Lsi10G014150 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G014150
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionUPF0503 protein At3g09070, chloroplastic
Locationchr10: 18249670 .. 18251394 (+)
RNA-Seq ExpressionLsi10G014150
SyntenyLsi10G014150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATCTGCAGCTCAAAACTGTATCCCATCGGCTTTCAACTTGTCACCGTCATCCTAGCAAGCCGGTGACTGGATTCTGCGCCTCTTGTCTCCGGGAACGCCTTGCTGGGATTGATCCCGACACGCAGCAGGAATCGCCTGTCCGGAACAACCATTTTTCATCGGAGCTTCGTCGGAGTAAATCCTTTTCCGCAGCGAAGTGTGAGGCTGGTATTGGACAATCGGAGGTTCAGCATCGGAAGTCTTGCGATGTTCGGTCTGGGAACTCCTTGTCGGACCTTTTCTGTCGCGAAGATAAACCGAGATGTACGAATCGGGAGGTGGAGATCGAATCCGAGAATTTAGGTTTTGAATTGCGTGAGGTTGTGGCTAATGAGAGACAATTTAGGGCTTCCGAGGGGATAATTGGACCGGCTTTGGGCACGATCGATGATTTTTCTGGAGATGAGGCTGAGTTCAAGACGGTGAAAGAGTTCATAGATATTGAATTTCGGAGGAAGAAGAATGCAGGTCGAGATTTACGAGAAATTGCAGGGAGTGTTTGGGAAGCGGCTTCAGTCTTCAGCAAGAAACTCGGCAAATGGAGGAAAAAGCAAAAAAGGAAGAATCTCAGTAACAATAGCAATGTAGGTGCGGTGAAAGCAGAGGATATCAAGCCTAGAGAGCTTGAAATCAGGGAAACTCGTTCGGAGGTTGGAGAATATGGATTGGGAAGAAGGTCTTGTGATACAGATCCAAGATTCTCTGTCGATGCAGGTAGAATGTCGTTGGATGATTCACGGTATTCATTCGATGAGCCAAGGGCTTCTTGGGATGGGTATCTGATTGGAAAAACTTATCCAAGGATTACGCCGATGGTATCAGTTTTGGAGGAGGCCAAATTTTCTGGTACTGGATTTGAGAAAGATGCTCCTTCCGATGAAGCAGAAGGGTCTCCGATGAATGTAGGAGATAAGATCCCTGGTGGATCGGCTCAGACTAAAGATTACTACATGGATTCATTGTCTTCTCTGAGGCGGAGGAAGAGTTTTGATCGTTCAAGCTCACACAGAAAAGGGGCATCAGGAGACTTTGATGACTTGAAATTAATATCAAACGCAAAGGTATCTCCTGCAACTACAGAGTTGTTCTATGGTGCAAAGGTTCTAATCACAGAGAAAGATTTGAACAACTCTCGCTCAAAAGCAACCAGAGATGGCGATTTGAGTGGCACTGATGTTACTTCCAAAGATTCTGTTCCTGATGCAGCTGTGGTTGATCGAAAGACATTCAAGAAGGTGCATAGATGGCGTAAAGTATTAAGTGTTCTGGGTATGATCCAAAAGCGAAGTGGTGAAAGCAAATCTGATGATGAAGAAAGTAGTGTTGGAGGTAATGTCGTTGATCGGCCTATAGCCGAGTCTTGGGAAAAGCTGAGGCGTGTTGCTAATGGAGAAGCAAACTCTTGTGTTAGCCAGAAGCTCATTCGCAGTTACAGTGTAAGCTGTCGAGATCCCAGCAAACTAGCTGGATTTAATGGCAGTAATGATTCGAAACTGAACGTTTCGAGATGGAGAGACGATTTTACATTGCAGAGGAATCGGAGTGTCAGGTATTCGCCAAATAACTTTGATAATGGCTTATTAAGGTTTTATTTGACACCATTGAGGAGCTACAGTAGCAGAGGCAAACCAGGAAAGAGCAGACCAAGAAGTTCTCCTTTCAATGTCAAGCATGTCATGTAA

mRNA sequence

ATGAATCTGCAGCTCAAAACTGTATCCCATCGGCTTTCAACTTGTCACCGTCATCCTAGCAAGCCGGTGACTGGATTCTGCGCCTCTTGTCTCCGGGAACGCCTTGCTGGGATTGATCCCGACACGCAGCAGGAATCGCCTGTCCGGAACAACCATTTTTCATCGGAGCTTCGTCGGAGTAAATCCTTTTCCGCAGCGAAGTGTGAGGCTGGTATTGGACAATCGGAGGTTCAGCATCGGAAGTCTTGCGATGTTCGGTCTGGGAACTCCTTGTCGGACCTTTTCTGTCGCGAAGATAAACCGAGATGTACGAATCGGGAGGTGGAGATCGAATCCGAGAATTTAGGTTTTGAATTGCGTGAGGTTGTGGCTAATGAGAGACAATTTAGGGCTTCCGAGGGGATAATTGGACCGGCTTTGGGCACGATCGATGATTTTTCTGGAGATGAGGCTGAGTTCAAGACGGTGAAAGAGTTCATAGATATTGAATTTCGGAGGAAGAAGAATGCAGGTCGAGATTTACGAGAAATTGCAGGGAGTGTTTGGGAAGCGGCTTCAGTCTTCAGCAAGAAACTCGGCAAATGGAGGAAAAAGCAAAAAAGGAAGAATCTCAGTAACAATAGCAATGTAGGTGCGGTGAAAGCAGAGGATATCAAGCCTAGAGAGCTTGAAATCAGGGAAACTCGTTCGGAGGTTGGAGAATATGGATTGGGAAGAAGGTCTTGTGATACAGATCCAAGATTCTCTGTCGATGCAGGTAGAATGTCGTTGGATGATTCACGGTATTCATTCGATGAGCCAAGGGCTTCTTGGGATGGGTATCTGATTGGAAAAACTTATCCAAGGATTACGCCGATGGTATCAGTTTTGGAGGAGGCCAAATTTTCTGGTACTGGATTTGAGAAAGATGCTCCTTCCGATGAAGCAGAAGGGTCTCCGATGAATGTAGGAGATAAGATCCCTGGTGGATCGGCTCAGACTAAAGATTACTACATGGATTCATTGTCTTCTCTGAGGCGGAGGAAGAGTTTTGATCGTTCAAGCTCACACAGAAAAGGGGCATCAGGAGACTTTGATGACTTGAAATTAATATCAAACGCAAAGGTATCTCCTGCAACTACAGAGTTGTTCTATGGTGCAAAGGTTCTAATCACAGAGAAAGATTTGAACAACTCTCGCTCAAAAGCAACCAGAGATGGCGATTTGAGTGGCACTGATGTTACTTCCAAAGATTCTGTTCCTGATGCAGCTGTGGTTGATCGAAAGACATTCAAGAAGGTGCATAGATGGCGTAAAGTATTAAGTGTTCTGGGTATGATCCAAAAGCGAAGTGGTGAAAGCAAATCTGATGATGAAGAAAGTAGTGTTGGAGGTAATGTCGTTGATCGGCCTATAGCCGAGTCTTGGGAAAAGCTGAGGCGTGTTGCTAATGGAGAAGCAAACTCTTGTGTTAGCCAGAAGCTCATTCGCAGTTACAGTGTAAGCTGTCGAGATCCCAGCAAACTAGCTGGATTTAATGGCAGTAATGATTCGAAACTGAACGTTTCGAGATGGAGAGACGATTTTACATTGCAGAGGAATCGGAGTGTCAGGTATTCGCCAAATAACTTTGATAATGGCTTATTAAGGTTTTATTTGACACCATTGAGGAGCTACAGTAGCAGAGGCAAACCAGGAAAGAGCAGACCAAGAAGTTCTCCTTTCAATGTCAAGCATGTCATGTAA

Coding sequence (CDS)

ATGAATCTGCAGCTCAAAACTGTATCCCATCGGCTTTCAACTTGTCACCGTCATCCTAGCAAGCCGGTGACTGGATTCTGCGCCTCTTGTCTCCGGGAACGCCTTGCTGGGATTGATCCCGACACGCAGCAGGAATCGCCTGTCCGGAACAACCATTTTTCATCGGAGCTTCGTCGGAGTAAATCCTTTTCCGCAGCGAAGTGTGAGGCTGGTATTGGACAATCGGAGGTTCAGCATCGGAAGTCTTGCGATGTTCGGTCTGGGAACTCCTTGTCGGACCTTTTCTGTCGCGAAGATAAACCGAGATGTACGAATCGGGAGGTGGAGATCGAATCCGAGAATTTAGGTTTTGAATTGCGTGAGGTTGTGGCTAATGAGAGACAATTTAGGGCTTCCGAGGGGATAATTGGACCGGCTTTGGGCACGATCGATGATTTTTCTGGAGATGAGGCTGAGTTCAAGACGGTGAAAGAGTTCATAGATATTGAATTTCGGAGGAAGAAGAATGCAGGTCGAGATTTACGAGAAATTGCAGGGAGTGTTTGGGAAGCGGCTTCAGTCTTCAGCAAGAAACTCGGCAAATGGAGGAAAAAGCAAAAAAGGAAGAATCTCAGTAACAATAGCAATGTAGGTGCGGTGAAAGCAGAGGATATCAAGCCTAGAGAGCTTGAAATCAGGGAAACTCGTTCGGAGGTTGGAGAATATGGATTGGGAAGAAGGTCTTGTGATACAGATCCAAGATTCTCTGTCGATGCAGGTAGAATGTCGTTGGATGATTCACGGTATTCATTCGATGAGCCAAGGGCTTCTTGGGATGGGTATCTGATTGGAAAAACTTATCCAAGGATTACGCCGATGGTATCAGTTTTGGAGGAGGCCAAATTTTCTGGTACTGGATTTGAGAAAGATGCTCCTTCCGATGAAGCAGAAGGGTCTCCGATGAATGTAGGAGATAAGATCCCTGGTGGATCGGCTCAGACTAAAGATTACTACATGGATTCATTGTCTTCTCTGAGGCGGAGGAAGAGTTTTGATCGTTCAAGCTCACACAGAAAAGGGGCATCAGGAGACTTTGATGACTTGAAATTAATATCAAACGCAAAGGTATCTCCTGCAACTACAGAGTTGTTCTATGGTGCAAAGGTTCTAATCACAGAGAAAGATTTGAACAACTCTCGCTCAAAAGCAACCAGAGATGGCGATTTGAGTGGCACTGATGTTACTTCCAAAGATTCTGTTCCTGATGCAGCTGTGGTTGATCGAAAGACATTCAAGAAGGTGCATAGATGGCGTAAAGTATTAAGTGTTCTGGGTATGATCCAAAAGCGAAGTGGTGAAAGCAAATCTGATGATGAAGAAAGTAGTGTTGGAGGTAATGTCGTTGATCGGCCTATAGCCGAGTCTTGGGAAAAGCTGAGGCGTGTTGCTAATGGAGAAGCAAACTCTTGTGTTAGCCAGAAGCTCATTCGCAGTTACAGTGTAAGCTGTCGAGATCCCAGCAAACTAGCTGGATTTAATGGCAGTAATGATTCGAAACTGAACGTTTCGAGATGGAGAGACGATTTTACATTGCAGAGGAATCGGAGTGTCAGGTATTCGCCAAATAACTTTGATAATGGCTTATTAAGGTTTTATTTGACACCATTGAGGAGCTACAGTAGCAGAGGCAAACCAGGAAAGAGCAGACCAAGAAGTTCTCCTTTCAATGTCAAGCATGTCATGTAA

Protein sequence

MNLQLKTVSHRLSTCHRHPSKPVTGFCASCLRERLAGIDPDTQQESPVRNNHFSSELRRSKSFSAAKCEAGIGQSEVQHRKSCDVRSGNSLSDLFCREDKPRCTNREVEIESENLGFELREVVANERQFRASEGIIGPALGTIDDFSGDEAEFKTVKEFIDIEFRRKKNAGRDLREIAGSVWEAASVFSKKLGKWRKKQKRKNLSNNSNVGAVKAEDIKPRELEIRETRSEVGEYGLGRRSCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGTGFEKDAPSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRSSSHRKGASGDFDDLKLISNAKVSPATTELFYGAKVLITEKDLNNSRSKATRDGDLSGTDVTSKDSVPDAAVVDRKTFKKVHRWRKVLSVLGMIQKRSGESKSDDEESSVGGNVVDRPIAESWEKLRRVANGEANSCVSQKLIRSYSVSCRDPSKLAGFNGSNDSKLNVSRWRDDFTLQRNRSVRYSPNNFDNGLLRFYLTPLRSYSSRGKPGKSRPRSSPFNVKHVM
Homology
BLAST of Lsi10G014150 vs. ExPASy Swiss-Prot
Match: Q9SS80 (Protein OCTOPUS OS=Arabidopsis thaliana OX=3702 GN=OPS PE=1 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 1.2e-61
Identity = 227/671 (33.83%), Postives = 306/671 (45.60%), Query Frame = 0

Query: 10  HRLST-CHRHPSKPVTGFCASCLRERLAGIDPDTQQES--------------------PV 69
           HRLST C+RHP +  TGFC SCL ERL+ +D      S                    P 
Sbjct: 25  HRLSTSCNRHPEERFTGFCPSCLCERLSVLDQTNNGGSSSSSKKPPTISAAALKALFKPS 84

Query: 70  RNN---------------HFSSELRRSKSFSAAKCEAGIGQSEVQHRKSCDVRSGNSLSD 129
            NN                F  ELRR+KSFSA+K   G        R+SCDVR  +SL +
Sbjct: 85  GNNGVGGVNTNGNGRVKPGFFPELRRTKSFSASKNNEGFSGVFEPQRRSCDVRLRSSLWN 144

Query: 130 LFCRED---------------KPRCT---------NREVEIESENLGFELRE----VVAN 189
           LF +++               +PR +         N E E ES++   E  E    V A 
Sbjct: 145 LFSQDEQRNLPSNVTGGEIDVEPRKSSVAEPVLEVNDEGEAESDDEELEEEEEEDYVEAG 204

Query: 190 ERQFRASEG-------------------IIGPALGTIDDFSGDEAEFKTVKEFIDIEFRR 249
           + +     G                    + P  G        E E K +K++ID++ + 
Sbjct: 205 DFEILNDSGELMREKSDEIVEVREEIEEAVKPTKGL------SEEELKPIKDYIDLDSQT 264

Query: 250 KKNAGRDLREIAGSVWEAASVFSKKLGKWRKKQKRKNLSNNSN--VGAVKAEDIKPRELE 309
           KK +      +  S W AASVFSKKL KWR+ QK K   N  +   G+ +    KP   +
Sbjct: 265 KKPS------VRRSFWSAASVFSKKLQKWRQNQKMKKRRNGGDHRPGSARLPVEKPIGRQ 324

Query: 310 IRETRSEVGEYGLGRRSCDTDPRFSVDA--------------GRMSLDDSRYSFDEPRAS 369
           +R+T+SE+ +YG GRRSCDTDPRFS+DA              GR+SLDD RYSFDEPRAS
Sbjct: 325 LRDTQSEIADYGYGRRSCDTDPRFSLDAGRFSLDAGRFSVDIGRISLDDPRYSFDEPRAS 384

Query: 370 WDGYLIGKTY-------PRITPMVSVLEEA----------KFSGTGFEKDAPSDEAEGSP 429
           WDG LIG+T        P    M+SV+E+A                 E+ AP        
Sbjct: 385 WDGSLIGRTMFPPAARAPPPPSMLSVVEDAPPPVHRHVTRADMQFPVEEPAPPPPVVNQT 444

Query: 430 MNVGDK--IPGGSAQTKDYYMDSLSSLRRRKSFDRSSSH-RKGAS---GDFDDLKLISNA 489
             V D   IPGGS QT+DYY D  SS RRRKS DRSSS  RK A+    D D+ KL    
Sbjct: 445 NGVSDPVIIPGGSIQTRDYYTD--SSSRRRKSLDRSSSSMRKTAAAVVADMDEPKL---- 504

Query: 490 KVSPATTELFYGAKVLITEKDLNNSRSKATRDGDLSGTDVTSKDSVPDAAVVDRKTFKKV 549
            VS A +   Y   +    +D NN   +   +G      +     + D  V      KK 
Sbjct: 505 SVSSAISIDAYSGSL----RDNNNYAVETADNGSFREPAM----MIGDRKVNSNDNNKKS 564

Query: 550 HRWRKVLSVLGMIQKRSGESKSDDEESS------VGGNVVDRPIAESWEKLRRVANGEAN 552
            RW K  S+LG+I ++S     ++EE        + G +V+R ++ESW +LR    G   
Sbjct: 565 RRWGK-WSILGLIYRKSVNKYEEEEEEEEDRYRRLNGGMVERSLSESWPELRNGGGGGG- 624

BLAST of Lsi10G014150 vs. ExPASy Swiss-Prot
Match: Q9LFB9 (Protein OCTOPUS-like OS=Arabidopsis thaliana OX=3702 GN=OPSL1 PE=2 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 1.8e-54
Identity = 203/602 (33.72%), Postives = 284/602 (47.18%), Query Frame = 0

Query: 10  HRLST-CHRHPSKPVTGFCASCLRERLAGID------PDTQQESP--------------- 69
           HRLST C  HP +  +GFC SCL +RL+ +D      P +    P               
Sbjct: 23  HRLSTSCDLHPEERFSGFCPSCLCDRLSVLDHNAAPPPSSSSRKPPSISAVSLKALFKPS 82

Query: 70  ---VRNNH--------FSSELRRSKSFSAAKCEAGIGQSEVQHRKSCDVRSGNSLSDLFC 129
                N++        F  ELRR+KSFSA   E   G  E Q R+SCDVR  +   +L  
Sbjct: 83  SSGTNNSNGNGRVRPGFFPELRRTKSFSAKNNEGFSGGFEPQ-RRSCDVRLRDDERNLPI 142

Query: 130 RE----DKPRCTNREVEIESENLGF-ELREVVANERQFRASEGIIGPALGTIDDFSGDEA 189
            E    DK     RE  +    L   E  E+  +E       G I     +  +   +E 
Sbjct: 143 NEAASVDKIEEEARESSVSEIVLEVTEEAEIEEDEENGEKDPGEIVEEKSS--EIGEEEE 202

Query: 190 EFKTVKEFIDIEFRRKKNAGRDLREIAGSVWEAASVFSKKLGKWRKKQKRKNLSNNSNVG 249
           E K +K+++D+  + KK +   +++ AGS + AASVFSKKL KW++KQK K   N    G
Sbjct: 203 ELKPMKDYMDLYSQTKKPS---VKDFAGSFFSAASVFSKKLQKWKQKQKVKKPRNGVGGG 262

Query: 250 AVKAEDIKPRELEIRETRSEVGEYGLGRRSCDTDPRFSVDA-------GRMSLDDSRYSF 309
                  +P+            E G+GRRS DTDPRFS+DA       GR+S+DDSRYS 
Sbjct: 263 -------RPQ-----------SEIGVGRRSSDTDPRFSLDAGRFSVDIGRISMDDSRYSL 322

Query: 310 DEPRASWDGYLIGKTYPRITP----MVSVLEEAKFSGTGFE-KDAPSDEAEGSPMNVGDK 369
           DEPRASWDG+LIG+T     P    M+SV+E A  + +  +   +PS +      +    
Sbjct: 323 DEPRASWDGHLIGRTTAARVPLPPSMLSVVENAPLNRSDMQIPSSPSIKPISGDSDPIII 382

Query: 370 IPGGSAQTKDYYMDSLSSLRRRKSFDRSSSHRKGASGDFDDLKLISNAKVSPATTELFYG 429
           IPGGS QT+DYY    SS RRRKS DRS+S RK  + + +D+K +SN+  +         
Sbjct: 383 IPGGSNQTRDYYTGPPSS-RRRKSLDRSNSIRKIVT-ELEDVKSVSNSTTT--------- 442

Query: 430 AKVLITEKDLNNSRSKATRDGDLSGTDVTSKDSVPDAAVVDRKTFKKVHRWRKVLSVLGM 489
               I    +  + +K  ++GD                       KK  RW K  S+LG 
Sbjct: 443 ----IDSNSMETAENKGNQNGD-----------------------KKSRRWGK-WSILGF 502

Query: 490 IQKRSGESKSDDEES-SVGGNVVDRPIAESWEKLRRVANGEANSCVSQKLIRSYSVSCRD 549
           I ++  + + +D  S S    +V+R ++ESW ++R   NGE       K+ RS S     
Sbjct: 503 IYRKGKDDEEEDRYSRSNSAGMVERSLSESWPEMR---NGEGG---GPKMRRSNS----- 535

Query: 550 PSKLAGFNGSNDSKLNVSRWRDDFTLQRNRSVRYSPNNFDNGLLRFYLTPL-RSYSSRGK 560
                          NVS         RN+S RYS  + +NG+LRFYLTP+ RS+ + G 
Sbjct: 563 ---------------NVSWRSSGGGSARNKSSRYSSKDGENGMLRFYLTPMRRSWKTSGG 535

BLAST of Lsi10G014150 vs. ExPASy TrEMBL
Match: A0A5A7V3J1 (UPF0503 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold571G00450 PE=4 SV=1)

HSP 1 Score: 1056.2 bits (2730), Expect = 4.9e-305
Identity = 534/575 (92.87%), Postives = 551/575 (95.83%), Query Frame = 0

Query: 1   MNLQLKTVSHRLSTCHRHPSKPVTGFCASCLRERLAGIDPDTQQESPVRNNHFSS-ELRR 60
           MNLQLK+VSHRLSTCHRHPSKPVTGFCASCLRERLAGIDPD Q ESP+ NNH SS ELRR
Sbjct: 1   MNLQLKSVSHRLSTCHRHPSKPVTGFCASCLRERLAGIDPDLQHESPLPNNHSSSAELRR 60

Query: 61  SKSFSAAKCEAGIGQSEVQHRKSCDVRSGNSLSDLFCREDKPRCTNREVEIESENLGFEL 120
           SKS+SAAKCEAGIGQSE+QHRKSCDVRSGNSLSDLFCREDKPRCTN EVEIESENLGFEL
Sbjct: 61  SKSYSAAKCEAGIGQSELQHRKSCDVRSGNSLSDLFCREDKPRCTNPEVEIESENLGFEL 120

Query: 121 REVVANERQFRASEGIIGPALGTIDDFSGDEAEFKTVKEFIDIEFRRKKNAGRDLREIAG 180
           REVV N RQFRASEGIIGP LGTIDDF+G++AEFKTVKEFID+EFRRKKNAGRDLREIAG
Sbjct: 121 REVVGNGRQFRASEGIIGPGLGTIDDFAGEDAEFKTVKEFIDLEFRRKKNAGRDLREIAG 180

Query: 181 SVWEAASVFSKKLGKWRKKQKRKNLSNNSNVGAVKAEDIKPRELEIRETRSEVGEYGLGR 240
           SVWEAASVFSKKL KWRKKQKRKNL NNSNVGAVK EDIKPR LEIRETRSEVGEYGLGR
Sbjct: 181 SVWEAASVFSKKLSKWRKKQKRKNLGNNSNVGAVKVEDIKPRALEIRETRSEVGEYGLGR 240

Query: 241 RSCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGTG 300
           RSCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSG G
Sbjct: 241 RSCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGAG 300

Query: 301 FEKDAPSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRSSSHRKGASGDFD 360
           FEKD PSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRS SHRKGASGDFD
Sbjct: 301 FEKDDPSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRSCSHRKGASGDFD 360

Query: 361 DLKLISNAKVSPATTELFYGAKVLITEKDLNNSRSKATRDGDLSGTDVTSKDSVPDAAVV 420
           +LKLISNAKVSPATTELFYGAKVLITEKDLN+SR KAT DGDLSGTDVTSKDSVPDA V+
Sbjct: 361 ELKLISNAKVSPATTELFYGAKVLITEKDLNSSRPKATGDGDLSGTDVTSKDSVPDAPVI 420

Query: 421 DRKTFKKVHRWRKVLSVLGMIQKRSGESKSDDEESSVGGNVVDRPIAESWEKLRRVANGE 480
           DRK+FKKVHRWRKVLSVLGMIQKR+GESKSDDEESSV GNVVDRP+ ESWEKLRRVANGE
Sbjct: 421 DRKSFKKVHRWRKVLSVLGMIQKRNGESKSDDEESSVAGNVVDRPVVESWEKLRRVANGE 480

Query: 481 ANSCVSQKLIRSYSVSCRDPSKLAGFNGSNDSKLNVSRWRDDFTLQRNRSVRYSPNNFDN 540
           ANSCVSQKLIRSYSVSCRDPSKLAGFNG NDSKLNV+RWRDDFTLQRNRSVRYSPNNFDN
Sbjct: 481 ANSCVSQKLIRSYSVSCRDPSKLAGFNGGNDSKLNVTRWRDDFTLQRNRSVRYSPNNFDN 540

Query: 541 GLLRFYLTPLRSYSSRGKPGKSRPRSSPFNVKHVM 575
           GLLRFYLTPLRSY SRGK GKSRPR+SPFNVKHV+
Sbjct: 541 GLLRFYLTPLRSY-SRGKLGKSRPRNSPFNVKHVI 574

BLAST of Lsi10G014150 vs. ExPASy TrEMBL
Match: A0A1S3CKL0 (UPF0503 protein At3g09070, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103502039 PE=4 SV=1)

HSP 1 Score: 1056.2 bits (2730), Expect = 4.9e-305
Identity = 534/575 (92.87%), Postives = 551/575 (95.83%), Query Frame = 0

Query: 1   MNLQLKTVSHRLSTCHRHPSKPVTGFCASCLRERLAGIDPDTQQESPVRNNHFSS-ELRR 60
           MNLQLK+VSHRLSTCHRHPSKPVTGFCASCLRERLAGIDPD Q ESP+ NNH SS ELRR
Sbjct: 1   MNLQLKSVSHRLSTCHRHPSKPVTGFCASCLRERLAGIDPDLQHESPLPNNHSSSAELRR 60

Query: 61  SKSFSAAKCEAGIGQSEVQHRKSCDVRSGNSLSDLFCREDKPRCTNREVEIESENLGFEL 120
           SKS+SAAKCEAGIGQSE+QHRKSCDVRSGNSLSDLFCREDKPRCTN EVEIESENLGFEL
Sbjct: 61  SKSYSAAKCEAGIGQSELQHRKSCDVRSGNSLSDLFCREDKPRCTNPEVEIESENLGFEL 120

Query: 121 REVVANERQFRASEGIIGPALGTIDDFSGDEAEFKTVKEFIDIEFRRKKNAGRDLREIAG 180
           REVV N RQFRASEGIIGP LGTIDDF+G++AEFKTVKEFID+EFRRKKNAGRDLREIAG
Sbjct: 121 REVVGNGRQFRASEGIIGPGLGTIDDFAGEDAEFKTVKEFIDLEFRRKKNAGRDLREIAG 180

Query: 181 SVWEAASVFSKKLGKWRKKQKRKNLSNNSNVGAVKAEDIKPRELEIRETRSEVGEYGLGR 240
           SVWEAASVFSKKL KWRKKQKRKNL NNSNVGAVK EDIKPR LEIRETRSEVGEYGLGR
Sbjct: 181 SVWEAASVFSKKLSKWRKKQKRKNLGNNSNVGAVKVEDIKPRALEIRETRSEVGEYGLGR 240

Query: 241 RSCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGTG 300
           RSCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSG G
Sbjct: 241 RSCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGAG 300

Query: 301 FEKDAPSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRSSSHRKGASGDFD 360
           FEKD PSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRS SHRKGASGDFD
Sbjct: 301 FEKDDPSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRSCSHRKGASGDFD 360

Query: 361 DLKLISNAKVSPATTELFYGAKVLITEKDLNNSRSKATRDGDLSGTDVTSKDSVPDAAVV 420
           +LKLISNAKVSPATTELFYGAKVLITEKDLN+SR KAT DGDLSGTDVTSKDSVPDA V+
Sbjct: 361 ELKLISNAKVSPATTELFYGAKVLITEKDLNSSRPKATGDGDLSGTDVTSKDSVPDAPVI 420

Query: 421 DRKTFKKVHRWRKVLSVLGMIQKRSGESKSDDEESSVGGNVVDRPIAESWEKLRRVANGE 480
           DRK+FKKVHRWRKVLSVLGMIQKR+GESKSDDEESSV GNVVDRP+ ESWEKLRRVANGE
Sbjct: 421 DRKSFKKVHRWRKVLSVLGMIQKRNGESKSDDEESSVAGNVVDRPVVESWEKLRRVANGE 480

Query: 481 ANSCVSQKLIRSYSVSCRDPSKLAGFNGSNDSKLNVSRWRDDFTLQRNRSVRYSPNNFDN 540
           ANSCVSQKLIRSYSVSCRDPSKLAGFNG NDSKLNV+RWRDDFTLQRNRSVRYSPNNFDN
Sbjct: 481 ANSCVSQKLIRSYSVSCRDPSKLAGFNGGNDSKLNVTRWRDDFTLQRNRSVRYSPNNFDN 540

Query: 541 GLLRFYLTPLRSYSSRGKPGKSRPRSSPFNVKHVM 575
           GLLRFYLTPLRSY SRGK GKSRPR+SPFNVKHV+
Sbjct: 541 GLLRFYLTPLRSY-SRGKLGKSRPRNSPFNVKHVI 574

BLAST of Lsi10G014150 vs. ExPASy TrEMBL
Match: A0A0A0KBP2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G187980 PE=4 SV=1)

HSP 1 Score: 1054.7 bits (2726), Expect = 1.4e-304
Identity = 536/576 (93.06%), Postives = 553/576 (96.01%), Query Frame = 0

Query: 1   MNLQLKTVSHRLSTCHRHPSKPVTGFCASCLRERLAGIDPDTQQESPVRNNHFSSELRRS 60
           MNLQLK+VSHRLSTCHRHPSKPVTGFCASCLRERLAGIDPDTQ ESPV+NNH S+ELRRS
Sbjct: 1   MNLQLKSVSHRLSTCHRHPSKPVTGFCASCLRERLAGIDPDTQHESPVQNNHSSAELRRS 60

Query: 61  KSFSAAKCEA-GIGQSEVQHRKSCDVRSGNSLSDLFCREDKPRCTNREVEIESENLGFEL 120
           KS SAAKCEA GIGQSEVQHRKSCDVRSGNSLSDLFCREDKPRCTNREVEIESENLGFEL
Sbjct: 61  KSHSAAKCEAGGIGQSEVQHRKSCDVRSGNSLSDLFCREDKPRCTNREVEIESENLGFEL 120

Query: 121 REVVANERQFRASEGIIGPALGTIDDFSGDEAEFKTVKEFIDIEFRRKKNAGRDLREIAG 180
           REVV N RQFRASEGIIGP LGTID FSG+EAEFKTVKEFID+EFRRKKNAGRDLREIAG
Sbjct: 121 REVVGNGRQFRASEGIIGPGLGTIDGFSGEEAEFKTVKEFIDLEFRRKKNAGRDLREIAG 180

Query: 181 SVWEAASVFSKKLGKWRKKQKRKNLSNNSNVGAVKAEDIKPRELEIRETRSEVGEYGLGR 240
           SVWEAAS FSKKLGKWRKKQKRKNLSNNS VGAVKAEDIKPR  EIRETRSEVGEYGLGR
Sbjct: 181 SVWEAASGFSKKLGKWRKKQKRKNLSNNSYVGAVKAEDIKPRAHEIRETRSEVGEYGLGR 240

Query: 241 RSCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGTG 300
           RSCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGTG
Sbjct: 241 RSCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGTG 300

Query: 301 FEKDAPSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRSSSHRKGASGDFD 360
           FEKD PSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSS+RRRKSFDRS SHRKGASGDFD
Sbjct: 301 FEKDDPSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSSMRRRKSFDRSCSHRKGASGDFD 360

Query: 361 DLKLISNAKVSPATTELFYGAKVLITEKDLNNSRSKATRDGDLSGTDVTSKDSVPDAAVV 420
           +LKLISNAKVSPATTELFYGAKVLITEKDLN+SR K T DGDLSGTDVTSKDSVPDA V+
Sbjct: 361 ELKLISNAKVSPATTELFYGAKVLITEKDLNSSRPKTTGDGDLSGTDVTSKDSVPDAPVI 420

Query: 421 DRKTFKKVHRWRKVLSVLGMIQKRSGESKSDDEESSVGGNVVDRPIAESWEKLRRVANGE 480
           DRKTFKKVHRWRKVLSVLGM+QKR+GESKSDDEESSVGGNVVDRP+ ESWEKLRRVANGE
Sbjct: 421 DRKTFKKVHRWRKVLSVLGMVQKRNGESKSDDEESSVGGNVVDRPVVESWEKLRRVANGE 480

Query: 481 ANSCVSQKLIRSYSVSCRDPSKLAGFNGSNDSKLNVSRWRDDFTLQRNRSVRYSPNNFDN 540
           ANSCVSQKLIRSYSVSCRDPSKLAGFNG NDSKLNV+RWRDDFTLQRNRSVRYSPNNFDN
Sbjct: 481 ANSCVSQKLIRSYSVSCRDPSKLAGFNGGNDSKLNVTRWRDDFTLQRNRSVRYSPNNFDN 540

Query: 541 -GLLRFYLTPLRSYSSRGKPGKSRPRSSPFNVKHVM 575
            GLLRFYLTPLRSY +RGK GK+RPR+SPFNVKHV+
Sbjct: 541 GGLLRFYLTPLRSY-NRGKLGKNRPRNSPFNVKHVI 575

BLAST of Lsi10G014150 vs. ExPASy TrEMBL
Match: A0A6J1G914 (UPF0503 protein At3g09070, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111451814 PE=4 SV=1)

HSP 1 Score: 984.2 bits (2543), Expect = 2.3e-283
Identity = 506/575 (88.00%), Postives = 532/575 (92.52%), Query Frame = 0

Query: 1   MNLQLKTVSHRLSTCHRHPSKPVTGFCASCLRERLAGIDPDTQQESPVRNNHFSSELRRS 60
           MNLQ K VSHRLS+C RHPS+PVTGFCASCLRERLAGID DT+QESPV N H +SELRRS
Sbjct: 1   MNLQNKAVSHRLSSCRRHPSEPVTGFCASCLRERLAGIDSDTRQESPVLNQHSTSELRRS 60

Query: 61  KSFSAAKCEAGIGQSEVQHRKSCDVRSGNSLSDLFCREDKPRCTNREVEIESENLGFELR 120
           KSFSAAK EAGIG+ EVQHRKSCD RSGNSLSDLFCREDKPRCTNREVEIESENLGFELR
Sbjct: 61  KSFSAAKREAGIGRPEVQHRKSCDARSGNSLSDLFCREDKPRCTNREVEIESENLGFELR 120

Query: 121 EVVANERQFRASEGIIGPALGTIDDFSGDEAEFKTVKEFIDIEFRRKKNAGRDLREIAGS 180
           EVVA ERQFRASEG+IGPAL  IDDFSG++AEFKT+KEFID+EFRRKKNAGRDLREIAGS
Sbjct: 121 EVVAKERQFRASEGVIGPALDIIDDFSGEDAEFKTMKEFIDLEFRRKKNAGRDLREIAGS 180

Query: 181 VWEAASVFSKKLGKWRKKQKRKNLSNNSNVGAVKAEDIKPRELEIRETRSEVGEYGLGRR 240
           VWEAASVFSKKLGKWRKKQK KNL N+++VGA K E IKPR LE+RETRSEVGEYGLGRR
Sbjct: 181 VWEAASVFSKKLGKWRKKQKMKNLGNDTDVGAAKTEAIKPRVLEVRETRSEVGEYGLGRR 240

Query: 241 SCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGTGF 300
           SCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIG+T+PR TPMVSVLEE K  G GF
Sbjct: 241 SCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGRTHPRPTPMVSVLEETKLPGIGF 300

Query: 301 EKDAPSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRSSSHRKGASGDFDD 360
           EKD PSDEAEGSPMNVG+KIPGGSAQTKDYYMDSLS++RRRKSFDRSSSH+KGAS DFDD
Sbjct: 301 EKDDPSDEAEGSPMNVGEKIPGGSAQTKDYYMDSLSTVRRRKSFDRSSSHKKGASADFDD 360

Query: 361 LKLISNAKVSPATTELFYGAKVLITEKDLNNSRSKATRDGDLSGTDVTSKDSVPDAAVVD 420
           LKLISNAKVSPATTELFYGAKVLITEKDL +S SKATRDGDLSGT++TSKDSVPDAA +D
Sbjct: 361 LKLISNAKVSPATTELFYGAKVLITEKDLKDSHSKATRDGDLSGTNITSKDSVPDAAGID 420

Query: 421 RKTFKKVHRWRKVLSVLGMIQKRSGE-SKSDDEESSVGGNVVDRPI-AESWEKLRRVANG 480
           RKTFKKVHRWRKVLSVLGM+QKRSGE SKSDDEES VGGNVVDRP  AESWEKLRRVANG
Sbjct: 421 RKTFKKVHRWRKVLSVLGMLQKRSGESSKSDDEESCVGGNVVDRPFAAESWEKLRRVANG 480

Query: 481 EANSCVSQKLIRSYSVSCRDPSKLAGFN-GSNDSKLNVSRWRDDFTLQRNRSVRYSPNNF 540
           EAN  VSQKLIRSYSVSCRDPSKLAGFN G NDSKL   R RDDFTLQRNRS RYSPNNF
Sbjct: 481 EANGSVSQKLIRSYSVSCRDPSKLAGFNGGGNDSKLYGLRRRDDFTLQRNRSARYSPNNF 540

Query: 541 DNGLLRFYLTPLRSYSSRGKPGKSRPRSSPFNVKH 573
           DNGLLRFYLTPLRSY +RGKPGKSRPRSSPFNVKH
Sbjct: 541 DNGLLRFYLTPLRSY-NRGKPGKSRPRSSPFNVKH 574

BLAST of Lsi10G014150 vs. ExPASy TrEMBL
Match: A0A6J1FY88 (UPF0503 protein At3g09070, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111448423 PE=4 SV=1)

HSP 1 Score: 979.9 bits (2532), Expect = 4.4e-282
Identity = 503/574 (87.63%), Postives = 527/574 (91.81%), Query Frame = 0

Query: 1   MNLQLKTVSHRLSTCHRHPSKPVTGFCASCLRERLAGIDPDTQQESPVRNNHFSSELRRS 60
           MNLQ KTVSHRLS+CHRHP+KPVTGFCASCLRERLAGID DTQQESP++ +  S ELRRS
Sbjct: 1   MNLQAKTVSHRLSSCHRHPTKPVTGFCASCLRERLAGIDLDTQQESPLQKHLSSLELRRS 60

Query: 61  KSFSAAKCEAGIGQSEVQHRKSCDVRSGNSLSDLFCREDKPRCTNREVEIESENLGFELR 120
           KSFSAAK +A IG+ +VQHR+SCDVRSGNSLSDLFCREDKPRC N++VEIESENLGFEL 
Sbjct: 61  KSFSAAKRDASIGRMQVQHRRSCDVRSGNSLSDLFCREDKPRCRNQQVEIESENLGFELH 120

Query: 121 EVVANERQFRASEGIIGPALGTIDDFSGDEAEFKTVKEFIDIEFRRKKNAGRDLREIAGS 180
           EVVANERQFRAS G IGPALGTIDDF+G+EAEFKTVKEFID+EFRRKKNAGRDLREIAGS
Sbjct: 121 EVVANERQFRASGGTIGPALGTIDDFAGEEAEFKTVKEFIDLEFRRKKNAGRDLREIAGS 180

Query: 181 VWEAASVFSKKLGKWRKKQKRKNLSNNSNVGAVKAEDIKPRELEIRETRSEVGEYGLGRR 240
           VWEAASVFSKKLGKWRKKQKRKNL NN NV AV  E IK R LEI ETRSEVG+YGLGRR
Sbjct: 181 VWEAASVFSKKLGKWRKKQKRKNLGNNCNVDAVTTEAIKSRVLEIGETRSEVGDYGLGRR 240

Query: 241 SCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGTGF 300
           SCDTDPRFS D GRMSLDDSRYSFDE RASWDGYLIGKTYP+ITPMVSVLEEAKF GT F
Sbjct: 241 SCDTDPRFSADTGRMSLDDSRYSFDEARASWDGYLIGKTYPKITPMVSVLEEAKFPGTEF 300

Query: 301 EKDAPSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRSSSHRKGASGDFDD 360
           EKD PSDEAEGSP NVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRSSSHRKGAS DFDD
Sbjct: 301 EKDNPSDEAEGSPKNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRSSSHRKGASADFDD 360

Query: 361 LKLISNAKVSPATTELFYGAKVLITEKDLNNSRSKATRDGDLSGTDVTSKDSVPDAAVVD 420
           LKLISNAKVSPATTELFYGAKVL+TEKDLN SRSKATRDG+LSGTDVTSKDSV DAA +D
Sbjct: 361 LKLISNAKVSPATTELFYGAKVLLTEKDLNKSRSKATRDGELSGTDVTSKDSVLDAAGID 420

Query: 421 RKTFKKVHRWRKVLSVLGMIQKRSGESKSDDEESSVGGNVVDRPIAESWEKLRRVANGEA 480
           RKTFKKVHRWRKVLSVLGM+ KRSGESKSDDEES       DRPIAESWEKLRRVANGEA
Sbjct: 421 RKTFKKVHRWRKVLSVLGMMHKRSGESKSDDEES------CDRPIAESWEKLRRVANGEA 480

Query: 481 NSCVSQKLIRSYSVSCRDPSKLAGFNGSNDSKLNVSRWRDDFTLQRNRSVRYSPNNFDNG 540
           NSCVSQKLIRSYSVSCRD SK+AGFNG ND KLN SRWRDDFTL+RNRSVRYSPNNFDNG
Sbjct: 481 NSCVSQKLIRSYSVSCRDRSKVAGFNGGNDLKLNGSRWRDDFTLRRNRSVRYSPNNFDNG 540

Query: 541 LLRFYLTPLRSYSSRGKPGKSRPRSSPFNVKHVM 575
           LLRFYLTPLRS+ SRGKPGKSRPRSS FNVKHV+
Sbjct: 541 LLRFYLTPLRSH-SRGKPGKSRPRSSSFNVKHVV 567

BLAST of Lsi10G014150 vs. NCBI nr
Match: XP_038901013.1 (protein OCTOPUS [Benincasa hispida])

HSP 1 Score: 1087.8 bits (2812), Expect = 0.0e+00
Identity = 551/574 (95.99%), Postives = 560/574 (97.56%), Query Frame = 0

Query: 1   MNLQLKTVSHRLSTCHRHPSKPVTGFCASCLRERLAGIDPDTQQESPVRNNHFSSELRRS 60
           MNLQLKT+SHRLSTCHRHPSKPVTGFCASCLRERLAGID DTQQESPV NNH SSELRRS
Sbjct: 1   MNLQLKTLSHRLSTCHRHPSKPVTGFCASCLRERLAGIDTDTQQESPVPNNHSSSELRRS 60

Query: 61  KSFSAAKCEAGIGQSEVQHRKSCDVRSGNSLSDLFCREDKPRCTNREVEIESENLGFELR 120
           KS+SAAK EAGI QSEVQHRKSCDVRSGNSLSDLFCREDKPRCT REVEIESENLG ELR
Sbjct: 61  KSYSAAKREAGIEQSEVQHRKSCDVRSGNSLSDLFCREDKPRCTIREVEIESENLGSELR 120

Query: 121 EVVANERQFRASEGIIGPALGTIDDFSGDEAEFKTVKEFIDIEFRRKKNAGRDLREIAGS 180
           EVVANER FRASEGIIGPALGTIDDF+G+EAEFKTVKEFID+EFRRKKNAGRDLREIAGS
Sbjct: 121 EVVANERLFRASEGIIGPALGTIDDFAGEEAEFKTVKEFIDLEFRRKKNAGRDLREIAGS 180

Query: 181 VWEAASVFSKKLGKWRKKQKRKNLSNNSNVGAVKAEDIKPRELEIRETRSEVGEYGLGRR 240
           VWEAASVFSKKLGKWRKKQKRKNLSNN NVG VKAEDIKPR LEIRETRSEVG+YGLGRR
Sbjct: 181 VWEAASVFSKKLGKWRKKQKRKNLSNNGNVGTVKAEDIKPRVLEIRETRSEVGDYGLGRR 240

Query: 241 SCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGTGF 300
           SCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGTGF
Sbjct: 241 SCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGTGF 300

Query: 301 EKDAPSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRSSSHRKGASGDFDD 360
           EKD PSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRS SHRKGASGDFDD
Sbjct: 301 EKDDPSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRSCSHRKGASGDFDD 360

Query: 361 LKLISNAKVSPATTELFYGAKVLITEKDLNNSRSKATRDGDLSGTDVTSKDSVPDAAVVD 420
           LKLISNAKVSPATTELFYGAKVLITEKDLNNS SKATR+GDLSGTDVTSKDSVPDAAV+D
Sbjct: 361 LKLISNAKVSPATTELFYGAKVLITEKDLNNSHSKATREGDLSGTDVTSKDSVPDAAVID 420

Query: 421 RKTFKKVHRWRKVLSVLGMIQKRSGESKSDDEESSVGGNVVDRPIAESWEKLRRVANGEA 480
           RKTFKKVHRWRKVLSVLGMIQKRSGESKSDDEESSVGGNVVDRPIAESWEKLRRVANGEA
Sbjct: 421 RKTFKKVHRWRKVLSVLGMIQKRSGESKSDDEESSVGGNVVDRPIAESWEKLRRVANGEA 480

Query: 481 NSCVSQKLIRSYSVSCRDPSKLAGFNGSNDSKLNVSRWRDDFTLQRNRSVRYSPNNFDNG 540
           NSCVSQKLIRSYSVSCRDPSKLAGFNGSNDSKLNVSRWRDDFTLQRNRSVRYSPNNFDNG
Sbjct: 481 NSCVSQKLIRSYSVSCRDPSKLAGFNGSNDSKLNVSRWRDDFTLQRNRSVRYSPNNFDNG 540

Query: 541 LLRFYLTPLRSYSSRGKPGKSRPRSSPFNVKHVM 575
           LLRFYLTPLRSYSSRGKPGKSRPR+SPFNVKHVM
Sbjct: 541 LLRFYLTPLRSYSSRGKPGKSRPRNSPFNVKHVM 574

BLAST of Lsi10G014150 vs. NCBI nr
Match: XP_008464059.1 (PREDICTED: UPF0503 protein At3g09070, chloroplastic [Cucumis melo] >KAA0061854.1 UPF0503 protein [Cucumis melo var. makuwa] >TYK15402.1 UPF0503 protein [Cucumis melo var. makuwa])

HSP 1 Score: 1056.2 bits (2730), Expect = 1.0e-304
Identity = 534/575 (92.87%), Postives = 551/575 (95.83%), Query Frame = 0

Query: 1   MNLQLKTVSHRLSTCHRHPSKPVTGFCASCLRERLAGIDPDTQQESPVRNNHFSS-ELRR 60
           MNLQLK+VSHRLSTCHRHPSKPVTGFCASCLRERLAGIDPD Q ESP+ NNH SS ELRR
Sbjct: 1   MNLQLKSVSHRLSTCHRHPSKPVTGFCASCLRERLAGIDPDLQHESPLPNNHSSSAELRR 60

Query: 61  SKSFSAAKCEAGIGQSEVQHRKSCDVRSGNSLSDLFCREDKPRCTNREVEIESENLGFEL 120
           SKS+SAAKCEAGIGQSE+QHRKSCDVRSGNSLSDLFCREDKPRCTN EVEIESENLGFEL
Sbjct: 61  SKSYSAAKCEAGIGQSELQHRKSCDVRSGNSLSDLFCREDKPRCTNPEVEIESENLGFEL 120

Query: 121 REVVANERQFRASEGIIGPALGTIDDFSGDEAEFKTVKEFIDIEFRRKKNAGRDLREIAG 180
           REVV N RQFRASEGIIGP LGTIDDF+G++AEFKTVKEFID+EFRRKKNAGRDLREIAG
Sbjct: 121 REVVGNGRQFRASEGIIGPGLGTIDDFAGEDAEFKTVKEFIDLEFRRKKNAGRDLREIAG 180

Query: 181 SVWEAASVFSKKLGKWRKKQKRKNLSNNSNVGAVKAEDIKPRELEIRETRSEVGEYGLGR 240
           SVWEAASVFSKKL KWRKKQKRKNL NNSNVGAVK EDIKPR LEIRETRSEVGEYGLGR
Sbjct: 181 SVWEAASVFSKKLSKWRKKQKRKNLGNNSNVGAVKVEDIKPRALEIRETRSEVGEYGLGR 240

Query: 241 RSCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGTG 300
           RSCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSG G
Sbjct: 241 RSCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGAG 300

Query: 301 FEKDAPSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRSSSHRKGASGDFD 360
           FEKD PSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRS SHRKGASGDFD
Sbjct: 301 FEKDDPSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRSCSHRKGASGDFD 360

Query: 361 DLKLISNAKVSPATTELFYGAKVLITEKDLNNSRSKATRDGDLSGTDVTSKDSVPDAAVV 420
           +LKLISNAKVSPATTELFYGAKVLITEKDLN+SR KAT DGDLSGTDVTSKDSVPDA V+
Sbjct: 361 ELKLISNAKVSPATTELFYGAKVLITEKDLNSSRPKATGDGDLSGTDVTSKDSVPDAPVI 420

Query: 421 DRKTFKKVHRWRKVLSVLGMIQKRSGESKSDDEESSVGGNVVDRPIAESWEKLRRVANGE 480
           DRK+FKKVHRWRKVLSVLGMIQKR+GESKSDDEESSV GNVVDRP+ ESWEKLRRVANGE
Sbjct: 421 DRKSFKKVHRWRKVLSVLGMIQKRNGESKSDDEESSVAGNVVDRPVVESWEKLRRVANGE 480

Query: 481 ANSCVSQKLIRSYSVSCRDPSKLAGFNGSNDSKLNVSRWRDDFTLQRNRSVRYSPNNFDN 540
           ANSCVSQKLIRSYSVSCRDPSKLAGFNG NDSKLNV+RWRDDFTLQRNRSVRYSPNNFDN
Sbjct: 481 ANSCVSQKLIRSYSVSCRDPSKLAGFNGGNDSKLNVTRWRDDFTLQRNRSVRYSPNNFDN 540

Query: 541 GLLRFYLTPLRSYSSRGKPGKSRPRSSPFNVKHVM 575
           GLLRFYLTPLRSY SRGK GKSRPR+SPFNVKHV+
Sbjct: 541 GLLRFYLTPLRSY-SRGKLGKSRPRNSPFNVKHVI 574

BLAST of Lsi10G014150 vs. NCBI nr
Match: XP_004143144.1 (protein OCTOPUS [Cucumis sativus] >KGN47105.1 hypothetical protein Csa_021055 [Cucumis sativus])

HSP 1 Score: 1054.7 bits (2726), Expect = 2.9e-304
Identity = 536/576 (93.06%), Postives = 553/576 (96.01%), Query Frame = 0

Query: 1   MNLQLKTVSHRLSTCHRHPSKPVTGFCASCLRERLAGIDPDTQQESPVRNNHFSSELRRS 60
           MNLQLK+VSHRLSTCHRHPSKPVTGFCASCLRERLAGIDPDTQ ESPV+NNH S+ELRRS
Sbjct: 1   MNLQLKSVSHRLSTCHRHPSKPVTGFCASCLRERLAGIDPDTQHESPVQNNHSSAELRRS 60

Query: 61  KSFSAAKCEA-GIGQSEVQHRKSCDVRSGNSLSDLFCREDKPRCTNREVEIESENLGFEL 120
           KS SAAKCEA GIGQSEVQHRKSCDVRSGNSLSDLFCREDKPRCTNREVEIESENLGFEL
Sbjct: 61  KSHSAAKCEAGGIGQSEVQHRKSCDVRSGNSLSDLFCREDKPRCTNREVEIESENLGFEL 120

Query: 121 REVVANERQFRASEGIIGPALGTIDDFSGDEAEFKTVKEFIDIEFRRKKNAGRDLREIAG 180
           REVV N RQFRASEGIIGP LGTID FSG+EAEFKTVKEFID+EFRRKKNAGRDLREIAG
Sbjct: 121 REVVGNGRQFRASEGIIGPGLGTIDGFSGEEAEFKTVKEFIDLEFRRKKNAGRDLREIAG 180

Query: 181 SVWEAASVFSKKLGKWRKKQKRKNLSNNSNVGAVKAEDIKPRELEIRETRSEVGEYGLGR 240
           SVWEAAS FSKKLGKWRKKQKRKNLSNNS VGAVKAEDIKPR  EIRETRSEVGEYGLGR
Sbjct: 181 SVWEAASGFSKKLGKWRKKQKRKNLSNNSYVGAVKAEDIKPRAHEIRETRSEVGEYGLGR 240

Query: 241 RSCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGTG 300
           RSCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGTG
Sbjct: 241 RSCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGTG 300

Query: 301 FEKDAPSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRSSSHRKGASGDFD 360
           FEKD PSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSS+RRRKSFDRS SHRKGASGDFD
Sbjct: 301 FEKDDPSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSSMRRRKSFDRSCSHRKGASGDFD 360

Query: 361 DLKLISNAKVSPATTELFYGAKVLITEKDLNNSRSKATRDGDLSGTDVTSKDSVPDAAVV 420
           +LKLISNAKVSPATTELFYGAKVLITEKDLN+SR K T DGDLSGTDVTSKDSVPDA V+
Sbjct: 361 ELKLISNAKVSPATTELFYGAKVLITEKDLNSSRPKTTGDGDLSGTDVTSKDSVPDAPVI 420

Query: 421 DRKTFKKVHRWRKVLSVLGMIQKRSGESKSDDEESSVGGNVVDRPIAESWEKLRRVANGE 480
           DRKTFKKVHRWRKVLSVLGM+QKR+GESKSDDEESSVGGNVVDRP+ ESWEKLRRVANGE
Sbjct: 421 DRKTFKKVHRWRKVLSVLGMVQKRNGESKSDDEESSVGGNVVDRPVVESWEKLRRVANGE 480

Query: 481 ANSCVSQKLIRSYSVSCRDPSKLAGFNGSNDSKLNVSRWRDDFTLQRNRSVRYSPNNFDN 540
           ANSCVSQKLIRSYSVSCRDPSKLAGFNG NDSKLNV+RWRDDFTLQRNRSVRYSPNNFDN
Sbjct: 481 ANSCVSQKLIRSYSVSCRDPSKLAGFNGGNDSKLNVTRWRDDFTLQRNRSVRYSPNNFDN 540

Query: 541 -GLLRFYLTPLRSYSSRGKPGKSRPRSSPFNVKHVM 575
            GLLRFYLTPLRSY +RGK GK+RPR+SPFNVKHV+
Sbjct: 541 GGLLRFYLTPLRSY-NRGKLGKNRPRNSPFNVKHVI 575

BLAST of Lsi10G014150 vs. NCBI nr
Match: KAG6605428.1 (Protein OCTOPUS, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 984.9 bits (2545), Expect = 2.8e-283
Identity = 507/575 (88.17%), Postives = 532/575 (92.52%), Query Frame = 0

Query: 1   MNLQLKTVSHRLSTCHRHPSKPVTGFCASCLRERLAGIDPDTQQESPVRNNHFSSELRRS 60
           MNLQ K VSHRLS+C RHPS+PVTGFCASCLRERLAGID DT+QESPV N H +SELRRS
Sbjct: 1   MNLQNKAVSHRLSSCRRHPSEPVTGFCASCLRERLAGIDSDTRQESPVLNQHSTSELRRS 60

Query: 61  KSFSAAKCEAGIGQSEVQHRKSCDVRSGNSLSDLFCREDKPRCTNREVEIESENLGFELR 120
           KSFSAAK EAGIG+ EVQHRKSCD RSGNSLSDLFCREDKPRCTNREVEIESENLGFELR
Sbjct: 61  KSFSAAKREAGIGRPEVQHRKSCDARSGNSLSDLFCREDKPRCTNREVEIESENLGFELR 120

Query: 121 EVVANERQFRASEGIIGPALGTIDDFSGDEAEFKTVKEFIDIEFRRKKNAGRDLREIAGS 180
           EVVA ERQFRASEG+IGPAL  IDDFSG++AEFKT+KEFID+EFRRKKNAGRDLREIAGS
Sbjct: 121 EVVAKERQFRASEGVIGPALDIIDDFSGEDAEFKTMKEFIDLEFRRKKNAGRDLREIAGS 180

Query: 181 VWEAASVFSKKLGKWRKKQKRKNLSNNSNVGAVKAEDIKPRELEIRETRSEVGEYGLGRR 240
           VWEAASVFSKKLGKWRKKQK KNL N+++VGA K E IKPR LE+RETRSEVGEYGLGRR
Sbjct: 181 VWEAASVFSKKLGKWRKKQKMKNLGNDTDVGAAKTEAIKPRVLEVRETRSEVGEYGLGRR 240

Query: 241 SCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGTGF 300
           SCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIG+T+PR TPMVSVLEE K  G GF
Sbjct: 241 SCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGRTHPRPTPMVSVLEETKLPGIGF 300

Query: 301 EKDAPSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRSSSHRKGASGDFDD 360
           EKD P DEAEGSPMNVGDKIPGGSAQTKDYYMDSLS++RRRKSFDRSSSH+KGAS DFDD
Sbjct: 301 EKDDPYDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSTVRRRKSFDRSSSHKKGASADFDD 360

Query: 361 LKLISNAKVSPATTELFYGAKVLITEKDLNNSRSKATRDGDLSGTDVTSKDSVPDAAVVD 420
           LKLISNAKVSPATTELFYGAKVLITEKDL +S SKATRDGDLSGT++TSKDSVPDAA +D
Sbjct: 361 LKLISNAKVSPATTELFYGAKVLITEKDLKDSHSKATRDGDLSGTNITSKDSVPDAAGID 420

Query: 421 RKTFKKVHRWRKVLSVLGMIQKRSGE-SKSDDEESSVGGNVVDRPI-AESWEKLRRVANG 480
           RKTFKKVHRWRKVLSVLGM+QKRSGE SKSDDEES VGGNVVDRP  AESWEKLRRVANG
Sbjct: 421 RKTFKKVHRWRKVLSVLGMLQKRSGESSKSDDEESCVGGNVVDRPFAAESWEKLRRVANG 480

Query: 481 EANSCVSQKLIRSYSVSCRDPSKLAGFN-GSNDSKLNVSRWRDDFTLQRNRSVRYSPNNF 540
           EAN  VSQKLIRSYSVSCRDPSKLAGFN G NDSKL   R RDDFTLQRNRSVRYSPNNF
Sbjct: 481 EANGSVSQKLIRSYSVSCRDPSKLAGFNGGGNDSKLYGLRRRDDFTLQRNRSVRYSPNNF 540

Query: 541 DNGLLRFYLTPLRSYSSRGKPGKSRPRSSPFNVKH 573
           DNGLLRFYLTPLRSY +RGKPGKSRPRSSPFNVKH
Sbjct: 541 DNGLLRFYLTPLRSY-NRGKPGKSRPRSSPFNVKH 574

BLAST of Lsi10G014150 vs. NCBI nr
Match: XP_022948149.1 (UPF0503 protein At3g09070, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 984.2 bits (2543), Expect = 4.8e-283
Identity = 506/575 (88.00%), Postives = 532/575 (92.52%), Query Frame = 0

Query: 1   MNLQLKTVSHRLSTCHRHPSKPVTGFCASCLRERLAGIDPDTQQESPVRNNHFSSELRRS 60
           MNLQ K VSHRLS+C RHPS+PVTGFCASCLRERLAGID DT+QESPV N H +SELRRS
Sbjct: 1   MNLQNKAVSHRLSSCRRHPSEPVTGFCASCLRERLAGIDSDTRQESPVLNQHSTSELRRS 60

Query: 61  KSFSAAKCEAGIGQSEVQHRKSCDVRSGNSLSDLFCREDKPRCTNREVEIESENLGFELR 120
           KSFSAAK EAGIG+ EVQHRKSCD RSGNSLSDLFCREDKPRCTNREVEIESENLGFELR
Sbjct: 61  KSFSAAKREAGIGRPEVQHRKSCDARSGNSLSDLFCREDKPRCTNREVEIESENLGFELR 120

Query: 121 EVVANERQFRASEGIIGPALGTIDDFSGDEAEFKTVKEFIDIEFRRKKNAGRDLREIAGS 180
           EVVA ERQFRASEG+IGPAL  IDDFSG++AEFKT+KEFID+EFRRKKNAGRDLREIAGS
Sbjct: 121 EVVAKERQFRASEGVIGPALDIIDDFSGEDAEFKTMKEFIDLEFRRKKNAGRDLREIAGS 180

Query: 181 VWEAASVFSKKLGKWRKKQKRKNLSNNSNVGAVKAEDIKPRELEIRETRSEVGEYGLGRR 240
           VWEAASVFSKKLGKWRKKQK KNL N+++VGA K E IKPR LE+RETRSEVGEYGLGRR
Sbjct: 181 VWEAASVFSKKLGKWRKKQKMKNLGNDTDVGAAKTEAIKPRVLEVRETRSEVGEYGLGRR 240

Query: 241 SCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGTGF 300
           SCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIG+T+PR TPMVSVLEE K  G GF
Sbjct: 241 SCDTDPRFSVDAGRMSLDDSRYSFDEPRASWDGYLIGRTHPRPTPMVSVLEETKLPGIGF 300

Query: 301 EKDAPSDEAEGSPMNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRSSSHRKGASGDFDD 360
           EKD PSDEAEGSPMNVG+KIPGGSAQTKDYYMDSLS++RRRKSFDRSSSH+KGAS DFDD
Sbjct: 301 EKDDPSDEAEGSPMNVGEKIPGGSAQTKDYYMDSLSTVRRRKSFDRSSSHKKGASADFDD 360

Query: 361 LKLISNAKVSPATTELFYGAKVLITEKDLNNSRSKATRDGDLSGTDVTSKDSVPDAAVVD 420
           LKLISNAKVSPATTELFYGAKVLITEKDL +S SKATRDGDLSGT++TSKDSVPDAA +D
Sbjct: 361 LKLISNAKVSPATTELFYGAKVLITEKDLKDSHSKATRDGDLSGTNITSKDSVPDAAGID 420

Query: 421 RKTFKKVHRWRKVLSVLGMIQKRSGE-SKSDDEESSVGGNVVDRPI-AESWEKLRRVANG 480
           RKTFKKVHRWRKVLSVLGM+QKRSGE SKSDDEES VGGNVVDRP  AESWEKLRRVANG
Sbjct: 421 RKTFKKVHRWRKVLSVLGMLQKRSGESSKSDDEESCVGGNVVDRPFAAESWEKLRRVANG 480

Query: 481 EANSCVSQKLIRSYSVSCRDPSKLAGFN-GSNDSKLNVSRWRDDFTLQRNRSVRYSPNNF 540
           EAN  VSQKLIRSYSVSCRDPSKLAGFN G NDSKL   R RDDFTLQRNRS RYSPNNF
Sbjct: 481 EANGSVSQKLIRSYSVSCRDPSKLAGFNGGGNDSKLYGLRRRDDFTLQRNRSARYSPNNF 540

Query: 541 DNGLLRFYLTPLRSYSSRGKPGKSRPRSSPFNVKH 573
           DNGLLRFYLTPLRSY +RGKPGKSRPRSSPFNVKH
Sbjct: 541 DNGLLRFYLTPLRSY-NRGKPGKSRPRSSPFNVKH 574

BLAST of Lsi10G014150 vs. TAIR 10
Match: AT5G58930.1 (Protein of unknown function (DUF740) )

HSP 1 Score: 262.3 bits (669), Expect = 9.1e-70
Identity = 211/593 (35.58%), Postives = 297/593 (50.08%), Query Frame = 0

Query: 13  STCHRHP-SKPVTGFCASCLRERLAGIDPDTQQESPVRNNHFSSELRRSKSFSAAKCEAG 72
           + CHRHP SKP TGFCA+CLRERL+ I+  +   S       S+ELRR +S+S     A 
Sbjct: 15  AVCHRHPSSKPTTGFCATCLRERLSTIEALSSSVSA------STELRRVRSYSVRDASAS 74

Query: 73  IGQSEVQHRKSCDVRSGNSLSDLFCREDKPRCTNREVEIESENLGFELREVVANERQFRA 132
           +   +   R+SCDVRS +   D             + E+   ++ F +   +  + +   
Sbjct: 75  V--LDQPRRRSCDVRSNHDDDD-------------DDELLKSSIRFPIVPDLIEDEEEED 134

Query: 133 SEGIIGPALGTIDDFSGDEAEFKTVKEFIDIEFRRK--KNAGRDLREIAGSVWEAASVFS 192
            EG        + +   ++ E KT+KE ID+E R +  KN G+D            SVFS
Sbjct: 135 DEG------KKLVEEEIEDGEQKTMKELIDLESRNQQLKNNGKD------------SVFS 194

Query: 193 KKLGKWRKKQKRKNLSNNSNVGAVKAEDIKPRELEIRETRSEVGEYGLGRRSCDTDPRFS 252
           + L K+  K  RK + ++ N                           LGRRSCD DPR S
Sbjct: 195 RTLRKFSLKHHRK-IPDSGN--------------------------SLGRRSCDVDPRLS 254

Query: 253 VDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGTGFEKDAPSDEA 312
           +DAGR+       SFDEPRASWDG LIGKTYP++ P+ SV E+ K S      +   ++ 
Sbjct: 255 LDAGRV-------SFDEPRASWDGCLIGKTYPKLIPLSSVTEDVKASPEKITGEKVEEDE 314

Query: 313 EGSPMNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRSSSHRKGASGDFDDLKLISNAKV 372
           + +        PGG+AQT+DYY+DS    RRR+SFDRSS H      + D+LK ISNAKV
Sbjct: 315 KNN--------PGGTAQTRDYYLDS----RRRRSFDRSSRH---GLLEVDELKAISNAKV 374

Query: 373 SPATTELFYGAKVLITEKDLNNSRSKATRDGDLSGTDVTSK--DSVPDAAVVDRKTF--- 432
           SP T  LF+GAK+L+TE++L +S   + ++      ++ SK    V    V  +  F   
Sbjct: 375 SPETVGLFHGAKLLVTERELRDSNWYSIKNYKPESLELGSKGVGCVAAGEVKKQDGFGLK 434

Query: 433 KKVHRWRKVLSVLGMIQKRSGESKSD---DEESSVGGNVVDRPIAESWEKLRRVANGEAN 492
           K    W K  +  G+IQ+++  +K++   ++   +GGN ++  +AES  KLRRVA GE N
Sbjct: 435 KSGKNWSKGWNFWGLIQRKTDVAKNEMKTEQSLKLGGNTMEGSLAESLLKLRRVAKGETN 494

Query: 493 SCVSQKLIRSYSVSC--------RDPSKLAGFNGSNDS---------------------- 552
             VS+KLIRSYSVS         R  S + GF G   S                      
Sbjct: 495 GDVSEKLIRSYSVSARKSCDGMLRGASIVNGFEGGRSSCDGLFHGSITGVETGRRSLCED 518

Query: 553 --KLNVSRWRDDFTLQRNRSVRYSPNNFDNGLLRFYLTPLRSYSSRGKPGKSR 563
                V   R+      ++   YSP+N  NG++RFYLTPL S+ +  K GKSR
Sbjct: 555 GMFHGVEGKRNHLLQSDDKLGTYSPDNLRNGMVRFYLTPLNSHMT-SKSGKSR 518

BLAST of Lsi10G014150 vs. TAIR 10
Match: AT2G38070.1 (Protein of unknown function (DUF740) )

HSP 1 Score: 251.5 bits (641), Expect = 1.6e-66
Identity = 225/638 (35.27%), Postives = 315/638 (49.37%), Query Frame = 0

Query: 10  HRLST-CHRHPSKPVTGFCASCLRERLAGIDPDTQQESPVRNN----------------- 69
           HR ST C RHP +  TGFC SCL +RL+ +D   +  + V ++                 
Sbjct: 32  HRPSTSCDRHPDERFTGFCPSCLFDRLSVLDITGKNNNAVASSSKKPPSSSAALKAIFKP 91

Query: 70  -----HFSSELRRSKSFSAAKCEA-GIGQSEVQHRKSCDVRSGNSLSDLFCREDKPRCTN 129
                 F  ELRR+KSFSA+K EA  +G  E Q R+SCDVR  N+L  LF  + +     
Sbjct: 92  SSSSGSFFPELRRTKSFSASKAEAFSLGAFEPQ-RRSCDVRVRNTLWSLFHEDAEHNSQT 151

Query: 130 RE------VEIESENLGFELREVVANERQFRASE---------GIIGPALGTIDDFSGDE 189
           +E       EI+ E +   ++  V  E     SE                  ID+   +E
Sbjct: 152 KEGLSVNCSEIDLERINSIVKSPVFEEETEIESEQDNEKDIKFETFKEPRSVIDEIVEEE 211

Query: 190 AEFKTVK-EFIDIEFRRK---KNAGRDLREIAGSVWEAASVFSKKLGKWRKKQK-RKNLS 249
            E +T K E   +EF  +   K   RD +EIAGS W AASVFSKKL KWR+KQK +K+ +
Sbjct: 212 EEEETKKVEDFTMEFNPQTTAKKTNRDFKEIAGSFWSAASVFSKKLQKWRQKQKLKKHRT 271

Query: 250 NNSNVGAVKAEDIKPRELEIRETRSEVGEYGLGRRSCDTDPRFSVDAGRMSL-------D 309
            N   G+      K    ++R+T+SE+ EYG GRRSCDTDPRFS+DAGR SL       D
Sbjct: 272 GNLGAGSSALPVEKAIGRQLRDTQSEIAEYGYGRRSCDTDPRFSIDAGRFSLDAGRVSVD 331

Query: 310 DSRYSFDEPRASWDGYLIGKTYP--RITPMVSVLEEAKFSGTGFEKDA--PSDEA-EGSP 369
           D RYSF+EPRASWDGYLIG+     R+  M+SV+E++         D   P +++ + S 
Sbjct: 332 DPRYSFEEPRASWDGYLIGRAAAPMRMPSMLSVVEDSPVRNHVHRSDTHIPVEKSPQVSE 391

Query: 370 MNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRSSSHRK---GASGDFDDLKLISNAKVS 429
             + + +PGGSAQT++YY+DS SS RRRKS DRSSS RK       + D+LKL  + +  
Sbjct: 392 AVIDEIVPGGSAQTREYYLDS-SSSRRRKSLDRSSSTRKLSASVMAEIDELKLTQDRE-- 451

Query: 430 PATTELFYGAKVLITEKDLNNSRSKATRDGDLS---GTDVTSKDSVPDAAVVDRKTFKKV 489
                    AK L+       S S + RD   S     ++  +++V       ++T K  
Sbjct: 452 ---------AKDLV-------SHSNSLRDDCCSVENNYEMGVRENVGTIECNKKRTKKSR 511

Query: 490 HRWRKVLSVLGMIQKRSGESKSDDEESSVGGNVVDRPIAESWEKLRRVANGEANSCVSQK 549
             W    ++ G++ +++G +K ++EE   G   VDR  + SW       N E  +    K
Sbjct: 512 WSW----NIFGLLHRKNG-NKYEEEERRSG---VDRTFSGSW-------NVEPRNGFDPK 571

Query: 550 LIRS-YSVSCRDPSKLAGFNGSNDSKLNVSRWRDDFTLQRNR-----SVRYSPNNFDNGL 575
           +IRS  SVS R      G                   LQRN      S +   +  +NG+
Sbjct: 572 MIRSNSSVSWRSSGTTGG------------------GLQRNSVDGYISGKKKVSKAENGM 616

BLAST of Lsi10G014150 vs. TAIR 10
Match: AT3G46990.1 (Protein of unknown function (DUF740) )

HSP 1 Score: 251.5 bits (641), Expect = 1.6e-66
Identity = 211/594 (35.52%), Postives = 309/594 (52.02%), Query Frame = 0

Query: 13  STCHRHPS-KPVTGFCASCLRERLAGIDPDTQQESPVRNNHFSSELRRSKSFSAAKCEAG 72
           S+CHRHPS KP +GFCASCLRERL  I+  +   + V+    + ELRR +S+S     A 
Sbjct: 14  SSCHRHPSAKPTSGFCASCLRERLVTIEAQSSSLAAVQ----TPELRRIRSYSVR--NAS 73

Query: 73  IGQSEVQHRKSCDVR-SGNSLSDLFCREDKPRCTNREVEIESENLGFELREVVANERQFR 132
           +  S+   R+SCDVR S +SL DLF  +D+ R  +   +    +L  E  E    E  + 
Sbjct: 74  VSVSDQPRRRSCDVRSSASSLLDLFVDDDEERVDSSIRKPLVPDLK-EEEEEEEEEEDYY 133

Query: 133 ASEGIIGPALGTIDDFSGDEAEFKTVKEFIDIEFRR--KKNAGRDLREIAGSVWEAASVF 192
             E I G   G          E KT+KEFID+++R   KKN G+DL+EI       ASV 
Sbjct: 134 DGEDIKGFDEGKPRKI---VEENKTMKEFIDLDWRNQIKKNNGKDLKEI-------ASVL 193

Query: 193 SKKLGKWRKKQKRKNLSNNSNVGAVKAEDIKPRELEIRETRSEVGEYGLGRRSCDTDPRF 252
           S++L  +   ++    S++   G V                        GR S D DPR 
Sbjct: 194 SRRLKNFTLNKRNDEKSDSRFAGIVN-----------------------GRHSSDVDPRL 253

Query: 253 SVDAGRMSLDDSRYSFDEPRASWDGYLIGKTYPRITPMVSVLEEAKFSGTGFEKDAPSDE 312
           S D GR+       SF++PR+SWDG LI K+Y ++T + +V E+AK +  G E++   ++
Sbjct: 254 SFDGGRI-------SFEKPRSSWDGCLIEKSYHKLTTLSTVTEDAK-AKCGVEEEEVEEK 313

Query: 313 AEGSPMNVGDKIPGGSAQTKDYYMDSLSSLRRRKSFDRSSSHRKGASGDFDDLKLISNAK 372
                    +K PGG+ QTK+YY DS    RRR+SFDRS S ++    + D+L+ ISNAK
Sbjct: 314 ---------EKSPGGTVQTKNYYSDS----RRRRSFDRSVSIKRQGLLEVDELRGISNAK 373

Query: 373 VSPATTELFYGAKVLITEKDLNNSRSKATRDGDLSGTDVTSKDSVPDAAVVDRKTFKKVH 432
           VSP T  LF+GAK+L+TEK+L +S   + ++      ++ SK  +  AA  + K    V 
Sbjct: 374 VSPETVGLFHGAKLLVTEKELRDSNWYSIKNVKPESKELVSKGKICIAAGGEGKKQDSVE 433

Query: 433 ------RWRKVLSVLGMIQKRSGESKSD---DEESSVGGNVVDRPIAESWEKLRRVANGE 492
                 +W K  ++ G+IQ R  E+K++   ++   + GN V+  +AES  KLRRV  GE
Sbjct: 434 LKKPRKKWPKGWNIWGLIQ-RKNEAKNEIKTEQILKLEGNAVEGSLAESLLKLRRVGKGE 493

Query: 493 ANSCVSQKLIRSYSVSCR--------DPSKLAGFNG---------------------SND 552
            N  VS+KL++SYSVS R          + ++GF G                     S D
Sbjct: 494 TNVGVSEKLLKSYSVSARKSCDGVRSGANIVSGFEGGRSSCDGLFHGSINSVEAGRNSCD 544

Query: 553 SKLN-VSRWRDDFTLQRNRSV-RYSPNNFDNGLLRFYLTPLRSYSSRGKPGKSR 563
             +N +   ++   LQRN +V   S  N +  + RFYL+P++S+ +  K GKSR
Sbjct: 554 GLVNGIEGKQNHHLLQRNANVGTCSQENLEKSMFRFYLSPVKSHKT-SKSGKSR 544

BLAST of Lsi10G014150 vs. TAIR 10
Match: AT3G09070.1 (Protein of unknown function (DUF740) )

HSP 1 Score: 239.2 bits (609), Expect = 8.2e-63
Identity = 227/671 (33.83%), Postives = 306/671 (45.60%), Query Frame = 0

Query: 10  HRLST-CHRHPSKPVTGFCASCLRERLAGIDPDTQQES--------------------PV 69
           HRLST C+RHP +  TGFC SCL ERL+ +D      S                    P 
Sbjct: 25  HRLSTSCNRHPEERFTGFCPSCLCERLSVLDQTNNGGSSSSSKKPPTISAAALKALFKPS 84

Query: 70  RNN---------------HFSSELRRSKSFSAAKCEAGIGQSEVQHRKSCDVRSGNSLSD 129
            NN                F  ELRR+KSFSA+K   G        R+SCDVR  +SL +
Sbjct: 85  GNNGVGGVNTNGNGRVKPGFFPELRRTKSFSASKNNEGFSGVFEPQRRSCDVRLRSSLWN 144

Query: 130 LFCRED---------------KPRCT---------NREVEIESENLGFELRE----VVAN 189
           LF +++               +PR +         N E E ES++   E  E    V A 
Sbjct: 145 LFSQDEQRNLPSNVTGGEIDVEPRKSSVAEPVLEVNDEGEAESDDEELEEEEEEDYVEAG 204

Query: 190 ERQFRASEG-------------------IIGPALGTIDDFSGDEAEFKTVKEFIDIEFRR 249
           + +     G                    + P  G        E E K +K++ID++ + 
Sbjct: 205 DFEILNDSGELMREKSDEIVEVREEIEEAVKPTKGL------SEEELKPIKDYIDLDSQT 264

Query: 250 KKNAGRDLREIAGSVWEAASVFSKKLGKWRKKQKRKNLSNNSN--VGAVKAEDIKPRELE 309
           KK +      +  S W AASVFSKKL KWR+ QK K   N  +   G+ +    KP   +
Sbjct: 265 KKPS------VRRSFWSAASVFSKKLQKWRQNQKMKKRRNGGDHRPGSARLPVEKPIGRQ 324

Query: 310 IRETRSEVGEYGLGRRSCDTDPRFSVDA--------------GRMSLDDSRYSFDEPRAS 369
           +R+T+SE+ +YG GRRSCDTDPRFS+DA              GR+SLDD RYSFDEPRAS
Sbjct: 325 LRDTQSEIADYGYGRRSCDTDPRFSLDAGRFSLDAGRFSVDIGRISLDDPRYSFDEPRAS 384

Query: 370 WDGYLIGKTY-------PRITPMVSVLEEA----------KFSGTGFEKDAPSDEAEGSP 429
           WDG LIG+T        P    M+SV+E+A                 E+ AP        
Sbjct: 385 WDGSLIGRTMFPPAARAPPPPSMLSVVEDAPPPVHRHVTRADMQFPVEEPAPPPPVVNQT 444

Query: 430 MNVGDK--IPGGSAQTKDYYMDSLSSLRRRKSFDRSSSH-RKGAS---GDFDDLKLISNA 489
             V D   IPGGS QT+DYY D  SS RRRKS DRSSS  RK A+    D D+ KL    
Sbjct: 445 NGVSDPVIIPGGSIQTRDYYTD--SSSRRRKSLDRSSSSMRKTAAAVVADMDEPKL---- 504

Query: 490 KVSPATTELFYGAKVLITEKDLNNSRSKATRDGDLSGTDVTSKDSVPDAAVVDRKTFKKV 549
            VS A +   Y   +    +D NN   +   +G      +     + D  V      KK 
Sbjct: 505 SVSSAISIDAYSGSL----RDNNNYAVETADNGSFREPAM----MIGDRKVNSNDNNKKS 564

Query: 550 HRWRKVLSVLGMIQKRSGESKSDDEESS------VGGNVVDRPIAESWEKLRRVANGEAN 552
            RW K  S+LG+I ++S     ++EE        + G +V+R ++ESW +LR    G   
Sbjct: 565 RRWGK-WSILGLIYRKSVNKYEEEEEEEEDRYRRLNGGMVERSLSESWPELRNGGGGGG- 624

BLAST of Lsi10G014150 vs. TAIR 10
Match: AT5G01170.1 (Protein of unknown function (DUF740) )

HSP 1 Score: 215.3 bits (547), Expect = 1.3e-55
Identity = 203/602 (33.72%), Postives = 284/602 (47.18%), Query Frame = 0

Query: 10  HRLST-CHRHPSKPVTGFCASCLRERLAGID------PDTQQESP--------------- 69
           HRLST C  HP +  +GFC SCL +RL+ +D      P +    P               
Sbjct: 23  HRLSTSCDLHPEERFSGFCPSCLCDRLSVLDHNAAPPPSSSSRKPPSISAVSLKALFKPS 82

Query: 70  ---VRNNH--------FSSELRRSKSFSAAKCEAGIGQSEVQHRKSCDVRSGNSLSDLFC 129
                N++        F  ELRR+KSFSA   E   G  E Q R+SCDVR  +   +L  
Sbjct: 83  SSGTNNSNGNGRVRPGFFPELRRTKSFSAKNNEGFSGGFEPQ-RRSCDVRLRDDERNLPI 142

Query: 130 RE----DKPRCTNREVEIESENLGF-ELREVVANERQFRASEGIIGPALGTIDDFSGDEA 189
            E    DK     RE  +    L   E  E+  +E       G I     +  +   +E 
Sbjct: 143 NEAASVDKIEEEARESSVSEIVLEVTEEAEIEEDEENGEKDPGEIVEEKSS--EIGEEEE 202

Query: 190 EFKTVKEFIDIEFRRKKNAGRDLREIAGSVWEAASVFSKKLGKWRKKQKRKNLSNNSNVG 249
           E K +K+++D+  + KK +   +++ AGS + AASVFSKKL KW++KQK K   N    G
Sbjct: 203 ELKPMKDYMDLYSQTKKPS---VKDFAGSFFSAASVFSKKLQKWKQKQKVKKPRNGVGGG 262

Query: 250 AVKAEDIKPRELEIRETRSEVGEYGLGRRSCDTDPRFSVDA-------GRMSLDDSRYSF 309
                  +P+            E G+GRRS DTDPRFS+DA       GR+S+DDSRYS 
Sbjct: 263 -------RPQ-----------SEIGVGRRSSDTDPRFSLDAGRFSVDIGRISMDDSRYSL 322

Query: 310 DEPRASWDGYLIGKTYPRITP----MVSVLEEAKFSGTGFE-KDAPSDEAEGSPMNVGDK 369
           DEPRASWDG+LIG+T     P    M+SV+E A  + +  +   +PS +      +    
Sbjct: 323 DEPRASWDGHLIGRTTAARVPLPPSMLSVVENAPLNRSDMQIPSSPSIKPISGDSDPIII 382

Query: 370 IPGGSAQTKDYYMDSLSSLRRRKSFDRSSSHRKGASGDFDDLKLISNAKVSPATTELFYG 429
           IPGGS QT+DYY    SS RRRKS DRS+S RK  + + +D+K +SN+  +         
Sbjct: 383 IPGGSNQTRDYYTGPPSS-RRRKSLDRSNSIRKIVT-ELEDVKSVSNSTTT--------- 442

Query: 430 AKVLITEKDLNNSRSKATRDGDLSGTDVTSKDSVPDAAVVDRKTFKKVHRWRKVLSVLGM 489
               I    +  + +K  ++GD                       KK  RW K  S+LG 
Sbjct: 443 ----IDSNSMETAENKGNQNGD-----------------------KKSRRWGK-WSILGF 502

Query: 490 IQKRSGESKSDDEES-SVGGNVVDRPIAESWEKLRRVANGEANSCVSQKLIRSYSVSCRD 549
           I ++  + + +D  S S    +V+R ++ESW ++R   NGE       K+ RS S     
Sbjct: 503 IYRKGKDDEEEDRYSRSNSAGMVERSLSESWPEMR---NGEGG---GPKMRRSNS----- 535

Query: 550 PSKLAGFNGSNDSKLNVSRWRDDFTLQRNRSVRYSPNNFDNGLLRFYLTPL-RSYSSRGK 560
                          NVS         RN+S RYS  + +NG+LRFYLTP+ RS+ + G 
Sbjct: 563 ---------------NVSWRSSGGGSARNKSSRYSSKDGENGMLRFYLTPMRRSWKTSGG 535

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SS801.2e-6133.83Protein OCTOPUS OS=Arabidopsis thaliana OX=3702 GN=OPS PE=1 SV=1[more]
Q9LFB91.8e-5433.72Protein OCTOPUS-like OS=Arabidopsis thaliana OX=3702 GN=OPSL1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7V3J14.9e-30592.87UPF0503 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold571G0045... [more]
A0A1S3CKL04.9e-30592.87UPF0503 protein At3g09070, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103502039... [more]
A0A0A0KBP21.4e-30493.06Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G187980 PE=4 SV=1[more]
A0A6J1G9142.3e-28388.00UPF0503 protein At3g09070, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1FY884.4e-28287.63UPF0503 protein At3g09070, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
XP_038901013.10.0e+0095.99protein OCTOPUS [Benincasa hispida][more]
XP_008464059.11.0e-30492.87PREDICTED: UPF0503 protein At3g09070, chloroplastic [Cucumis melo] >KAA0061854.1... [more]
XP_004143144.12.9e-30493.06protein OCTOPUS [Cucumis sativus] >KGN47105.1 hypothetical protein Csa_021055 [C... [more]
KAG6605428.12.8e-28388.17Protein OCTOPUS, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022948149.14.8e-28388.00UPF0503 protein At3g09070, chloroplastic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G58930.19.1e-7035.58Protein of unknown function (DUF740) [more]
AT2G38070.11.6e-6635.27Protein of unknown function (DUF740) [more]
AT3G46990.11.6e-6635.52Protein of unknown function (DUF740) [more]
AT3G09070.18.2e-6333.83Protein of unknown function (DUF740) [more]
AT5G01170.11.3e-5533.72Protein of unknown function (DUF740) [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008004Protein OCTOPUS-likePFAMPF05340DUF740coord: 10..45
e-value: 4.8E-10
score: 38.8
coord: 149..551
e-value: 5.8E-127
score: 425.0
coord: 44..113
e-value: 1.4E-11
score: 43.8
IPR008004Protein OCTOPUS-likePANTHERPTHR31659PROTEIN: UPF0503-LIKE PROTEIN, PUTATIVE (DUF740)-RELATEDcoord: 5..573
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 553..574
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 298..326
NoneNo IPR availablePANTHERPTHR31659:SF0EMB|CAB61945.1coord: 5..573

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G014150.1Lsi10G014150.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005886 plasma membrane