Lsi10G012760 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi10G012760
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Pyridoxal 5'-phosphate synthase subunit PdxT
Location	chr10: 17014061 .. 17015913 (+)
RNA-Seq Expression	Lsi10G012760
Synteny	Lsi10G012760

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTACATGTATTTTCAGGACAGAAAACTGGTGGACAGGGGCTTGTTGGAGGCCTGGATTGTACCGTTCACAGAAACTTCTTTGGCAGTCAGGTAACTCTCGTTGGTTTCTCATGTTGGGTTGTGATTGTGATTATCAAGATGCACGAGAATAACTGGAGAACTAAAGAAACTTGTCAATTGGCACGAAATGTGTTTAGGAATCAAATTTGCATAAAGCTATCTCTGCTCTTGTGGTGGTAGCAGACATGAATTTTGCCCTTTCAGTCATCCTACGAATTGAATAAGCTTCTGAAGTTTTTTCTGAACCGTAGCATGTATGAAAAATATAGCTATAAAGAATCTGGGGGAGCTAAGATCCTTGTTAATGGCCATTATGGTCCTTTCGGATTTTTGAACTACACTATTTTAAAACCCATTCTGTTTATTGGTCAAATAATGACTTTTCCCCAATGAAAATTTCCATACTTGATTTAAAATCATCCATATAATCAGCAGCATTATGAGATCTGCTTAAATGAAGTTTTAGTTGAAGCATGGAGTTCAGAATGCAGAATCTTCGATGGTTTTGTTTGGACACAAGCTGATTATTCAAATAAATTCTGCTTAGATGTTGATTCCAGATGTTCTGTTTTCCCCCAAACTACACATGACCAGTATTACTAATAAGTATAGCAAGCCTGCGAAGTTCGCTTTATATTCATTATCCATTTCTTGAGTTCAAAATTTTCTAATTTAAGATTATGATTGTCTAGCTTCAAAGCTTTGAGGCAGAGCTCTCAATACCAGAGCTAGTGTCTACCGAAGGTGGTCCGGAAACATTCCGTGGAGTCTTCATTCGAGCTCCTGCGATTCTTGATGTGGGACCAGAAGTCCAAGTGCTGGCTCACTGCCCTGTCTCTTCAAACTCTAACTTGCGTTCAAATTCCTCTGGTGAAGGCAAAGAGGTTTGCTGTTATTTTCTTCTGCTCTAATAGATCTCATATCATCTATACACTACTAGTGCCTCTATTTTGAACACCTAAATTATTGTACTCTGTATGTATGAACCAGAAGTTTCAAACTATATGTGTGTCGGTTGTGAGACCATTCAGTAGAACTACAGTTTCACTGGTATACTGGAGTTTTCTCTTGTCTTGCGTTTGTTTTCCTCCTACCCCTTCACATTGTGGTTTGAATATCTCAGGAAAAAGGCTCAGAAAGTAAAGTGATTGTAGCCGTAAGGCAAGGGAACTTGCTCGCAACTGCCTTCCATCCTGAATTAACATCAGATAATAGATGGTATATTGTAATTTTAACTTGAAATACCACACAGCATATTGTGACGATCATCTCAAGTGAGTTACAACGATGGCTTATGCAGGCACAGTTACTTCCTGAAGATGGCTGGCGATGTTGAAGAATCCTCTAGTAGCATAGCAAGCATAGGAGTTAACAATTCGAGATTCGAAAGATCGAAGATCGACCTTCCGATTTTTATTTAGTTTAAACCAAATGAACCCCTCGTCAACCCAAATCTACATTTCAAGTTTCACTCTAGGTTTAATCTATCTATTCTTCAGTTTGCACCTTGTTTTATCTTCCTTGTCTTAAGTTACAGTATTCTTCTGCTGTATTAATCTCATTTTACTAGCACTGTGTGTAGGAGATGCTGTACATGGAGTTTAATTTGGAAAGATCCATCTCCTTTTGCACTGGATTAATCATTGTTGGAAAACAAACTGCAGTGTTAAATAATTCAAAGTTGTTAGGCTACTAGGAACTTAATAGTTCCTATTAATTTGAATACTTTTATGGATTTGACTTGGGGATTTTTTTAAATGCTACTAACATGCTGATCAGAACAAATTGC

mRNA sequence

ATGGGACAGAAAACTGGTGGACAGGGGCTTGTTGGAGGCCTGGATTGTACCGTTCACAGAAACTTCTTTGGCAGTCAGCTTCAAAGCTTTGAGGCAGAGCTCTCAATACCAGAGCTAGTGTCTACCGAAGGTGGTCCGGAAACATTCCGTGGAGTCTTCATTCGAGCTCCTGCGATTCTTGATGTGGGACCAGAAGTCCAAGTGCTGGCTCACTGCCCTGTCTCTTCAAACTCTAACTTGCGTTCAAATTCCTCTGGTGAAGGCAAAGAGGAAAAAGGCTCAGAAAGTAAAGTGATTGTAGCCGTAAGGCAAGGGAACTTGCTCGCAACTGCCTTCCATCCTGAATTAACATCAGATAATAGATGGCACAGTTACTTCCTGAAGATGGCTGGCGATGTTGAAGAATCCTCTAGTAGCATAGCAAGCATAGGAGTTAACAATTCGAGATTCGAAAGATCGAAGATCGACCTTCCGATTTTTATTTAGTTTAAACCAAATGAACCCCTCGTCAACCCAAATCTACATTTCAAGTTTCACTCTAGGTTTAATCTATCTATTCTTCAGTTTGCACCTTGTTTTATCTTCCTTGTCTTAAGTTACAGTATTCTTCTGCTGTATTAATCTCATTTTACTAGCACTGTGTGTAGGAGATGCTGTACATGGAGTTTAATTTGGAAAGATCCATCTCCTTTTGCACTGGATTAATCATTGTTGGAAAACAAACTGCAGTGTTAAATAATTCAAAGTTGTTAGGCTACTAGGAACTTAATAGTTCCTATTAATTTGAATACTTTTATGGATTTGACTTGGGGATTTTTTTAAATGCTACTAACATGCTGATCAGAACAAATTGC

Coding sequence (CDS)

ATGGGACAGAAAACTGGTGGACAGGGGCTTGTTGGAGGCCTGGATTGTACCGTTCACAGAAACTTCTTTGGCAGTCAGCTTCAAAGCTTTGAGGCAGAGCTCTCAATACCAGAGCTAGTGTCTACCGAAGGTGGTCCGGAAACATTCCGTGGAGTCTTCATTCGAGCTCCTGCGATTCTTGATGTGGGACCAGAAGTCCAAGTGCTGGCTCACTGCCCTGTCTCTTCAAACTCTAACTTGCGTTCAAATTCCTCTGGTGAAGGCAAAGAGGAAAAAGGCTCAGAAAGTAAAGTGATTGTAGCCGTAAGGCAAGGGAACTTGCTCGCAACTGCCTTCCATCCTGAATTAACATCAGATAATAGATGGCACAGTTACTTCCTGAAGATGGCTGGCGATGTTGAAGAATCCTCTAGTAGCATAGCAAGCATAGGAGTTAACAATTCGAGATTCGAAAGATCGAAGATCGACCTTCCGATTTTTATTTAG

Protein sequence

MGQKTGGQGLVGGLDCTVHRNFFGSQLQSFEAELSIPELVSTEGGPETFRGVFIRAPAILDVGPEVQVLAHCPVSSNSNLRSNSSGEGKEEKGSESKVIVAVRQGNLLATAFHPELTSDNRWHSYFLKMAGDVEESSSSIASIGVNNSRFERSKIDLPIFI

Homology

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BLAST of Lsi10G012760 vs. ExPASy Swiss-Prot
Match: Q8LAD0 (Probable pyridoxal 5'-phosphate synthase subunit PDX2 OS=Arabidopsis thaliana OX=3702 GN=PDX2 PE=1 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 2.1e-53
Identity = 107/166 (64.46%), Postives = 133/166 (80.12%), Query Frame = 0


Query: 1   MGQKTGGQGLVGGLDCTVHRNFFGSQLQSFEAELSIPELVSTEGGPETFRGVFIRAPAIL 60
           +GQK GGQ LVGGLDCTVHRNFFGSQ+QSFEA++ +P+L S EGGPET+RGVFIRAPA+L
Sbjct: 89  VGQKEGGQELVGGLDCTVHRNFFGSQIQSFEADILVPQLTSQEGGPETYRGVFIRAPAVL 148

Query: 61  DVGPEVQVLAHCPVSSNSNLRSNSSGEGKEEKG-SESKVIVAVRQGNLLATAFHPELTSD 120
           DVGP+V+VLA  PV SN  L S+S+ + +EE    E+KVIVAV+QGNLLATAFHPELT+D
Sbjct: 149 DVGPDVEVLADYPVPSNKVLYSSSTVQIQEEDALPETKVIVAVKQGNLLATAFHPELTAD 208

Query: 121 NRWHSYFLKMAGDVEE-----SSSSIASIGVNNSRFERSKIDLPIF 161
            RWHSYF+KM  ++E+     SS +I S+G  ++  E +K DLPIF
Sbjct: 209 TRWHSYFIKMTKEIEQGASSSSSKTIVSVGETSAGPEPAKPDLPIF 254

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BLAST of Lsi10G012760 vs. ExPASy Swiss-Prot
Match: Q54J48 (Probable pyridoxal 5'-phosphate synthase subunit pdx2 OS=Dictyostelium discoideum OX=44689 GN=pdx2 PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.2e-21
Identity = 55/134 (41.04%), Postives = 81/134 (60.45%), Query Frame = 0


Query: 2   GQKTGGQGLVGGLDCTVHRNFFGSQLQSFEAELSIPELVSTEGGP----ETFRGVFIRAP 61
           GQK GGQ L+GGLD  + RN+FG Q+ SFE ++++    S         E F  +FIRAP
Sbjct: 118 GQKVGGQSLIGGLDVLISRNYFGRQIDSFETKINLNLKFSKNNNNSILLENFEAIFIRAP 177

Query: 62  AILDV--GPEVQVLAHCPVSSNSNLRSNSSGEGKEEKGSESKVIVAVRQGNLLATAFHPE 121
           AILDV     V+++    V+              ++ G++ KVI AV+Q N++A+ FHPE
Sbjct: 178 AILDVIDKENVEIIGEYIVT--------------KKDGTKEKVITAVKQNNIIASVFHPE 237

Query: 122 LTSDNRWHSYFLKM 130
           LT+DNR+H YF+++
Sbjct: 238 LTNDNRFHQYFVQL 237

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BLAST of Lsi10G012760 vs. ExPASy Swiss-Prot
Match: A0LUK9 (Pyridoxal 5'-phosphate synthase subunit PdxT OS=Acidothermus cellulolyticus (strain ATCC 43068 / 11B) OX=351607 GN=pdxT PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 5.8e-19
Identity = 56/123 (45.53%), Postives = 71/123 (57.72%), Query Frame = 0


Query: 7   GQGLVGGLDCTVHRNFFGSQLQSFEAELSIPELVSTEGGPETFRGVFIRAPAILDVGPEV 66
           GQ  +G LD TV RN FG Q+ SFEA++    L   +GGP   R VFIRAP + + GP+V
Sbjct: 101 GQETLGVLDITVRRNAFGRQVDSFEADI---PLTGLDGGP--LRAVFIRAPWVEETGPDV 160

Query: 67  QVLAHCPVSSNSNLRSNSSGEGKEEKGSESKVIVAVRQGNLLATAFHPELTSDNRWHSYF 126
           +VL   P                   G  +  IVAVRQG +LATAFHPELT+D R H++F
Sbjct: 161 EVLGRVP------------------SGRHAGRIVAVRQGAVLATAFHPELTADRRLHAFF 200

Query: 127 LKM 130
           + M
Sbjct: 221 VAM 200

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BLAST of Lsi10G012760 vs. ExPASy Swiss-Prot
Match: Q6AFB8 (Pyridoxal 5'-phosphate synthase subunit PdxT OS=Leifsonia xyli subsp. xyli (strain CTCB07) OX=281090 GN=pdxT PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 5.8e-19
Identity = 56/121 (46.28%), Postives = 65/121 (53.72%), Query Frame = 0


Query: 7   GQGLVGGLDCTVHRNFFGSQLQSFEAELSIPELVSTEGGPETFRGVFIRAPAILDVGPEV 66
           GQ  +GGLD  V RN FGSQ +SFE +LS P L     G E    VFIRAP +  VGP  
Sbjct: 98  GQRSLGGLDVAVRRNAFGSQAESFETDLSFPTL-----GAEPMHAVFIRAPIVESVGPRA 157

Query: 67  QVLAHCPVSSNSNLRSNSSGEGKEEKGSESKVIVAVRQGNLLATAFHPELTSDNRWHSYF 126
            VLA  P             +G+         +VAV Q NLL TAFHPEL+ D R+H YF
Sbjct: 158 TVLARVP-------------DGR---------VVAVEQDNLLGTAFHPELSGDKRFHEYF 191

Query: 127 L 128
           L
Sbjct: 218 L 191

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BLAST of Lsi10G012760 vs. ExPASy Swiss-Prot
Match: A6WCI3 (Pyridoxal 5'-phosphate synthase subunit PdxT OS=Kineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216) OX=266940 GN=pdxT PE=3 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.3e-18
Identity = 55/120 (45.83%), Postives = 72/120 (60.00%), Query Frame = 0


Query: 8   QGLVGGLDCTVHRNFFGSQLQSFEAELSIPELVSTEGGPETFRGVFIRAPAILDVGPEVQ 67
           Q  +GGLD TV RN FG Q+ S+E +L +PE+  T GGP    G+FIRAP + + G EV+
Sbjct: 100 QQTLGGLDVTVRRNAFGRQVDSWEEDLPLPEI--TRGGPPV-EGIFIRAPWVEEAGEEVR 159

Query: 68  VLAHCPVSSNSNLRSNSSGEGKEEKGSESKVIVAVRQGNLLATAFHPELTSDNRWHSYFL 127
           VLA                  +   G  +  IVAVRQG+LLAT+FHPE+T D+R H YF+
Sbjct: 160 VLA------------------RLGSGPAAGRIVAVRQGDLLATSFHPEITGDDRVHRYFV 198

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BLAST of Lsi10G012760 vs. ExPASy TrEMBL
Match: A0A5D3CR96 (Glutaminase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00240 PE=3 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.6e-78
Identity = 150/160 (93.75%), Postives = 156/160 (97.50%), Query Frame = 0


Query: 2   GQKTGGQGLVGGLDCTVHRNFFGSQLQSFEAELSIPELVSTEGGPETFRGVFIRAPAILD 61
           GQKTGGQGLVGGLDCTVHRNFFGSQLQSFE+ELSIPELVS+EGGPETFRGVFIRAPAILD
Sbjct: 83  GQKTGGQGLVGGLDCTVHRNFFGSQLQSFESELSIPELVSSEGGPETFRGVFIRAPAILD 142

Query: 62  VGPEVQVLAHCPVSSNSNLRSNSSGEGKEEKGSESKVIVAVRQGNLLATAFHPELTSDNR 121
           VGPEVQVLAHCPVSSNSNL S+SSGEGK+EK +ESKVIVAVRQGNLLATAFHPELTSDNR
Sbjct: 143 VGPEVQVLAHCPVSSNSNLHSSSSGEGKKEKDAESKVIVAVRQGNLLATAFHPELTSDNR 202

Query: 122 WHSYFLKMAGDVEESSSSIASIGVNNSRFERSKIDLPIFI 162
           WHSYFLKMAGDVEESSSS+ SIGVNNSRFERSK DLPIFI
Sbjct: 203 WHSYFLKMAGDVEESSSSLESIGVNNSRFERSKSDLPIFI 242

[Close]

BLAST of Lsi10G012760 vs. ExPASy TrEMBL
Match: A0A1S3CLW1 (Glutaminase OS=Cucumis melo OX=3656 GN=LOC103501941 PE=3 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.6e-78
Identity = 150/160 (93.75%), Postives = 156/160 (97.50%), Query Frame = 0


Query: 2   GQKTGGQGLVGGLDCTVHRNFFGSQLQSFEAELSIPELVSTEGGPETFRGVFIRAPAILD 61
           GQKTGGQGLVGGLDCTVHRNFFGSQLQSFE+ELSIPELVS+EGGPETFRGVFIRAPAILD
Sbjct: 91  GQKTGGQGLVGGLDCTVHRNFFGSQLQSFESELSIPELVSSEGGPETFRGVFIRAPAILD 150

Query: 62  VGPEVQVLAHCPVSSNSNLRSNSSGEGKEEKGSESKVIVAVRQGNLLATAFHPELTSDNR 121
           VGPEVQVLAHCPVSSNSNL S+SSGEGK+EK +ESKVIVAVRQGNLLATAFHPELTSDNR
Sbjct: 151 VGPEVQVLAHCPVSSNSNLHSSSSGEGKKEKDAESKVIVAVRQGNLLATAFHPELTSDNR 210

Query: 122 WHSYFLKMAGDVEESSSSIASIGVNNSRFERSKIDLPIFI 162
           WHSYFLKMAGDVEESSSS+ SIGVNNSRFERSK DLPIFI
Sbjct: 211 WHSYFLKMAGDVEESSSSLESIGVNNSRFERSKSDLPIFI 250

[Close]

BLAST of Lsi10G012760 vs. ExPASy TrEMBL
Match: A0A0A0KAR5 (Glutaminase OS=Cucumis sativus OX=3659 GN=Csa_6G139790 PE=3 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 5.7e-78
Identity = 151/160 (94.38%), Postives = 155/160 (96.88%), Query Frame = 0


Query: 2   GQKTGGQGLVGGLDCTVHRNFFGSQLQSFEAELSIPELVSTEGGPETFRGVFIRAPAILD 61
           GQKTGGQGLVGGLDCTVHRNFFGSQLQSFE+ELSIPELVS+EGGPETFRGVFIRAPAILD
Sbjct: 91  GQKTGGQGLVGGLDCTVHRNFFGSQLQSFESELSIPELVSSEGGPETFRGVFIRAPAILD 150

Query: 62  VGPEVQVLAHCPVSSNSNLRSNSSGEGKEEKGSESKVIVAVRQGNLLATAFHPELTSDNR 121
           VGPEVQVLA CPVSSNSNL S+SSGEGK+EK SESKVIVAVRQGNLLATAFHPELTSDNR
Sbjct: 151 VGPEVQVLARCPVSSNSNLHSSSSGEGKKEKDSESKVIVAVRQGNLLATAFHPELTSDNR 210

Query: 122 WHSYFLKMAGDVEESSSSIASIGVNNSRFERSKIDLPIFI 162
           WHSYFLKMAGDVEESSSSI SIGVNNSRFERSK DLPIFI
Sbjct: 211 WHSYFLKMAGDVEESSSSIESIGVNNSRFERSKSDLPIFI 250

[Close]

BLAST of Lsi10G012760 vs. ExPASy TrEMBL
Match: A0A6J1H447 (Glutaminase OS=Cucurbita moschata OX=3662 GN=LOC111459428 PE=3 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 4.8e-77
Identity = 149/160 (93.12%), Postives = 152/160 (95.00%), Query Frame = 0


Query: 2   GQKTGGQGLVGGLDCTVHRNFFGSQLQSFEAELSIPELVSTEGGPETFRGVFIRAPAILD 61
           GQKTGGQ LVGGLDCTVHRNFFGSQLQSFE ELSIPELVSTEGGPETFRGVFIRAPAILD
Sbjct: 91  GQKTGGQELVGGLDCTVHRNFFGSQLQSFETELSIPELVSTEGGPETFRGVFIRAPAILD 150

Query: 62  VGPEVQVLAHCPVSSNSNLRSNSSGEGKEEKGSESKVIVAVRQGNLLATAFHPELTSDNR 121
           VGPEVQVLAHCPVSSNS L SNSSGEGKEEKG+E KVIVAVRQGNLLATAFHPELTSDNR
Sbjct: 151 VGPEVQVLAHCPVSSNSTLHSNSSGEGKEEKGAEDKVIVAVRQGNLLATAFHPELTSDNR 210

Query: 122 WHSYFLKMAGDVEESSSSIASIGVNNSRFERSKIDLPIFI 162
           WHSYFLKMAGDVE SSSSIASIGV+N RFERS IDLPIF+
Sbjct: 211 WHSYFLKMAGDVEGSSSSIASIGVDNLRFERSNIDLPIFL 250

[Close]

BLAST of Lsi10G012760 vs. ExPASy TrEMBL
Match: A0A6J1H276 (Glutaminase OS=Cucurbita moschata OX=3662 GN=LOC111459428 PE=3 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 4.8e-77
Identity = 149/160 (93.12%), Postives = 152/160 (95.00%), Query Frame = 0


Query: 2   GQKTGGQGLVGGLDCTVHRNFFGSQLQSFEAELSIPELVSTEGGPETFRGVFIRAPAILD 61
           GQKTGGQ LVGGLDCTVHRNFFGSQLQSFE ELSIPELVSTEGGPETFRGVFIRAPAILD
Sbjct: 106 GQKTGGQELVGGLDCTVHRNFFGSQLQSFETELSIPELVSTEGGPETFRGVFIRAPAILD 165

Query: 62  VGPEVQVLAHCPVSSNSNLRSNSSGEGKEEKGSESKVIVAVRQGNLLATAFHPELTSDNR 121
           VGPEVQVLAHCPVSSNS L SNSSGEGKEEKG+E KVIVAVRQGNLLATAFHPELTSDNR
Sbjct: 166 VGPEVQVLAHCPVSSNSTLHSNSSGEGKEEKGAEDKVIVAVRQGNLLATAFHPELTSDNR 225

Query: 122 WHSYFLKMAGDVEESSSSIASIGVNNSRFERSKIDLPIFI 162
           WHSYFLKMAGDVE SSSSIASIGV+N RFERS IDLPIF+
Sbjct: 226 WHSYFLKMAGDVEGSSSSIASIGVDNLRFERSNIDLPIFL 265

[Close]

BLAST of Lsi10G012760 vs. NCBI nr
Match: XP_038900301.1 (probable pyridoxal 5'-phosphate synthase subunit PDX2 [Benincasa hispida])

HSP 1 Score: 302.8 bits (774), Expect = 1.8e-78
Identity = 152/160 (95.00%), Postives = 153/160 (95.62%), Query Frame = 0


Query: 2   GQKTGGQGLVGGLDCTVHRNFFGSQLQSFEAELSIPELVSTEGGPETFRGVFIRAPAILD 61
           GQKTGGQGLVGGLDCTVHRNFFGSQLQSFE ELSIPELVS+EGGPETFRGVFIRAPAILD
Sbjct: 91  GQKTGGQGLVGGLDCTVHRNFFGSQLQSFETELSIPELVSSEGGPETFRGVFIRAPAILD 150

Query: 62  VGPEVQVLAHCPVSSNSNLRSNSSGEGKEEKGSESKVIVAVRQGNLLATAFHPELTSDNR 121
           VGPEVQVLAHCPVSSNSNL SNSSGEGKEEK SE KVIVAVRQGNLLATAFHPELTSDNR
Sbjct: 151 VGPEVQVLAHCPVSSNSNLHSNSSGEGKEEKDSERKVIVAVRQGNLLATAFHPELTSDNR 210

Query: 122 WHSYFLKMAGDVEESSSSIASIGVNNSRFERSKIDLPIFI 162
           WHSYFLKM GDVEESSSSIASIGVNNS FERSK DLPIFI
Sbjct: 211 WHSYFLKMVGDVEESSSSIASIGVNNSTFERSKTDLPIFI 250

[Close]

BLAST of Lsi10G012760 vs. NCBI nr
Match: KAA0035336.1 (putative pyridoxal 5'-phosphate synthase subunit PDX2 [Cucumis melo var. makuwa] >TYK14301.1 putative pyridoxal 5'-phosphate synthase subunit PDX2 [Cucumis melo var. makuwa])

HSP 1 Score: 301.2 bits (770), Expect = 5.3e-78
Identity = 150/160 (93.75%), Postives = 156/160 (97.50%), Query Frame = 0


Query: 2   GQKTGGQGLVGGLDCTVHRNFFGSQLQSFEAELSIPELVSTEGGPETFRGVFIRAPAILD 61
           GQKTGGQGLVGGLDCTVHRNFFGSQLQSFE+ELSIPELVS+EGGPETFRGVFIRAPAILD
Sbjct: 83  GQKTGGQGLVGGLDCTVHRNFFGSQLQSFESELSIPELVSSEGGPETFRGVFIRAPAILD 142

Query: 62  VGPEVQVLAHCPVSSNSNLRSNSSGEGKEEKGSESKVIVAVRQGNLLATAFHPELTSDNR 121
           VGPEVQVLAHCPVSSNSNL S+SSGEGK+EK +ESKVIVAVRQGNLLATAFHPELTSDNR
Sbjct: 143 VGPEVQVLAHCPVSSNSNLHSSSSGEGKKEKDAESKVIVAVRQGNLLATAFHPELTSDNR 202

Query: 122 WHSYFLKMAGDVEESSSSIASIGVNNSRFERSKIDLPIFI 162
           WHSYFLKMAGDVEESSSS+ SIGVNNSRFERSK DLPIFI
Sbjct: 203 WHSYFLKMAGDVEESSSSLESIGVNNSRFERSKSDLPIFI 242

[Close]

BLAST of Lsi10G012760 vs. NCBI nr
Match: XP_008463934.1 (PREDICTED: probable pyridoxal 5'-phosphate synthase subunit PDX2 [Cucumis melo])

HSP 1 Score: 301.2 bits (770), Expect = 5.3e-78
Identity = 150/160 (93.75%), Postives = 156/160 (97.50%), Query Frame = 0


Query: 2   GQKTGGQGLVGGLDCTVHRNFFGSQLQSFEAELSIPELVSTEGGPETFRGVFIRAPAILD 61
           GQKTGGQGLVGGLDCTVHRNFFGSQLQSFE+ELSIPELVS+EGGPETFRGVFIRAPAILD
Sbjct: 91  GQKTGGQGLVGGLDCTVHRNFFGSQLQSFESELSIPELVSSEGGPETFRGVFIRAPAILD 150

Query: 62  VGPEVQVLAHCPVSSNSNLRSNSSGEGKEEKGSESKVIVAVRQGNLLATAFHPELTSDNR 121
           VGPEVQVLAHCPVSSNSNL S+SSGEGK+EK +ESKVIVAVRQGNLLATAFHPELTSDNR
Sbjct: 151 VGPEVQVLAHCPVSSNSNLHSSSSGEGKKEKDAESKVIVAVRQGNLLATAFHPELTSDNR 210

Query: 122 WHSYFLKMAGDVEESSSSIASIGVNNSRFERSKIDLPIFI 162
           WHSYFLKMAGDVEESSSS+ SIGVNNSRFERSK DLPIFI
Sbjct: 211 WHSYFLKMAGDVEESSSSLESIGVNNSRFERSKSDLPIFI 250

[Close]

BLAST of Lsi10G012760 vs. NCBI nr
Match: XP_004145736.1 (probable pyridoxal 5'-phosphate synthase subunit PDX2 [Cucumis sativus] >KGN46830.1 hypothetical protein Csa_020959 [Cucumis sativus])

HSP 1 Score: 300.1 bits (767), Expect = 1.2e-77
Identity = 151/160 (94.38%), Postives = 155/160 (96.88%), Query Frame = 0


Query: 2   GQKTGGQGLVGGLDCTVHRNFFGSQLQSFEAELSIPELVSTEGGPETFRGVFIRAPAILD 61
           GQKTGGQGLVGGLDCTVHRNFFGSQLQSFE+ELSIPELVS+EGGPETFRGVFIRAPAILD
Sbjct: 91  GQKTGGQGLVGGLDCTVHRNFFGSQLQSFESELSIPELVSSEGGPETFRGVFIRAPAILD 150

Query: 62  VGPEVQVLAHCPVSSNSNLRSNSSGEGKEEKGSESKVIVAVRQGNLLATAFHPELTSDNR 121
           VGPEVQVLA CPVSSNSNL S+SSGEGK+EK SESKVIVAVRQGNLLATAFHPELTSDNR
Sbjct: 151 VGPEVQVLARCPVSSNSNLHSSSSGEGKKEKDSESKVIVAVRQGNLLATAFHPELTSDNR 210

Query: 122 WHSYFLKMAGDVEESSSSIASIGVNNSRFERSKIDLPIFI 162
           WHSYFLKMAGDVEESSSSI SIGVNNSRFERSK DLPIFI
Sbjct: 211 WHSYFLKMAGDVEESSSSIESIGVNNSRFERSKSDLPIFI 250

[Close]

BLAST of Lsi10G012760 vs. NCBI nr
Match: XP_023534477.1 (probable pyridoxal 5'-phosphate synthase subunit PDX2 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 298.5 bits (763), Expect = 3.4e-77
Identity = 150/160 (93.75%), Postives = 153/160 (95.62%), Query Frame = 0


Query: 2   GQKTGGQGLVGGLDCTVHRNFFGSQLQSFEAELSIPELVSTEGGPETFRGVFIRAPAILD 61
           GQKTGGQ LVGGLDCTVHRNFFGSQLQSFE ELSIPELVSTEGGPETFRGVFIRAPAILD
Sbjct: 91  GQKTGGQELVGGLDCTVHRNFFGSQLQSFETELSIPELVSTEGGPETFRGVFIRAPAILD 150

Query: 62  VGPEVQVLAHCPVSSNSNLRSNSSGEGKEEKGSESKVIVAVRQGNLLATAFHPELTSDNR 121
           VGPEVQVLAHCPVSSNS L SNSSGEGKEEKG+E KVIVAVRQGNLLATAFHPELTSDNR
Sbjct: 151 VGPEVQVLAHCPVSSNSALHSNSSGEGKEEKGAEDKVIVAVRQGNLLATAFHPELTSDNR 210

Query: 122 WHSYFLKMAGDVEESSSSIASIGVNNSRFERSKIDLPIFI 162
           WHSYFLKMAGDVE SSSSIASIGV+NSRFERS IDLPIF+
Sbjct: 211 WHSYFLKMAGDVEGSSSSIASIGVDNSRFERSNIDLPIFL 250

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BLAST of Lsi10G012760 vs. TAIR 10
Match: AT5G60540.1 (pyridoxine biosynthesis 2 )

HSP 1 Score: 209.9 bits (533), Expect = 1.5e-54
Identity = 107/166 (64.46%), Postives = 133/166 (80.12%), Query Frame = 0


Query: 1   MGQKTGGQGLVGGLDCTVHRNFFGSQLQSFEAELSIPELVSTEGGPETFRGVFIRAPAIL 60
           +GQK GGQ LVGGLDCTVHRNFFGSQ+QSFEA++ +P+L S EGGPET+RGVFIRAPA+L
Sbjct: 89  VGQKEGGQELVGGLDCTVHRNFFGSQIQSFEADILVPQLTSQEGGPETYRGVFIRAPAVL 148

Query: 61  DVGPEVQVLAHCPVSSNSNLRSNSSGEGKEEKG-SESKVIVAVRQGNLLATAFHPELTSD 120
           DVGP+V+VLA  PV SN  L S+S+ + +EE    E+KVIVAV+QGNLLATAFHPELT+D
Sbjct: 149 DVGPDVEVLADYPVPSNKVLYSSSTVQIQEEDALPETKVIVAVKQGNLLATAFHPELTAD 208

Query: 121 NRWHSYFLKMAGDVEE-----SSSSIASIGVNNSRFERSKIDLPIF 161
            RWHSYF+KM  ++E+     SS +I S+G  ++  E +K DLPIF
Sbjct: 209 TRWHSYFIKMTKEIEQGASSSSSKTIVSVGETSAGPEPAKPDLPIF 254

The following BLAST results are available for this feature:

BLAST of Lsi10G012760 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LAD0	2.1e-53	64.46	Probable pyridoxal 5'-phosphate synthase subunit PDX2 OS=Arabidopsis thaliana OX...	[more]
Q54J48	1.2e-21	41.04	Probable pyridoxal 5'-phosphate synthase subunit pdx2 OS=Dictyostelium discoideu...	[more]
A0LUK9	5.8e-19	45.53	Pyridoxal 5'-phosphate synthase subunit PdxT OS=Acidothermus cellulolyticus (str...	[more]
Q6AFB8	5.8e-19	46.28	Pyridoxal 5'-phosphate synthase subunit PdxT OS=Leifsonia xyli subsp. xyli (stra...	[more]
A6WCI3	1.3e-18	45.83	Pyridoxal 5'-phosphate synthase subunit PdxT OS=Kineococcus radiotolerans (strai...	[more]

BLAST of Lsi10G012760 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CR96	2.6e-78	93.75	Glutaminase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00240 PE=...	[more]
A0A1S3CLW1	2.6e-78	93.75	Glutaminase OS=Cucumis melo OX=3656 GN=LOC103501941 PE=3 SV=1	[more]
A0A0A0KAR5	5.7e-78	94.38	Glutaminase OS=Cucumis sativus OX=3659 GN=Csa_6G139790 PE=3 SV=1	[more]
A0A6J1H447	4.8e-77	93.13	Glutaminase OS=Cucurbita moschata OX=3662 GN=LOC111459428 PE=3 SV=1	[more]
A0A6J1H276	4.8e-77	93.13	Glutaminase OS=Cucurbita moschata OX=3662 GN=LOC111459428 PE=3 SV=1	[more]

BLAST of Lsi10G012760 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038900301.1	1.8e-78	95.00	probable pyridoxal 5'-phosphate synthase subunit PDX2 [Benincasa hispida]	[more]
KAA0035336.1	5.3e-78	93.75	putative pyridoxal 5'-phosphate synthase subunit PDX2 [Cucumis melo var. makuwa]...	[more]
XP_008463934.1	5.3e-78	93.75	PREDICTED: probable pyridoxal 5'-phosphate synthase subunit PDX2 [Cucumis melo]	[more]
XP_004145736.1	1.2e-77	94.38	probable pyridoxal 5'-phosphate synthase subunit PDX2 [Cucumis sativus] >KGN4683...	[more]
XP_023534477.1	3.4e-77	93.75	probable pyridoxal 5'-phosphate synthase subunit PDX2 isoform X2 [Cucurbita pepo...	[more]

BLAST of Lsi10G012760 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G60540.1	1.5e-54	64.46	pyridoxine biosynthesis 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002161	Pyridoxal 5'-phosphate synthase subunit PdxT/SNO	PFAM	PF01174	SNO	coord: 13..129 e-value: 5.5E-18 score: 65.5
IPR002161	Pyridoxal 5'-phosphate synthase subunit PdxT/SNO	PANTHER	PTHR31559	PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO	coord: 1..145
IPR002161	Pyridoxal 5'-phosphate synthase subunit PdxT/SNO	PROSITE	PS51130	PDXT_SNO_2	coord: 1..131 score: 33.609138
IPR029062	Class I glutamine amidotransferase-like	GENE3D	3.40.50.880		coord: 1..141 e-value: 2.9E-38 score: 133.5
IPR029062	Class I glutamine amidotransferase-like	SUPERFAMILY	52317	Class I glutamine amidotransferase-like	coord: 4..129
None	No IPR available	PANTHER	PTHR31559:SF1	SUBFAMILY NOT NAMED	coord: 1..145

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G012760.1	Lsi10G012760.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006541	glutamine metabolic process
biological_process	GO:0042823	pyridoxal phosphate biosynthetic process
biological_process	GO:0008614	pyridoxine metabolic process
biological_process	GO:0042819	vitamin B6 biosynthetic process
cellular_component	GO:0005829	cytosol
cellular_component	GO:1903600	glutaminase complex
molecular_function	GO:0004359	glutaminase activity