Lsi10G012050 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G012050
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPhospholipid:diacylglycerol acyltransferase
Locationchr10: 16338364 .. 16342105 (+)
RNA-Seq ExpressionLsi10G012050
SyntenyLsi10G012050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTCTGTTCTTCGTCTCCGCAAATTATACTTCGTCAACCCTGTTAAGAGCTCTTCTATGGGGGTTCAACCCATTGAAGCACATAAATCAGAGGATAAACATAATGTCATTGTCGCATATGTGAAGAAATTGATGCAAATGAAGCAAGACAAGGACAAGAAGAAGAAAAGGAACAAGAACATCAAGAAGGGGCAATCAGGAGAATGGAAATGCTTAGATAACTGCTGTTGGATTATTGGATACATGTGTACATTTTGGTGGCTGATGTTTTTTCTCTACCATTATTTGCCTCTTACACTACCTGGCTTTCGGGCGATTGATTCTCCGGGAGCTGTTCTAAAGCGAGAAGGCATATCAGGTCTTCACCCAGTCGTTTTAGTGCCTGGAATTGTCACCGGCGGGCTGGAGCTCTGGGACGGCAAGCCCTGCGCCGAGGGCCTCTTTCGAAAGCGCCTGTGGGGTGGGAGTTTCACTGAAACATTGAAAAGGTTAGCAAATTCCAGATCAATTTGACATTTCTGAAGCTTTTGAATTGATTTTGGAAGTACCCATTTGAAAATTTGAATTCAAATTTTCAGACCACTATGCTGGTTGGAGCACCTGTCTCTAGACAATGAAACAGGGCTTGACCCACCAGGCATTCGGGTCCGGCCAGTTGAAGGCCTTGTGGCTGCTGATTATTTCGCCCAAGGCTACTTCGTTTGGGCTGTTCTCATTGAAAATCTGGCTAAAATTGGTTATGATGGAAAGAACTTGCACATGGCTGCATATGATTGGAGGATCGCCTTCCAAAATACAGAGGTATGTGACCCGAAACAGTCTTCTTCTTGCAATCATATATATATATCATCTCTTCATTGGGTTTGCCATAGTTGAACTGAGAAATCACGCAGGTTAGAGACAGAGCCCTGAGCAGATTGAAGAGTAAAATAGAGGTAATGTATGAAACAAATGGGGGGAATAAAGTGGTGGTGGTGCCTCATTCAATGGGGGTTCTGTATTTCCTGCACTTCATGAAGTGGGTGGAAGCGCCGCCGCCGATGGGCGGCGGCGGCGGCGGCCTTGGGTGGTGTGCCAAGCATATTAAAGCAATCATGAATATTGGCTCTACATTCTTGGGAACTCCAAAGGCAGTCAGTAAAATTCTTTCTGCTGAAGACAGACATGTTGCTTTGCTCAGGTTCACTTCGATGTTCTCTTCTTTTCTTCACTCTAAATATTGTAAGTTTGTAATGTAACTATTATCTTTAAAGTATTTTATTTGTATTTAATGAATAAATTTTGTCGTTATTGTAATTTGTTATCAGTTTCCATTTCTCATTCTTATTTTCTTTTTTCACAGGTGTTTTGACAAAGTCTTTTAATCTTCGTTTCTCATTTTTCATATTCAATAGCAAATGGTTTAAAGTATTTTGGATTAAATGTTTTGTTTATAGAGTTTCAGAATTTATTGATAAACTTCATTTCAAATTTCACTAACTTTTCTGAACTAGAATTTTCAAGATAGAATTTATTTTTGAAATAAATGAGATGGATTTTTTTTTTTTTTTGGTATTTATTTCTCAAGAGGAAAAGAACTAAGAAATACAATACTTTTATAATTGTGTCAAAATTTTGACTGGAAGTGTAGGATTTCTCAGGCTTTGTGAATTATTAGAGAAAGGCATTTAGAATTTAGCCATCAAATATTTATTGAAGACAGAGAAAAAATTCTTAAGCAATGAAGAAGAGATTAACCTACAAATGACAGACAGAGAGAAGTCTTCTTATAGAGATTCAAGGACAAATAATGAACTTGGTTTTCTTGGCATTTTACAGAGCTATGACCCCTGGTTTTCTTAAATCTGAAATTCTTGGCTTTCAAACCTTAGAACATTTGTTGCGGGTTTCCCGAACGTGGGATTCGACCGCCTCCTTGTTGCCAAAAGGTGGTGAGACCATCTGGGGTGACTTAGATTGGTCTCCTGAAGATGTTCAGAATTGTGATTTGAAGAAAGTAGATACACATTCTTTTAGAAACGAAAATGATTCCAATGACAGTAAAAGAAAGAAAGCTTTCCAAGAAAAGGAGCCAGTGCACTATGGAAGAATAATCTCTTTTAGCAAAGAAGCAGCAACACGACCTTCTTCAGAGCTCTCTACACTAAATTCAGAGGTAATTTCTCGGCTACTTTCTGTGCATTGGAATTAAATTCTTTGATGGGGGCAAATGATGAAACTTAGCAGCGCCGAATGTGTTAGATACTACTTTTTTTTTTTTTTTTTTTTGATAGGTGAGCTTAGATATTCAAAGTGGGACATTTTTCTCTATATCTTGAGCGTAGGATAGTATTCACTAATGAGTGGATCTTGAATAGGAACTTAAAAATTCAGGTAGCTCCAATGATTCGAGCTTCTCTTGTAGCGATGTATGGACTGAATATGATGAGATGAATAGGGAAAGCATTCGAAAAGTCTCTGTGAATAAAGCCTACACAGTGAAAACTGTTTTCGATCTACTCCGAGTTGTGGCTCCAAAAATGATGCAGCGAATGGATAGTCACTTCTCTCATGGGATTGCTGAAGATCTTGATGATCCTAAATATGCTCATTATAAGTACTGGTCAAATCCACTTGAGACAAAGTAAGTACCTGCGTAGATTTTTAGAATTTTCTGATTTTCTTTAGAAGATAATCTTCCACAATGAAGTACTGTCAACTCTCGGCCAGCTGATAATCCAATAACTATCTAACATCAAGAACATGGTTTTGGAGGTATTATTTGCTAATTTCAAATGCAATCAAACAACTTCCCTCAATCACTGTCCCCAAAAACAATACACACATTTCTCAACTTTCCGTTTCATTACTTCTGTCTTGCGGCCAGTCCTGAAACTAAATACATTGTTGAAAATCCGTTTCAGACTACCAGATGCCCCAGATATGGAGATATACTGCTTATATGGAGTGGGAATCCCCACAGAAAGATCATATATCTATGACTCATCTTATGATAAGTGCAAGAGCATTCCATTCAGGATTGATCTCTCAATTGAGGGAAAAGGGAGTGGCTGTCTGAGAAGCGGTATGTACTTTGTCGACGGTGACGACTCTGTACCGGTAGTGAGCTCCGGCTTCATGTGTGCAAAAGGGTGGCGAAACAAAACACGATTCAACCCATCAGGTAGCCCTACATACGTAAGAGAGTACAGGCACAAGGCTCCTGCAAGTCTCCTAGAAGGAAGAGGAGTTGAAAGTAGTTCTCACGTTGATATAATGGGAAATGTTAATCTTATTGAAGATATCTTGAGGGTTGCGGCTGGAGCCACTGGAGCAGAGATTGGTGGTGACAAGATTTACTCAGATATATTAACACTATCAGAGAGAATAAATCTTAAGCTGTGATTCGGTTTGAAAGCTCAAGAGATGGAGTTGAAAGGAAAATTAACGGGCTTCTAGAGCAGGATAGCATAAAATGGAGGGTATAGAAGAGAGGAAAATAATTCATACTCTTGGCAATGGAGTTTGTTCCCCCGGGTTTTTTCATGTGAATAATATTGTGATGTTTGAGGTCTACTTTCTAAACCCGATCATTGTCCTTCCAACATGTCCATTTTCGGTGCCTCTACTTTCTAAAATATCATACAATCTTCTGAAACATAACTTGCCACAGTGTAACCTAGGAGTTACGAATATGATATTGTCTATTTGTCAAATTCAGACTAAAAAATGTAGGTGGGTTTTGCGATTTGGTAGCAGG

mRNA sequence

ATGGCCTCTGTTCTTCGTCTCCGCAAATTATACTTCGTCAACCCTGTTAAGAGCTCTTCTATGGGGGTTCAACCCATTGAAGCACATAAATCAGAGGATAAACATAATGTCATTGTCGCATATGTGAAGAAATTGATGCAAATGAAGCAAGACAAGGACAAGAAGAAGAAAAGGAACAAGAACATCAAGAAGGGGCAATCAGGAGAATGGAAATGCTTAGATAACTGCTGTTGGATTATTGGATACATGTGTACATTTTGGTGGCTGATGTTTTTTCTCTACCATTATTTGCCTCTTACACTACCTGGCTTTCGGGCGATTGATTCTCCGGGAGCTGTTCTAAAGCGAGAAGGCATATCAGGTCTTCACCCAGTCGTTTTAGTGCCTGGAATTGTCACCGGCGGGCTGGAGCTCTGGGACGGCAAGCCCTGCGCCGAGGGCCTCTTTCGAAAGCGCCTGTGGGGTGGGAGTTTCACTGAAACATTGAAAAGACCACTATGCTGGTTGGAGCACCTGTCTCTAGACAATGAAACAGGGCTTGACCCACCAGGCATTCGGGTCCGGCCAGTTGAAGGCCTTGTGGCTGCTGATTATTTCGCCCAAGGCTACTTCGTTTGGGCTGTTCTCATTGAAAATCTGGCTAAAATTGGTTATGATGGAAAGAACTTGCACATGGCTGCATATGATTGGAGGATCGCCTTCCAAAATACAGAGGTTAGAGACAGAGCCCTGAGCAGATTGAAGAGTAAAATAGAGGTAATGTATGAAACAAATGGGGGGAATAAAGTGGTGGTGGTGCCTCATTCAATGGGGGTTCTGTATTTCCTGCACTTCATGAAGTGGGTGGAAGCGCCGCCGCCGATGGGCGGCGGCGGCGGCGGCCTTGGGTGGTGTGCCAAGCATATTAAAGCAATCATGAATATTGGCTCTACATTCTTGGGAACTCCAAAGGCAGTCAGTAAAATTCTTTCTGCTGAAGACAGACATGTTGCTTTGCTCAGAGCTATGACCCCTGGTTTTCTTAAATCTGAAATTCTTGGCTTTCAAACCTTAGAACATTTGTTGCGGGTTTCCCGAACGTGGGATTCGACCGCCTCCTTGTTGCCAAAAGGTGGTGAGACCATCTGGGGTGACTTAGATTGGTCTCCTGAAGATGTTCAGAATTGTGATTTGAAGAAAGTAGATACACATTCTTTTAGAAACGAAAATGATTCCAATGACAGTAAAAGAAAGAAAGCTTTCCAAGAAAAGGAGCCAGTGCACTATGGAAGAATAATCTCTTTTAGCAAAGAAGCAGCAACACGACCTTCTTCAGAGCTCTCTACACTAAATTCAGAGGAACTTAAAAATTCAGGTAGCTCCAATGATTCGAGCTTCTCTTGTAGCGATGTATGGACTGAATATGATGAGATGAATAGGGAAAGCATTCGAAAAGTCTCTGTGAATAAAGCCTACACAGTGAAAACTGTTTTCGATCTACTCCGAGTTGTGGCTCCAAAAATGATGCAGCGAATGGATAGTCACTTCTCTCATGGGATTGCTGAAGATCTTGATGATCCTAAATATGCTCATTATAAGTACTGGTCAAATCCACTTGAGACAAAACTACCAGATGCCCCAGATATGGAGATATACTGCTTATATGGAGTGGGAATCCCCACAGAAAGATCATATATCTATGACTCATCTTATGATAAGTGCAAGAGCATTCCATTCAGGATTGATCTCTCAATTGAGGGAAAAGGGAGTGGCTGTCTGAGAAGCGGTATGTACTTTGTCGACGGTGACGACTCTGTACCGGTAGTGAGCTCCGGCTTCATGTGTGCAAAAGGGTGGCGAAACAAAACACGATTCAACCCATCAGGTAGCCCTACATACGTAAGAGAGTACAGGCACAAGGCTCCTGCAAGTCTCCTAGAAGGAAGAGGAGTTGAAAGTAGTTCTCACGTTGATATAATGGGAAATGTTAATCTTATTGAAGATATCTTGAGGGTTGCGGCTGGAGCCACTGGAGCAGAGATTGGTGGTGACAAGATTTACTCAGATATATTAACACTATCAGAGAGAATAAATCTTAAGCTGTGATTCGGTTTGAAAGCTCAAGAGATGGAGTTGAAAGGAAAATTAACGGGCTTCTAGAGCAGGATAGCATAAAATGGAGGGTATAGAAGAGAGGAAAATAATTCATACTCTTGGCAATGGAGTTTGTTCCCCCGGGTTTTTTCATGTGAATAATATTGTGATGTTTGAGGTCTACTTTCTAAACCCGATCATTGTCCTTCCAACATGTCCATTTTCGGTGCCTCTACTTTCTAAAATATCATACAATCTTCTGAAACATAACTTGCCACAGTGTAACCTAGGAGTTACGAATATGATATTGTCTATTTGTCAAATTCAGACTAAAAAATGTAGGTGGGTTTTGCGATTTGGTAGCAGG

Coding sequence (CDS)

ATGGCCTCTGTTCTTCGTCTCCGCAAATTATACTTCGTCAACCCTGTTAAGAGCTCTTCTATGGGGGTTCAACCCATTGAAGCACATAAATCAGAGGATAAACATAATGTCATTGTCGCATATGTGAAGAAATTGATGCAAATGAAGCAAGACAAGGACAAGAAGAAGAAAAGGAACAAGAACATCAAGAAGGGGCAATCAGGAGAATGGAAATGCTTAGATAACTGCTGTTGGATTATTGGATACATGTGTACATTTTGGTGGCTGATGTTTTTTCTCTACCATTATTTGCCTCTTACACTACCTGGCTTTCGGGCGATTGATTCTCCGGGAGCTGTTCTAAAGCGAGAAGGCATATCAGGTCTTCACCCAGTCGTTTTAGTGCCTGGAATTGTCACCGGCGGGCTGGAGCTCTGGGACGGCAAGCCCTGCGCCGAGGGCCTCTTTCGAAAGCGCCTGTGGGGTGGGAGTTTCACTGAAACATTGAAAAGACCACTATGCTGGTTGGAGCACCTGTCTCTAGACAATGAAACAGGGCTTGACCCACCAGGCATTCGGGTCCGGCCAGTTGAAGGCCTTGTGGCTGCTGATTATTTCGCCCAAGGCTACTTCGTTTGGGCTGTTCTCATTGAAAATCTGGCTAAAATTGGTTATGATGGAAAGAACTTGCACATGGCTGCATATGATTGGAGGATCGCCTTCCAAAATACAGAGGTTAGAGACAGAGCCCTGAGCAGATTGAAGAGTAAAATAGAGGTAATGTATGAAACAAATGGGGGGAATAAAGTGGTGGTGGTGCCTCATTCAATGGGGGTTCTGTATTTCCTGCACTTCATGAAGTGGGTGGAAGCGCCGCCGCCGATGGGCGGCGGCGGCGGCGGCCTTGGGTGGTGTGCCAAGCATATTAAAGCAATCATGAATATTGGCTCTACATTCTTGGGAACTCCAAAGGCAGTCAGTAAAATTCTTTCTGCTGAAGACAGACATGTTGCTTTGCTCAGAGCTATGACCCCTGGTTTTCTTAAATCTGAAATTCTTGGCTTTCAAACCTTAGAACATTTGTTGCGGGTTTCCCGAACGTGGGATTCGACCGCCTCCTTGTTGCCAAAAGGTGGTGAGACCATCTGGGGTGACTTAGATTGGTCTCCTGAAGATGTTCAGAATTGTGATTTGAAGAAAGTAGATACACATTCTTTTAGAAACGAAAATGATTCCAATGACAGTAAAAGAAAGAAAGCTTTCCAAGAAAAGGAGCCAGTGCACTATGGAAGAATAATCTCTTTTAGCAAAGAAGCAGCAACACGACCTTCTTCAGAGCTCTCTACACTAAATTCAGAGGAACTTAAAAATTCAGGTAGCTCCAATGATTCGAGCTTCTCTTGTAGCGATGTATGGACTGAATATGATGAGATGAATAGGGAAAGCATTCGAAAAGTCTCTGTGAATAAAGCCTACACAGTGAAAACTGTTTTCGATCTACTCCGAGTTGTGGCTCCAAAAATGATGCAGCGAATGGATAGTCACTTCTCTCATGGGATTGCTGAAGATCTTGATGATCCTAAATATGCTCATTATAAGTACTGGTCAAATCCACTTGAGACAAAACTACCAGATGCCCCAGATATGGAGATATACTGCTTATATGGAGTGGGAATCCCCACAGAAAGATCATATATCTATGACTCATCTTATGATAAGTGCAAGAGCATTCCATTCAGGATTGATCTCTCAATTGAGGGAAAAGGGAGTGGCTGTCTGAGAAGCGGTATGTACTTTGTCGACGGTGACGACTCTGTACCGGTAGTGAGCTCCGGCTTCATGTGTGCAAAAGGGTGGCGAAACAAAACACGATTCAACCCATCAGGTAGCCCTACATACGTAAGAGAGTACAGGCACAAGGCTCCTGCAAGTCTCCTAGAAGGAAGAGGAGTTGAAAGTAGTTCTCACGTTGATATAATGGGAAATGTTAATCTTATTGAAGATATCTTGAGGGTTGCGGCTGGAGCCACTGGAGCAGAGATTGGTGGTGACAAGATTTACTCAGATATATTAACACTATCAGAGAGAATAAATCTTAAGCTGTGA

Protein sequence

MASVLRLRKLYFVNPVKSSSMGVQPIEAHKSEDKHNVIVAYVKKLMQMKQDKDKKKKRNKNIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGISGLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCAKHIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSNDSKRKKAFQEKEPVHYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMNRESIRKVSVNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNVNLIEDILRVAAGATGAEIGGDKIYSDILTLSERINLKL
Homology
BLAST of Lsi10G012050 vs. ExPASy Swiss-Prot
Match: Q9FYC7 (Putative phospholipid:diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=PDAT2 PE=3 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 2.4e-255
Identity = 433/662 (65.41%), Postives = 520/662 (78.55%), Query Frame = 0

Query: 43  KKLMQMKQD----KDKKKKRNKNIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLP 102
           +KL    +D    K K+    +  K+ +SG   C+D+CCW+IGY+CT WWL+ FLYH +P
Sbjct: 8   RKLSSFSEDTINPKPKQSATVEKPKRRRSGRCSCVDSCCWLIGYLCTAWWLLLFLYHSVP 67

Query: 103 LTLPGFRAIDSPGAVLKREGISGLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSF 162
           +     +A +SPG  L R+G+   HPV+LVPGIVTGGLELW+G+PCAEGLFRKRLWG SF
Sbjct: 68  VPAM-LQAPESPGTRLSRDGVKAFHPVILVPGIVTGGLELWEGRPCAEGLFRKRLWGASF 127

Query: 163 TETLKRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGY 222
           +E L+RPLCWLEHLSLD+ETGLDP GIRVR V GLVAADYFA  YF WAVLIENLAKIGY
Sbjct: 128 SEILRRPLCWLEHLSLDSETGLDPSGIRVRAVPGLVAADYFAPCYFAWAVLIENLAKIGY 187

Query: 223 DGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHF 282
           +GKNLHMA+YDWR++F NTEVRD++LSRLKSKIE+MY TNG  KVVVVPHSMG +YFLHF
Sbjct: 188 EGKNLHMASYDWRLSFHNTEVRDQSLSRLKSKIELMYATNGFKKVVVVPHSMGAIYFLHF 247

Query: 283 MKWVEAPPPMGGGGGGLGWCAKHIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTP 342
           +KWVE P P GGGGGG GWCAKHIK+++NIG  FLG PKAVS +LSAE + +A  R++ P
Sbjct: 248 LKWVETPLPDGGGGGGPGWCAKHIKSVVNIGPAFLGVPKAVSNLLSAEGKDIAYARSLAP 307

Query: 343 GFLKSEILGFQTLEHLLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNC--DLKKVDT 402
           G L SE+L  QTLEHL+R+S +WDS  SLLPKGGE IWGDLD   E+  NC    +K   
Sbjct: 308 GLLDSELLKLQTLEHLMRMSHSWDSIVSLLPKGGEAIWGDLDSHAEEGLNCIYSKRKSSQ 367

Query: 403 HSFRNENDSNDSKRKKAFQEKEPVHYGRIISFSKEAATRPSSELSTLNSEELKN-SGSSN 462
            S  N +  N S  K   + KEP  YGRI+SF K A+  PSS+LSTLN +EL    G+SN
Sbjct: 368 LSLSNLHKQNYS-LKPVSRVKEPAKYGRIVSFGKRASELPSSQLSTLNVKELSRVDGNSN 427

Query: 463 DSSFSCSDVWTEYDEMNRESIRKVSVNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAE 522
           DS+ SC + W+EY+EM+RESI KV+ N AYT  TV DLLR +APKMM+R ++HFSHGIA+
Sbjct: 428 DST-SCGEFWSEYNEMSRESIVKVAENTAYTATTVLDLLRFIAPKMMRRAEAHFSHGIAD 487

Query: 523 DLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYD--SSYDKCK-SIPF 582
           DLDDPKY HYKYWSNPLETKLP+AP+ME+YCLYGVGIPTERSYIY   +S  KCK SIPF
Sbjct: 488 DLDDPKYGHYKYWSNPLETKLPEAPEMEMYCLYGVGIPTERSYIYKLATSSGKCKSSIPF 547

Query: 583 RIDLSIEGKGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRH 642
           RID S++G    CL+ G  F DGD+SVPV+S+GFMCAKGWR KTRFNPSG  T++REY+H
Sbjct: 548 RIDGSLDG-DDVCLKGGTRFADGDESVPVISAGFMCAKGWRGKTRFNPSGMDTFLREYKH 607

Query: 643 KAPASLLEGRGVESSSHVDIMGNVNLIEDILRVAAGATGAEIGGDKIYSDILTLSERINL 695
           K P SLLE RG ES +HVDIMGNV LIED+LR+AAGA+G EIGGD+IYSD++ +SERI++
Sbjct: 608 KPPGSLLESRGTESGAHVDIMGNVGLIEDVLRIAAGASGQEIGGDRIYSDVMRMSERISI 665

BLAST of Lsi10G012050 vs. ExPASy Swiss-Prot
Match: Q9FNA9 (Phospholipid:diacylglycerol acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=PDAT1 PE=2 SV=1)

HSP 1 Score: 788.1 bits (2034), Expect = 8.1e-227
Identity = 383/661 (57.94%), Postives = 478/661 (72.31%), Query Frame = 0

Query: 47  QMKQDKDKKKK-----RNKNIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTL 106
           ++  D+D +KK     ++ + K    G+W C+D+CCW IG +C  WW + FLY+ +P + 
Sbjct: 20  EVVHDEDSQKKPHESSKSHHKKSNGGGKWSCIDSCCWFIGCVCVTWWFLLFLYNAMPASF 79

Query: 107 PGF-------RAIDSPGAVLKREGISGLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLW 166
           P +          D PG  LK+EG+   HPVV +PGIVTGGLELW+GK CA+GLFRKRLW
Sbjct: 80  PQYVTERITGPLPDPPGVKLKKEGLKAKHPVVFIPGIVTGGLELWEGKQCADGLFRKRLW 139

Query: 167 GGSFTETLKRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLA 226
           GG+F E  KRPLCW+EH+SLDNETGLDP GIRVR V GLVAADYFA GYFVWAVLI NLA
Sbjct: 140 GGTFGEVYKRPLCWVEHMSLDNETGLDPAGIRVRAVSGLVAADYFAPGYFVWAVLIANLA 199

Query: 227 KIGYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLY 286
            IGY+ KN++MAAYDWR++FQNTEVRD+ LSR+KS IE+M  TNGG K V+VPHSMGVLY
Sbjct: 200 HIGYEEKNMYMAAYDWRLSFQNTEVRDQTLSRMKSNIELMVSTNGGKKAVIVPHSMGVLY 259

Query: 287 FLHFMKWVEAPPPMGGGGGGLGWCAKHIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLR 346
           FLHFMKWVEAP P+GGGGG   WCAK+IKA+MNIG  FLG PKAV+ + SAE + VA+ R
Sbjct: 260 FLHFMKWVEAPAPLGGGGGP-DWCAKYIKAVMNIGGPFLGVPKAVAGLFSAEAKDVAVAR 319

Query: 347 AMTPGFLKSEILGFQTLEHLLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKV 406
           A+ PGFL ++I   QTL+H++R++RTWDST S+LPKGG+TIWG LDWSPE    C  KK 
Sbjct: 320 AIAPGFLDTDIFRLQTLQHVMRMTRTWDSTMSMLPKGGDTIWGGLDWSPEKGHTCCGKK- 379

Query: 407 DTHSFRNENDSNDSKRKKAFQEKEPVHYGRIISFSKEAATRPSSELSTLNSEELKNSGSS 466
                +  N++     +    +K PV+YGR+ISF KE A    SE++ ++        S 
Sbjct: 380 -----QKNNETCGEAGENGVSKKSPVNYGRMISFGKEVAEAAPSEINNIDFRGAVKGQSI 439

Query: 467 NDSSFSCSDVWTEYDEMNRESIRKVSVNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIA 526
              + +C DVWTEY +M    I+ ++  K YT     DLL  VAPKMM R  +HFS+GIA
Sbjct: 440 --PNHTCRDVWTEYHDMGIAGIKAIAEYKVYTAGEAIDLLHYVAPKMMARGAAHFSYGIA 499

Query: 527 EDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRI 586
           +DLDD KY   KYWSNPLETKLP+AP+MEIY LYGVGIPTER+Y+Y  +      IPF+I
Sbjct: 500 DDLDDTKYQDPKYWSNPLETKLPNAPEMEIYSLYGVGIPTERAYVYKLNQSPDSCIPFQI 559

Query: 587 DLSI-EGKGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHK 646
             S  E     CL++G+Y VDGD++VPV+S+G+MCAK WR KTRFNPSG  TY+REY H 
Sbjct: 560 FTSAHEEDEDSCLKAGVYNVDGDETVPVLSAGYMCAKAWRGKTRFNPSGIKTYIREYNHS 619

Query: 647 APASLLEGRGVESSSHVDIMGNVNLIEDILRVAAGATGAEIGGDKIYSDILTLSERINLK 695
            PA+LLEGRG +S +HVDIMGN  LIEDI+RVAAG  G++IG D+++S I   SERI+LK
Sbjct: 620 PPANLLEGRGTQSGAHVDIMGNFALIEDIMRVAAGGNGSDIGHDQVHSGIFEWSERIDLK 671

BLAST of Lsi10G012050 vs. ExPASy Swiss-Prot
Match: P40345 (Phospholipid:diacylglycerol acyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=LRO1 PE=1 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 2.5e-82
Identity = 182/565 (32.21%), Postives = 284/565 (50.27%), Query Frame = 0

Query: 113 VLKREGISGLHPVVLVPGIVTGGLELW----DGKPCAEGLFRKRLWGGSF--TETLKRPL 172
           +L+   I   HPVV+VPG+++ G+E W    D +  +   FRKRLWG  +     +   +
Sbjct: 163 LLRDYNIEAKHPVVMVPGVISTGIESWGVIGDDECDSSAHFRKRLWGSFYMLRTMVMDKV 222

Query: 173 CWLEHLSLDNETGLDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMA 232
           CWL+H+ LD ETGLDPP   +R  +G  + DYF  GY++W  + +NL  IGY+   +  A
Sbjct: 223 CWLKHVMLDPETGLDPPNFTLRAAQGFESTDYFIAGYWIWNKVFQNLGVIGYEPNKMTSA 282

Query: 233 AYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPP 292
           AYDWR+A+ + E RDR  ++LK +IE+ ++ + G KV ++ HSMG     +FMKWVEA  
Sbjct: 283 AYDWRLAYLDLERRDRYFTKLKEQIELFHQLS-GEKVCLIGHSMGSQIIFYFMKWVEAEG 342

Query: 293 PMGGGGGGLGWCAKHIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEIL 352
           P+  G GG GW  +HI + +N   T LG PKAV  ++S E +    L  +    L+    
Sbjct: 343 PL-YGNGGRGWVNEHIDSFINAAGTLLGAPKAVPALISGEMKDTIQLNTLAMYGLEK--- 402

Query: 353 GFQTLEHLLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSN 412
            F  +E  +++ +TW    S+LPKG E IWGD+  S ED  N            N  D+ 
Sbjct: 403 FFSRIER-VKMLQTWGGIPSMLPKGEEVIWGDMKSSSEDALN------------NNTDT- 462

Query: 413 DSKRKKAFQEKEPVHYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWT 472
                          YG  I F +  +                                 
Sbjct: 463 ---------------YGNFIRFERNTSD-------------------------------- 522

Query: 473 EYDEMNRESIRKVSVNKAYTVKTVFDLLRVVAPKMMQ-RMDSHFSHGIAEDLDD--PKYA 532
                        + NK  T+K   ++   ++P+ +Q R+   +S G +++ ++      
Sbjct: 523 -------------AFNKNLTMKDAINMTLSISPEWLQRRVHEQYSFGYSKNEEELRKNEL 582

Query: 533 HYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGS 592
           H+K+WSNP+E  LP+AP M+IYC+YGV  PTER+Y+Y    D   S    + +  E K  
Sbjct: 583 HHKHWSNPMEVPLPEAPHMKIYCIYGVNNPTERAYVYKEEDD---SSALNLTIDYESK-- 637

Query: 593 GCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRG 652
                 ++  +GD +VP+V+   MC K  +  + +NP+G    + E +H+     + G G
Sbjct: 643 ----QPVFLTEGDGTVPLVAHS-MCHKWAQGASPYNPAGINVTIVEMKHQPDRFDIRG-G 637

Query: 653 VESSSHVDIMGNVNLIEDILRVAAG 669
            +S+ HVDI+G+  L + IL++A+G
Sbjct: 703 AKSAEHVDILGSAELNDYILKIASG 637

BLAST of Lsi10G012050 vs. ExPASy Swiss-Prot
Match: O94680 (Phospholipid:diacylglycerol acyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=plh1 PE=1 SV=2)

HSP 1 Score: 307.8 bits (787), Expect = 3.2e-82
Identity = 199/588 (33.84%), Postives = 294/588 (50.00%), Query Frame = 0

Query: 111 GAVLKREGISGLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWG--GSFTETLKRPLCW 170
           G  +  EG    HPV++VPG+++ GLE W    C+   FRKRLWG             CW
Sbjct: 134 GLDMYNEGYRSDHPVIMVPGVISSGLESWSFNNCSIPYFRKRLWGSWSMLKAMFLDKQCW 193

Query: 171 LEHLSLDNETGLDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAY 230
           LEHL LD +TGLDP GI++R  +G  AAD+F  GY++W+ +IENLA IGY+  N+  A+Y
Sbjct: 194 LEHLMLDKKTGLDPKGIKLRAAQGFEAADFFITGYWIWSKVIENLAAIGYEPNNMLSASY 253

Query: 231 DWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPM 290
           DWR+++ N E RD+  S+LK  IE     +   KVV++ HSMG     +F KWVEA    
Sbjct: 254 DWRLSYANLEERDKYFSKLKMFIEYSNIVH-KKKVVLISHSMGSQVTYYFFKWVEAE--- 313

Query: 291 GGGGGGLGWCAKHIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGF 350
           G G GG  W   HI+A +NI  + +G PK V+ +LS E +  A L   +   L+     F
Sbjct: 314 GYGNGGPTWVNDHIEAFINISGSLIGAPKTVAALLSGEMKDTAQLNQFSVYGLEK---FF 373

Query: 351 QTLEHLLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSNDS 410
              E  + V RT    +S+LPKGG+ +WG+  W+P+D+                N +N S
Sbjct: 374 SRSERAMMV-RTMGGVSSMLPKGGDVVWGNASWAPDDL----------------NQTNFS 433

Query: 411 KRKKAFQEKEPVHYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEY 470
                         G II + +                                D+  ++
Sbjct: 434 N-------------GAIIRYRE--------------------------------DIDKDH 493

Query: 471 DEMNRESIRKVSVNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIA----EDLDDPKYAH 530
           DE + +   +   N         D  +V+  K       ++SHG+A    E L + +   
Sbjct: 494 DEFDIDDALQFLKN------VTDDDFKVMLAK-------NYSHGLAWTEKEVLKNNEMP- 553

Query: 531 YKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSG 590
              W NPLET LP APDM+IYC++GVG PTER Y Y ++ +    I   ++   +     
Sbjct: 554 -SKWINPLETSLPYAPDMKIYCVHGVGKPTERGYYYTNNPEGQPVIDSSVNDGTK----- 613

Query: 591 CLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGV 650
            + +G+   DGD ++P+++ G +C K W+ K RFNP+ +     E +H+  A  L G G 
Sbjct: 614 -VENGIVMDDGDGTLPILALGLVCNKVWQTK-RFNPANTSITNYEIKHEPAAFDLRG-GP 627

Query: 651 ESSSHVDIMGNVNLIEDILRVAAGATGAEIGGDKIYSDILTLSERINL 693
            S+ HVDI+G+  L E IL+V++G    +   ++  SDI  +   INL
Sbjct: 674 RSAEHVDILGHSELNEIILKVSSG--HGDSVPNRYISDIQEIINEINL 627

BLAST of Lsi10G012050 vs. ExPASy Swiss-Prot
Match: Q8WMP9 (Phospholipase A2 group XV OS=Bos taurus OX=9913 GN=PLA2G15 PE=1 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 3.2e-13
Identity = 73/259 (28.19%), Postives = 116/259 (44.79%), Query Frame = 0

Query: 124 PVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPL-------CWLEHLSL-- 183
           PVVLVPG +   LE    KP        +     FT  L   L       CW++++ L  
Sbjct: 34  PVVLVPGDMGNQLEAKLDKPSVVHYVCSKRTDHYFTLWLNLELLLPVIIDCWIDNVRLIY 93

Query: 184 --DNETGLDPPGIRVRPVEGLVAADYFAQGYF---------VWAVLIENLAKIGYD-GKN 243
              + T   P G+ VR V G    D F+  +              ++E+L   GY+ GK+
Sbjct: 94  NQTSHTTQFPEGVDVR-VPGF--GDTFSMEFLDPSKSSVGSYLHTMVESLVSWGYERGKD 153

Query: 244 LHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWV 303
           +  A YDWR A         AL ++   IE MY+  GG  VV+V HSMG +Y L+F++  
Sbjct: 154 VRGAPYDWRRAPNENGPYFLALRKM---IEEMYQLYGG-PVVLVAHSMGNMYMLYFLQ-- 213

Query: 304 EAPPPMGGGGGGLGWCAKHIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLK 362
             P           W  K+I+A + +G  + G PK +  + S ++  + ++R++    ++
Sbjct: 214 HQPQ---------DWKDKYIRAFVALGPPWGGVPKTLRVLASGDNNRIPVIRSLK---IR 271

BLAST of Lsi10G012050 vs. ExPASy TrEMBL
Match: A0A5A7SR19 (Putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold26G00090 PE=4 SV=1)

HSP 1 Score: 1344.3 bits (3478), Expect = 0.0e+00
Identity = 657/694 (94.67%), Postives = 673/694 (96.97%), Query Frame = 0

Query: 1   MASVLRLRKLYFVNPVKSSSMGVQPIEAHKSEDKHNVIVAYVKKLMQMKQDKDKKKKRNK 60
           MASVLRLRKLYFVNPVKSSSMG+Q IEAHKSEDK NVIVAYVKKLMQ KQDKD K+KRNK
Sbjct: 1   MASVLRLRKLYFVNPVKSSSMGLQSIEAHKSEDKRNVIVAYVKKLMQKKQDKDDKQKRNK 60

Query: 61  NIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGIS 120
           N  +GQS EWKCLDNCCWIIGY+CTFWWLMFFLYHYLPL++PGF AIDSPGAVLKREGIS
Sbjct: 61  NTNRGQSREWKCLDNCCWIIGYICTFWWLMFFLYHYLPLSVPGFPAIDSPGAVLKREGIS 120

Query: 121 GLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180
           G HPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL
Sbjct: 121 GRHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180

Query: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240
           DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR
Sbjct: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240

Query: 241 DRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCAK 300
           DRALSRLKSKIEVMY TNG NKVVVVPHSMGVLYFLHFMKWVEAPPPM GGGGGLGWCAK
Sbjct: 241 DRALSRLKSKIEVMYATNGENKVVVVPHSMGVLYFLHFMKWVEAPPPM-GGGGGLGWCAK 300

Query: 301 HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 360
           HIK+IMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT
Sbjct: 301 HIKSIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 360

Query: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSNDSKRKKAFQEKEPV 420
           WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVD HSFRNENDS+DS+RKK FQE+EPV
Sbjct: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDAHSFRNENDSDDSERKKCFQEQEPV 420

Query: 421 HYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMNRESIRKVS 480
           HYGRIISFSKEAATRPSSELSTLN EELKNSG SNDSSFSCSDVWTEYDEM+R+SIRKVS
Sbjct: 421 HYGRIISFSKEAATRPSSELSTLNLEELKNSGGSNDSSFSCSDVWTEYDEMSRDSIRKVS 480

Query: 481 VNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDAP 540
            NKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAH+KYWSNPLETKLPDAP
Sbjct: 481 ENKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHHKYWSNPLETKLPDAP 540

Query: 541 DMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP 600
           +MEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP
Sbjct: 541 NMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP 600

Query: 601 VVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNVNLIE 660
           +VSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESS+HVDIMGNVNLIE
Sbjct: 601 IVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSAHVDIMGNVNLIE 660

Query: 661 DILRVAAGATGAEIGGDKIYSDILTLSERINLKL 695
           DILRVAA   G EIGGDKIYSDILTLSERINLKL
Sbjct: 661 DILRVAA---GEEIGGDKIYSDILTLSERINLKL 690

BLAST of Lsi10G012050 vs. ExPASy TrEMBL
Match: A0A1S3C9K8 (putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucumis melo OX=3656 GN=LOC103497986 PE=4 SV=1)

HSP 1 Score: 1344.3 bits (3478), Expect = 0.0e+00
Identity = 657/694 (94.67%), Postives = 673/694 (96.97%), Query Frame = 0

Query: 1   MASVLRLRKLYFVNPVKSSSMGVQPIEAHKSEDKHNVIVAYVKKLMQMKQDKDKKKKRNK 60
           MASVLRLRKLYFVNPVKSSSMG+Q IEAHKSEDK NVIVAYVKKLMQ KQDKD K+KRNK
Sbjct: 114 MASVLRLRKLYFVNPVKSSSMGLQSIEAHKSEDKRNVIVAYVKKLMQKKQDKDDKQKRNK 173

Query: 61  NIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGIS 120
           N  +GQS EWKCLDNCCWIIGY+CTFWWLMFFLYHYLPL++PGF AIDSPGAVLKREGIS
Sbjct: 174 NTNRGQSREWKCLDNCCWIIGYICTFWWLMFFLYHYLPLSVPGFPAIDSPGAVLKREGIS 233

Query: 121 GLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180
           G HPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL
Sbjct: 234 GRHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 293

Query: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240
           DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR
Sbjct: 294 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 353

Query: 241 DRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCAK 300
           DRALSRLKSKIEVMY TNG NKVVVVPHSMGVLYFLHFMKWVEAPPPM GGGGGLGWCAK
Sbjct: 354 DRALSRLKSKIEVMYATNGENKVVVVPHSMGVLYFLHFMKWVEAPPPM-GGGGGLGWCAK 413

Query: 301 HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 360
           HIK+IMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT
Sbjct: 414 HIKSIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 473

Query: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSNDSKRKKAFQEKEPV 420
           WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVD HSFRNENDS+DS+RKK FQE+EPV
Sbjct: 474 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDAHSFRNENDSDDSERKKCFQEQEPV 533

Query: 421 HYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMNRESIRKVS 480
           HYGRIISFSKEAATRPSSELSTLN EELKNSG SNDSSFSCSDVWTEYDEM+R+SIRKVS
Sbjct: 534 HYGRIISFSKEAATRPSSELSTLNLEELKNSGGSNDSSFSCSDVWTEYDEMSRDSIRKVS 593

Query: 481 VNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDAP 540
            NKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAH+KYWSNPLETKLPDAP
Sbjct: 594 ENKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHHKYWSNPLETKLPDAP 653

Query: 541 DMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP 600
           +MEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP
Sbjct: 654 NMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP 713

Query: 601 VVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNVNLIE 660
           +VSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESS+HVDIMGNVNLIE
Sbjct: 714 IVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSAHVDIMGNVNLIE 773

Query: 661 DILRVAAGATGAEIGGDKIYSDILTLSERINLKL 695
           DILRVAA   G EIGGDKIYSDILTLSERINLKL
Sbjct: 774 DILRVAA---GEEIGGDKIYSDILTLSERINLKL 803

BLAST of Lsi10G012050 vs. ExPASy TrEMBL
Match: A0A0A0KGM0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G149960 PE=4 SV=1)

HSP 1 Score: 1343.9 bits (3477), Expect = 0.0e+00
Identity = 657/694 (94.67%), Postives = 672/694 (96.83%), Query Frame = 0

Query: 1   MASVLRLRKLYFVNPVKSSSMGVQPIEAHKSEDKHNVIVAYVKKLMQMKQDKDKKKKRNK 60
           MASVLRLRKLYFVNPVKSSSMG+Q IEAHKS++K NVIVAYVK LMQ KQDKD KKKRNK
Sbjct: 1   MASVLRLRKLYFVNPVKSSSMGLQSIEAHKSDNKRNVIVAYVKNLMQKKQDKDDKKKRNK 60

Query: 61  NIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGIS 120
           NI +GQS EWKCLDNCCWIIGY+CTFWWLMFFLYHYLPL+LPGF  IDSPGAVLKREGIS
Sbjct: 61  NINRGQSREWKCLDNCCWIIGYICTFWWLMFFLYHYLPLSLPGFPVIDSPGAVLKREGIS 120

Query: 121 GLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180
           G HPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL
Sbjct: 121 GRHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180

Query: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240
           DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR
Sbjct: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240

Query: 241 DRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCAK 300
           DRALSRLKSKIEVMY TNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPM GGGGGLGWCAK
Sbjct: 241 DRALSRLKSKIEVMYATNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPM-GGGGGLGWCAK 300

Query: 301 HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 360
           HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT
Sbjct: 301 HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 360

Query: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSNDSKRKKAFQEKEPV 420
           WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVD HSFRNENDS+DS+RKK FQE+EPV
Sbjct: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDAHSFRNENDSDDSERKKCFQEQEPV 420

Query: 421 HYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMNRESIRKVS 480
           HYGRIISFSKEAATRPSSELST N EELKNSG SNDSSFSCSDVWTEYDEM+RESIRKVS
Sbjct: 421 HYGRIISFSKEAATRPSSELSTQNLEELKNSGGSNDSSFSCSDVWTEYDEMSRESIRKVS 480

Query: 481 VNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDAP 540
            NKAYTV+TVFDLLR+VAPKMMQRMDSHFSHGIAEDLDDPKYAH+KYWSNPLETKLPDAP
Sbjct: 481 ENKAYTVETVFDLLRIVAPKMMQRMDSHFSHGIAEDLDDPKYAHHKYWSNPLETKLPDAP 540

Query: 541 DMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP 600
           DMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP
Sbjct: 541 DMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP 600

Query: 601 VVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNVNLIE 660
           VVSSGFMCAKGWRNKTRFNPSGSPT+VREYRHKAPASLLEGRGVESS+HVDIMGNVNLIE
Sbjct: 601 VVSSGFMCAKGWRNKTRFNPSGSPTHVREYRHKAPASLLEGRGVESSAHVDIMGNVNLIE 660

Query: 661 DILRVAAGATGAEIGGDKIYSDILTLSERINLKL 695
           DILRVAA   G EIGGDKIYSDILTLSERINLKL
Sbjct: 661 DILRVAA---GEEIGGDKIYSDILTLSERINLKL 690

BLAST of Lsi10G012050 vs. ExPASy TrEMBL
Match: A0A6J1JAN3 (putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucurbita maxima OX=3661 GN=LOC111484072 PE=4 SV=1)

HSP 1 Score: 1335.5 bits (3455), Expect = 0.0e+00
Identity = 645/695 (92.81%), Postives = 673/695 (96.83%), Query Frame = 0

Query: 1   MASVLRLRKLYFVNPVKSSSMGVQPI-EAHKSEDKHNVIVAYVKKLMQMKQDKDKKKKRN 60
           MASVLRLRKLY+VNPVK+SSMG+Q I +AHKSEDK NV+VAYVKK++Q KQDKD+KKKRN
Sbjct: 1   MASVLRLRKLYYVNPVKNSSMGIQTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRN 60

Query: 61  KNIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGI 120
           KNI KGQS EWKCLDNCCWIIGYMCTFWWLMFF+YHYLPLTLPGF AIDSPGAVLKR+G+
Sbjct: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           SGLHPVVLVPGIVTGGLELWDGKPCA+GLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG
Sbjct: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180

Query: 181 LDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIR+RPVEGLVAADYF QGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV
Sbjct: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240

Query: 241 RDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCA 300
           RDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPM GGGGGLGWCA
Sbjct: 241 RDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPM-GGGGGLGWCA 300

Query: 301 KHIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSR 360
           K+IKAIMNIGSTFLGTPKAVSKILSAEDRH+ALLRAMTPGFLKSEILGFQTLEH+LRVSR
Sbjct: 301 KYIKAIMNIGSTFLGTPKAVSKILSAEDRHIALLRAMTPGFLKSEILGFQTLEHMLRVSR 360

Query: 361 TWDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSNDSKRKKAFQEKEP 420
           TWDSTASLLPKGGETIWGDLDWSPEDVQNCDL+KVDTHSFRNENDSNDS+ KK  QEKE 
Sbjct: 361 TWDSTASLLPKGGETIWGDLDWSPEDVQNCDLEKVDTHSFRNENDSNDSEIKKGLQEKEA 420

Query: 421 VHYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMNRESIRKV 480
           VHYGR+ISF KEAATRPSSELSTL SEEL +SGSSNDSSFSCSD WTEYDEM+RES+RKV
Sbjct: 421 VHYGRMISFGKEAATRPSSELSTLYSEELMDSGSSNDSSFSCSDGWTEYDEMSRESLRKV 480

Query: 481 SVNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDA 540
           S NKAYT KTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDA
Sbjct: 481 SENKAYTAKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDA 540

Query: 541 PDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSV 600
           PDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLS++G+GSGCLRSGMYFVDGDD V
Sbjct: 541 PDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSVDGEGSGCLRSGMYFVDGDDCV 600

Query: 601 PVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNVNLI 660
           PVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRG+ESS+HVDIMGN  LI
Sbjct: 601 PVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGIESSAHVDIMGNAKLI 660

Query: 661 EDILRVAAGATGAEIGGDKIYSDILTLSERINLKL 695
           EDILRVAAGATGAEIGGD+IYSDILTLSERINLKL
Sbjct: 661 EDILRVAAGATGAEIGGDRIYSDILTLSERINLKL 694

BLAST of Lsi10G012050 vs. ExPASy TrEMBL
Match: A0A6J1FW53 (putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucurbita moschata OX=3662 GN=LOC111448684 PE=4 SV=1)

HSP 1 Score: 1332.8 bits (3448), Expect = 0.0e+00
Identity = 643/695 (92.52%), Postives = 672/695 (96.69%), Query Frame = 0

Query: 1   MASVLRLRKLYFVNPVKSSSMGVQPI-EAHKSEDKHNVIVAYVKKLMQMKQDKDKKKKRN 60
           MASVLRLRKLY+VNPVK+SSMG+Q I +AHKSEDK NV+VAYVKK++Q KQDKD+KKKRN
Sbjct: 1   MASVLRLRKLYYVNPVKNSSMGIQTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRN 60

Query: 61  KNIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGI 120
           KNI KGQS EWKCLDNCCWIIGYMCTFWWLMFF+YHYLPLTLPGF AIDSPGAVLKR+G+
Sbjct: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           SGLHPVVLVPGIVTGGLELWDGKPCA+GLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG
Sbjct: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180

Query: 181 LDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIR+RPVEGLVAADYF QGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV
Sbjct: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240

Query: 241 RDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCA 300
           RDRALSRLKSKIEVMYETNGGN+VVVVPHSMGVLYFLHFMKWVEAPPPM GGGGGLGWCA
Sbjct: 241 RDRALSRLKSKIEVMYETNGGNRVVVVPHSMGVLYFLHFMKWVEAPPPM-GGGGGLGWCA 300

Query: 301 KHIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSR 360
           K+IKAIMNIGSTFLGTPKAVSKILSAEDRH+ALLRAMTPGFLKSEILGFQTLEH+LRVSR
Sbjct: 301 KYIKAIMNIGSTFLGTPKAVSKILSAEDRHIALLRAMTPGFLKSEILGFQTLEHMLRVSR 360

Query: 361 TWDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSNDSKRKKAFQEKEP 420
           TWDSTASLLPKGGETIWGDLDWSPEDVQNCDL+K DTHSFRNENDSNDS+ KK FQEKE 
Sbjct: 361 TWDSTASLLPKGGETIWGDLDWSPEDVQNCDLEKADTHSFRNENDSNDSEIKKGFQEKEA 420

Query: 421 VHYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMNRESIRKV 480
           VHYGR+ISF KEAATRPSSELSTL SEEL +SGSSND+SFSCSD WTEYDEM+RES+RKV
Sbjct: 421 VHYGRMISFGKEAATRPSSELSTLYSEELMDSGSSNDTSFSCSDGWTEYDEMSRESLRKV 480

Query: 481 SVNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDA 540
           S NKAYT KTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKY HYKYWSNPLETKLPDA
Sbjct: 481 SENKAYTAKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYGHYKYWSNPLETKLPDA 540

Query: 541 PDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSV 600
           PDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSI+G+GSGCLRSGMYFVDGDD V
Sbjct: 541 PDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIDGEGSGCLRSGMYFVDGDDCV 600

Query: 601 PVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNVNLI 660
           PVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRG+ESS+HVDIMGN  LI
Sbjct: 601 PVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGIESSAHVDIMGNAKLI 660

Query: 661 EDILRVAAGATGAEIGGDKIYSDILTLSERINLKL 695
           EDILRVAAGATGAEIGGD+IYSDILTLSERINLKL
Sbjct: 661 EDILRVAAGATGAEIGGDRIYSDILTLSERINLKL 694

BLAST of Lsi10G012050 vs. NCBI nr
Match: XP_038902104.1 (putative phospholipid:diacylglycerol acyltransferase 2 [Benincasa hispida])

HSP 1 Score: 1376.7 bits (3562), Expect = 0.0e+00
Identity = 673/694 (96.97%), Postives = 680/694 (97.98%), Query Frame = 0

Query: 1   MASVLRLRKLYFVNPVKSSSMGVQPIEAHKSEDKHNVIVAYVKKLMQMKQDKDKKKKRNK 60
           MASVLRLRKLYFVNPVKSSSMGVQPIEAHKSEDKHNVIVAYVKKLMQ KQD+DKKKK NK
Sbjct: 1   MASVLRLRKLYFVNPVKSSSMGVQPIEAHKSEDKHNVIVAYVKKLMQKKQDEDKKKKGNK 60

Query: 61  NIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGIS 120
           NI K QS EWKCLDNCCWIIGY+CTFWWLMFFLYHYLPLTLPGF AIDSPGAVLKREGIS
Sbjct: 61  NIIKRQSREWKCLDNCCWIIGYICTFWWLMFFLYHYLPLTLPGFPAIDSPGAVLKREGIS 120

Query: 121 GLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180
           GLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL
Sbjct: 121 GLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180

Query: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240
           DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR
Sbjct: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240

Query: 241 DRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCAK 300
           DRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLY+LHFMKWVEAPPPMGGGGG  GWCAK
Sbjct: 241 DRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYYLHFMKWVEAPPPMGGGGGH-GWCAK 300

Query: 301 HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 360
           HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEH+LRVSRT
Sbjct: 301 HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHMLRVSRT 360

Query: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSNDSKRKKAFQEKEPV 420
           WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDS DS+RKK FQE EPV
Sbjct: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSKDSERKKVFQENEPV 420

Query: 421 HYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMNRESIRKVS 480
           HYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEM+RESIRKVS
Sbjct: 421 HYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMSRESIRKVS 480

Query: 481 VNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDAP 540
            NKAYTVKTVFDLLRVVAPKMMQRMD HFSHGIAEDLDDP YAHYKYWSNPLETKLPDAP
Sbjct: 481 ENKAYTVKTVFDLLRVVAPKMMQRMDGHFSHGIAEDLDDPTYAHYKYWSNPLETKLPDAP 540

Query: 541 DMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP 600
           DMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP
Sbjct: 541 DMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP 600

Query: 601 VVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNVNLIE 660
           VVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESS+HVDIMGNVNLIE
Sbjct: 601 VVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSAHVDIMGNVNLIE 660

Query: 661 DILRVAAGATGAEIGGDKIYSDILTLSERINLKL 695
           DILRVAAGATGAEIGGDKIYSDILTLSERINLKL
Sbjct: 661 DILRVAAGATGAEIGGDKIYSDILTLSERINLKL 693

BLAST of Lsi10G012050 vs. NCBI nr
Match: XP_008458650.1 (PREDICTED: putative phospholipid:diacylglycerol acyltransferase 2 [Cucumis melo])

HSP 1 Score: 1344.3 bits (3478), Expect = 0.0e+00
Identity = 657/694 (94.67%), Postives = 673/694 (96.97%), Query Frame = 0

Query: 1   MASVLRLRKLYFVNPVKSSSMGVQPIEAHKSEDKHNVIVAYVKKLMQMKQDKDKKKKRNK 60
           MASVLRLRKLYFVNPVKSSSMG+Q IEAHKSEDK NVIVAYVKKLMQ KQDKD K+KRNK
Sbjct: 114 MASVLRLRKLYFVNPVKSSSMGLQSIEAHKSEDKRNVIVAYVKKLMQKKQDKDDKQKRNK 173

Query: 61  NIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGIS 120
           N  +GQS EWKCLDNCCWIIGY+CTFWWLMFFLYHYLPL++PGF AIDSPGAVLKREGIS
Sbjct: 174 NTNRGQSREWKCLDNCCWIIGYICTFWWLMFFLYHYLPLSVPGFPAIDSPGAVLKREGIS 233

Query: 121 GLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180
           G HPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL
Sbjct: 234 GRHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 293

Query: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240
           DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR
Sbjct: 294 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 353

Query: 241 DRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCAK 300
           DRALSRLKSKIEVMY TNG NKVVVVPHSMGVLYFLHFMKWVEAPPPM GGGGGLGWCAK
Sbjct: 354 DRALSRLKSKIEVMYATNGENKVVVVPHSMGVLYFLHFMKWVEAPPPM-GGGGGLGWCAK 413

Query: 301 HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 360
           HIK+IMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT
Sbjct: 414 HIKSIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 473

Query: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSNDSKRKKAFQEKEPV 420
           WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVD HSFRNENDS+DS+RKK FQE+EPV
Sbjct: 474 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDAHSFRNENDSDDSERKKCFQEQEPV 533

Query: 421 HYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMNRESIRKVS 480
           HYGRIISFSKEAATRPSSELSTLN EELKNSG SNDSSFSCSDVWTEYDEM+R+SIRKVS
Sbjct: 534 HYGRIISFSKEAATRPSSELSTLNLEELKNSGGSNDSSFSCSDVWTEYDEMSRDSIRKVS 593

Query: 481 VNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDAP 540
            NKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAH+KYWSNPLETKLPDAP
Sbjct: 594 ENKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHHKYWSNPLETKLPDAP 653

Query: 541 DMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP 600
           +MEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP
Sbjct: 654 NMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP 713

Query: 601 VVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNVNLIE 660
           +VSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESS+HVDIMGNVNLIE
Sbjct: 714 IVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSAHVDIMGNVNLIE 773

Query: 661 DILRVAAGATGAEIGGDKIYSDILTLSERINLKL 695
           DILRVAA   G EIGGDKIYSDILTLSERINLKL
Sbjct: 774 DILRVAA---GEEIGGDKIYSDILTLSERINLKL 803

BLAST of Lsi10G012050 vs. NCBI nr
Match: KAA0033360.1 (putative phospholipid:diacylglycerol acyltransferase 2 [Cucumis melo var. makuwa] >TYJ96644.1 putative phospholipid:diacylglycerol acyltransferase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1344.3 bits (3478), Expect = 0.0e+00
Identity = 657/694 (94.67%), Postives = 673/694 (96.97%), Query Frame = 0

Query: 1   MASVLRLRKLYFVNPVKSSSMGVQPIEAHKSEDKHNVIVAYVKKLMQMKQDKDKKKKRNK 60
           MASVLRLRKLYFVNPVKSSSMG+Q IEAHKSEDK NVIVAYVKKLMQ KQDKD K+KRNK
Sbjct: 1   MASVLRLRKLYFVNPVKSSSMGLQSIEAHKSEDKRNVIVAYVKKLMQKKQDKDDKQKRNK 60

Query: 61  NIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGIS 120
           N  +GQS EWKCLDNCCWIIGY+CTFWWLMFFLYHYLPL++PGF AIDSPGAVLKREGIS
Sbjct: 61  NTNRGQSREWKCLDNCCWIIGYICTFWWLMFFLYHYLPLSVPGFPAIDSPGAVLKREGIS 120

Query: 121 GLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180
           G HPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL
Sbjct: 121 GRHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180

Query: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240
           DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR
Sbjct: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240

Query: 241 DRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCAK 300
           DRALSRLKSKIEVMY TNG NKVVVVPHSMGVLYFLHFMKWVEAPPPM GGGGGLGWCAK
Sbjct: 241 DRALSRLKSKIEVMYATNGENKVVVVPHSMGVLYFLHFMKWVEAPPPM-GGGGGLGWCAK 300

Query: 301 HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 360
           HIK+IMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT
Sbjct: 301 HIKSIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 360

Query: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSNDSKRKKAFQEKEPV 420
           WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVD HSFRNENDS+DS+RKK FQE+EPV
Sbjct: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDAHSFRNENDSDDSERKKCFQEQEPV 420

Query: 421 HYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMNRESIRKVS 480
           HYGRIISFSKEAATRPSSELSTLN EELKNSG SNDSSFSCSDVWTEYDEM+R+SIRKVS
Sbjct: 421 HYGRIISFSKEAATRPSSELSTLNLEELKNSGGSNDSSFSCSDVWTEYDEMSRDSIRKVS 480

Query: 481 VNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDAP 540
            NKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAH+KYWSNPLETKLPDAP
Sbjct: 481 ENKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHHKYWSNPLETKLPDAP 540

Query: 541 DMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP 600
           +MEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP
Sbjct: 541 NMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP 600

Query: 601 VVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNVNLIE 660
           +VSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESS+HVDIMGNVNLIE
Sbjct: 601 IVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSAHVDIMGNVNLIE 660

Query: 661 DILRVAAGATGAEIGGDKIYSDILTLSERINLKL 695
           DILRVAA   G EIGGDKIYSDILTLSERINLKL
Sbjct: 661 DILRVAA---GEEIGGDKIYSDILTLSERINLKL 690

BLAST of Lsi10G012050 vs. NCBI nr
Match: XP_004145827.1 (putative phospholipid:diacylglycerol acyltransferase 2 [Cucumis sativus] >KGN46906.1 hypothetical protein Csa_020935 [Cucumis sativus])

HSP 1 Score: 1343.9 bits (3477), Expect = 0.0e+00
Identity = 657/694 (94.67%), Postives = 672/694 (96.83%), Query Frame = 0

Query: 1   MASVLRLRKLYFVNPVKSSSMGVQPIEAHKSEDKHNVIVAYVKKLMQMKQDKDKKKKRNK 60
           MASVLRLRKLYFVNPVKSSSMG+Q IEAHKS++K NVIVAYVK LMQ KQDKD KKKRNK
Sbjct: 1   MASVLRLRKLYFVNPVKSSSMGLQSIEAHKSDNKRNVIVAYVKNLMQKKQDKDDKKKRNK 60

Query: 61  NIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGIS 120
           NI +GQS EWKCLDNCCWIIGY+CTFWWLMFFLYHYLPL+LPGF  IDSPGAVLKREGIS
Sbjct: 61  NINRGQSREWKCLDNCCWIIGYICTFWWLMFFLYHYLPLSLPGFPVIDSPGAVLKREGIS 120

Query: 121 GLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180
           G HPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL
Sbjct: 121 GRHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETGL 180

Query: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240
           DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR
Sbjct: 181 DPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEVR 240

Query: 241 DRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCAK 300
           DRALSRLKSKIEVMY TNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPM GGGGGLGWCAK
Sbjct: 241 DRALSRLKSKIEVMYATNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPM-GGGGGLGWCAK 300

Query: 301 HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 360
           HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT
Sbjct: 301 HIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSRT 360

Query: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSNDSKRKKAFQEKEPV 420
           WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVD HSFRNENDS+DS+RKK FQE+EPV
Sbjct: 361 WDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDAHSFRNENDSDDSERKKCFQEQEPV 420

Query: 421 HYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMNRESIRKVS 480
           HYGRIISFSKEAATRPSSELST N EELKNSG SNDSSFSCSDVWTEYDEM+RESIRKVS
Sbjct: 421 HYGRIISFSKEAATRPSSELSTQNLEELKNSGGSNDSSFSCSDVWTEYDEMSRESIRKVS 480

Query: 481 VNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDAP 540
            NKAYTV+TVFDLLR+VAPKMMQRMDSHFSHGIAEDLDDPKYAH+KYWSNPLETKLPDAP
Sbjct: 481 ENKAYTVETVFDLLRIVAPKMMQRMDSHFSHGIAEDLDDPKYAHHKYWSNPLETKLPDAP 540

Query: 541 DMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP 600
           DMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP
Sbjct: 541 DMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSVP 600

Query: 601 VVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNVNLIE 660
           VVSSGFMCAKGWRNKTRFNPSGSPT+VREYRHKAPASLLEGRGVESS+HVDIMGNVNLIE
Sbjct: 601 VVSSGFMCAKGWRNKTRFNPSGSPTHVREYRHKAPASLLEGRGVESSAHVDIMGNVNLIE 660

Query: 661 DILRVAAGATGAEIGGDKIYSDILTLSERINLKL 695
           DILRVAA   G EIGGDKIYSDILTLSERINLKL
Sbjct: 661 DILRVAA---GEEIGGDKIYSDILTLSERINLKL 690

BLAST of Lsi10G012050 vs. NCBI nr
Match: XP_022986276.1 (putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita maxima])

HSP 1 Score: 1335.5 bits (3455), Expect = 0.0e+00
Identity = 645/695 (92.81%), Postives = 673/695 (96.83%), Query Frame = 0

Query: 1   MASVLRLRKLYFVNPVKSSSMGVQPI-EAHKSEDKHNVIVAYVKKLMQMKQDKDKKKKRN 60
           MASVLRLRKLY+VNPVK+SSMG+Q I +AHKSEDK NV+VAYVKK++Q KQDKD+KKKRN
Sbjct: 1   MASVLRLRKLYYVNPVKNSSMGIQTIDDAHKSEDKPNVVVAYVKKMLQKKQDKDRKKKRN 60

Query: 61  KNIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTLPGFRAIDSPGAVLKREGI 120
           KNI KGQS EWKCLDNCCWIIGYMCTFWWLMFF+YHYLPLTLPGF AIDSPGAVLKR+G+
Sbjct: 61  KNINKGQSREWKCLDNCCWIIGYMCTFWWLMFFVYHYLPLTLPGFPAIDSPGAVLKRDGV 120

Query: 121 SGLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180
           SGLHPVVLVPGIVTGGLELWDGKPCA+GLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG
Sbjct: 121 SGLHPVVLVPGIVTGGLELWDGKPCAKGLFRKRLWGGSFTETLKRPLCWLEHLSLDNETG 180

Query: 181 LDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240
           LDPPGIR+RPVEGLVAADYF QGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV
Sbjct: 181 LDPPGIRIRPVEGLVAADYFTQGYFVWAVLIENLAKIGYDGKNLHMAAYDWRIAFQNTEV 240

Query: 241 RDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPMGGGGGGLGWCA 300
           RDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPM GGGGGLGWCA
Sbjct: 241 RDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHFMKWVEAPPPM-GGGGGLGWCA 300

Query: 301 KHIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTPGFLKSEILGFQTLEHLLRVSR 360
           K+IKAIMNIGSTFLGTPKAVSKILSAEDRH+ALLRAMTPGFLKSEILGFQTLEH+LRVSR
Sbjct: 301 KYIKAIMNIGSTFLGTPKAVSKILSAEDRHIALLRAMTPGFLKSEILGFQTLEHMLRVSR 360

Query: 361 TWDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKVDTHSFRNENDSNDSKRKKAFQEKEP 420
           TWDSTASLLPKGGETIWGDLDWSPEDVQNCDL+KVDTHSFRNENDSNDS+ KK  QEKE 
Sbjct: 361 TWDSTASLLPKGGETIWGDLDWSPEDVQNCDLEKVDTHSFRNENDSNDSEIKKGLQEKEA 420

Query: 421 VHYGRIISFSKEAATRPSSELSTLNSEELKNSGSSNDSSFSCSDVWTEYDEMNRESIRKV 480
           VHYGR+ISF KEAATRPSSELSTL SEEL +SGSSNDSSFSCSD WTEYDEM+RES+RKV
Sbjct: 421 VHYGRMISFGKEAATRPSSELSTLYSEELMDSGSSNDSSFSCSDGWTEYDEMSRESLRKV 480

Query: 481 SVNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDA 540
           S NKAYT KTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDA
Sbjct: 481 SENKAYTAKTVFDLLRVVAPKMMQRMDSHFSHGIAEDLDDPKYAHYKYWSNPLETKLPDA 540

Query: 541 PDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSIEGKGSGCLRSGMYFVDGDDSV 600
           PDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLS++G+GSGCLRSGMYFVDGDD V
Sbjct: 541 PDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRIDLSVDGEGSGCLRSGMYFVDGDDCV 600

Query: 601 PVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGVESSSHVDIMGNVNLI 660
           PVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRG+ESS+HVDIMGN  LI
Sbjct: 601 PVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHKAPASLLEGRGIESSAHVDIMGNAKLI 660

Query: 661 EDILRVAAGATGAEIGGDKIYSDILTLSERINLKL 695
           EDILRVAAGATGAEIGGD+IYSDILTLSERINLKL
Sbjct: 661 EDILRVAAGATGAEIGGDRIYSDILTLSERINLKL 694

BLAST of Lsi10G012050 vs. TAIR 10
Match: AT3G44830.1 (Lecithin:cholesterol acyltransferase family protein )

HSP 1 Score: 882.9 bits (2280), Expect = 1.7e-256
Identity = 433/662 (65.41%), Postives = 520/662 (78.55%), Query Frame = 0

Query: 43  KKLMQMKQD----KDKKKKRNKNIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLP 102
           +KL    +D    K K+    +  K+ +SG   C+D+CCW+IGY+CT WWL+ FLYH +P
Sbjct: 8   RKLSSFSEDTINPKPKQSATVEKPKRRRSGRCSCVDSCCWLIGYLCTAWWLLLFLYHSVP 67

Query: 103 LTLPGFRAIDSPGAVLKREGISGLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLWGGSF 162
           +     +A +SPG  L R+G+   HPV+LVPGIVTGGLELW+G+PCAEGLFRKRLWG SF
Sbjct: 68  VPAM-LQAPESPGTRLSRDGVKAFHPVILVPGIVTGGLELWEGRPCAEGLFRKRLWGASF 127

Query: 163 TETLKRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLAKIGY 222
           +E L+RPLCWLEHLSLD+ETGLDP GIRVR V GLVAADYFA  YF WAVLIENLAKIGY
Sbjct: 128 SEILRRPLCWLEHLSLDSETGLDPSGIRVRAVPGLVAADYFAPCYFAWAVLIENLAKIGY 187

Query: 223 DGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLYFLHF 282
           +GKNLHMA+YDWR++F NTEVRD++LSRLKSKIE+MY TNG  KVVVVPHSMG +YFLHF
Sbjct: 188 EGKNLHMASYDWRLSFHNTEVRDQSLSRLKSKIELMYATNGFKKVVVVPHSMGAIYFLHF 247

Query: 283 MKWVEAPPPMGGGGGGLGWCAKHIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLRAMTP 342
           +KWVE P P GGGGGG GWCAKHIK+++NIG  FLG PKAVS +LSAE + +A  R++ P
Sbjct: 248 LKWVETPLPDGGGGGGPGWCAKHIKSVVNIGPAFLGVPKAVSNLLSAEGKDIAYARSLAP 307

Query: 343 GFLKSEILGFQTLEHLLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNC--DLKKVDT 402
           G L SE+L  QTLEHL+R+S +WDS  SLLPKGGE IWGDLD   E+  NC    +K   
Sbjct: 308 GLLDSELLKLQTLEHLMRMSHSWDSIVSLLPKGGEAIWGDLDSHAEEGLNCIYSKRKSSQ 367

Query: 403 HSFRNENDSNDSKRKKAFQEKEPVHYGRIISFSKEAATRPSSELSTLNSEELKN-SGSSN 462
            S  N +  N S  K   + KEP  YGRI+SF K A+  PSS+LSTLN +EL    G+SN
Sbjct: 368 LSLSNLHKQNYS-LKPVSRVKEPAKYGRIVSFGKRASELPSSQLSTLNVKELSRVDGNSN 427

Query: 463 DSSFSCSDVWTEYDEMNRESIRKVSVNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIAE 522
           DS+ SC + W+EY+EM+RESI KV+ N AYT  TV DLLR +APKMM+R ++HFSHGIA+
Sbjct: 428 DST-SCGEFWSEYNEMSRESIVKVAENTAYTATTVLDLLRFIAPKMMRRAEAHFSHGIAD 487

Query: 523 DLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYD--SSYDKCK-SIPF 582
           DLDDPKY HYKYWSNPLETKLP+AP+ME+YCLYGVGIPTERSYIY   +S  KCK SIPF
Sbjct: 488 DLDDPKYGHYKYWSNPLETKLPEAPEMEMYCLYGVGIPTERSYIYKLATSSGKCKSSIPF 547

Query: 583 RIDLSIEGKGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRH 642
           RID S++G    CL+ G  F DGD+SVPV+S+GFMCAKGWR KTRFNPSG  T++REY+H
Sbjct: 548 RIDGSLDG-DDVCLKGGTRFADGDESVPVISAGFMCAKGWRGKTRFNPSGMDTFLREYKH 607

Query: 643 KAPASLLEGRGVESSSHVDIMGNVNLIEDILRVAAGATGAEIGGDKIYSDILTLSERINL 695
           K P SLLE RG ES +HVDIMGNV LIED+LR+AAGA+G EIGGD+IYSD++ +SERI++
Sbjct: 608 KPPGSLLESRGTESGAHVDIMGNVGLIEDVLRIAAGASGQEIGGDRIYSDVMRMSERISI 665

BLAST of Lsi10G012050 vs. TAIR 10
Match: AT5G13640.1 (phospholipid:diacylglycerol acyltransferase )

HSP 1 Score: 788.1 bits (2034), Expect = 5.7e-228
Identity = 383/661 (57.94%), Postives = 478/661 (72.31%), Query Frame = 0

Query: 47  QMKQDKDKKKK-----RNKNIKKGQSGEWKCLDNCCWIIGYMCTFWWLMFFLYHYLPLTL 106
           ++  D+D +KK     ++ + K    G+W C+D+CCW IG +C  WW + FLY+ +P + 
Sbjct: 20  EVVHDEDSQKKPHESSKSHHKKSNGGGKWSCIDSCCWFIGCVCVTWWFLLFLYNAMPASF 79

Query: 107 PGF-------RAIDSPGAVLKREGISGLHPVVLVPGIVTGGLELWDGKPCAEGLFRKRLW 166
           P +          D PG  LK+EG+   HPVV +PGIVTGGLELW+GK CA+GLFRKRLW
Sbjct: 80  PQYVTERITGPLPDPPGVKLKKEGLKAKHPVVFIPGIVTGGLELWEGKQCADGLFRKRLW 139

Query: 167 GGSFTETLKRPLCWLEHLSLDNETGLDPPGIRVRPVEGLVAADYFAQGYFVWAVLIENLA 226
           GG+F E  KRPLCW+EH+SLDNETGLDP GIRVR V GLVAADYFA GYFVWAVLI NLA
Sbjct: 140 GGTFGEVYKRPLCWVEHMSLDNETGLDPAGIRVRAVSGLVAADYFAPGYFVWAVLIANLA 199

Query: 227 KIGYDGKNLHMAAYDWRIAFQNTEVRDRALSRLKSKIEVMYETNGGNKVVVVPHSMGVLY 286
            IGY+ KN++MAAYDWR++FQNTEVRD+ LSR+KS IE+M  TNGG K V+VPHSMGVLY
Sbjct: 200 HIGYEEKNMYMAAYDWRLSFQNTEVRDQTLSRMKSNIELMVSTNGGKKAVIVPHSMGVLY 259

Query: 287 FLHFMKWVEAPPPMGGGGGGLGWCAKHIKAIMNIGSTFLGTPKAVSKILSAEDRHVALLR 346
           FLHFMKWVEAP P+GGGGG   WCAK+IKA+MNIG  FLG PKAV+ + SAE + VA+ R
Sbjct: 260 FLHFMKWVEAPAPLGGGGGP-DWCAKYIKAVMNIGGPFLGVPKAVAGLFSAEAKDVAVAR 319

Query: 347 AMTPGFLKSEILGFQTLEHLLRVSRTWDSTASLLPKGGETIWGDLDWSPEDVQNCDLKKV 406
           A+ PGFL ++I   QTL+H++R++RTWDST S+LPKGG+TIWG LDWSPE    C  KK 
Sbjct: 320 AIAPGFLDTDIFRLQTLQHVMRMTRTWDSTMSMLPKGGDTIWGGLDWSPEKGHTCCGKK- 379

Query: 407 DTHSFRNENDSNDSKRKKAFQEKEPVHYGRIISFSKEAATRPSSELSTLNSEELKNSGSS 466
                +  N++     +    +K PV+YGR+ISF KE A    SE++ ++        S 
Sbjct: 380 -----QKNNETCGEAGENGVSKKSPVNYGRMISFGKEVAEAAPSEINNIDFRGAVKGQSI 439

Query: 467 NDSSFSCSDVWTEYDEMNRESIRKVSVNKAYTVKTVFDLLRVVAPKMMQRMDSHFSHGIA 526
              + +C DVWTEY +M    I+ ++  K YT     DLL  VAPKMM R  +HFS+GIA
Sbjct: 440 --PNHTCRDVWTEYHDMGIAGIKAIAEYKVYTAGEAIDLLHYVAPKMMARGAAHFSYGIA 499

Query: 527 EDLDDPKYAHYKYWSNPLETKLPDAPDMEIYCLYGVGIPTERSYIYDSSYDKCKSIPFRI 586
           +DLDD KY   KYWSNPLETKLP+AP+MEIY LYGVGIPTER+Y+Y  +      IPF+I
Sbjct: 500 DDLDDTKYQDPKYWSNPLETKLPNAPEMEIYSLYGVGIPTERAYVYKLNQSPDSCIPFQI 559

Query: 587 DLSI-EGKGSGCLRSGMYFVDGDDSVPVVSSGFMCAKGWRNKTRFNPSGSPTYVREYRHK 646
             S  E     CL++G+Y VDGD++VPV+S+G+MCAK WR KTRFNPSG  TY+REY H 
Sbjct: 560 FTSAHEEDEDSCLKAGVYNVDGDETVPVLSAGYMCAKAWRGKTRFNPSGIKTYIREYNHS 619

Query: 647 APASLLEGRGVESSSHVDIMGNVNLIEDILRVAAGATGAEIGGDKIYSDILTLSERINLK 695
            PA+LLEGRG +S +HVDIMGN  LIEDI+RVAAG  G++IG D+++S I   SERI+LK
Sbjct: 620 PPANLLEGRGTQSGAHVDIMGNFALIEDIMRVAAGGNGSDIGHDQVHSGIFEWSERIDLK 671

BLAST of Lsi10G012050 vs. TAIR 10
Match: AT1G27480.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 46.6 bits (109), Expect = 9.5e-05
Identity = 60/248 (24.19%), Postives = 101/248 (40.73%), Query Frame = 0

Query: 120 SGLHPVVLVPGIVTGGLEL---WDGKP----CAEGLF---RK-----RLWGGSFTETLKR 179
           S ++P++LVPG     LE+    + KP    C+  L+   +K     RLW  +       
Sbjct: 29  SNVYPLILVPGNGGNQLEVRLDREYKPSSVWCSSWLYPIHKKSGGWFRLWFDAAVLLSPF 88

Query: 180 PLCWLEHLSLDNETGLD----PPGIRVR-PVEGLVAADYFAQGYFVWAV-LIENLA---- 239
             C+ + + L  +  LD     PG++ R P  G   +  +       A   +E+L     
Sbjct: 89  TRCFSDRMMLYYDPDLDDYQNAPGVQTRVPHFGSTKSLLYLDPRLRDATSYMEHLVKALE 148

Query: 240 -KIGY-DGKNLHMAAYDWRIAF----QNTEVRDRALSRLKSKIEVMYETNGGNKVVVVPH 299
            K GY + + +  A YD+R         + V  + L  LK  +E     N G  V+++ H
Sbjct: 149 KKCGYVNDQTILGAPYDFRYGLAASGHPSRVASQFLQDLKQLVEKTSSENEGKPVILLSH 208

Query: 300 SMGVLYFLHFMKWVEAPPPMGGGGGGLGWCAKHIKAIMNIGSTFLGTPKAVSKILSAEDR 337
           S+G L+ LHF+      P          W  K+IK  + + + + GT   +    S    
Sbjct: 209 SLGGLFVLHFLN--RTTP---------SWRRKYIKHFVALAAPWGGTISQMKTFASGNTL 265

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FYC72.4e-25565.41Putative phospholipid:diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana O... [more]
Q9FNA98.1e-22757.94Phospholipid:diacylglycerol acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN... [more]
P403452.5e-8232.21Phospholipid:diacylglycerol acyltransferase OS=Saccharomyces cerevisiae (strain ... [more]
O946803.2e-8233.84Phospholipid:diacylglycerol acyltransferase OS=Schizosaccharomyces pombe (strain... [more]
Q8WMP93.2e-1328.19Phospholipase A2 group XV OS=Bos taurus OX=9913 GN=PLA2G15 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7SR190.0e+0094.67Putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucumis melo var. maku... [more]
A0A1S3C9K80.0e+0094.67putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucumis melo OX=3656 G... [more]
A0A0A0KGM00.0e+0094.67Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G149960 PE=4 SV=1[more]
A0A6J1JAN30.0e+0092.81putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucurbita maxima OX=36... [more]
A0A6J1FW530.0e+0092.52putative phospholipid:diacylglycerol acyltransferase 2 OS=Cucurbita moschata OX=... [more]
Match NameE-valueIdentityDescription
XP_038902104.10.0e+0096.97putative phospholipid:diacylglycerol acyltransferase 2 [Benincasa hispida][more]
XP_008458650.10.0e+0094.67PREDICTED: putative phospholipid:diacylglycerol acyltransferase 2 [Cucumis melo][more]
KAA0033360.10.0e+0094.67putative phospholipid:diacylglycerol acyltransferase 2 [Cucumis melo var. makuwa... [more]
XP_004145827.10.0e+0094.67putative phospholipid:diacylglycerol acyltransferase 2 [Cucumis sativus] >KGN469... [more]
XP_022986276.10.0e+0092.81putative phospholipid:diacylglycerol acyltransferase 2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G44830.11.7e-25665.41Lecithin:cholesterol acyltransferase family protein [more]
AT5G13640.15.7e-22857.94phospholipid:diacylglycerol acyltransferase [more]
AT1G27480.19.5e-0524.19alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003386Lecithin:cholesterol/phospholipid:diacylglycerol acyltransferasePFAMPF02450LCATcoord: 149..654
e-value: 2.6E-67
score: 227.7
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 175..339
e-value: 2.4E-29
score: 104.5
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 123..325
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 398..417
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 436..460
NoneNo IPR availablePANTHERPTHR11440:SF87PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE 2-RELATEDcoord: 48..694
NoneNo IPR availablePANTHERPTHR11440LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATEDcoord: 48..694

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G012050.1Lsi10G012050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008374 O-acyltransferase activity