Lsi10G013440 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi10G013440
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	RING-type E3 ubiquitin transferase
Location	chr10: 17677598 .. 17678209 (-)
RNA-Seq Expression	Lsi10G013440
Synteny	Lsi10G013440

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCATCGAGTCCGCCGCCGCCATCTCCGGTGCCTGATTACTCTAGCTGGAGTCCATGGTCAATCTCGACCATCGCAATCGTATGCATCTTCTTCCTACTGTTGAGCAACTACAGGATATTGAAACGACTGTTCCGTGTCCTTCATGGAGTGATTTCCGGTCGACCTATGGTCGAAAACCCTAATAATCCTTCCCCGCAAATCCATGGCCATGGATTGGAGGCCTCAACGATTCATTTGCTACCTATTTCACAGTTCAAAAAGAATGAAGAATCCGAATCAACGGCAAGCTTCAATACGGAATGCGCTGTTTGTTTGGGTGAATTCGAAGAAGGAGAATTGATTAAACAATTACCAAATTGCAATCACTCGTTTCATAGTTCTTGCATCGACGCCTGGTTCCGTAATCATTCAAGTTGCCCTCTCTGCAGATTGCAAGTCTCGAGTTTCTCTACTCCTGATTCTTCTTCGGCAATGCTGGAGACGCTTGGAAGAGAAAACGTTCTTCGAGAAAGAGTAGCGCATTATCAAACACTTCGTGCTCAAATTCTGCAGCACTCGGAATTGAGAAGAGATGCATCGTTTCAAGAATCAGATCAACGAAACCGATGA

mRNA sequence

ATGCCATCGAGTCCGCCGCCGCCATCTCCGGTGCCTGATTACTCTAGCTGGAGTCCATGGTCAATCTCGACCATCGCAATCGTATGCATCTTCTTCCTACTGTTGAGCAACTACAGGATATTGAAACGACTGTTCCGTGTCCTTCATGGAGTGATTTCCGGTCGACCTATGGTCGAAAACCCTAATAATCCTTCCCCGCAAATCCATGGCCATGGATTGGAGGCCTCAACGATTCATTTGCTACCTATTTCACAGTTCAAAAAGAATGAAGAATCCGAATCAACGGCAAGCTTCAATACGGAATGCGCTGTTTGTTTGGGTGAATTCGAAGAAGGAGAATTGATTAAACAATTACCAAATTGCAATCACTCGTTTCATAGTTCTTGCATCGACGCCTGGTTCCGTAATCATTCAAGTTGCCCTCTCTGCAGATTGCAAGTCTCGAGTTTCTCTACTCCTGATTCTTCTTCGGCAATGCTGGAGACGCTTGGAAGAGAAAACGTTCTTCGAGAAAGAGTAGCGCATTATCAAACACTTCGTGCTCAAATTCTGCAGCACTCGGAATTGAGAAGAGATGCATCGTTTCAAGAATCAGATCAACGAAACCGATGA

Coding sequence (CDS)

ATGCCATCGAGTCCGCCGCCGCCATCTCCGGTGCCTGATTACTCTAGCTGGAGTCCATGGTCAATCTCGACCATCGCAATCGTATGCATCTTCTTCCTACTGTTGAGCAACTACAGGATATTGAAACGACTGTTCCGTGTCCTTCATGGAGTGATTTCCGGTCGACCTATGGTCGAAAACCCTAATAATCCTTCCCCGCAAATCCATGGCCATGGATTGGAGGCCTCAACGATTCATTTGCTACCTATTTCACAGTTCAAAAAGAATGAAGAATCCGAATCAACGGCAAGCTTCAATACGGAATGCGCTGTTTGTTTGGGTGAATTCGAAGAAGGAGAATTGATTAAACAATTACCAAATTGCAATCACTCGTTTCATAGTTCTTGCATCGACGCCTGGTTCCGTAATCATTCAAGTTGCCCTCTCTGCAGATTGCAAGTCTCGAGTTTCTCTACTCCTGATTCTTCTTCGGCAATGCTGGAGACGCTTGGAAGAGAAAACGTTCTTCGAGAAAGAGTAGCGCATTATCAAACACTTCGTGCTCAAATTCTGCAGCACTCGGAATTGAGAAGAGATGCATCGTTTCAAGAATCAGATCAACGAAACCGATGA

Protein sequence

MPSSPPPPSPVPDYSSWSPWSISTIAIVCIFFLLLSNYRILKRLFRVLHGVISGRPMVENPNNPSPQIHGHGLEASTIHLLPISQFKKNEESESTASFNTECAVCLGEFEEGELIKQLPNCNHSFHSSCIDAWFRNHSSCPLCRLQVSSFSTPDSSSAMLETLGRENVLRERVAHYQTLRAQILQHSELRRDASFQESDQRNR

Homology

[Close]

BLAST of Lsi10G013440 vs. ExPASy Swiss-Prot
Match: Q9LSW9 (RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.8e-18
Identity = 67/195 (34.36%), Postives = 99/195 (50.77%), Query Frame = 0


Query: 5   PPPPSPV-----PDYSSWSPWSISTIAIVCIFFLLLSNYRILKRLFRVLHGV-ISGRPMV 64
           PPPP P+        +S+   +++ I I+   FLL+S Y  + +     H + I GR  +
Sbjct: 17  PPPPPPIFHRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRFSL 76

Query: 65  ----ENPNNP----SPQIHGHGLEASTIHLLPISQFKK----------NEESESTASFNT 124
                N  +P    SP++   GL+ S I  +PI +FKK           E  E     + 
Sbjct: 77  SRRRRNDQDPLMVYSPELRSRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEEKRSQ 136

Query: 125 ECAVCLGEFEEGELIKQLPNCNHSFHSSCIDAWFRNHSSCPLCRLQVS---SF-----ST 168
           EC+VCL EF++ E ++ +PNC+H FH  CID W +N+++CPLCR +VS   SF     S 
Sbjct: 137 ECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRVSCDTSFPPDRVSA 196

[Close]

BLAST of Lsi10G013440 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.1e-17
Identity = 56/165 (33.94%), Postives = 83/165 (50.30%), Query Frame = 0


Query: 5   PPPPSPVPD------YSSWSPWSISTIAIVCIFFLLLSNYRILKRLFRVLHGVISGRPMV 64
           PPPPS   D       SS+SP  I+ I I+    +L+S Y ++ +     H   S   + 
Sbjct: 36  PPPPSFFLDDDSSSSSSSFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETLN 95

Query: 65  ENPNNPS-------PQIHGHGLEASTIHLLPISQFKKNEESESTASFNTECAVCLGEFEE 124
            N N             +G GL  S I  + + ++K  +         ++C+VCL EFEE
Sbjct: 96  LNHNGEGFFSSTQRISTNGDGLNESMIKSITVYKYKSGDGFVD----GSDCSVCLSEFEE 155

Query: 125 GELIKQLPNCNHSFHSSCIDAWFRNHSSCPLCRLQVSSFSTPDSS 157
            E ++ LP CNH+FH  CID W ++HS+CPLCR  V+  + P +S
Sbjct: 156 NESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRAFVTGVNNPTAS 196

[Close]

BLAST of Lsi10G013440 vs. ExPASy Swiss-Prot
Match: P93823 (RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.2e-16
Identity = 62/192 (32.29%), Postives = 91/192 (47.40%), Query Frame = 0


Query: 6   PPPSPVPDYSSWSP--------------WSISTIAIVCIFFLLLSNYRILKRLFRVLHGV 65
           PPP P P  +  SP               +I+ I I+   FLL+S Y  + +     H +
Sbjct: 16  PPPPPPPSTTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQI 75

Query: 66  -ISGRPMVENPNNP----SPQIHGHGLEASTIHLLPISQFKKNE---ESESTASFNTECA 125
            I  R    +  NP    SP     GL+ S I  +P+ +FKK +     E  +  + EC+
Sbjct: 76  DIFRRRRRSSDQNPLMIYSPHEVNRGLDESAIRAIPVFKFKKRDVVAGEEDQSKNSQECS 135

Query: 126 VCLGEFEEGELIKQLPNCNHSFHSSCIDAWFRNHSSCPLCRLQVSSFSTPDSSSAMLETL 176
           VCL EF+E E ++ +PNC H FH  CID W + +++CPLCR  VS       +S  L+ +
Sbjct: 136 VCLNEFQEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVSC-----EASFTLDLI 195

[Close]

BLAST of Lsi10G013440 vs. ExPASy Swiss-Prot
Match: Q8L9T5 (RING-H2 finger protein ATL2 OS=Arabidopsis thaliana OX=3702 GN=ATL2 PE=2 SV=2)

HSP 1 Score: 88.2 bits (217), Expect = 1.2e-16
Identity = 54/143 (37.76%), Postives = 75/143 (52.45%), Query Frame = 0


Query: 25  IAIVCIFFLLLSNYRILKRLFRVLHGVISGR--PMVENPNNPSPQ----IHGHGLEASTI 84
           + I+ +F  L + + +L+   R L      R   MV    +PS      +   GL+ + I
Sbjct: 41  VVILMVFLHLYARWYLLRARRRHLRRRSRNRRATMVFFTADPSTAATSVVASRGLDPNVI 100

Query: 85  HLLPISQFKKNEESESTASFNTECAVCLGEFEEGELIKQLPNCNHSFHSSCIDAWFRNHS 144
             LP+  F     S+ T     ECAVCL EFEE E  + LPNC H+FH  CID WF +HS
Sbjct: 101 KSLPVFTF-----SDETHKDPIECAVCLSEFEESETGRVLPNCQHTFHVDCIDMWFHSHS 160

Query: 145 SCPLCRLQVSSFSTPDSSSAMLE 162
           +CPLCR  V S +  +S++A  E
Sbjct: 161 TCPLCRSLVESLAGIESTAAARE 178

[Close]

BLAST of Lsi10G013440 vs. ExPASy Swiss-Prot
Match: Q7XLY8 (E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0590900 PE=2 SV=2)

HSP 1 Score: 83.2 bits (204), Expect = 3.8e-15
Identity = 52/173 (30.06%), Postives = 85/173 (49.13%), Query Frame = 0


Query: 5   PPPPSPVPDYSS-------WSPWSISTIAIVCIFFLLLSNYRILKRLFRVLHGVISGRPM 64
           PPPP  V   S+       +SP  I+ I ++   FLL+S Y  + +    +   + GR  
Sbjct: 36  PPPPFDVAGTSADDSSGPVFSPLVIAIIGVLASAFLLVSYYTFISKYCGTVSS-LRGRVF 95

Query: 65  VENPNNP-------SPQIHGH---------------GLEASTIHLLPISQFKKNEESEST 124
             +           S   HGH               GL+ + I+ + + ++++ +    T
Sbjct: 96  GSSSGGAAYGGGAGSGGRHGHGQSRSHESWNVSPPSGLDETLINKITVCKYRRGDGFVHT 155

Query: 125 ASFNTECAVCLGEFEEGELIKQLPNCNHSFHSSCIDAWFRNHSSCPLCRLQVS 149
               T+C+VCLGEF +GE ++ LP C+H+FH  CID W ++HS+CPLCR  ++
Sbjct: 156 ----TDCSVCLGEFSDGESLRLLPRCSHAFHQQCIDTWLKSHSNCPLCRANIT 203

[Close]

BLAST of Lsi10G013440 vs. ExPASy TrEMBL
Match: A0A0A0KCA5 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G303210 PE=3 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 4.2e-94
Identity = 180/207 (86.96%), Postives = 186/207 (89.86%), Query Frame = 0


Query: 1   MPSSPPPPSPVPDYSSWSPWSISTIAIVCIFFLLLSNYRILKRLFRVLHGVISGRPMV-- 60
           MPSSPPPPSPVPDYSSWSPWSISTI +VCI FLLLSNYR+LK+L RVLHG+ +GRP V  
Sbjct: 1   MPSSPPPPSPVPDYSSWSPWSISTIVVVCIVFLLLSNYRLLKQLCRVLHGLFTGRPTVQF 60

Query: 61  -ENPNNPSPQIHGHGLEASTIHLLPISQFKKNEESESTASFNTECAVCLGEFEEGELIKQ 120
            ENPNNPS QIHGHGLE S I LLPISQFKKNEESEST SFNTECAVCLGEFEEGELIK 
Sbjct: 61  EENPNNPSLQIHGHGLETSMIRLLPISQFKKNEESESTTSFNTECAVCLGEFEEGELIKH 120

Query: 121 LPNCNHSFHSSCIDAWFRNHSSCPLCRLQVSSFSTPD-SSSAMLETLGRENVLRERVAHY 180
           LPNCNHSFHS CIDAWFRNHSSCPLCRLQV SFSTPD SSSAMLETLGRENVLRERVAHY
Sbjct: 121 LPNCNHSFHSPCIDAWFRNHSSCPLCRLQVLSFSTPDSSSSAMLETLGRENVLRERVAHY 180

Query: 181 QTLRAQILQHSELRRDASFQESDQRNR 204
           QTLRAQILQH E RRDAS Q +DQRNR
Sbjct: 181 QTLRAQILQHPEFRRDASIQGTDQRNR 207

[Close]

BLAST of Lsi10G013440 vs. ExPASy TrEMBL
Match: A0A5A7V3S2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold250G00390 PE=3 SV=1)

HSP 1 Score: 347.4 bits (890), Expect = 3.9e-92
Identity = 175/205 (85.37%), Postives = 182/205 (88.78%), Query Frame = 0


Query: 1   MPSSPPPPSPVPDYSSWSPWSISTIAIVCIFFLLLSNYRILKRLFRVLHGVISGRPMV-- 60
           MPSSPPPPSPVPDYSSWSPWSISTI IVC+ FLLLSNYR+LK+L RVLH + SGRP V  
Sbjct: 1   MPSSPPPPSPVPDYSSWSPWSISTIVIVCVIFLLLSNYRLLKQLCRVLHELFSGRPAVQF 60

Query: 61  -ENPNNPSPQIHGHGLEASTIHLLPISQFKKNEESESTASFNTECAVCLGEFEEGELIKQ 120
            ENPNNPS QIH HGLE S I LLPISQFKK EESEST SFNTECAVCLGEFEEGELIK 
Sbjct: 61  EENPNNPSLQIHAHGLETSMIRLLPISQFKKKEESESTTSFNTECAVCLGEFEEGELIKN 120

Query: 121 LPNCNHSFHSSCIDAWFRNHSSCPLCRLQVSSFSTPDSSSAMLETLGRENVLRERVAHYQ 180
           LPNCNHSFHSSCIDAWFR+HSSCPLCRLQV SFSTPDSSSAMLETLGREN+LRERVAHYQ
Sbjct: 121 LPNCNHSFHSSCIDAWFRDHSSCPLCRLQVWSFSTPDSSSAMLETLGRENILRERVAHYQ 180

Query: 181 TLRAQILQHSELRRDASFQESDQRN 203
           TLRAQILQH E RRD S Q SDQR+
Sbjct: 181 TLRAQILQHPEFRRDESIQGSDQRD 205

[Close]

BLAST of Lsi10G013440 vs. ExPASy TrEMBL
Match: A0A1S3BIX6 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103490579 PE=3 SV=1)

HSP 1 Score: 347.4 bits (890), Expect = 3.9e-92
Identity = 175/205 (85.37%), Postives = 182/205 (88.78%), Query Frame = 0


Query: 1   MPSSPPPPSPVPDYSSWSPWSISTIAIVCIFFLLLSNYRILKRLFRVLHGVISGRPMV-- 60
           MPSSPPPPSPVPDYSSWSPWSISTI IVC+ FLLLSNYR+LK+L RVLH + SGRP V  
Sbjct: 1   MPSSPPPPSPVPDYSSWSPWSISTIVIVCVIFLLLSNYRLLKQLCRVLHELFSGRPAVQF 60

Query: 61  -ENPNNPSPQIHGHGLEASTIHLLPISQFKKNEESESTASFNTECAVCLGEFEEGELIKQ 120
            ENPNNPS QIH HGLE S I LLPISQFKK EESEST SFNTECAVCLGEFEEGELIK 
Sbjct: 61  EENPNNPSLQIHAHGLETSMIRLLPISQFKKKEESESTTSFNTECAVCLGEFEEGELIKN 120

Query: 121 LPNCNHSFHSSCIDAWFRNHSSCPLCRLQVSSFSTPDSSSAMLETLGRENVLRERVAHYQ 180
           LPNCNHSFHSSCIDAWFR+HSSCPLCRLQV SFSTPDSSSAMLETLGREN+LRERVAHYQ
Sbjct: 121 LPNCNHSFHSSCIDAWFRDHSSCPLCRLQVWSFSTPDSSSAMLETLGRENILRERVAHYQ 180

Query: 181 TLRAQILQHSELRRDASFQESDQRN 203
           TLRAQILQH E RRD S Q SDQR+
Sbjct: 181 TLRAQILQHPEFRRDESIQGSDQRD 205

[Close]

BLAST of Lsi10G013440 vs. ExPASy TrEMBL
Match: A0A2I4HVS2 (RING-type E3 ubiquitin transferase OS=Juglans regia OX=51240 GN=LOC109021964 PE=3 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 1.6e-37
Identity = 92/194 (47.42%), Postives = 120/194 (61.86%), Query Frame = 0


Query: 6   PPPSPVPDYSSWSPWSISTIAIVCIFFLLLSNYRILKRLFRVLHGVIS----GRPMVE-- 65
           P P+  P   + + W I  IA VC   +L S YRILK L+   +GVIS    GR ++   
Sbjct: 14  PAPASEPGIPTKNAWEIPLIATVCSVIILFSYYRILKPLYCTNYGVISRNRVGRQILNEA 73

Query: 66  NPNNPSPQIHGHGLEASTIHLLPISQFKKNEESESTASFNTECAVCLGEFEEGELIKQLP 125
           NP++PS Q H H LE+S ++ LPISQF+K++E E T    T+CAVCLGEFEEG+ +K+LP
Sbjct: 74  NPDDPSLQFHSHALESSILNSLPISQFEKSKEGEET-QITTDCAVCLGEFEEGQWLKRLP 133

Query: 126 NCNHSFHSSCIDAWFRNHSSCPLCRLQV------SSFSTPDSSSAMLETLGRENVLRERV 185
            C H+FH SCID WF+ HS+CPLCR  V        F +P S   + + L RE+   ER 
Sbjct: 134 ICTHAFHVSCIDTWFQTHSNCPLCRSHVHDLALHHEFESPVSQHTVPQILRREDFFHERA 193

Query: 186 AHYQTLRAQILQHS 188
            HYQ LR+ IL  S
Sbjct: 194 EHYQILRSTILTTS 206

[Close]

BLAST of Lsi10G013440 vs. ExPASy TrEMBL
Match: W9SMD5 (RING-type E3 ubiquitin transferase OS=Morus notabilis OX=981085 GN=L484_010848 PE=3 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 6.2e-37
Identity = 87/190 (45.79%), Postives = 123/190 (64.74%), Query Frame = 0


Query: 16  SWSPWSISTIAIVCIFFLLLSNYRIL-------KRLFRVLHGVISGRPMVE-NPNNPSPQ 75
           S +PW I+++ ++C   +L S  RI+       +R+    +GV   + + E N ++PS +
Sbjct: 2   SSNPWLIASVVMICAIIILFSYLRIVVLFCWPPQRINFSRNGVPRRQLLDESNTDDPSLR 61

Query: 76  IHGHGLEASTIHLLPISQFKKNEESESTASFNTECAVCLGEFEEGELIKQLPNCNHSFHS 135
              HGLE ST+H LPI+QFK+  E E   + +T+CAVCLGEF+EGE ++ LP+C+HSFH 
Sbjct: 62  FDSHGLEFSTMHSLPITQFKEESEGEMNKTRSTDCAVCLGEFKEGEWLRHLPSCSHSFHI 121

Query: 136 SCIDAWFRNHSSCPLCRLQVSSFSTPDSSSAML-----ETLGRENVLRERVAHYQTLRAQ 193
           SCID WF NH++CPLCR +V S    D  S +      +TL RE+  RER AHY+ LR+Q
Sbjct: 122 SCIDTWFLNHANCPLCRTEVQSLKLEDLESPVSHHSPPQTLRREDFFRERAAHYELLRSQ 181

[Close]

BLAST of Lsi10G013440 vs. NCBI nr
Match: XP_031743630.1 (RING-H2 finger protein ATL52-like [Cucumis sativus] >KGN47355.1 hypothetical protein Csa_023057 [Cucumis sativus])

HSP 1 Score: 354.0 bits (907), Expect = 8.7e-94
Identity = 180/207 (86.96%), Postives = 186/207 (89.86%), Query Frame = 0


Query: 1   MPSSPPPPSPVPDYSSWSPWSISTIAIVCIFFLLLSNYRILKRLFRVLHGVISGRPMV-- 60
           MPSSPPPPSPVPDYSSWSPWSISTI +VCI FLLLSNYR+LK+L RVLHG+ +GRP V  
Sbjct: 1   MPSSPPPPSPVPDYSSWSPWSISTIVVVCIVFLLLSNYRLLKQLCRVLHGLFTGRPTVQF 60

Query: 61  -ENPNNPSPQIHGHGLEASTIHLLPISQFKKNEESESTASFNTECAVCLGEFEEGELIKQ 120
            ENPNNPS QIHGHGLE S I LLPISQFKKNEESEST SFNTECAVCLGEFEEGELIK 
Sbjct: 61  EENPNNPSLQIHGHGLETSMIRLLPISQFKKNEESESTTSFNTECAVCLGEFEEGELIKH 120

Query: 121 LPNCNHSFHSSCIDAWFRNHSSCPLCRLQVSSFSTPD-SSSAMLETLGRENVLRERVAHY 180
           LPNCNHSFHS CIDAWFRNHSSCPLCRLQV SFSTPD SSSAMLETLGRENVLRERVAHY
Sbjct: 121 LPNCNHSFHSPCIDAWFRNHSSCPLCRLQVLSFSTPDSSSSAMLETLGRENVLRERVAHY 180

Query: 181 QTLRAQILQHSELRRDASFQESDQRNR 204
           QTLRAQILQH E RRDAS Q +DQRNR
Sbjct: 181 QTLRAQILQHPEFRRDASIQGTDQRNR 207

[Close]

BLAST of Lsi10G013440 vs. NCBI nr
Match: XP_008448367.1 (PREDICTED: RING-H2 finger protein ATL66-like [Cucumis melo] >KAA0061954.1 RING-H2 finger protein ATL66-like [Cucumis melo var. makuwa] >TYK23976.1 RING-H2 finger protein ATL66-like [Cucumis melo var. makuwa])

HSP 1 Score: 347.4 bits (890), Expect = 8.1e-92
Identity = 175/205 (85.37%), Postives = 182/205 (88.78%), Query Frame = 0


Query: 1   MPSSPPPPSPVPDYSSWSPWSISTIAIVCIFFLLLSNYRILKRLFRVLHGVISGRPMV-- 60
           MPSSPPPPSPVPDYSSWSPWSISTI IVC+ FLLLSNYR+LK+L RVLH + SGRP V  
Sbjct: 1   MPSSPPPPSPVPDYSSWSPWSISTIVIVCVIFLLLSNYRLLKQLCRVLHELFSGRPAVQF 60

Query: 61  -ENPNNPSPQIHGHGLEASTIHLLPISQFKKNEESESTASFNTECAVCLGEFEEGELIKQ 120
            ENPNNPS QIH HGLE S I LLPISQFKK EESEST SFNTECAVCLGEFEEGELIK 
Sbjct: 61  EENPNNPSLQIHAHGLETSMIRLLPISQFKKKEESESTTSFNTECAVCLGEFEEGELIKN 120

Query: 121 LPNCNHSFHSSCIDAWFRNHSSCPLCRLQVSSFSTPDSSSAMLETLGRENVLRERVAHYQ 180
           LPNCNHSFHSSCIDAWFR+HSSCPLCRLQV SFSTPDSSSAMLETLGREN+LRERVAHYQ
Sbjct: 121 LPNCNHSFHSSCIDAWFRDHSSCPLCRLQVWSFSTPDSSSAMLETLGRENILRERVAHYQ 180

Query: 181 TLRAQILQHSELRRDASFQESDQRN 203
           TLRAQILQH E RRD S Q SDQR+
Sbjct: 181 TLRAQILQHPEFRRDESIQGSDQRD 205

[Close]

BLAST of Lsi10G013440 vs. NCBI nr
Match: KAG6570731.1 (RING-H2 finger protein ATL16, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010575.1 RING-H2 finger protein ATL16, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 327.0 bits (837), Expect = 1.1e-85
Identity = 173/213 (81.22%), Postives = 184/213 (86.38%), Query Frame = 0


Query: 1   MPSS---PPPPSPVPDYSSWSPWSISTIAIVCIFFLLLSNYRILKRLFRVLHGVISGRPM 60
           MPSS   PPPPSPVPDYSSWSPWSIS IAIVCI  LLLSNYRILKR  RVLHG+  GR +
Sbjct: 1   MPSSPPPPPPPSPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVLHGLFCGRFV 60

Query: 61  V---ENPNNPSPQIHGHGLEASTIHLLPISQFKKNEESESTASFNTECAVCLGEFEEGEL 120
           V   ENPN+PSP++H HGLEASTI LLPISQFKKNEE+ESTAS NTECAVCLGEFEEGEL
Sbjct: 61  VQFDENPNDPSPEVHVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEEGEL 120

Query: 121 IKQLPNCNHSFHSSCIDAWFRNHSSCPLCRLQVSSFS----TPDSSSAMLETLGRENVLR 180
           IK LPNCNHSFHSSCIDAW RNHSSCPLCRL VS+ S    TPDSSSAMLETLGRE+V R
Sbjct: 121 IKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSIDCPTPDSSSAMLETLGREDVER 180

Query: 181 ERVAHYQTLRAQILQHSELRRDASFQESDQRNR 204
           ERV++YQTLRAQILQ+ ELRRDAS Q SDQRNR
Sbjct: 181 ERVSYYQTLRAQILQNPELRRDASVQGSDQRNR 213

[Close]

BLAST of Lsi10G013440 vs. NCBI nr
Match: XP_023512261.1 (RING-H2 finger protein ATL74-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 321.2 bits (822), Expect = 6.2e-84
Identity = 170/213 (79.81%), Postives = 181/213 (84.98%), Query Frame = 0


Query: 1   MPSS---PPPPSPVPDYSSWSPWSISTIAIVCIFFLLLSNYRILKRLFRVLHGVISGRPM 60
           MPSS   PPPPSPVPDYSSWSPWSIS IAIVCI  LLLSNYRILKR  RV HG+  GR +
Sbjct: 1   MPSSPPPPPPPSPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVFHGLFCGRSV 60

Query: 61  V---ENPNNPSPQIHGHGLEASTIHLLPISQFKKNEESESTASFNTECAVCLGEFEEGEL 120
           V   ENPN+PSPQ+H HGLEASTI LLPISQFKKNEE+ESTAS NTECAVCLGEFEEGEL
Sbjct: 61  VQFDENPNDPSPQVHVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEEGEL 120

Query: 121 IKQLPNCNHSFHSSCIDAWFRNHSSCPLCRLQVSSFS----TPDSSSAMLETLGRENVLR 180
           IK LPNCNHSFHSSCIDAW RNHSSCPLCRL VS+ S    TPDSSSAMLETL RE+V  
Sbjct: 121 IKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSIDCPTPDSSSAMLETLSREDVDG 180

Query: 181 ERVAHYQTLRAQILQHSELRRDASFQESDQRNR 204
           ERV++YQTLRAQILQ+ +LRRDAS Q SDQRNR
Sbjct: 181 ERVSYYQTLRAQILQNPDLRRDASVQGSDQRNR 213

[Close]

BLAST of Lsi10G013440 vs. NCBI nr
Match: KAF3453818.1 (hypothetical protein FNV43_RR04259 [Rhamnella rubrinervis])

HSP 1 Score: 169.9 bits (429), Expect = 2.3e-38
Identity = 94/195 (48.21%), Postives = 127/195 (65.13%), Query Frame = 0


Query: 9   SPVPDYSSWSPWSISTIAIVCIFFLLLSNYRILKRL---FRVLHGVISGRPMVENPNNPS 68
           SP+P  S W+PW+I+++A V I  LLLS +RIL+     F   +   S      N ++PS
Sbjct: 305 SPLPVPSHWNPWAIASVATVSIIILLLSYFRILQSFCCNFSSRNQAGSLLLSETNLDDPS 364

Query: 69  PQIHGHGLEASTIHLLPISQFK-KNEESESTASFNTECAVCLGEFEEGELIKQLPNCNHS 128
            Q H HGLE S IH LPI+QFK +N  S+     +T+CAVCLGEF+EGE ++ LPNC H+
Sbjct: 365 LQFHSHGLEVSLIHSLPITQFKEENGGSKMGHQTSTDCAVCLGEFQEGEWLRFLPNCKHA 424

Query: 129 FHSSCIDAWFRNHSSCPLCRLQVSSFSTPD-----SSSAMLETLGRENVLRERVAHYQTL 188
           FH SCID WF +HS+CPLCR +V+ F+  D     S   +LETL RE+  +ER AHYQ L
Sbjct: 425 FHISCIDTWFLSHSTCPLCRSEVNGFAFEDHEYPVSMYTLLETLRREDFFQERAAHYQFL 484

Query: 189 RAQILQHSELRRDAS 195
           ++ IL +S  R ++S
Sbjct: 485 QSTILNNSVARTESS 499

[Close]

BLAST of Lsi10G013440 vs. TAIR 10
Match: AT5G43420.1 (RING/U-box superfamily protein )

HSP 1 Score: 93.6 bits (231), Expect = 2.0e-19
Identity = 67/195 (34.36%), Postives = 99/195 (50.77%), Query Frame = 0


Query: 5   PPPPSPV-----PDYSSWSPWSISTIAIVCIFFLLLSNYRILKRLFRVLHGV-ISGRPMV 64
           PPPP P+        +S+   +++ I I+   FLL+S Y  + +     H + I GR  +
Sbjct: 17  PPPPPPIFHRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRFSL 76

Query: 65  ----ENPNNP----SPQIHGHGLEASTIHLLPISQFKK----------NEESESTASFNT 124
                N  +P    SP++   GL+ S I  +PI +FKK           E  E     + 
Sbjct: 77  SRRRRNDQDPLMVYSPELRSRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEEKRSQ 136

Query: 125 ECAVCLGEFEEGELIKQLPNCNHSFHSSCIDAWFRNHSSCPLCRLQVS---SF-----ST 168
           EC+VCL EF++ E ++ +PNC+H FH  CID W +N+++CPLCR +VS   SF     S 
Sbjct: 137 ECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRVSCDTSFPPDRVSA 196

[Close]

BLAST of Lsi10G013440 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 91.7 bits (226), Expect = 7.5e-19
Identity = 56/165 (33.94%), Postives = 83/165 (50.30%), Query Frame = 0


Query: 5   PPPPSPVPD------YSSWSPWSISTIAIVCIFFLLLSNYRILKRLFRVLHGVISGRPMV 64
           PPPPS   D       SS+SP  I+ I I+    +L+S Y ++ +     H   S   + 
Sbjct: 36  PPPPSFFLDDDSSSSSSSFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETLN 95

Query: 65  ENPNNPS-------PQIHGHGLEASTIHLLPISQFKKNEESESTASFNTECAVCLGEFEE 124
            N N             +G GL  S I  + + ++K  +         ++C+VCL EFEE
Sbjct: 96  LNHNGEGFFSSTQRISTNGDGLNESMIKSITVYKYKSGDGFVD----GSDCSVCLSEFEE 155

Query: 125 GELIKQLPNCNHSFHSSCIDAWFRNHSSCPLCRLQVSSFSTPDSS 157
            E ++ LP CNH+FH  CID W ++HS+CPLCR  V+  + P +S
Sbjct: 156 NESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRAFVTGVNNPTAS 196

[Close]

BLAST of Lsi10G013440 vs. TAIR 10
Match: AT1G04360.1 (RING/U-box superfamily protein )

HSP 1 Score: 88.2 bits (217), Expect = 8.3e-18
Identity = 62/192 (32.29%), Postives = 91/192 (47.40%), Query Frame = 0


Query: 6   PPPSPVPDYSSWSP--------------WSISTIAIVCIFFLLLSNYRILKRLFRVLHGV 65
           PPP P P  +  SP               +I+ I I+   FLL+S Y  + +     H +
Sbjct: 16  PPPPPPPSTTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQI 75

Query: 66  -ISGRPMVENPNNP----SPQIHGHGLEASTIHLLPISQFKKNE---ESESTASFNTECA 125
            I  R    +  NP    SP     GL+ S I  +P+ +FKK +     E  +  + EC+
Sbjct: 76  DIFRRRRRSSDQNPLMIYSPHEVNRGLDESAIRAIPVFKFKKRDVVAGEEDQSKNSQECS 135

Query: 126 VCLGEFEEGELIKQLPNCNHSFHSSCIDAWFRNHSSCPLCRLQVSSFSTPDSSSAMLETL 176
           VCL EF+E E ++ +PNC H FH  CID W + +++CPLCR  VS       +S  L+ +
Sbjct: 136 VCLNEFQEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVSC-----EASFTLDLI 195

[Close]

BLAST of Lsi10G013440 vs. TAIR 10
Match: AT3G16720.1 (TOXICOS EN LEVADURA 2 )

HSP 1 Score: 88.2 bits (217), Expect = 8.3e-18
Identity = 54/143 (37.76%), Postives = 75/143 (52.45%), Query Frame = 0


Query: 25  IAIVCIFFLLLSNYRILKRLFRVLHGVISGR--PMVENPNNPSPQ----IHGHGLEASTI 84
           + I+ +F  L + + +L+   R L      R   MV    +PS      +   GL+ + I
Sbjct: 41  VVILMVFLHLYARWYLLRARRRHLRRRSRNRRATMVFFTADPSTAATSVVASRGLDPNVI 100

Query: 85  HLLPISQFKKNEESESTASFNTECAVCLGEFEEGELIKQLPNCNHSFHSSCIDAWFRNHS 144
             LP+  F     S+ T     ECAVCL EFEE E  + LPNC H+FH  CID WF +HS
Sbjct: 101 KSLPVFTF-----SDETHKDPIECAVCLSEFEESETGRVLPNCQHTFHVDCIDMWFHSHS 160

Query: 145 SCPLCRLQVSSFSTPDSSSAMLE 162
           +CPLCR  V S +  +S++A  E
Sbjct: 161 TCPLCRSLVESLAGIESTAAARE 178

[Close]

BLAST of Lsi10G013440 vs. TAIR 10
Match: AT5G10380.1 (RING/U-box superfamily protein )

HSP 1 Score: 82.4 bits (202), Expect = 4.5e-16
Identity = 60/197 (30.46%), Postives = 88/197 (44.67%), Query Frame = 0


Query: 2   PSSPPPPSPVPDYSSWSPWSISTIAIVCIF-FLLLSNYRILKRLFRVLHGVISGRPMVEN 61
           P  PPPP P  + S   P SI  +  + +F FL+   Y  + +  R+    ++     + 
Sbjct: 26  PPPPPPPPPPRELSLLLPTSICVVGSIILFLFLVFFLYLHITQQRRISAASVTPGDTNQQ 85

Query: 62  PNNPSP------------QIHGHGLEASTIHLLPISQFKKNEESESTASFNTECAVCLGE 121
            +                QI   GL  S I+ + +  FKK E         TEC+VCL E
Sbjct: 86  EDEDETEERDFSDFHHVWQIPTVGLHRSAINSITVVGFKKGEGIID----GTECSVCLNE 145

Query: 122 FEEGELIKQLPNCNHSFHSSCIDAWFRNHSSCPLCRLQVSSFSTP----------DSSSA 176
           FEE E ++ LP C+H+FH +CID W  +H +CPLCR  V   + P            S +
Sbjct: 146 FEEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCRAPVLLITEPPHQETETNHQPDSES 205

The following BLAST results are available for this feature:

BLAST of Lsi10G013440 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSW9	2.8e-18	34.36	RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1	[more]
Q9LF64	1.1e-17	33.94	RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1	[more]
P93823	1.2e-16	32.29	RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1	[more]
Q8L9T5	1.2e-16	37.76	RING-H2 finger protein ATL2 OS=Arabidopsis thaliana OX=3702 GN=ATL2 PE=2 SV=2	[more]
Q7XLY8	3.8e-15	30.06	E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=3994...	[more]

BLAST of Lsi10G013440 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KCA5	4.2e-94	86.96	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G303210 PE...	[more]
A0A5A7V3S2	3.9e-92	85.37	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3BIX6	3.9e-92	85.37	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103490579 PE=3 ...	[more]
A0A2I4HVS2	1.6e-37	47.42	RING-type E3 ubiquitin transferase OS=Juglans regia OX=51240 GN=LOC109021964 PE=...	[more]
W9SMD5	6.2e-37	45.79	RING-type E3 ubiquitin transferase OS=Morus notabilis OX=981085 GN=L484_010848 P...	[more]

BLAST of Lsi10G013440 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031743630.1	8.7e-94	86.96	RING-H2 finger protein ATL52-like [Cucumis sativus] >KGN47355.1 hypothetical pro...	[more]
XP_008448367.1	8.1e-92	85.37	PREDICTED: RING-H2 finger protein ATL66-like [Cucumis melo] >KAA0061954.1 RING-H...	[more]
KAG6570731.1	1.1e-85	81.22	RING-H2 finger protein ATL16, partial [Cucurbita argyrosperma subsp. sororia] >K...	[more]
XP_023512261.1	6.2e-84	79.81	RING-H2 finger protein ATL74-like [Cucurbita pepo subsp. pepo]	[more]
KAF3453818.1	2.3e-38	48.21	hypothetical protein FNV43_RR04259 [Rhamnella rubrinervis]	[more]

BLAST of Lsi10G013440 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G43420.1	2.0e-19	34.36	RING/U-box superfamily protein	[more]
AT5G17600.1	7.5e-19	33.94	RING/U-box superfamily protein	[more]
AT1G04360.1	8.3e-18	32.29	RING/U-box superfamily protein	[more]
AT3G16720.1	8.3e-18	37.76	TOXICOS EN LEVADURA 2	[more]
AT5G10380.1	4.5e-16	30.46	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 102..143 e-value: 2.0E-7 score: 40.7
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 101..144 e-value: 1.3E-13 score: 51.0
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 102..144 score: 12.622055
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 73..152 e-value: 1.4E-21 score: 77.5
IPR044675	E3 ubiquitin-protein ligase RING1-like	PANTHER	PTHR45676	RING-H2 FINGER PROTEIN ATL51-RELATED	coord: 14..157
None	No IPR available	PANTHER	PTHR45676:SF107	RING/U-BOX SUPERFAMILY PROTEIN	coord: 14..157
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 101..144 e-value: 2.08871E-18 score: 73.1049
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 101..149

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G013440.1	Lsi10G013440.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane