Lsi10G010210 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G010210
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description(1->3)-beta-glucan endohydrolase
Locationchr10: 14493952 .. 14495388 (-)
RNA-Seq ExpressionLsi10G010210
SyntenyLsi10G010210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTACCACTCTTCTTCGCTCTACTGTTTTCTTCTTCTTTAACTTTCTATTGGCCACTGACATCGTCTATGCGCAATCGTTCATCGGAATAAACTACGGTCAAGTCGCCGATAACCTTCCACCTCCGTCCGCGACGGCGAAGCTACTTCAATCCACTTCAATCCAGAAAGTTCGACTCTACGGCGCTGATCCGGCCATCATCAAAGCCCTAGCAAACACAGGGATCGGAATTGTAATCGGCGCTGCGAACGGTGACATTCCAGCCCTAGCGTCCGACCCGAATTTCGCCAAAAATTGGGTTAACGCCAATGTAGCCCCCTTCCATCCAGCGAGTAAAATCATTCTCATCACGGTCGGCAACGAGGTGATAACCTCCAACGATGAAAATCTAATGAACCAGCTCGTGCCGGCGATTCAGAATCTTCACAACGCGCTGAACTCGATGTCTCTGGGGAATGATATTAAGGTTTCCACTGTCCACTCCATGGCGGTGCTTCGCCAATCGGAGCCGCCGTCTTCGGGGATGTTCCACCCGAACTATATGAGCGTGTTGAAGGGGCTATTGGAGTTCAACAATGCCACTGGATCGCCATTCACCATTAATCCATACCCCTACTTCGCCTATCGAAGCGATCCCCGGCCGGAGACTTTGGCTTTCTGTCTTTTTCAGCCCAACGCCGGCCGGCTCGACACGAACACCAATATCAAGTACATGAACATGTTCGACGCTCAGGTGGACGCCATAAGATCTGCGTTGAACTCGATGGGCTTCAAGAACGTAGAGATTGTGGTGGCGGAGACTGGGTGGCCGTACAAAGGAGACAGCGACGAGGTTGGTTCAAGTCTTGAGAATGCTAAGGCATTCAACGGTAATCTGATTGCTCACCTTCGTTCCATGGTTGGAACGCCATTGATGCCTGGAAAATCAGTTGATACGTATCTCTTTGCACTCTACGACGAAGATTTAAAGCCTGACCCTGGTTCTGAAAGATCTTTAGGATTGTTCAAGCCTGATCTTACAATGGCCTACGACGTTGGTCTTTCCAAAAATGGCCAGAGCACTGCAGCTAGTCCTAAAACACCAGTGACGACTCCATCATCCCCAGCAAATAATCCATCAACGAAAGCACCAACAAGTCCAAAACCAAAGGCAGGAGGAGGCTCATGGTGCTTGCCTAAGGGCGGTGTATCAGACGCCCAGCTTCAGGCCAATTTGGACTATGCCTGTGGAAGAGGGCTCGATTGCAGCGCGATTCAACCCGGTGGCGCGTGCTTCGAGCCAAACACCATAGCCTCACACGCCGCCTACGCCATGAACCTCTTCTTCCAAAACGCCGACCGGGATCCATGGACCTGTGACTTCTCACAATCCGCCACACTTTCCTCTAACAACCCAAGCTACAATGGCTGCAATTATCCTGGTGGGAGCACGTGA

mRNA sequence

ATGGCTACCACTCTTCTTCGCTCTACTGTTTTCTTCTTCTTTAACTTTCTATTGGCCACTGACATCGTCTATGCGCAATCGTTCATCGGAATAAACTACGGTCAAGTCGCCGATAACCTTCCACCTCCGTCCGCGACGGCGAAGCTACTTCAATCCACTTCAATCCAGAAAGTTCGACTCTACGGCGCTGATCCGGCCATCATCAAAGCCCTAGCAAACACAGGGATCGGAATTGTAATCGGCGCTGCGAACGGTGACATTCCAGCCCTAGCGTCCGACCCGAATTTCGCCAAAAATTGGGTTAACGCCAATGTAGCCCCCTTCCATCCAGCGAGTAAAATCATTCTCATCACGGTCGGCAACGAGGTGATAACCTCCAACGATGAAAATCTAATGAACCAGCTCGTGCCGGCGATTCAGAATCTTCACAACGCGCTGAACTCGATGTCTCTGGGGAATGATATTAAGGTTTCCACTGTCCACTCCATGGCGGTGCTTCGCCAATCGGAGCCGCCGTCTTCGGGGATGTTCCACCCGAACTATATGAGCGTGTTGAAGGGGCTATTGGAGTTCAACAATGCCACTGGATCGCCATTCACCATTAATCCATACCCCTACTTCGCCTATCGAAGCGATCCCCGGCCGGAGACTTTGGCTTTCTGTCTTTTTCAGCCCAACGCCGGCCGGCTCGACACGAACACCAATATCAAGTACATGAACATGTTCGACGCTCAGGTGGACGCCATAAGATCTGCGTTGAACTCGATGGGCTTCAAGAACGTAGAGATTGTGGTGGCGGAGACTGGGTGGCCGTACAAAGGAGACAGCGACGAGGTTGGTTCAAGTCTTGAGAATGCTAAGGCATTCAACGGTAATCTGATTGCTCACCTTCGTTCCATGGTTGGAACGCCATTGATGCCTGGAAAATCAGTTGATACGTATCTCTTTGCACTCTACGACGAAGATTTAAAGCCTGACCCTGGTTCTGAAAGATCTTTAGGATTGTTCAAGCCTGATCTTACAATGGCCTACGACGTTGGTCTTTCCAAAAATGGCCAGAGCACTGCAGCTAGTCCTAAAACACCAGTGACGACTCCATCATCCCCAGCAAATAATCCATCAACGAAAGCACCAACAAGTCCAAAACCAAAGGCAGGAGGAGGCTCATGGTGCTTGCCTAAGGGCGGTGTATCAGACGCCCAGCTTCAGGCCAATTTGGACTATGCCTGTGGAAGAGGGCTCGATTGCAGCGCGATTCAACCCGGTGGCGCGTGCTTCGAGCCAAACACCATAGCCTCACACGCCGCCTACGCCATGAACCTCTTCTTCCAAAACGCCGACCGGGATCCATGGACCTGTGACTTCTCACAATCCGCCACACTTTCCTCTAACAACCCAAGCTACAATGGCTGCAATTATCCTGGTGGGAGCACGTGA

Coding sequence (CDS)

ATGGCTACCACTCTTCTTCGCTCTACTGTTTTCTTCTTCTTTAACTTTCTATTGGCCACTGACATCGTCTATGCGCAATCGTTCATCGGAATAAACTACGGTCAAGTCGCCGATAACCTTCCACCTCCGTCCGCGACGGCGAAGCTACTTCAATCCACTTCAATCCAGAAAGTTCGACTCTACGGCGCTGATCCGGCCATCATCAAAGCCCTAGCAAACACAGGGATCGGAATTGTAATCGGCGCTGCGAACGGTGACATTCCAGCCCTAGCGTCCGACCCGAATTTCGCCAAAAATTGGGTTAACGCCAATGTAGCCCCCTTCCATCCAGCGAGTAAAATCATTCTCATCACGGTCGGCAACGAGGTGATAACCTCCAACGATGAAAATCTAATGAACCAGCTCGTGCCGGCGATTCAGAATCTTCACAACGCGCTGAACTCGATGTCTCTGGGGAATGATATTAAGGTTTCCACTGTCCACTCCATGGCGGTGCTTCGCCAATCGGAGCCGCCGTCTTCGGGGATGTTCCACCCGAACTATATGAGCGTGTTGAAGGGGCTATTGGAGTTCAACAATGCCACTGGATCGCCATTCACCATTAATCCATACCCCTACTTCGCCTATCGAAGCGATCCCCGGCCGGAGACTTTGGCTTTCTGTCTTTTTCAGCCCAACGCCGGCCGGCTCGACACGAACACCAATATCAAGTACATGAACATGTTCGACGCTCAGGTGGACGCCATAAGATCTGCGTTGAACTCGATGGGCTTCAAGAACGTAGAGATTGTGGTGGCGGAGACTGGGTGGCCGTACAAAGGAGACAGCGACGAGGTTGGTTCAAGTCTTGAGAATGCTAAGGCATTCAACGGTAATCTGATTGCTCACCTTCGTTCCATGGTTGGAACGCCATTGATGCCTGGAAAATCAGTTGATACGTATCTCTTTGCACTCTACGACGAAGATTTAAAGCCTGACCCTGGTTCTGAAAGATCTTTAGGATTGTTCAAGCCTGATCTTACAATGGCCTACGACGTTGGTCTTTCCAAAAATGGCCAGAGCACTGCAGCTAGTCCTAAAACACCAGTGACGACTCCATCATCCCCAGCAAATAATCCATCAACGAAAGCACCAACAAGTCCAAAACCAAAGGCAGGAGGAGGCTCATGGTGCTTGCCTAAGGGCGGTGTATCAGACGCCCAGCTTCAGGCCAATTTGGACTATGCCTGTGGAAGAGGGCTCGATTGCAGCGCGATTCAACCCGGTGGCGCGTGCTTCGAGCCAAACACCATAGCCTCACACGCCGCCTACGCCATGAACCTCTTCTTCCAAAACGCCGACCGGGATCCATGGACCTGTGACTTCTCACAATCCGCCACACTTTCCTCTAACAACCCAAGCTACAATGGCTGCAATTATCCTGGTGGGAGCACGTGA

Protein sequence

MATTLLRSTVFFFFNFLLATDIVYAQSFIGINYGQVADNLPPPSATAKLLQSTSIQKVRLYGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFHPASKIILITVGNEVITSNDENLMNQLVPAIQNLHNALNSMSLGNDIKVSTVHSMAVLRQSEPPSSGMFHPNYMSVLKGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMNMFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDSDEVGSSLENAKAFNGNLIAHLRSMVGTPLMPGKSVDTYLFALYDEDLKPDPGSERSLGLFKPDLTMAYDVGLSKNGQSTAASPKTPVTTPSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAIQPGGACFEPNTIASHAAYAMNLFFQNADRDPWTCDFSQSATLSSNNPSYNGCNYPGGST
Homology
BLAST of Lsi10G010210 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 628.2 bits (1619), Expect = 7.4e-179
Identity = 309/471 (65.61%), Postives = 374/471 (79.41%), Query Frame = 0

Query: 6   LRSTVFFFFNFLLATDIVYAQSFIGINYGQVADNLPPPSATAKLLQSTSIQKVRLYGADP 65
           L  +++F   FL      +A+ FIG+NYGQVADNLPPPS T KLLQSTSIQKVRLYGADP
Sbjct: 3   LSISIYFLLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADP 62

Query: 66  AIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFHPASKIILITVGNEVIT 125
           AIIKALA TG+GIVIGAANGD+P+LASDPN A  W+N+NV PF+PASKI+LITVGNE++ 
Sbjct: 63  AIIKALAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILM 122

Query: 126 SNDENLMNQLVPAIQNLHNALNSMSLGNDIKVSTVHSMAVLRQSEPPSSGMFHPNYMSVL 185
           SND NL+NQL+PA+QN+  AL ++SLG  IKVSTV+SM VL  S+PPSSG F   Y + L
Sbjct: 123 SNDPNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGL 182

Query: 186 KGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMNMFDAQ 245
           KG+L+F + TGSPF INPYP+FAY+SDPRPETLAFCLF+PNAGR+D+ T IKY NMFDAQ
Sbjct: 183 KGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQ 242

Query: 246 VDAIRSALNSMGFKNVEIVVAETGWPYKGDSDEVGSSLENAKAFNGNLIAHLRSMVGTPL 305
           VDA+ SAL SMGF+ VEIVVAETGW  +GD++EVG+S++NAKA+NGNLIAHLRSMVGTPL
Sbjct: 243 VDAVHSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPL 302

Query: 306 MPGKSVDTYLFALYDEDLKPDPGSERSLGLFKPDLTMAYDVGLSKNGQSTAASPKTPVTT 365
           MPGK VDTY+FALYDE+LKP P SER+ GLFK DL+M YDVGL+K+              
Sbjct: 303 MPGKPVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAKS-------------- 362

Query: 366 PSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAIQPGGAC 425
            SS +  PS K  +S         WC+PK G ++ +LQA+LD+ACG G+DC AIQPGGAC
Sbjct: 363 -SSSSQTPSGKVTSS--------GWCVPKKGATNEELQASLDWACGHGIDCGAIQPGGAC 422

Query: 426 FEPNTIASHAAYAMNLFFQNADRDPWTCDFSQSATLSSNNPSYNGCNYPGG 477
           FEPN + SHAAYAMN++FQ + + P  CDFS++AT++S NPSYN C YPGG
Sbjct: 423 FEPNNVVSHAAYAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYPGG 450

BLAST of Lsi10G010210 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 5.5e-166
Identity = 293/479 (61.17%), Postives = 364/479 (75.99%), Query Frame = 0

Query: 1   MATTLLRSTVFFFFNFLLATDIVYA--QSFIGINYGQVADNLPPPSATAKLLQSTSIQKV 60
           MA  +  S++ F   FLL  +   A  QSF+G+NYGQ++DNLP   AT  LL+ST+IQKV
Sbjct: 1   MAANVQTSSLLFLV-FLLLQNFYSANSQSFLGVNYGQLSDNLPSLQATVNLLKSTTIQKV 60

Query: 61  RLYGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFHPASKIILIT 120
           RL+GA+PA+IKA ANTG+ IVIG  NGDIP LAS+PN A  +V +NV  F+PAS II IT
Sbjct: 61  RLFGAEPAVIKAFANTGVEIVIGFDNGDIPTLASNPNVASQFVKSNVMSFYPASNIIAIT 120

Query: 121 VGNEVITSNDENLMNQLVPAIQNLHNALNSMSLGNDIKVSTVHSMAVLRQSEPPSSGMFH 180
           VGNEV+TS D+ L++QL+PA+QN+ NALN+ SLG  +KVSTVH+MAVL QS PPSSG+F+
Sbjct: 121 VGNEVLTSGDQKLISQLLPAMQNVQNALNAASLGGKVKVSTVHAMAVLSQSYPPSSGVFN 180

Query: 181 PNYMSVLKGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKY 240
           P     +K LL+F +A  +PF I+PYPYFAY++ P P+TLAFCLFQPNAG++D+    KY
Sbjct: 181 PGLGDTMKALLQFQSANDAPFMISPYPYFAYKNQPTPDTLAFCLFQPNAGQVDSGNGHKY 240

Query: 241 MNMFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDSDEVGSSLENAKAFNGNLIAHLR 300
            NMFDAQVDA+ SALN+MGFK++EIVVAETGWP+ GDS+EVG SL+NAKA+ GNLI HL+
Sbjct: 241 TNMFDAQVDAVHSALNAMGFKDIEIVVAETGWPHGGDSNEVGPSLDNAKAYVGNLINHLK 300

Query: 301 SMVGTPLMPGKSVDTYLFALYDEDLKPDPGSERSLGLFKPDLTMAYDVGLSKNGQSTAAS 360
           S VGTPLMPGKS+DTYLF+LYDED K    SE+  GLFKPD +  YDVGL KN Q     
Sbjct: 301 SKVGTPLMPGKSIDTYLFSLYDEDKKTGASSEKYFGLFKPDGSTTYDVGLLKNTQ----- 360

Query: 361 PKTPVTTPSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSA 420
                        NP+T A  +P PKA  GSWC+PK GVSD QL  N++YACG+G+DC  
Sbjct: 361 -------------NPTTPATPTPTPKA-AGSWCVPKPGVSDDQLTGNINYACGQGIDCGP 420

Query: 421 IQPGGACFEPNTIASHAAYAMNLFFQNADRDPWTCDFSQSATLSSNNPSYNGCNYPGGS 478
           IQPGGACFEPNT+ +HAAY MNL++Q+A R+ W CDFSQ+ATL++ NPSY  CN+P GS
Sbjct: 421 IQPGGACFEPNTVKAHAAYVMNLYYQSAGRNSWNCDFSQTATLTNTNPSYGACNFPSGS 459

BLAST of Lsi10G010210 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 3.4e-99
Identity = 190/451 (42.13%), Postives = 282/451 (62.53%), Query Frame = 0

Query: 29  IGINYGQVADNLPPPSATAKLLQS-TSIQKVRLYGADPAIIKALANTGIGIVIGAANGDI 88
           IG+NYG  ADNLP P++ A  L + T+I +V+L+ A+P  I A A T I + +   N  +
Sbjct: 26  IGVNYGANADNLPSPTSVATFLATKTTIDRVKLFDANPTFISAFAGTPISLAVSLPNSAL 85

Query: 89  PALASDP---NFAKNWVNANVAPFHPASKIILITVGNEVITSNDENLMNQLVPAIQNLHN 148
           PALA      + A++W+ AN++P+ PA+ + L+  GNE++ S D NL+  L+PA++ L  
Sbjct: 86  PALADKATGLDAARSWIRANLSPYVPATNVTLLLAGNEILLSTDTNLILSLLPAMRRLAQ 145

Query: 149 ALNSMSLGNDIKVSTVHSMAVLRQSEP-PSSGMFHPNYMSVL-KGLLEFNNATGSPFTIN 208
           AL +  L   ++V+T H + +L  S+  PS+  F   Y + L   +L+F+  TGSPF +N
Sbjct: 146 ALKAEGL-TGVRVTTPHYLGILAPSDGIPSNASFRAGYNTKLFPAMLQFHRDTGSPFMVN 205

Query: 209 PYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMNMFDAQVDAIRSALNSMGFKNVE 268
           PYPYF+Y    RPETL + LF+PN+G  D  T + Y +M DAQ+DAI +A+  +G+ +V+
Sbjct: 206 PYPYFSY----RPETLNYALFRPNSGIYDPATKLNYTSMLDAQMDAIYTAMKKLGYGDVD 265

Query: 269 IVVAETGWPYKGDSDEVGSSLENAKAFNGNLIAHLRSMVGTPLMPGKSVDTYLFALYDED 328
           I V E GWP + +  ++G  ++ A+ FN  +I    S  GTPLMP ++ +TYLF+L+DE+
Sbjct: 266 IAVGEAGWPTQAEPGQIGVGVQEARDFNEGMIRVCSSGKGTPLMPNRTFETYLFSLFDEN 325

Query: 329 LKPDPGSERSLGLFKPDLTMAYDVGLSKNGQSTAASPKTPVTTPSSPANNPSTKAPTSPK 388
            KP P +ER  GLF PD T  YD+GL ++G S A +P        +P+ NPS K    P 
Sbjct: 326 QKPGPIAERHFGLFNPDFTPVYDLGLLRDGASVAPTPS------PNPSPNPSPK----PA 385

Query: 389 PKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAIQPGGACFEPNTIASHAAYAMNLF 448
           P +GGG WC+ K G +   LQ N++YACG  +DC  IQ GGACF PN++ +HA+Y MN +
Sbjct: 386 P-SGGGKWCVAKDGANGTDLQNNINYACG-FVDCKPIQSGGACFSPNSLQAHASYVMNAY 445

Query: 449 FQNADRDPWTCDFSQSATLSSNNPSYNGCNY 474
           +Q        CDF  +  ++S++PSY GC Y
Sbjct: 446 YQANGHTDLACDFKGTGIVTSSDPSYGGCKY 459

BLAST of Lsi10G010210 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 3.4e-91
Identity = 186/469 (39.66%), Postives = 277/469 (59.06%), Query Frame = 0

Query: 10  VFFFFNFLLATDIVYAQSFIGINYGQVADNLPPPSATAKLLQSTSIQKVRLYGADPAIIK 69
           VF+ F  +LA       S IGI YG+ ADNLP P+  ++L+Q  +I+ VR+Y A+  ++K
Sbjct: 8   VFWIFVSILAFLNFGMASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLK 67

Query: 70  ALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFHPASKIILITVGNEVITSNDE 129
           A ANTGI ++IG  N D+ A A   +    W++ N+ P++P++KI  I+VG EV T   +
Sbjct: 68  AFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEV-TEAPD 127

Query: 130 NLMNQLVPAIQNLHNALNSMSLGNDIKVSTVHSMAVLRQSEPPSSGMFHPNYMSVLKGLL 189
           N    ++PA++N+H AL    L   IK+S+ HS+A+L +S PPSS  F   + + LK +L
Sbjct: 128 NATGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFLKPML 187

Query: 190 EFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMNMFDAQVDAI 249
           EF     SPF I+ YPY+AYR       L + LF+ ++  +D  T + Y NMFDAQ+DAI
Sbjct: 188 EFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAI 247

Query: 250 RSALNSMGFKNVEIVVAETGWPYKGDSDEVGSSLENAKAFNGNLIAHLRSMVGTPLMPGK 309
             AL +M FK V+++V E+GWP KG   E  ++ ENA A+N NLI H+    GTP  PG+
Sbjct: 248 YFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGE 307

Query: 310 SVDTYLFALYDEDLKPDPGSERSLGLFKPDLTMAYDVGLSKNGQSTAASPKTPVTTPSSP 369
            +D YLF+L++E+ KP   SER+ G+F  + T  Y +  +    +  +   +   T  SP
Sbjct: 308 EIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSP 367

Query: 370 ANNPSTKAPTSPKPKAGGG----SWCLPKGGVSDAQLQANLDYACGRG-LDCSAIQPGGA 429
           +++P     ++     GGG     WC+     S  +LQ  LD+ACG G +DCSA+QP   
Sbjct: 368 SSSPIINGNSTVTIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQP 427

Query: 430 CFEPNTIASHAAYAMNLFFQNADRDPWTCDFSQSATLSSNNPSYNGCNY 474
           CFEP+T+ SHA+YA N ++Q +      C F+ ++     +PSY  C Y
Sbjct: 428 CFEPDTVLSHASYAFNTYYQQSGASSIDCSFNGASVEVDKDPSYGNCLY 475

BLAST of Lsi10G010210 vs. ExPASy Swiss-Prot
Match: Q8L868 (Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860 PE=2 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 5.6e-86
Identity = 164/358 (45.81%), Postives = 230/358 (64.25%), Query Frame = 0

Query: 3   TTLLRSTVFFFFNFLLATDIVYAQSFIGINYGQVADNLPPPSATAKLLQSTSIQKVRLYG 62
           T+  RS++ F  +  L   +    + IG+NYGQ+ DNLP P+    L++S    KV+LY 
Sbjct: 4   TSFHRSSLLFLISLTLII-LPTTTTSIGVNYGQIGDNLPSPTDVIPLIKSIGATKVKLYD 63

Query: 63  ADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFHPASKIILITVGNE 122
           A+P I+KA +NTGI  +IG  N  +  +  DP+ A  W+  NV PF PA+ I  IT+GNE
Sbjct: 64  ANPQILKAFSNTGIEFIIGLGNEYLSKM-KDPSKALTWIKQNVTPFLPATNITCITIGNE 123

Query: 123 VITSNDENLMNQLVPAIQNLHNALNSMSLGNDIKVSTVHSMAVLRQSEPPSSGMFHPNYM 182
           ++  ND +L   L+PA+Q +H+AL +  L + I V+T HS+++L+ S PPS+G F P+ +
Sbjct: 124 ILALNDSSLTTNLLPAMQGVHSALITAGLSDQISVTTAHSLSILKSSFPPSAGEFQPDLL 183

Query: 183 SVLKGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMNMF 242
             L  +LEF+  T SPF IN YP+FAY+ +P+   L F LFQPN G +D  T   Y NM 
Sbjct: 184 DSLTPILEFHRKTDSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNML 243

Query: 243 DAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDSDEVGSSLENAKAFNGNLIAHLRS--M 302
            AQ+DA+ SAL + GFK++ + ++ETGWP KGD DEVG++ ENAK +NGNLI  + S   
Sbjct: 244 FAQIDAVYSALAAAGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMMSGKK 303

Query: 303 VGTPLMPGKSVDTYLFALYDEDLKPDPGSERSLGLFKPDLTMAYDVGLSKNGQSTAAS 359
             TPL P   +  Y+FAL++E+LKP P SER+ GLFKPD T AY +G + N     AS
Sbjct: 304 TKTPLKPNNDLSIYVFALFNENLKPGPTSERNYGLFKPDGTQAYSLGFALNDVVRGAS 359

BLAST of Lsi10G010210 vs. ExPASy TrEMBL
Match: A0A5A7TJN0 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold242G00090 PE=3 SV=1)

HSP 1 Score: 902.9 bits (2332), Expect = 5.7e-259
Identity = 454/479 (94.78%), Postives = 465/479 (97.08%), Query Frame = 0

Query: 1   MATTLLRSTVFFFFNFLLATDIVYAQSFIGINYGQVADNLPPPSATAKLLQSTSIQKVRL 60
           MA+TLL STVF FF+FLLATDIVY+QSFIGINYGQVADNLPPPSATAKLLQSTSI+KVRL
Sbjct: 1   MASTLLPSTVFVFFSFLLATDIVYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60

Query: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFHPASKIILITVG 120
           YGADPAIIKALANTGIGIVIGAANGDIPALA+DPNFAKNWVNANVAPFHPASKIILITVG
Sbjct: 61  YGADPAIIKALANTGIGIVIGAANGDIPALAADPNFAKNWVNANVAPFHPASKIILITVG 120

Query: 121 NEVITSNDENLMNQLVPAIQNLHNALNSMSLGNDIKVSTVHSMAVLRQSEPPSSGMFHPN 180
           NEVITSN ENLMNQLVPAIQN+ NALNSMSLG DIKVSTVHSMAVLRQSEPPSSGMFHPN
Sbjct: 121 NEVITSNQENLMNQLVPAIQNIQNALNSMSLG-DIKVSTVHSMAVLRQSEPPSSGMFHPN 180

Query: 181 YMSVLKGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240
           YM+VLK LLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN
Sbjct: 181 YMTVLKELLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240

Query: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDSDEVGSSLENAKAFNGNLIAHLRSM 300
           MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGD+DEVG SLENAKAFNGNLIAHLRSM
Sbjct: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDNDEVGPSLENAKAFNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYLFALYDEDLKPDPGSERSLGLFKPDLTMAYDVGLSKNGQSTAASPK 360
           VGTPLMPGKSVDTYLFALYDEDLKP PGSER+ GLFKPDLTM YDVGLSKNGQST +SPK
Sbjct: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKNGQSTPSSPK 360

Query: 361 -TPVTTPSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAI 420
            TPVTTPSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAI
Sbjct: 361 TTPVTTPSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAI 420

Query: 421 QPGGACFEPNTIASHAAYAMNLFFQNADRDPWTCDFSQSATLSSNNPSYNGCNYPGGST 479
           QPGGACFEPNTIASHAAYAMNLFFQN  RDPWTCDFSQSATLSSNNPSY+GCNYPGGST
Sbjct: 421 QPGGACFEPNTIASHAAYAMNLFFQNGGRDPWTCDFSQSATLSSNNPSYDGCNYPGGST 478

BLAST of Lsi10G010210 vs. ExPASy TrEMBL
Match: A0A1S3CRM8 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103503956 PE=3 SV=1)

HSP 1 Score: 902.9 bits (2332), Expect = 5.7e-259
Identity = 454/479 (94.78%), Postives = 465/479 (97.08%), Query Frame = 0

Query: 1   MATTLLRSTVFFFFNFLLATDIVYAQSFIGINYGQVADNLPPPSATAKLLQSTSIQKVRL 60
           MA+TLL STVF FF+FLLATDIVY+QSFIGINYGQVADNLPPPSATAKLLQSTSI+KVRL
Sbjct: 1   MASTLLPSTVFVFFSFLLATDIVYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60

Query: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFHPASKIILITVG 120
           YGADPAIIKALANTGIGIVIGAANGDIPALA+DPNFAKNWVNANVAPFHPASKIILITVG
Sbjct: 61  YGADPAIIKALANTGIGIVIGAANGDIPALAADPNFAKNWVNANVAPFHPASKIILITVG 120

Query: 121 NEVITSNDENLMNQLVPAIQNLHNALNSMSLGNDIKVSTVHSMAVLRQSEPPSSGMFHPN 180
           NEVITSN ENLMNQLVPAIQN+ NALNSMSLG DIKVSTVHSMAVLRQSEPPSSGMFHPN
Sbjct: 121 NEVITSNQENLMNQLVPAIQNIQNALNSMSLG-DIKVSTVHSMAVLRQSEPPSSGMFHPN 180

Query: 181 YMSVLKGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240
           YM+VLK LLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN
Sbjct: 181 YMTVLKELLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240

Query: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDSDEVGSSLENAKAFNGNLIAHLRSM 300
           MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGD+DEVG SLENAKAFNGNLIAHLRSM
Sbjct: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDNDEVGPSLENAKAFNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYLFALYDEDLKPDPGSERSLGLFKPDLTMAYDVGLSKNGQSTAASPK 360
           VGTPLMPGKSVDTYLFALYDEDLKP PGSER+ GLFKPDLTM YDVGLSKNGQST +SPK
Sbjct: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKNGQSTPSSPK 360

Query: 361 -TPVTTPSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAI 420
            TPVTTPSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAI
Sbjct: 361 TTPVTTPSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAI 420

Query: 421 QPGGACFEPNTIASHAAYAMNLFFQNADRDPWTCDFSQSATLSSNNPSYNGCNYPGGST 479
           QPGGACFEPNTIASHAAYAMNLFFQN  RDPWTCDFSQSATLSSNNPSY+GCNYPGGST
Sbjct: 421 QPGGACFEPNTIASHAAYAMNLFFQNGGRDPWTCDFSQSATLSSNNPSYDGCNYPGGST 478

BLAST of Lsi10G010210 vs. ExPASy TrEMBL
Match: A0A0A0LG31 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G855430 PE=3 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 7.5e-259
Identity = 452/479 (94.36%), Postives = 465/479 (97.08%), Query Frame = 0

Query: 1   MATTLLRSTVFFFFNFLLATDIVYAQSFIGINYGQVADNLPPPSATAKLLQSTSIQKVRL 60
           MA+T+L ST+F FF FLLAT+IVY+QSFIGINYGQVADNLPPPSATAKLLQSTSI+KVRL
Sbjct: 1   MASTILPSTIFVFFTFLLATEIVYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60

Query: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFHPASKIILITVG 120
           YGADPAIIKALANTGIGIVIGAANGDIPALA+DPNFAKNWVNANVAPFHPASKIILITVG
Sbjct: 61  YGADPAIIKALANTGIGIVIGAANGDIPALAADPNFAKNWVNANVAPFHPASKIILITVG 120

Query: 121 NEVITSNDENLMNQLVPAIQNLHNALNSMSLGNDIKVSTVHSMAVLRQSEPPSSGMFHPN 180
           NEVITSN +NLMNQLVPAIQN+ NALNSMSLG DIKVSTVHSMAVLRQSEPPSSGMFHPN
Sbjct: 121 NEVITSNQDNLMNQLVPAIQNIQNALNSMSLG-DIKVSTVHSMAVLRQSEPPSSGMFHPN 180

Query: 181 YMSVLKGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240
           YM+VLK LLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN
Sbjct: 181 YMTVLKELLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240

Query: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDSDEVGSSLENAKAFNGNLIAHLRSM 300
           MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGD+DEVGSSLENAKAFNGNLIAHLRSM
Sbjct: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDNDEVGSSLENAKAFNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYLFALYDEDLKPDPGSERSLGLFKPDLTMAYDVGLSKNGQSTAASPK 360
           VGTPLMPGKSVDTYLFALYDEDLKP PGSER+ GLFKPDLTMAYDVGLSKNGQST  SPK
Sbjct: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMAYDVGLSKNGQSTPTSPK 360

Query: 361 -TPVTTPSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAI 420
            TPVTTPSSPANNPSTK+PTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAI
Sbjct: 361 TTPVTTPSSPANNPSTKSPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAI 420

Query: 421 QPGGACFEPNTIASHAAYAMNLFFQNADRDPWTCDFSQSATLSSNNPSYNGCNYPGGST 479
           QPGGACFEPNTIASHAAYAMNLFFQN  RDPWTCDFSQSATLSSNNPSYNGCNYPGGST
Sbjct: 421 QPGGACFEPNTIASHAAYAMNLFFQNGGRDPWTCDFSQSATLSSNNPSYNGCNYPGGST 478

BLAST of Lsi10G010210 vs. ExPASy TrEMBL
Match: A0A6J1CLC6 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111012336 PE=3 SV=1)

HSP 1 Score: 875.5 bits (2261), Expect = 9.8e-251
Identity = 436/478 (91.21%), Postives = 454/478 (94.98%), Query Frame = 0

Query: 1   MATTLLRSTVFFFFNFLLATDIVYAQSFIGINYGQVADNLPPPSATAKLLQSTSIQKVRL 60
           MA+TLLR+TVFFF N LL  D  Y+QSFIGINYGQVADNLPPPSATAKLLQSTSI+KVRL
Sbjct: 1   MASTLLRATVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60

Query: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFHPASKIILITVG 120
           YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFA+NWVNANVAPF+PASKIILITVG
Sbjct: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVG 120

Query: 121 NEVITSNDENLMNQLVPAIQNLHNALNSMSLGNDIKVSTVHSMAVLRQSEPPSSGMFHPN 180
           NEVITSND+NLMNQL+PAIQNL NALNS+SLG++IKVSTVHSMAVLRQSEPPSS +FHPN
Sbjct: 121 NEVITSNDQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPN 180

Query: 181 YMSVLKGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240
           YMSVLKGLLEFN+ATGSPFTINPYPYFAY+SDPRPETLAFCLFQPNAGRLDTNTNIKYMN
Sbjct: 181 YMSVLKGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240

Query: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDSDEVGSSLENAKAFNGNLIAHLRSM 300
           MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGD  EVG  LENAKAFNGNLIAHLRSM
Sbjct: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYLFALYDEDLKPDPGSERSLGLFKPDLTMAYDVGLSKNGQSTAASPK 360
           VGTPLMPGKSVDTYLFALYDEDLKP PGSER+ GLFKPDLTM YDVGLSK+GQST ASPK
Sbjct: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPK 360

Query: 361 TPVTTPSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAIQ 420
           TP TTPSSPA NPSTKAP  PKPKAGGGSWC+P GGVSDAQLQANLDYACGRGLDCSAIQ
Sbjct: 361 TPATTPSSPA-NPSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAIQ 420

Query: 421 PGGACFEPNTIASHAAYAMNLFFQNADRDPWTCDFSQSATLSSNNPSYNGCNYPGGST 479
           PGGACFEPNTIASHAAYAMNLFFQ A RDPWTCDFS +ATLSSNNPSYNGCNYPGGST
Sbjct: 421 PGGACFEPNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNYPGGST 477

BLAST of Lsi10G010210 vs. ExPASy TrEMBL
Match: A0A6J1J4P1 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111481285 PE=3 SV=1)

HSP 1 Score: 867.5 bits (2240), Expect = 2.7e-248
Identity = 435/478 (91.00%), Postives = 452/478 (94.56%), Query Frame = 0

Query: 1   MATTLLRSTVFFFFNFLLATDIVYAQSFIGINYGQVADNLPPPSATAKLLQSTSIQKVRL 60
           MAT LLRST FFF  FLL  +I  +QSFIGINYG+VADNLPPPSATAKLLQSTSI+KVRL
Sbjct: 1   MATALLRSTAFFFVYFLLIFNIADSQSFIGINYGEVADNLPPPSATAKLLQSTSIEKVRL 60

Query: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFHPASKIILITVG 120
           YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPF PASKIILITVG
Sbjct: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFSPASKIILITVG 120

Query: 121 NEVITSNDENLMNQLVPAIQNLHNALNSMSLGNDIKVSTVHSMAVLRQSEPPSSGMFHPN 180
           NEVITSND+NLMNQLVPAIQNLHNALNSMSLG DIKVSTVHSMAVLRQSEPPSS +FHPN
Sbjct: 121 NEVITSNDQNLMNQLVPAIQNLHNALNSMSLG-DIKVSTVHSMAVLRQSEPPSSALFHPN 180

Query: 181 YMSVLKGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240
           YMSVLKGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN
Sbjct: 181 YMSVLKGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240

Query: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDSDEVGSSLENAKAFNGNLIAHLRSM 300
           MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKG+ DEVG S+ENAKAFNGNLIAHLRSM
Sbjct: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGNGDEVGPSVENAKAFNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYLFALYDEDLKPDPGSERSLGLFKPDLTMAYDVGLSKNGQSTAASPK 360
           VGTPLMPGKSVDTYLFALYDEDLKP PGSER+ GLFKPDLTM YD+GL KNGQ+T A+PK
Sbjct: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDIGLLKNGQNTPAAPK 360

Query: 361 TPVTTPSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAIQ 420
           TPVTTPSSPA NPS KAPTSPKP AGGGSWC+PKGGVS+AQLQANLDYACGRG+DCSAIQ
Sbjct: 361 TPVTTPSSPA-NPSAKAPTSPKPNAGGGSWCVPKGGVSNAQLQANLDYACGRGIDCSAIQ 420

Query: 421 PGGACFEPNTIASHAAYAMNLFFQNADRDPWTCDFSQSATLSSNNPSYNGCNYPGGST 479
           PGGACFEPNTIA+HA YAMNLF+Q A R PWTCDFSQSATLSS NPSYNGCNYPGGST
Sbjct: 421 PGGACFEPNTIAAHATYAMNLFYQTAGRYPWTCDFSQSATLSSKNPSYNGCNYPGGST 476

BLAST of Lsi10G010210 vs. NCBI nr
Match: XP_038905742.1 (glucan endo-1,3-beta-glucosidase 7-like [Benincasa hispida])

HSP 1 Score: 913.7 bits (2360), Expect = 6.7e-262
Identity = 455/478 (95.19%), Postives = 465/478 (97.28%), Query Frame = 0

Query: 1   MATTLLRSTVFFFFNFLLATDIVYAQSFIGINYGQVADNLPPPSATAKLLQSTSIQKVRL 60
           MA TLLRSTVFFFF+FLLATDIVY+QSFIGINYGQVADNLPPPSATAKLLQSTSI+KVRL
Sbjct: 1   MANTLLRSTVFFFFSFLLATDIVYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60

Query: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFHPASKIILITVG 120
           YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPF+PASKIILITVG
Sbjct: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFYPASKIILITVG 120

Query: 121 NEVITSNDENLMNQLVPAIQNLHNALNSMSLGNDIKVSTVHSMAVLRQSEPPSSGMFHPN 180
           NEVITSNDENLMNQLVPAIQNL NALNSMSLG DIKVSTVHSMAVLRQSEPPSSGMFHPN
Sbjct: 121 NEVITSNDENLMNQLVPAIQNLQNALNSMSLGKDIKVSTVHSMAVLRQSEPPSSGMFHPN 180

Query: 181 YMSVLKGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240
           YMSVLKGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN
Sbjct: 181 YMSVLKGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240

Query: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDSDEVGSSLENAKAFNGNLIAHLRSM 300
           MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGD DEVG SLENAKAFNGNLIAHLRSM
Sbjct: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGDEVGPSLENAKAFNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYLFALYDEDLKPDPGSERSLGLFKPDLTMAYDVGLSKNGQSTAASPK 360
           VGTPLMPGKSVDTYLFALYDEDLKP PGSER+ GLFKPDLTM Y+VGLSK+GQST A  K
Sbjct: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYNVGLSKDGQSTPAGSK 360

Query: 361 TPVTTPSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAIQ 420
           TPVTTPSSPA NPSTKAPT+PKPKAGGGSWC+PKGGVSDAQLQANLDYACGRGLDCSAIQ
Sbjct: 361 TPVTTPSSPATNPSTKAPTTPKPKAGGGSWCVPKGGVSDAQLQANLDYACGRGLDCSAIQ 420

Query: 421 PGGACFEPNTIASHAAYAMNLFFQNADRDPWTCDFSQSATLSSNNPSYNGCNYPGGST 479
           PGGACFEPNTIASHAAYAMNLFFQNADRDPWTCDFSQ+ATLSSNNPSYN CNYPGGST
Sbjct: 421 PGGACFEPNTIASHAAYAMNLFFQNADRDPWTCDFSQTATLSSNNPSYNSCNYPGGST 478

BLAST of Lsi10G010210 vs. NCBI nr
Match: XP_008466580.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 7 [Cucumis melo] >KAA0041635.1 glucan endo-1,3-beta-glucosidase 7 [Cucumis melo var. makuwa] >TYK19623.1 glucan endo-1,3-beta-glucosidase 7 [Cucumis melo var. makuwa])

HSP 1 Score: 902.9 bits (2332), Expect = 1.2e-258
Identity = 454/479 (94.78%), Postives = 465/479 (97.08%), Query Frame = 0

Query: 1   MATTLLRSTVFFFFNFLLATDIVYAQSFIGINYGQVADNLPPPSATAKLLQSTSIQKVRL 60
           MA+TLL STVF FF+FLLATDIVY+QSFIGINYGQVADNLPPPSATAKLLQSTSI+KVRL
Sbjct: 1   MASTLLPSTVFVFFSFLLATDIVYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60

Query: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFHPASKIILITVG 120
           YGADPAIIKALANTGIGIVIGAANGDIPALA+DPNFAKNWVNANVAPFHPASKIILITVG
Sbjct: 61  YGADPAIIKALANTGIGIVIGAANGDIPALAADPNFAKNWVNANVAPFHPASKIILITVG 120

Query: 121 NEVITSNDENLMNQLVPAIQNLHNALNSMSLGNDIKVSTVHSMAVLRQSEPPSSGMFHPN 180
           NEVITSN ENLMNQLVPAIQN+ NALNSMSLG DIKVSTVHSMAVLRQSEPPSSGMFHPN
Sbjct: 121 NEVITSNQENLMNQLVPAIQNIQNALNSMSLG-DIKVSTVHSMAVLRQSEPPSSGMFHPN 180

Query: 181 YMSVLKGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240
           YM+VLK LLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN
Sbjct: 181 YMTVLKELLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240

Query: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDSDEVGSSLENAKAFNGNLIAHLRSM 300
           MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGD+DEVG SLENAKAFNGNLIAHLRSM
Sbjct: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDNDEVGPSLENAKAFNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYLFALYDEDLKPDPGSERSLGLFKPDLTMAYDVGLSKNGQSTAASPK 360
           VGTPLMPGKSVDTYLFALYDEDLKP PGSER+ GLFKPDLTM YDVGLSKNGQST +SPK
Sbjct: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKNGQSTPSSPK 360

Query: 361 -TPVTTPSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAI 420
            TPVTTPSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAI
Sbjct: 361 TTPVTTPSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAI 420

Query: 421 QPGGACFEPNTIASHAAYAMNLFFQNADRDPWTCDFSQSATLSSNNPSYNGCNYPGGST 479
           QPGGACFEPNTIASHAAYAMNLFFQN  RDPWTCDFSQSATLSSNNPSY+GCNYPGGST
Sbjct: 421 QPGGACFEPNTIASHAAYAMNLFFQNGGRDPWTCDFSQSATLSSNNPSYDGCNYPGGST 478

BLAST of Lsi10G010210 vs. NCBI nr
Match: XP_004147788.1 (glucan endo-1,3-beta-glucosidase 7 [Cucumis sativus] >KGN59949.1 hypothetical protein Csa_002257 [Cucumis sativus])

HSP 1 Score: 902.5 bits (2331), Expect = 1.5e-258
Identity = 452/479 (94.36%), Postives = 465/479 (97.08%), Query Frame = 0

Query: 1   MATTLLRSTVFFFFNFLLATDIVYAQSFIGINYGQVADNLPPPSATAKLLQSTSIQKVRL 60
           MA+T+L ST+F FF FLLAT+IVY+QSFIGINYGQVADNLPPPSATAKLLQSTSI+KVRL
Sbjct: 1   MASTILPSTIFVFFTFLLATEIVYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60

Query: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFHPASKIILITVG 120
           YGADPAIIKALANTGIGIVIGAANGDIPALA+DPNFAKNWVNANVAPFHPASKIILITVG
Sbjct: 61  YGADPAIIKALANTGIGIVIGAANGDIPALAADPNFAKNWVNANVAPFHPASKIILITVG 120

Query: 121 NEVITSNDENLMNQLVPAIQNLHNALNSMSLGNDIKVSTVHSMAVLRQSEPPSSGMFHPN 180
           NEVITSN +NLMNQLVPAIQN+ NALNSMSLG DIKVSTVHSMAVLRQSEPPSSGMFHPN
Sbjct: 121 NEVITSNQDNLMNQLVPAIQNIQNALNSMSLG-DIKVSTVHSMAVLRQSEPPSSGMFHPN 180

Query: 181 YMSVLKGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240
           YM+VLK LLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN
Sbjct: 181 YMTVLKELLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240

Query: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDSDEVGSSLENAKAFNGNLIAHLRSM 300
           MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGD+DEVGSSLENAKAFNGNLIAHLRSM
Sbjct: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDNDEVGSSLENAKAFNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYLFALYDEDLKPDPGSERSLGLFKPDLTMAYDVGLSKNGQSTAASPK 360
           VGTPLMPGKSVDTYLFALYDEDLKP PGSER+ GLFKPDLTMAYDVGLSKNGQST  SPK
Sbjct: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMAYDVGLSKNGQSTPTSPK 360

Query: 361 -TPVTTPSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAI 420
            TPVTTPSSPANNPSTK+PTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAI
Sbjct: 361 TTPVTTPSSPANNPSTKSPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAI 420

Query: 421 QPGGACFEPNTIASHAAYAMNLFFQNADRDPWTCDFSQSATLSSNNPSYNGCNYPGGST 479
           QPGGACFEPNTIASHAAYAMNLFFQN  RDPWTCDFSQSATLSSNNPSYNGCNYPGGST
Sbjct: 421 QPGGACFEPNTIASHAAYAMNLFFQNGGRDPWTCDFSQSATLSSNNPSYNGCNYPGGST 478

BLAST of Lsi10G010210 vs. NCBI nr
Match: XP_022142141.1 (glucan endo-1,3-beta-glucosidase 7-like [Momordica charantia])

HSP 1 Score: 875.5 bits (2261), Expect = 2.0e-250
Identity = 436/478 (91.21%), Postives = 454/478 (94.98%), Query Frame = 0

Query: 1   MATTLLRSTVFFFFNFLLATDIVYAQSFIGINYGQVADNLPPPSATAKLLQSTSIQKVRL 60
           MA+TLLR+TVFFF N LL  D  Y+QSFIGINYGQVADNLPPPSATAKLLQSTSI+KVRL
Sbjct: 1   MASTLLRATVFFFLNILLVADFAYSQSFIGINYGQVADNLPPPSATAKLLQSTSIEKVRL 60

Query: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFHPASKIILITVG 120
           YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFA+NWVNANVAPF+PASKIILITVG
Sbjct: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAQNWVNANVAPFYPASKIILITVG 120

Query: 121 NEVITSNDENLMNQLVPAIQNLHNALNSMSLGNDIKVSTVHSMAVLRQSEPPSSGMFHPN 180
           NEVITSND+NLMNQL+PAIQNL NALNS+SLG++IKVSTVHSMAVLRQSEPPSS +FHPN
Sbjct: 121 NEVITSNDQNLMNQLLPAIQNLQNALNSISLGSNIKVSTVHSMAVLRQSEPPSSAVFHPN 180

Query: 181 YMSVLKGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240
           YMSVLKGLLEFN+ATGSPFTINPYPYFAY+SDPRPETLAFCLFQPNAGRLDTNTNIKYMN
Sbjct: 181 YMSVLKGLLEFNSATGSPFTINPYPYFAYQSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240

Query: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDSDEVGSSLENAKAFNGNLIAHLRSM 300
           MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGD  EVG  LENAKAFNGNLIAHLRSM
Sbjct: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDGGEVGPGLENAKAFNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYLFALYDEDLKPDPGSERSLGLFKPDLTMAYDVGLSKNGQSTAASPK 360
           VGTPLMPGKSVDTYLFALYDEDLKP PGSER+ GLFKPDLTM YDVGLSK+GQST ASPK
Sbjct: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDVGLSKSGQSTPASPK 360

Query: 361 TPVTTPSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAIQ 420
           TP TTPSSPA NPSTKAP  PKPKAGGGSWC+P GGVSDAQLQANLDYACGRGLDCSAIQ
Sbjct: 361 TPATTPSSPA-NPSTKAPAGPKPKAGGGSWCVPMGGVSDAQLQANLDYACGRGLDCSAIQ 420

Query: 421 PGGACFEPNTIASHAAYAMNLFFQNADRDPWTCDFSQSATLSSNNPSYNGCNYPGGST 479
           PGGACFEPNTIASHAAYAMNLFFQ A RDPWTCDFS +ATLSSNNPSYNGCNYPGGST
Sbjct: 421 PGGACFEPNTIASHAAYAMNLFFQAAGRDPWTCDFSHTATLSSNNPSYNGCNYPGGST 477

BLAST of Lsi10G010210 vs. NCBI nr
Match: XP_022982479.1 (glucan endo-1,3-beta-glucosidase 7-like [Cucurbita maxima])

HSP 1 Score: 867.5 bits (2240), Expect = 5.5e-248
Identity = 435/478 (91.00%), Postives = 452/478 (94.56%), Query Frame = 0

Query: 1   MATTLLRSTVFFFFNFLLATDIVYAQSFIGINYGQVADNLPPPSATAKLLQSTSIQKVRL 60
           MAT LLRST FFF  FLL  +I  +QSFIGINYG+VADNLPPPSATAKLLQSTSI+KVRL
Sbjct: 1   MATALLRSTAFFFVYFLLIFNIADSQSFIGINYGEVADNLPPPSATAKLLQSTSIEKVRL 60

Query: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFHPASKIILITVG 120
           YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPF PASKIILITVG
Sbjct: 61  YGADPAIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFSPASKIILITVG 120

Query: 121 NEVITSNDENLMNQLVPAIQNLHNALNSMSLGNDIKVSTVHSMAVLRQSEPPSSGMFHPN 180
           NEVITSND+NLMNQLVPAIQNLHNALNSMSLG DIKVSTVHSMAVLRQSEPPSS +FHPN
Sbjct: 121 NEVITSNDQNLMNQLVPAIQNLHNALNSMSLG-DIKVSTVHSMAVLRQSEPPSSALFHPN 180

Query: 181 YMSVLKGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240
           YMSVLKGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN
Sbjct: 181 YMSVLKGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMN 240

Query: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGDSDEVGSSLENAKAFNGNLIAHLRSM 300
           MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKG+ DEVG S+ENAKAFNGNLIAHLRSM
Sbjct: 241 MFDAQVDAIRSALNSMGFKNVEIVVAETGWPYKGNGDEVGPSVENAKAFNGNLIAHLRSM 300

Query: 301 VGTPLMPGKSVDTYLFALYDEDLKPDPGSERSLGLFKPDLTMAYDVGLSKNGQSTAASPK 360
           VGTPLMPGKSVDTYLFALYDEDLKP PGSER+ GLFKPDLTM YD+GL KNGQ+T A+PK
Sbjct: 301 VGTPLMPGKSVDTYLFALYDEDLKPGPGSERAFGLFKPDLTMTYDIGLLKNGQNTPAAPK 360

Query: 361 TPVTTPSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAIQ 420
           TPVTTPSSPA NPS KAPTSPKP AGGGSWC+PKGGVS+AQLQANLDYACGRG+DCSAIQ
Sbjct: 361 TPVTTPSSPA-NPSAKAPTSPKPNAGGGSWCVPKGGVSNAQLQANLDYACGRGIDCSAIQ 420

Query: 421 PGGACFEPNTIASHAAYAMNLFFQNADRDPWTCDFSQSATLSSNNPSYNGCNYPGGST 479
           PGGACFEPNTIA+HA YAMNLF+Q A R PWTCDFSQSATLSS NPSYNGCNYPGGST
Sbjct: 421 PGGACFEPNTIAAHATYAMNLFYQTAGRYPWTCDFSQSATLSSKNPSYNGCNYPGGST 476

BLAST of Lsi10G010210 vs. TAIR 10
Match: AT4G34480.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 628.2 bits (1619), Expect = 5.2e-180
Identity = 309/471 (65.61%), Postives = 374/471 (79.41%), Query Frame = 0

Query: 6   LRSTVFFFFNFLLATDIVYAQSFIGINYGQVADNLPPPSATAKLLQSTSIQKVRLYGADP 65
           L  +++F   FL      +A+ FIG+NYGQVADNLPPPS T KLLQSTSIQKVRLYGADP
Sbjct: 3   LSISIYFLLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADP 62

Query: 66  AIIKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFHPASKIILITVGNEVIT 125
           AIIKALA TG+GIVIGAANGD+P+LASDPN A  W+N+NV PF+PASKI+LITVGNE++ 
Sbjct: 63  AIIKALAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNEILM 122

Query: 126 SNDENLMNQLVPAIQNLHNALNSMSLGNDIKVSTVHSMAVLRQSEPPSSGMFHPNYMSVL 185
           SND NL+NQL+PA+QN+  AL ++SLG  IKVSTV+SM VL  S+PPSSG F   Y + L
Sbjct: 123 SNDPNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGL 182

Query: 186 KGLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMNMFDAQ 245
           KG+L+F + TGSPF INPYP+FAY+SDPRPETLAFCLF+PNAGR+D+ T IKY NMFDAQ
Sbjct: 183 KGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQ 242

Query: 246 VDAIRSALNSMGFKNVEIVVAETGWPYKGDSDEVGSSLENAKAFNGNLIAHLRSMVGTPL 305
           VDA+ SAL SMGF+ VEIVVAETGW  +GD++EVG+S++NAKA+NGNLIAHLRSMVGTPL
Sbjct: 243 VDAVHSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPL 302

Query: 306 MPGKSVDTYLFALYDEDLKPDPGSERSLGLFKPDLTMAYDVGLSKNGQSTAASPKTPVTT 365
           MPGK VDTY+FALYDE+LKP P SER+ GLFK DL+M YDVGL+K+              
Sbjct: 303 MPGKPVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGLAKS-------------- 362

Query: 366 PSSPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAIQPGGAC 425
            SS +  PS K  +S         WC+PK G ++ +LQA+LD+ACG G+DC AIQPGGAC
Sbjct: 363 -SSSSQTPSGKVTSS--------GWCVPKKGATNEELQASLDWACGHGIDCGAIQPGGAC 422

Query: 426 FEPNTIASHAAYAMNLFFQNADRDPWTCDFSQSATLSSNNPSYNGCNYPGG 477
           FEPN + SHAAYAMN++FQ + + P  CDFS++AT++S NPSYN C YPGG
Sbjct: 423 FEPNNVVSHAAYAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYPGG 450

BLAST of Lsi10G010210 vs. TAIR 10
Match: AT2G16230.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 595.5 bits (1534), Expect = 3.8e-170
Identity = 294/467 (62.96%), Postives = 358/467 (76.66%), Query Frame = 0

Query: 8   STVFFFFNFLLATDIVYAQSFIGINYGQVADNLPPPSATAKLLQSTSIQKVRLYGADPAI 67
           S +F     L +     AQSFIG+NYG ++DNLPPPS TAKLLQSTSIQKVRLY AD +I
Sbjct: 4   SILFLLLFILFSISPSNAQSFIGVNYGLLSDNLPPPSQTAKLLQSTSIQKVRLYNADSSI 63

Query: 68  IKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFHPASKIILITVGNEVITSN 127
           I +L  TGIGIVIG ANGD+P++ASD N A  W+N+NV PF+PAS IILI VGNEV+ SN
Sbjct: 64  ITSLVGTGIGIVIGVANGDLPSIASDLNIASQWINSNVLPFYPASNIILINVGNEVLLSN 123

Query: 128 DENLMNQLVPAIQNLHNALNSMSLGNDIKVSTVHSMAVLRQSEPPSSGMFHPNYMSVLKG 187
           D NL+NQL+PA+QN+  AL ++SLG  IKVSTVH+M VL  SEPPS+G F P+Y + LKG
Sbjct: 124 DLNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVHAMTVLGNSEPPSAGSFAPSYQAGLKG 183

Query: 188 LLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMNMFDAQVD 247
           +L+F + TGSPF INPYP+FAY+SDPRPETLAFCLFQPN GR+D+NT IKYMNMFDAQVD
Sbjct: 184 ILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFQPNPGRVDSNTGIKYMNMFDAQVD 243

Query: 248 AIRSALNSMGFKNVEIVVAETGWPYKGDSDEVGSSLENAKAFNGNLIAHLRSMVGTPLMP 307
           A+ SAL S+GF+ VE++VAETGWP  GDS+EVG S+ENAKA+NGNLIAHLRSMVGTPLMP
Sbjct: 244 AVHSALKSIGFEKVEVLVAETGWPSTGDSNEVGPSVENAKAYNGNLIAHLRSMVGTPLMP 303

Query: 308 GKSVDTYLFALYDEDLKPDPGSERSLGLFKPDLTMAYDVGLSKNGQSTAASPKTPVTTPS 367
           GKS+DTY+FAL+DE+LKP P  E+S GLFKPDL+MAYD+GL+K             TT S
Sbjct: 304 GKSIDTYIFALFDENLKPGPSFEQSFGLFKPDLSMAYDIGLTK-------------TTSS 363

Query: 368 SPANNPSTKAPTSPKPKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAIQPGGACFE 427
             + +P     TS         WC+PK   +  QLQ +LD+ CG+G+DC  I PGG CFE
Sbjct: 364 QTSQSPQLGKVTSM-------GWCVPKEDATQEQLQDSLDWVCGQGIDCGPIMPGGVCFE 423

Query: 428 PNTIASHAAYAMNLFFQNADRDPWTCDFSQSATLSSNNPSYNGCNYP 475
           PN +ASH AYAMNL+FQ +  +P  CDFS++A ++S NPSY+ C YP
Sbjct: 424 PNNVASHTAYAMNLYFQKSPENPTDCDFSKTARITSENPSYSSCVYP 450

BLAST of Lsi10G010210 vs. TAIR 10
Match: AT5G42720.1 (Glycosyl hydrolase family 17 protein )

HSP 1 Score: 481.5 bits (1238), Expect = 7.9e-136
Identity = 239/377 (63.40%), Postives = 302/377 (80.11%), Query Frame = 0

Query: 8   STVFFFFNFLLATDIVYAQSFIGINYGQVADNLPPPSATAKLLQSTSIQKVRLYGADPAI 67
           S +  FF+ LL   +  +Q F+G+NYG  ADNLPPPSA+AKLLQST+ QKVRLYG+DPA+
Sbjct: 7   SLILLFFSCLL--HLSKSQPFLGVNYGLTADNLPPPSASAKLLQSTTFQKVRLYGSDPAV 66

Query: 68  IKALANTGIGIVIGAANGDIPALASDPNFAKNWVNANVAPFHPASKIILITVGNEVITSN 127
           IKALANTGI IVIGA+NGD+P LASDP+FA++WV  NV P++PASKI+LI VGNE+ +  
Sbjct: 67  IKALANTGIEIVIGASNGDVPGLASDPSFARSWVETNVVPYYPASKIVLIAVGNEITSFG 126

Query: 128 DENLMNQLVPAIQNLHNALNSMSL-GNDIKVSTVHSMAVLRQSEPPSSGMFHPNYMSVLK 187
           D +LM+QL+PA++N+  AL + SL G  IKVSTVH M+VL  S+PPS+ +F P +  +LK
Sbjct: 127 DNSLMSQLLPAMKNVQTALEAASLGGGKIKVSTVHIMSVLAGSDPPSTAVFKPEHADILK 186

Query: 188 GLLEFNNATGSPFTINPYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMNMFDAQV 247
           GLLEFN+ TGSPF +NPYP+FAY+ D RPETLA+CLFQ N GR+D N+N+KYMNMFDAQV
Sbjct: 187 GLLEFNSETGSPFAVNPYPFFAYQDDRRPETLAYCLFQANPGRVDPNSNLKYMNMFDAQV 246

Query: 248 DAIRSALNSMGFKNVEIVVAETGWPYKGDSDEVGSSLENAKAFNGNLIAHLRSMVGTPLM 307
           DA+ SALNSMGFK+VEI+VAETGWPYKGD +E G+++ENA+A+N NLIAHL+S  GTPLM
Sbjct: 247 DAVYSALNSMGFKDVEIMVAETGWPYKGDPEEAGATVENARAYNKNLIAHLKSGSGTPLM 306

Query: 308 PGKSVDTYLFALYDEDLKPDPGSERSLGLFKPDLTMAYDVGLSK--NGQSTAASPKTPVT 367
           PG+ +DTYLFALYDE+LKP  GSER+ GLF+PDLTM YD+GL+K  N   T+ +P +P  
Sbjct: 307 PGRVIDTYLFALYDENLKPGKGSERAFGLFRPDLTMTYDIGLTKTTNYNQTSMAPLSPTR 366

Query: 368 TPSSPANNPSTKAPTSP 382
               PA  P+ +   SP
Sbjct: 367 PRLPPAAAPTRQTLPSP 381

BLAST of Lsi10G010210 vs. TAIR 10
Match: AT5G24318.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 392.9 bits (1008), Expect = 3.7e-109
Identity = 200/451 (44.35%), Postives = 282/451 (62.53%), Query Frame = 0

Query: 25  AQSFIGINYGQVADNLPPPSATAK-LLQSTSIQKVRLYGADPAIIKALANTGIGIVIGAA 84
           +++ IG+NYG +A+NLPPP   A+ LL ST I ++RL+  DP I++A ANTGI + +   
Sbjct: 26  SEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDTDPQILQAFANTGIAVTVTVP 85

Query: 85  NGDIPALASDPNFAKNWVNANVAPFHPASKIILITVGNEVITSNDENLMNQLVPAIQNLH 144
           N  IP L ++ + AK W++ ++ P  P++ II I VGNEVI++ D  L+  L+PA+Q+LH
Sbjct: 86  NDQIPHL-TNLSSAKQWISDHIQPHFPSTNIIRILVGNEVISTADHLLIRTLIPAMQSLH 145

Query: 145 NALNSMSLGNDIKVSTVHSMAVLRQSEPPSSGMFHPNY-MSVLKGLLEFNNATGSPFTIN 204
            AL S SL   I++ST HS+  L  S PPSS  F   Y   VLK LL F  +T SPF +N
Sbjct: 146 TALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQVLKPLLSFLRSTSSPFVVN 205

Query: 205 PYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMNMFDAQVDAIRSALNSMGFKNVE 264
           PYP+F Y      ETL F LF+PN G  D +T + Y NM DAQ+D++ SA++ +GF +VE
Sbjct: 206 PYPFFGYSI----ETLDFALFRPNPGLFDQHTKLLYTNMLDAQLDSVYSAMDKLGFSDVE 265

Query: 265 IVVAETGWPYKGDSDEVGSSLENAKAFNGNLIAHLRSMVGTPLMPGKSVDTYLFALYDED 324
           IV+ E GWP +GD D++G  ++ A  FN NLIA + S  GTPLMP ++ +TY+FAL++E+
Sbjct: 266 IVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLMPNRTFETYIFALFNEN 325

Query: 325 LKPDPGSERSLGLFKPDLTMAYDVGLSKNGQSTAASPKTPVTTPSSPANNPSTKAPTSPK 384
           LK  P SER+ G+F+ DLT  YD+G+ +           PV  P SP    S+K      
Sbjct: 326 LKSGPTSERNFGIFRSDLTPIYDIGILR----PTFRSSDPVYNPRSPVRGSSSK------ 385

Query: 385 PKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAIQPGGACFEPNTIASHAAYAMNLF 444
                  WC+ K G     LQ N+DY CG GLDC  I  GG C+ PNT+ +H+ YAMNL+
Sbjct: 386 ------RWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKAHSKYAMNLY 445

Query: 445 FQNADRDPWTCDFSQSATLSSNNPSYNGCNY 474
           +Q  ++  + CDF  +  +++ +PSY  C Y
Sbjct: 446 YQTMEKHEFDCDFDNTGEITTIDPSYGNCEY 455

BLAST of Lsi10G010210 vs. TAIR 10
Match: AT5G24318.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 386.7 bits (992), Expect = 2.7e-107
Identity = 198/447 (44.30%), Postives = 280/447 (62.64%), Query Frame = 0

Query: 25  AQSFIGINYGQVADNLPPPSATAK-LLQSTSIQKVRLYGADPAIIKALANTGIGIVIGAA 84
           +++ IG+NYG +A+NLPPP   A+ LL ST I ++RL+  DP I++A ANTGI + +   
Sbjct: 26  SEASIGVNYGTLANNLPPPQQVAEFLLHSTVINRIRLFDTDPQILQAFANTGIAVTVTVP 85

Query: 85  NGDIPALASDPNFAKNWVNANVAPFHPASKIILITVGNEVITSNDENLMNQLVPAIQNLH 144
           N  IP L ++ + AK W++ ++ P  P++ II I VGNEVI++ D  L+  L+PA+Q+LH
Sbjct: 86  NDQIPHL-TNLSSAKQWISDHIQPHFPSTNIIRILVGNEVISTADHLLIRTLIPAMQSLH 145

Query: 145 NALNSMSLGNDIKVSTVHSMAVLRQSEPPSSGMFHPNY-MSVLKGLLEFNNATGSPFTIN 204
            AL S SL   I++ST HS+  L  S PPSS  F   Y   VLK LL F  +T SPF +N
Sbjct: 146 TALVSASLHRRIQISTPHSLGTLTNSTPPSSAKFRRGYDAQVLKPLLSFLRSTSSPFVVN 205

Query: 205 PYPYFAYRSDPRPETLAFCLFQPNAGRLDTNTNIKYMNMFDAQVDAIRSALNSMGFKNVE 264
           PYP+F Y      ETL F LF+PN G  D +T + Y NM DAQ+D++ SA++ +GF +VE
Sbjct: 206 PYPFFGYSI----ETLDFALFRPNPGLFDQHTKLLYTNMLDAQLDSVYSAMDKLGFSDVE 265

Query: 265 IVVAETGWPYKGDSDEVGSSLENAKAFNGNLIAHLRSMVGTPLMPGKSVDTYLFALYDED 324
           IV+ E GWP +GD D++G  ++ A  FN NLIA + S  GTPLMP ++ +TY+FAL++E+
Sbjct: 266 IVIGEIGWPSEGDIDQIGVDVDTAAEFNKNLIARVDSGTGTPLMPNRTFETYIFALFNEN 325

Query: 325 LKPDPGSERSLGLFKPDLTMAYDVGLSKNGQSTAASPKTPVTTPSSPANNPSTKAPTSPK 384
           LK  P SER+ G+F+ DLT  YD+G+ +           PV  P SP    S+K      
Sbjct: 326 LKSGPTSERNFGIFRSDLTPIYDIGILR----PTFRSSDPVYNPRSPVRGSSSK------ 385

Query: 385 PKAGGGSWCLPKGGVSDAQLQANLDYACGRGLDCSAIQPGGACFEPNTIASHAAYAMNLF 444
                  WC+ K G     LQ N+DY CG GLDC  I  GG C+ PNT+ +H+ YAMNL+
Sbjct: 386 ------RWCVTKAGAETVALQRNIDYVCGLGLDCRPINEGGLCYLPNTVKAHSKYAMNLY 445

Query: 445 FQNADRDPWTCDFSQSATLSSNNPSYN 470
           +Q  ++  + CDF  +  +++ +PS N
Sbjct: 446 YQTMEKHEFDCDFDNTGEITTIDPSMN 451

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M0697.4e-17965.61Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q94G865.5e-16661.17Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1[more]
P524093.4e-9942.13Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1[more]
Q8VYE53.4e-9139.66Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Q8L8685.6e-8645.81Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860... [more]
Match NameE-valueIdentityDescription
A0A5A7TJN05.7e-25994.78(1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3CRM85.7e-25994.78(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103503956 PE=3 SV... [more]
A0A0A0LG317.5e-25994.36(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_3G855430 PE=3... [more]
A0A6J1CLC69.8e-25191.21(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111012336 ... [more]
A0A6J1J4P12.7e-24891.00(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111481285 PE=... [more]
Match NameE-valueIdentityDescription
XP_038905742.16.7e-26295.19glucan endo-1,3-beta-glucosidase 7-like [Benincasa hispida][more]
XP_008466580.11.2e-25894.78PREDICTED: glucan endo-1,3-beta-glucosidase 7 [Cucumis melo] >KAA0041635.1 gluca... [more]
XP_004147788.11.5e-25894.36glucan endo-1,3-beta-glucosidase 7 [Cucumis sativus] >KGN59949.1 hypothetical pr... [more]
XP_022142141.12.0e-25091.21glucan endo-1,3-beta-glucosidase 7-like [Momordica charantia][more]
XP_022982479.15.5e-24891.00glucan endo-1,3-beta-glucosidase 7-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G34480.15.2e-18065.61O-Glycosyl hydrolases family 17 protein [more]
AT2G16230.13.8e-17062.96O-Glycosyl hydrolases family 17 protein [more]
AT5G42720.17.9e-13663.40Glycosyl hydrolase family 17 protein [more]
AT5G24318.13.7e-10944.35O-Glycosyl hydrolases family 17 protein [more]
AT5G24318.22.7e-10744.30O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 389..473
e-value: 3.8E-42
score: 156.0
IPR012946X8 domainPFAMPF07983X8coord: 390..460
e-value: 2.1E-19
score: 69.9
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 28..356
e-value: 7.4E-123
score: 411.6
NoneNo IPR availableGENE3D1.20.58.1040coord: 387..473
e-value: 8.0E-9
score: 37.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 351..379
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 351..390
NoneNo IPR availablePANTHERPTHR32227:SF16GLYCOSYL HYDROLASE FAMILY 17 PROTEINcoord: 5..464
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 29..346
e-value: 1.4E-82
score: 277.8
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 5..464
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 29..347

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G010210.1Lsi10G010210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds