Lsi10G010090 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi10G010090 vs. ExPASy Swiss-Prot
Match: Q84UC0 (Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase OS=Trichosanthes kirilowii OX=3677 GN=FAC PE=2 SV=1)

HSP 1 Score: 643.7 bits (1659), Expect = 1.4e-183
Identity = 303/394 (76.90%), Postives = 345/394 (87.56%), Query Frame = 0

Query: 1   MGGGEETEMRKQSRGRSNLALGKRITHAKPPFTITQIKKAIPSHCFQRSLYRSFSYIILD 60
           MGGGE  E+R  S   + LA G+RITHAKPPF+I+QIKKAIP HCFQRSLYRSFSY+I D
Sbjct: 1   MGGGEGIEVRSGS-SSTKLAFGERITHAKPPFSISQIKKAIPPHCFQRSLYRSFSYVIFD 60

Query: 61  SILLTTFYYIATTYFHSLPHPIHYLAWPLYWLSQGCVITGLWVIAHECGHHAFSNYQFLD 120
            I  +TFY+IA T FH LPHP+HYLAWPLYW  QG V TGLWVIAHECGH AFS+YQ +D
Sbjct: 61  FIFASTFYHIAATNFHRLPHPLHYLAWPLYWFCQGSVFTGLWVIAHECGHRAFSDYQLVD 120

Query: 121 DLLGFLLHSSFLIPYFSFKISHRRHHANTASLDRDEVFVPTPNSKIPWYFNHLTNPPARL 180
           D++GFLLH+SFLIPYFSFKISHRRHH+NTASL+RDEVFVP P +K+PWYF HLTNPPAR+
Sbjct: 121 DVVGFLLHTSFLIPYFSFKISHRRHHSNTASLERDEVFVPKPKAKMPWYFKHLTNPPARV 180

Query: 181 LIIFLTLILGWPMYLAFNSSGRFYDRFTSHYDPNSPIFSQKERLQVQISNAGVVTVWYLL 240
           LIIF+TL LGWPMYLAFN SGRFY+RFTSH+DPNSPIFS+ E LQV ISNAG+V VWYLL
Sbjct: 181 LIIFITLTLGWPMYLAFNISGRFYERFTSHFDPNSPIFSENEWLQVHISNAGIVAVWYLL 240

Query: 241 YKLAVAKGITWVICMYLLPLTVMNAFVVLISSLQHTHPSLPYYDTSEWDWLRGNLVTVDR 300
           YKLA AKGI WVI MY++P+T+MNAFVVLI+SLQHTHPS PYYD++EW+WLRGNLVT+DR
Sbjct: 241 YKLAAAKGIAWVIRMYVVPVTIMNAFVVLITSLQHTHPSFPYYDSTEWNWLRGNLVTLDR 300

Query: 301 DYGMILNKMFHNITNTHVIHHLFPSMPHYNAMEATKAVKQMLGEYYQFDETPILKAVWRE 360
           DYG ILNK+FHNIT+THV+HHLFPSMPHYNAMEAT+AVKQ+LGEYY FD TPI KA WRE
Sbjct: 301 DYG-ILNKVFHNITDTHVVHHLFPSMPHYNAMEATRAVKQVLGEYYHFDGTPIFKAAWRE 360

Query: 361 FRECVYVEADDEETKDGDSEASSSKGVFWFRNKL 395
           FREC+YVE D++E       +SSSKGVFWFRNKL
Sbjct: 361 FRECIYVEPDNDE-----GASSSSKGVFWFRNKL 387

BLAST of Lsi10G010090 vs. ExPASy Swiss-Prot
Match: Q9SP61 (Delta(12) acyl-lipid conjugase (11E,13E-forming) OS=Momordica charantia OX=3673 GN=FADX PE=1 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 2.2e-165
Identity = 271/394 (68.78%), Postives = 330/394 (83.76%), Query Frame = 0

Query: 2   GGGEETEMRKQSRGRSNLALGKRITHAKPPFTITQIKKAIPSHCFQRSLYRSFSYIILDS 61
           GGG +   +K   G+  L  G+RITHA+PPF+I+QIKKAIP HCFQRSL RSFSY++ D 
Sbjct: 13  GGGPK---KKMGPGQ-GLGPGERITHARPPFSISQIKKAIPPHCFQRSLRRSFSYLLSDI 72

Query: 62  ILLTTFYYIATTYFHSLPHP-IHYLAWPLYWLSQGCVITGLWVIAHECGHHAFSNYQFLD 121
            L++ FYY+A TYFH LPHP +HYLAWP+YW  QG V+TG+W IAH+CGHHAFS+YQ +D
Sbjct: 73  ALVSAFYYVADTYFHRLPHPLLHYLAWPVYWFCQGAVLTGMWGIAHDCGHHAFSDYQLVD 132

Query: 122 DLLGFLLHSSFLIPYFSFKISHRRHHANTASLDRDEVFVPTPNSKIPWYFNHLTNPPARL 181
           D++GFL+HS   +PYFSFKISHRRHH+NT+S+DRDEVFVP P +K+PWYF +LTNPPAR+
Sbjct: 133 DVVGFLIHSLVFVPYFSFKISHRRHHSNTSSVDRDEVFVPKPKAKMPWYFKYLTNPPARV 192

Query: 182 LIIFLTLILGWPMYLAFNSSGRFYDRFTSHYDPNSPIFSQKERLQVQISNAGVVTVWYLL 241
            IIF+TL LGWPMYL FN SGR+Y RFTSH+DPNSPIFS KER+ V ISNAG+V   YLL
Sbjct: 193 FIIFITLTLGWPMYLTFNISGRYYGRFTSHFDPNSPIFSPKERVLVHISNAGLVATGYLL 252

Query: 242 YKLAVAKGITWVICMYLLPLTVMNAFVVLISSLQHTHPSLPYYDTSEWDWLRGNLVTVDR 301
           Y++A+AKG+ W+I +Y +PL V+NA VVLI++LQHTHPS PYYD++EWDWLRGNLVTVDR
Sbjct: 253 YRIAMAKGVGWLIRLYGVPLIVLNACVVLITALQHTHPSFPYYDSTEWDWLRGNLVTVDR 312

Query: 302 DYGMILNKMFHNITNTHVIHHLFPSMPHYNAMEATKAVKQMLGEYYQFDETPILKAVWRE 361
           DYG I+N++FH+IT+THV+HHLFPSMPHYN  EAT A K++LGEYYQFD TPI KA WRE
Sbjct: 313 DYGPIMNRVFHHITDTHVVHHLFPSMPHYNGKEATVAAKRILGEYYQFDGTPIWKAAWRE 372

Query: 362 FRECVYVEADDEETKDGDSEASSSKGVFWFRNKL 395
           FRECVYVE D++   DG +  SSSKGVFW+ NKL
Sbjct: 373 FRECVYVEPDED---DGATSGSSSKGVFWYHNKL 399

BLAST of Lsi10G010090 vs. ExPASy Swiss-Prot
Match: Q8GZC3 (Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii OX=73154 GN=FAD2 PE=1 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 1.9e-148
Identity = 241/371 (64.96%), Postives = 298/371 (80.32%), Query Frame = 0

Query: 23  KRITHAKPPFTITQIKKAIPSHCFQRSLYRSFSYIILDSILLTTFYYIATTYFHSLPHPI 82
           KR+ H+KPPFT+ Q+KKAIP HCFQRS+ RSFSY++ D  +   FYYIAT YFH LP P+
Sbjct: 22  KRVPHSKPPFTLGQLKKAIPPHCFQRSVLRSFSYVVYDLTVAFIFYYIATNYFHLLPQPL 81

Query: 83  HYLAWPLYWLSQGCVITGLWVIAHECGHHAFSNYQFLDDLLGFLLHSSFLIPYFSFKISH 142
            Y+AWP+YW  QGCV+TG+WVIAHECGHHAFS+YQ LDD++G +LHS  L+PYFS+K SH
Sbjct: 82  SYVAWPIYWALQGCVLTGVWVIAHECGHHAFSDYQLLDDIVGLVLHSCLLVPYFSWKHSH 141

Query: 143 RRHHANTASLDRDEVFVPTPNSKIPWYFNHLTNPPARLLIIFLTLILGWPMYLAFNSSGR 202
           RRHH+NTASL+RDEVFVP   S I W+  +L NPP RL  + +TL LGWP+YLAFN SGR
Sbjct: 142 RRHHSNTASLERDEVFVPKKKSSIRWFSKYLNNPPGRLFTLTITLTLGWPLYLAFNVSGR 201

Query: 203 FYDRFTSHYDPNSPIFSQKERLQVQISNAGVVTVWYLLYKLAVAKGITWVICMYLLPLTV 262
            YDRF  HYDP  PI++ +ER ++ IS+AGV+ V + LY+LA AKG+ WVIC+Y +PL +
Sbjct: 202 PYDRFACHYDPYGPIYTDRERTEIYISDAGVLAVTFGLYRLAAAKGLAWVICVYGVPLLI 261

Query: 263 MNAFVVLISSLQHTHPSLPYYDTSEWDWLRGNLVTVDRDYGMILNKMFHNITNTHVIHHL 322
           +NAF+V+I+ LQHTHPS+P+YD+SEWDWLRG L TVDRDYG ILNK+FHNIT+THV HHL
Sbjct: 262 VNAFLVMITYLQHTHPSIPHYDSSEWDWLRGALATVDRDYG-ILNKVFHNITDTHVAHHL 321

Query: 323 FPSMPHYNAMEATKAVKQMLGEYYQFDETPILKAVWREFRECVYVEADDEETKDGDSEAS 382
           F +MPHY+AMEATKA+K +LGEYYQFD TP  KA+WRE +EC+YVEAD     DGD    
Sbjct: 322 FSTMPHYHAMEATKAIKPILGEYYQFDGTPFYKAMWREAKECIYVEAD-----DGD---- 381

Query: 383 SSKGVFWFRNK 394
            SKGV+W+  K
Sbjct: 382 ESKGVYWYNKK 382

BLAST of Lsi10G010090 vs. ExPASy Swiss-Prot
Match: Q84VT2 (Delta(12)-acyl-lipid-desaturase OS=Punica granatum OX=22663 GN=FAD12 PE=2 SV=2)

HSP 1 Score: 516.2 bits (1328), Expect = 3.4e-145
Identity = 228/372 (61.29%), Postives = 298/372 (80.11%), Query Frame = 0

Query: 23  KRITHAKPPFTITQIKKAIPSHCFQRSLYRSFSYIILDSILLTTFYYIATTYFHSLPHPI 82
           +R+  AKPPFT+++IKKAIP HCF+RSL +SFSY++ D  L+  FYY+ATTY  +LP P+
Sbjct: 26  QRVPSAKPPFTLSEIKKAIPPHCFKRSLLKSFSYVLYDLTLVAIFYYVATTYIDALPGPL 85

Query: 83  HYLAWPLYWLSQGCVITGLWVIAHECGHHAFSNYQFLDDLLGFLLHSSFLIPYFSFKISH 142
            Y AWP+YW  QGCV+TG+WVIAHECGHHAFS+YQ++DD +G +LHS+ L+PYFS+K SH
Sbjct: 86  RYAAWPVYWALQGCVLTGVWVIAHECGHHAFSDYQWVDDCVGLVLHSALLVPYFSWKYSH 145

Query: 143 RRHHANTASLDRDEVFVPTPNSKIPWYFNHLTNPPARLLIIFLTLILGWPMYLAFNSSGR 202
           RRHH+NT SL+RDEVFVP P SK+PW+  +L NPP R++ + +TL LGWP+YLA N SG 
Sbjct: 146 RRHHSNTGSLERDEVFVPKPKSKMPWFSKYLNNPPGRVMTLIVTLTLGWPLYLALNVSGW 205

Query: 203 FYDRFTSHYDPNSPIFSQKERLQVQISNAGVVTVWYLLYKLAVAKGITWVICMYLLPLTV 262
            YDRF  H+DP  PI++ +ERLQ+ IS+ G++   Y LYK+A A+G+ W++C+Y +PL +
Sbjct: 206 PYDRFACHFDPYGPIYTDRERLQIYISDVGIMAATYTLYKIAAARGLAWLVCVYGVPLLI 265

Query: 263 MNAFVVLISSLQHTHPSLPYYDTSEWDWLRGNLVTVDRDYGMILNKMFHNITNTHVIHHL 322
           +NAF+V I+ LQHTHP+LP+YD+SEWDWLRG L T DRDYG ILNK+FHNIT+THV HHL
Sbjct: 266 VNAFLVTITYLQHTHPALPHYDSSEWDWLRGALATADRDYG-ILNKVFHNITDTHVAHHL 325

Query: 323 FPSMPHYNAMEATKAVKQMLGEYYQFDETPILKAVWREFRECVYVEADDEETKDGDSEAS 382
           F +MPHY+AMEATKA+K +LG+YYQFD TP+ KA+WRE REC+YVE DD          +
Sbjct: 326 FSTMPHYHAMEATKAIKPILGDYYQFDGTPVYKAMWREARECLYVEPDD---------GA 385

Query: 383 SSKGVFWFRNKL 395
           +SKGVFW++  L
Sbjct: 386 NSKGVFWYKKNL 387

BLAST of Lsi10G010090 vs. ExPASy Swiss-Prot
Match: Q8GZC2 (Bifunctional desaturase/conjugase FADX OS=Vernicia fordii OX=73154 PE=1 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 4.9e-144
Identity = 231/371 (62.26%), Postives = 291/371 (78.44%), Query Frame = 0

Query: 23  KRITHAKPPFTITQIKKAIPSHCFQRSLYRSFSYIILDSILLTTFYYIATTYFHSLPHPI 82
           KR+ H KPPFT+ Q+K+AIPSHCF+RSL RSFSY++ D  L   FY IATTYFH LP PI
Sbjct: 25  KRVPHTKPPFTLGQLKQAIPSHCFKRSLLRSFSYVVYDLSLSFIFYSIATTYFHLLPSPI 84

Query: 83  HYLAWPLYWLSQGCVITGLWVIAHECGHHAFSNYQFLDDLLGFLLHSSFLIPYFSFKISH 142
            Y+AWP+YW  QGC++T +WV+ HECGHHAFS Y +LDD +G +LHSS L+PYFSFKISH
Sbjct: 85  TYIAWPVYWAFQGCILTSVWVLGHECGHHAFSEYNWLDDTIGLILHSSLLVPYFSFKISH 144

Query: 143 RRHHANTASLDRDEVFVPTPNSKIPWYFNHLTNPPARLLIIFLTLILGWPMYLAFNSSGR 202
           RRHH+N ASL+RDEVFVP   S IPWY  +L NPP R L +  TL +GWP+YLAFN SGR
Sbjct: 145 RRHHSNIASLERDEVFVPRLKSAIPWYSKYLNNPPGRALTLVATLFIGWPLYLAFNVSGR 204

Query: 203 FYDRFTSHYDPNSPIFSQKERLQVQISNAGVVTVWYLLYKLAVAKGITWVICMYLLPLTV 262
           +YDRF  HYDP SPI+S +ERLQ+ IS+A +    Y+LYK+A+AKG+ W++C+Y +PL +
Sbjct: 205 YYDRFACHYDPYSPIYSDRERLQIYISDAMIFVAAYVLYKIAMAKGLAWLVCIYGVPLLI 264

Query: 263 MNAFVVLISSLQHTHPSLPYYDTSEWDWLRGNLVTVDRDYGMILNKMFHNITNTHVIHHL 322
           +NA VV I+SLQHTH +LP+YD+SEWDWLRG L TVDRDYG + NK+FHN T+THVIHHL
Sbjct: 265 VNALVVTITSLQHTHVALPHYDSSEWDWLRGGLATVDRDYG-VFNKIFHNATDTHVIHHL 324

Query: 323 FPSMPHYNAMEATKAVKQMLGEYYQFDETPILKAVWREFRECVYVEADDEETKDGDSEAS 382
           F SMPHY+ +EAT+A+K +LG+YY FD+TPI  A+WRE +EC++VE D         E  
Sbjct: 325 FSSMPHYHGVEATRAIKPILGDYYLFDDTPIHVALWREAKECLFVEPD---------EGD 384

Query: 383 SSKGVFWFRNK 394
           ++ GVFW+ NK
Sbjct: 385 NNNGVFWYSNK 385

BLAST of Lsi10G010090 vs. ExPASy TrEMBL
Match: A0A5A7TJ17 (Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00030 PE=3 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 1.5e-193
Identity = 328/388 (84.54%), Postives = 353/388 (90.98%), Query Frame = 0

Query: 9   MRKQSRGRSNLALGKRITHAKPPFTITQIKKAIPSHCFQRSLYRSFSYIILDSILLTTFY 68
           MRK+S  RS LALG+RITH KPPFT TQIKK+IPSHCF RSLYRSFSY+I DSILL+TFY
Sbjct: 1   MRKRSPSRSKLALGERITHTKPPFTFTQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFY 60

Query: 69  YIATTYFHSLPHP--IHYLAWPLYWLSQGCVITGLWVIAHECGHHAFSNYQFLDDLLGFL 128
           Y+ATTYFH+LPHP  +HYLAWPLYWLSQG V TGLWVIAHECGHHAFS+YQFLDDL+GFL
Sbjct: 61  YVATTYFHTLPHPLLLHYLAWPLYWLSQGIVFTGLWVIAHECGHHAFSDYQFLDDLVGFL 120

Query: 129 LHSSFLIPYFSFKISHRRHHANTASLDRDEVFVPTPNSKIPWYFNHLTNPPARLLIIFLT 188
           LHSS LIPYFSFKISHRRHHANTASL+RDEVFVP   SKIPWYF HLTNPPARL II +T
Sbjct: 121 LHSSLLIPYFSFKISHRRHHANTASLNRDEVFVPKLKSKIPWYFKHLTNPPARLFIIVMT 180

Query: 189 LILGWPMYLAFNSSGRFYDRFTSHYDPNSPIFSQKERLQVQISNAGVVTVWYLLYKLAVA 248
           L LGWPMYLAFN+SGR YDRFTSHYDPNSPIF++KERLQVQISNAGV+T+ YLLYKLAV 
Sbjct: 181 LTLGWPMYLAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLAVT 240

Query: 249 KGITWVICMYLLPLTVMNAFVVLISSLQHTHPSLPYYDTSEWDWLRGNLVTVDRDYGMIL 308
           KGITWVI +YLLPLTVMN FVVLISSLQHTHPSLPYYD+S+WDWLRGNLVTVDRDYG IL
Sbjct: 241 KGITWVIRLYLLPLTVMNVFVVLISSLQHTHPSLPYYDSSQWDWLRGNLVTVDRDYGKIL 300

Query: 309 NKMFHNITNTHVIHHLFPSMPHYNAMEATKAVKQMLGEYYQFDETPILKAVWREFRECVY 368
           NKM HNIT+THVIHHLFPSMPHYNA EAT+AVK++LGEYYQFDETPILKA WREFRECVY
Sbjct: 301 NKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFRECVY 360

Query: 369 VEADDEETKDGDSEASSSKGVFWFRNKL 395
           VE DD E  +GDSE   SKGVFWFRNKL
Sbjct: 361 VEEDDNE--EGDSEGFRSKGVFWFRNKL 386

BLAST of Lsi10G010090 vs. ExPASy TrEMBL
Match: A0A1S3BMK7 (bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like OS=Cucumis melo OX=3656 GN=LOC103491678 PE=3 SV=1)

HSP 1 Score: 685.3 bits (1767), Expect = 1.5e-193
Identity = 328/388 (84.54%), Postives = 353/388 (90.98%), Query Frame = 0

Query: 9   MRKQSRGRSNLALGKRITHAKPPFTITQIKKAIPSHCFQRSLYRSFSYIILDSILLTTFY 68
           MRK+S  RS LALG+RITH KPPFT TQIKK+IPSHCF RSLYRSFSY+I DSILL+TFY
Sbjct: 1   MRKRSPSRSKLALGERITHTKPPFTFTQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFY 60

Query: 69  YIATTYFHSLPHP--IHYLAWPLYWLSQGCVITGLWVIAHECGHHAFSNYQFLDDLLGFL 128
           Y+ATTYFH+LPHP  +HYLAWPLYWLSQG V TGLWVIAHECGHHAFS+YQFLDDL+GFL
Sbjct: 61  YVATTYFHTLPHPLLLHYLAWPLYWLSQGIVFTGLWVIAHECGHHAFSDYQFLDDLVGFL 120

Query: 129 LHSSFLIPYFSFKISHRRHHANTASLDRDEVFVPTPNSKIPWYFNHLTNPPARLLIIFLT 188
           LHSS LIPYFSFKISHRRHHANTASL+RDEVFVP   SKIPWYF HLTNPPARL II +T
Sbjct: 121 LHSSLLIPYFSFKISHRRHHANTASLNRDEVFVPKLKSKIPWYFKHLTNPPARLFIIVMT 180

Query: 189 LILGWPMYLAFNSSGRFYDRFTSHYDPNSPIFSQKERLQVQISNAGVVTVWYLLYKLAVA 248
           L LGWPMYLAFN+SGR YDRFTSHYDPNSPIF++KERLQVQISNAGV+T+ YLLYKLAV 
Sbjct: 181 LTLGWPMYLAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLAVT 240

Query: 249 KGITWVICMYLLPLTVMNAFVVLISSLQHTHPSLPYYDTSEWDWLRGNLVTVDRDYGMIL 308
           KGITWVI +YLLPLTVMN FVVLISSLQHTHPSLPYYD+S+WDWLRGNLVTVDRDYG IL
Sbjct: 241 KGITWVIRLYLLPLTVMNVFVVLISSLQHTHPSLPYYDSSQWDWLRGNLVTVDRDYGKIL 300

Query: 309 NKMFHNITNTHVIHHLFPSMPHYNAMEATKAVKQMLGEYYQFDETPILKAVWREFRECVY 368
           NKM HNIT+THVIHHLFPSMPHYNA EAT+AVK++LGEYYQFDETPILKA WREFRECVY
Sbjct: 301 NKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFRECVY 360

Query: 369 VEADDEETKDGDSEASSSKGVFWFRNKL 395
           VE DD E  +GDSE   SKGVFWFRNKL
Sbjct: 361 VEEDDNE--EGDSEGFRSKGVFWFRNKL 386

BLAST of Lsi10G010090 vs. ExPASy TrEMBL
Match: A0A5D3DDB5 (Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00200 PE=3 SV=1)

HSP 1 Score: 684.1 bits (1764), Expect = 3.4e-193
Identity = 326/388 (84.02%), Postives = 352/388 (90.72%), Query Frame = 0

Query: 9   MRKQSRGRSNLALGKRITHAKPPFTITQIKKAIPSHCFQRSLYRSFSYIILDSILLTTFY 68
           MRK+S  RS LALG+RITH KPPFT TQIKK+IPSHCF RSLYRSFSY+I DSILL+TFY
Sbjct: 1   MRKRSPSRSKLALGERITHTKPPFTFTQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFY 60

Query: 69  YIATTYFHSLPHP--IHYLAWPLYWLSQGCVITGLWVIAHECGHHAFSNYQFLDDLLGFL 128
           Y+ATTYFH+LPHP  +HYLAWPLYWLSQG V TGLWVIAHECGHHAFS+YQFLDDL+GFL
Sbjct: 61  YVATTYFHTLPHPLLLHYLAWPLYWLSQGIVFTGLWVIAHECGHHAFSDYQFLDDLVGFL 120

Query: 129 LHSSFLIPYFSFKISHRRHHANTASLDRDEVFVPTPNSKIPWYFNHLTNPPARLLIIFLT 188
           LHSS LIPYFSFKISHRRHHANTASL+RDEVFVP P SKIPWYF HLTNPPARL II +T
Sbjct: 121 LHSSLLIPYFSFKISHRRHHANTASLNRDEVFVPKPKSKIPWYFKHLTNPPARLFIIVMT 180

Query: 189 LILGWPMYLAFNSSGRFYDRFTSHYDPNSPIFSQKERLQVQISNAGVVTVWYLLYKLAVA 248
           L LGWPMY+AFN+SGR YDRFTSHYDPNSPIF++KERLQVQISNAGV+T+ YLLYK AV 
Sbjct: 181 LTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKFAVT 240

Query: 249 KGITWVICMYLLPLTVMNAFVVLISSLQHTHPSLPYYDTSEWDWLRGNLVTVDRDYGMIL 308
           KGI WVI +YLLPLTVMN FVVLISSLQHTHPSLPYYD+S+WDWLRGNLVTVDRDYG IL
Sbjct: 241 KGIMWVIRIYLLPLTVMNVFVVLISSLQHTHPSLPYYDSSQWDWLRGNLVTVDRDYGKIL 300

Query: 309 NKMFHNITNTHVIHHLFPSMPHYNAMEATKAVKQMLGEYYQFDETPILKAVWREFRECVY 368
           NKM HNIT+THVIHHLFPSMPHYNA EAT+AVK++LGEYYQFDETPILKA WREFRECVY
Sbjct: 301 NKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFRECVY 360

Query: 369 VEADDEETKDGDSEASSSKGVFWFRNKL 395
           VE DD E  +GDSE   SKGVFWFRNKL
Sbjct: 361 VEEDDNE--EGDSEGFRSKGVFWFRNKL 386

BLAST of Lsi10G010090 vs. ExPASy TrEMBL
Match: A0A6J1JD33 (bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like OS=Cucurbita maxima OX=3661 GN=LOC111484610 PE=4 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 2.0e-180
Identity = 308/387 (79.59%), Postives = 338/387 (87.34%), Query Frame = 0

Query: 8   EMRKQSRGRSNLALGKRITHAKPPFTITQIKKAIPSHCFQRSLYRSFSYIILDSILLTTF 67
           EM+K++   S LALG+RITHAKPPFTITQIKKAIP  CFQRSLYRSFSY+I DSI ++ F
Sbjct: 2   EMQKRTPA-SKLALGERITHAKPPFTITQIKKAIPPRCFQRSLYRSFSYLIFDSIFVSIF 61

Query: 68  YYIATTYFHSLPHPIHYLAWPLYWLSQGCVITGLWVIAHECGHHAFSNYQFLDDLLGFLL 127
           Y+IATTYFH LPHP+HYLAWPLYW  QGCV T LWVIAHECGHHAF +YQF++ L+GFLL
Sbjct: 62  YHIATTYFHRLPHPLHYLAWPLYWFCQGCVFTSLWVIAHECGHHAFCDYQFVEHLVGFLL 121

Query: 128 HSSFLIPYFSFKISHRRHHANTASLDRDEVFVPTPNSKIPWYFNHLTNPPARLLIIFLTL 187
           HSS LIPYFSFK SHRRHH+NTASL+RDEVFVP P S++PWYF HLTNPPARLLI+FLTL
Sbjct: 122 HSSLLIPYFSFKFSHRRHHSNTASLERDEVFVPKPKSRMPWYFKHLTNPPARLLILFLTL 181

Query: 188 ILGWPMYLAFNSSGRFYDRFTSHYDPNSPIFSQKERLQVQISNAGVVTVWYLLYKLAVAK 247
            LGWPMYLAFNSSGRFYD FTSHY PNSPIFS KE LQV IS+AGVV  WYLLYKLA A+
Sbjct: 182 TLGWPMYLAFNSSGRFYDSFTSHYYPNSPIFSGKEWLQVHISSAGVVAFWYLLYKLAAAR 241

Query: 248 GITWVICMYLLPLTVMNAFVVLISSLQHTHPSLPYYDTSEWDWLRGNLVTVDRDYGMILN 307
           GI WVI +YLLPLTVM AFVVLISSLQHTHPSLPYYD+SEWDWLRGNLVTVDRDYG ILN
Sbjct: 242 GIAWVIRIYLLPLTVMYAFVVLISSLQHTHPSLPYYDSSEWDWLRGNLVTVDRDYG-ILN 301

Query: 308 KMFHNITNTHVIHHLFPSMPHYNAMEATKAVKQMLGEYYQFDETPILKAVWREFRECVYV 367
           K+FHNIT+THVIHHLFPSMPHYNA EAT+AVK++LGEYY FD TPI KA WREFREC+YV
Sbjct: 302 KVFHNITDTHVIHHLFPSMPHYNATEATRAVKEVLGEYYHFDGTPIFKAAWREFRECIYV 361

Query: 368 EADDEETKDGDSEASSSKGVFWFRNKL 395
           E D++E       AS SKGVFWFRN L
Sbjct: 362 EPDEDE----GGSASCSKGVFWFRNNL 382

BLAST of Lsi10G010090 vs. ExPASy TrEMBL
Match: A0A6J1FY47 (bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like OS=Cucurbita moschata OX=3662 GN=LOC111448352 PE=4 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 8.2e-179
Identity = 309/387 (79.84%), Postives = 338/387 (87.34%), Query Frame = 0

Query: 8   EMRKQSRGRSNLALGKRITHAKPPFTITQIKKAIPSHCFQRSLYRSFSYIILDSILLTTF 67
           EM+K++   S LALG+RITHAKPPFTITQIKKAIP  CFQRSLYRSFSY+I DSI ++ F
Sbjct: 2   EMQKRTPA-SKLALGERITHAKPPFTITQIKKAIPPRCFQRSLYRSFSYLIFDSIFVSIF 61

Query: 68  YYIATTYFHSLPHPIHYLAWPLYWLSQGCVITGLWVIAHECGHHAFSNYQFLDDLLGFLL 127
           Y+IATTYFH LPHP+H LAWPLYW  QGCV T LWVIAHECGHHAF +YQF++ LLGFLL
Sbjct: 62  YHIATTYFHRLPHPLHCLAWPLYWFCQGCVFTSLWVIAHECGHHAFCDYQFVEHLLGFLL 121

Query: 128 HSSFLIPYFSFKISHRRHHANTASLDRDEVFVPTPNSKIPWYFNHLTNPPARLLIIFLTL 187
           HSS LIPYFSFK SHRRHH+NTASL+RDEVFVP P S++PWYF HLTNPPARLLIIFLTL
Sbjct: 122 HSSLLIPYFSFKFSHRRHHSNTASLERDEVFVPKPKSRMPWYFKHLTNPPARLLIIFLTL 181

Query: 188 ILGWPMYLAFNSSGRFYDRFTSHYDPNSPIFSQKERLQVQISNAGVVTVWYLLYKLAVAK 247
            LG PMYLAFNSSGRFYDRFTSHY PNSPIFS KE LQV IS+AGVV  WYLLYKLA A+
Sbjct: 182 TLGRPMYLAFNSSGRFYDRFTSHYYPNSPIFSGKEWLQVHISSAGVVAFWYLLYKLAAAR 241

Query: 248 GITWVICMYLLPLTVMNAFVVLISSLQHTHPSLPYYDTSEWDWLRGNLVTVDRDYGMILN 307
           GI WVI +YLLPLTVM AFVVLISSLQHTHPSLPYYD+SEWDWLRGNLVTVDRD G ILN
Sbjct: 242 GIAWVIRIYLLPLTVMYAFVVLISSLQHTHPSLPYYDSSEWDWLRGNLVTVDRDNG-ILN 301

Query: 308 KMFHNITNTHVIHHLFPSMPHYNAMEATKAVKQMLGEYYQFDETPILKAVWREFRECVYV 367
           K+FHNIT+THVIHHLFPSMPHYNAMEAT+AVK++LGEYY FD TPILKA WREFREC+YV
Sbjct: 302 KVFHNITDTHVIHHLFPSMPHYNAMEATRAVKEVLGEYYHFDGTPILKAAWREFRECIYV 361

Query: 368 EADDEETKDGDSEASSSKGVFWFRNKL 395
           E D++E       A+ SKGVFWFRN L
Sbjct: 362 EPDEDE----GGSATCSKGVFWFRNNL 382

BLAST of Lsi10G010090 vs. NCBI nr
Match: XP_008449949.1 (PREDICTED: bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like [Cucumis melo] >KAA0041359.1 bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like [Cucumis melo var. makuwa])

HSP 1 Score: 685.3 bits (1767), Expect = 3.2e-193
Identity = 328/388 (84.54%), Postives = 353/388 (90.98%), Query Frame = 0

Query: 9   MRKQSRGRSNLALGKRITHAKPPFTITQIKKAIPSHCFQRSLYRSFSYIILDSILLTTFY 68
           MRK+S  RS LALG+RITH KPPFT TQIKK+IPSHCF RSLYRSFSY+I DSILL+TFY
Sbjct: 1   MRKRSPSRSKLALGERITHTKPPFTFTQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFY 60

Query: 69  YIATTYFHSLPHP--IHYLAWPLYWLSQGCVITGLWVIAHECGHHAFSNYQFLDDLLGFL 128
           Y+ATTYFH+LPHP  +HYLAWPLYWLSQG V TGLWVIAHECGHHAFS+YQFLDDL+GFL
Sbjct: 61  YVATTYFHTLPHPLLLHYLAWPLYWLSQGIVFTGLWVIAHECGHHAFSDYQFLDDLVGFL 120

Query: 129 LHSSFLIPYFSFKISHRRHHANTASLDRDEVFVPTPNSKIPWYFNHLTNPPARLLIIFLT 188
           LHSS LIPYFSFKISHRRHHANTASL+RDEVFVP   SKIPWYF HLTNPPARL II +T
Sbjct: 121 LHSSLLIPYFSFKISHRRHHANTASLNRDEVFVPKLKSKIPWYFKHLTNPPARLFIIVMT 180

Query: 189 LILGWPMYLAFNSSGRFYDRFTSHYDPNSPIFSQKERLQVQISNAGVVTVWYLLYKLAVA 248
           L LGWPMYLAFN+SGR YDRFTSHYDPNSPIF++KERLQVQISNAGV+T+ YLLYKLAV 
Sbjct: 181 LTLGWPMYLAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLAVT 240

Query: 249 KGITWVICMYLLPLTVMNAFVVLISSLQHTHPSLPYYDTSEWDWLRGNLVTVDRDYGMIL 308
           KGITWVI +YLLPLTVMN FVVLISSLQHTHPSLPYYD+S+WDWLRGNLVTVDRDYG IL
Sbjct: 241 KGITWVIRLYLLPLTVMNVFVVLISSLQHTHPSLPYYDSSQWDWLRGNLVTVDRDYGKIL 300

Query: 309 NKMFHNITNTHVIHHLFPSMPHYNAMEATKAVKQMLGEYYQFDETPILKAVWREFRECVY 368
           NKM HNIT+THVIHHLFPSMPHYNA EAT+AVK++LGEYYQFDETPILKA WREFRECVY
Sbjct: 301 NKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFRECVY 360

Query: 369 VEADDEETKDGDSEASSSKGVFWFRNKL 395
           VE DD E  +GDSE   SKGVFWFRNKL
Sbjct: 361 VEEDDNE--EGDSEGFRSKGVFWFRNKL 386

BLAST of Lsi10G010090 vs. NCBI nr
Match: TYK21657.1 (bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like [Cucumis melo var. makuwa])

HSP 1 Score: 684.1 bits (1764), Expect = 7.1e-193
Identity = 326/388 (84.02%), Postives = 352/388 (90.72%), Query Frame = 0

Query: 9   MRKQSRGRSNLALGKRITHAKPPFTITQIKKAIPSHCFQRSLYRSFSYIILDSILLTTFY 68
           MRK+S  RS LALG+RITH KPPFT TQIKK+IPSHCF RSLYRSFSY+I DSILL+TFY
Sbjct: 1   MRKRSPSRSKLALGERITHTKPPFTFTQIKKSIPSHCFNRSLYRSFSYVIFDSILLSTFY 60

Query: 69  YIATTYFHSLPHP--IHYLAWPLYWLSQGCVITGLWVIAHECGHHAFSNYQFLDDLLGFL 128
           Y+ATTYFH+LPHP  +HYLAWPLYWLSQG V TGLWVIAHECGHHAFS+YQFLDDL+GFL
Sbjct: 61  YVATTYFHTLPHPLLLHYLAWPLYWLSQGIVFTGLWVIAHECGHHAFSDYQFLDDLVGFL 120

Query: 129 LHSSFLIPYFSFKISHRRHHANTASLDRDEVFVPTPNSKIPWYFNHLTNPPARLLIIFLT 188
           LHSS LIPYFSFKISHRRHHANTASL+RDEVFVP P SKIPWYF HLTNPPARL II +T
Sbjct: 121 LHSSLLIPYFSFKISHRRHHANTASLNRDEVFVPKPKSKIPWYFKHLTNPPARLFIIVMT 180

Query: 189 LILGWPMYLAFNSSGRFYDRFTSHYDPNSPIFSQKERLQVQISNAGVVTVWYLLYKLAVA 248
           L LGWPMY+AFN+SGR YDRFTSHYDPNSPIF++KERLQVQISNAGV+T+ YLLYK AV 
Sbjct: 181 LTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKFAVT 240

Query: 249 KGITWVICMYLLPLTVMNAFVVLISSLQHTHPSLPYYDTSEWDWLRGNLVTVDRDYGMIL 308
           KGI WVI +YLLPLTVMN FVVLISSLQHTHPSLPYYD+S+WDWLRGNLVTVDRDYG IL
Sbjct: 241 KGIMWVIRIYLLPLTVMNVFVVLISSLQHTHPSLPYYDSSQWDWLRGNLVTVDRDYGKIL 300

Query: 309 NKMFHNITNTHVIHHLFPSMPHYNAMEATKAVKQMLGEYYQFDETPILKAVWREFRECVY 368
           NKM HNIT+THVIHHLFPSMPHYNA EAT+AVK++LGEYYQFDETPILKA WREFRECVY
Sbjct: 301 NKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFRECVY 360

Query: 369 VEADDEETKDGDSEASSSKGVFWFRNKL 395
           VE DD E  +GDSE   SKGVFWFRNKL
Sbjct: 361 VEEDDNE--EGDSEGFRSKGVFWFRNKL 386

BLAST of Lsi10G010090 vs. NCBI nr
Match: XP_011653570.2 (bifunctional fatty acid conjugase/Delta(12)-oleate desaturase [Cucumis sativus])

HSP 1 Score: 677.2 bits (1746), Expect = 8.7e-191
Identity = 327/393 (83.21%), Postives = 352/393 (89.57%), Query Frame = 0

Query: 5   EETEMRKQSRGRSNLALGKRITHAKPPFTITQIKKAIPSHCFQRSLYRSFSYIILDSILL 64
           ++ EMRK S  RS LALG+RITH KPPFTITQIKK+IPSHCF RSLYRSFSY+I DSILL
Sbjct: 28  QKIEMRKGSPNRSKLALGERITHTKPPFTITQIKKSIPSHCFNRSLYRSFSYVIFDSILL 87

Query: 65  TTFYYIATTYFHSL-PHP--IHYLAWPLYWLSQGCVITGLWVIAHECGHHAFSNYQFLDD 124
           +TFYY+ATTYFH+L  HP  +HYLAWPLYWLSQG V TG WVIAHECGHHAFS+YQFLDD
Sbjct: 88  STFYYVATTYFHTLSAHPLLLHYLAWPLYWLSQGIVFTGFWVIAHECGHHAFSDYQFLDD 147

Query: 125 LLGFLLHSSFLIPYFSFKISHRRHHANTASLDRDEVFVPTPNSKIPWYFNHLTNPPARLL 184
            LGFLLHSS LIPYFSFKISHRRHHANTASL RDEVFVP P SKIPWYF HLTNPPARL 
Sbjct: 148 FLGFLLHSSLLIPYFSFKISHRRHHANTASLHRDEVFVPKPKSKIPWYFKHLTNPPARLF 207

Query: 185 IIFLTLILGWPMYLAFNSSGRFYDRFTSHYDPNSPIFSQKERLQVQISNAGVVTVWYLLY 244
           II +TL LGWPMY+AFN+SGR YDRFTSHYDPNSPIF++KERLQVQISNAGV+T+ YLLY
Sbjct: 208 IIVMTLTLGWPMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLY 267

Query: 245 KLAVAKGITWVICMYLLPLTVMNAFVVLISSLQHTHPSLPYYDTSEWDWLRGNLVTVDRD 304
           KLAV KGITWVI +YLLPLTVMN FVVLIS LQHTH SLPYYD+S+WDWLRGNLVTVDRD
Sbjct: 268 KLAVTKGITWVIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRD 327

Query: 305 YGMILNKMFHNITNTHVIHHLFPSMPHYNAMEATKAVKQMLGEYYQFDETPILKAVWREF 364
           YG ILNKM HNIT+THVIHHLFPSMPHYNA EAT+AVK++LGEYYQFDETPILKA WREF
Sbjct: 328 YGKILNKMLHNITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREF 387

Query: 365 RECVYVEADDEETKDGDSEASSSKGVFWFRNKL 395
           RECVYVE DD E  +GDSEA  SKGVFWFRNKL
Sbjct: 388 RECVYVEEDDHE--EGDSEAFRSKGVFWFRNKL 418

BLAST of Lsi10G010090 vs. NCBI nr
Match: KAG6570368.1 (Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 646.4 bits (1666), Expect = 1.6e-181
Identity = 309/387 (79.84%), Postives = 341/387 (88.11%), Query Frame = 0

Query: 8   EMRKQSRGRSNLALGKRITHAKPPFTITQIKKAIPSHCFQRSLYRSFSYIILDSILLTTF 67
           EM+K++   S LALG+RITHAKPPFTITQIKKAIP  CFQRSLYRSFSY+I DSI ++ F
Sbjct: 2   EMQKRTPA-SKLALGERITHAKPPFTITQIKKAIPPRCFQRSLYRSFSYLIFDSIFVSIF 61

Query: 68  YYIATTYFHSLPHPIHYLAWPLYWLSQGCVITGLWVIAHECGHHAFSNYQFLDDLLGFLL 127
           Y+IATTYFH LPHP+HYLAWPLYW  QGCV T LWVIAHECGHHAF +YQF++ L+GFLL
Sbjct: 62  YHIATTYFHRLPHPLHYLAWPLYWFCQGCVFTSLWVIAHECGHHAFCDYQFVEHLVGFLL 121

Query: 128 HSSFLIPYFSFKISHRRHHANTASLDRDEVFVPTPNSKIPWYFNHLTNPPARLLIIFLTL 187
           HSS LIPYFSFK SHRRHH+NTASL+RDEVFVP P S++PWYF HLTNPPARLLI+FLTL
Sbjct: 122 HSSLLIPYFSFKFSHRRHHSNTASLERDEVFVPKPKSRMPWYFKHLTNPPARLLILFLTL 181

Query: 188 ILGWPMYLAFNSSGRFYDRFTSHYDPNSPIFSQKERLQVQISNAGVVTVWYLLYKLAVAK 247
            LGWPMYLAFNSSGRFYDRFTSHY PNSPIFS KE LQV IS+AGVV  WYLLYKLA A+
Sbjct: 182 TLGWPMYLAFNSSGRFYDRFTSHYYPNSPIFSGKEWLQVHISSAGVVAFWYLLYKLAAAR 241

Query: 248 GITWVICMYLLPLTVMNAFVVLISSLQHTHPSLPYYDTSEWDWLRGNLVTVDRDYGMILN 307
           GI WVI +YLLPLTVM AFVVLISSLQHTHPSLPYYD+SEWDWLRGNLVTVDRDYG ILN
Sbjct: 242 GIAWVIRIYLLPLTVMYAFVVLISSLQHTHPSLPYYDSSEWDWLRGNLVTVDRDYG-ILN 301

Query: 308 KMFHNITNTHVIHHLFPSMPHYNAMEATKAVKQMLGEYYQFDETPILKAVWREFRECVYV 367
           ++FHNIT+THVIHHLFPSMPHYNAMEAT+AVK++LGEYY FD TPI KA WREFREC+YV
Sbjct: 302 RVFHNITDTHVIHHLFPSMPHYNAMEATRAVKEVLGEYYHFDGTPIFKAAWREFRECIYV 361

Query: 368 EADDEETKDGDSEASSSKGVFWFRNKL 395
           E D++E    +  AS SKGVFWFRN L
Sbjct: 362 EPDEDE----EGSASCSKGVFWFRNNL 382

BLAST of Lsi10G010090 vs. NCBI nr
Match: Q84UC0.1 (RecName: Full=Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase; Short=TkFAC; AltName: Full=Delta(12)-oleate desaturase; AltName: Full=Linoleoyl-lipid Delta(12) conjugase (11E,13Z-forming) [Trichosanthes kirilowii] >AAO37751.1 fatty acid conjugase [Trichosanthes kirilowii])

HSP 1 Score: 643.7 bits (1659), Expect = 1.1e-180
Identity = 303/394 (76.90%), Postives = 345/394 (87.56%), Query Frame = 0

Query: 1   MGGGEETEMRKQSRGRSNLALGKRITHAKPPFTITQIKKAIPSHCFQRSLYRSFSYIILD 60
           MGGGE  E+R  S   + LA G+RITHAKPPF+I+QIKKAIP HCFQRSLYRSFSY+I D
Sbjct: 1   MGGGEGIEVRSGS-SSTKLAFGERITHAKPPFSISQIKKAIPPHCFQRSLYRSFSYVIFD 60

Query: 61  SILLTTFYYIATTYFHSLPHPIHYLAWPLYWLSQGCVITGLWVIAHECGHHAFSNYQFLD 120
            I  +TFY+IA T FH LPHP+HYLAWPLYW  QG V TGLWVIAHECGH AFS+YQ +D
Sbjct: 61  FIFASTFYHIAATNFHRLPHPLHYLAWPLYWFCQGSVFTGLWVIAHECGHRAFSDYQLVD 120

Query: 121 DLLGFLLHSSFLIPYFSFKISHRRHHANTASLDRDEVFVPTPNSKIPWYFNHLTNPPARL 180
           D++GFLLH+SFLIPYFSFKISHRRHH+NTASL+RDEVFVP P +K+PWYF HLTNPPAR+
Sbjct: 121 DVVGFLLHTSFLIPYFSFKISHRRHHSNTASLERDEVFVPKPKAKMPWYFKHLTNPPARV 180

Query: 181 LIIFLTLILGWPMYLAFNSSGRFYDRFTSHYDPNSPIFSQKERLQVQISNAGVVTVWYLL 240
           LIIF+TL LGWPMYLAFN SGRFY+RFTSH+DPNSPIFS+ E LQV ISNAG+V VWYLL
Sbjct: 181 LIIFITLTLGWPMYLAFNISGRFYERFTSHFDPNSPIFSENEWLQVHISNAGIVAVWYLL 240

Query: 241 YKLAVAKGITWVICMYLLPLTVMNAFVVLISSLQHTHPSLPYYDTSEWDWLRGNLVTVDR 300
           YKLA AKGI WVI MY++P+T+MNAFVVLI+SLQHTHPS PYYD++EW+WLRGNLVT+DR
Sbjct: 241 YKLAAAKGIAWVIRMYVVPVTIMNAFVVLITSLQHTHPSFPYYDSTEWNWLRGNLVTLDR 300

Query: 301 DYGMILNKMFHNITNTHVIHHLFPSMPHYNAMEATKAVKQMLGEYYQFDETPILKAVWRE 360
           DYG ILNK+FHNIT+THV+HHLFPSMPHYNAMEAT+AVKQ+LGEYY FD TPI KA WRE
Sbjct: 301 DYG-ILNKVFHNITDTHVVHHLFPSMPHYNAMEATRAVKQVLGEYYHFDGTPIFKAAWRE 360

Query: 361 FRECVYVEADDEETKDGDSEASSSKGVFWFRNKL 395
           FREC+YVE D++E       +SSSKGVFWFRNKL
Sbjct: 361 FRECIYVEPDNDE-----GASSSSKGVFWFRNKL 387

BLAST of Lsi10G010090 vs. TAIR 10
Match: AT3G12120.1 (fatty acid desaturase 2 )

HSP 1 Score: 493.8 bits (1270), Expect = 1.3e-139
Identity = 232/394 (58.88%), Postives = 301/394 (76.40%), Query Frame = 0

Query: 1   MGGGEETEMRKQSRGRSNLALGKRITHAKPPFTITQIKKAIPSHCFQRSLYRSFSYIILD 60
           MG G    +   S+ +S     KR+   KPPF++  +KKAIP HCF+RS+ RSFSY+I D
Sbjct: 1   MGAGGRMPVPTSSK-KSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISD 60

Query: 61  SILLTTFYYIATTYFHSLPHPIHYLAWPLYWLSQGCVITGLWVIAHECGHHAFSNYQFLD 120
            I+ + FYY+AT YF  LP P+ YLAWPLYW  QGCV+TG+WVIAHECGHHAFS+YQ+LD
Sbjct: 61  IIIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLD 120

Query: 121 DLLGFLLHSSFLIPYFSFKISHRRHHANTASLDRDEVFVPTPNSKIPWYFNHLTNPPARL 180
           D +G + HS  L+PYFS+K SHRRHH+NT SL+RDEVFVP   S I WY  +L NP  R+
Sbjct: 121 DTVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRI 180

Query: 181 LIIFLTLILGWPMYLAFNSSGRFYDRFTSHYDPNSPIFSQKERLQVQISNAGVVTVWYLL 240
           +++ +  +LGWP+YLAFN SGR YD F  H+ PN+PI++ +ERLQ+ +S+AG++ V + L
Sbjct: 181 MMLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGL 240

Query: 241 YKLAVAKGITWVICMYLLPLTVMNAFVVLISSLQHTHPSLPYYDTSEWDWLRGNLVTVDR 300
           Y+ A A+G+  +IC+Y +PL ++NAF+VLI+ LQHTHPSLP+YD+SEWDWLRG L TVDR
Sbjct: 241 YRYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDR 300

Query: 301 DYGMILNKMFHNITNTHVIHHLFPSMPHYNAMEATKAVKQMLGEYYQFDETPILKAVWRE 360
           DYG ILNK+FHNIT+THV HHLF +MPHYNAMEATKA+K +LG+YYQFD TP   A++RE
Sbjct: 301 DYG-ILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYRE 360

Query: 361 FRECVYVEADDEETKDGDSEASSSKGVFWFRNKL 395
            +EC+YVE D    ++GD      KGV+W+ NKL
Sbjct: 361 AKECIYVEPD----REGD-----KKGVYWYNNKL 383

BLAST of Lsi10G010090 vs. TAIR 10
Match: AT3G12120.2 (fatty acid desaturase 2 )

HSP 1 Score: 493.8 bits (1270), Expect = 1.3e-139
Identity = 232/394 (58.88%), Postives = 301/394 (76.40%), Query Frame = 0

Query: 1   MGGGEETEMRKQSRGRSNLALGKRITHAKPPFTITQIKKAIPSHCFQRSLYRSFSYIILD 60
           MG G    +   S+ +S     KR+   KPPF++  +KKAIP HCF+RS+ RSFSY+I D
Sbjct: 1   MGAGGRMPVPTSSK-KSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISD 60

Query: 61  SILLTTFYYIATTYFHSLPHPIHYLAWPLYWLSQGCVITGLWVIAHECGHHAFSNYQFLD 120
            I+ + FYY+AT YF  LP P+ YLAWPLYW  QGCV+TG+WVIAHECGHHAFS+YQ+LD
Sbjct: 61  IIIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLD 120

Query: 121 DLLGFLLHSSFLIPYFSFKISHRRHHANTASLDRDEVFVPTPNSKIPWYFNHLTNPPARL 180
           D +G + HS  L+PYFS+K SHRRHH+NT SL+RDEVFVP   S I WY  +L NP  R+
Sbjct: 121 DTVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRI 180

Query: 181 LIIFLTLILGWPMYLAFNSSGRFYDRFTSHYDPNSPIFSQKERLQVQISNAGVVTVWYLL 240
           +++ +  +LGWP+YLAFN SGR YD F  H+ PN+PI++ +ERLQ+ +S+AG++ V + L
Sbjct: 181 MMLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGL 240

Query: 241 YKLAVAKGITWVICMYLLPLTVMNAFVVLISSLQHTHPSLPYYDTSEWDWLRGNLVTVDR 300
           Y+ A A+G+  +IC+Y +PL ++NAF+VLI+ LQHTHPSLP+YD+SEWDWLRG L TVDR
Sbjct: 241 YRYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDR 300

Query: 301 DYGMILNKMFHNITNTHVIHHLFPSMPHYNAMEATKAVKQMLGEYYQFDETPILKAVWRE 360
           DYG ILNK+FHNIT+THV HHLF +MPHYNAMEATKA+K +LG+YYQFD TP   A++RE
Sbjct: 301 DYG-ILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYRE 360

Query: 361 FRECVYVEADDEETKDGDSEASSSKGVFWFRNKL 395
            +EC+YVE D    ++GD      KGV+W+ NKL
Sbjct: 361 AKECIYVEPD----REGD-----KKGVYWYNNKL 383

BLAST of Lsi10G010090 vs. TAIR 10
Match: AT2G29980.1 (fatty acid desaturase 3 )

HSP 1 Score: 211.5 bits (537), Expect = 1.3e-54
Identity = 126/330 (38.18%), Postives = 188/330 (56.97%), Query Frame = 0

Query: 28  AKPPFTITQIKKAIPSHCFQRSLYRSFSYIILDSILLTTFYYIATTYFHSLPHPIHYLAW 87
           A+PPF I  I+ AIP HC+ +S  RS SY++ D I+      IA  Y  S      +  W
Sbjct: 30  AQPPFKIGDIRAAIPKHCWVKSPLRSMSYVVRD-IIAVAALAIAAVYVDS------WFLW 89

Query: 88  PLYWLSQGCVITGLWVIAHECGHHAFSNYQFLDDLLGFLLHSSFLIPYFSFKISHRRHHA 147
           PLYW +QG +   ++V+ H+CGH +FS+   L+ ++G +LHS  L+PY  ++ISHR HH 
Sbjct: 90  PLYWAAQGTLFWAIFVLGHDCGHGSFSDIPLLNSVVGHILHSFILVPYHGWRISHRTHHQ 149

Query: 148 NTASLDRDEVFVPTPN---SKIPWYFNHLTNPPARLLIIFLTL-ILGWPMYLAFNSSGRF 207
           N   ++ DE +VP P     K+P    H T    R+L   + L +L +P+YL + S G  
Sbjct: 150 NHGHVENDESWVPLPERVYKKLP----HST----RMLRYTVPLPMLAYPLYLCYRSPG-- 209

Query: 208 YDRFTSHYDPNSPIFSQKERLQVQISNAGVVTVWYLLYKLAVAKGITWVICMYLLPLTVM 267
             +  SH++P S +F+  ER  +  S      ++  L  L+   G   V+ +Y +P  + 
Sbjct: 210 --KEGSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVFGPLAVLKVYGVPYIIF 269

Query: 268 NAFVVLISSLQH--THPSLPYYDTSEWDWLRGNLVTVDRDYGMILNKMFHNITNTHVIHH 327
             ++  ++ L H      LP+Y   EW +LRG L T+DRDYG I N + H+I  THVIHH
Sbjct: 270 VMWLDAVTYLHHHGHDEKLPWYRGKEWSYLRGGLTTIDRDYG-IFNNIHHDI-GTHVIHH 329

Query: 328 LFPSMPHYNAMEATKAVKQMLGEYYQFDET 352
           LFP +PHY+ ++ATKA K +LG YY+  +T
Sbjct: 330 LFPQIPHYHLVDATKAAKHVLGRYYREPKT 338

BLAST of Lsi10G010090 vs. TAIR 10
Match: AT3G11170.1 (fatty acid desaturase 7 )

HSP 1 Score: 211.1 bits (536), Expect = 1.6e-54
Identity = 127/353 (35.98%), Postives = 194/353 (54.96%), Query Frame = 0

Query: 28  AKPPFTITQIKKAIPSHCFQRSLYRSFSYIILDSILLTTFYYIATTYFHSLPHPIHYLAW 87
           A PPF +  I+ AIP HC+ ++ ++S SY++ D + +         Y +      +++ W
Sbjct: 92  APPPFNLADIRAAIPKHCWVKNPWKSLSYVVRD-VAIVFALAAGAAYLN------NWIVW 151

Query: 88  PLYWLSQGCVITGLWVIAHECGHHAFSNYQFLDDLLGFLLHSSFLIPYFSFKISHRRHHA 147
           PLYWL+QG +   L+V+ H+CGH +FSN   L+ ++G LLHSS L+PY  ++ISHR HH 
Sbjct: 152 PLYWLAQGTMFWALFVLGHDCGHGSFSNDPKLNSVVGHLLHSSILVPYHGWRISHRTHHQ 211

Query: 148 NTASLDRDEVFVPTPNSKIPWYFNHLTNPPARLLIIFLTLILGWPMYLAFNSSGRFYDRF 207
           N   ++ DE + P  + KI   +N L  P          ++L +P YL   S G    + 
Sbjct: 212 NHGHVENDESWHPM-SEKI---YNTLDKPTRFFRFTLPLVMLAYPFYLWARSPG----KK 271

Query: 208 TSHYDPNSPIFSQKERLQVQISNAGVVTVWYLLYKLAVAKGITWVICMYLLPLTVMNAFV 267
            SHY P+S +F  KER  V  S A    +  LL  L    G   ++ +Y +P  +   ++
Sbjct: 272 GSHYHPDSDLFLPKERKDVLTSTACWTAMAALLVCLNFTIGPIQMLKLYGIPYWINVMWL 331

Query: 268 VLISSLQH--THPSLPYYDTSEWDWLRGNLVTVDRDYGMILNKMFHNITNTHVIHHLFPS 327
             ++ L H      LP+Y   EW +LRG L T+DRDYG+I N   H+   THVIHHLFP 
Sbjct: 332 DFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGLINN--IHHDIGTHVIHHLFPQ 391

Query: 328 MPHYNAMEATKAVKQMLGEYYQFDETP------ILKAVWREFRECVYVEADDE 373
           +PHY+ +EAT+A K +LG+YY+  +        +L+ + +  +E  YV  + E
Sbjct: 392 IPHYHLVEATEAAKPVLGKYYREPDKSGPLPLHLLEILAKSIKEDHYVSDEGE 427

BLAST of Lsi10G010090 vs. TAIR 10
Match: AT5G05580.1 (fatty acid desaturase 8 )

HSP 1 Score: 208.8 bits (530), Expect = 8.2e-54
Identity = 121/322 (37.58%), Postives = 178/322 (55.28%), Query Frame = 0

Query: 28  AKPPFTITQIKKAIPSHCFQRSLYRSFSYIILDSILLTTFYYIATTYFHSLPHPIHYLAW 87
           A PPF +  I+ AIP HC+ ++ + S SY++ D  ++     +A  YF+      ++L W
Sbjct: 85  APPPFNLADIRAAIPKHCWVKNPWMSMSYVVRDVAIVFGLAAVA-AYFN------NWLLW 144

Query: 88  PLYWLSQGCVITGLWVIAHECGHHAFSNYQFLDDLLGFLLHSSFLIPYFSFKISHRRHHA 147
           PLYW +QG +   L+V+ H+CGH +FSN   L+ + G LLHSS L+PY  ++ISHR HH 
Sbjct: 145 PLYWFAQGTMFWALFVLGHDCGHGSFSNDPRLNSVAGHLLHSSILVPYHGWRISHRTHHQ 204

Query: 148 NTASLDRDEVFVPTPNSKIPWYFNHLTNPPARLLIIFLTLILGWPMYLAFNSSGRFYDRF 207
           N   ++ DE + P P S     + +L              +L +P YL   S G    + 
Sbjct: 205 NHGHVENDESWHPLPES----IYKNLEKTTQMFRFTLPFPMLAYPFYLWNRSPG----KQ 264

Query: 208 TSHYDPNSPIFSQKERLQVQISNAGVVTVWYLLYKLAVAKGITWVICMYLLPLTVMNAFV 267
            SHY P+S +F  KE+  V  S A    +  LL  L    G   ++ +Y +P  +   ++
Sbjct: 265 GSHYHPDSDLFLPKEKKDVLTSTACWTAMAALLVCLNFVMGPIQMLKLYGIPYWIFVMWL 324

Query: 268 VLISSLQH--THPSLPYYDTSEWDWLRGNLVTVDRDYGMILNKMFHNITNTHVIHHLFPS 327
             ++ L H      LP+Y   EW +LRG L T+DRDYG I N   H+   THVIHHLFP 
Sbjct: 325 DFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGWINN--IHHDIGTHVIHHLFPQ 384

Query: 328 MPHYNAMEATKAVKQMLGEYYQ 348
           +PHY+ +EAT+A K +LG+YY+
Sbjct: 385 IPHYHLVEATEAAKPVLGKYYR 389