Lsi10G012370 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G012370
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionheavy metal-associated isoprenylated plant protein 3-like
Locationchr10: 16632059 .. 16634042 (+)
RNA-Seq ExpressionLsi10G012370
SyntenyLsi10G012370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATCCACCTCATCCCCCGTCTTAACCGCGTCTTCGCCGCTATATAATACTCTGCACTTCACTCCCATTTCTTTCACAAACCCAACTCAACTCTGCAACCACACAAAACCCAAAATTATAACAAAACTCCATTCCTAATCCTTACAATGGGCGAGGTCACACTCTCATTCTTCTTCTTCCTTTCATCTAATCCTCCCTATTTCTTTTTTTGAATTTCTCACACTCATTTCCCCTGCTTTCTCTCTGTTTACAGAAAAAGAACACCGAACAAGACAGCACCACCGCCGTCTTCAAGATCGATATCCACTGCGAGGGCTGCGCTAACAAAATCAGAAAGTCTGTCCGAGAAATCCCAGGTAGCTATCCATTCCCAATTCGATATCCTATTATGATTCCAAATCTCACAATAACCTTCCCCTGTTCTTCAATTTTTTTAAGGTGTTGACAGAGTCAGAGCAGACTGGGAGGCGAACAAGCTGACTGTGACCGGAAAATTCGACCCGGCCAAGCTCCGAGAGAATCTCGCCAATAAAACAAAGAAGAAAATCGACATTGTATCATCGGACAGCAAGAAAGAAAAGGAGTCGAAGAAACCGGACGATGACAAACAGGATAAGAAAACAGAGGACAAGAAGCCCAAAGATAAAGAGGTGGGTGGGTGAATAAATAGCAACGGCGCATGTTAGAATGATACAACTGTCAACAACTAAGTTAAATGGGGGTCCGTCAAGTCAACTTGTGGGATTTTTTATAAAAAATTATTTTAACTGATTAAATTTAAATTATTCTGACCAGACTCCGGTGACGACGGCGACATTGAAGGTGGAACTGCACTGCCAGGGTTGCATCGAGAAGATTTACAAAGTTGTATCCAGAACAAGGGGTAAGTTTTACTTTTCAATTTTAATTACTGCATCACCAAATTAATCTTCGAATCGATAAAATTAATTAATGGGTTTTTTTCTTTTTTTCTTTTTTCTTTTTCTGGTTACAGGAGTGGAGGATATGGCGATTGAGAGACAAAAGGATTTGGTAATGGTGAAGGGGAAAATGGACGTGAAGGCTCTGATAGAGAATTTGGAAGAGAAACTGAAGCGGAAAGTTGCCGTGGTGGTACCCAAAAAGGACAAAGACGACGGAGCAAAGGGTGGAGACGGCGGCGACAAGAACAAGGGCGGCGGAGAGGTCGCTCAAGAGGGCGGAGTAATGGAAGGAAACCGGCTTGATTACGTGGCAGTTCCGGTTCCGGGTTATGGATATGGGTACGGATACGGATACGGGTACGGGTATGGAAATGGTGGATTTGTTGGACAACCATTGCCTTCGCCTCAACATTTGCTTTCGCCTCAAATGTTTAGCGATGAAAATCCGAATGCTTGCTCTGTTATGTGATTAGTATAGTAATTTTTTTTTATAAATGTAATTTGCCATTGAATTTTGTAAACTTTGTTCCAATTTCTTGGTTAGAGTATTGTAAGAAATTTTCTAAAGAATAATGTAATTTTATTTATTTATCTTATTACATTATGGTAAAATAGTAGTGTTTGACTAATAATAATGGTAATGGTGACTCACGAATCTAAAAAATAATGGTAATAATGAAAAGGGTGCAAGCATGAAATAAAAATAAAATAGAGTTAAAAAAATAAGAAAGAGAAAATGCAAGTTTATAAATAGTTGTATTAAATGTGAATTTAACACCGATTTATGGAAGTAATTGTTGAAATAAAAAGTAGTTATACTAAAAAGGGATAAAGTTGGGATTAAAAGGGAGAAATATTGGGCCAAAATGCATAATCTCAAAGGGACAATGTGATTGGTGGCAATTTTTTTGGTGATGTCCAGGTCAGCAAAGGCTTAGACCCAATATTTCATAAAAAGAAAAGCAAAATTCAAATCAAGCTATGGATTGGATATTGGATGCATTTTTTATGGCATGTGTTTTGCTATAAAAACTTAAAAGGTTATCCAAATATGAGATTAAG

mRNA sequence

CATCCACCTCATCCCCCGTCTTAACCGCGTCTTCGCCGCTATATAATACTCTGCACTTCACTCCCATTTCTTTCACAAACCCAACTCAACTCTGCAACCACACAAAACCCAAAATTATAACAAAACTCCATTCCTAATCCTTACAATGGGCGAGAAAAAGAACACCGAACAAGACAGCACCACCGCCGTCTTCAAGATCGATATCCACTGCGAGGGCTGCGCTAACAAAATCAGAAAGTCTGTCCGAGAAATCCCAGGTAGCTATCCATTCCCAATTCGATATCCTATTATGATTCCAAATCTCACAATAACCTTCCCCTGTTCTTCAATTTTTTTAAGAGTCAGAGCAGACTGGGAGGCGAACAAGCTGACTGTGACCGGAAAATTCGACCCGGCCAAGCTCCGAGAGAATCTCGCCAATAAAACAAAGAAGAAAATCGACATTGTATCATCGGACAGCAAGAAAGAAAAGGAGTCGAAGAAACCGGACGATGACAAACAGGATAAGAAAACAGAGGACAAGAAGCCCAAAGATAAAGAGACTCCGGTGACGACGGCGACATTGAAGGTGGAACTGCACTGCCAGGGTTGCATCGAGAAGATTTACAAAGTTGTATCCAGAACAAGGGGAGTGGAGGATATGGCGATTGAGAGACAAAAGGATTTGGTAATGGTGAAGGGGAAAATGGACGTGAAGGCTCTGATAGAGAATTTGGAAGAGAAACTGAAGCGGAAAGTTGCCGTGGTGGTACCCAAAAAGGACAAAGACGACGGAGCAAAGGGTGGAGACGGCGGCGACAAGAACAAGGGCGGCGGAGAGGTCGCTCAAGAGGGCGGAGTAATGGAAGGAAACCGGCTTGATTACGTGGCAGTTCCGGTTCCGGGTTATGGATATGGGTACGGATACGGATACGGGTACGGGTATGGAAATGGTGGATTTGTTGGACAACCATTGCCTTCGCCTCAACATTTGCTTTCGCCTCAAATGTTTAGCGATGAAAATCCGAATGCTTGCTCTGTTATGTGATTAGTATAGTAATTTTTTTTTATAAATGTAATTTGCCATTGAATTTTGTAAACTTTGTTCCAATTTCTTGGTTAGAGTATTGTAAGAAATTTTCTAAAGAATAATGTAATTTTATTTATTTATCTTATTACATTATGGTAAAATAGTAGTGTTTGACTAATAATAATGGTAATGGTGACTCACGAATCTAAAAAATAATGGTAATAATGAAAAGGGTGCAAGCATGAAATAAAAATAAAATAGAGTTAAAAAAATAAGAAAGAGAAAATGCAAGTTTATAAATAGTTGTATTAAATGTGAATTTAACACCGATTTATGGAAGTAATTGTTGAAATAAAAAGTAGTTATACTAAAAAGGGATAAAGTTGGGATTAAAAGGGAGAAATATTGGGCCAAAATGCATAATCTCAAAGGGACAATGTGATTGGTGGCAATTTTTTTGGTGATGTCCAGGTCAGCAAAGGCTTAGACCCAATATTTCATAAAAAGAAAAGCAAAATTCAAATCAAGCTATGGATTGGATATTGGATGCATTTTTTATGGCATGTGTTTTGCTATAAAAACTTAAAAGGTTATCCAAATATGAGATTAAG

Coding sequence (CDS)

ATGGGCGAGAAAAAGAACACCGAACAAGACAGCACCACCGCCGTCTTCAAGATCGATATCCACTGCGAGGGCTGCGCTAACAAAATCAGAAAGTCTGTCCGAGAAATCCCAGGTAGCTATCCATTCCCAATTCGATATCCTATTATGATTCCAAATCTCACAATAACCTTCCCCTGTTCTTCAATTTTTTTAAGAGTCAGAGCAGACTGGGAGGCGAACAAGCTGACTGTGACCGGAAAATTCGACCCGGCCAAGCTCCGAGAGAATCTCGCCAATAAAACAAAGAAGAAAATCGACATTGTATCATCGGACAGCAAGAAAGAAAAGGAGTCGAAGAAACCGGACGATGACAAACAGGATAAGAAAACAGAGGACAAGAAGCCCAAAGATAAAGAGACTCCGGTGACGACGGCGACATTGAAGGTGGAACTGCACTGCCAGGGTTGCATCGAGAAGATTTACAAAGTTGTATCCAGAACAAGGGGAGTGGAGGATATGGCGATTGAGAGACAAAAGGATTTGGTAATGGTGAAGGGGAAAATGGACGTGAAGGCTCTGATAGAGAATTTGGAAGAGAAACTGAAGCGGAAAGTTGCCGTGGTGGTACCCAAAAAGGACAAAGACGACGGAGCAAAGGGTGGAGACGGCGGCGACAAGAACAAGGGCGGCGGAGAGGTCGCTCAAGAGGGCGGAGTAATGGAAGGAAACCGGCTTGATTACGTGGCAGTTCCGGTTCCGGGTTATGGATATGGGTACGGATACGGATACGGGTACGGGTATGGAAATGGTGGATTTGTTGGACAACCATTGCCTTCGCCTCAACATTTGCTTTCGCCTCAAATGTTTAGCGATGAAAATCCGAATGCTTGCTCTGTTATGTGA

Protein sequence

MGEKKNTEQDSTTAVFKIDIHCEGCANKIRKSVREIPGSYPFPIRYPIMIPNLTITFPCSSIFLRVRADWEANKLTVTGKFDPAKLRENLANKTKKKIDIVSSDSKKEKESKKPDDDKQDKKTEDKKPKDKETPVTTATLKVELHCQGCIEKIYKVVSRTRGVEDMAIERQKDLVMVKGKMDVKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNKGGGEVAQEGGVMEGNRLDYVAVPVPGYGYGYGYGYGYGYGNGGFVGQPLPSPQHLLSPQMFSDENPNACSVM
Homology
BLAST of Lsi10G012370 vs. ExPASy Swiss-Prot
Match: Q9FJH5 (Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana OX=3702 GN=HIPP03 PE=1 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 6.1e-51
Identity = 137/304 (45.07%), Postives = 173/304 (56.91%), Query Frame = 0

Query: 4   KKNTEQDSTTAVFKIDIHCEGCANKIRKSVREIPGSYPFPIRYPIMIPNLTITFPCSSIF 63
           KK  E  S T V K+D+HCEGCA++I K VR   G                         
Sbjct: 18  KKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQG------------------------V 77

Query: 64  LRVRADWEANKLTVTGKFDPAKLRENLANKTKKKIDIVSSDSK--KEKESKKPDDDKQDK 123
             V+++    KLTVTG  DP KLRE L  KTKKK+D+VS   K  KEKE+K  +D+ + K
Sbjct: 78  ETVKSESATGKLTVTGALDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKK 137

Query: 124 KTEDKKP-----KDKETPVTTATLKVELHCQGCIEKIYKVVSRTRGVEDMAIERQKDLVM 183
             E KKP     K KETPVTTA LK+  HCQGCI KI K V++T+GV  + ++++K+L+ 
Sbjct: 138 SEEKKKPDNNDKKPKETPVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLT 197

Query: 184 VKGKMDVKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNKGGG-------EVAQE 243
           VKG MDVK L+E L EKLKR V +V PKK+KD    G + G+K KGGG       +   +
Sbjct: 198 VKGTMDVKKLVEILSEKLKRAVEIVPPKKEKDK-ENGNENGEKKKGGGGDGGGKEKTGNK 257

Query: 244 GGVMEGNRLDYVAVPVPGYGYGYGYGYGYGYGNGGFVGQPLPSPQHLLSPQMFSDENPNA 294
           GG    N ++Y+A   P YGYGY  G  YGY            P    +PQ+FSDENPNA
Sbjct: 258 GGGEGVNMMEYMAAQ-PAYGYGYYPGGPYGY------------PIQAHAPQIFSDENPNA 283

BLAST of Lsi10G012370 vs. ExPASy Swiss-Prot
Match: Q9LZF1 (Heavy metal-associated isoprenylated plant protein 6 OS=Arabidopsis thaliana OX=3702 GN=HIPP06 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 5.4e-31
Identity = 125/403 (31.02%), Postives = 176/403 (43.67%), Query Frame = 0

Query: 2   GEKKNTEQDSTTAVFKIDIHCEGCANKIRKSVREIPGSYPFPIRYPIMIPNLTITFPCSS 61
           GEKK T+   TT V K+D+HCEGC  KI++  +   G                       
Sbjct: 14  GEKKPTDGGITTVVMKLDMHCEGCGKKIKRIFKHFKGVED-------------------- 73

Query: 62  IFLRVRADWEANKLTVTGKFDPAKLRENLANKTKKKIDIVSSDSKKEKES---------- 121
               V+ D+++NKLTV G  DP ++R+ +A+K K+ +++VS+ +  +KE+          
Sbjct: 74  ----VKIDYKSNKLTVIGNVDPVEVRDKVADKIKRPVELVSTVAPPKKETPPSSGGAEKK 133

Query: 122 -----------------KKPDDDKQDKKTEDKKPKDKETP---VTTATLKVELHCQGCIE 181
                            +KP + K++KK E+ + K    P    +T  LK +LHC+GC  
Sbjct: 134 PSPAAEEKPAEKKPAAVEKPGEKKEEKKKEEGEKKASPPPPPKESTVVLKTKLHCEGCEH 193

Query: 182 KIYKVVSRTRGVEDMAIERQKDLVMVKGKMDVKALIENLEEKLKRKVAVVVPKKD----- 241
           KI ++V++ +GV  +AI+  KDLV+VKG +DVK L   L EKLKR V VV  KKD     
Sbjct: 194 KIKRIVNKIKGVNSVAIDSAKDLVIVKGIIDVKQLTPYLNEKLKRTVEVVPAKKDDGAPV 253

Query: 242 -----------------------KDDGAKGGDGGDKNK---------GGGEVAQEGGVME 294
                                  KD G K  DGG + K         GGG    +GG M+
Sbjct: 254 AAAAAAPAGGEKKDKVAGEKKEIKDVGEKKVDGGGEKKKEVAVGGGGGGGGGGGDGGAMD 313

BLAST of Lsi10G012370 vs. ExPASy Swiss-Prot
Match: Q9SJL2 (Heavy metal-associated isoprenylated plant protein 5 OS=Arabidopsis thaliana OX=3702 GN=HIPP05 PE=1 SV=2)

HSP 1 Score: 127.5 bits (319), Expect = 2.5e-28
Identity = 120/362 (33.15%), Postives = 161/362 (44.48%), Query Frame = 0

Query: 15  VFKIDIHCEGCANKIRKSVREIPGSYPFPIRYPIMIPNLTITFPCSSIFLRVRADWEANK 74
           V+K+D+HCEGCA KI++ V+   G                           V AD   NK
Sbjct: 53  VYKVDMHCEGCAKKIKRMVKHFDG------------------------VKDVTADTGGNK 112

Query: 75  LTVTGKFDPAKLRENLANKTKKKIDIVSSDSKKEKESKKPDDDKQ----DKKTEDKKPKD 134
           L V GK DP KL+E L  KTK+K+ + +   K E        +K+    DK+     P  
Sbjct: 113 LLVVGKIDPVKLQEKLEEKTKRKVVLANPPPKVEGPVAAAVGEKKADGGDKEAAPPAPAP 172

Query: 135 KETPVTTATLKVELHCQGCIEKIYKVVSRTRGVEDMAIERQKDLVMVKGKMDVKALIENL 194
                +   LK+ LHC+GCI+KI K++ + +GVE +AI+  KD+V VKG +DVK L+  L
Sbjct: 173 AAPKESVVPLKIRLHCEGCIQKIKKIILKIKGVETVAIDGAKDVVTVKGTIDVKELVPLL 232

Query: 195 EEKLKRKVAVVVPKKDKDDGA--------------------------------------- 254
            +KLKR V  +VP K KDDGA                                       
Sbjct: 233 TKKLKRTVEPLVPAK-KDDGAAENKKTEAAAPDAKKEAPSAGVNEAKKEGSDGGEKKKEV 292

Query: 255 --------KGGDGGDKNK---GGGEVAQEGG-----VMEGNRLDYV---AVPV------P 294
                   +GGDGG+K K    GGE  ++GG     V   N++DY    A P        
Sbjct: 293 GDGGEKKKEGGDGGEKKKEAGDGGEKKKDGGGVPAPVAMVNKMDYYGYSAYPTAPMHWQE 352

BLAST of Lsi10G012370 vs. ExPASy Swiss-Prot
Match: Q9ZUV1 (Heavy metal-associated isoprenylated plant protein 1 OS=Arabidopsis thaliana OX=3702 GN=HIPP01 PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.5e-20
Identity = 96/296 (32.43%), Postives = 143/296 (48.31%), Query Frame = 0

Query: 3   EKKNTEQDSTTA----VFKIDIHCEGCANKIRKSVREIPGSYPFPIRYPIMIPNLTITFP 62
           E+K  +Q++TT+    V KID HC+GC  +I +  R + G                    
Sbjct: 16  EEKKKKQNNTTSPVHVVLKIDFHCDGCIARIVRLSRRLEG-------------------- 75

Query: 63  CSSIFLRVRADWEANKLTVTG-KFDPAKLRENLANKTKKKIDIVSSDSKKEKESKKPDDD 122
                  VRAD ++NKLT+ G   DP K+ E L  K+KKK++++S   K +K++K+ ++ 
Sbjct: 76  ----VETVRADPDSNKLTLIGFIMDPVKIAEKLQKKSKKKVELIS--PKPKKDTKENNEK 135

Query: 123 KQDKKTEDKKPKDKETPVTTATLKVELHCQGCIEKIYKVVSRTRGVEDMAIERQKDLVMV 182
           K + KT+          VTT  LKV   C GCI++I K VS T+GV  + ++++K+ V V
Sbjct: 136 KANDKTQ------TVVAVTTVVLKVNCSCDGCIKRIQKAVSTTKGVYQVKMDKEKETVTV 195

Query: 183 KGKMDVKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNKGGGEVAQEGGVMEGNR 242
            G MD+K++ +NL+ KLK+ V VV  KK K    K  D  + N   G   Q         
Sbjct: 196 MGTMDIKSVTDNLKRKLKKTVQVVPEKKKK---KKDKDNAEVNTKVGSPCQ--------- 245

Query: 243 LDYVAVPVPGYGYGYGYGYGYGYGNGGFVGQPLPSPQHLLSPQMFSDENPNACSVM 294
                   PG G  +G G  Y +  G   G              FS+E+ + CSVM
Sbjct: 256 --------PGNGCTHGIG-PYRFMEGPMTG-------------FFSEEDQSYCSVM 245

BLAST of Lsi10G012370 vs. ExPASy Swiss-Prot
Match: Q9C5D3 (Heavy metal-associated isoprenylated plant protein 7 OS=Arabidopsis thaliana OX=3702 GN=HIPP07 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.9e-15
Identity = 90/319 (28.21%), Postives = 133/319 (41.69%), Query Frame = 0

Query: 15  VFKIDIHCEGCANKIRKSVREIPGSYPFPIRYPIMIPNLTITFPCSSIFLRVRADWEANK 74
           V K+ +HCEGCA K+R+ ++   G                           V  D +  K
Sbjct: 76  VLKVYMHCEGCARKVRRCLKGFEGVED------------------------VMTDCKTGK 135

Query: 75  LTVTG-KFDPAKLRENLANKTKKKIDIVSSDSKKEKESKKPDDDKQDKKTEDKKP----K 134
           + V G K DP K+   +  KT +++ ++S           P     +KK E+ KP    K
Sbjct: 136 VVVKGEKADPLKVLARVQRKTHRQVQLLS--------PIPPPPPPPEKKAEEDKPIVEEK 195

Query: 135 DKETP-VTTATLKVELHCQGCIEKIYKVVSRTRGVEDMAIERQKDLVMVKGKMDVKALIE 194
             E P V T  LKV +HC+ C  +I K + R +GVE    + +   V VKG  + + L+E
Sbjct: 196 KVEPPVVVTVVLKVHMHCEACATEIKKRIMRMKGVESAESDLKSSQVTVKGVFEPQKLVE 255

Query: 195 NLEEKLKRKVAVVV------------------PKKDKDDG---AKGGDG----------- 254
            + ++  +  A++                    KK+++ G   +KG +G           
Sbjct: 256 YVYKRTGKHAAIMKIDPPPPPPPEEAAAAAEGEKKEEEKGEGESKGEEGKDDKAKTDEEK 315

Query: 255 --GDKNKGGGEVAQEGGVMEGNRLDYVAVPVPGYGYGYGYGYGYGYGNGGFVGQPLPSPQ 294
             GD  KG GE A  GG  E  ++  V      Y       Y Y Y        P+  P 
Sbjct: 316 KEGDGGKGEGEAADNGGGEEEGKVVEVRKIENPY-------YYYYYQPPRVAIPPMEMPP 355

BLAST of Lsi10G012370 vs. ExPASy TrEMBL
Match: A0A1S3CP34 (heavy metal-associated isoprenylated plant protein 3-like OS=Cucumis melo OX=3656 GN=LOC103502993 PE=4 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 1.0e-117
Identity = 243/295 (82.37%), Postives = 250/295 (84.75%), Query Frame = 0

Query: 1   MGEKKNTEQDSTTAVFKIDIHCEGCANKIRKSVREIPGSYPFPIRYPIMIPNLTITFPCS 60
           MGEKKN   D TTAVFKIDIHCEGCANK+RKSVREIPG                      
Sbjct: 1   MGEKKN--DDITTAVFKIDIHCEGCANKLRKSVREIPG---------------------- 60

Query: 61  SIFLRVRADWEANKLTVTGKFDPAKLRENLANKTKKKIDIVSSDSKKEKES-KKPDDDKQ 120
               RVRADWEANKLTV GKFD AKLR+NLA+KTKKKIDIVSS+ KKEKES KK DD+K 
Sbjct: 61  --VARVRADWEANKLTVIGKFDAAKLRDNLADKTKKKIDIVSSEGKKEKESAKKQDDEKP 120

Query: 121 DKKTEDKK-PKDKETPVTTATLKVELHCQGCIEKIYKVVSRTRGVEDMAIERQKDLVMVK 180
           DKKTEDKK PKDKE PVTTATLKVELHCQGCIEKIYKVVSRT+GVEDMAIERQKDLVMVK
Sbjct: 121 DKKTEDKKQPKDKEIPVTTATLKVELHCQGCIEKIYKVVSRTKGVEDMAIERQKDLVMVK 180

Query: 181 GKMDVKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNKGGGEVAQEGGVMEGNRL 240
           GKMDVKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNK GGEVAQEGG MEGNRL
Sbjct: 181 GKMDVKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNKTGGEVAQEGGAMEGNRL 240

Query: 241 DYVAVPVPGYGYGYGYGYGYGYGNGGFVGQPLPSPQHLLSPQMFSDENPNACSVM 294
           DYVAVPVP  GYGYGYGYGYGYGNGGFVGQ LP PQ LLSPQMFSDENPNACS+M
Sbjct: 241 DYVAVPVP--GYGYGYGYGYGYGNGGFVGQHLPQPQPLLSPQMFSDENPNACSLM 267

BLAST of Lsi10G012370 vs. ExPASy TrEMBL
Match: A0A0A0KDD7 (HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G147620 PE=4 SV=1)

HSP 1 Score: 420.2 bits (1079), Expect = 6.9e-114
Identity = 236/295 (80.00%), Postives = 248/295 (84.07%), Query Frame = 0

Query: 1   MGEKKNTEQDSTTAVFKIDIHCEGCANKIRKSVREIPGSYPFPIRYPIMIPNLTITFPCS 60
           MGEKKN   D+TTAVFKIDIHCEGCANK+R+SVR+IPG                      
Sbjct: 1   MGEKKN--DDTTTAVFKIDIHCEGCANKLRRSVRQIPG---------------------- 60

Query: 61  SIFLRVRADWEANKLTVTGKFDPAKLRENLANKTKKKIDIVSSDSKKEKES-KKPDDDKQ 120
               RVRADWEANKLTV GKFDPAKLR+ LA+K  KKIDIVSS+SKKEKES KK DD+K 
Sbjct: 61  --VSRVRADWEANKLTVIGKFDPAKLRDYLADKETKKIDIVSSESKKEKESTKKQDDEKP 120

Query: 121 DKKTEDKK-PKDKETPVTTATLKVELHCQGCIEKIYKVVSRTRGVEDMAIERQKDLVMVK 180
           DKKTEDKK PKDKE PVTTATLKVELHCQGCIEKIYKVVSRT+GVEDMAIERQKDLVMVK
Sbjct: 121 DKKTEDKKQPKDKEIPVTTATLKVELHCQGCIEKIYKVVSRTKGVEDMAIERQKDLVMVK 180

Query: 181 GKMDVKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNKGGGEVAQEGGVMEGNRL 240
           GKMDVKALIENLEEKLKRKVAVVVPKKDKD+GAKGGDGGDKNK GGEVAQ GG MEGNRL
Sbjct: 181 GKMDVKALIENLEEKLKRKVAVVVPKKDKDEGAKGGDGGDKNKTGGEVAQGGGAMEGNRL 240

Query: 241 DYVAVPVPGYGYGYGYGYGYGYGNGGFVGQPLPSPQHLLSPQMFSDENPNACSVM 294
           DYVAVPVP  GYGYGYGYGYGYGNGGFVGQ +P  Q L+SPQMFSDENPNACSVM
Sbjct: 241 DYVAVPVP--GYGYGYGYGYGYGNGGFVGQHMPPAQPLISPQMFSDENPNACSVM 267

BLAST of Lsi10G012370 vs. ExPASy TrEMBL
Match: A0A5D3DG67 (Heavy metal-associated isoprenylated plant protein 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold173G00030 PE=4 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 2.7e-102
Identity = 210/231 (90.91%), Postives = 216/231 (93.51%), Query Frame = 0

Query: 65  RVRADWEANKLTVTGKFDPAKLRENLANKTKKKIDIVSSDSKKEKES-KKPDDDKQDKKT 124
           RVRADWEANKLTV GKFD AKLR+NLA+KTKKKIDIVSS+ KKEKES KK DD+K DKKT
Sbjct: 26  RVRADWEANKLTVIGKFDAAKLRDNLADKTKKKIDIVSSEGKKEKESAKKQDDEKPDKKT 85

Query: 125 EDKK-PKDKETPVTTATLKVELHCQGCIEKIYKVVSRTRGVEDMAIERQKDLVMVKGKMD 184
           EDKK PKDKE PVTTATLKVELHCQGCIEKIYKVVSRT+GVEDMAIERQKDLVMVKGKMD
Sbjct: 86  EDKKQPKDKEIPVTTATLKVELHCQGCIEKIYKVVSRTKGVEDMAIERQKDLVMVKGKMD 145

Query: 185 VKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNKGGGEVAQEGGVMEGNRLDYVA 244
           VKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNK GGEVAQEGG MEGNRLDYVA
Sbjct: 146 VKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNKTGGEVAQEGGAMEGNRLDYVA 205

Query: 245 VPVPGYGYGYGYGYGYGYGNGGFVGQPLPSPQHLLSPQMFSDENPNACSVM 294
           VPVP  GYGYGYGYGYGYGNGGFVGQ LP PQ LLSPQMFSDENPNACS+M
Sbjct: 206 VPVP--GYGYGYGYGYGYGNGGFVGQHLPQPQPLLSPQMFSDENPNACSLM 254

BLAST of Lsi10G012370 vs. ExPASy TrEMBL
Match: A0A6J1FUL5 (heavy metal-associated isoprenylated plant protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111448569 PE=4 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 1.2e-94
Identity = 202/301 (67.11%), Postives = 229/301 (76.08%), Query Frame = 0

Query: 1   MGEKKN------TEQDSTTAVFKIDIHCEGCANKIRKSVREIPGSYPFPIRYPIMIPNLT 60
           MG+K+N       E+DSTTAVF ID+HCEGCANK+R+ VR+I G                
Sbjct: 1   MGKKQNGSHGSDKEEDSTTAVFNIDMHCEGCANKLRRCVRQIQG---------------- 60

Query: 61  ITFPCSSIFLRVRADWEANKLTVTGKFDPAKLRENLANKTKKKIDIVSSDSKKEKESKKP 120
                     RVRADWEANKLTV GKFD + LRE LA+KT KKIDIVSS+ KKEK+SKKP
Sbjct: 61  --------VERVRADWEANKLTVIGKFDASTLREKLADKTNKKIDIVSSEGKKEKKSKKP 120

Query: 121 DDDKQDKKTEDKKPKDKETPVTTATLKVELHCQGCIEKIYKVVSRTRGVEDMAIERQKDL 180
           D++      ED+KPKDKE PVTTATLKVELHCQGC+E+IYKVVSRT+GVEDMA+ERQKDL
Sbjct: 121 DEE-----AEDEKPKDKEIPVTTATLKVELHCQGCVERIYKVVSRTKGVEDMAMERQKDL 180

Query: 181 VMVKGKMDVKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNKGGGEVAQE--GGV 240
           V VKGKMDVKAL+ NLE+KLKRKVAVVVPKKD DD    GDGGDKNK GGEV QE  GG 
Sbjct: 181 VTVKGKMDVKALVHNLEDKLKRKVAVVVPKKDNDD----GDGGDKNKSGGEVTQEDGGGE 240

Query: 241 MEGNRLDYVAVPVPGYGYGYGYGYGYGYGNGGFVGQPLPSPQHLLSPQMFSDENPNACSV 294
            +G+RLDY+AVP PGY YG+GYGYGYGYG+GGFVG      +HL S QMFSDENPNAC+V
Sbjct: 241 TDGDRLDYMAVPAPGYEYGFGYGYGYGYGHGGFVG------EHLPSSQMFSDENPNACTV 262

BLAST of Lsi10G012370 vs. ExPASy TrEMBL
Match: A0A6J1CGH5 (heavy metal-associated isoprenylated plant protein 3-like OS=Momordica charantia OX=3673 GN=LOC111011319 PE=4 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 2.7e-94
Identity = 206/302 (68.21%), Postives = 228/302 (75.50%), Query Frame = 0

Query: 1   MGEKKN-----TEQDSTTAVFKIDIHCEGCANKIRKSVREIPGSYPFPIRYPIMIPNLTI 60
           MG KKN      ++DS++ VFKIDIHCEGCANKIRK VREI G                 
Sbjct: 1   MGAKKNGDDGKKKEDSSSVVFKIDIHCEGCANKIRKCVREIEG----------------- 60

Query: 61  TFPCSSIFLRVRADWEANKLTVTGKFDPAKLRENLANKTKKKIDIVSSDSKKEKESKKPD 120
                    +V+A+WE NKLTV GKFD +KLRE LA+KTKKKIDIVSS S+K+KE    D
Sbjct: 61  -------VEKVKAEWEINKLTVIGKFDASKLREKLADKTKKKIDIVSSSSEKKKEK---D 120

Query: 121 DDKQDKKTEDKKPKDKETPVTTATLKVELHCQGCIEKIYKVVSRTRGVEDMAIERQKDLV 180
           +  +DKK E+KKPK+KETPVTTATLKVELHCQGCI+KIYKVVSRT+GVEDMAIERQKDLV
Sbjct: 121 EKAEDKKAEEKKPKEKETPVTTATLKVELHCQGCIQKIYKVVSRTKGVEDMAIERQKDLV 180

Query: 181 MVKGKMDVKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNKGGGEVAQE----GG 240
           MVKGKMDVKALI+NLEEKLKRKVAVVVPKKDKDDGAKGGD  D NK  G+ AQ+    GG
Sbjct: 181 MVKGKMDVKALIQNLEEKLKRKVAVVVPKKDKDDGAKGGDAADNNKTAGDAAQQPTTGGG 240

Query: 241 VMEGNRLDYVAVPVPGYGYGYGYGYGYGYGNGGFVGQPLPSPQHLLSPQMFSDENPNACS 294
           + EGNR DY AVPV     GYGYGYGYGYG GG+VG      QHL SPQMFSDENPNACS
Sbjct: 241 ITEGNRPDYAAVPV----QGYGYGYGYGYGPGGYVG------QHLPSPQMFSDENPNACS 265

BLAST of Lsi10G012370 vs. NCBI nr
Match: XP_038900689.1 (heavy metal-associated isoprenylated plant protein 3-like [Benincasa hispida])

HSP 1 Score: 463.0 bits (1190), Expect = 1.9e-126
Identity = 249/293 (84.98%), Postives = 258/293 (88.05%), Query Frame = 0

Query: 1   MGEKKNTEQDSTTAVFKIDIHCEGCANKIRKSVREIPGSYPFPIRYPIMIPNLTITFPCS 60
           MGEKKN EQDSTTAVFKIDIHCEGCANKIRK VREIPG +                    
Sbjct: 1   MGEKKNAEQDSTTAVFKIDIHCEGCANKIRKCVREIPGVH-------------------- 60

Query: 61  SIFLRVRADWEANKLTVTGKFDPAKLRENLANKTKKKIDIVSSDSKKEKESKKPDDDKQD 120
               RVRADWEANKLTVTGKFDPA LRE LA+KTKKKIDIVSS+SKKEKESKKPDDDKQD
Sbjct: 61  ----RVRADWEANKLTVTGKFDPANLREELADKTKKKIDIVSSESKKEKESKKPDDDKQD 120

Query: 121 KKTEDKKPKDKETPVTTATLKVELHCQGCIEKIYKVVSRTRGVEDMAIERQKDLVMVKGK 180
           KKTEDKKPKDKETPVTTATLKVELHCQGCIEKIYKVVSRT+GVEDMAIERQKDLVMVKGK
Sbjct: 121 KKTEDKKPKDKETPVTTATLKVELHCQGCIEKIYKVVSRTKGVEDMAIERQKDLVMVKGK 180

Query: 181 MDVKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNKGGGEVAQEGGVMEGNRLDY 240
           MDVKALIENLEEKLKRKVAVVVPKK+KDDGAKGGD GDKNK GGEVAQ+GGV +GNRLDY
Sbjct: 181 MDVKALIENLEEKLKRKVAVVVPKKEKDDGAKGGDVGDKNKSGGEVAQDGGVTDGNRLDY 240

Query: 241 VAVPVPGYGYGYGYGYGYGYGNGGFVGQPLPSPQHLLSPQMFSDENPNACSVM 294
           VAVPVP  GYGYGYGYGYGYGNGGFVGQ LP+PQHLLSPQMFSDENPNACS+M
Sbjct: 241 VAVPVP--GYGYGYGYGYGYGNGGFVGQHLPAPQHLLSPQMFSDENPNACSLM 267

BLAST of Lsi10G012370 vs. NCBI nr
Match: XP_008465354.1 (PREDICTED: heavy metal-associated isoprenylated plant protein 3-like [Cucumis melo])

HSP 1 Score: 433.0 bits (1112), Expect = 2.1e-117
Identity = 243/295 (82.37%), Postives = 250/295 (84.75%), Query Frame = 0

Query: 1   MGEKKNTEQDSTTAVFKIDIHCEGCANKIRKSVREIPGSYPFPIRYPIMIPNLTITFPCS 60
           MGEKKN   D TTAVFKIDIHCEGCANK+RKSVREIPG                      
Sbjct: 1   MGEKKN--DDITTAVFKIDIHCEGCANKLRKSVREIPG---------------------- 60

Query: 61  SIFLRVRADWEANKLTVTGKFDPAKLRENLANKTKKKIDIVSSDSKKEKES-KKPDDDKQ 120
               RVRADWEANKLTV GKFD AKLR+NLA+KTKKKIDIVSS+ KKEKES KK DD+K 
Sbjct: 61  --VARVRADWEANKLTVIGKFDAAKLRDNLADKTKKKIDIVSSEGKKEKESAKKQDDEKP 120

Query: 121 DKKTEDKK-PKDKETPVTTATLKVELHCQGCIEKIYKVVSRTRGVEDMAIERQKDLVMVK 180
           DKKTEDKK PKDKE PVTTATLKVELHCQGCIEKIYKVVSRT+GVEDMAIERQKDLVMVK
Sbjct: 121 DKKTEDKKQPKDKEIPVTTATLKVELHCQGCIEKIYKVVSRTKGVEDMAIERQKDLVMVK 180

Query: 181 GKMDVKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNKGGGEVAQEGGVMEGNRL 240
           GKMDVKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNK GGEVAQEGG MEGNRL
Sbjct: 181 GKMDVKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNKTGGEVAQEGGAMEGNRL 240

Query: 241 DYVAVPVPGYGYGYGYGYGYGYGNGGFVGQPLPSPQHLLSPQMFSDENPNACSVM 294
           DYVAVPVP  GYGYGYGYGYGYGNGGFVGQ LP PQ LLSPQMFSDENPNACS+M
Sbjct: 241 DYVAVPVP--GYGYGYGYGYGYGNGGFVGQHLPQPQPLLSPQMFSDENPNACSLM 267

BLAST of Lsi10G012370 vs. NCBI nr
Match: XP_004145758.1 (heavy metal-associated isoprenylated plant protein 3 [Cucumis sativus] >KGN46869.1 hypothetical protein Csa_020999 [Cucumis sativus])

HSP 1 Score: 420.2 bits (1079), Expect = 1.4e-113
Identity = 236/295 (80.00%), Postives = 248/295 (84.07%), Query Frame = 0

Query: 1   MGEKKNTEQDSTTAVFKIDIHCEGCANKIRKSVREIPGSYPFPIRYPIMIPNLTITFPCS 60
           MGEKKN   D+TTAVFKIDIHCEGCANK+R+SVR+IPG                      
Sbjct: 1   MGEKKN--DDTTTAVFKIDIHCEGCANKLRRSVRQIPG---------------------- 60

Query: 61  SIFLRVRADWEANKLTVTGKFDPAKLRENLANKTKKKIDIVSSDSKKEKES-KKPDDDKQ 120
               RVRADWEANKLTV GKFDPAKLR+ LA+K  KKIDIVSS+SKKEKES KK DD+K 
Sbjct: 61  --VSRVRADWEANKLTVIGKFDPAKLRDYLADKETKKIDIVSSESKKEKESTKKQDDEKP 120

Query: 121 DKKTEDKK-PKDKETPVTTATLKVELHCQGCIEKIYKVVSRTRGVEDMAIERQKDLVMVK 180
           DKKTEDKK PKDKE PVTTATLKVELHCQGCIEKIYKVVSRT+GVEDMAIERQKDLVMVK
Sbjct: 121 DKKTEDKKQPKDKEIPVTTATLKVELHCQGCIEKIYKVVSRTKGVEDMAIERQKDLVMVK 180

Query: 181 GKMDVKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNKGGGEVAQEGGVMEGNRL 240
           GKMDVKALIENLEEKLKRKVAVVVPKKDKD+GAKGGDGGDKNK GGEVAQ GG MEGNRL
Sbjct: 181 GKMDVKALIENLEEKLKRKVAVVVPKKDKDEGAKGGDGGDKNKTGGEVAQGGGAMEGNRL 240

Query: 241 DYVAVPVPGYGYGYGYGYGYGYGNGGFVGQPLPSPQHLLSPQMFSDENPNACSVM 294
           DYVAVPVP  GYGYGYGYGYGYGNGGFVGQ +P  Q L+SPQMFSDENPNACSVM
Sbjct: 241 DYVAVPVP--GYGYGYGYGYGYGNGGFVGQHMPPAQPLISPQMFSDENPNACSVM 267

BLAST of Lsi10G012370 vs. NCBI nr
Match: KAA0035228.1 (heavy metal-associated isoprenylated plant protein 3-like [Cucumis melo var. makuwa] >TYK22380.1 heavy metal-associated isoprenylated plant protein 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 381.7 bits (979), Expect = 5.6e-102
Identity = 210/231 (90.91%), Postives = 216/231 (93.51%), Query Frame = 0

Query: 65  RVRADWEANKLTVTGKFDPAKLRENLANKTKKKIDIVSSDSKKEKES-KKPDDDKQDKKT 124
           RVRADWEANKLTV GKFD AKLR+NLA+KTKKKIDIVSS+ KKEKES KK DD+K DKKT
Sbjct: 26  RVRADWEANKLTVIGKFDAAKLRDNLADKTKKKIDIVSSEGKKEKESAKKQDDEKPDKKT 85

Query: 125 EDKK-PKDKETPVTTATLKVELHCQGCIEKIYKVVSRTRGVEDMAIERQKDLVMVKGKMD 184
           EDKK PKDKE PVTTATLKVELHCQGCIEKIYKVVSRT+GVEDMAIERQKDLVMVKGKMD
Sbjct: 86  EDKKQPKDKEIPVTTATLKVELHCQGCIEKIYKVVSRTKGVEDMAIERQKDLVMVKGKMD 145

Query: 185 VKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNKGGGEVAQEGGVMEGNRLDYVA 244
           VKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNK GGEVAQEGG MEGNRLDYVA
Sbjct: 146 VKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNKTGGEVAQEGGAMEGNRLDYVA 205

Query: 245 VPVPGYGYGYGYGYGYGYGNGGFVGQPLPSPQHLLSPQMFSDENPNACSVM 294
           VPVP  GYGYGYGYGYGYGNGGFVGQ LP PQ LLSPQMFSDENPNACS+M
Sbjct: 206 VPVP--GYGYGYGYGYGYGNGGFVGQHLPQPQPLLSPQMFSDENPNACSLM 254

BLAST of Lsi10G012370 vs. NCBI nr
Match: KAG7010652.1 (Heavy metal-associated isoprenylated plant protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 357.8 bits (917), Expect = 8.7e-95
Identity = 200/294 (68.03%), Postives = 226/294 (76.87%), Query Frame = 0

Query: 2   GEKKNTEQDSTTAVFKIDIHCEGCANKIRKSVREIPGSYPFPIRYPIMIPNLTITFPCSS 61
           G   + ++DSTTAVF ID+HCEGCANK+R+ VR+I G                       
Sbjct: 5   GHGSDKQEDSTTAVFNIDMHCEGCANKLRRCVRQIQG----------------------- 64

Query: 62  IFLRVRADWEANKLTVTGKFDPAKLRENLANKTKKKIDIVSSDSKKEKESKKPDDDKQDK 121
              RVR DWEANKLTV GKFD + LRE LA+KTKKKIDIVSS+ KKEK+SKKPD++    
Sbjct: 65  -VERVRVDWEANKLTVIGKFDASTLREKLADKTKKKIDIVSSEGKKEKKSKKPDEE---- 124

Query: 122 KTEDKKPKDKETPVTTATLKVELHCQGCIEKIYKVVSRTRGVEDMAIERQKDLVMVKGKM 181
             ED+KPKDKE PVTTATLKVELHCQGC+E+IYKVVSRT+GVEDMA+ERQKDLV VKGKM
Sbjct: 125 -AEDEKPKDKEIPVTTATLKVELHCQGCVERIYKVVSRTKGVEDMAMERQKDLVTVKGKM 184

Query: 182 DVKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNKGGGEVAQE--GGVMEGNRLD 241
           DVKAL+ NLE+KLKRKVAVVVPKKD DD    GDGGDKNK GGEVAQE  GG  +G+RLD
Sbjct: 185 DVKALVHNLEDKLKRKVAVVVPKKDSDD----GDGGDKNKSGGEVAQENGGGETDGDRLD 244

Query: 242 YVAVPVPGYGYGYGYGYGYGYGNGGFVGQPLPSPQHLLSPQMFSDENPNACSVM 294
           Y+AVP PGY YGYGYGYGYGYG+GGFVG      +HL S QMFSDENPNAC+VM
Sbjct: 245 YMAVPAPGYEYGYGYGYGYGYGHGGFVG------EHLPSSQMFSDENPNACTVM 259

BLAST of Lsi10G012370 vs. TAIR 10
Match: AT5G60800.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 202.6 bits (514), Expect = 4.4e-52
Identity = 137/304 (45.07%), Postives = 173/304 (56.91%), Query Frame = 0

Query: 4   KKNTEQDSTTAVFKIDIHCEGCANKIRKSVREIPGSYPFPIRYPIMIPNLTITFPCSSIF 63
           KK  E  S T V K+D+HCEGCA++I K VR   G                         
Sbjct: 18  KKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQG------------------------V 77

Query: 64  LRVRADWEANKLTVTGKFDPAKLRENLANKTKKKIDIVSSDSK--KEKESKKPDDDKQDK 123
             V+++    KLTVTG  DP KLRE L  KTKKK+D+VS   K  KEKE+K  +D+ + K
Sbjct: 78  ETVKSESATGKLTVTGALDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKK 137

Query: 124 KTEDKKP-----KDKETPVTTATLKVELHCQGCIEKIYKVVSRTRGVEDMAIERQKDLVM 183
             E KKP     K KETPVTTA LK+  HCQGCI KI K V++T+GV  + ++++K+L+ 
Sbjct: 138 SEEKKKPDNNDKKPKETPVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLT 197

Query: 184 VKGKMDVKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNKGGG-------EVAQE 243
           VKG MDVK L+E L EKLKR V +V PKK+KD    G + G+K KGGG       +   +
Sbjct: 198 VKGTMDVKKLVEILSEKLKRAVEIVPPKKEKDK-ENGNENGEKKKGGGGDGGGKEKTGNK 257

Query: 244 GGVMEGNRLDYVAVPVPGYGYGYGYGYGYGYGNGGFVGQPLPSPQHLLSPQMFSDENPNA 294
           GG    N ++Y+A   P YGYGY  G  YGY            P    +PQ+FSDENPNA
Sbjct: 258 GGGEGVNMMEYMAAQ-PAYGYGYYPGGPYGY------------PIQAHAPQIFSDENPNA 283

BLAST of Lsi10G012370 vs. TAIR 10
Match: AT5G60800.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 201.1 bits (510), Expect = 1.3e-51
Identity = 136/304 (44.74%), Postives = 173/304 (56.91%), Query Frame = 0

Query: 4   KKNTEQDSTTAVFKIDIHCEGCANKIRKSVREIPGSYPFPIRYPIMIPNLTITFPCSSIF 63
           KK  E  S T V K+D+HCEGCA++I K VR   G                         
Sbjct: 18  KKKNETPSITVVLKVDMHCEGCASRIVKCVRSFQG------------------------V 77

Query: 64  LRVRADWEANKLTVTGKFDPAKLRENLANKTKKKIDIVSSDSK--KEKESKKPDDDKQDK 123
             V+++    KLTVTG  DP KLRE L  KTKKK+D+VS   K  KEKE+K  +D+ + K
Sbjct: 78  ETVKSESATGKLTVTGALDPVKLREKLEEKTKKKVDLVSPQPKKEKEKENKNKNDEDKKK 137

Query: 124 KTEDKKP-----KDKETPVTTATLKVELHCQGCIEKIYKVVSRTRGVEDMAIERQKDLVM 183
             E KKP     K KETPVTTA LK+  HCQGCI KI K V++T+GV  + ++++K+L+ 
Sbjct: 138 SEEKKKPDNNDKKPKETPVTTAVLKLNFHCQGCIGKIQKTVTKTKGVNGLTMDKEKNLLT 197

Query: 184 VKGKMDVKALIENLEEKLKRKVAVVVPKKDKDDGAKGGDGGDKNKGGG-------EVAQE 243
           VKG MDVK L+E L EKLKR V +V PKK+KD    G + G+K KGGG       +   +
Sbjct: 198 VKGTMDVKKLVEILSEKLKRAVEIVPPKKEKDK-ENGNENGEKKKGGGGDGGGKEKTGNK 257

Query: 244 GGVMEGNRLDYVAVPVPGYGYGYGYGYGYGYGNGGFVGQPLPSPQHLLSPQMFSDENPNA 294
           GG    N ++Y+A   P YGYGY  G  YGY            P    +PQ+FSDENPNA
Sbjct: 258 GGGEGVNMMEYMAAQ-PAYGYGYYPGGPYGY------------PIQAHAPQIFSDENPNA 283

BLAST of Lsi10G012370 vs. TAIR 10
Match: AT5G03380.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 136.3 bits (342), Expect = 3.8e-32
Identity = 125/403 (31.02%), Postives = 176/403 (43.67%), Query Frame = 0

Query: 2   GEKKNTEQDSTTAVFKIDIHCEGCANKIRKSVREIPGSYPFPIRYPIMIPNLTITFPCSS 61
           GEKK T+   TT V K+D+HCEGC  KI++  +   G                       
Sbjct: 14  GEKKPTDGGITTVVMKLDMHCEGCGKKIKRIFKHFKGVED-------------------- 73

Query: 62  IFLRVRADWEANKLTVTGKFDPAKLRENLANKTKKKIDIVSSDSKKEKES---------- 121
               V+ D+++NKLTV G  DP ++R+ +A+K K+ +++VS+ +  +KE+          
Sbjct: 74  ----VKIDYKSNKLTVIGNVDPVEVRDKVADKIKRPVELVSTVAPPKKETPPSSGGAEKK 133

Query: 122 -----------------KKPDDDKQDKKTEDKKPKDKETP---VTTATLKVELHCQGCIE 181
                            +KP + K++KK E+ + K    P    +T  LK +LHC+GC  
Sbjct: 134 PSPAAEEKPAEKKPAAVEKPGEKKEEKKKEEGEKKASPPPPPKESTVVLKTKLHCEGCEH 193

Query: 182 KIYKVVSRTRGVEDMAIERQKDLVMVKGKMDVKALIENLEEKLKRKVAVVVPKKD----- 241
           KI ++V++ +GV  +AI+  KDLV+VKG +DVK L   L EKLKR V VV  KKD     
Sbjct: 194 KIKRIVNKIKGVNSVAIDSAKDLVIVKGIIDVKQLTPYLNEKLKRTVEVVPAKKDDGAPV 253

Query: 242 -----------------------KDDGAKGGDGGDKNK---------GGGEVAQEGGVME 294
                                  KD G K  DGG + K         GGG    +GG M+
Sbjct: 254 AAAAAAPAGGEKKDKVAGEKKEIKDVGEKKVDGGGEKKKEVAVGGGGGGGGGGGDGGAMD 313

BLAST of Lsi10G012370 vs. TAIR 10
Match: AT2G36950.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 127.5 bits (319), Expect = 1.8e-29
Identity = 120/362 (33.15%), Postives = 161/362 (44.48%), Query Frame = 0

Query: 15  VFKIDIHCEGCANKIRKSVREIPGSYPFPIRYPIMIPNLTITFPCSSIFLRVRADWEANK 74
           V+K+D+HCEGCA KI++ V+   G                           V AD   NK
Sbjct: 53  VYKVDMHCEGCAKKIKRMVKHFDG------------------------VKDVTADTGGNK 112

Query: 75  LTVTGKFDPAKLRENLANKTKKKIDIVSSDSKKEKESKKPDDDKQ----DKKTEDKKPKD 134
           L V GK DP KL+E L  KTK+K+ + +   K E        +K+    DK+     P  
Sbjct: 113 LLVVGKIDPVKLQEKLEEKTKRKVVLANPPPKVEGPVAAAVGEKKADGGDKEAAPPAPAP 172

Query: 135 KETPVTTATLKVELHCQGCIEKIYKVVSRTRGVEDMAIERQKDLVMVKGKMDVKALIENL 194
                +   LK+ LHC+GCI+KI K++ + +GVE +AI+  KD+V VKG +DVK L+  L
Sbjct: 173 AAPKESVVPLKIRLHCEGCIQKIKKIILKIKGVETVAIDGAKDVVTVKGTIDVKELVPLL 232

Query: 195 EEKLKRKVAVVVPKKDKDDGA--------------------------------------- 254
            +KLKR V  +VP K KDDGA                                       
Sbjct: 233 TKKLKRTVEPLVPAK-KDDGAAENKKTEAAAPDAKKEAPSAGVNEAKKEGSDGGEKKKEV 292

Query: 255 --------KGGDGGDKNK---GGGEVAQEGG-----VMEGNRLDYV---AVPV------P 294
                   +GGDGG+K K    GGE  ++GG     V   N++DY    A P        
Sbjct: 293 GDGGEKKKEGGDGGEKKKEAGDGGEKKKDGGGVPAPVAMVNKMDYYGYSAYPTAPMHWQE 352

BLAST of Lsi10G012370 vs. TAIR 10
Match: AT5G03380.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 122.9 bits (307), Expect = 4.4e-28
Identity = 117/388 (30.15%), Postives = 167/388 (43.04%), Query Frame = 0

Query: 17  KIDIHCEGCANKIRKSVREIPGSYPFPIRYPIMIPNLTITFPCSSIFLRVRADWEANKLT 76
           K+D+HCEGC  KI++  +   G                           V+ D+++NKLT
Sbjct: 2   KLDMHCEGCGKKIKRIFKHFKGVED------------------------VKIDYKSNKLT 61

Query: 77  VTGKFDPAKLRENLANKTKKKIDIVSSDSKKEKES------------------------- 136
           V G  DP ++R+ +A+K K+ +++VS+ +  +KE+                         
Sbjct: 62  VIGNVDPVEVRDKVADKIKRPVELVSTVAPPKKETPPSSGGAEKKPSPAAEEKPAEKKPA 121

Query: 137 --KKPDDDKQDKKTEDKKPKDKETP---VTTATLKVELHCQGCIEKIYKVVSRTRGVEDM 196
             +KP + K++KK E+ + K    P    +T  LK +LHC+GC  KI ++V++ +GV  +
Sbjct: 122 AVEKPGEKKEEKKKEEGEKKASPPPPPKESTVVLKTKLHCEGCEHKIKRIVNKIKGVNSV 181

Query: 197 AIERQKDLVMVKGKMDVKALIENLEEKLKRKVAVVVPKKD-------------------- 256
           AI+  KDLV+VKG +DVK L   L EKLKR V VV  KKD                    
Sbjct: 182 AIDSAKDLVIVKGIIDVKQLTPYLNEKLKRTVEVVPAKKDDGAPVAAAAAAPAGGEKKDK 241

Query: 257 --------KDDGAKGGDGGDKNK---------GGGEVAQEGGVMEGNRLDYVAV---PVP 294
                   KD G K  DGG + K         GGG    +GG M+  + +Y      P P
Sbjct: 242 VAGEKKEIKDVGEKKVDGGGEKKKEVAVGGGGGGGGGGGDGGAMDVKKSEYNGYGYPPQP 301

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FJH56.1e-5145.07Heavy metal-associated isoprenylated plant protein 3 OS=Arabidopsis thaliana OX=... [more]
Q9LZF15.4e-3131.02Heavy metal-associated isoprenylated plant protein 6 OS=Arabidopsis thaliana OX=... [more]
Q9SJL22.5e-2833.15Heavy metal-associated isoprenylated plant protein 5 OS=Arabidopsis thaliana OX=... [more]
Q9ZUV11.5e-2032.43Heavy metal-associated isoprenylated plant protein 1 OS=Arabidopsis thaliana OX=... [more]
Q9C5D31.9e-1528.21Heavy metal-associated isoprenylated plant protein 7 OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
A0A1S3CP341.0e-11782.37heavy metal-associated isoprenylated plant protein 3-like OS=Cucumis melo OX=365... [more]
A0A0A0KDD76.9e-11480.00HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G147620 PE=4 SV... [more]
A0A5D3DG672.7e-10290.91Heavy metal-associated isoprenylated plant protein 3-like OS=Cucumis melo var. m... [more]
A0A6J1FUL51.2e-9467.11heavy metal-associated isoprenylated plant protein 3 OS=Cucurbita moschata OX=36... [more]
A0A6J1CGH52.7e-9468.21heavy metal-associated isoprenylated plant protein 3-like OS=Momordica charantia... [more]
Match NameE-valueIdentityDescription
XP_038900689.11.9e-12684.98heavy metal-associated isoprenylated plant protein 3-like [Benincasa hispida][more]
XP_008465354.12.1e-11782.37PREDICTED: heavy metal-associated isoprenylated plant protein 3-like [Cucumis me... [more]
XP_004145758.11.4e-11380.00heavy metal-associated isoprenylated plant protein 3 [Cucumis sativus] >KGN46869... [more]
KAA0035228.15.6e-10290.91heavy metal-associated isoprenylated plant protein 3-like [Cucumis melo var. mak... [more]
KAG7010652.18.7e-9568.03Heavy metal-associated isoprenylated plant protein 3, partial [Cucurbita argyros... [more]
Match NameE-valueIdentityDescription
AT5G60800.14.4e-5245.07Heavy metal transport/detoxification superfamily protein [more]
AT5G60800.21.3e-5144.74Heavy metal transport/detoxification superfamily protein [more]
AT5G03380.13.8e-3231.02Heavy metal transport/detoxification superfamily protein [more]
AT2G36950.11.8e-2933.15Heavy metal transport/detoxification superfamily protein [more]
AT5G03380.24.4e-2830.15Heavy metal transport/detoxification superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006121Heavy metal-associated domain, HMAPFAMPF00403HMAcoord: 139..193
e-value: 3.6E-8
score: 33.7
IPR006121Heavy metal-associated domain, HMACDDcd00371HMAcoord: 139..192
e-value: 0.0012015
score: 34.5037
NoneNo IPR availableGENE3D3.30.70.100coord: 135..208
e-value: 7.9E-14
score: 53.5
NoneNo IPR availableGENE3D3.30.70.100coord: 7..98
e-value: 7.1E-13
score: 50.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 205..219
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 97..134
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 205..229
NoneNo IPR availablePANTHERPTHR46413:SF2HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 3coord: 5..38
NoneNo IPR availablePANTHERPTHR46413:SF2HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 3coord: 66..293
IPR044594Heavy metal-associated isoprenylated plant protein 1/3/5/6PANTHERPTHR46413HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 6coord: 66..293
coord: 5..38
IPR036163Heavy metal-associated domain superfamilySUPERFAMILY55008HMA, heavy metal-associated domaincoord: 135..192
IPR036163Heavy metal-associated domain superfamilySUPERFAMILY55008HMA, heavy metal-associated domaincoord: 9..40

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G012370.1Lsi10G012370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding