Lsi10G013460 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G013460
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionVery-long-chain (3R)-3-hydroxyacyl-CoA dehydratase
Locationchr10: 17690631 .. 17694565 (-)
RNA-Seq ExpressionLsi10G013460
SyntenyLsi10G013460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGAGTCAAAAGAAACAAAACAAAACCCAAGTTAATTCCAATCCTATTTTCCTACTGCCCTTGTCCTAGATGTGATTTTTTCCCAATTCCCAAATTGCCGTCTCCAAGGGAAAAACCTGAGACGAGAACCCATCAAAACAGAAACTTTCTGTTTCCATTGCCAATTTGACGGTCAAGAAATAGGTTACTGTTCAAACTCCCAACAACAAAATGTCTCGTCTATCAAATTTCTATCTTCTAACTTACAATTCCCTCCAAGCTTTTGGCTGGTAATGCTATTTTTCTTTTGCTCTTTCAATTTTTCTGGCCTAAACTTTCTGTTGTTCTTCGTCAACATAGAACTTTGTCTCTTGTTGTCAGGATGGTTTCTCTTTACAGACTTGTGGGAGACTTTGCAGTCACTAAATCTACTACTGCTGCTTACTCTTCAGCAGGAGAACTTGTCTGTAAGTTTTTTGCTATCTGTTTTGTTCCTAGGGAAGTTGTGGAATCTTATGGGAAATGTCGGCTGAATTGGATTATGGATAAGACAAGTTCTTATGATTTCCTTTCTCTTCGAAGGTTTCCTGCAAACTGCTGCATTTTTGGAAATCATTCATGGAGCTGTTGGTAATTAAAGGAACTTTGGTGTTATTCTCTTGTTTAATGTCTAAATATGATAAAAGGGTTGTGATTTACCCTTTAAATTCCAGGCATTGTACCAACTGGAGTGCTGCTTCCTCTAATGCAATGGGGTGGGAGGGTTCATTTTGTGGTTGCAGTTGTTCGTCAACTTGATGAGGTTCTCTGTCTTTCTCCTTTTCTTGCACTTTCTTTACCAGAAAGATGCAGTTTCTTGTTGGATGTCTTCCTCCTAGTGCCAATGTTGATATAATACTAAATTTACTTTCATCTATCAGCTTAACATAGTATTAGAGCAAGATCATGTGTTCAAACCTCAGCAATGATATTTCCTTCCTAATTCATATTGATTTCACTTGTTGGGTCTTCTACATATTTTAAACCCACAAGTAAGGAGAGTATTCATATAATATTAAATTTGGGACAGTTGCAAATGTAGCAATTAGATTCAAAGTATTAGCATATATAGCAACATTTTTAAAAGATTGCAAATCGCTGATAGACTTTTATGGTCTAGGTAGTCTATCACTGATAGACCATAAGTCTATCGGCGATGGAAGTCTATCACTGATAGACTTTGCTATATTTGCAATTTTTTAAAAATATTGTTGTTCACTTAGTTATTATCCCTAAAATTGCTACTCTACAATTACCCATTAAATTTACCTTCACCTATTAGCTTAAGGAAGGTCATTCGGTGATTTAAAAAAAAAAAAAAAAAAAAAAATAGTAGAAAAATTGTCTACTCAATTGTATAATTAATGAAGAGCAAACTTAGGTATATATAAGCATAAGTGCTCCACTAACTACTCTAACAAACTATTTAACAGTGTCGATTACAAACTAAATAATATAAACATCCATAAAAATCATAACTAATACAAGGACATCACAATTCTTTCTTTTTGTTTGATAGATTCAACAGTTGCCTTCTGTTTTCATCACTTTCCTGGCTTGGAGCTTATCTGAGGTAAATTCTTATCTTTTTCCTTCAAAATTAGGTACCTTTGGCTTTTGTTCATTTCATTCTGATTTCTTTAGATTTTATTGATTGTCAGAGACTAATAGCCAAAATATCAGAGATTTGACCTTGCTCTACTAAAATCTAGATAAAGTAGACATTAATTAAACCTTAATGGAAGACGTCATTTAAGAAACCCTTTATGGTATTGGTTGCATTTTTTTATGATAAAGTGGACTTATTTATCAGAACTGAAAAGTGCAATCTAAGTCTTCTGAATGCAACCTATCTATATCTGAAAGAAGAAAATGAAGAAAGAAAAGGGTTAATGGCAATCTCAAAGTAAAATGACACTGATCTTTCTTATTGTACTTTGCTAGTTTGCTGTCTTCTAAACCATGTCCTTCTCTCTCTTTGCTGCTGATTTCCATTGGTTTACCAGGAGTTCCATCTCTGCTTCACATTGTAATTCCTTCATAGGTTTCCTTATTCAATTGGGTTTCTAGATTTTTACTGTGATGAAATGGATTCAGGTTATCAGATATTCTCATCATGCTTTGAATTGCTTGGGAGTATGCCCATTTTGGATTACATATCTTAGGTACTCTTCCTGAATGTTCTTCCAAGGAAAATATGTACTCAAAATGAGAAAAATAATTTTAATATTAAAGTATGTGTTCTTGCTTCTTCTAGGTACACTGCATTCATAGTTCTCTATCCTACTGGAGTGTCAATTGGTGAAAGTGAGTACACAATATCTAACTCATCTTCATTTCTGTAATTTGATATATCTCTAAGGGAGTGGCGGAGTGCTTAGTTCACAGGTCGGGTTTGTATAAGTCTAAAATTGTGGAGCCGAGCCATGTATTTTTACTTATTTCACTAAATCCTTCTGGCTACATAAACCAAGCTTCTAAATATATTCTCAAGCCATATACTTTTACAAATCACAAGCCTAAAAACCAAATATATATGCTACAAAAACCTTGGTTTCTAGAATTTATTCATCTACAAAATTTAAATACCGGTACATAAACTCAAGCTTCTCGACCCTAACTTACGCAAAACAAACCACCTTGTTTATTTCACCATGAGTCAAATGTCCCCTATTCTCTTTACCAATATTGTAAATTTACTTGTGTAATTTACTGTGAGTGATGCACAGAACAGAAACTAATATTCTATTTCATCAAGAAAAAGGATCATTGGCGACTTTTTAGAAGCATTTGATTTTGTGCAGTGTGGCTCATGTACCAAGCAATTCCATTTATGATAAAGAAGAACCTTTATGCAGATGTCTTTGCTTACCTCCCCTTCAGCTATTACAATTTCATCAGGGTATGTCCAAACCCTCAGTAGATCTTTCTAGGAAGAACTTGGTTATTTTGGTTTACCTTAAGTGAGATTACTCTAGATGTAATCCTGCCAATATATGCTTATCTTCCAATCTAATATTGTTGTTCAGGTTCTGCTTCTTGTCTACCCATTGTTGTTCTTGAAACTGTACCTACATCTGTTCAAGCAAAGGAGATCAAAATTGGGCAAGCAGCACAAGAAGAAGAGAAACTAAAGGTTAGGCTAATCTGGTATAATCTACAGTAAACAATTCATTCTTGGAAGTTTGATAATTTAGACAGTTAACGATCATGCTTGGATTTATTTATTTGTTTGACTTGATGGCATCACAATATATACACATATATATATATATATATTGAAACACGTCACAAAATTGATTTGGTGAATCACAAGATGGTGAATTGATTCTATAGCATCTAGTGCTAGCCAGTTAGTATGCAGATATAAAAAGGAAAGTTTTCAATGTTTGGGTTCAATGAGGCCTGTTAGTTTCCTATTGCAAAAAAAAATAATAATAATAAAATAAATAAAATAATTTGCTTTTTGACTGCTTAATCTTTTTGTTATGTCTATCATTATCTTTTCTTTTTTTTCCTTTCACAAATAGATATCATAAAAGCTCAATCTAGAATGAGGGAGGAGCCCTCGTTCAAGAATGATTTTTCCAGTGACCAGAATTATGGTGAGGAAAAGCAGAAGAATATAAATTTATGAGCCATAGATGATATTTTTCAGTACTGGTCGAATTATGTTGATTTTTTCCACGTTCAATTGACCTTTGTTTTTAGATATTACCAATAACCCACCATCAAGACCACTTCTAATTAACAATTGAGTGTGTGCTAGCTGACGCCAAAGCGGCTGAATTGAAATCTAATTGCTTATTAAATAAGATATTGTCTCAACAAGTAGTTGTTTAAATATTCTGTTACGTCAACAAGTAGTTGTCTTGTGTGTGTGTGTATATATATATATATTTTCTTGTTCACGGGTG

mRNA sequence

GTGAGTCAAAAGAAACAAAACAAAACCCAAGTTAATTCCAATCCTATTTTCCTACTGCCCTTGTCCTAGATGTGATTTTTTCCCAATTCCCAAATTGCCGTCTCCAAGGGAAAAACCTGAGACGAGAACCCATCAAAACAGAAACTTTCTGTTTCCATTGCCAATTTGACGGTCAAGAAATAGGTTACTGTTCAAACTCCCAACAACAAAATGTCTCGTCTATCAAATTTCTATCTTCTAACTTACAATTCCCTCCAAGCTTTTGGCTGGATGGTTTCTCTTTACAGACTTGTGGGAGACTTTGCAGTCACTAAATCTACTACTGCTGCTTACTCTTCAGCAGGAGAACTTGTCTGTTTCCTGCAAACTGCTGCATTTTTGGAAATCATTCATGGAGCTGTTGGCATTGTACCAACTGGAGTGCTGCTTCCTCTAATGCAATGGGGTGGGAGGGTTCATTTTGTGGTTGCAGTTGTTCGTCAACTTGATGAGATTCAACAGTTGCCTTCTGTTTTCATCACTTTCCTGGCTTGGAGCTTATCTGAGATTTTATTGATTGTTATCAGATATTCTCATCATGCTTTGAATTGCTTGGGAGTATGCCCATTTTGGATTACATATCTTAGTGTGGCTCATGTACCAAGCAATTCCATTTATGATAAAGAAGAACCTTTATGCAGATGTCTTTGCTTACCTCCCCTTCAGCTATTACAATTTCATCAGGGTTCTGCTTCTTGTCTACCCATTGTTGTTCTTGAAACTGTACCTACATCTGTTCAAGCAAAGGAGATCAAAATTGGGCAAGCAGCACAAGAAGAAGAGAAACTAAAGGTTAGGCTAATCTGGTATAATCTACAGTAAACAATTCATTCTTGGAAGTTTGATAATTTAGACAGTTAACGATCATGCTTGGATTTATTTATTTGTTTGACTTGATGGCATCACAATATATACACATATATATATATATATATTGAAACACGTCACAAAATTGATTTGGTGAATCACAAGATGGTGAATTGATTCTATAGCATCTAGTGCTAGCCAGTTAGTATGCAGATATAAAAAGGAAAGTTTTCAATGTTTGGGTTCAATGAGGCCTGTTAGTTTCCTATTGCAAAAAAAAATAATAATAATAAAATAAATAAAATAATTTGCTTTTTGACTGCTTAATCTTTTTGTTATGTCTATCATTATCTTTTCTTTTTTTTCCTTTCACAAATAGATATCATAAAAGCTCAATCTAGAATGAGGGAGGAGCCCTCGTTCAAGAATGATTTTTCCAGTGACCAGAATTATGGTGAGGAAAAGCAGAAGAATATAAATTTATGAGCCATAGATGATATTTTTCAGTACTGGTCGAATTATGTTGATTTTTTCCACGTTCAATTGACCTTTGTTTTTAGATATTACCAATAACCCACCATCAAGACCACTTCTAATTAACAATTGAGTGTGTGCTAGCTGACGCCAAAGCGGCTGAATTGAAATCTAATTGCTTATTAAATAAGATATTGTCTCAACAAGTAGTTGTTTAAATATTCTGTTACGTCAACAAGTAGTTGTCTTGTGTGTGTGTGTATATATATATATATTTTCTTGTTCACGGGTG

Coding sequence (CDS)

ATGTCTCGTCTATCAAATTTCTATCTTCTAACTTACAATTCCCTCCAAGCTTTTGGCTGGATGGTTTCTCTTTACAGACTTGTGGGAGACTTTGCAGTCACTAAATCTACTACTGCTGCTTACTCTTCAGCAGGAGAACTTGTCTGTTTCCTGCAAACTGCTGCATTTTTGGAAATCATTCATGGAGCTGTTGGCATTGTACCAACTGGAGTGCTGCTTCCTCTAATGCAATGGGGTGGGAGGGTTCATTTTGTGGTTGCAGTTGTTCGTCAACTTGATGAGATTCAACAGTTGCCTTCTGTTTTCATCACTTTCCTGGCTTGGAGCTTATCTGAGATTTTATTGATTGTTATCAGATATTCTCATCATGCTTTGAATTGCTTGGGAGTATGCCCATTTTGGATTACATATCTTAGTGTGGCTCATGTACCAAGCAATTCCATTTATGATAAAGAAGAACCTTTATGCAGATGTCTTTGCTTACCTCCCCTTCAGCTATTACAATTTCATCAGGGTTCTGCTTCTTGTCTACCCATTGTTGTTCTTGAAACTGTACCTACATCTGTTCAAGCAAAGGAGATCAAAATTGGGCAAGCAGCACAAGAAGAAGAGAAACTAAAGGTTAGGCTAATCTGGTATAATCTACAGTAA

Protein sequence

MSRLSNFYLLTYNSLQAFGWMVSLYRLVGDFAVTKSTTAAYSSAGELVCFLQTAAFLEIIHGAVGIVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSEILLIVIRYSHHALNCLGVCPFWITYLSVAHVPSNSIYDKEEPLCRCLCLPPLQLLQFHQGSASCLPIVVLETVPTSVQAKEIKIGQAAQEEEKLKVRLIWYNLQ
Homology
[Close]
BLAST of Lsi10G013460 vs. ExPASy Swiss-Prot
Match: Q2KIP8 (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 OS=Bos taurus OX=9913 GN=HACD2 PE=2 SV=2)

HSP 1 Score: 77.0 bits (188), Expect = 2.9e-13
Identity = 48/134 (35.82%), Postives = 73/134 (54.48%), Query Frame = 0 

Query: 4   LSNFYLLTYNSLQAFGWMVSLYRLVGDFAVTKSTTAAYSSAGELVCFLQTAAFLEIIHGA 63
           L+  YL+ YN +   GW+V    LV  +    S  + Y S  + + F QT A LEI+H A
Sbjct: 37  LTTAYLVIYNVVMTAGWLVIAVGLVRAYLAKGSYHSLYYSIEKPLKFFQTGALLEILHCA 96

Query: 64  VGIVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSEILLIVIRYSHH 123
           +GIVP+ V+L  +Q   RV  + AV   + E+Q   SV +  +AW+++EI    IRYS +
Sbjct: 97  IGIVPSSVVLTSIQVMSRVFLIWAVTHSVKEVQSEDSVLLFVIAWTITEI----IRYSFY 156

Query: 124 ALNCLGVCPFWITY 138
             + L   P+ I +
Sbjct: 157 TFSLLNHLPYLIKW 166

[Close]
BLAST of Lsi10G013460 vs. ExPASy Swiss-Prot
Match: Q6Y1H2 (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 OS=Homo sapiens OX=9606 GN=HACD2 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.7e-13
Identity = 48/134 (35.82%), Postives = 72/134 (53.73%), Query Frame = 0 

Query: 4   LSNFYLLTYNSLQAFGWMVSLYRLVGDFAVTKSTTAAYSSAGELVCFLQTAAFLEIIHGA 63
           L+  YL+ YN +   GW+V    LV  +    S  + Y S  + + F QT A LEI+H A
Sbjct: 37  LATAYLVIYNVVMTAGWLVIAVGLVRAYLAKGSYHSLYYSIEKPLKFFQTGALLEILHCA 96

Query: 64  VGIVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSEILLIVIRYSHH 123
           +GIVP+ V+L   Q   RV  + AV   + E+Q   SV +  +AW+++EI    IRYS +
Sbjct: 97  IGIVPSSVVLTSFQVMSRVFLIWAVTHSVKEVQSEDSVLLFVIAWTITEI----IRYSFY 156

Query: 124 ALNCLGVCPFWITY 138
             + L   P+ I +
Sbjct: 157 TFSLLNHLPYLIKW 166

[Close]
BLAST of Lsi10G013460 vs. ExPASy Swiss-Prot
Match: Q5RBK3 (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 OS=Pongo abelii OX=9601 GN=HACD2 PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 3.7e-13
Identity = 48/134 (35.82%), Postives = 72/134 (53.73%), Query Frame = 0 

Query: 4   LSNFYLLTYNSLQAFGWMVSLYRLVGDFAVTKSTTAAYSSAGELVCFLQTAAFLEIIHGA 63
           L+  YL+ YN +   GW+V    LV  +    S  + Y S  + + F QT A LEI+H A
Sbjct: 38  LATAYLVIYNVVMTAGWLVIAVGLVRAYLAKGSYHSLYYSIEKPLKFFQTGALLEILHCA 97

Query: 64  VGIVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSEILLIVIRYSHH 123
           +GIVP+ V+L   Q   RV  + AV   + E+Q   SV +  +AW+++EI    IRYS +
Sbjct: 98  IGIVPSSVVLTSFQVMSRVFLIWAVTHSVKEVQSEDSVLLFVIAWTITEI----IRYSFY 157

Query: 124 ALNCLGVCPFWITY 138
             + L   P+ I +
Sbjct: 158 TFSLLNHLPYLIKW 167

[Close]
BLAST of Lsi10G013460 vs. ExPASy Swiss-Prot
Match: Q9D3B1 (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 OS=Mus musculus OX=10090 GN=Hacd2 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-12
Identity = 47/130 (36.15%), Postives = 69/130 (53.08%), Query Frame = 0 

Query: 8   YLLTYNSLQAFGWMVSLYRLVGDFAVTKSTTAAYSSAGELVCFLQTAAFLEIIHGAVGIV 67
           YL+ YN +   GW+V    LV  +    S  + Y S    + F QT A LEI+H A+GIV
Sbjct: 41  YLVIYNVVMTAGWLVIAVGLVRAYLAKGSYHSLYYSIERPLKFFQTGALLEILHCAIGIV 100

Query: 68  PTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSEILLIVIRYSHHALNC 127
           P+ V+L   Q   RV  + AV   + E+Q   SV +  +AW+++EI    IRYS +  + 
Sbjct: 101 PSSVVLTSFQVMSRVFLIWAVTHSVKEVQSEDSVLLFVIAWTITEI----IRYSFYTFSL 160

Query: 128 LGVCPFWITY 138
           L   P+ I +
Sbjct: 161 LNHLPYIIKW 166

[Close]
BLAST of Lsi10G013460 vs. ExPASy Swiss-Prot
Match: Q9N1R5 (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1 OS=Ovis aries OX=9940 GN=HACD1 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.0e-10
Identity = 43/134 (32.09%), Postives = 67/134 (50.00%), Query Frame = 0 

Query: 4   LSNFYLLTYNSLQAFGWMVSLYRLVGDFAVTKSTTAAYSSAGELVCFLQTAAFLEIIHGA 63
           L+  +L  YN     GW+V    +V  +    +    Y S  + + F QT A LEI+H  
Sbjct: 71  LATIWLTFYNIAMTAGWLVLAIAMVRFYMEKGTHKGLYKSIQKTLKFFQTFALLEIVHCL 130

Query: 64  VGIVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSEILLIVIRYSHH 123
           +GIVPT VL+  +Q   R+  V  V   +  IQ   SV +  +AW+++EI     RYS +
Sbjct: 131 IGIVPTSVLVAGVQVSSRIFMVWLVTHSIKPIQNEESVVLFLVAWTVTEI----TRYSFY 190

Query: 124 ALNCLGVCPFWITY 138
             + L   P++I +
Sbjct: 191 TFSLLDHLPYFIKW 200

[Close]
BLAST of Lsi10G013460 vs. ExPASy TrEMBL
Match: A0A5D3DKD0 (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold250G00410 PE=3 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 1.0e-61
Identity = 123/138 (89.13%), Postives = 127/138 (92.03%), Query Frame = 0 

Query: 1   MSRLSNFYLLTYNSLQAFGWMVSLYRLVGDFAVTKSTTAAYSSAGELVCFLQTAAFLEII 60
           MSRLSN YLLTYNSLQ FGWMVSLYRLVGDF  TKST+ AYSS GELVCFLQTAAFLE+I
Sbjct: 1   MSRLSNLYLLTYNSLQGFGWMVSLYRLVGDFVATKSTSGAYSSTGELVCFLQTAAFLEVI 60

Query: 61  HGAVGIVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSEILLIVIRY 120
           HGAVG+VPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSE    VIRY
Sbjct: 61  HGAVGLVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSE----VIRY 120

Query: 121 SHHALNCLGVCPFWITYL 139
            HHALNCLGVCPFW+TYL
Sbjct: 121 FHHALNCLGVCPFWLTYL 134

[Close]
BLAST of Lsi10G013460 vs. ExPASy TrEMBL
Match: A0A0A0KG29 (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase OS=Cucumis sativus OX=3659 GN=Csa_6G303230 PE=3 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 1.0e-61
Identity = 123/138 (89.13%), Postives = 128/138 (92.75%), Query Frame = 0 

Query: 1   MSRLSNFYLLTYNSLQAFGWMVSLYRLVGDFAVTKSTTAAYSSAGELVCFLQTAAFLEII 60
           MSRLSN YLLTYNSLQ FGWMVSLYRLVGDFA TKST+ AYSS GELVCFLQTAAFLE+I
Sbjct: 1   MSRLSNLYLLTYNSLQGFGWMVSLYRLVGDFAATKSTSGAYSSTGELVCFLQTAAFLEVI 60

Query: 61  HGAVGIVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSEILLIVIRY 120
           HGAVG+VPTGVLLPLMQWGGRVHFVVAVVRQLDEIQ+LPSVFITFLAWSLSE    VIRY
Sbjct: 61  HGAVGLVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQRLPSVFITFLAWSLSE----VIRY 120

Query: 121 SHHALNCLGVCPFWITYL 139
            HHALNCLGVCPFW+TYL
Sbjct: 121 FHHALNCLGVCPFWLTYL 134

[Close]
BLAST of Lsi10G013460 vs. ExPASy TrEMBL
Match: A0A1S3BJI8 (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase OS=Cucumis melo OX=3656 GN=LOC103490580 PE=3 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 1.0e-61
Identity = 123/138 (89.13%), Postives = 127/138 (92.03%), Query Frame = 0 

Query: 1   MSRLSNFYLLTYNSLQAFGWMVSLYRLVGDFAVTKSTTAAYSSAGELVCFLQTAAFLEII 60
           MSRLSN YLLTYNSLQ FGWMVSLYRLVGDF  TKST+ AYSS GELVCFLQTAAFLE+I
Sbjct: 1   MSRLSNLYLLTYNSLQGFGWMVSLYRLVGDFVATKSTSGAYSSTGELVCFLQTAAFLEVI 60

Query: 61  HGAVGIVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSEILLIVIRY 120
           HGAVG+VPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSE    VIRY
Sbjct: 61  HGAVGLVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSE----VIRY 120

Query: 121 SHHALNCLGVCPFWITYL 139
            HHALNCLGVCPFW+TYL
Sbjct: 121 FHHALNCLGVCPFWLTYL 134

[Close]
BLAST of Lsi10G013460 vs. ExPASy TrEMBL
Match: A0A6J1D2Y7 (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase OS=Momordica charantia OX=3673 GN=LOC111017094 PE=3 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 6.8e-58
Identity = 115/138 (83.33%), Postives = 124/138 (89.86%), Query Frame = 0 

Query: 1   MSRLSNFYLLTYNSLQAFGWMVSLYRLVGDFAVTKSTTAAYSSAGELVCFLQTAAFLEII 60
           MSRLSN YLLTYNSLQAFGW +SLYRLVG FA TKST  AYSS GEL+CFLQTAAFLEII
Sbjct: 1   MSRLSNSYLLTYNSLQAFGWTISLYRLVGSFAATKSTIGAYSSVGELICFLQTAAFLEII 60

Query: 61  HGAVGIVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSEILLIVIRY 120
           HGAVG+VPTGVLLPLMQWGGRVHF++AVVR+L E+Q LP VFITF+AWSLSE    VIRY
Sbjct: 61  HGAVGLVPTGVLLPLMQWGGRVHFLIAVVRRLAEVQHLPYVFITFMAWSLSE----VIRY 120

Query: 121 SHHALNCLGVCPFWITYL 139
           SHHALNCLGVCPFW+TYL
Sbjct: 121 SHHALNCLGVCPFWLTYL 134

[Close]
BLAST of Lsi10G013460 vs. ExPASy TrEMBL
Match: A0A6J1G819 (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111451609 PE=3 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 1.2e-57
Identity = 116/138 (84.06%), Postives = 125/138 (90.58%), Query Frame = 0 

Query: 1   MSRLSNFYLLTYNSLQAFGWMVSLYRLVGDFAVTKSTTAAYSSAGELVCFLQTAAFLEII 60
           MSRLSNFYLLTYNSLQAFGW +SLYRL+  F  TKST+ AYSSAGEL+CFLQTAAFLEII
Sbjct: 1   MSRLSNFYLLTYNSLQAFGWTISLYRLLDSFVATKSTSGAYSSAGELICFLQTAAFLEII 60

Query: 61  HGAVGIVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSEILLIVIRY 120
           HGA+G+VPTGVLLPLMQWGGRVHF+VAVVRQL E+QQLPSVFITFLAWSLSE    VIRY
Sbjct: 61  HGAIGLVPTGVLLPLMQWGGRVHFLVAVVRQLHEVQQLPSVFITFLAWSLSE----VIRY 120

Query: 121 SHHALNCLGVCPFWITYL 139
           SHHALNCLGV  FW+TYL
Sbjct: 121 SHHALNCLGVSLFWLTYL 134

[Close]
BLAST of Lsi10G013460 vs. NCBI nr
Match: KAG7035426.1 (Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase hpo-8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 258.5 bits (659), Expect = 5.3e-65
Identity = 153/266 (57.52%), Postives = 169/266 (63.53%), Query Frame = 0 

Query: 1   MSRLSNFYLLTYNSLQAFGWMVSLYRLVGDFAVTKSTTAAYSSAGELVCFLQTAAFLEII 60
           MSRLSNFYLLTYNSLQAFGW +SLYRL+  F  TKST+ AYSSAGEL+CFLQTAAFLEII
Sbjct: 1   MSRLSNFYLLTYNSLQAFGWTISLYRLLDSFVATKSTSGAYSSAGELICFLQTAAFLEII 60

Query: 61  HGAVGIVP-------------TGVLLPLMQWGGRVHFVVAVVRQLDE------------- 120
           HGA+G +                V+   + +    ++ VA    +               
Sbjct: 61  HGAIGNLNELWYHSLVWYLNFDNVVFSPLNFRLGTNWSVASSNAMGRESSFSGCSCSSTS 120

Query: 121 -------IQQLPSVFITFLAWSLSEILLIVIRYSHHALNCLGVCPFWITYL--------- 180
                  +QQLPSVFITFLAWSLSE    VIRYSHHALNCLGV  FW+TYL         
Sbjct: 121 RGFSPHFVQQLPSVFITFLAWSLSE----VIRYSHHALNCLGVSLFWLTYLRYTAFIILY 180

Query: 181 ----------------SVAHVPSNSIYDKEEPLCRCLCLPPLQLLQFHQGSASCLPIVVL 209
                           SVAHVPSNS YDKE+PLCRCL LP LQLLQ HQGSA CLP + L
Sbjct: 181 PIGVTIGESEYTKHLCSVAHVPSNSFYDKEQPLCRCLRLPSLQLLQCHQGSAFCLPTIFL 240

[Close]
BLAST of Lsi10G013460 vs. NCBI nr
Match: XP_038900764.1 (very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 [Benincasa hispida])

HSP 1 Score: 246.9 bits (629), Expect = 1.6e-61
Identity = 124/138 (89.86%), Postives = 128/138 (92.75%), Query Frame = 0 

Query: 1   MSRLSNFYLLTYNSLQAFGWMVSLYRLVGDFAVTKSTTAAYSSAGELVCFLQTAAFLEII 60
           MSRLSNFYLLTYNSLQA GWMVSLYRLVG+FA TKST+ AYSS GELVCFLQ AAFLEII
Sbjct: 1   MSRLSNFYLLTYNSLQALGWMVSLYRLVGNFAATKSTSGAYSSTGELVCFLQAAAFLEII 60

Query: 61  HGAVGIVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSEILLIVIRY 120
           HGAVG+VPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSE    VIRY
Sbjct: 61  HGAVGLVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSE----VIRY 120

Query: 121 SHHALNCLGVCPFWITYL 139
           SHHA NCLGVCPFW+TYL
Sbjct: 121 SHHAFNCLGVCPFWLTYL 134

[Close]
BLAST of Lsi10G013460 vs. NCBI nr
Match: XP_008448368.1 (PREDICTED: very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 isoform X1 [Cucumis melo] >KAA0061952.1 very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 isoform X1 [Cucumis melo var. makuwa] >TYK23978.1 very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 246.5 bits (628), Expect = 2.1e-61
Identity = 123/138 (89.13%), Postives = 127/138 (92.03%), Query Frame = 0 

Query: 1   MSRLSNFYLLTYNSLQAFGWMVSLYRLVGDFAVTKSTTAAYSSAGELVCFLQTAAFLEII 60
           MSRLSN YLLTYNSLQ FGWMVSLYRLVGDF  TKST+ AYSS GELVCFLQTAAFLE+I
Sbjct: 1   MSRLSNLYLLTYNSLQGFGWMVSLYRLVGDFVATKSTSGAYSSTGELVCFLQTAAFLEVI 60

Query: 61  HGAVGIVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSEILLIVIRY 120
           HGAVG+VPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSE    VIRY
Sbjct: 61  HGAVGLVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSE----VIRY 120

Query: 121 SHHALNCLGVCPFWITYL 139
            HHALNCLGVCPFW+TYL
Sbjct: 121 FHHALNCLGVCPFWLTYL 134

[Close]
BLAST of Lsi10G013460 vs. NCBI nr
Match: XP_004148217.1 (very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 [Cucumis sativus] >KGN47357.1 hypothetical protein Csa_023054 [Cucumis sativus])

HSP 1 Score: 246.5 bits (628), Expect = 2.1e-61
Identity = 123/138 (89.13%), Postives = 128/138 (92.75%), Query Frame = 0 

Query: 1   MSRLSNFYLLTYNSLQAFGWMVSLYRLVGDFAVTKSTTAAYSSAGELVCFLQTAAFLEII 60
           MSRLSN YLLTYNSLQ FGWMVSLYRLVGDFA TKST+ AYSS GELVCFLQTAAFLE+I
Sbjct: 1   MSRLSNLYLLTYNSLQGFGWMVSLYRLVGDFAATKSTSGAYSSTGELVCFLQTAAFLEVI 60

Query: 61  HGAVGIVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSEILLIVIRY 120
           HGAVG+VPTGVLLPLMQWGGRVHFVVAVVRQLDEIQ+LPSVFITFLAWSLSE    VIRY
Sbjct: 61  HGAVGLVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQRLPSVFITFLAWSLSE----VIRY 120

Query: 121 SHHALNCLGVCPFWITYL 139
            HHALNCLGVCPFW+TYL
Sbjct: 121 FHHALNCLGVCPFWLTYL 134

[Close]
BLAST of Lsi10G013460 vs. NCBI nr
Match: XP_022148455.1 (very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 [Momordica charantia])

HSP 1 Score: 233.8 bits (595), Expect = 1.4e-57
Identity = 115/138 (83.33%), Postives = 124/138 (89.86%), Query Frame = 0 

Query: 1   MSRLSNFYLLTYNSLQAFGWMVSLYRLVGDFAVTKSTTAAYSSAGELVCFLQTAAFLEII 60
           MSRLSN YLLTYNSLQAFGW +SLYRLVG FA TKST  AYSS GEL+CFLQTAAFLEII
Sbjct: 1   MSRLSNSYLLTYNSLQAFGWTISLYRLVGSFAATKSTIGAYSSVGELICFLQTAAFLEII 60

Query: 61  HGAVGIVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSEILLIVIRY 120
           HGAVG+VPTGVLLPLMQWGGRVHF++AVVR+L E+Q LP VFITF+AWSLSE    VIRY
Sbjct: 61  HGAVGLVPTGVLLPLMQWGGRVHFLIAVVRRLAEVQHLPYVFITFMAWSLSE----VIRY 120

Query: 121 SHHALNCLGVCPFWITYL 139
           SHHALNCLGVCPFW+TYL
Sbjct: 121 SHHALNCLGVCPFWLTYL 134

[Close]
BLAST of Lsi10G013460 vs. TAIR 10
Match: AT5G59770.1 (Protein-tyrosine phosphatase-like, PTPLA )

HSP 1 Score: 127.5 bits (319), Expect = 1.3e-29
Identity = 59/117 (50.43%), Postives = 82/117 (70.09%), Query Frame = 0 

Query: 22  VSLYRLVGDFAVTKSTTAAYSSAGELVCFLQTAAFLEIIHGAVGIVPTGVLLPLMQWGGR 81
           +SL  ++  F   K+  +AY+SAG L+   QTAA LE++HGA+GIVP+G L PLMQW GR
Sbjct: 73  ISLLIILNSFLSNKTIISAYASAGFLISLFQTAAILEVLHGAIGIVPSGFLSPLMQWSGR 132

Query: 82  VHFVVAVVRQLDEIQQLPSVFITFLAWSLSEILLIVIRYSHHALNCLGVCPFWITYL 139
            HF++A+V Q+ E+Q  P + IT +AW + E    +IRY H+A  CLG CP+W+TYL
Sbjct: 133 THFILAIVGQIKEVQDSPWLSITLVAWCIGE----MIRYPHYAFTCLGRCPYWLTYL 185

[Close]
BLAST of Lsi10G013460 vs. TAIR 10
Match: AT5G10480.1 (Protein-tyrosine phosphatase-like, PTPLA )

HSP 1 Score: 54.7 bits (130), Expect = 1.1e-07
Identity = 47/177 (26.55%), Postives = 73/177 (41.24%), Query Frame = 0 

Query: 1   MSRLSNFYLLTYNSLQAFGWMVSLYRLVGDFAVTKSTTAAYSSAGELVCFLQTAAFLEII 60
           +S +   YL  YN +   GW   LY  +     T      Y +  + +   QTAA LEI+
Sbjct: 5   LSVVRRVYLTLYNWIVFAGWAQVLYLAITTLKET-GYENVYDAIEKPLQLAQTAAVLEIL 64

Query: 61  HGAVGIVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSEILLIVIRY 120
           HG VG+V + V   L Q G R+     ++    E++    V    ++WS++EI    IRY
Sbjct: 65  HGLVGLVRSPVSATLPQIGSRLFLTWGILYSFPEVRSHFLVTSLVISWSITEI----IRY 124

Query: 121 SHHAL-NCLGVCPFW----------------------ITYLSVAHVPSNSIYDKEEP 155
           S       LG  P W                      + YL++ H+ ++ +Y    P
Sbjct: 125 SFFGFKEALGFAPSWHLWLRYSSFLLLYPTGITSEVGLIYLALPHIKTSEMYSVRMP 176

[Close]
BLAST of Lsi10G013460 vs. TAIR 10
Match: AT5G10480.3 (Protein-tyrosine phosphatase-like, PTPLA )

HSP 1 Score: 52.8 bits (125), Expect = 4.1e-07
Identity = 46/182 (25.27%), Postives = 75/182 (41.21%), Query Frame = 0 

Query: 1   MSRLSNFYLLTYNSLQAFGWMVSLYRLVGDFAVTKSTTAAYSSAGELVCFLQTAAFLEII 60
           +S +   YL  YN +   GW   LY  +     T      Y +  + +   QTAA LEI+
Sbjct: 5   LSVVRRVYLTLYNWIVFAGWAQVLYLAITTLKET-GYENVYDAIEKPLQLAQTAAVLEIL 64

Query: 61  HGAVGIVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLSEI-----LL 120
           HG VG+V + V   L Q G R+     ++    E++    V    ++WS++E      ++
Sbjct: 65  HGLVGLVRSPVSATLPQIGSRLFLTWGILYSFPEVRSHFLVTSLVISWSITEFPITTWIV 124

Query: 121 IVIRYSHHAL-NCLGVCPFW----------------------ITYLSVAHVPSNSIYDKE 155
            +IRYS       LG  P W                      + YL++ H+ ++ +Y   
Sbjct: 125 EIIRYSFFGFKEALGFAPSWHLWLRYSSFLLLYPTGITSEVGLIYLALPHIKTSEMYSVR 184

[Close]
BLAST of Lsi10G013460 vs. TAIR 10
Match: AT5G10480.2 (Protein-tyrosine phosphatase-like, PTPLA )

HSP 1 Score: 49.3 bits (116), Expect = 4.5e-06
Identity = 36/126 (28.57%), Postives = 55/126 (43.65%), Query Frame = 0 

Query: 52  QTAAFLEIIHGAVGIVPTGVLLPLMQWGGRVHFVVAVVRQLDEIQQLPSVFITFLAWSLS 111
           QTAA LEI+HG VG+V + V   L Q G R+     ++    E++    V    ++WS++
Sbjct: 30  QTAAVLEILHGLVGLVRSPVSATLPQIGSRLFLTWGILYSFPEVRSHFLVTSLVISWSIT 89

Query: 112 EILLIVIRYSHHAL-NCLGVCPFW----------------------ITYLSVAHVPSNSI 155
           EI    IRYS       LG  P W                      + YL++ H+ ++ +
Sbjct: 90  EI----IRYSFFGFKEALGFAPSWHLWLRYSSFLLLYPTGITSEVGLIYLALPHIKTSEM 149

The following BLAST results are available for this feature:
BLAST of Lsi10G013460 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q2KIP82.9e-1335.82Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 OS=Bos taurus OX=9913 GN=HA... [more]
Q6Y1H23.7e-1335.82Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 OS=Homo sapiens OX=9606 GN=... [more]
Q5RBK33.7e-1335.82Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 OS=Pongo abelii OX=9601 GN=... [more]
Q9D3B11.1e-1236.15Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 OS=Mus musculus OX=10090 GN... [more]
Q9N1R53.0e-1032.09Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1 OS=Ovis aries OX=9940 GN=HA... [more]
BLAST of Lsi10G013460 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3DKD01.0e-6189.13Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase OS=Cucumis melo var. makuwa O... [more]
A0A0A0KG291.0e-6189.13Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase OS=Cucumis sativus OX=3659 GN... [more]
A0A1S3BJI81.0e-6189.13Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase OS=Cucumis melo OX=3656 GN=LO... [more]
A0A6J1D2Y76.8e-5883.33Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase OS=Momordica charantia OX=367... [more]
A0A6J1G8191.2e-5784.06Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase OS=Cucurbita moschata OX=3662... [more]
BLAST of Lsi10G013460 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7035426.15.3e-6557.52Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase hpo-8 [Cucurbita argyrosperma... [more]
XP_038900764.11.6e-6189.86very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 [Benincasa hispida][more]
XP_008448368.12.1e-6189.13PREDICTED: very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 isoform X1 [Cucu... [more]
XP_004148217.12.1e-6189.13very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 [Cucumis sativus] >KGN47357... [more]
XP_022148455.11.4e-5783.33very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 2 [Momordica charantia][more]
BLAST of Lsi10G013460 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G59770.11.3e-2950.43Protein-tyrosine phosphatase-like, PTPLA [more]
AT5G10480.11.1e-0726.55Protein-tyrosine phosphatase-like, PTPLA [more]
AT5G10480.34.1e-0725.27Protein-tyrosine phosphatase-like, PTPLA [more]
AT5G10480.24.5e-0628.57Protein-tyrosine phosphatase-like, PTPLA [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007482Protein-tyrosine phosphatase-like, PTPLAPFAMPF04387PTPLAcoord: 52..138
e-value: 4.2E-18
score: 65.6
IPR007482Protein-tyrosine phosphatase-like, PTPLAPANTHERPTHR11035PTPLA DOMAIN PROTEINcoord: 4..139
NoneNo IPR availablePANTHERPTHR11035:SF35VERY-LONG-CHAIN (3R)-3-HYDROXYACYL-COA DEHYDRATASEcoord: 4..139

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G013460.1Lsi10G013460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030497 fatty acid elongation
biological_process GO:0030148 sphingolipid biosynthetic process
biological_process GO:0042761 very long-chain fatty acid biosynthetic process
cellular_component GO:0030176 integral component of endoplasmic reticulum membrane
molecular_function GO:0018812 3-hydroxyacyl-CoA dehydratase activity
molecular_function GO:0102343 3-hydroxy-arachidoyl-CoA dehydratase activity
molecular_function GO:0102344 3-hydroxy-behenoyl-CoA dehydratase activity
molecular_function GO:0102345 3-hydroxy-lignoceroyl-CoA dehydratase activity
molecular_function GO:0102158 very-long-chain 3-hydroxyacyl-CoA dehydratase activity