFRDKVYL 180

Query: 213 HPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDVLAHKQVRRILWTGLGLAILQ 272
           HPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEID+LAHKQVRRILWTGLGLAILQ
Sbjct: 181 HPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDILAHKQVRRILWTGLGLAILQ 240

Query: 273 VGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQDLLKRLFLSRQRKL 332
           V LFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFL TSRDPTYQDLLKRLFLSRQRKL
Sbjct: 241 VALFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLITSRDPTYQDLLKRLFLSRQRKL 300

Query: 333 FKKHSFDVCRFKELQKKCKSPLDATANIKNRVGVDLDLEDCLSRD 378
           FKKHSFDVCRFKELQKKCK+PL+ATANIKNRVGVDLDLEDCLSRD
Sbjct: 301 FKKHSFDVCRFKELQKKCKTPLNATANIKNRVGVDLDLEDCLSRD 344

BLAST of Lsi10G014940 vs. TAIR 10
Match: AT1G09575.1 (Protein of unknown function (DUF607) )

HSP 1 Score: 310.5 bits (794), Expect = 1.9e-84
Identity = 157/238 (65.97%), Postives = 190/238 (79.83%), Query Frame = 0

Query: 140 ISFGEAKRLMRLVNVEALKMKL-GTDGKEAIGYSELLEACKSMGVARSQDEAAAFARVLD 199
           I+  EAK+LMRLVNVE +K KL G   ++ + YS LLEA + MG+ARS DEA  FARVLD
Sbjct: 50  ITIAEAKKLMRLVNVEDMKKKLVGVADRDVVSYSTLLEASQGMGIARSPDEAHVFARVLD 109

Query: 200 EAGVVLLFRDKVYLHPDKVVDLVRRAIPIALMPEDDPTRSELKQLQEKKEEIDVLAHKQV 259
           +AGVVL+FRDKVYLHPDKVVDL+RRA+P+   PE+D  + E  +L+  KEEIDVLAH+QV
Sbjct: 110 DAGVVLIFRDKVYLHPDKVVDLIRRAMPLDQNPEEDQIKEEFNKLRIMKEEIDVLAHRQV 169

Query: 260 RRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPTYQ 319
           R+ILW GL  +++Q+GLFFRLTFWEFSWDVMEPI FF T TG+++GYAYFL TSRDPTYQ
Sbjct: 170 RKILWCGLATSMVQIGLFFRLTFWEFSWDVMEPITFFATATGIIVGYAYFLMTSRDPTYQ 229

Query: 320 DLLKRLFLSRQRKLFKKHSFDVCRFKELQK--KCKSPLDATANIKNRVGVDLDLEDCL 375
           D +KRLFLSRQRKL K H FD  RFKEL++  K  S   A A+I+NRVG++LDLED L
Sbjct: 230 DFMKRLFLSRQRKLLKSHKFDCERFKELERLFKMTSSCHAAASIRNRVGLELDLEDAL 287

BLAST of Lsi10G014940 vs. TAIR 10
Match: AT1G57610.1 (Protein of unknown function (DUF607) )

HSP 1 Score: 308.9 bits (790), Expect = 5.5e-84
Identity = 160/259 (61.78%), Postives = 199/259 (76.83%), Query Frame = 0

Query: 123 GLGSVNEESGDGIGGETISFGEAKRLMRLVNVEALKMKL-GTDGKEAIGYSELLEACKSM 182
           G   +N ES +    + ++  EAK+LMRLVNVE +K KL G   KE + Y+ L+EA + +
Sbjct: 29  GFRCLNVESKEEDEKKDMTVLEAKKLMRLVNVEDMKKKLIGMGDKEMVTYTTLIEASQGL 88

Query: 183 GVARSQDEAAAFARVLDEAGVVLLFRDKVYLHPDKVVDLVRRAIPIALMPEDDPTRSELK 242
           G+ARS DEA AFARVLD+AGV+L+FRDKVYLHP KVVDL+R+A+P+ L P+D+  R E  
Sbjct: 89  GIARSLDEAHAFARVLDDAGVILIFRDKVYLHPHKVVDLIRKAVPLGLNPDDELIREEFD 148

Query: 243 QLQEKKEEIDVLAHKQVRRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTTGL 302
           +++  KEEIDVLAH+QVR+ILW GLG +++Q+G+F RLTFWEFSWDVMEPI FFTT TG+
Sbjct: 149 KMRSMKEEIDVLAHQQVRKILWGGLGYSVVQIGIFVRLTFWEFSWDVMEPITFFTTATGI 208

Query: 303 VIGYAYFLFTSRDPTYQDLLKRLFLSRQRKLFKKHSFDVCRFKELQKKCK------SPLD 362
           ++GYAYFL TSRDPTYQD +KRLFLSRQRKL K H FD  RFKEL+ K K      S   
Sbjct: 209 IVGYAYFLMTSRDPTYQDFMKRLFLSRQRKLLKSHKFDAERFKELENKWKITSCSSSSCH 268

Query: 363 ATANIKNRVGVDLDLEDCL 375
           A A+I+NRVGVDLDLED L
Sbjct: 269 ANASIRNRVGVDLDLEDSL 287

BLAST of Lsi10G014940 vs. TAIR 10
Match: AT1G57610.2 (Protein of unknown function (DUF607) )

HSP 1 Score: 308.9 bits (790), Expect = 5.5e-84
Identity = 160/259 (61.78%), Postives = 199/259 (76.83%), Query Frame = 0

Query: 123 GLGSVNEESGDGIGGETISFGEAKRLMRLVNVEALKMKL-GTDGKEAIGYSELLEACKSM 182
           G   +N ES +    + ++  EAK+LMRLVNVE +K KL G   KE + Y+ L+EA + +
Sbjct: 29  GFRCLNVESKEEDEKKDMTVLEAKKLMRLVNVEDMKKKLIGMGDKEMVTYTTLIEASQGL 88

Query: 183 GVARSQDEAAAFARVLDEAGVVLLFRDKVYLHPDKVVDLVRRAIPIALMPEDDPTRSELK 242
           G+ARS DEA AFARVLD+AGV+L+FRDKVYLHP KVVDL+R+A+P+ L P+D+  R E  
Sbjct: 89  GIARSLDEAHAFARVLDDAGVILIFRDKVYLHPHKVVDLIRKAVPLGLNPDDELIREEFD 148

Query: 243 QLQEKKEEIDVLAHKQVRRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTTGL 302
           +++  KEEIDVLAH+QVR+ILW GLG +++Q+G+F RLTFWEFSWDVMEPI FFTT TG+
Sbjct: 149 KMRSMKEEIDVLAHQQVRKILWGGLGYSVVQIGIFVRLTFWEFSWDVMEPITFFTTATGI 208

Query: 303 VIGYAYFLFTSRDPTYQDLLKRLFLSRQRKLFKKHSFDVCRFKELQKKCK------SPLD 362
           ++GYAYFL TSRDPTYQD +KRLFLSRQRKL K H FD  RFKEL+ K K      S   
Sbjct: 209 IVGYAYFLMTSRDPTYQDFMKRLFLSRQRKLLKSHKFDAERFKELENKWKITSCSSSSCH 268

Query: 363 ATANIKNRVGVDLDLEDCL 375
           A A+I+NRVGVDLDLED L
Sbjct: 269 ANASIRNRVGVDLDLEDSL 287

BLAST of Lsi10G014940 vs. TAIR 10
Match: AT1G57610.3 (Protein of unknown function (DUF607) )

HSP 1 Score: 213.0 bits (541), Expect = 4.1e-55
Identity = 107/181 (59.12%), Postives = 141/181 (77.90%), Query Frame = 0

Query: 123 GLGSVNEESGDGIGGETISFGEAKRLMRLVNVEALKMKL-GTDGKEAIGYSELLEACKSM 182
           G   +N ES +    + ++  EAK+LMRLVNVE +K KL G   KE + Y+ L+EA + +
Sbjct: 29  GFRCLNVESKEEDEKKDMTVLEAKKLMRLVNVEDMKKKLIGMGDKEMVTYTTLIEASQGL 88

Query: 183 GVARSQDEAAAFARVLDEAGVVLLFRDKVYLHPDKVVDLVRRAIPIALMPEDDPTRSELK 242
           G+ARS DEA AFARVLD+AGV+L+FRDKVYLHP KVVDL+R+A+P+ L P+D+  R E  
Sbjct: 89  GIARSLDEAHAFARVLDDAGVILIFRDKVYLHPHKVVDLIRKAVPLGLNPDDELIREEFD 148

Query: 243 QLQEKKEEIDVLAHKQVRRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTTGL 302
           +++  KEEIDVLAH+QVR+ILW GLG +++Q+G+F RLTFWEFSWDVMEPI FFTT TG+
Sbjct: 149 KMRSMKEEIDVLAHQQVRKILWGGLGYSVVQIGIFVRLTFWEFSWDVMEPITFFTTATGI 208

BLAST of Lsi10G014940 vs. TAIR 10
Match: AT2G23790.1 (Protein of unknown function (DUF607) )

HSP 1 Score: 157.9 bits (398), Expect = 1.6e-38
Identity = 87/225 (38.67%), Postives = 134/225 (59.56%), Query Frame = 0

Query: 139 TISFGEAKRLMRLVNVEALKMKLGTDGKEAIGYSELLEACKSMGVARSQ-DEAAAFARVL 198
           +++  + K+L+R   +E +K KL   G+  + Y E +  C   G A S  D  +  A++L
Sbjct: 109 SLTVADTKKLLRAAQIEIVKSKLRETGRSWMPYKEFVSVC---GEASSDPDLGSKIAKML 168

Query: 199 DEAGVVLLFRDKVYLHPDKVVDLVRRAIPI-ALMPEDDPTRSELKQLQEKKEEIDVLAHK 258
           D++  V++  D V + PD+V   +   +P+  +   +DP R E K+L+ +K  IDV AH 
Sbjct: 169 DDSANVIVLGDSVCIRPDQVTKSIEGLLPLPKIHNPNDPRRIEFKELEAEKAVIDVKAHT 228

Query: 259 QVRRILWTGLGLAILQVGLFFRLTFWEFSWDVMEPIAFFTTTTGLVIGYAYFLFTSRDPT 318
            VR+ LW GLG  ILQ   F RLTFWE SWDVMEPI F+ T+   + GYA+FL TS++P+
Sbjct: 229 LVRKELWAGLGYLILQTAGFMRLTFWELSWDVMEPICFYVTSVYFMAGYAFFLRTSKEPS 288

Query: 319 YQDLLKRLFLSRQRKLFKKHSFDVCRFKELQKK-CKSPLDATANI 361
           ++   +  F ++QRKL  ++ FD+ R+ EL+K  C  P D  + I
Sbjct: 289 FEGFYQSRFEAKQRKLMNEYEFDLERYNELKKLFCSKPSDHVSKI 330

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I1112.7e-8365.97Calcium uniporter protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q8VYR07.8e-8361.78Calcium uniporter protein 5, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
O648232.2e-3738.67Calcium uniporter protein 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q1PE153.6e-3539.81Calcium uniporter protein 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q9LVR51.0e-3440.19Calcium uniporter protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Match NameE-valueIdentityDescription
A0A6J1FV501.5e-16690.72Calcium uniporter protein OS=Cucurbita moschata OX=3662 GN=LOC111448718 PE=3 SV=... [more]
A0A6J1JHV81.7e-16590.14Calcium uniporter protein OS=Cucurbita maxima OX=3661 GN=LOC111484499 PE=3 SV=1[more]
A0A5A7SU812.5e-16189.60Calcium uniporter protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3C2X12.5e-16189.60Calcium uniporter protein OS=Cucumis melo OX=3656 GN=LOC103496260 PE=3 SV=1[more]
A0A0A0KD481.1e-15988.44Calcium uniporter protein OS=Cucumis sativus OX=3659 GN=Csa_6G133830 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038902671.11.7e-18091.27calcium uniporter protein 5, mitochondrial-like [Benincasa hispida][more]
KAG6570611.11.1e-16691.01Calcium uniporter protein 5, mitochondrial, partial [Cucurbita argyrosperma subs... [more]
XP_022944197.13.2e-16690.72calcium uniporter protein 5, mitochondrial-like [Cucurbita moschata][more]
XP_023512709.11.6e-16590.49calcium uniporter protein 5, mitochondrial-like [Cucurbita pepo subsp. pepo][more]
XP_022986898.13.5e-16590.14calcium uniporter protein 5, mitochondrial-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G09575.11.9e-8465.97Protein of unknown function (DUF607) [more]
AT1G57610.15.5e-8461.78Protein of unknown function (DUF607) [more]
AT1G57610.25.5e-8461.78Protein of unknown function (DUF607) [more]
AT1G57610.34.1e-5559.12Protein of unknown function (DUF607) [more]
AT2G23790.11.6e-3838.67Protein of unknown function (DUF607) [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006769Calcium uniporter protein, C-terminalPFAMPF04678MCUcoord: 187..346
e-value: 2.9E-50
score: 170.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 107..131
NoneNo IPR availablePANTHERPTHR13462:SF42SUBFAMILY NOT NAMEDcoord: 140..357
IPR039055MCU familyPANTHERPTHR13462CALCIUM UNIPORTER PROTEIN, MITOCHONDRIALcoord: 140..357

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G014940.1Lsi10G014940.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0036444 calcium import into the mitochondrion
biological_process GO:0051560 mitochondrial calcium ion homeostasis
cellular_component GO:0031305 integral component of mitochondrial inner membrane
cellular_component GO:1990246 uniplex complex
molecular_function GO:0005262 calcium channel activity
molecular_function GO:0015292 uniporter activity