Lsi10G010250 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi10G010250
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Lipoxygenase
Location	chr10: 14538168 .. 14538575 (+)
RNA-Seq Expression	Lsi10G010250
Synteny	Lsi10G010250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGAAATCTCAAGGCTTTAGATCTTTCACAGGGGTGGCTCCGGCAACCGGCCTCTGCCTTCTGCAGAGGCCGTTCGGTCGAAGCATCAACCGCGGTACTTTGGTTCCTAATGGAAATAATAATAATAATCTCCTGGAGAAGGAGATTAAGATCAAGAATAATAATGGAGGGCTGAGATTAGTGTCCGGACGTGGAATAATCAAAGCAGCCTTAGCCACAGCCACGGAACCCATCACCAGTGTCATCACAAGAGTCATTGTGAAGATCACAGGAGAAATCACTTCATCTACAATTGTCAAAGAAAACCAAGCCCCTCAAAAGCTGCTCAAGCTTGAGTTTGCCAGCATCCTCCTCGATCCTAGTAATGTTCTCTTCATATACCGATTATTTAAGAGAAATGATTAG

mRNA sequence

ATGTCGAAATCTCAAGGCTTTAGATCTTTCACAGGGGTGGCTCCGGCAACCGGCCTCTGCCTTCTGCAGAGGCCGTTCGGTCGAAGCATCAACCGCGGTACTTTGGTTCCTAATGGAAATAATAATAATAATCTCCTGGAGAAGGAGATTAAGATCAAGAATAATAATGGAGGGCTGAGATTAGTGTCCGGACGTGGAATAATCAAAGCAGCCTTAGCCACAGCCACGGAACCCATCACCAGTGTCATCACAAGAGTCATTGTGAAGATCACAGGAGAAATCACTTCATCTACAATTGTCAAAGAAAACCAAGCCCCTCAAAAGCTGCTCAAGCTTGAGTTTGCCAGCATCCTCCTCGATCCTAGTAATGTTCTCTTCATATACCGATTATTTAAGAGAAATGATTAG

Coding sequence (CDS)

ATGTCGAAATCTCAAGGCTTTAGATCTTTCACAGGGGTGGCTCCGGCAACCGGCCTCTGCCTTCTGCAGAGGCCGTTCGGTCGAAGCATCAACCGCGGTACTTTGGTTCCTAATGGAAATAATAATAATAATCTCCTGGAGAAGGAGATTAAGATCAAGAATAATAATGGAGGGCTGAGATTAGTGTCCGGACGTGGAATAATCAAAGCAGCCTTAGCCACAGCCACGGAACCCATCACCAGTGTCATCACAAGAGTCATTGTGAAGATCACAGGAGAAATCACTTCATCTACAATTGTCAAAGAAAACCAAGCCCCTCAAAAGCTGCTCAAGCTTGAGTTTGCCAGCATCCTCCTCGATCCTAGTAATGTTCTCTTCATATACCGATTATTTAAGAGAAATGATTAG

Protein sequence

MSKSQGFRSFTGVAPATGLCLLQRPFGRSINRGTLVPNGNNNNNLLEKEIKIKNNNGGLRLVSGRGIIKAALATATEPITSVITRVIVKITGEITSSTIVKENQAPQKLLKLEFASILLDPSNVLFIYRLFKRND

Homology

[Close]

BLAST of Lsi10G010250 vs. ExPASy TrEMBL
Match: A0A0A0L0L3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G288080 PE=3 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 1.6e-20
Identity = 81/130 (62.31%), Postives = 88/130 (67.69%), Query Frame = 0


Query: 1   MSKSQGFRSFTGVAPATGLCLLQRPFGRSINRGTLVPNGNNNNNLLEKE---IKIKNN-- 60
           M KSQG  S  GVAP +          R I+   LVP  NNN+  L+K+   IKIKNN  
Sbjct: 1   MLKSQGLGSSIGVAPVSLHHTRTSLARRGIDGSALVPYQNNND--LQKKLRGIKIKNNND 60

Query: 61  NGGLRLVSGRGIIKAALATATEPITSVITRVIV-KITGEITSSTIVKENQ--APQKLLKL 120
           NGGLR +SG GIIKAALATATE  TSVIT V V KITGEITSSTIVKENQ  AP K L+L
Sbjct: 61  NGGLRSLSGSGIIKAALATATETTTSVITTVTVKKITGEITSSTIVKENQLAAPPKFLQL 120

Query: 121 EFASILLDPS 123
            FASIL DPS
Sbjct: 121 GFASILTDPS 128

[Close]

BLAST of Lsi10G010250 vs. ExPASy TrEMBL
Match: A0A5D3DDW6 (Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00020 PE=3 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 2.0e-20
Identity = 83/132 (62.88%), Postives = 95/132 (71.97%), Query Frame = 0


Query: 1   MSKSQGFRSFTGVAPATGLCLLQRPFG--RSINRGTLVPNGNNNNNLLEKE---IKIKN- 60
           M KSQG  S  GVAP T L  +Q      R+I+   LVP  NNN+  L+K+   IKIKN 
Sbjct: 1   MLKSQGLGSSIGVAPVT-LRHMQTSLARRRNIDGSALVPYQNNND--LQKKLRGIKIKNN 60

Query: 61  -NNGGLRLVSG-RGIIKAALATATEPITSVITRVIV-KITGEITSSTIVKENQ-APQKLL 120
            NNGGLRL+SG  G IKAALATATE  TSVIT+V+V KITGEITSSTI+KENQ A QK+L
Sbjct: 61  DNNGGLRLMSGPSGTIKAALATATELTTSVITKVVVKKITGEITSSTIIKENQLATQKVL 120

Query: 121 KLEFASILLDPS 123
           +L FASIL DPS
Sbjct: 121 QLGFASILTDPS 129

[Close]

BLAST of Lsi10G010250 vs. ExPASy TrEMBL
Match: A0A5A7TH45 (Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00210 PE=3 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 2.0e-20
Identity = 83/132 (62.88%), Postives = 95/132 (71.97%), Query Frame = 0


Query: 1   MSKSQGFRSFTGVAPATGLCLLQRPFG--RSINRGTLVPNGNNNNNLLEKE---IKIKN- 60
           M KSQG  S  GVAP T L  +Q      R+I+   LVP  NNN+  L+K+   IKIKN 
Sbjct: 1   MLKSQGLGSSIGVAPVT-LRHMQTSLARRRNIDGSALVPYQNNND--LQKKLRGIKIKNN 60

Query: 61  -NNGGLRLVSG-RGIIKAALATATEPITSVITRVIV-KITGEITSSTIVKENQ-APQKLL 120
            NNGGLRL+SG  G IKAALATATE  TSVIT+V+V KITGEITSSTI+KENQ A QK+L
Sbjct: 61  DNNGGLRLMSGPSGTIKAALATATELTTSVITKVVVKKITGEITSSTIIKENQLATQKVL 120

Query: 121 KLEFASILLDPS 123
           +L FASIL DPS
Sbjct: 121 QLGFASILTDPS 129

[Close]

BLAST of Lsi10G010250 vs. ExPASy TrEMBL
Match: A0A1S3BMR0 (Lipoxygenase OS=Cucumis melo OX=3656 GN=LOC103491540 PE=3 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 2.0e-20
Identity = 83/132 (62.88%), Postives = 95/132 (71.97%), Query Frame = 0


Query: 1   MSKSQGFRSFTGVAPATGLCLLQRPFG--RSINRGTLVPNGNNNNNLLEKE---IKIKN- 60
           M KSQG  S  GVAP T L  +Q      R+I+   LVP  NNN+  L+K+   IKIKN 
Sbjct: 1   MLKSQGLGSSIGVAPVT-LRHMQTSLARRRNIDGSALVPYQNNND--LQKKLRGIKIKNN 60

Query: 61  -NNGGLRLVSG-RGIIKAALATATEPITSVITRVIV-KITGEITSSTIVKENQ-APQKLL 120
            NNGGLRL+SG  G IKAALATATE  TSVIT+V+V KITGEITSSTI+KENQ A QK+L
Sbjct: 61  DNNGGLRLMSGPSGTIKAALATATELTTSVITKVVVKKITGEITSSTIIKENQLATQKVL 120

Query: 121 KLEFASILLDPS 123
           +L FASIL DPS
Sbjct: 121 QLGFASILTDPS 129

[Close]

BLAST of Lsi10G010250 vs. ExPASy TrEMBL
Match: A0A6J1H2B7 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111459461 PE=3 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 3.3e-18
Identity = 74/127 (58.27%), Postives = 84/127 (66.14%), Query Frame = 0


Query: 1   MSKSQGFRSFTGVAPATGLCLLQRP-FGRSINRGTLVPNGNNNNNLLEKEIKIKNNNGGL 60
           M KSQG    T VAPAT LC LQRP  GR IN G   P  NN  N   K+     +NG L
Sbjct: 1   MLKSQGVGCSTRVAPAT-LCHLQRPSLGRGINAGVSFPFWNNPGN---KQKAKSISNGRL 60

Query: 61  RLVSGRGIIKAALATATEPITSVITRVIV-KITGEITSSTIVKENQAPQKLLKLEFASIL 120
           RLVS    IKAAL T  EP T+V+T+VIV KITGEITSST++ E++A  KLLKLEFASIL
Sbjct: 61  RLVSRE--IKAALTTVAEPTTTVLTKVIVKKITGEITSSTLLMESKALDKLLKLEFASIL 120

Query: 121 LDPSNVL 126
           +DP   L
Sbjct: 121 VDPKTGL 121

[Close]

BLAST of Lsi10G010250 vs. NCBI nr
Match: XP_038901038.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 145.6 bits (366), Expect = 3.1e-31
Identity = 94/124 (75.81%), Postives = 100/124 (80.65%), Query Frame = 0


Query: 1   MSKSQGFRSFTGVAPATGLCLLQRPFGRSINRGTLVPNGNNNNNLLEKEIK-IK-NNNGG 60
           M KSQGF S T VAPAT LC LQRP G SIN G LV   NNNN  LEK++K IK   NGG
Sbjct: 1   MLKSQGFGSSTRVAPAT-LCHLQRPLGHSINGGALVAYRNNNN--LEKQLKGIKIKKNGG 60

Query: 61  LRLVSGRGIIKAALATATEPITSVITRVIV-KITGEITSSTIVKENQAPQKLLKLEFASI 120
           LRLVSG GIIKAALATATEP T+VIT+VIV KITGEITSS I+KENQAPQKLL+L FASI
Sbjct: 61  LRLVSGGGIIKAALATATEPTTTVITKVIVKKITGEITSSKILKENQAPQKLLQLGFASI 120

Query: 121 LLDP 122
           LLDP
Sbjct: 121 LLDP 121

[Close]

BLAST of Lsi10G010250 vs. NCBI nr
Match: XP_004142137.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic [Cucumis sativus] >KGN54488.2 hypothetical protein Csa_018102 [Cucumis sativus])

HSP 1 Score: 109.0 bits (271), Expect = 3.2e-20
Identity = 81/130 (62.31%), Postives = 88/130 (67.69%), Query Frame = 0


Query: 1   MSKSQGFRSFTGVAPATGLCLLQRPFGRSINRGTLVPNGNNNNNLLEKE---IKIKNN-- 60
           M KSQG  S  GVAP +          R I+   LVP  NNN+  L+K+   IKIKNN  
Sbjct: 1   MLKSQGLGSSIGVAPVSLHHTRTSLARRGIDGSALVPYQNNND--LQKKLRGIKIKNNND 60

Query: 61  NGGLRLVSGRGIIKAALATATEPITSVITRVIV-KITGEITSSTIVKENQ--APQKLLKL 120
           NGGLR +SG GIIKAALATATE  TSVIT V V KITGEITSSTIVKENQ  AP K L+L
Sbjct: 61  NGGLRSLSGSGIIKAALATATETTTSVITTVTVKKITGEITSSTIVKENQLAAPPKFLQL 120

Query: 121 EFASILLDPS 123
            FASIL DPS
Sbjct: 121 GFASILTDPS 128

[Close]

BLAST of Lsi10G010250 vs. NCBI nr
Match: KAA0041376.1 (linoleate 13S-lipoxygenase 2-1 [Cucumis melo var. makuwa])

HSP 1 Score: 108.6 bits (270), Expect = 4.2e-20
Identity = 83/132 (62.88%), Postives = 95/132 (71.97%), Query Frame = 0


Query: 1   MSKSQGFRSFTGVAPATGLCLLQRPFG--RSINRGTLVPNGNNNNNLLEKE---IKIKN- 60
           M KSQG  S  GVAP T L  +Q      R+I+   LVP  NNN+  L+K+   IKIKN 
Sbjct: 1   MLKSQGLGSSIGVAPVT-LRHMQTSLARRRNIDGSALVPYQNNND--LQKKLRGIKIKNN 60

Query: 61  -NNGGLRLVSG-RGIIKAALATATEPITSVITRVIV-KITGEITSSTIVKENQ-APQKLL 120
            NNGGLRL+SG  G IKAALATATE  TSVIT+V+V KITGEITSSTI+KENQ A QK+L
Sbjct: 61  DNNGGLRLMSGPSGTIKAALATATELTTSVITKVVVKKITGEITSSTIIKENQLATQKVL 120

Query: 121 KLEFASILLDPS 123
           +L FASIL DPS
Sbjct: 121 QLGFASILTDPS 129

[Close]

BLAST of Lsi10G010250 vs. NCBI nr
Match: XP_008449749.1 (PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis melo] >TYK21640.1 linoleate 13S-lipoxygenase 2-1 [Cucumis melo var. makuwa])

HSP 1 Score: 108.6 bits (270), Expect = 4.2e-20
Identity = 83/132 (62.88%), Postives = 95/132 (71.97%), Query Frame = 0


Query: 1   MSKSQGFRSFTGVAPATGLCLLQRPFG--RSINRGTLVPNGNNNNNLLEKE---IKIKN- 60
           M KSQG  S  GVAP T L  +Q      R+I+   LVP  NNN+  L+K+   IKIKN 
Sbjct: 1   MLKSQGLGSSIGVAPVT-LRHMQTSLARRRNIDGSALVPYQNNND--LQKKLRGIKIKNN 60

Query: 61  -NNGGLRLVSG-RGIIKAALATATEPITSVITRVIV-KITGEITSSTIVKENQ-APQKLL 120
            NNGGLRL+SG  G IKAALATATE  TSVIT+V+V KITGEITSSTI+KENQ A QK+L
Sbjct: 61  DNNGGLRLMSGPSGTIKAALATATELTTSVITKVVVKKITGEITSSTIIKENQLATQKVL 120

Query: 121 KLEFASILLDPS 123
           +L FASIL DPS
Sbjct: 121 QLGFASILTDPS 129

[Close]

BLAST of Lsi10G010250 vs. NCBI nr
Match: KAG6605891.1 (Linoleate 13S-lipoxygenase 2-1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 103.6 bits (257), Expect = 1.4e-18
Identity = 73/126 (57.94%), Postives = 83/126 (65.87%), Query Frame = 0


Query: 1   MSKSQGFRSFTGVAPATGLCLLQRPFGRSINRGTLVPNGNNNNNLLEKEIKIKNNNGGLR 60
           M KSQG    T VAPAT LC LQRP  R IN G   P  NN  N   K+     +NG LR
Sbjct: 1   MLKSQGVGCATRVAPAT-LCHLQRPLPRGINAGVSFPFWNNTQN---KQKAKSISNGRLR 60

Query: 61  LVSGRGIIKAALATATEPITSVITRVIV-KITGEITSSTIVKENQAPQKLLKLEFASILL 120
           LVS    IKAAL T  EP T+V+T+VIV KITGEITSST++ E++A  KLLKLEFASIL+
Sbjct: 61  LVSRE--IKAALTTVAEPTTTVLTKVIVKKITGEITSSTLLMESKALDKLLKLEFASILV 120

Query: 121 DPSNVL 126
           DP   L
Sbjct: 121 DPKTGL 120

The following BLAST results are available for this feature:

BLAST of Lsi10G010250 vs.


Match Name	E-value	Identity	Description

BLAST of Lsi10G010250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L0L3	1.6e-20	62.31	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G288080 PE=3 SV=1	[more]
A0A5D3DDW6	2.0e-20	62.88	Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00020 P...	[more]
A0A5A7TH45	2.0e-20	62.88	Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00210 P...	[more]
A0A1S3BMR0	2.0e-20	62.88	Lipoxygenase OS=Cucumis melo OX=3656 GN=LOC103491540 PE=3 SV=1	[more]
A0A6J1H2B7	3.3e-18	58.27	Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111459461 PE=3 SV=1	[more]

BLAST of Lsi10G010250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038901038.1	3.1e-31	75.81	linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Benincasa hispida]	[more]
XP_004142137.1	3.2e-20	62.31	linoleate 13S-lipoxygenase 2-1, chloroplastic [Cucumis sativus] >KGN54488.2 hypo...	[more]
KAA0041376.1	4.2e-20	62.88	linoleate 13S-lipoxygenase 2-1 [Cucumis melo var. makuwa]	[more]
XP_008449749.1	4.2e-20	62.88	PREDICTED: linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucumis melo] >TY...	[more]
KAG6605891.1	1.4e-18	57.94	Linoleate 13S-lipoxygenase 2-1, chloroplastic, partial [Cucurbita argyrosperma s...	[more]

BLAST of Lsi10G010250 vs. NCBI nr


Match Name	E-value	Identity	Description

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G010250.1	Lsi10G010250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046394	carboxylic acid biosynthetic process
biological_process	GO:0006631	fatty acid metabolic process
molecular_function	GO:0051213	dioxygenase activity