Lsi10G012930 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi10G012930
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	RING/U-box protein
Location	chr10: 17148832 .. 17151688 (-)
RNA-Seq Expression	Lsi10G012930
Synteny	Lsi10G012930

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTGAGTCATTTGAAAGTTTAAAGAGCCGCCAACTGGAATCGGACCCACCATAGAGCAGCGTTTCGCGTCGGAATCCATGGCGGCCGGATTAGTGAACATCGATTCCGACTTAGACGACGATGAACTCGCCTACGACTTGCAAATGGAAGAAGTCATTGCCGCTTCACTGAATCTCCTCCCCTCTACTTCCCACTCTCCTCCACCATCGACTTCTTCGCGACCATCTCCATCTCTCGACGACGATTACGAGTATCACGATGCGGATATGTTACCGGACAACGACGCTTTGGAGGACGAGGTCCTGTTGGACCATCGGCTTCATGACCTCGTCGCCGGAGACGTTATCAACGTCTCCGACGACGAGTGGGAGGAGTACGATTGGCTGTCCTACCGTACGAGCATATCTGCTCTCGATGGTAATCCTTTTTTTGATTCCGAAGGTTTCAGATTGTATTTCAAGGGTTCGGGTAGCGAAGAGACTGTGGGAGACGTAACAGTGATTGTCGTGGCTGTTTTCGATCCGAGACATAATTTATTGGTAGAAGTGAGAAAGCCATTGGAAACTGTTAGAGAATGCGAGGCTATCAATCCCGTGATTTATGAGCTTATGGCTTTAATCGAAGGGCTTGAGGTGGCTCTTGTCTTTCCCTTGGAAAAAGTTTCCTTCTTCTGCGATGATCGTACGCTTTACCGATATGTAAGTTAGCATAATCATGCATTTTCAAGATCTTTGGACATTTCGAACAAAAAGTTGTCTATTCTGATGTCTCAGATTTTTCAACAATTGGATAGTTGCTAAGATAGTTAGTATTCGATTACTTCATATGATATTTGATTCTGCCCCTTTCATTGTACATTGGCGACCCACCATTTGAACACTCAACTAGAAAATTCTCTGTTCATCGCTTTTTTTAATTGTAGAACTGTATGCTACTGTTGTTGTCTTTTGATGCTAATTCTGTTATTATCCTTCAAGCCTCCCTATGATTCCTTTGTTGGACTGTTTCGAGCCATGATTCTGTTCAATTTAAGATTCCGTAGCAGAATTATTTAGTGCTATATGTGATTACTGAATGCAGATTACTGGCAGATTAAGGCCAAGACTGAGAGAGGTTGCAAGATTAGTCGACCAAGTATCTCTTCTACGAGGGCACTTCACTTATTGTGAACCCTTTTTGGTAGAACGAAATGATGTAAAATTTGCCTTTAGATTAGCAAAAGAAGCTACGATTAGCTTGCCTGCTGTAGAAACTGCGCACAGCAGGCAGTTGCTAGAGAACTGCAAGATTTGTTATGAAGATATCGAATTGGATCAAATGTTTACAGTCGATGGCAGCCTTCACAGATACTGTTCTTCTTGCATGAAAAAGCACGTTGAAGTGAAGTTTCTTGGTGGATCTGAGGCAAAATGTCCTCATGAAGGCTGTGAATCTACAATGAGCTTTGAGAGTTGTGATAAGCTTCTCCCACCCAATGTAATTGAAATCATACAACAACGCTTAAAGGAATCTTCCATCCCTTTTTCAGAGAAAGTTTATTGCCCACAACCCAGATGCTCTGCTTTGATGTCAAAAACTGAAGTTTTGGAGTACACAAAAGACACTCATAAGAATGCAGAGCAATCTGGCGCCAGGAAATGCATGAAATGCCACCAACTTTTTTGCATCAAATGCAAAATCTCCTGGCATAAAAGTATGACTTGTGAGGCCTGCTGGGAGTTAAGCTATGACACTCAAACAGCAGATGCGAAGCTGAAGACTCTGGCCAGTGAGAAGTTGTGGCAACCATGTGCAAGATGCAGCCATTTGGTTGAACTTGCAGAAGGATGCTACCACATAATTTGCAGGTCTGCTTCCTCAATTCTCCTCCCACTTTTCTGTTTAGTACATGCGTAATCTATTCACTCAAGTTTTTGGATATGTTAAAAATCATTTCAAACACAACTTTAATCACTTAAAATCAATTCAATGTTTAATTTTACACATTAATCGCATTTTTCAGGCTATTAAAATGGATTTTGAATGATTGAAAACATTTTCGAGTCATCTTAAACAAAAATAATTTTAATCATTTTATAATCACTCCCAAACATGCTAGTAGTTTAACATAGTATGAGCATTGGAAGTTTTGAATTCTAAAACTCATTTCTTCTACATTACACACTCAATATTGATAACTACCTACTACGTCCATTGCTGTTTCGAACCTACATTGTAGACAATGATGAGACTTGTTTCGAAAAACTTTCGAAGTGCTTAAAAAAGTAATTTCAAATATTTTGATAGTCATTTCAAACAGGCTCTTATTATGCTTCGAACTTTTGAGTATAGTGATAATTTAACGGTTTTTGTACTCGTGGTTGTTAATGTTGGGGGCTGATTTTCTTCAGATGTGGATATGAGTTTTGCTATTCTTGTGGAGCTGAGTGGGAGAACAAGCAACCAACGTGTTCTTGTCCCATATGGGACGAACAGCACATCATAAATGATGCACTGTAAAGGATCAATGACAATGTCTGCCGGCGGTGACATTTTCTGAGAGTTGCTTGAAGAATTCTGATGGATTCTGGTAAATATTTTTCACTTTCCTATGGAATTAGTTAAGGTTACAGTACCTGCAGCCTGCAAAGTTAAAATTACTTTCAGGGCTGACTTTGTTCTTTCTATTGAATCTTGATAGTTCGTAGACATACTTATGTAGGAGTTAAGCTGGCCTTAAGAATATTTAACAAGAATAAGAGAAGGAAAGTTGGTGTTCTGCTAGTGTTTTTTTTTCTTTCTCGTAGTTCACTTATAATAAGGCTTATTGTTGGTGCATTAATGGTTTAAATTGGGCTATTAGTTCCAGTTGGTGGAA

mRNA sequence

ATTGAGTCATTTGAAAGTTTAAAGAGCCGCCAACTGGAATCGGACCCACCATAGAGCAGCGTTTCGCGTCGGAATCCATGGCGGCCGGATTAGTGAACATCGATTCCGACTTAGACGACGATGAACTCGCCTACGACTTGCAAATGGAAGAAGTCATTGCCGCTTCACTGAATCTCCTCCCCTCTACTTCCCACTCTCCTCCACCATCGACTTCTTCGCGACCATCTCCATCTCTCGACGACGATTACGAGTATCACGATGCGGATATGTTACCGGACAACGACGCTTTGGAGGACGAGGTCCTGTTGGACCATCGGCTTCATGACCTCGTCGCCGGAGACGTTATCAACGTCTCCGACGACGAGTGGGAGGAGTACGATTGGCTGTCCTACCGTACGAGCATATCTGCTCTCGATGGTAATCCTTTTTTTGATTCCGAAGGTTTCAGATTGTATTTCAAGGGTTCGGGTAGCGAAGAGACTGTGGGAGACGTAACAGTGATTGTCGTGGCTGTTTTCGATCCGAGACATAATTTATTGGTAGAAGTGAGAAAGCCATTGGAAACTGTTAGAGAATGCGAGGCTATCAATCCCGTGATTTATGAGCTTATGGCTTTAATCGAAGGGCTTGAGGTGGCTCTTGTCTTTCCCTTGGAAAAAGTTTCCTTCTTCTGCGATGATCGTACGCTTTACCGATATATTACTGGCAGATTAAGGCCAAGACTGAGAGAGGTTGCAAGATTAGTCGACCAAGTATCTCTTCTACGAGGGCACTTCACTTATTGTGAACCCTTTTTGGTAGAACGAAATGATGTAAAATTTGCCTTTAGATTAGCAAAAGAAGCTACGATTAGCTTGCCTGCTGTAGAAACTGCGCACAGCAGGCAGTTGCTAGAGAACTGCAAGATTTGTTATGAAGATATCGAATTGGATCAAATGTTTACAGTCGATGGCAGCCTTCACAGATACTGTTCTTCTTGCATGAAAAAGCACGTTGAAGTGAAGTTTCTTGGTGGATCTGAGGCAAAATGTCCTCATGAAGGCTGTGAATCTACAATGAGCTTTGAGAGTTGTGATAAGCTTCTCCCACCCAATGTAATTGAAATCATACAACAACGCTTAAAGGAATCTTCCATCCCTTTTTCAGAGAAAGTTTATTGCCCACAACCCAGATGCTCTGCTTTGATGTCAAAAACTGAAGTTTTGGAGTACACAAAAGACACTCATAAGAATGCAGAGCAATCTGGCGCCAGGAAATGCATGAAATGCCACCAACTTTTTTGCATCAAATGCAAAATCTCCTGGCATAAAAGTATGACTTGTGAGGCCTGCTGGGAGTTAAGCTATGACACTCAAACAGCAGATGCGAAGCTGAAGACTCTGGCCAGTGAGAAGTTGTGGCAACCATGTGCAAGATGCAGCCATTTGGTTGAACTTGCAGAAGGATGCTACCACATAATTTGCAGATGTGGATATGAGTTTTGCTATTCTTGTGGAGCTGAGTGGGAGAACAAGCAACCAACGTGTTCTTGTCCCATATGGGACGAACAGCACATCATAAATGATGCACTGTAAAGGATCAATGACAATGTCTGCCGGCGGTGACATTTTCTGAGAGTTGCTTGAAGAATTCTGATGGATTCTGGTAAATATTTTTCACTTTCCTATGGAATTAGTTAAGGTTACAGTACCTGCAGCCTGCAAAGTTAAAATTACTTTCAGGGCTGACTTTGTTCTTTCTATTGAATCTTGATAGTTCGTAGACATACTTATGTAGGAGTTAAGCTGGCCTTAAGAATATTTAACAAGAATAAGAGAAGGAAAGTTGGTGTTCTGCTAGTGTTTTTTTTTCTTTCTCGTAGTTCACTTATAATAAGGCTTATTGTTGGTGCATTAATGGTTTAAATTGGGCTATTAGTTCCAGTTGGTGGAA

Coding sequence (CDS)

ATGGCGGCCGGATTAGTGAACATCGATTCCGACTTAGACGACGATGAACTCGCCTACGACTTGCAAATGGAAGAAGTCATTGCCGCTTCACTGAATCTCCTCCCCTCTACTTCCCACTCTCCTCCACCATCGACTTCTTCGCGACCATCTCCATCTCTCGACGACGATTACGAGTATCACGATGCGGATATGTTACCGGACAACGACGCTTTGGAGGACGAGGTCCTGTTGGACCATCGGCTTCATGACCTCGTCGCCGGAGACGTTATCAACGTCTCCGACGACGAGTGGGAGGAGTACGATTGGCTGTCCTACCGTACGAGCATATCTGCTCTCGATGGTAATCCTTTTTTTGATTCCGAAGGTTTCAGATTGTATTTCAAGGGTTCGGGTAGCGAAGAGACTGTGGGAGACGTAACAGTGATTGTCGTGGCTGTTTTCGATCCGAGACATAATTTATTGGTAGAAGTGAGAAAGCCATTGGAAACTGTTAGAGAATGCGAGGCTATCAATCCCGTGATTTATGAGCTTATGGCTTTAATCGAAGGGCTTGAGGTGGCTCTTGTCTTTCCCTTGGAAAAAGTTTCCTTCTTCTGCGATGATCGTACGCTTTACCGATATATTACTGGCAGATTAAGGCCAAGACTGAGAGAGGTTGCAAGATTAGTCGACCAAGTATCTCTTCTACGAGGGCACTTCACTTATTGTGAACCCTTTTTGGTAGAACGAAATGATGTAAAATTTGCCTTTAGATTAGCAAAAGAAGCTACGATTAGCTTGCCTGCTGTAGAAACTGCGCACAGCAGGCAGTTGCTAGAGAACTGCAAGATTTGTTATGAAGATATCGAATTGGATCAAATGTTTACAGTCGATGGCAGCCTTCACAGATACTGTTCTTCTTGCATGAAAAAGCACGTTGAAGTGAAGTTTCTTGGTGGATCTGAGGCAAAATGTCCTCATGAAGGCTGTGAATCTACAATGAGCTTTGAGAGTTGTGATAAGCTTCTCCCACCCAATGTAATTGAAATCATACAACAACGCTTAAAGGAATCTTCCATCCCTTTTTCAGAGAAAGTTTATTGCCCACAACCCAGATGCTCTGCTTTGATGTCAAAAACTGAAGTTTTGGAGTACACAAAAGACACTCATAAGAATGCAGAGCAATCTGGCGCCAGGAAATGCATGAAATGCCACCAACTTTTTTGCATCAAATGCAAAATCTCCTGGCATAAAAGTATGACTTGTGAGGCCTGCTGGGAGTTAAGCTATGACACTCAAACAGCAGATGCGAAGCTGAAGACTCTGGCCAGTGAGAAGTTGTGGCAACCATGTGCAAGATGCAGCCATTTGGTTGAACTTGCAGAAGGATGCTACCACATAATTTGCAGATGTGGATATGAGTTTTGCTATTCTTGTGGAGCTGAGTGGGAGAACAAGCAACCAACGTGTTCTTGTCCCATATGGGACGAACAGCACATCATAAATGATGCACTGTAA

Protein sequence

MAAGLVNIDSDLDDDELAYDLQMEEVIAASLNLLPSTSHSPPPSTSSRPSPSLDDDYEYHDADMLPDNDALEDEVLLDHRLHDLVAGDVINVSDDEWEEYDWLSYRTSISALDGNPFFDSEGFRLYFKGSGSEETVGDVTVIVVAVFDPRHNLLVEVRKPLETVRECEAINPVIYELMALIEGLEVALVFPLEKVSFFCDDRTLYRYITGRLRPRLREVARLVDQVSLLRGHFTYCEPFLVERNDVKFAFRLAKEATISLPAVETAHSRQLLENCKICYEDIELDQMFTVDGSLHRYCSSCMKKHVEVKFLGGSEAKCPHEGCESTMSFESCDKLLPPNVIEIIQQRLKESSIPFSEKVYCPQPRCSALMSKTEVLEYTKDTHKNAEQSGARKCMKCHQLFCIKCKISWHKSMTCEACWELSYDTQTADAKLKTLASEKLWQPCARCSHLVELAEGCYHIICRCGYEFCYSCGAEWENKQPTCSCPIWDEQHIINDAL

Homology

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BLAST of Lsi10G012930 vs. ExPASy Swiss-Prot
Match: P50876 (E3 ubiquitin-protein ligase RNF144A OS=Homo sapiens OX=9606 GN=RNF144A PE=1 SV=2)

HSP 1 Score: 78.6 bits (192), Expect = 2.3e-13
Identity = 47/207 (22.71%), Postives = 88/207 (42.51%), Query Frame = 0


Query: 272 LENCKICYEDIELDQMFTVDGSLHRYCSSCMKKHVEVKFLGGSEA--KCPHEGC--ESTM 331
           L +CK+C  +  ++QM T+      +C+ C+K++VE+    G E    CP   C  +  +
Sbjct: 17  LVSCKLCLGEYPVEQMTTIAQCQCIFCTLCLKQYVELLIKEGLETAISCPDAACPKQGHL 76

Query: 332 SFESCDKLLPPNVIEIIQQRLKESSIPFSE-KVYCPQPRCSALMSKTEVLEYTKDTHKNA 391
                + ++   +++  ++   E  + F   + +CP   C A+    +V           
Sbjct: 77  QENEIECMVAAEIMQRYKKLQFEREVLFDPCRTWCPASTCQAVCQLQDV---------GL 136

Query: 392 EQSGARKCMKCHQLFCIKCKISWHKSMTCEACWELSYDTQTADAKLKTLASEKLWQPCAR 451
           +     +C  C   FC  CK SWH    C     +++      A  K    +   + C +
Sbjct: 137 QTPQPVQCKACRMEFCSTCKASWHPGQGCPETMPITFLPGETSAAFKMEEDDAPIKRCPK 196

Query: 452 CSHLVELAEGCYHIICR-CGYEFCYSC 473
           C   +E  EGC  ++C+ C + FC+ C
Sbjct: 197 CKVYIERDEGCAQMMCKNCKHAFCWYC 214

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BLAST of Lsi10G012930 vs. ExPASy Swiss-Prot
Match: F4KGU4 (ATP-dependent RNA helicase DEAH12, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g10370 PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 6.6e-13
Identity = 79/343 (23.03%), Postives = 133/343 (38.78%), Query Frame = 0


Query: 150  RHNLLVEVRKPLETVRECEAINPVIYELMALIEG--LEVALVFPLEKVSFFCDDRTLYRY 209
            RHNL V +    E + + E    +I  LM   E   LE+ L  P  +         L + 
Sbjct: 1447 RHNLTVRICGTSEKIAKAE--QELIQALMDYHESKQLEIHLRGPEIRPD-------LMKE 1506

Query: 210  ITGRLRPRLREVARLVDQVSL---LRGHFTYCEPFLVERNDV-KFAFRLAKEATISLPAV 269
            +  R  P L+ +   V  V L    R H          R +V K    LA+E +      
Sbjct: 1507 VVKRFGPELQGIKEKVHGVDLKLNTRYHVIQVHGSKEMRQEVQKMVNELAREKSALGEKP 1566

Query: 270  ETAHSRQLLENCKICYEDIELDQMFTVDGSLHRYCSSCMKKHVE--VKFLGGSEAKCPHE 329
            +     ++   C IC    E+D  ++++G  H +C +C+ +  E  ++        C H 
Sbjct: 1567 D-----EIEVECPICLS--EVDDGYSLEGCSHLFCKACLLEQFEASMRNFDAFPILCSHI 1626

Query: 330  GCESTMSFESCDKLLPPNVIEIIQQRLKESSIPFSEKV--YCPQPRCSALMSKTEVLEYT 389
             C + +       LL    ++ +      S +  S+    +C  P C ++          
Sbjct: 1627 DCGAPIVLADMRALLSQEKLDELFSASLSSFVTSSDGKFRFCSTPDCPSVYRVA------ 1686

Query: 390  KDTHKNAEQSGAR-KCMKCHQLFCIKCKISWHKSMTCEACWELSYDTQTADAKLKTLASE 449
                   ++SG    C  CH   C +C + +H  +TCE   +     +  D  LK  A  
Sbjct: 1687 -----GPQESGEPFICGACHSEICTRCHLEYHPLITCERYKKFK---ENPDLSLKDWAKG 1746

Query: 450  KLWQPCARCSHLVELAEGCYHIICRCGYEFCYSCGAEWENKQP 482
            K  + C  C   +E  +GC H+ CRCG   C++C   +  ++P
Sbjct: 1747 KNVKECPICKSTIEKTDGCNHMKCRCGKHICWTCLDVFTQEEP 1759

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BLAST of Lsi10G012930 vs. ExPASy Swiss-Prot
Match: Q94981 (E3 ubiquitin-protein ligase ariadne-1 OS=Drosophila melanogaster OX=7227 GN=ari-1 PE=1 SV=2)

HSP 1 Score: 76.6 bits (187), Expect = 8.6e-13
Identity = 53/224 (23.66%), Postives = 90/224 (40.18%), Query Frame = 0


Query: 262 AVETAHSRQLLENCKICYEDIELDQMFTVDGSLHRYCSSCMKKHVEVKFLG---GSEAKC 321
           A++   SR   E C+IC+  +  D M  ++   HR+C  C  +++  K +    G    C
Sbjct: 120 AIKQKTSRSQCEECEICFSQLPPDSMAGLECG-HRFCMPCWHEYLSTKIVAEGLGQTISC 179

Query: 322 PHEGCESTMSFESCDKLLPPNVIEIIQQRLKESSIPFSEKV--YCPQPRCSALMSKTEVL 381
              GC+  +   +   L+    + +  Q+L  +S     ++  +CP   C+  +      
Sbjct: 180 AAHGCDILVDDVTVANLVTDARVRVKYQQLITNSFVECNQLLRWCPSVDCTYAV------ 239

Query: 382 EYTKDTHKNAEQSGARKCMKCHQLFCIKCKISWHKSMTCEACWELSYDTQTADAKLKTLA 441
                  K       R   KC  +FC  C  +WH  + C   W   +  +  D    +  
Sbjct: 240 -------KVPYAEPRRVHCKCGHVFCFACGENWHDPVKCR--WLKKWIKKCDDDSETSNW 299

Query: 442 SEKLWQPCARCSHLVELAEGCYHIICR---CGYEFCYSCGAEWE 478
                + C RCS  +E   GC H++C+   C  EFC+ C   WE
Sbjct: 300 IAANTKECPRCSVTIEKDGGCNHMVCKNQNCKNEFCWVCLGSWE 327

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BLAST of Lsi10G012930 vs. ExPASy Swiss-Prot
Match: A4IIY1 (Probable E3 ubiquitin-protein ligase RNF144A OS=Xenopus tropicalis OX=8364 GN=rnf144a PE=2 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.1e-12
Identity = 47/207 (22.71%), Postives = 89/207 (43.00%), Query Frame = 0


Query: 272 LENCKICYEDIELDQMFTVDGSLHRYCSSCMKKHVEVKFLGGSEA--KCPHEGC--ESTM 331
           L +CK+C  +  ++QM T+      +C+ C+K++VE+    G E    CP   C     +
Sbjct: 17  LVSCKLCLGEYTVEQMTTIAQCQCIFCTLCLKQYVELLIKEGLETAISCPDASCPKRGHL 76

Query: 332 SFESCDKLLPPNVIEIIQQRLKESSIPFSE-KVYCPQPRCSALMSKTEVLEYTKDTHKNA 391
                + ++   +++  ++   E  I     + +CP   C A+    E         K  
Sbjct: 77  QENEIECMVAAEIMQKYKKLQFEKEILLDPCRTWCPSSSCQAVCKLQE---------KGI 136

Query: 392 EQSGARKCMKCHQLFCIKCKISWHKSMTCEACWELSYDTQTADAKLKTLASEKLWQPCAR 451
           +     +C  C   FC  CK +WH    C     +++    + +  K+L  +   + C +
Sbjct: 137 QNPQLVQCSACDIEFCSACKANWHPGQGCPENMAITFLPGDSSSFFKSLEDDVPIKRCPK 196

Query: 452 CSHLVELAEGCYHIICR-CGYEFCYSC 473
           C   +E  EGC  ++C+ C + FC+ C
Sbjct: 197 CKVYIERDEGCAQMMCKNCKHAFCWYC 214

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BLAST of Lsi10G012930 vs. ExPASy Swiss-Prot
Match: Q22431 (Potential E3 ubiquitin-protein ligase ariadne-2 OS=Caenorhabditis elegans OX=6239 GN=ari-2 PE=3 SV=2)

HSP 1 Score: 75.9 bits (185), Expect = 1.5e-12
Identity = 55/210 (26.19%), Postives = 87/210 (41.43%), Query Frame = 0


Query: 275 CKICYEDIELDQMFTVDGSLHRYCSSCMKKHVEVKFLGG--SEAKCPHEGCESTMSFESC 334
           C +C  D   +      G  H +C  C K HVE +   G  S  +C    CE     E  
Sbjct: 129 CSVCAMDGYTELPHLTCG--HCFCEHCWKSHVESRLSEGVASRIECMESECEVYAPSEFV 188

Query: 335 DKLLPPN-VIEIIQQR--LKESSIPFSEKVYCPQPRCSALMSKTEVLEYTKDTHKNAEQS 394
             ++  + VI++  +R  L++         +C    C  ++  TEV            + 
Sbjct: 189 LSIIKNSPVIKLKYERFLLRDMVNSHPHLKFCVGNECPVIIRSTEV------------KP 248

Query: 395 GARKCMKCHQLFCIKCKISWHKSMTCEACWELSYDTQTADAKLKTLASEKLWQPCARCSH 454
               CM+CH  FC+KC   +H   +CE   +  + T+ AD            + C +C  
Sbjct: 249 KRVTCMQCHTSFCVKCGADYHAPTSCETIKQ--WMTKCADDSETANYISAHTKDCPQCHS 308

Query: 455 LVELAEGCYHIIC-RCGYEFCYSCGAEWEN 479
            +E A GC HI C RC + FC+ C  +W++
Sbjct: 309 CIEKAGGCNHIQCTRCRHHFCWMCFGDWKS 322

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BLAST of Lsi10G012930 vs. ExPASy TrEMBL
Match: A0A0A0KC52 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G290810 PE=3 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 1.3e-226
Identity = 390/499 (78.16%), Postives = 428/499 (85.77%), Query Frame = 0


Query: 1   MAAGLVNIDSDLDDDELAYDLQMEEVIAASLNLLPSTSHSPPPSTSSRPSPSLDDDYEYH 60
           MA+G  NIDSDL+DDELAYDLQMEEVIAASLNLLPSTSHSPPPSTSSRPSP  DDD E H
Sbjct: 1   MASGATNIDSDLNDDELAYDLQMEEVIAASLNLLPSTSHSPPPSTSSRPSPFPDDDSENH 60

Query: 61  DADMLPDNDALEDEVLLDHRLHDLVAGDVINVSDDEWEEYDWLSYRTSISALD-GNPFFD 120
           DA ML D+DA   +  LDHRL+DLVAGD INVSDDEW  YDW+SYRTS+SAL   NP FD
Sbjct: 61  DAVMLSDDDASVGDDGLDHRLNDLVAGDFINVSDDEWGGYDWMSYRTSVSALKLCNPLFD 120

Query: 121 SEGFRLYFKGSGSEETVGDVTVIVVAVFDPRHNLLVEVRKPLETVRECEAINPVIYELMA 180
            EG RLYFKG  +E++V D TV VVAVFDPR NLL+EV+ PLETVRE  AINPV+ ELMA
Sbjct: 121 PEGSRLYFKGLVNEDSVRDETVTVVAVFDPRENLLMEVKSPLETVRERMAINPVVAELMA 180

Query: 181 LIEGLEVALVFPLEKVSFFCDDRTLYRYITGRLRPRLREVARLVDQVSLLRGHFTYCEPF 240
           LIEGLE ALVFPL +VSFFCDDR LYRYITGRLRP+L+EV RLVDQV LLRG+F+YCEPF
Sbjct: 181 LIEGLEAALVFPLRRVSFFCDDRALYRYITGRLRPKLKEVERLVDQVGLLRGYFSYCEPF 240

Query: 241 LVERNDVKFAFRLAKEATISLPAVETAHSRQLLENCKICYEDIELDQMFTVDGSLHRYCS 300
           LVERNDVK+AFRLA       PA+ET  SR+ +ENCKICYED ELDQMFT+D  LHRYC 
Sbjct: 241 LVERNDVKYAFRLAN----CYPAIETKDSRKFVENCKICYEDRELDQMFTIDDCLHRYCF 300

Query: 301 SCMKKHVEVKFLGGSEAKCPHEGCESTMSFESCDKLLPPNVIEIIQQRLKESSIPFSEKV 360
           SC +KHVEVKFLGGS AKCPHEGCES +  ESCDKLLPPNVIEIIQQRLKESSIPFS+KV
Sbjct: 301 SCTRKHVEVKFLGGSVAKCPHEGCESVVKVESCDKLLPPNVIEIIQQRLKESSIPFSDKV 360

Query: 361 YCPQPRCSALMSKTEVLEYTKDTHKNAEQSGARKCMKCHQLFCIKCKISWHKSMTCEACW 420
           YCPQPRCSALMSKTEVLEYTKD H+NAE SG RKC+KCHQLFCIKCK SWH +MTCE   
Sbjct: 361 YCPQPRCSALMSKTEVLEYTKDIHENAEHSGTRKCVKCHQLFCIKCKSSWHVNMTCEVYK 420

Query: 421 ELSYDTQTADAKLKTLASEKLWQPCARCSHLVELAEGCYHIICRCGYEFCYSCGAEWENK 480
           +  ++TQT DAKLK LA EKLW+PCARCSHLVEL+EGCYHIICRCG+EFCYSCGAEW+NK
Sbjct: 421 KSIHNTQTEDAKLKILAREKLWRPCARCSHLVELSEGCYHIICRCGHEFCYSCGAEWKNK 480

Query: 481 QPTCSCPIWDEQHIINDAL 499
           Q TCSCPIWDE++IIND L
Sbjct: 481 QRTCSCPIWDEENIINDEL 495

[Close]

BLAST of Lsi10G012930 vs. ExPASy TrEMBL
Match: A0A5A7T1R8 (E3 ubiquitin-protein ligase dbl4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00560 PE=3 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 8.7e-226
Identity = 388/499 (77.76%), Postives = 431/499 (86.37%), Query Frame = 0


Query: 1   MAAGLVNIDSDLDDDELAYDLQMEEVIAASLNLLPSTSHSPPPSTSSRPSPSLDDDYEYH 60
           MA+G  NIDSDLDDDELAYDLQMEEVIAASLN LPSTSHSPPPSTSSRPSP  DDD E H
Sbjct: 1   MASGATNIDSDLDDDELAYDLQMEEVIAASLNFLPSTSHSPPPSTSSRPSPFPDDDSENH 60

Query: 61  DADMLPDNDALEDEVLLDHRLHDLVAGDVINVSDDEWEEYDWLSYRTSISALD-GNPFFD 120
           DA ML D+DA   +  L+HRL+DLVA D INVSDDEW  YDW+SYRTS+SAL   NP FD
Sbjct: 61  DAIMLLDDDASVGDDGLEHRLNDLVAEDFINVSDDEWGGYDWMSYRTSMSALKLCNPLFD 120

Query: 121 SEGFRLYFKGSGSEETVGDVTVIVVAVFDPRHNLLVEVRKPLETVRECEAINPVIYELMA 180
            +  RLYFKG  +EE+V D TV VVAVFDPR NL++EV++PLETVRE  AI+PV+ ELMA
Sbjct: 121 PDSSRLYFKGLVNEESVRDETVTVVAVFDPRENLMMEVKRPLETVRERGAISPVVAELMA 180

Query: 181 LIEGLEVALVFPLEKVSFFCDDRTLYRYITGRLRPRLREVARLVDQVSLLRGHFTYCEPF 240
           LIEGLE ALVFPL++VSFFCDDR LYRYITGRLRP+LREV RLVDQV LLRG+F+YCEPF
Sbjct: 181 LIEGLEAALVFPLKRVSFFCDDRALYRYITGRLRPKLREVERLVDQVGLLRGYFSYCEPF 240

Query: 241 LVERNDVKFAFRLAKEATISLPAVETAHSRQLLENCKICYEDIELDQMFTVDGSLHRYCS 300
           LVE NDVK+AF LA       PA+ET  SR+ +ENCKICYED ELDQMFT+DG LHRYC 
Sbjct: 241 LVEPNDVKYAFSLAN----GFPAIETGDSRKFVENCKICYEDRELDQMFTIDGCLHRYCF 300

Query: 301 SCMKKHVEVKFLGGSEAKCPHEGCESTMSFESCDKLLPPNVIEIIQQRLKESSIPFSEKV 360
           SCM+KHVEVKFLGGS AKCPHEGCES +  ESCDKLLPPNVIEIIQQRLKESSIPFS+KV
Sbjct: 301 SCMRKHVEVKFLGGSVAKCPHEGCESIVRVESCDKLLPPNVIEIIQQRLKESSIPFSDKV 360

Query: 361 YCPQPRCSALMSKTEVLEYTKDTHKNAEQSGARKCMKCHQLFCIKCKISWHKSMTCEACW 420
           YCPQPRCSALMSKTEVLEYTK+ +KNA+QSG RKC+KCHQLFCIKCK SWH +MTCEA  
Sbjct: 361 YCPQPRCSALMSKTEVLEYTKEIYKNAKQSGTRKCVKCHQLFCIKCKASWHVNMTCEAYK 420

Query: 421 ELSYDTQTADAKLKTLASEKLWQPCARCSHLVELAEGCYHIICRCGYEFCYSCGAEWENK 480
           + S++TQTADAKLK LA EKLW+ CARCSHLVEL+EGCYHIICRCGYEFCY+CGAEW+NK
Sbjct: 421 KSSHNTQTADAKLKILAREKLWRTCARCSHLVELSEGCYHIICRCGYEFCYTCGAEWKNK 480

Query: 481 QPTCSCPIWDEQHIINDAL 499
           QPTCSCPIWDE++IIND L
Sbjct: 481 QPTCSCPIWDEENIINDEL 495

[Close]

BLAST of Lsi10G012930 vs. ExPASy TrEMBL
Match: A0A1S3CKS8 (E3 ubiquitin-protein ligase dbl4-like OS=Cucumis melo OX=3656 GN=LOC103501923 PE=3 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 8.7e-226
Identity = 388/499 (77.76%), Postives = 431/499 (86.37%), Query Frame = 0


Query: 1   MAAGLVNIDSDLDDDELAYDLQMEEVIAASLNLLPSTSHSPPPSTSSRPSPSLDDDYEYH 60
           MA+G  NIDSDLDDDELAYDLQMEEVIAASLN LPSTSHSPPPSTSSRPSP  DDD E H
Sbjct: 1   MASGATNIDSDLDDDELAYDLQMEEVIAASLNFLPSTSHSPPPSTSSRPSPFPDDDSENH 60

Query: 61  DADMLPDNDALEDEVLLDHRLHDLVAGDVINVSDDEWEEYDWLSYRTSISALD-GNPFFD 120
           DA ML D+DA   +  L+HRL+DLVA D INVSDDEW  YDW+SYRTS+SAL   NP FD
Sbjct: 61  DAIMLLDDDASVGDDGLEHRLNDLVAEDFINVSDDEWGGYDWMSYRTSMSALKLCNPLFD 120

Query: 121 SEGFRLYFKGSGSEETVGDVTVIVVAVFDPRHNLLVEVRKPLETVRECEAINPVIYELMA 180
            +  RLYFKG  +EE+V D TV VVAVFDPR NL++EV++PLETVRE  AI+PV+ ELMA
Sbjct: 121 PDSSRLYFKGLVNEESVRDETVTVVAVFDPRENLMMEVKRPLETVRERGAISPVVAELMA 180

Query: 181 LIEGLEVALVFPLEKVSFFCDDRTLYRYITGRLRPRLREVARLVDQVSLLRGHFTYCEPF 240
           LIEGLE ALVFPL++VSFFCDDR LYRYITGRLRP+LREV RLVDQV LLRG+F+YCEPF
Sbjct: 181 LIEGLEAALVFPLKRVSFFCDDRALYRYITGRLRPKLREVERLVDQVGLLRGYFSYCEPF 240

Query: 241 LVERNDVKFAFRLAKEATISLPAVETAHSRQLLENCKICYEDIELDQMFTVDGSLHRYCS 300
           LVE NDVK+AF LA       PA+ET  SR+ +ENCKICYED ELDQMFT+DG LHRYC 
Sbjct: 241 LVEPNDVKYAFSLAN----GFPAIETGDSRKFVENCKICYEDRELDQMFTIDGCLHRYCF 300

Query: 301 SCMKKHVEVKFLGGSEAKCPHEGCESTMSFESCDKLLPPNVIEIIQQRLKESSIPFSEKV 360
           SCM+KHVEVKFLGGS AKCPHEGCES +  ESCDKLLPPNVIEIIQQRLKESSIPFS+KV
Sbjct: 301 SCMRKHVEVKFLGGSVAKCPHEGCESIVRVESCDKLLPPNVIEIIQQRLKESSIPFSDKV 360

Query: 361 YCPQPRCSALMSKTEVLEYTKDTHKNAEQSGARKCMKCHQLFCIKCKISWHKSMTCEACW 420
           YCPQPRCSALMSKTEVLEYTK+ +KNA+QSG RKC+KCHQLFCIKCK SWH +MTCEA  
Sbjct: 361 YCPQPRCSALMSKTEVLEYTKEIYKNAKQSGTRKCVKCHQLFCIKCKASWHVNMTCEAYK 420

Query: 421 ELSYDTQTADAKLKTLASEKLWQPCARCSHLVELAEGCYHIICRCGYEFCYSCGAEWENK 480
           + S++TQTADAKLK LA EKLW+ CARCSHLVEL+EGCYHIICRCGYEFCY+CGAEW+NK
Sbjct: 421 KSSHNTQTADAKLKILAREKLWRTCARCSHLVELSEGCYHIICRCGYEFCYTCGAEWKNK 480

Query: 481 QPTCSCPIWDEQHIINDAL 499
           QPTCSCPIWDE++IIND L
Sbjct: 481 QPTCSCPIWDEENIINDEL 495

[Close]

BLAST of Lsi10G012930 vs. ExPASy TrEMBL
Match: A0A6J1CFU0 (uncharacterized protein LOC111011041 OS=Momordica charantia OX=3673 GN=LOC111011041 PE=3 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 2.1e-187
Identity = 339/504 (67.26%), Postives = 398/504 (78.97%), Query Frame = 0


Query: 1   MAAGLVNIDSDLDDDELAYDLQMEEVIAASLNLLPSTSHSPPPSTSSRPSPSLDD---DY 60
           MAAG    D+DL DDELA+DLQMEE IAASL LLPSTSHSPP S+SSRPS  L D   D 
Sbjct: 1   MAAGATYFDADL-DDELAFDLQMEEAIAASLALLPSTSHSPPSSSSSRPSRFLVDHRADC 60

Query: 61  EYHDADMLPDNDALEDEVLLDHRLHDLVAGDVINVSDDEWEEYDWLSYRTSISALDG--N 120
           E  DA ++ DNDALED      RLH+LVAGD IN+SDDE +++D   Y T +SA+    N
Sbjct: 61  EIPDAILISDNDALED----GGRLHNLVAGDTINISDDERDDFD-SPYGTDVSAVGSSRN 120

Query: 121 PFFDSEGFRLYFKGSGSEETVGDVTVIVVAVFDPRHNLLVEVRKPLETVRECEAINPVIY 180
             F++E  +LYFKG  SE++V D  VIV A+FD R NLL+EV KPLE VRE +A++  + 
Sbjct: 121 ALFNTECCKLYFKGLISEKSVEDAMVIVAAIFDSRDNLLIEVSKPLEAVRESKALSHKLA 180

Query: 181 ELMALIEGLEVALVFPLEKVSFFCDDRTLYRYITGRLRPRLREVARLVDQVSLLRGHFTY 240
            L ALIEGLE ALV PLE+VSFFCDD T+Y+Y++GR+RPR  +VA LV++V+LLRG FTY
Sbjct: 181 YLRALIEGLEAALVLPLERVSFFCDDHTIYKYVSGRVRPRESKVAALVEKVTLLRGKFTY 240

Query: 241 CEPFLVERNDVKFAFRLAKEATIS---LPAVETAHSRQLL--ENCKICYEDIELDQMFTV 300
           CEP L  ++D KFAF+ +K+AT S   L A +T   R+LL  ENCKIC+ED +L QMFTV
Sbjct: 241 CEPILAAKDDEKFAFKFSKDATASQVRLRAAKTGKCRKLLEKENCKICFEDTKLCQMFTV 300

Query: 301 DGSLHRYCSSCMKKHVEVKFLGGSEAKCPHEGCESTMSFESCDKLLPPNVIEIIQQRLKE 360
           DG LHRYC SCMK+H+EVKFL GSEAKCPHEGCES +S ESC+KLLPP VIEII QR KE
Sbjct: 301 DGCLHRYCFSCMKQHIEVKFLAGSEAKCPHEGCESILSIESCNKLLPPEVIEIIIQRFKE 360

Query: 361 SSIPFSEKVYCPQPRCSALMSKTEVLEYTKDTHKNAEQSGARKCMKCHQLFCIKCKISWH 420
           SSIP SEKVYCP+PRCSALMSKTEVLEYTK+ H NA+QSGAR+CMKCH+LFCI CKISWH
Sbjct: 361 SSIPVSEKVYCPRPRCSALMSKTEVLEYTKNMHGNAKQSGARECMKCHRLFCINCKISWH 420

Query: 421 KSMTCEACWELSYDTQTADAKLKTLASEKLWQPCARCSHLVELAEGCYHIICRCGYEFCY 480
             MTC+     SY  QT DAKLK+LA EKLW+PCARCSHLVELAEGCYHIICRCGYEFCY
Sbjct: 421 DGMTCKDYQNSSYKNQTEDAKLKSLAREKLWRPCARCSHLVELAEGCYHIICRCGYEFCY 480

Query: 481 SCGAEWENKQPTCSCPIWDEQHII 495
           +CGAEW+NK+PTC+CPIW E +II
Sbjct: 481 TCGAEWKNKKPTCACPIWHEHNII 498

[Close]

BLAST of Lsi10G012930 vs. ExPASy TrEMBL
Match: A0A6J1L2U7 (probable E3 ubiquitin-protein ligase ARI9 OS=Cucurbita maxima OX=3661 GN=LOC111499905 PE=3 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 7.0e-175
Identity = 317/501 (63.27%), Postives = 372/501 (74.25%), Query Frame = 0


Query: 1   MAAGLVNIDSDLDDDELAYDLQMEEVIAAS-LNLLPSTSHSPPPSTSSRPSPSLDDDYEY 60
           M  G V +DS LDDDELAYDLQ++EVIA S     P TS+SPPPS SSR SP LDDD E 
Sbjct: 1   MVDGTVILDSGLDDDELAYDLQLKEVIALSQTQFSPPTSYSPPPSASSRSSPFLDDDNEM 60

Query: 61  HDADMLPDNDALEDEVLLDHRLHDLVAGDVINVSDDEWEEYDW-LSYRTSISALDGNPFF 120
             A  L                           SDD+   YD+ +   T +SAL  +P F
Sbjct: 61  RYATNL----------------------WTYLTSDDDRMGYDYRMPSDTDVSAL-LDPLF 120

Query: 121 DSEGFRLYFKGSGSEETVGDVTVIVVAVFDPRHNLLVEVRKPLETVRECEAINPVIYELM 180
           DSE +RLYFKG   +ET GDVT+I VAVFD R +LL+ VRKPLE  RE EA++P + ELM
Sbjct: 121 DSESYRLYFKGLVGKETAGDVTIIAVAVFDSRDDLLIHVRKPLEAARENEAVSPEVAELM 180

Query: 181 ALIEGLEVALVFPLEKVSFFCDDRTLYRYITGRLRPRLREVARLVDQVSLLRGHFTYCEP 240
           ALIEGLE A    LE+VSFFCDD  L++Y+TGRLRP+L ++A LV++V+ LR  F+YCEP
Sbjct: 181 ALIEGLEAARSLHLERVSFFCDDYMLHQYVTGRLRPKLSKIASLVEKVAFLRMQFSYCEP 240

Query: 241 FLVERNDVKFAFRLAKEAT---ISLPAVETAHSRQLLENCKICYEDIELDQMFTVDGSLH 300
           FLVE+N +K AF+L ++AT   I+LP  ET+++  LLENCKICY D ELDQM+TV+  LH
Sbjct: 241 FLVEQNVMKSAFKLVEQATASQITLPDAETSNTHHLLENCKICYVDNELDQMYTVNDCLH 300

Query: 301 RYCSSCMKKHVEVKFLGGSEAKCPHEGCESTMSFESCDKLLPPNVIEIIQQRLKESSIPF 360
           RYC SCMKKHVEVKFL GSEAKCPHEGC+ST+  E+ +KL PP VIEII QR KESSIPF
Sbjct: 301 RYCFSCMKKHVEVKFLDGSEAKCPHEGCKSTVGIENFEKLFPPKVIEIIHQRFKESSIPF 360

Query: 361 SEKVYCPQPRCSALMSKTEVLEYTKDTHKNAEQSGARKCMKCHQLFCIKCKISWHKSMTC 420
           SEKVYCPQPRCSALMSKTEVL+YTKD   NAEQSGARKCMKCH LFCIKCK +WH  MTC
Sbjct: 361 SEKVYCPQPRCSALMSKTEVLDYTKDILGNAEQSGARKCMKCHGLFCIKCKATWHNDMTC 420

Query: 421 EACWELSYDTQTADAKLKTLASEKLWQPCARCSHLVELAEGCYHIICRCGYEFCYSCGAE 480
           E   + S+ T T DAKL +LA EKLWQPC RC HLVELAEGC+HI+CRCG+EFCYSCGAE
Sbjct: 421 EEYRKSSHGTHTEDAKLNSLAKEKLWQPCLRCGHLVELAEGCFHIVCRCGHEFCYSCGAE 478

Query: 481 WENKQPTCSCPIWDEQHIIND 497
           W+N+QPTC CP+WDE  I+ND
Sbjct: 481 WKNRQPTCLCPMWDEHLILND 478

[Close]

BLAST of Lsi10G012930 vs. NCBI nr
Match: XP_038902119.1 (probable E3 ubiquitin-protein ligase ARI9 isoform X1 [Benincasa hispida])

HSP 1 Score: 816.2 bits (2107), Expect = 1.5e-232
Identity = 404/477 (84.70%), Postives = 427/477 (89.52%), Query Frame = 0


Query: 1   MAAGLVNIDSDLDDDELAYDLQMEEVIAASLNLLPSTSHSPPPSTSSRPSPSLDDDYEYH 60
           MA G +NIDSDLDDDELAYDLQ+EEVIAASLN L STS  P PSTSSRPSP LDDDY+  
Sbjct: 1   MAVGEMNIDSDLDDDELAYDLQLEEVIAASLNHLSSTSQFPSPSTSSRPSPFLDDDYKNL 60

Query: 61  DADMLPDNDALEDEVLLDHRLHDLVAGDVINVSDDEWEEYDWLSYRTSISALD--GNPFF 120
           DA ML DNDALEDE  LDHRLHDLVAGDVINVSDDEW+EYDWLS+RTS+S LD  GN FF
Sbjct: 61  DAIMLLDNDALEDEDELDHRLHDLVAGDVINVSDDEWDEYDWLSFRTSVSTLDMLGNHFF 120

Query: 121 DSEGFRLYFKGSGSEETVGDVTVIVVAVFDPRHNLLVEVRKPLETVRECEAINPVIYELM 180
           D EGFRLYFKG  SEE+VGDVTVIVVAVFD R+NLL+EVRKPLETVRE EAINPV+ ELM
Sbjct: 121 DPEGFRLYFKGLVSEESVGDVTVIVVAVFDRRNNLLMEVRKPLETVREREAINPVVAELM 180

Query: 181 ALIEGLEVALVFPLEKVSFFCDDRTLYRYITGRLRPRLREVARLVDQVSLLRGHFTYCEP 240
            LIEGLE ALVFPLE+VS FCDDRTLYRY+T RLRPR+ +VARLV++VS LRGHFTYCEP
Sbjct: 181 TLIEGLEAALVFPLERVSIFCDDRTLYRYVTDRLRPRMSKVARLVERVSFLRGHFTYCEP 240

Query: 241 FLVERNDVKFAFRLAKEATISLPAVETAHSRQLLENCKICYEDIELDQMFTVDGSLHRYC 300
            LVERNDVKFAF LA EA     A ET HSRQLLENCKICYED ELDQMFTVDG LHRYC
Sbjct: 241 ILVERNDVKFAFMLANEAIGFRAAAETEHSRQLLENCKICYEDRELDQMFTVDGCLHRYC 300

Query: 301 SSCMKKHVEVKFLGGSEAKCPHEGCESTMSFESCDKLLPPNVIEIIQQRLKESSIPFSEK 360
            SCMKKHVEVKFLGGSEAKCPHEGCEST+S ESCDKLLPPNVIEIIQQRLKESSIPFSEK
Sbjct: 301 FSCMKKHVEVKFLGGSEAKCPHEGCESTVSIESCDKLLPPNVIEIIQQRLKESSIPFSEK 360

Query: 361 VYCPQPRCSALMSKTEVLEYTKDTHKNAEQSGARKCMKCHQLFCIKCKISWHKSMTCEAC 420
           VYCPQPRCSALMSKTEVLEYTKDTH+NAE+SG R CMKCHQLFC+KCKISWH SMTCEA 
Sbjct: 361 VYCPQPRCSALMSKTEVLEYTKDTHENAERSGVRNCMKCHQLFCVKCKISWHNSMTCEAY 420

Query: 421 WELSYDTQTADAKLKTLASEKLWQPCARCSHLVELAEGCYHIICRCGYEFCYSCGAE 476
           W+LS++TQTADAKLKTLA E LWQPCARCSHLVELAEGCYHIICRCGYEFCYSCGAE
Sbjct: 421 WKLSHNTQTADAKLKTLARENLWQPCARCSHLVELAEGCYHIICRCGYEFCYSCGAE 477

[Close]

BLAST of Lsi10G012930 vs. NCBI nr
Match: XP_004149564.1 (ATP-dependent RNA helicase DEAH12, chloroplastic [Cucumis sativus] >KGN47300.1 hypothetical protein Csa_023036 [Cucumis sativus])

HSP 1 Score: 795.4 bits (2053), Expect = 2.8e-226
Identity = 390/499 (78.16%), Postives = 428/499 (85.77%), Query Frame = 0


Query: 1   MAAGLVNIDSDLDDDELAYDLQMEEVIAASLNLLPSTSHSPPPSTSSRPSPSLDDDYEYH 60
           MA+G  NIDSDL+DDELAYDLQMEEVIAASLNLLPSTSHSPPPSTSSRPSP  DDD E H
Sbjct: 1   MASGATNIDSDLNDDELAYDLQMEEVIAASLNLLPSTSHSPPPSTSSRPSPFPDDDSENH 60

Query: 61  DADMLPDNDALEDEVLLDHRLHDLVAGDVINVSDDEWEEYDWLSYRTSISALD-GNPFFD 120
           DA ML D+DA   +  LDHRL+DLVAGD INVSDDEW  YDW+SYRTS+SAL   NP FD
Sbjct: 61  DAVMLSDDDASVGDDGLDHRLNDLVAGDFINVSDDEWGGYDWMSYRTSVSALKLCNPLFD 120

Query: 121 SEGFRLYFKGSGSEETVGDVTVIVVAVFDPRHNLLVEVRKPLETVRECEAINPVIYELMA 180
            EG RLYFKG  +E++V D TV VVAVFDPR NLL+EV+ PLETVRE  AINPV+ ELMA
Sbjct: 121 PEGSRLYFKGLVNEDSVRDETVTVVAVFDPRENLLMEVKSPLETVRERMAINPVVAELMA 180

Query: 181 LIEGLEVALVFPLEKVSFFCDDRTLYRYITGRLRPRLREVARLVDQVSLLRGHFTYCEPF 240
           LIEGLE ALVFPL +VSFFCDDR LYRYITGRLRP+L+EV RLVDQV LLRG+F+YCEPF
Sbjct: 181 LIEGLEAALVFPLRRVSFFCDDRALYRYITGRLRPKLKEVERLVDQVGLLRGYFSYCEPF 240

Query: 241 LVERNDVKFAFRLAKEATISLPAVETAHSRQLLENCKICYEDIELDQMFTVDGSLHRYCS 300
           LVERNDVK+AFRLA       PA+ET  SR+ +ENCKICYED ELDQMFT+D  LHRYC 
Sbjct: 241 LVERNDVKYAFRLAN----CYPAIETKDSRKFVENCKICYEDRELDQMFTIDDCLHRYCF 300

Query: 301 SCMKKHVEVKFLGGSEAKCPHEGCESTMSFESCDKLLPPNVIEIIQQRLKESSIPFSEKV 360
           SC +KHVEVKFLGGS AKCPHEGCES +  ESCDKLLPPNVIEIIQQRLKESSIPFS+KV
Sbjct: 301 SCTRKHVEVKFLGGSVAKCPHEGCESVVKVESCDKLLPPNVIEIIQQRLKESSIPFSDKV 360

Query: 361 YCPQPRCSALMSKTEVLEYTKDTHKNAEQSGARKCMKCHQLFCIKCKISWHKSMTCEACW 420
           YCPQPRCSALMSKTEVLEYTKD H+NAE SG RKC+KCHQLFCIKCK SWH +MTCE   
Sbjct: 361 YCPQPRCSALMSKTEVLEYTKDIHENAEHSGTRKCVKCHQLFCIKCKSSWHVNMTCEVYK 420

Query: 421 ELSYDTQTADAKLKTLASEKLWQPCARCSHLVELAEGCYHIICRCGYEFCYSCGAEWENK 480
           +  ++TQT DAKLK LA EKLW+PCARCSHLVEL+EGCYHIICRCG+EFCYSCGAEW+NK
Sbjct: 421 KSIHNTQTEDAKLKILAREKLWRPCARCSHLVELSEGCYHIICRCGHEFCYSCGAEWKNK 480

Query: 481 QPTCSCPIWDEQHIINDAL 499
           Q TCSCPIWDE++IIND L
Sbjct: 481 QRTCSCPIWDEENIINDEL 495

[Close]

BLAST of Lsi10G012930 vs. NCBI nr
Match: XP_008463910.1 (PREDICTED: E3 ubiquitin-protein ligase dbl4-like [Cucumis melo] >KAA0035309.1 E3 ubiquitin-protein ligase dbl4-like [Cucumis melo var. makuwa] >TYK14327.1 E3 ubiquitin-protein ligase dbl4-like [Cucumis melo var. makuwa])

HSP 1 Score: 792.7 bits (2046), Expect = 1.8e-225
Identity = 388/499 (77.76%), Postives = 431/499 (86.37%), Query Frame = 0


Query: 1   MAAGLVNIDSDLDDDELAYDLQMEEVIAASLNLLPSTSHSPPPSTSSRPSPSLDDDYEYH 60
           MA+G  NIDSDLDDDELAYDLQMEEVIAASLN LPSTSHSPPPSTSSRPSP  DDD E H
Sbjct: 1   MASGATNIDSDLDDDELAYDLQMEEVIAASLNFLPSTSHSPPPSTSSRPSPFPDDDSENH 60

Query: 61  DADMLPDNDALEDEVLLDHRLHDLVAGDVINVSDDEWEEYDWLSYRTSISALD-GNPFFD 120
           DA ML D+DA   +  L+HRL+DLVA D INVSDDEW  YDW+SYRTS+SAL   NP FD
Sbjct: 61  DAIMLLDDDASVGDDGLEHRLNDLVAEDFINVSDDEWGGYDWMSYRTSMSALKLCNPLFD 120

Query: 121 SEGFRLYFKGSGSEETVGDVTVIVVAVFDPRHNLLVEVRKPLETVRECEAINPVIYELMA 180
            +  RLYFKG  +EE+V D TV VVAVFDPR NL++EV++PLETVRE  AI+PV+ ELMA
Sbjct: 121 PDSSRLYFKGLVNEESVRDETVTVVAVFDPRENLMMEVKRPLETVRERGAISPVVAELMA 180

Query: 181 LIEGLEVALVFPLEKVSFFCDDRTLYRYITGRLRPRLREVARLVDQVSLLRGHFTYCEPF 240
           LIEGLE ALVFPL++VSFFCDDR LYRYITGRLRP+LREV RLVDQV LLRG+F+YCEPF
Sbjct: 181 LIEGLEAALVFPLKRVSFFCDDRALYRYITGRLRPKLREVERLVDQVGLLRGYFSYCEPF 240

Query: 241 LVERNDVKFAFRLAKEATISLPAVETAHSRQLLENCKICYEDIELDQMFTVDGSLHRYCS 300
           LVE NDVK+AF LA       PA+ET  SR+ +ENCKICYED ELDQMFT+DG LHRYC 
Sbjct: 241 LVEPNDVKYAFSLAN----GFPAIETGDSRKFVENCKICYEDRELDQMFTIDGCLHRYCF 300

Query: 301 SCMKKHVEVKFLGGSEAKCPHEGCESTMSFESCDKLLPPNVIEIIQQRLKESSIPFSEKV 360
           SCM+KHVEVKFLGGS AKCPHEGCES +  ESCDKLLPPNVIEIIQQRLKESSIPFS+KV
Sbjct: 301 SCMRKHVEVKFLGGSVAKCPHEGCESIVRVESCDKLLPPNVIEIIQQRLKESSIPFSDKV 360

Query: 361 YCPQPRCSALMSKTEVLEYTKDTHKNAEQSGARKCMKCHQLFCIKCKISWHKSMTCEACW 420
           YCPQPRCSALMSKTEVLEYTK+ +KNA+QSG RKC+KCHQLFCIKCK SWH +MTCEA  
Sbjct: 361 YCPQPRCSALMSKTEVLEYTKEIYKNAKQSGTRKCVKCHQLFCIKCKASWHVNMTCEAYK 420

Query: 421 ELSYDTQTADAKLKTLASEKLWQPCARCSHLVELAEGCYHIICRCGYEFCYSCGAEWENK 480
           + S++TQTADAKLK LA EKLW+ CARCSHLVEL+EGCYHIICRCGYEFCY+CGAEW+NK
Sbjct: 421 KSSHNTQTADAKLKILAREKLWRTCARCSHLVELSEGCYHIICRCGYEFCYTCGAEWKNK 480

Query: 481 QPTCSCPIWDEQHIINDAL 499
           QPTCSCPIWDE++IIND L
Sbjct: 481 QPTCSCPIWDEENIINDEL 495

[Close]

BLAST of Lsi10G012930 vs. NCBI nr
Match: XP_022140346.1 (uncharacterized protein LOC111011041 [Momordica charantia])

HSP 1 Score: 665.2 bits (1715), Expect = 4.3e-187
Identity = 339/504 (67.26%), Postives = 398/504 (78.97%), Query Frame = 0


Query: 1   MAAGLVNIDSDLDDDELAYDLQMEEVIAASLNLLPSTSHSPPPSTSSRPSPSLDD---DY 60
           MAAG    D+DL DDELA+DLQMEE IAASL LLPSTSHSPP S+SSRPS  L D   D 
Sbjct: 1   MAAGATYFDADL-DDELAFDLQMEEAIAASLALLPSTSHSPPSSSSSRPSRFLVDHRADC 60

Query: 61  EYHDADMLPDNDALEDEVLLDHRLHDLVAGDVINVSDDEWEEYDWLSYRTSISALDG--N 120
           E  DA ++ DNDALED      RLH+LVAGD IN+SDDE +++D   Y T +SA+    N
Sbjct: 61  EIPDAILISDNDALED----GGRLHNLVAGDTINISDDERDDFD-SPYGTDVSAVGSSRN 120

Query: 121 PFFDSEGFRLYFKGSGSEETVGDVTVIVVAVFDPRHNLLVEVRKPLETVRECEAINPVIY 180
             F++E  +LYFKG  SE++V D  VIV A+FD R NLL+EV KPLE VRE +A++  + 
Sbjct: 121 ALFNTECCKLYFKGLISEKSVEDAMVIVAAIFDSRDNLLIEVSKPLEAVRESKALSHKLA 180

Query: 181 ELMALIEGLEVALVFPLEKVSFFCDDRTLYRYITGRLRPRLREVARLVDQVSLLRGHFTY 240
            L ALIEGLE ALV PLE+VSFFCDD T+Y+Y++GR+RPR  +VA LV++V+LLRG FTY
Sbjct: 181 YLRALIEGLEAALVLPLERVSFFCDDHTIYKYVSGRVRPRESKVAALVEKVTLLRGKFTY 240

Query: 241 CEPFLVERNDVKFAFRLAKEATIS---LPAVETAHSRQLL--ENCKICYEDIELDQMFTV 300
           CEP L  ++D KFAF+ +K+AT S   L A +T   R+LL  ENCKIC+ED +L QMFTV
Sbjct: 241 CEPILAAKDDEKFAFKFSKDATASQVRLRAAKTGKCRKLLEKENCKICFEDTKLCQMFTV 300

Query: 301 DGSLHRYCSSCMKKHVEVKFLGGSEAKCPHEGCESTMSFESCDKLLPPNVIEIIQQRLKE 360
           DG LHRYC SCMK+H+EVKFL GSEAKCPHEGCES +S ESC+KLLPP VIEII QR KE
Sbjct: 301 DGCLHRYCFSCMKQHIEVKFLAGSEAKCPHEGCESILSIESCNKLLPPEVIEIIIQRFKE 360

Query: 361 SSIPFSEKVYCPQPRCSALMSKTEVLEYTKDTHKNAEQSGARKCMKCHQLFCIKCKISWH 420
           SSIP SEKVYCP+PRCSALMSKTEVLEYTK+ H NA+QSGAR+CMKCH+LFCI CKISWH
Sbjct: 361 SSIPVSEKVYCPRPRCSALMSKTEVLEYTKNMHGNAKQSGARECMKCHRLFCINCKISWH 420

Query: 421 KSMTCEACWELSYDTQTADAKLKTLASEKLWQPCARCSHLVELAEGCYHIICRCGYEFCY 480
             MTC+     SY  QT DAKLK+LA EKLW+PCARCSHLVELAEGCYHIICRCGYEFCY
Sbjct: 421 DGMTCKDYQNSSYKNQTEDAKLKSLAREKLWRPCARCSHLVELAEGCYHIICRCGYEFCY 480

Query: 481 SCGAEWENKQPTCSCPIWDEQHII 495
           +CGAEW+NK+PTC+CPIW E +II
Sbjct: 481 TCGAEWKNKKPTCACPIWHEHNII 498

[Close]

BLAST of Lsi10G012930 vs. NCBI nr
Match: KAG7035460.1 (putative E3 ubiquitin-protein ligase ARI9, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 625.2 bits (1611), Expect = 5.0e-175
Identity = 317/501 (63.27%), Postives = 374/501 (74.65%), Query Frame = 0


Query: 1   MAAGLVNIDSDLDDDELAYDLQMEEVIAAS-LNLLPSTSHSPPPSTSSRPSPSLDDDYEY 60
           M  G V +DS LDDDELAYDLQ++EVIA S     P TS+SPPPS SSR SP LDDD E 
Sbjct: 1   MVDGTVILDSGLDDDELAYDLQLKEVIALSQTQFSPPTSYSPPPSASSRSSPFLDDDNEM 60

Query: 61  HDADMLPDNDALEDEVLLDHRLHDLVAGDVINVSDDEWEEYDW-LSYRTSISALDGNPFF 120
             A  L                           SDD+   YD+ +   T +SAL  +P F
Sbjct: 61  RYATNL----------------------WTYLTSDDDGMGYDYRMPSDTDVSAL-LDPLF 120

Query: 121 DSEGFRLYFKGSGSEETVGDVTVIVVAVFDPRHNLLVEVRKPLETVRECEAINPVIYELM 180
           DSE +RLYFKG   +ET GDVT+I VAVFD R +LL+ VRKPLE  RE EA++P + ELM
Sbjct: 121 DSESYRLYFKGLVGKETAGDVTIIAVAVFDSRDDLLIHVRKPLEAARENEAVSPEVAELM 180

Query: 181 ALIEGLEVALVFPLEKVSFFCDDRTLYRYITGRLRPRLREVARLVDQVSLLRGHFTYCEP 240
           ALI+GLE AL   LE+VSFFCDD  L++Y+TGRLRP+L ++A LV++V+ LR  F+YCEP
Sbjct: 181 ALIKGLEAALSLHLERVSFFCDDYMLHQYVTGRLRPKLSKIASLVEKVAFLRVQFSYCEP 240

Query: 241 FLVERNDVKFAFRLAKEAT---ISLPAVETAHSRQLLENCKICYEDIELDQMFTVDGSLH 300
           FLVE+N +K AF+L ++AT   I+LP  ET+++  LLENC+ICY D ELDQM+TV+  LH
Sbjct: 241 FLVEQNVMKSAFKLVEQATASQITLPDAETSNTHDLLENCEICYVDNELDQMYTVNDCLH 300

Query: 301 RYCSSCMKKHVEVKFLGGSEAKCPHEGCESTMSFESCDKLLPPNVIEIIQQRLKESSIPF 360
           RYC SCMKKHVEVKFL GSEAKCPHEGC+ST+  E+ +KL PP VIEII QR KESSIPF
Sbjct: 301 RYCFSCMKKHVEVKFLDGSEAKCPHEGCKSTVGIENFEKLFPPKVIEIIHQRFKESSIPF 360

Query: 361 SEKVYCPQPRCSALMSKTEVLEYTKDTHKNAEQSGARKCMKCHQLFCIKCKISWHKSMTC 420
           SEKVYCPQPRCSALMSKTEVL+YTKD   NAEQSGARKCMKCH LFCIKCK +WH  MTC
Sbjct: 361 SEKVYCPQPRCSALMSKTEVLDYTKDILGNAEQSGARKCMKCHGLFCIKCKATWHNDMTC 420

Query: 421 EACWELSYDTQTADAKLKTLASEKLWQPCARCSHLVELAEGCYHIICRCGYEFCYSCGAE 480
           E   + S+ TQT DAKL +LA EKLWQPC RC HLVELAEGC+HI+CRCG+EFCYSCGAE
Sbjct: 421 EEYRKSSHGTQTEDAKLNSLAKEKLWQPCLRCGHLVELAEGCFHIVCRCGHEFCYSCGAE 478

Query: 481 WENKQPTCSCPIWDEQHIIND 497
           W+N+QPTC CP+WDE  I+ND
Sbjct: 481 WKNRQPTCLCPMWDEHLILND 478

[Close]

BLAST of Lsi10G012930 vs. TAIR 10
Match: AT5G60250.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 333.6 bits (854), Expect = 2.8e-91
Identity = 196/533 (36.77%), Postives = 290/533 (54.41%), Query Frame = 0


Query: 14  DDELAYDLQMEEVIAASLNLLPSTSHSPP----------------PSTSSRPSPSLDDDY 73
           D E A+ LQMEE +AASL+    T   PP                P+ S     S    +
Sbjct: 4   DSEYAFRLQMEEALAASLSSQSRTPQRPPSPPIVATCGVLIVENNPNDSKTAKTSSGYGF 63

Query: 74  EYHDADMLPDNDALEDEVLLDHRLHDLVAGDVINVSDDEWEEYDWLSYRTSISALDGN-- 133
           ++  A    ++ A         + H+ V G    V  DE      L    S S   GN  
Sbjct: 64  DFKRAVGGGNSKA-------KGKTHESVTG----VRTDERNPNIGLGNSRSTSTGHGNTK 123

Query: 134 ---------PFFDSEG----------------FRLYFKGSGSEET-VGDVTVIV----VA 193
                         EG                +RLYFKG  S+E   G +T +V    VA
Sbjct: 124 PLPQETVRPAQLVGEGSSRVNTAAGKINGNLLYRLYFKGLVSDENGKGKMTDVVSGFGVA 183

Query: 194 VFDPRHNLLVEVRKPLETVRECEAINPVIYELMALIEGLEVALVFPLEKVSFFCDDRTLY 253
           + D R NLL E++ PL        ++    EL ALI GL  AL   ++ + FFCD   ++
Sbjct: 184 ICDQRDNLLFEMKGPLID----NGMSRQGAELKALIRGLTEALKLGIKHIVFFCDSYPIF 243

Query: 254 RYITGRLRPRLREVARLVDQVSLLRGHFTYCEPFLVERNDVKFAFRLAKEATISL--PAV 313
           +Y+TG+   + ++++ L+D +  +  HF+  +  LV RNDVKFA++LA+E+ +S+  P  
Sbjct: 244 QYVTGKWMAKQKKISLLLDDLQSIMQHFSSYQHVLVARNDVKFAYKLARESILSMVTPHE 303

Query: 314 ETAHSRQLL-ENCKICYEDIELDQMFTVDGSLHRYCSSCMKKHVEVKFLGGSEAKCPHEG 373
           +   ++ +L E C IC+ DI  + MF+VD   HR+C  C+K+HVEVK L G   KCPH+G
Sbjct: 304 DPRQAKAVLKEECAICFNDIVAEGMFSVDKCRHRFCFQCVKQHVEVKLLHGMAPKCPHDG 363

Query: 374 CESTMSFESCDKLLPPNVIEIIQQRLKESSIPFSEKVYCPQPRCSALMSKTEVLEYTKDT 433
           C+S +  ++C KLL P + ++ QQRL+E++IP +E+VYCP PRCSALMSKT++ E  K  
Sbjct: 364 CKSELVIDACGKLLTPKLSKLWQQRLQENAIPVTERVYCPYPRCSALMSKTKISESAKSL 423

Query: 434 HKNAEQSGARKCMKCHQLFCIKCKISWHKSMTCEACWELSYDTQTADAKLKTLASEKLWQ 493
                +SG R+C++C  LFC+ CK+ WH +++C    +L  +    D KLK+LA+ K+W+
Sbjct: 424 LSLYPKSGVRRCVECRGLFCVDCKVPWHGNLSCTEYKKLHPEPPADDVKLKSLANNKMWR 483

[Close]

BLAST of Lsi10G012930 vs. TAIR 10
Match: AT2G25370.1 (RING/U-box superfamily protein )

HSP 1 Score: 271.2 bits (692), Expect = 1.7e-72
Identity = 147/369 (39.84%), Postives = 222/369 (60.16%), Query Frame = 0


Query: 117 FFDSEGFRLYFKGSGSEETVG-DVTVIV-----VAVFDPRHNLLVEVRKPLETVRECEAI 176
           F D+  +RL FKG  + E V  DV   V     VA+ D + NLL E++   E++R+ E I
Sbjct: 148 FMDNVVYRLCFKGLVNYEAVADDVEKAVKAGFGVAICDEKDNLLHEIK---ESLRDIE-I 207

Query: 177 NPVIYELMALIEGLEVALVFPLEKVSFFCDDRTLYRYITGRLRPRLREVARLVDQVSLLR 236
           +    E+MAL+ GL  +    +  V  +CDD  +Y+ I GR + + +++  LV++V  + 
Sbjct: 208 SRRGVEIMALVRGLSESFDLGMRNVVIYCDDDWIYQSIIGRGKSK-KKIDHLVEEVQGIL 267

Query: 237 GHFTYCEPFLVERNDVKFAFRLAKEATISLPAVETAHSRQLLENCKICYEDIELDQMFTV 296
                 +  LV RNDVKFAFRLA+EA I   +V+   + +  E C I +E+ +++ MF  
Sbjct: 268 EKMACIDAVLVARNDVKFAFRLAREA-IGRNSVDV--NAEQGETCGIFFEETDVEHMFVT 327

Query: 297 DGSLHRYCSSCMKKHVEVKFLGGSEAKCPHEGCESTMSFESCDKLLPPNVIEIIQQRLKE 356
           +  LHR+C  C+K+HV+VK   G+E  C   GC+  ++ E C K+L   +IE+ +Q++KE
Sbjct: 328 EKCLHRHCFPCVKQHVKVKLRSGTEPTCLEYGCKFKLTLERCSKVLTLKLIEMWKQKMKE 387

Query: 357 SSIPFSEKVYCPQPRCSALMSKTEVLEYTKDTHKNAEQSGARKCMKCHQLFCIKCKISWH 416
            SIP +E++YCP P CS LMSKTE+          A+ S  R C+KC  LFCI CK+  H
Sbjct: 388 DSIPAAERIYCPYPNCSMLMSKTEL-------SSEADLSNVRTCVKCCGLFCIDCKVPSH 447

Query: 417 KSMTCEACWELSYDTQTADAKLKTLASEKLWQPCARCSHLVELAEGCYHIICRCGYEFCY 476
             ++ +   +L  D    D KLK+LA++K+W+ C +C H++EL+ GC H+ CRCGYEFCY
Sbjct: 448 TDLSYDDYKKLHPDPLVDDLKLKSLANDKMWRQCVKCRHMIELSHGCNHMTCRCGYEFCY 501

Query: 477 SCGAEWENK 480
            CG EW+ +
Sbjct: 508 ECGIEWQKR 501

[Close]

BLAST of Lsi10G012930 vs. TAIR 10
Match: AT2G25360.1 (RING/U-box superfamily protein )

HSP 1 Score: 270.0 bits (689), Expect = 3.8e-72
Identity = 131/308 (42.53%), Postives = 191/308 (62.01%), Query Frame = 0


Query: 178 MALIEGLEVALVFPLEKVSFFCDDRTLYRYITGRLRPRLREVARLVDQVSLLRGHFTYCE 237
           MALI GL  +    + +V  +CDD+ LY+YI GR + + ++V  LVD+  LL    TY +
Sbjct: 1   MALIRGLRESFDLGIRQVVVYCDDQLLYQYIVGRGKIK-KKVVHLVDEFQLLLEEMTYTD 60

Query: 238 PFLVERNDVKFAFRLAKEATISLPAVETAHSRQLLENCKICYEDIELDQM-FTVDGSLHR 297
             L+  NDV FAF+LA+EA +S   V+        E C IC+E+ E ++M FT +  +HR
Sbjct: 61  ADLIALNDVNFAFKLAREAIVSRDDVKA-------EICSICFEETEGERMFFTTEKCVHR 120

Query: 298 YCSSCMKKHVEVKFLGGSEAKCPHEGCESTMSFESCDKLLPPNVIEIIQQRLKESSIPFS 357
           +C  C+K++VEVK L G+   C  +GC+  ++ ESC K+L   +IE+ +Q++KE SIP +
Sbjct: 121 HCFPCVKQYVEVKLLSGTVPTCLDDGCKFKLTLESCSKVLTLELIEMWKQKMKEDSIPAA 180

Query: 358 EKVYCPQPRCSALMSKTEVLEYTKDTHKNAEQSGARKCMKCHQLFCIKCKISWHKSMTCE 417
           E++YCP P CS LMSKTE+          ++ S  R C+KC  LFCI CK+  H  ++C 
Sbjct: 181 ERIYCPYPNCSMLMSKTEL-------SSESDLSNDRSCVKCCGLFCIDCKVPSHSDLSCA 240

Query: 418 ACWELSYDTQTADAKLKTLASEKLWQPCARCSHLVELAEGCYHIICRCGYEFCYSCGAEW 477
              +L +D    + KLK+LA +K W+ C  C H++EL+  C H+ CRCGY+FCY C  EW
Sbjct: 241 EYKKLHHDPLVDELKLKSLAKDKKWRQCKMCRHMIELSHACNHMTCRCGYQFCYQCEVEW 293

Query: 478 ENKQPTCS 485
           +N Q TCS
Sbjct: 301 KNDQKTCS 293

[Close]

BLAST of Lsi10G012930 vs. TAIR 10
Match: AT5G37560.1 (RING/U-box superfamily protein )

HSP 1 Score: 265.8 bits (678), Expect = 7.1e-71
Identity = 147/393 (37.40%), Postives = 213/393 (54.20%), Query Frame = 0


Query: 104 SYRTSISALDGNPFFDSEGFRLYFKGSGSEETVGDVTVIV-----VAVFDPRHNLLVEVR 163
           S +   S  +     D+  ++LYFKG  S ET  D   IV     VA+ D   NL   + 
Sbjct: 17  SSKVGQSTFETQSCVDNVVYKLYFKGLVSGETATDKKAIVKVGFGVAICDEADNLFYGMN 76

Query: 164 KPLETVRECEAINPVIYELMALIEGLEVALVFPLEKVSFFCDDRTLYRYITGRLRPRLRE 223
           K L        IN    +++ALI GL  ++   ++ V   CDDR +Y+ I GR +P+ + 
Sbjct: 77  KSLYEA----VINREEADILALITGLYESIHRGVKNVVICCDDRQIYQTIIGREKPQQKN 136

Query: 224 VARLVDQVSLLRGHFTYCEPFLVERNDVKFAFRLAKEATISLPAVETAHSRQLLENCKIC 283
           V  L+++V  LRG        LV   D  FA RLA +A      V+    + L   C IC
Sbjct: 137 V-HLLEEVQRLRGRLASTGTVLVATRDDNFALRLAIDA-----LVKATQEKPL--TCSIC 196

Query: 284 YEDIELDQMFTVDGSLHRYCSSCMKKHVEVKFLGGSEAKCPHEGCESTMSFESCDKLLPP 343
            +  + + M   D  LHR+C SC+K+ V+VK   G    C  +GC+S ++ ESC  +L P
Sbjct: 197 SDKTDAEHMLLNDKCLHRHCFSCVKQQVKVKLRSGIVPPCLEDGCKSELTLESCSMVLTP 256

Query: 344 NVIEIIQQRLKESSIPFSEKVYCPQPRCSALMSKTEVLEYTKDTHKNAEQSGARKCMKCH 403
            +IE+ +++++E  IP +EK+YCP   CS LMSKTE+        + AEQS  R C+KC 
Sbjct: 257 KLIEMWKRKMEEDLIPDAEKIYCPYRSCSMLMSKTEL-------SREAEQSNVRACIKCS 316

Query: 404 QLFCIKCKISWHKSMTCEACWELSYDTQTADAKLKTLASEKLWQPCARCSHLVELAEGCY 463
           +LFCI CK+ WH  ++C     +  +    D  LK LA++++W+ C+ C H++EL EGC 
Sbjct: 317 ELFCIDCKVPWHSDLSCADYKRIHSERLVNDMMLKVLANDQMWRQCSECKHMIELTEGCN 376

Query: 464 HIICRCGYEFCYSCGAEWENKQPTCSCPIWDEQ 492
           HI CRCGYEFCY CG +W  K    SC + D +
Sbjct: 377 HITCRCGYEFCYRCGHKW-TKYHHYSCQLMDNR 389

[Close]

BLAST of Lsi10G012930 vs. TAIR 10
Match: AT2G21420.1 (IBR domain containing protein )

HSP 1 Score: 263.1 bits (671), Expect = 4.6e-70
Identity = 152/386 (39.38%), Postives = 215/386 (55.70%), Query Frame = 0


Query: 110 SALDGNPFFDSEGFRLYFKGSGSEETVGDVTVIV-----VAVFDPRHNLLVEVRKPLETV 169
           SA   N F DS  +RL+FKG  S+ET  D+  IV     +A+ D  + LL  ++K L   
Sbjct: 37  SASASNIFADSLVYRLFFKGLVSDETTTDMEEIVKAGFGIAICDEANTLLYNMKKSL--- 96

Query: 170 RECEAINPVIYELMALIEGLEVALVFPLEKVSFFCDDRTLYRYITGRLRPRLREVARLVD 229
              + INP   E+ ALI  L V++   L  V   C D  +++ +TGR +P+ + +  LV+
Sbjct: 97  NGDDVINPEEVEIKALICVLNVSIQMELRNVMICCGDYQIFQILTGRGKPQ-QNIVHLVE 156

Query: 230 QVSLLRGHFTYCEPFLVERNDVKFAFRLAKEATISLPAVETAHSRQLLENCKICYEDIEL 289
           QV  LRG  +  E  LV R DV             + A+E        E C IC E+ + 
Sbjct: 157 QVQHLRGKLSSTEVVLVPRADV------------IILAIEAIGG----ETCCICRENTDA 216

Query: 290 DQMFTVDGSLHRYCSSCMKKHVEVKFLGGSEAKCPHEGCESTMSFESCDKLLPPNVIEII 349
           D+MF  +   HR C SC+ +HV+   L G    C H  C S ++FESC K+L PN+IE  
Sbjct: 217 DRMFFTENCFHRQCFSCVNRHVQRMLLCGISPTCLHFPCNSELTFESCSKVLTPNLIEFW 276

Query: 350 QQRLKESSIPFSEKVYCPQPRCSALMSKTEVLEYTKDTHKNAEQSGARKCMKCHQLFCIK 409
           +++++E  +P ++K+YCP  RCS LMSKT +        +  +QS  R C+KC +LFCI 
Sbjct: 277 KRKIEEDLVPAADKIYCPYRRCSMLMSKTAL-------SRETDQSNVRACIKCCRLFCID 336

Query: 410 CKISWHKSMTCEACWELSYDTQTADAKLKTLASEKLWQPCARCSHLVELAEGCYHIICRC 469
           CK+  H  ++C    +L+ D    D KLK+LA++K W+ C +CS+LVEL EGC HI CRC
Sbjct: 337 CKVPSHAGLSCVDYKKLNPD-PLYDVKLKSLANKKKWRQCVQCSNLVELFEGCNHITCRC 392

Query: 470 GYEFCYSCGAEWENKQPTCSCPIWDE 491
           G+EFCY CG EW   Q  C  P  D+
Sbjct: 397 GFEFCYVCGKEW--NQRGCLSPGCDD 392

The following BLAST results are available for this feature:

BLAST of Lsi10G012930 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P50876	2.3e-13	22.71	E3 ubiquitin-protein ligase RNF144A OS=Homo sapiens OX=9606 GN=RNF144A PE=1 SV=2	[more]
F4KGU4	6.6e-13	23.03	ATP-dependent RNA helicase DEAH12, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
Q94981	8.6e-13	23.66	E3 ubiquitin-protein ligase ariadne-1 OS=Drosophila melanogaster OX=7227 GN=ari-...	[more]
A4IIY1	1.1e-12	22.71	Probable E3 ubiquitin-protein ligase RNF144A OS=Xenopus tropicalis OX=8364 GN=rn...	[more]
Q22431	1.5e-12	26.19	Potential E3 ubiquitin-protein ligase ariadne-2 OS=Caenorhabditis elegans OX=623...	[more]

BLAST of Lsi10G012930 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KC52	1.3e-226	78.16	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G290810 PE=3 SV=1	[more]
A0A5A7T1R8	8.7e-226	77.76	E3 ubiquitin-protein ligase dbl4-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A1S3CKS8	8.7e-226	77.76	E3 ubiquitin-protein ligase dbl4-like OS=Cucumis melo OX=3656 GN=LOC103501923 PE...	[more]
A0A6J1CFU0	2.1e-187	67.26	uncharacterized protein LOC111011041 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]
A0A6J1L2U7	7.0e-175	63.27	probable E3 ubiquitin-protein ligase ARI9 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]

BLAST of Lsi10G012930 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038902119.1	1.5e-232	84.70	probable E3 ubiquitin-protein ligase ARI9 isoform X1 [Benincasa hispida]	[more]
XP_004149564.1	2.8e-226	78.16	ATP-dependent RNA helicase DEAH12, chloroplastic [Cucumis sativus] >KGN47300.1 h...	[more]
XP_008463910.1	1.8e-225	77.76	PREDICTED: E3 ubiquitin-protein ligase dbl4-like [Cucumis melo] >KAA0035309.1 E3...	[more]
XP_022140346.1	4.3e-187	67.26	uncharacterized protein LOC111011041 [Momordica charantia]	[more]
KAG7035460.1	5.0e-175	63.27	putative E3 ubiquitin-protein ligase ARI9, partial [Cucurbita argyrosperma subsp...	[more]

BLAST of Lsi10G012930 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G60250.1	2.8e-91	36.77	zinc finger (C3HC4-type RING finger) family protein	[more]
AT2G25370.1	1.7e-72	39.84	RING/U-box superfamily protein	[more]
AT2G25360.1	3.8e-72	42.53	RING/U-box superfamily protein	[more]
AT5G37560.1	7.1e-71	37.40	RING/U-box superfamily protein	[more]
AT2G21420.1	4.6e-70	39.38	IBR domain containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002867	IBR domain	SMART	SM00647	ibrneu5	coord: 342..415 e-value: 9.1E-15 score: 65.1 coord: 424..485 e-value: 0.0098 score: 24.2
IPR002867	IBR domain	PFAM	PF01485	IBR	coord: 432..483 e-value: 1.8E-6 score: 28.1 coord: 344..415 e-value: 7.5E-12 score: 45.3
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 171..256 e-value: 7.5E-12 score: 45.1
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 123..257 e-value: 5.9E-6 score: 28.3
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 272..355 e-value: 6.9E-15 score: 56.7
None	No IPR available	GENE3D	1.20.120.1750		coord: 413..497 e-value: 1.5E-12 score: 50.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 34..59
None	No IPR available	PANTHER	PTHR11685:SF252	RBR-TYPE E3 UBIQUITIN TRANSFERASE	coord: 82..493
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 430..493
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 273..354
IPR031127	E3 ubiquitin ligase RBR family	PANTHER	PTHR11685	RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING	coord: 82..493
IPR044066	TRIAD supradomain	PROSITE	PS51873	TRIAD	coord: 271..489 score: 21.979738

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G012930.1	Lsi10G012930.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032436	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
biological_process	GO:0000209	protein polyubiquitination
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0000151	ubiquitin ligase complex
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0004842	ubiquitin-protein transferase activity