Lsi10G010300 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi10G010300
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Lipoxygenase
Location	chr10: 14578544 .. 14579023 (+)
RNA-Seq Expression	Lsi10G010300
Synteny	Lsi10G010300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGGTTACTTCTGGGCACCATGCTGCAGTTAACTTTGGACAATACCCTTTTTCGGCTAGTTATTATCCAACTCGACTTAGTCTTATGAGACTCAACATCCCCACAGAACAACCTAACTCACAACTTTGGAAGTGCTTTCTTGAAAATCTAGAAAATGTGTTTTTAAACACGTTTCCTACGCATTTTCAAACAATTGTACTTTTGTTCATTTTGAAGATATTGTCGAGTCATTCTCCTGAAGAGGAGTATTTGGGAGAGGATATGAAAGCAACATGGGCAGATGAAGGTGTTATAAAGGAAGCATTTATGAAGTTTAGAGCAAAATTGCAAAAATTAGAACAGATTATTGATAAAAGAAATGCCAATACTAAATTGAACAATAGACATGGAGCTGGGGTTAGGCCTTATCAACTTTTGAAGCCGTATTCAGAGCCTGGTGTAACGGCAAGAGGGGTTCCTAATAGTATTTCCATTTAA

mRNA sequence

ATGTGGGTTACTTCTGGGCACCATGCTGCAGTTAACTTTGGACAATACCCTTTTTCGGCTAGTTATTATCCAACTCGACTTAGTCTTATGAGACTCAACATCCCCACAGAACAACCTAACTCACAACTTTGGAAGTGCTTTCTTGAAAATCTAGAAAATGTGTTTTTAAACACGTTTCCTACGCATTTTCAAACAATTGTACTTTTGTTCATTTTGAAGATATTGTCGAGTCATTCTCCTGAAGAGGAGTATTTGGGAGAGGATATGAAAGCAACATGGGCAGATGAAGGTGTTATAAAGGAAGCATTTATGAAGTTTAGAGCAAAATTGCAAAAATTAGAACAGATTATTGATAAAAGAAATGCCAATACTAAATTGAACAATAGACATGGAGCTGGGGTTAGGCCTTATCAACTTTTGAAGCCGTATTCAGAGCCTGGTGTAACGGCAAGAGGGGTTCCTAATAGTATTTCCATTTAA

Coding sequence (CDS)

ATGTGGGTTACTTCTGGGCACCATGCTGCAGTTAACTTTGGACAATACCCTTTTTCGGCTAGTTATTATCCAACTCGACTTAGTCTTATGAGACTCAACATCCCCACAGAACAACCTAACTCACAACTTTGGAAGTGCTTTCTTGAAAATCTAGAAAATGTGTTTTTAAACACGTTTCCTACGCATTTTCAAACAATTGTACTTTTGTTCATTTTGAAGATATTGTCGAGTCATTCTCCTGAAGAGGAGTATTTGGGAGAGGATATGAAAGCAACATGGGCAGATGAAGGTGTTATAAAGGAAGCATTTATGAAGTTTAGAGCAAAATTGCAAAAATTAGAACAGATTATTGATAAAAGAAATGCCAATACTAAATTGAACAATAGACATGGAGCTGGGGTTAGGCCTTATCAACTTTTGAAGCCGTATTCAGAGCCTGGTGTAACGGCAAGAGGGGTTCCTAATAGTATTTCCATTTAA

Protein sequence

MWVTSGHHAAVNFGQYPFSASYYPTRLSLMRLNIPTEQPNSQLWKCFLENLENVFLNTFPTHFQTIVLLFILKILSSHSPEEEYLGEDMKATWADEGVIKEAFMKFRAKLQKLEQIIDKRNANTKLNNRHGAGVRPYQLLKPYSEPGVTARGVPNSISI

Homology

[Close]

BLAST of Lsi10G010300 vs. ExPASy Swiss-Prot
Match: O24370 (Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 2.7e-45
Identity = 87/159 (54.72%), Postives = 116/159 (72.96%), Query Frame = 0


Query: 1   MWVTSGHHAAVNFGQYPFSASYYPTRLSLMRLNIPTEQPNSQLWKCFLENLENVFLNTFP 60
           +WVTSGHHAAVNFGQY + A Y+P R ++ R  +PTE P ++ W+ F+   E   L  FP
Sbjct: 742 VWVTSGHHAAVNFGQYSY-AGYFPNRPTVARSKMPTEDPTAEEWEWFMNKPEEALLRCFP 801

Query: 61  THFQTIVLLFILKILSSHSPEEEYLGEDMKATWADEGVIKEAFMKFRAKLQKLEQIIDKR 120
           +  Q   ++ IL +LS+HSP+EEY+GE ++  WA++ VI  AF  F  KL++LE IID R
Sbjct: 802 SQIQATKVMAILDVLSNHSPDEEYIGEKIEPYWAEDPVINAAFEVFSGKLKELEGIIDAR 861

Query: 121 NANTKLNNRHGAGVRPYQLLKPYSEPGVTARGVPNSISI 160
           N ++KL+NR+GAGV PY+LLKPYSEPGVT +GVP SISI
Sbjct: 862 NNDSKLSNRNGAGVMPYELLKPYSEPGVTGKGVPYSISI 899

[Close]

BLAST of Lsi10G010300 vs. ExPASy Swiss-Prot
Match: R9WS04 (Lipoxygenase 2, chloroplastic OS=Tanacetum cinerariifolium OX=118510 GN=LOX2 PE=2 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 1.3e-42
Identity = 85/159 (53.46%), Postives = 110/159 (69.18%), Query Frame = 0


Query: 1   MWVTSGHHAAVNFGQYPFSASYYPTRLSLMRLNIPTEQPNSQLWKCFLENLENVFLNTFP 60
           MWV+SGHH+AVNFGQY F   Y+P R ++ R  +P E P  + W+ F+E  E+V LN FP
Sbjct: 742 MWVSSGHHSAVNFGQYDF-GGYFPNRPTIARTKMPNEDPTFEEWEAFMEKPEDVLLNCFP 801

Query: 61  THFQTIVLLFILKILSSHSPEEEYLGEDMKATWADEGVIKEAFMKFRAKLQKLEQIIDKR 120
           T  Q   ++ IL +LSSHSP+EEY+G  M+A+W  E  IK AF +F  +L+KL+ IID R
Sbjct: 802 TQIQATKVMAILDVLSSHSPDEEYIGTSMEASWEAEPAIKSAFEEFCGRLKKLDDIIDSR 861

Query: 121 NANTKLNNRHGAGVRPYQLLKPYSEPGVTARGVPNSISI 160
           N +  L NR GAG+  YQLLKP+S  GVT +GVP SISI
Sbjct: 862 NRDPILRNRTGAGLVQYQLLKPFSGHGVTGKGVPYSISI 899

[Close]

BLAST of Lsi10G010300 vs. ExPASy Swiss-Prot
Match: P38418 (Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX2 PE=1 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 2.8e-42
Identity = 86/158 (54.43%), Postives = 110/158 (69.62%), Query Frame = 0


Query: 2   WVTSGHHAAVNFGQYPFSASYYPTRLSLMRLNIPTEQPNSQLWKCFLENLENVFLNTFPT 61
           WVTSGHHAAVNFGQY +   Y+P R +  R+ +PTE P  +  K F E+ E V L T+P+
Sbjct: 740 WVTSGHHAAVNFGQYGY-GGYFPNRPTTTRIRMPTEDPTDEALKEFYESPEKVLLKTYPS 799

Query: 62  HFQTIVLLFILKILSSHSPEEEYLGEDMKATWADEGVIKEAFMKFRAKLQKLEQIIDKRN 121
             Q  +++  L +LS+HSP+EEY+GE  +A+WA+E VI  AF +F+ KLQ LE +ID+RN
Sbjct: 800 QKQATLVMVTLDLLSTHSPDEEYIGEQQEASWANEPVINAAFERFKGKLQYLEGVIDERN 859

Query: 122 ANTKLNNRHGAGVRPYQLLKPYSEPGVTARGVPNSISI 160
            N  L NR GAGV  Y+LLKP SE GVT  GVP SISI
Sbjct: 860 VNITLKNRAGAGVVKYELLKPTSEHGVTGMGVPYSISI 896

[Close]

BLAST of Lsi10G010300 vs. ExPASy Swiss-Prot
Match: Q8GSM2 (Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare OX=4513 GN=LOX2.3 PE=1 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 8.2e-42
Identity = 85/159 (53.46%), Postives = 109/159 (68.55%), Query Frame = 0


Query: 1   MWVTSGHHAAVNFGQYPFSASYYPTRLSLMRLNIPTEQPNSQLWKCFLENLENVFLNTFP 60
           MWVTSGHHAAVNFGQY + A Y+P R +++R NIP E+      K F+   E V L + P
Sbjct: 739 MWVTSGHHAAVNFGQYHY-AGYFPNRPTVVRRNIPVEENRDDEMKKFMARPEEVLLQSLP 798

Query: 61  THFQTIVLLFILKILSSHSPEEEYLGEDMKATWADEGVIKEAFMKFRAKLQKLEQIIDKR 120
           +  Q I ++  L ILSSHSP+EEY+GE  +  W  E ++K AF KF  +L++ E  ID R
Sbjct: 799 SQMQAIKVMATLDILSSHSPDEEYMGEYAEPAWLAEPMVKAAFEKFSGRLKEAEGTIDMR 858

Query: 121 NANTKLNNRHGAGVRPYQLLKPYSEPGVTARGVPNSISI 160
           N N +  NR GAG+ PY+LLKP+SEPGVT RG+PNSISI
Sbjct: 859 NNNPENKNRCGAGIVPYELLKPFSEPGVTGRGIPNSISI 896

[Close]

BLAST of Lsi10G010300 vs. ExPASy Swiss-Prot
Match: O24371 (Linoleate 13S-lipoxygenase 3-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LOX3.1 PE=1 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 8.8e-36
Identity = 80/160 (50.00%), Postives = 104/160 (65.00%), Query Frame = 0


Query: 1   MWVTSGHHAAVNFGQYPFSASYYPTRLSLMRLNIPTEQPNSQLWKCFLENLENVFLNTFP 60
           +W+ S  HAA+NFGQYP+   Y P R  LMR  IP E  N   +  FL + +  F +  P
Sbjct: 758 IWLASAQHAALNFGQYPY-GGYVPNRPPLMRRLIPDE--NDPEYAVFLADPQKYFFSALP 817

Query: 61  THFQTIVLLFILKILSSHSPEEEYLGE-DMKATWADEGVIKEAFMKFRAKLQKLEQIIDK 120
           +  Q    + ++  LS+HSP+EEYLGE    +TW  +  I EAF KF A++ ++E+ ID+
Sbjct: 818 SLLQATKFMAVVDTLSTHSPDEEYLGERHQPSTWTGDAEIVEAFYKFSAEIGRIEKEIDE 877

Query: 121 RNANTKLNNRHGAGVRPYQLLKPYSEPGVTARGVPNSISI 160
           RNANTKL NR GAGV PY+LL P S PGVT RGVPNS+SI
Sbjct: 878 RNANTKLKNRCGAGVLPYELLAPSSGPGVTCRGVPNSVSI 914

[Close]

BLAST of Lsi10G010300 vs. ExPASy TrEMBL
Match: A0A5A7TIA3 (Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00220 PE=3 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 1.7e-66
Identity = 128/160 (80.00%), Postives = 138/160 (86.25%), Query Frame = 0


Query: 1   MWVTSGHHAAVNFGQYPFSASYYPTRLSLMRLNIPTEQPNSQLWKCFLENLENVFLNTFP 60
           +WVTSGHHAAVNFGQY F+ASYYP R SL RLNIPTEQPNSQLW CFLEN ENVFL TFP
Sbjct: 720 IWVTSGHHAAVNFGQYSFAASYYPARPSLTRLNIPTEQPNSQLWNCFLENPENVFLETFP 779

Query: 61  THFQTIVLLFILKILSSHSPEEEYLGEDMKATWADEGVIKEAFMKFRAKLQKLEQIIDKR 120
           +HFQ  +LL ILKILSSHSP+EEYLG DM+A W DE VIKEAF KFRAKLQ LE IID+R
Sbjct: 780 SHFQATLLLSILKILSSHSPDEEYLGGDMEAAWEDEDVIKEAFYKFRAKLQNLEVIIDER 839

Query: 121 NANTKLNNRHG-AGVRPYQLLKPYSEPGVTARGVPNSISI 160
           NAN  L NRHG AG+ PYQLLKP+SEPGVTARGVPNS+SI
Sbjct: 840 NANPNLKNRHGAAGIGPYQLLKPFSEPGVTARGVPNSVSI 879

[Close]

BLAST of Lsi10G010300 vs. ExPASy TrEMBL
Match: A0A1S4DXN0 (Lipoxygenase OS=Cucumis melo OX=3656 GN=LOC103491674 PE=3 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 1.7e-66
Identity = 128/160 (80.00%), Postives = 138/160 (86.25%), Query Frame = 0


Query: 1   MWVTSGHHAAVNFGQYPFSASYYPTRLSLMRLNIPTEQPNSQLWKCFLENLENVFLNTFP 60
           +WVTSGHHAAVNFGQY F+ASYYP R SL RLNIPTEQPNSQLW CFLEN ENVFL TFP
Sbjct: 521 IWVTSGHHAAVNFGQYSFAASYYPARPSLTRLNIPTEQPNSQLWNCFLENPENVFLETFP 580

Query: 61  THFQTIVLLFILKILSSHSPEEEYLGEDMKATWADEGVIKEAFMKFRAKLQKLEQIIDKR 120
           +HFQ  +LL ILKILSSHSP+EEYLG DM+A W DE VIKEAF KFRAKLQ LE IID+R
Sbjct: 581 SHFQATLLLSILKILSSHSPDEEYLGGDMEAAWEDEDVIKEAFYKFRAKLQNLEVIIDER 640

Query: 121 NANTKLNNRHG-AGVRPYQLLKPYSEPGVTARGVPNSISI 160
           NAN  L NRHG AG+ PYQLLKP+SEPGVTARGVPNS+SI
Sbjct: 641 NANPNLKNRHGAAGIGPYQLLKPFSEPGVTARGVPNSVSI 680

[Close]

BLAST of Lsi10G010300 vs. ExPASy TrEMBL
Match: A0A5D3DKA2 (Linoleate 13S-lipoxygenase 2-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold80346G00010 PE=4 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 8.5e-66
Identity = 127/160 (79.38%), Postives = 138/160 (86.25%), Query Frame = 0


Query: 1   MWVTSGHHAAVNFGQYPFSASYYPTRLSLMRLNIPTEQPNSQLWKCFLENLENVFLNTFP 60
           +WVTSGHHAAVNFGQY F+ASYYP R SL RLNIPTEQPNSQLW CFLEN ENVFL TFP
Sbjct: 107 IWVTSGHHAAVNFGQYSFAASYYPARPSLTRLNIPTEQPNSQLWNCFLENPENVFLETFP 166

Query: 61  THFQTIVLLFILKILSSHSPEEEYLGEDMKATWADEGVIKEAFMKFRAKLQKLEQIIDKR 120
           +HFQ  +LL ILKILSSHSP+EEYLG DM+A W DE VIKEAF KFRAKLQ LE IID+R
Sbjct: 167 SHFQATLLLSILKILSSHSPDEEYLGGDMEAAWGDEDVIKEAFYKFRAKLQNLEVIIDER 226

Query: 121 NANTKLNNRHG-AGVRPYQLLKPYSEPGVTARGVPNSISI 160
           NAN  L NR+G AG+ PYQLLKP+SEPGVTARGVPNS+SI
Sbjct: 227 NANPNLKNRNGAAGIGPYQLLKPFSEPGVTARGVPNSVSI 266

[Close]

BLAST of Lsi10G010300 vs. ExPASy TrEMBL
Match: A0A6J1K0X9 (Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111491398 PE=3 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 1.7e-61
Identity = 121/159 (76.10%), Postives = 133/159 (83.65%), Query Frame = 0


Query: 1   MWVTSGHHAAVNFGQYPFSASYYPTRLSLMRLNIPTEQPNSQLWKCFLENLENVFLNTFP 60
           +WVTSGHHAAVNFGQYPF+ SYYP R SL RLNIPTE+PNS LWK FLEN ENVFL+TFP
Sbjct: 722 IWVTSGHHAAVNFGQYPFT-SYYPIRPSLTRLNIPTEEPNSTLWKYFLENPENVFLDTFP 781

Query: 61  THFQTIVLLFILKILSSHSPEEEYLGEDMKATWADEGVIKEAFMKFRAKLQKLEQIIDKR 120
           T  Q  +LLFIL ILSSHS +EEYLG DM+  W DE VIKEAF KF  KL+KLE+IID+R
Sbjct: 782 TQIQANILLFILNILSSHSRDEEYLGADMEPAWGDEPVIKEAFEKFSRKLKKLEEIIDER 841

Query: 121 NANTKLNNRHGAGVRPYQLLKPYSEPGVTARGVPNSISI 160
           N N KL NRHGAGV PYQ+LKPYSEPGVTARGVP S+SI
Sbjct: 842 NGNPKLKNRHGAGVGPYQILKPYSEPGVTARGVPCSVSI 879

[Close]

BLAST of Lsi10G010300 vs. ExPASy TrEMBL
Match: A0A6J1H3S4 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111459733 PE=3 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 6.9e-60
Identity = 120/159 (75.47%), Postives = 132/159 (83.02%), Query Frame = 0


Query: 1   MWVTSGHHAAVNFGQYPFSASYYPTRLSLMRLNIPTEQPNSQLWKCFLENLENVFLNTFP 60
           +WVTSGHHAAVNFGQYPF+ SYYP R SL RLNIPTE+PNS L K FLEN ENVFL+TFP
Sbjct: 722 IWVTSGHHAAVNFGQYPFT-SYYPIRPSLTRLNIPTEEPNSTLRKYFLENPENVFLDTFP 781

Query: 61  THFQTIVLLFILKILSSHSPEEEYLGEDMKATWADEGVIKEAFMKFRAKLQKLEQIIDKR 120
           T  Q  +LLFIL ILSSHS +EEYLG DM+  W DE VIKEAF KF  KL+KLE+IID+R
Sbjct: 782 TQIQANILLFILNILSSHSRDEEYLGADMEPAWGDEPVIKEAFEKFSRKLKKLEEIIDER 841

Query: 121 NANTKLNNRHGAGVRPYQLLKPYSEPGVTARGVPNSISI 160
           N N KL NRHGAGV PYQ+LKPYSEPGVTARGVP S+SI
Sbjct: 842 NGNPKLKNRHGAGVGPYQILKPYSEPGVTARGVPCSVSI 879

[Close]

BLAST of Lsi10G010300 vs. NCBI nr
Match: XP_038900841.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 275.0 bits (702), Expect = 4.0e-70
Identity = 130/159 (81.76%), Postives = 142/159 (89.31%), Query Frame = 0


Query: 1   MWVTSGHHAAVNFGQYPFSASYYPTRLSLMRLNIPTEQPNSQLWKCFLENLENVFLNTFP 60
           MWVTSGHHAAVNFGQYPF+ASYYP R SL RLNIPTEQPNS+LWKCFLEN ENVFL TFP
Sbjct: 706 MWVTSGHHAAVNFGQYPFAASYYPCRPSLTRLNIPTEQPNSKLWKCFLENPENVFLETFP 765

Query: 61  THFQTIVLLFILKILSSHSPEEEYLGEDMKATWADEGVIKEAFMKFRAKLQKLEQIIDKR 120
           THFQ  + L ILKILSSHSP+EEYLGED+K  WAD+GVIKEAF KFRAKLQKLE+IID+R
Sbjct: 766 THFQATIFLSILKILSSHSPDEEYLGEDLKEAWADDGVIKEAFHKFRAKLQKLEEIIDER 825

Query: 121 NANTKLNNRHGAGVRPYQLLKPYSEPGVTARGVPNSISI 160
           N + KL NRHGAG+ PY+LLKPYSEPGVTARGVP S+SI
Sbjct: 826 NVDPKLKNRHGAGIGPYELLKPYSEPGVTARGVPCSVSI 864

[Close]

BLAST of Lsi10G010300 vs. NCBI nr
Match: XP_016900733.1 (PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis melo])

HSP 1 Score: 261.9 bits (668), Expect = 3.5e-66
Identity = 128/160 (80.00%), Postives = 138/160 (86.25%), Query Frame = 0


Query: 1   MWVTSGHHAAVNFGQYPFSASYYPTRLSLMRLNIPTEQPNSQLWKCFLENLENVFLNTFP 60
           +WVTSGHHAAVNFGQY F+ASYYP R SL RLNIPTEQPNSQLW CFLEN ENVFL TFP
Sbjct: 521 IWVTSGHHAAVNFGQYSFAASYYPARPSLTRLNIPTEQPNSQLWNCFLENPENVFLETFP 580

Query: 61  THFQTIVLLFILKILSSHSPEEEYLGEDMKATWADEGVIKEAFMKFRAKLQKLEQIIDKR 120
           +HFQ  +LL ILKILSSHSP+EEYLG DM+A W DE VIKEAF KFRAKLQ LE IID+R
Sbjct: 581 SHFQATLLLSILKILSSHSPDEEYLGGDMEAAWEDEDVIKEAFYKFRAKLQNLEVIIDER 640

Query: 121 NANTKLNNRHG-AGVRPYQLLKPYSEPGVTARGVPNSISI 160
           NAN  L NRHG AG+ PYQLLKP+SEPGVTARGVPNS+SI
Sbjct: 641 NANPNLKNRHGAAGIGPYQLLKPFSEPGVTARGVPNSVSI 680

[Close]

BLAST of Lsi10G010300 vs. NCBI nr
Match: KAA0041377.1 (linoleate 13S-lipoxygenase 2-1 [Cucumis melo var. makuwa])

HSP 1 Score: 261.9 bits (668), Expect = 3.5e-66
Identity = 128/160 (80.00%), Postives = 138/160 (86.25%), Query Frame = 0


Query: 1   MWVTSGHHAAVNFGQYPFSASYYPTRLSLMRLNIPTEQPNSQLWKCFLENLENVFLNTFP 60
           +WVTSGHHAAVNFGQY F+ASYYP R SL RLNIPTEQPNSQLW CFLEN ENVFL TFP
Sbjct: 720 IWVTSGHHAAVNFGQYSFAASYYPARPSLTRLNIPTEQPNSQLWNCFLENPENVFLETFP 779

Query: 61  THFQTIVLLFILKILSSHSPEEEYLGEDMKATWADEGVIKEAFMKFRAKLQKLEQIIDKR 120
           +HFQ  +LL ILKILSSHSP+EEYLG DM+A W DE VIKEAF KFRAKLQ LE IID+R
Sbjct: 780 SHFQATLLLSILKILSSHSPDEEYLGGDMEAAWEDEDVIKEAFYKFRAKLQNLEVIIDER 839

Query: 121 NANTKLNNRHG-AGVRPYQLLKPYSEPGVTARGVPNSISI 160
           NAN  L NRHG AG+ PYQLLKP+SEPGVTARGVPNS+SI
Sbjct: 840 NANPNLKNRHGAAGIGPYQLLKPFSEPGVTARGVPNSVSI 879

[Close]

BLAST of Lsi10G010300 vs. NCBI nr
Match: XP_004142235.2 (linoleate 13S-lipoxygenase 2-1, chloroplastic isoform X1 [Cucumis sativus] >KAE8649499.1 hypothetical protein Csa_018002 [Cucumis sativus])

HSP 1 Score: 261.2 bits (666), Expect = 6.0e-66
Identity = 128/160 (80.00%), Postives = 137/160 (85.62%), Query Frame = 0


Query: 1   MWVTSGHHAAVNFGQYPFSASYYPTRLSLMRLNIPTEQPNSQLWKCFLENLENVFLNTFP 60
           +WVTSGHHAAVNFGQY F+ASYYP R SL RLNIPTEQPNSQLW CFLEN ENVFL TFP
Sbjct: 722 IWVTSGHHAAVNFGQYSFAASYYPARPSLTRLNIPTEQPNSQLWDCFLENPENVFLETFP 781

Query: 61  THFQTIVLLFILKILSSHSPEEEYLGEDMKATWADEGVIKEAFMKFRAKLQKLEQIIDKR 120
           THFQ  +LL ILKILS HSP+EEYLG DM+A W DE VIKEAF KFRAKLQ LE IID+R
Sbjct: 782 THFQATLLLSILKILSCHSPDEEYLGGDMEAAWEDEDVIKEAFYKFRAKLQNLEVIIDER 841

Query: 121 NANTKLNNRHG-AGVRPYQLLKPYSEPGVTARGVPNSISI 160
           NAN  L NRHG AG+ PYQLLKP+SEPGVTARGVPNS+SI
Sbjct: 842 NANPNLRNRHGAAGIGPYQLLKPFSEPGVTARGVPNSVSI 881

[Close]

BLAST of Lsi10G010300 vs. NCBI nr
Match: XP_031739579.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 261.2 bits (666), Expect = 6.0e-66
Identity = 128/160 (80.00%), Postives = 137/160 (85.62%), Query Frame = 0


Query: 1   MWVTSGHHAAVNFGQYPFSASYYPTRLSLMRLNIPTEQPNSQLWKCFLENLENVFLNTFP 60
           +WVTSGHHAAVNFGQY F+ASYYP R SL RLNIPTEQPNSQLW CFLEN ENVFL TFP
Sbjct: 720 IWVTSGHHAAVNFGQYSFAASYYPARPSLTRLNIPTEQPNSQLWDCFLENPENVFLETFP 779

Query: 61  THFQTIVLLFILKILSSHSPEEEYLGEDMKATWADEGVIKEAFMKFRAKLQKLEQIIDKR 120
           THFQ  +LL ILKILS HSP+EEYLG DM+A W DE VIKEAF KFRAKLQ LE IID+R
Sbjct: 780 THFQATLLLSILKILSCHSPDEEYLGGDMEAAWEDEDVIKEAFYKFRAKLQNLEVIIDER 839

Query: 121 NANTKLNNRHG-AGVRPYQLLKPYSEPGVTARGVPNSISI 160
           NAN  L NRHG AG+ PYQLLKP+SEPGVTARGVPNS+SI
Sbjct: 840 NANPNLRNRHGAAGIGPYQLLKPFSEPGVTARGVPNSVSI 879

[Close]

BLAST of Lsi10G010300 vs. TAIR 10
Match: AT3G45140.1 (lipoxygenase 2 )

HSP 1 Score: 172.9 bits (437), Expect = 2.0e-43
Identity = 86/158 (54.43%), Postives = 110/158 (69.62%), Query Frame = 0


Query: 2   WVTSGHHAAVNFGQYPFSASYYPTRLSLMRLNIPTEQPNSQLWKCFLENLENVFLNTFPT 61
           WVTSGHHAAVNFGQY +   Y+P R +  R+ +PTE P  +  K F E+ E V L T+P+
Sbjct: 740 WVTSGHHAAVNFGQYGY-GGYFPNRPTTTRIRMPTEDPTDEALKEFYESPEKVLLKTYPS 799

Query: 62  HFQTIVLLFILKILSSHSPEEEYLGEDMKATWADEGVIKEAFMKFRAKLQKLEQIIDKRN 121
             Q  +++  L +LS+HSP+EEY+GE  +A+WA+E VI  AF +F+ KLQ LE +ID+RN
Sbjct: 800 QKQATLVMVTLDLLSTHSPDEEYIGEQQEASWANEPVINAAFERFKGKLQYLEGVIDERN 859

Query: 122 ANTKLNNRHGAGVRPYQLLKPYSEPGVTARGVPNSISI 160
            N  L NR GAGV  Y+LLKP SE GVT  GVP SISI
Sbjct: 860 VNITLKNRAGAGVVKYELLKPTSEHGVTGMGVPYSISI 896

[Close]

BLAST of Lsi10G010300 vs. TAIR 10
Match: AT1G72520.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 139.4 bits (350), Expect = 2.5e-33
Identity = 72/160 (45.00%), Postives = 102/160 (63.75%), Query Frame = 0


Query: 1   MWVTSGHHAAVNFGQYPFSASYYPTRLSLMRLNIPTEQPNSQLWKCFLENLENVFLNTFP 60
           +W+ S  HAA+NFGQYP+   Y P R  LMR  IP E  +   +  F+E+ +  F ++ P
Sbjct: 770 IWLASAQHAALNFGQYPY-GGYVPNRPPLMRRLIPDE--SDPEFTSFIEDPQKYFFSSMP 829

Query: 61  THFQTIVLLFILKILSSHSPEEEYLGE-DMKATWADEGVIKEAFMKFRAKLQKLEQIIDK 120
           +  QT   + ++  LS+HSP+EEY+GE    + W  +  I +AF  F A++ ++E+ IDK
Sbjct: 830 SLLQTTKFMAVVDTLSTHSPDEEYIGERQQPSIWTGDAEIVDAFYGFSAEIGRIEKEIDK 889

Query: 121 RNANTKLNNRHGAGVRPYQLLKPYSEPGVTARGVPNSISI 160
           RN +    NR GAGV PY+L+ P SEPGVT RGVPNS+SI
Sbjct: 890 RNRDPSRRNRCGAGVLPYELMAPSSEPGVTCRGVPNSVSI 926

[Close]

BLAST of Lsi10G010300 vs. TAIR 10
Match: AT1G17420.1 (lipoxygenase 3 )

HSP 1 Score: 138.3 bits (347), Expect = 5.5e-33
Identity = 73/160 (45.62%), Postives = 102/160 (63.75%), Query Frame = 0


Query: 1   MWVTSGHHAAVNFGQYPFSASYYPTRLSLMRLNIPTEQPNSQLWKCFLENLENVFLNTFP 60
           +W+ S  HAA+NFGQYP+   Y P R  LMR  IP E  +   +  F+ + E  + ++ P
Sbjct: 763 IWLASAQHAALNFGQYPY-GGYVPNRPPLMRRLIPDE--SDPEYASFISHPEKYYFSSMP 822

Query: 61  THFQTIVLLFILKILSSHSPEEEYLGE-DMKATWADEGVIKEAFMKFRAKLQKLEQIIDK 120
           +  QT   + ++  LS+HSP+EEY+GE    + W  +  I EAF  F A++ ++E+ I+K
Sbjct: 823 SLAQTSKFMAVVDTLSTHSPDEEYIGERQQPSIWTGDAEIVEAFYGFAAEIGRIEKEIEK 882

Query: 121 RNANTKLNNRHGAGVRPYQLLKPYSEPGVTARGVPNSISI 160
           RNA+    NR GAGV PY+LL P SEPGVT RGVPNS+SI
Sbjct: 883 RNADPDRRNRCGAGVLPYELLVPSSEPGVTCRGVPNSVSI 919

[Close]

BLAST of Lsi10G010300 vs. TAIR 10
Match: AT1G67560.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 136.3 bits (342), Expect = 2.1e-32
Identity = 72/161 (44.72%), Postives = 103/161 (63.98%), Query Frame = 0


Query: 1   MWVTSGHHAAVNFGQYPFSASYYPTRLSLMRLNIPTEQPNSQLWKCFLENLENVFLNTFP 60
           +W+ SG HAA+NFGQYPF   Y P R +L+R  IP E      ++ F+ N +  FL + P
Sbjct: 760 IWIASGQHAAINFGQYPF-GGYVPNRPTLLRKLIPQETDPD--YEMFMRNPQYSFLGSLP 819

Query: 61  THFQTIVLLFILKILSSHSPEEEYLGE--DMKATWADEGVIKEAFMKFRAKLQKLEQIID 120
           T  Q   ++ + + LS+HSP+EEYL E  +++  W  +  + + F KF  +L K+E+ I+
Sbjct: 820 TQLQATKVMAVQETLSTHSPDEEYLIELREVQRHWFQDEQVVKYFNKFSEELVKIEKTIN 879

Query: 121 KRNANTKLNNRHGAGVRPYQLLKPYSEPGVTARGVPNSISI 160
           +RN + KL NR GAG+ PY+LL P S  GVT RG+PNSISI
Sbjct: 880 ERNKDKKLKNRTGAGMPPYELLLPTSPHGVTGRGIPNSISI 917

[Close]

BLAST of Lsi10G010300 vs. TAIR 10
Match: AT1G55020.1 (lipoxygenase 1 )

HSP 1 Score: 128.3 bits (321), Expect = 5.7e-30
Identity = 72/159 (45.28%), Postives = 99/159 (62.26%), Query Frame = 0


Query: 1   MWVTSGHHAAVNFGQYPFSASYYPTRLSLMRLNIPTEQPNSQLWKCFLENLENVFLNTFP 60
           +WV S  HAAVNFGQYP  A Y P R ++ R  +P E  N+  ++   +N + VFL T  
Sbjct: 704 IWVASALHAAVNFGQYPV-AGYLPNRPTISRQYMPKE--NTPEFEELEKNPDKVFLKTIT 763

Query: 61  THFQTIVLLFILKILSSHSPEEEYLGEDMKATWADEGVIKEAFMKFRAKLQKLEQIIDKR 120
              QT++ + +++ILS+HS +E YLG+     WA E    EAF KF  K++++E+ ID+R
Sbjct: 764 AQLQTLLGISLIEILSTHSSDEVYLGQRDSKEWAAEKEALEAFEKFGEKVKEIEKNIDER 823

Query: 121 NANTKLNNRHGAGVRPYQLLKPYSEPGVTARGVPNSISI 160
           N +  L NR G    PY LL P SE GVT RG+PNS+SI
Sbjct: 824 NDDETLKNRTGLVKMPYTLLFPSSEGGVTGRGIPNSVSI 859

The following BLAST results are available for this feature:

BLAST of Lsi10G010300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24370	2.7e-45	54.72	Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LO...	[more]
R9WS04	1.3e-42	53.46	Lipoxygenase 2, chloroplastic OS=Tanacetum cinerariifolium OX=118510 GN=LOX2 PE=...	[more]
P38418	2.8e-42	54.43	Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX2 PE=1 SV=1	[more]
Q8GSM2	8.2e-42	53.46	Lipoxygenase 2.3, chloroplastic OS=Hordeum vulgare OX=4513 GN=LOX2.3 PE=1 SV=1	[more]
O24371	8.8e-36	50.00	Linoleate 13S-lipoxygenase 3-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LO...	[more]

BLAST of Lsi10G010300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TIA3	1.7e-66	80.00	Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00220 P...	[more]
A0A1S4DXN0	1.7e-66	80.00	Lipoxygenase OS=Cucumis melo OX=3656 GN=LOC103491674 PE=3 SV=1	[more]
A0A5D3DKA2	8.5e-66	79.38	Linoleate 13S-lipoxygenase 2-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A6J1K0X9	1.7e-61	76.10	Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111491398 PE=3 SV=1	[more]
A0A6J1H3S4	6.9e-60	75.47	Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111459733 PE=3 SV=1	[more]

BLAST of Lsi10G010300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038900841.1	4.0e-70	81.76	linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Benincasa hispida]	[more]
XP_016900733.1	3.5e-66	80.00	PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis melo]	[more]
KAA0041377.1	3.5e-66	80.00	linoleate 13S-lipoxygenase 2-1 [Cucumis melo var. makuwa]	[more]
XP_004142235.2	6.0e-66	80.00	linoleate 13S-lipoxygenase 2-1, chloroplastic isoform X1 [Cucumis sativus] >KAE8...	[more]
XP_031739579.1	6.0e-66	80.00	linoleate 13S-lipoxygenase 2-1, chloroplastic isoform X2 [Cucumis sativus]	[more]

BLAST of Lsi10G010300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G45140.1	2.0e-43	54.43	lipoxygenase 2	[more]
AT1G72520.1	2.5e-33	45.00	PLAT/LH2 domain-containing lipoxygenase family protein	[more]
AT1G17420.1	5.5e-33	45.63	lipoxygenase 3	[more]
AT1G67560.1	2.1e-32	44.72	PLAT/LH2 domain-containing lipoxygenase family protein	[more]
AT1G55020.1	5.7e-30	45.28	lipoxygenase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013819	Lipoxygenase, C-terminal	PFAM	PF00305	Lipoxygenase	coord: 1..142 e-value: 8.0E-47 score: 160.1
IPR013819	Lipoxygenase, C-terminal	PROSITE	PS51393	LIPOXYGENASE_3	coord: 1..159 score: 50.358273
None	No IPR available	GENE3D	1.20.245.10		coord: 1..158 e-value: 4.3E-40 score: 139.9
None	No IPR available	PANTHER	PTHR11771:SF89	LINOLEATE 13S-LIPOXYGENASE 2-1, CHLOROPLASTIC	coord: 1..158
IPR000907	Lipoxygenase	PANTHER	PTHR11771	LIPOXYGENASE	coord: 1..158
IPR036226	Lipoxigenase, C-terminal domain superfamily	SUPERFAMILY	48484	Lipoxigenase	coord: 1..159

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G010300.1	Lsi10G010300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006633	fatty acid biosynthetic process
biological_process	GO:0031408	oxylipin biosynthetic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016702	oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen