Lsi10G013070 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi10G013070
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	NEDD8-specific protease 1
Location	chr10: 17264571 .. 17265281 (-)
RNA-Seq Expression	Lsi10G013070
Synteny	Lsi10G013070

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGAAGTCTGAAGGAGATGAGAAAATCTTAAGCTACAACGATGTTGTACTCCGAAAATCCGACTTGGATATCCTTAGTGGTCCATATTATCTGAATGATCGAATCATTGAATTCTATTTTAGCTATCTAAGCACAACCCATCCTTCCAAGGACATTCTTCTATCTCCACCCTCAATTGCTTTCTGGATAATGAACTGCCCCGACGCGGAGAGTCTCAACGATTTCTTAAAACCTCTCAATCTTTGCGACAAGAAGCTGGTAATCTTCCCTGTCAACGACAATAATGATGTTACTAAAGCCGAAGGTGGAAACCACTGGAGTCTTCTTGCATTTTACAGAGAAGCGAACGTATTTGTTCATCACGATAGCAACAGAGGGATGAATAAGTACGACGCCAAGCGACTCTACAGAGCTGTGGCTAGATTTATGAGTGGTGACTCGAATTCAACGTCGAATCCGAGTTATGTGGAATGCATCGAGTCTCCTCAGCAAGTAAATGGTTATGATTGTGGTGTATATGTTACAGCTATAGCAAGAACCATATGCAGGTGGTATGAGCCAAGGAAAGAAATTGACAGAGATGGCTTGTGGTTTTCAGCAGTGGTGGAGGAGATTAGTCCATCATTTGTTGCTAATATGCGTAGGGAAATATTGGGGCTAATAAGAAGTTTAATGGCTGTGAAGGAATATCGACCGGCTACTAAATAA

mRNA sequence

ATGGGGAAGTCTGAAGGAGATGAGAAAATCTTAAGCTACAACGATGTTGTACTCCGAAAATCCGACTTGGATATCCTTAGTGGTCCATATTATCTGAATGATCGAATCATTGAATTCTATTTTAGCTATCTAAGCACAACCCATCCTTCCAAGGACATTCTTCTATCTCCACCCTCAATTGCTTTCTGGATAATGAACTGCCCCGACGCGGAGAGTCTCAACGATTTCTTAAAACCTCTCAATCTTTGCGACAAGAAGCTGGTAATCTTCCCTGTCAACGACAATAATGATGTTACTAAAGCCGAAGGTGGAAACCACTGGAGTCTTCTTGCATTTTACAGAGAAGCGAACGTATTTGTTCATCACGATAGCAACAGAGGGATGAATAAGTACGACGCCAAGCGACTCTACAGAGCTGTGGCTAGATTTATGAGTGGTGACTCGAATTCAACGTCGAATCCGAGTTATGTGGAATGCATCGAGTCTCCTCAGCAAGTAAATGGTTATGATTGTGGTGTATATGTTACAGCTATAGCAAGAACCATATGCAGGTGGTATGAGCCAAGGAAAGAAATTGACAGAGATGGCTTGTGGTTTTCAGCAGTGGTGGAGGAGATTAGTCCATCATTTGTTGCTAATATGCGTAGGGAAATATTGGGGCTAATAAGAAGTTTAATGGCTGTGAAGGAATATCGACCGGCTACTAAATAA

Coding sequence (CDS)

ATGGGGAAGTCTGAAGGAGATGAGAAAATCTTAAGCTACAACGATGTTGTACTCCGAAAATCCGACTTGGATATCCTTAGTGGTCCATATTATCTGAATGATCGAATCATTGAATTCTATTTTAGCTATCTAAGCACAACCCATCCTTCCAAGGACATTCTTCTATCTCCACCCTCAATTGCTTTCTGGATAATGAACTGCCCCGACGCGGAGAGTCTCAACGATTTCTTAAAACCTCTCAATCTTTGCGACAAGAAGCTGGTAATCTTCCCTGTCAACGACAATAATGATGTTACTAAAGCCGAAGGTGGAAACCACTGGAGTCTTCTTGCATTTTACAGAGAAGCGAACGTATTTGTTCATCACGATAGCAACAGAGGGATGAATAAGTACGACGCCAAGCGACTCTACAGAGCTGTGGCTAGATTTATGAGTGGTGACTCGAATTCAACGTCGAATCCGAGTTATGTGGAATGCATCGAGTCTCCTCAGCAAGTAAATGGTTATGATTGTGGTGTATATGTTACAGCTATAGCAAGAACCATATGCAGGTGGTATGAGCCAAGGAAAGAAATTGACAGAGATGGCTTGTGGTTTTCAGCAGTGGTGGAGGAGATTAGTCCATCATTTGTTGCTAATATGCGTAGGGAAATATTGGGGCTAATAAGAAGTTTAATGGCTGTGAAGGAATATCGACCGGCTACTAAATAA

Protein sequence

MGKSEGDEKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSTTHPSKDILLSPPSIAFWIMNCPDAESLNDFLKPLNLCDKKLVIFPVNDNNDVTKAEGGNHWSLLAFYREANVFVHHDSNRGMNKYDAKRLYRAVARFMSGDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIARTICRWYEPRKEIDRDGLWFSAVVEEISPSFVANMRREILGLIRSLMAVKEYRPATK

Homology

[Close]

BLAST of Lsi10G013070 vs. ExPASy Swiss-Prot
Match: Q9LSS7 (NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 3.2e-74
Identity = 134/227 (59.03%), Postives = 167/227 (73.57%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSTTHPSKDILLSPPSI 60
           MG +  D+KILSY DVVLR+SDLDIL+GP +LNDR+IEFY S+LST H S  I L PPSI
Sbjct: 1   MGNTSDDDKILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSI 60

Query: 61  AFWIMNCPDAESLNDFLKPLNLCDKKLVIFPVNDNNDVTKAEGGNHWSLLAFYREANVFV 120
           AFWI NCPD E L DF+KPLNL DK L+I PVNDN++V  AEGG HWSLL +Y+EAN FV
Sbjct: 61  AFWISNCPDTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFV 120

Query: 121 HHDSNRGMNKYDAKRLYRAVARFMSGDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDS  G+N++ AK+L++AV+ F+     S  + SY EC ++PQQ NGYDCGV++ A AR
Sbjct: 121 HHDSYMGVNRWSAKQLFKAVSPFV-----SNGDASYKECTDTPQQKNGYDCGVFLLATAR 180

Query: 181 TICRWYEPRKEIDRDGLWFSAVVEEISPSFVANMRREILGLIRSLMA 228
            IC W+      +RD LWF A V+E  P  V ++R EIL LI+ LM+
Sbjct: 181 VICEWFSSGGMKNRDELWF-ANVKETVPDLVNHLREEILALIKKLMS 221

[Close]

BLAST of Lsi10G013070 vs. ExPASy Swiss-Prot
Match: Q54XR2 (Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=senp8 PE=3 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 5.5e-26
Identity = 77/242 (31.82%), Postives = 117/242 (48.35%), Query Frame = 0


Query: 7   DEKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYL------STTHPSKDILLSPPSI 66
           D  I+SYND  + +SD++IL    +LND II FY  +L      +       +LL  PS+
Sbjct: 3   DPLIVSYNDSAIYQSDINILKSNQWLNDSIISFYLEWLKDGGEDNKNKIPNQVLLLSPSV 62

Query: 67  AFWIMNCPDAESLND----FLKPLNLCDKKLVIFPVNDNNDVTKAEGGNHWSLLAFYREA 126
            F    C   ES  +    F +PL+L  K+++ FP+ +N D     GG HWSLL F +  
Sbjct: 63  VFC---CSFVESEQEIQLMFEQPLSLKTKEVIFFPLTNNRDPNVIGGGTHWSLLIFIKSL 122

Query: 127 NVFVHHDSNRGMNKYDAKRLYRAVARFMSGDSNSTSNPSYVECIESPQQVNGYDCGVYVT 186
           N F+++DS    N  DA  +       +S     T+   ++   ++PQQ NGYDCG+YV 
Sbjct: 123 NKFIYYDSINSFNSSDAIFIISKFKFLLSSPPPKTNLKEFLINQKTPQQQNGYDCGLYVL 182

Query: 187 AIARTICRWYEPRKE--------IDRDGLWFS-----AVVEEISPSFVANMRREILGLIR 226
           +I   + +      E        I    L  S      + +EI+P+++ + R EIL  I 
Sbjct: 183 SIIEELLKLIIKENENNKGEENKISYKDLLLSEFTNELLFKEITPNYIKDKRVEILNTIL 241

[Close]

BLAST of Lsi10G013070 vs. ExPASy Swiss-Prot
Match: Q96LD8 (Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.7e-25
Identity = 68/222 (30.63%), Postives = 120/222 (54.05%), Query Frame = 0


Query: 7   DEKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSTT--HPSKD-ILLSPPSIAFW 66
           D  +LSY D +LR+SD+ +L  P +LND II F F Y + +  H   D +    P +  +
Sbjct: 2   DPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVSFISPEVTQF 61

Query: 67  IMNCPDAESLNDFLKPLNLCDKKLVIFPVNDNNDVTKAEGGNHWSLLAFYREANVFVHHD 126
           I    +   +  FL+PL+L +K++V   +NDN++  +A GG HWSLL + ++ N F H+D
Sbjct: 62  IKCTSNPAEIAMFLEPLDLPNKRVVFLAINDNSN--QAAGGTHWSLLVYLQDKNSFFHYD 121

Query: 127 SNRGMNKYDAKRLYRAVARFMSGDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIARTIC 186
           S+   N   AK++   +  F+    +     ++VE  ++P Q N YDCG+YV      +C
Sbjct: 122 SHSRSNSVHAKQVAEKLEAFLGRKGDKL---AFVE-EKAPAQQNSYDCGMYVICNTEALC 181

Query: 187 RWYEPRKEIDRDGLWFSAVVEEISPSFVANMRREILGLIRSL 226
           + +  ++          ++++ ++P+++   R E   LI +L
Sbjct: 182 QNFFRQQT--------ESLLQLLTPAYITKKRGEWKDLITTL 209

[Close]

BLAST of Lsi10G013070 vs. ExPASy Swiss-Prot
Match: Q9D2Z4 (Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2)

HSP 1 Score: 114.8 bits (286), Expect = 1.4e-24
Identity = 70/227 (30.84%), Postives = 115/227 (50.66%), Query Frame = 0


Query: 7   DEKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSTT--HPSKD-ILLSPPSIAFW 66
           D  +LSY D +LR+SD+ +L  P +LND II F F Y + +  H   D +    P +  +
Sbjct: 2   DPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVCFISPEVTQF 61

Query: 67  IMNCPDAESLNDFLKPLNLCDKKLVIFPVNDNNDVTKAEGGNHWSLLAFYREANVFVHHD 126
           I        +  FL+PL+L  K++V   +NDN++  +A GG HWSLL + ++ N F H+D
Sbjct: 62  IKCTSSPAEIAMFLEPLDLPHKRVVFLAINDNSN--QAAGGTHWSLLVYLQDKNSFFHYD 121

Query: 127 SNRGMNKYDAKRLYRAVARFMSGDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIARTIC 186
           S+   N   AK++   +  F+    +      +VE  ++P Q N YDCG+YV      +C
Sbjct: 122 SHSRSNSIHAKQVAEKLKAFLGSKGDKL---VFVE-EKAPAQENSYDCGMYVICNTEALC 181

Query: 187 RWYEPRKEIDRDGLWFSAVVEEISPSFVANMRREILGLIRSLMAVKE 231
           +    R+          + ++ ++P+++   R E   LI  L    E
Sbjct: 182 QSLFRRQP--------ESPLQLLTPTYITKKRGEWKDLIARLAKKNE 214

[Close]

BLAST of Lsi10G013070 vs. ExPASy Swiss-Prot
Match: Q5FVJ8 (Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.8e-24
Identity = 69/222 (31.08%), Postives = 115/222 (51.80%), Query Frame = 0


Query: 7   DEKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSTT--HPSKD-ILLSPPSIAFW 66
           D  +LSY D +LR+SD+ +L  P +LND II F F Y +++  H   D +    P +  +
Sbjct: 2   DPVVLSYMDSLLRQSDVSLLDPPNWLNDHIIGFAFEYFASSQFHDCSDHVCFISPEVTQF 61

Query: 67  IMNCPDAESLNDFLKPLNLCDKKLVIFPVNDNNDVTKAEGGNHWSLLAFYREANVFVHHD 126
           I        +  FL+PL+L  K++V   +NDN++  +A GG HWSLL + ++ N F H+D
Sbjct: 62  IKCTSSPAEIAMFLEPLDLPHKRVVFLAINDNSN--QAAGGTHWSLLVYLQDKNSFFHYD 121

Query: 127 SNRGMNKYDAKRLYRAVARFMSGDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIARTIC 186
           S+   N   AK++   +  F+    +      +VE  ++P Q N YDCG+YV      +C
Sbjct: 122 SHSRSNSIHAKQVAEKLKAFLGSKGDKL---VFVE-EKAPAQQNSYDCGMYVICNTEALC 181

Query: 187 RWYEPRKEIDRDGLWFSAVVEEISPSFVANMRREILGLIRSL 226
           +    R+          + ++ ++P+++   R E   LI  L
Sbjct: 182 QNLFRRQP--------ESPLQLLTPTYITKKRGEWKDLIARL 209

[Close]

BLAST of Lsi10G013070 vs. ExPASy TrEMBL
Match: A0A5D3CSG4 (NEDD8-specific protease 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00810 PE=3 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 1.7e-118
Identity = 204/236 (86.44%), Postives = 223/236 (94.49%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSTTHPSKDILLSPPSI 60
           MGK EGDEKILS+NDVVLRKSDL+ILSGPY+LNDRIIEFYFSYLSTTHPS DILLSPPSI
Sbjct: 1   MGKFEGDEKILSFNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDILLSPPSI 60

Query: 61  AFWIMNCPDAESLNDFLKPLNLCDKKLVIFPVNDNNDVTKAEGGNHWSLLAFYREANVFV 120
           AFWIMNCPD ESLN FL+PLNL  KKLVIFPVNDN+DV+K EGGNHWSLLAFYREAN+FV
Sbjct: 61  AFWIMNCPDVESLNGFLEPLNLRHKKLVIFPVNDNSDVSKPEGGNHWSLLAFYREANIFV 120

Query: 121 HHDSNRGMNKYDAKRLYRAVARFMSGDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDSN+GMNKYDAKRLY+AVARFM+  SNSTSNPSY+EC+ESPQQVNGYDCGVYVTAIAR
Sbjct: 121 HHDSNKGMNKYDAKRLYKAVARFMNDGSNSTSNPSYMECVESPQQVNGYDCGVYVTAIAR 180

Query: 181 TICRWYEPRKEIDRDGLWFSAVVEEISPSFVANMRREILGLIRSLMAVKEYRPATK 237
           +IC+WYE R+E+++DGLWFSAVVEEISP  VANMRREIL LIRSLMAVKEY+PATK
Sbjct: 181 SICKWYEERREVEKDGLWFSAVVEEISPLLVANMRREILELIRSLMAVKEYQPATK 236

[Close]

BLAST of Lsi10G013070 vs. ExPASy TrEMBL
Match: A0A1S3CK81 (NEDD8-specific protease 1 OS=Cucumis melo OX=3656 GN=LOC103501907 PE=3 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 1.7e-118
Identity = 204/236 (86.44%), Postives = 223/236 (94.49%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSTTHPSKDILLSPPSI 60
           MGK EGDEKILS+NDVVLRKSDL+ILSGPY+LNDRIIEFYFSYLSTTHPS DILLSPPSI
Sbjct: 1   MGKFEGDEKILSFNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDILLSPPSI 60

Query: 61  AFWIMNCPDAESLNDFLKPLNLCDKKLVIFPVNDNNDVTKAEGGNHWSLLAFYREANVFV 120
           AFWIMNCPD ESLN FL+PLNL  KKLVIFPVNDN+DV+K EGGNHWSLLAFYREAN+FV
Sbjct: 61  AFWIMNCPDVESLNGFLEPLNLRHKKLVIFPVNDNSDVSKPEGGNHWSLLAFYREANIFV 120

Query: 121 HHDSNRGMNKYDAKRLYRAVARFMSGDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDSN+GMNKYDAKRLY+AVARFM+  SNSTSNPSY+EC+ESPQQVNGYDCGVYVTAIAR
Sbjct: 121 HHDSNKGMNKYDAKRLYKAVARFMNDGSNSTSNPSYMECVESPQQVNGYDCGVYVTAIAR 180

Query: 181 TICRWYEPRKEIDRDGLWFSAVVEEISPSFVANMRREILGLIRSLMAVKEYRPATK 237
           +IC+WYE R+E+++DGLWFSAVVEEISP  VANMRREIL LIRSLMAVKEY+PATK
Sbjct: 181 SICKWYEERREVEKDGLWFSAVVEEISPLLVANMRREILELIRSLMAVKEYQPATK 236

[Close]

BLAST of Lsi10G013070 vs. ExPASy TrEMBL
Match: A0A0A0KEM9 (ULP_PROTEASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G289720 PE=3 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 3.2e-117
Identity = 204/236 (86.44%), Postives = 220/236 (93.22%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSTTHPSKDILLSPPSI 60
           MGKSEGDEKILSYNDVVLRKSDL+ILSGPY+LNDRIIEFYFSYLSTTHPS  ILLSPPSI
Sbjct: 1   MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNGILLSPPSI 60

Query: 61  AFWIMNCPDAESLNDFLKPLNLCDKKLVIFPVNDNNDVTKAEGGNHWSLLAFYREANVFV 120
           AFW+MNCPD ESLN FL+PLNL  KKLVIFPVNDN DV+K EGGNHWSLLAFYREAN+FV
Sbjct: 61  AFWMMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFV 120

Query: 121 HHDSNRGMNKYDAKRLYRAVARFMSGDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDSN+GMNKY AKRLY AVARFM+  S ST NPSYVEC+ESPQQVNGYDCGVYVTAIAR
Sbjct: 121 HHDSNKGMNKYAAKRLYNAVARFMNDGSISTLNPSYVECVESPQQVNGYDCGVYVTAIAR 180

Query: 181 TICRWYEPRKEIDRDGLWFSAVVEEISPSFVANMRREILGLIRSLMAVKEYRPATK 237
           +IC+WYE R+E+++DGLWFSAVVEEISPS VANMRREILGLIRSLMAVKEY+PATK
Sbjct: 181 SICKWYEERREVEKDGLWFSAVVEEISPSLVANMRREILGLIRSLMAVKEYQPATK 236

[Close]

BLAST of Lsi10G013070 vs. ExPASy TrEMBL
Match: A0A6J1CWB2 (NEDD8-specific protease 1 OS=Momordica charantia OX=3673 GN=LOC111015386 PE=3 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 3.7e-110
Identity = 191/236 (80.93%), Postives = 214/236 (90.68%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSTTHPSKDILLSPPSI 60
           M KSEGD+KILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLS+ HPSKDILL+PPSI
Sbjct: 1   MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI 60

Query: 61  AFWIMNCPDAESLNDFLKPLNLCDKKLVIFPVNDNNDVTKAEGGNHWSLLAFYREANVFV 120
           AFW MNCPD+ SL+DFL+PL+L +KKLVIFPVNDN+DV+KAEGGNHWSLLAFYRE+N F+
Sbjct: 61  AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI 120

Query: 121 HHDSNRGMNKYDAKRLYRAVARFMSGDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDSNRGMNK+ AKRLY+AVARF S DSN TS P YVEC+ESPQQVNGYDCG+YVTAIAR
Sbjct: 121 HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR 180

Query: 181 TICRWYEPRKEIDRDGLWFSAVVEEISPSFVANMRREILGLIRSLMAVKEYRPATK 237
           +IC WY  RKE+D DG WF AVVEE++P  VANMR EIL LIRSLMAVKEY+PA++
Sbjct: 181 SICSWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMAVKEYQPASR 236

[Close]

BLAST of Lsi10G013070 vs. ExPASy TrEMBL
Match: A0A6J1KYI9 (NEDD8-specific protease 1 OS=Cucurbita maxima OX=3661 GN=LOC111499890 PE=3 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 1.2e-103
Identity = 191/239 (79.92%), Postives = 212/239 (88.70%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSTTHPSKDILLSPPSI 60
           MGKSEGDEKILSYNDVVLRKSDLDIL+GP YLNDRIIEFYFSYL T HPS+DILLSPPSI
Sbjct: 1   MGKSEGDEKILSYNDVVLRKSDLDILNGPCYLNDRIIEFYFSYLCTCHPSEDILLSPPSI 60

Query: 61  AFWIMNCPDAESLNDFLKPLNLCDKKLVIFPVNDNNDVTKAEGGNHWSLLAFYREANVFV 120
           AFWIMNCPD + LNDFL+PLNL  + LVIFPVNDNNDV+KAEGGNHWS+L FYREANVFV
Sbjct: 61  AFWIMNCPDTDGLNDFLQPLNLRVRNLVIFPVNDNNDVSKAEGGNHWSVLVFYREANVFV 120

Query: 121 HHDSNRGMNKYDAKRLYRAVARFMSGDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDS +G+N   AKRLYRAVARFM G +NSTS PSYVEC ESPQQVNG+DCG+YVTAI+R
Sbjct: 121 HHDSCKGVNNSSAKRLYRAVARFM-GVTNSTSEPSYVECSESPQQVNGHDCGLYVTAISR 180

Query: 181 TICRWYE-PRKEI-DRDG-LWFSAVVEEISPSFVANMRREILGLIRSLMAVKEYRPATK 237
           +ICRWYE  RKE    DG LWFSAVV+E++PS VA+MR EILGLIRS MA+KEY+PA+K
Sbjct: 181 SICRWYEQERKEAGGGDGFLWFSAVVKEVTPSSVADMRSEILGLIRSFMALKEYQPASK 238

[Close]

BLAST of Lsi10G013070 vs. NCBI nr
Match: XP_038902669.1 (NEDD8-specific protease 1 [Benincasa hispida])

HSP 1 Score: 460.7 bits (1184), Expect = 7.7e-126
Identity = 217/236 (91.95%), Postives = 228/236 (96.61%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSTTHPSKDILLSPPSI 60
           MGKSEGDEKILSYNDVVLRKSDLDIL GPYYLNDRI EFYFSYLSTTHPSKDILLSPPSI
Sbjct: 1   MGKSEGDEKILSYNDVVLRKSDLDILGGPYYLNDRIPEFYFSYLSTTHPSKDILLSPPSI 60

Query: 61  AFWIMNCPDAESLNDFLKPLNLCDKKLVIFPVNDNNDVTKAEGGNHWSLLAFYREANVFV 120
           AFWIMNCPDAESL+DFLKPLNLCDKKLVIFPVNDNNDV+KAEGGNHWSLLAFYREANVFV
Sbjct: 61  AFWIMNCPDAESLDDFLKPLNLCDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYREANVFV 120

Query: 121 HHDSNRGMNKYDAKRLYRAVARFMSGDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDS++GMNKY AKRLYRAVARFMS DSNSTSNPSY+EC+ESPQQVNGYDCGVYV AIAR
Sbjct: 121 HHDSHKGMNKYHAKRLYRAVARFMSADSNSTSNPSYLECVESPQQVNGYDCGVYVIAIAR 180

Query: 181 TICRWYEPRKEIDRDGLWFSAVVEEISPSFVANMRREILGLIRSLMAVKEYRPATK 237
           TICRWYE RKE+DRDGLWFSAVV+E+SPSFV NMR EILGLIRSLMAVKEY+PA+K
Sbjct: 181 TICRWYEQRKEVDRDGLWFSAVVKEVSPSFVTNMRSEILGLIRSLMAVKEYQPASK 236

[Close]

BLAST of Lsi10G013070 vs. NCBI nr
Match: XP_008463889.1 (PREDICTED: NEDD8-specific protease 1 [Cucumis melo] >KAA0035294.1 NEDD8-specific protease 1 [Cucumis melo var. makuwa] >TYK14342.1 NEDD8-specific protease 1 [Cucumis melo var. makuwa])

HSP 1 Score: 435.3 bits (1118), Expect = 3.5e-118
Identity = 204/236 (86.44%), Postives = 223/236 (94.49%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSTTHPSKDILLSPPSI 60
           MGK EGDEKILS+NDVVLRKSDL+ILSGPY+LNDRIIEFYFSYLSTTHPS DILLSPPSI
Sbjct: 1   MGKFEGDEKILSFNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDILLSPPSI 60

Query: 61  AFWIMNCPDAESLNDFLKPLNLCDKKLVIFPVNDNNDVTKAEGGNHWSLLAFYREANVFV 120
           AFWIMNCPD ESLN FL+PLNL  KKLVIFPVNDN+DV+K EGGNHWSLLAFYREAN+FV
Sbjct: 61  AFWIMNCPDVESLNGFLEPLNLRHKKLVIFPVNDNSDVSKPEGGNHWSLLAFYREANIFV 120

Query: 121 HHDSNRGMNKYDAKRLYRAVARFMSGDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDSN+GMNKYDAKRLY+AVARFM+  SNSTSNPSY+EC+ESPQQVNGYDCGVYVTAIAR
Sbjct: 121 HHDSNKGMNKYDAKRLYKAVARFMNDGSNSTSNPSYMECVESPQQVNGYDCGVYVTAIAR 180

Query: 181 TICRWYEPRKEIDRDGLWFSAVVEEISPSFVANMRREILGLIRSLMAVKEYRPATK 237
           +IC+WYE R+E+++DGLWFSAVVEEISP  VANMRREIL LIRSLMAVKEY+PATK
Sbjct: 181 SICKWYEERREVEKDGLWFSAVVEEISPLLVANMRREILELIRSLMAVKEYQPATK 236

[Close]

BLAST of Lsi10G013070 vs. NCBI nr
Match: XP_004149559.1 (NEDD8-specific protease 1 [Cucumis sativus] >XP_031742683.1 NEDD8-specific protease 1 [Cucumis sativus] >XP_031742684.1 NEDD8-specific protease 1 [Cucumis sativus] >KGN47289.1 hypothetical protein Csa_022955 [Cucumis sativus])

HSP 1 Score: 431.0 bits (1107), Expect = 6.5e-117
Identity = 204/236 (86.44%), Postives = 220/236 (93.22%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSTTHPSKDILLSPPSI 60
           MGKSEGDEKILSYNDVVLRKSDL+ILSGPY+LNDRIIEFYFSYLSTTHPS  ILLSPPSI
Sbjct: 1   MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNGILLSPPSI 60

Query: 61  AFWIMNCPDAESLNDFLKPLNLCDKKLVIFPVNDNNDVTKAEGGNHWSLLAFYREANVFV 120
           AFW+MNCPD ESLN FL+PLNL  KKLVIFPVNDN DV+K EGGNHWSLLAFYREAN+FV
Sbjct: 61  AFWMMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFV 120

Query: 121 HHDSNRGMNKYDAKRLYRAVARFMSGDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDSN+GMNKY AKRLY AVARFM+  S ST NPSYVEC+ESPQQVNGYDCGVYVTAIAR
Sbjct: 121 HHDSNKGMNKYAAKRLYNAVARFMNDGSISTLNPSYVECVESPQQVNGYDCGVYVTAIAR 180

Query: 181 TICRWYEPRKEIDRDGLWFSAVVEEISPSFVANMRREILGLIRSLMAVKEYRPATK 237
           +IC+WYE R+E+++DGLWFSAVVEEISPS VANMRREILGLIRSLMAVKEY+PATK
Sbjct: 181 SICKWYEERREVEKDGLWFSAVVEEISPSLVANMRREILGLIRSLMAVKEYQPATK 236

[Close]

BLAST of Lsi10G013070 vs. NCBI nr
Match: XP_022146090.1 (NEDD8-specific protease 1 [Momordica charantia])

HSP 1 Score: 407.5 bits (1046), Expect = 7.7e-110
Identity = 191/236 (80.93%), Postives = 214/236 (90.68%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSTTHPSKDILLSPPSI 60
           M KSEGD+KILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLS+ HPSKDILL+PPSI
Sbjct: 1   MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI 60

Query: 61  AFWIMNCPDAESLNDFLKPLNLCDKKLVIFPVNDNNDVTKAEGGNHWSLLAFYREANVFV 120
           AFW MNCPD+ SL+DFL+PL+L +KKLVIFPVNDN+DV+KAEGGNHWSLLAFYRE+N F+
Sbjct: 61  AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI 120

Query: 121 HHDSNRGMNKYDAKRLYRAVARFMSGDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDSNRGMNK+ AKRLY+AVARF S DSN TS P YVEC+ESPQQVNGYDCG+YVTAIAR
Sbjct: 121 HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR 180

Query: 181 TICRWYEPRKEIDRDGLWFSAVVEEISPSFVANMRREILGLIRSLMAVKEYRPATK 237
           +IC WY  RKE+D DG WF AVVEE++P  VANMR EIL LIRSLMAVKEY+PA++
Sbjct: 181 SICSWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMAVKEYQPASR 236

[Close]

BLAST of Lsi10G013070 vs. NCBI nr
Match: XP_023007377.1 (NEDD8-specific protease 1 [Cucurbita maxima])

HSP 1 Score: 386.0 bits (990), Expect = 2.4e-103
Identity = 191/239 (79.92%), Postives = 212/239 (88.70%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSTTHPSKDILLSPPSI 60
           MGKSEGDEKILSYNDVVLRKSDLDIL+GP YLNDRIIEFYFSYL T HPS+DILLSPPSI
Sbjct: 1   MGKSEGDEKILSYNDVVLRKSDLDILNGPCYLNDRIIEFYFSYLCTCHPSEDILLSPPSI 60

Query: 61  AFWIMNCPDAESLNDFLKPLNLCDKKLVIFPVNDNNDVTKAEGGNHWSLLAFYREANVFV 120
           AFWIMNCPD + LNDFL+PLNL  + LVIFPVNDNNDV+KAEGGNHWS+L FYREANVFV
Sbjct: 61  AFWIMNCPDTDGLNDFLQPLNLRVRNLVIFPVNDNNDVSKAEGGNHWSVLVFYREANVFV 120

Query: 121 HHDSNRGMNKYDAKRLYRAVARFMSGDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDS +G+N   AKRLYRAVARFM G +NSTS PSYVEC ESPQQVNG+DCG+YVTAI+R
Sbjct: 121 HHDSCKGVNNSSAKRLYRAVARFM-GVTNSTSEPSYVECSESPQQVNGHDCGLYVTAISR 180

Query: 181 TICRWYE-PRKEI-DRDG-LWFSAVVEEISPSFVANMRREILGLIRSLMAVKEYRPATK 237
           +ICRWYE  RKE    DG LWFSAVV+E++PS VA+MR EILGLIRS MA+KEY+PA+K
Sbjct: 181 SICRWYEQERKEAGGGDGFLWFSAVVKEVTPSSVADMRSEILGLIRSFMALKEYQPASK 238

[Close]

BLAST of Lsi10G013070 vs. TAIR 10
Match: AT5G60190.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 279.6 bits (714), Expect = 2.3e-75
Identity = 134/227 (59.03%), Postives = 167/227 (73.57%), Query Frame = 0


Query: 1   MGKSEGDEKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSTTHPSKDILLSPPSI 60
           MG +  D+KILSY DVVLR+SDLDIL+GP +LNDR+IEFY S+LST H S  I L PPSI
Sbjct: 1   MGNTSDDDKILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSI 60

Query: 61  AFWIMNCPDAESLNDFLKPLNLCDKKLVIFPVNDNNDVTKAEGGNHWSLLAFYREANVFV 120
           AFWI NCPD E L DF+KPLNL DK L+I PVNDN++V  AEGG HWSLL +Y+EAN FV
Sbjct: 61  AFWISNCPDTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFV 120

Query: 121 HHDSNRGMNKYDAKRLYRAVARFMSGDSNSTSNPSYVECIESPQQVNGYDCGVYVTAIAR 180
           HHDS  G+N++ AK+L++AV+ F+     S  + SY EC ++PQQ NGYDCGV++ A AR
Sbjct: 121 HHDSYMGVNRWSAKQLFKAVSPFV-----SNGDASYKECTDTPQQKNGYDCGVFLLATAR 180

Query: 181 TICRWYEPRKEIDRDGLWFSAVVEEISPSFVANMRREILGLIRSLMA 228
            IC W+      +RD LWF A V+E  P  V ++R EIL LI+ LM+
Sbjct: 181 VICEWFSSGGMKNRDELWF-ANVKETVPDLVNHLREEILALIKKLMS 221

[Close]

BLAST of Lsi10G013070 vs. TAIR 10
Match: AT4G33620.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 46.2 bits (108), Expect = 4.2e-05
Identity = 45/209 (21.53%), Postives = 80/209 (38.28%), Query Frame = 0


Query: 16  VVLRKSDLDILSGPYYLNDRIIEFYFSYLSTTHPSKDILLSPPSIAFWIMNC-------- 75
           VV+RK D+++L    ++ND II+FY  YL      K+         F   NC        
Sbjct: 305 VVVRKQDIELLKPRRFINDTIIDFYIKYLKNRISPKE------RGRFHFFNCFFFRKLAN 364

Query: 76  -----PDA-------ESLNDFLKPLNLCDKKLVIFPVNDNNDVTKAEGGNHWSLLAFYRE 135
                P         + +  + K ++L +K  +  P+N +          HWSL+     
Sbjct: 365 LDKGTPSTCGGREAYQRVQKWTKNVDLFEKDYIFIPINCS---------FHWSLVIICHP 424

Query: 136 ANV----------------------FVHHDSNRGMNKYDAKRLYRAVAR--FMSGDSNST 176
             +                       +H DS +G +K     ++ +  R  + +   N+T
Sbjct: 425 GELVPSHVNFHSFDDEVENPQRVPCILHLDSIKGSHKGGLINIFPSYLREEWKARHENTT 484

The following BLAST results are available for this feature:

BLAST of Lsi10G013070 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSS7	3.2e-74	59.03	NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1	[more]
Q54XR2	5.5e-26	31.82	Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=sen...	[more]
Q96LD8	2.7e-25	30.63	Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1	[more]
Q9D2Z4	1.4e-24	30.84	Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2	[more]
Q5FVJ8	1.8e-24	31.08	Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1	[more]

BLAST of Lsi10G013070 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CSG4	1.7e-118	86.44	NEDD8-specific protease 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3CK81	1.7e-118	86.44	NEDD8-specific protease 1 OS=Cucumis melo OX=3656 GN=LOC103501907 PE=3 SV=1	[more]
A0A0A0KEM9	3.2e-117	86.44	ULP_PROTEASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G28972...	[more]
A0A6J1CWB2	3.7e-110	80.93	NEDD8-specific protease 1 OS=Momordica charantia OX=3673 GN=LOC111015386 PE=3 SV...	[more]
A0A6J1KYI9	1.2e-103	79.92	NEDD8-specific protease 1 OS=Cucurbita maxima OX=3661 GN=LOC111499890 PE=3 SV=1	[more]

BLAST of Lsi10G013070 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038902669.1	7.7e-126	91.95	NEDD8-specific protease 1 [Benincasa hispida]	[more]
XP_008463889.1	3.5e-118	86.44	PREDICTED: NEDD8-specific protease 1 [Cucumis melo] >KAA0035294.1 NEDD8-specific...	[more]
XP_004149559.1	6.5e-117	86.44	NEDD8-specific protease 1 [Cucumis sativus] >XP_031742683.1 NEDD8-specific prote...	[more]
XP_022146090.1	7.7e-110	80.93	NEDD8-specific protease 1 [Momordica charantia]	[more]
XP_023007377.1	2.4e-103	79.92	NEDD8-specific protease 1 [Cucurbita maxima]	[more]

BLAST of Lsi10G013070 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G60190.1	2.3e-75	59.03	Cysteine proteinases superfamily protein	[more]
AT4G33620.1	4.2e-05	21.53	Cysteine proteinases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.395.10	Adenoviral Proteinase; Chain A	coord: 7..228 e-value: 3.7E-63 score: 215.1
None	No IPR available	PANTHER	PTHR46468:SF4	BNAA10G29630D PROTEIN	coord: 2..227
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PFAM	PF02902	Peptidase_C48	coord: 31..182 e-value: 2.3E-13 score: 50.5
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PROSITE	PS50600	ULP_PROTEASE	coord: 16..182 score: 14.719294
IPR044613	NEDD8-specific protease 1/2-like	PANTHER	PTHR46468	SENTRIN-SPECIFIC PROTEASE 8	coord: 2..227
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 7..226

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G013070.1	Lsi10G013070.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0019784	NEDD8-specific protease activity
molecular_function	GO:0008234	cysteine-type peptidase activity