Lsi10G009760 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi10G009760
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein
Location	chr10: 14104907 .. 14106765 (-)
RNA-Seq Expression	Lsi10G009760
Synteny	Lsi10G009760

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGCCTCACCTCATTTGCTTGGCCTCTTCTCCTGCGCCATGGCGCTGCTTTTTCTAGGGTTGGTGAGCTCCGACATAGACCAGGACAGGGCGGAGTGCGCGGACCAGGTGGTTGGGCTCGCCACTTGCCTGCCCTACGTGGGCGGCGAAGCCAAGGCACCGACGCCCGACTGCTGCAGTGGTCTCAAGCTTGTGCTTGAAAAGAGTAAGAAATGTCTTTGTGTGTTGATTAAGGACCGTAACGACCCAAGCCTCGGACTCAAAGTTAATCTTACGCTTGCTCTTGGACTTCCCACTGCTTGCCATGACCCTGCCGACATCAAAGAATGCATCGGTATACTTTACTTTTAAAAAAATATTTAGCATAATCTATTATATAGCAAATTTTTTTTAATATTTTAATTTATTGTGGGTATATAAAAAGAGAATTTTTCACCCATAAAAAAAGTGTCAAATTATTTATAAAAAATGACAAAAAAAAAAAGCATCGATAGACTTCCATCAGTATTTATTAGTGATAAATTTCTATCATTTTTATCATTCATAGATCTTAATAGATTTCTATCAGAGTCTATCACAATTATCTAAAAAATTTGTTATTTTGTATAAATAATTTTTCTTATTTTTCTATTTTTAAAAATTCTTCTCTTATTCCTAATATTTTTCTCTTTAATTTATTAATTAATCTGAAGAAATTCATGAGTAGTATATATGTGAATTACAGGGCTTCTCCATCTGCCGCCAAACTCCCAAGAGGCTAAGGATTTTCTGGGGTTGAACACCAAGGAAACTAACGCTACATCAGCACCTGCTACTGGTATAACTCAAAATTCCTTTTTTATTTTTTTTCCCTTCTCTCTTTTAGAATTTTATTTAAATTTATCAACTTTTAAAAAGAAATTAATTAACTCCCTCTCCATATATATGAACTTTCAAATATAATTATGAAAAAAAATTTAAAAATAGCAAAAATTTTAGATAATTATGATAGATGTTGATAGAAGTAGGTCTATCAGTGATAGAAATTATAGAAATATATCATTGATAGATACTGATAGAAGTCTATCAATGTCTATCAGTAATTTTTTTGTTATTTTCTATAAATAATTTGACATTTTTTCTATCTGTGAAAATTTTCCTATAATTATTTTAGTTATTAATGATATTTTCTTTTAAAGATAATTGCAATGGTAGCACTTTTAAAACTAATAATTAATTTGTAAAACAATATTTTTAAAGAATTGCAAATGTAGCAAAATCTATTGATGATAAACTATATCTTTAATAGACTTCTTTTAGCAATATATAATCTATCATTAATATAGACTTCGAGATCTAGGATCTAAATTTTGCTATATCTGTAAATTTTTTTGCAGTGTTATATCTATTAATCTTTTGAGTCATATTGCTCTATTTGCAACTATCCTTTATCTTTTAACTAAGAAATGCTTTAGTTGCAATTGCATGCACTATAAGTACAAATATTGTCGATCTCACATTAAAACAATCAACGGAACTCACTAGATGAGTTACTAAAAAAAATAACATTCTCAAGAAAAAAGTCATTCTTTTGACCCTTAGGAGTAAAAATGACATTTTCTACAAGTGAATTAAATGCTTTTTTTTTTTTTTTAATTTGAAGTGTAAAAATAGATTTTTTTTTTTTTTAAAAAAAAAGAAACTCTAATGCATGGTGAGGAATATTTTGTTTGTTTTAATTTGTTGCAGTTAGTGGGAATTCCACAAAAAAATCTGAAGCGAAAAATGATGGTGGAACGAGGAGGAATCAAGGGTTGGGTGTGGAGATATTCGTTTGGTTTTTCACTTCATCGTTTCTACTCACTTGTATTTGGTAG

mRNA sequence

ATGGCCGCCTCACCTCATTTGCTTGGCCTCTTCTCCTGCGCCATGGCGCTGCTTTTTCTAGGGTTGGTGAGCTCCGACATAGACCAGGACAGGGCGGAGTGCGCGGACCAGGTGGTTGGGCTCGCCACTTGCCTGCCCTACGTGGGCGGCGAAGCCAAGGCACCGACGCCCGACTGCTGCAGTGGTCTCAAGCTTGTGCTTGAAAAGAGTAAGAAATGTCTTTGTGTGTTGATTAAGGACCGTAACGACCCAAGCCTCGGACTCAAAGTTAATCTTACGCTTGCTCTTGGACTTCCCACTGCTTGCCATGACCCTGCCGACATCAAAGAATGCATCGGGCTTCTCCATCTGCCGCCAAACTCCCAAGAGGCTAAGGATTTTCTGGGGTTGAACACCAAGGAAACTAACGCTACATCAGCACCTGCTACTGTTAGTGGGAATTCCACAAAAAAATCTGAAGCGAAAAATGATGGTGGAACGAGGAGGAATCAAGGGTTGGGTGTGGAGATATTCGTTTGGTTTTTCACTTCATCGTTTCTACTCACTTGTATTTGGTAG

Coding sequence (CDS)

ATGGCCGCCTCACCTCATTTGCTTGGCCTCTTCTCCTGCGCCATGGCGCTGCTTTTTCTAGGGTTGGTGAGCTCCGACATAGACCAGGACAGGGCGGAGTGCGCGGACCAGGTGGTTGGGCTCGCCACTTGCCTGCCCTACGTGGGCGGCGAAGCCAAGGCACCGACGCCCGACTGCTGCAGTGGTCTCAAGCTTGTGCTTGAAAAGAGTAAGAAATGTCTTTGTGTGTTGATTAAGGACCGTAACGACCCAAGCCTCGGACTCAAAGTTAATCTTACGCTTGCTCTTGGACTTCCCACTGCTTGCCATGACCCTGCCGACATCAAAGAATGCATCGGGCTTCTCCATCTGCCGCCAAACTCCCAAGAGGCTAAGGATTTTCTGGGGTTGAACACCAAGGAAACTAACGCTACATCAGCACCTGCTACTGTTAGTGGGAATTCCACAAAAAAATCTGAAGCGAAAAATGATGGTGGAACGAGGAGGAATCAAGGGTTGGGTGTGGAGATATTCGTTTGGTTTTTCACTTCATCGTTTCTACTCACTTGTATTTGGTAG

Protein sequence

MAASPHLLGLFSCAMALLFLGLVSSDIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPTACHDPADIKECIGLLHLPPNSQEAKDFLGLNTKETNATSAPATVSGNSTKKSEAKNDGGTRRNQGLGVEIFVWFFTSSFLLTCIW

Homology

[Close]

BLAST of Lsi10G009760 vs. ExPASy Swiss-Prot
Match: O64865 (Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3702 GN=LTPG14 PE=2 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 2.4e-37
Identity = 88/200 (44.00%), Postives = 124/200 (62.00%), Query Frame = 0


Query: 8   LGLFSCAMALLFLGLV---SSDIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLK 67
           + L + A+AL+ + +V   + D  +D+ EC +Q+VG+ATCLPYV G+AK+PTPDCCSGLK
Sbjct: 6   INLMATAIALIVVAMVVAAADDKTKDKEECTEQLVGMATCLPYVQGQAKSPTPDCCSGLK 65

Query: 68  LVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPTACHDPADIKECIGLLHLPPNSQEA 127
            VL  +KKCLCV+I+DRNDP LGL++N++LAL LP+ CH  AD+ +C  LLHL PNS +A
Sbjct: 66  QVLNSNKKCLCVIIQDRNDPDLGLQINVSLALALPSVCHAAADVTKCPALLHLDPNSPDA 125

Query: 128 KDFLGLNTKETNAT--------SAPATVSGNSTKKSEAKNDGGTR-----RN-------Q 185
           + F  L  K  N T        S+P  +S + T  S+  N+ G       RN       Q
Sbjct: 126 QVFYQL-AKGLNKTGPASAPTGSSPGPISISPTSGSDDGNNSGRTTSVPGRNHAQSFYKQ 185

[Close]

BLAST of Lsi10G009760 vs. ExPASy Swiss-Prot
Match: F4I082 (Non-specific lipid transfer protein GPI-anchored 6 OS=Arabidopsis thaliana OX=3702 GN=LTPG6 PE=2 SV=2)

HSP 1 Score: 154.8 bits (390), Expect = 9.3e-37
Identity = 76/164 (46.34%), Postives = 106/164 (64.63%), Query Frame = 0


Query: 18  LFLGLVSSDIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVLEKSKKCLCVL 77
           + LG  +SD+ QDR EC +Q++ L+TC+PYVGG+AKAPT DCC+G   V+ KS+KC+C+L
Sbjct: 17  MLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVIRKSEKCVCIL 76

Query: 78  IKDRNDPSLGLKVNLTLALGLPTACHDPA-DIKECIGLLHLPPNSQEAKDFLGLNTKETN 137
           ++D++DP LG+K+N TLA  LP+ACH  A +I +CI +LHLP NS  AK+F  L   E N
Sbjct: 77  VRDKDDPQLGIKINATLAAHLPSACHITAPNITDCISILHLPRNSTLAKEFENLGRIEDN 136

Query: 138 ATSAPAT---VSGNSTKKSEAKNDGGTRRNQGLGVEIFVWFFTS 178
             S   T     G    K+E     G +    LGVE+ ++   S
Sbjct: 137 YNSTSPTQIHKDGTGGGKAEPVKSNGWKEKSWLGVELLIYLLVS 180

[Close]

BLAST of Lsi10G009760 vs. ExPASy Swiss-Prot
Match: O64864 (Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3702 GN=LTPG13 PE=2 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.7e-35
Identity = 85/198 (42.93%), Postives = 119/198 (60.10%), Query Frame = 0


Query: 10  LFSCAMALLFLGLV----SSDIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKL 69
           + + A+AL+ + +V     +D  +D+ EC  Q+VG+ATCLPYV G+AK+PTPDCCSGLK 
Sbjct: 8   VMATAIALIMVAMVVDAAGADKGKDKEECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQ 67

Query: 70  VLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPTACHDPADIKECIGLLHLPPNSQEAK 129
           V+    KCLC++I++RNDP LGL+VN++LAL LP+ CH  ADI +C  LLHL PNS +A+
Sbjct: 68  VINSDMKCLCMIIQERNDPDLGLQVNVSLALALPSVCHATADITKCPALLHLDPNSPDAQ 127

Query: 130 DF--LGLNTKETNATSAPA-----TVSGNSTKKSEAKNDGGTRRN------------QGL 185
            F  L     ET + SAP        S +ST  S A N+ G   +            Q L
Sbjct: 128 VFYQLAKGLNETVSASAPTGSASEPTSMSSTPGSSAGNNSGRTTSVPGTNHAQSFSKQWL 187

[Close]

BLAST of Lsi10G009760 vs. ExPASy Swiss-Prot
Match: Q9M2G1 (Non-specific lipid transfer protein GPI-anchored 22 OS=Arabidopsis thaliana OX=3702 GN=LTPG22 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.8e-25
Identity = 64/157 (40.76%), Postives = 94/157 (59.87%), Query Frame = 0


Query: 4   SPHLLGLFSCAMALLFLGLVSSDIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGL 63
           +P +L L    +A++FLG V S++ QD   C D +  L +CLP+V  +AKAP   CCS L
Sbjct: 10  NPQMLAL-CITVAVMFLG-VRSELSQDIKGCQDAMSDLYSCLPFVTNKAKAPDSTCCSTL 69

Query: 64  KLVLEK--SKKCLCVLIKDRNDPSLGLKVNLTLALGLPTACHDPADIKECIGLLHLPPNS 123
           K+ ++K  ++KCLC L+KDR+DP LG KV+   A+ LP+ACH PA+I +C  LLHL P+S
Sbjct: 70  KVKIDKGQTRKCLCTLVKDRDDPGLGFKVDANRAMSLPSACHVPANISQCPDLLHLLPDS 129

Query: 124 QEAKDFLGLNTKETNATSAPATVSGNSTKKSEAKNDG 159
             ++ F       +      A  + +S K  + K  G
Sbjct: 130 PASQIFKQFTESSSQTVGHKAVSTSSSIKGRDNKQFG 164

[Close]

BLAST of Lsi10G009760 vs. ExPASy Swiss-Prot
Match: Q9LJ86 (Non-specific lipid transfer protein GPI-anchored 5 OS=Arabidopsis thaliana OX=3702 GN=LTPG5 PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 9.6e-10
Identity = 41/153 (26.80%), Postives = 75/153 (49.02%), Query Frame = 0


Query: 18  LFLGLVSSDIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVLEKSKKCLCVL 77
           +F+ ++SS +   ++ C + ++ ++ CL Y+ G + +P   CC+ L  V++ S  CLC +
Sbjct: 12  VFMAVMSSTMVSAQSSCTNALISMSPCLNYITGNSTSPNQQCCNQLSRVVQSSPDCLCQV 71

Query: 78  IKDRNDPSLGLKVNLTLALGLPTACHDPADIKECIGLLHLPPNSQ-EAKDFLGLNTKETN 137
           + +     LG+ VN T ALGLP AC+           +  PP S+       G +T ++ 
Sbjct: 72  L-NGGGSQLGINVNQTQALGLPRACN-----------VQTPPVSRCNTGGGGGGSTSDSP 131

Query: 138 ATSAPATVSGNSTKKSEAKNDGGTRRNQGLGVE 170
           A S  ++  GN +K        G   + G  ++
Sbjct: 132 AESPNSSGPGNGSKTVPVGEGDGPPSSDGSSIK 152

[Close]

BLAST of Lsi10G009760 vs. ExPASy TrEMBL
Match: A0A0A0KZ48 (AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G280560 PE=4 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 1.1e-69
Identity = 139/176 (78.98%), Postives = 157/176 (89.20%), Query Frame = 0


Query: 14  AMALLFLGLVSSDIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVLEKSKKC 73
           A+ALL +G VSS+IDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVL+KS+KC
Sbjct: 12  ALALLSVGFVSSNIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVLDKSRKC 71

Query: 74  LCVLIKDRNDPSLGLKVNLTLALGLPTACHDPADIKECIGLLHLPPNSQEAKDFLGL-NT 133
           LCVLIKDR+DPSLGLKVNL+LALGLP+ACH PA+IK+C+GLLHL PNS EAKDFLG  N+
Sbjct: 72  LCVLIKDRDDPSLGLKVNLSLALGLPSACHAPANIKDCVGLLHLSPNSPEAKDFLGSPNS 131

Query: 134 KETNATSAPA---TVSGNSTKKSEAKNDGGTRRNQGLGVEIFVWFFTSSFLLTCIW 186
           KETN TS PA    VSG+S++ SE KND GT+RNQ LGVE+F+WF TSSFL TCIW
Sbjct: 132 KETN-TSTPAHATPVSGSSSQNSEVKND-GTKRNQWLGVEMFIWFITSSFLFTCIW 185

[Close]

BLAST of Lsi10G009760 vs. ExPASy TrEMBL
Match: A0A5D3DDD4 (Protein YLS3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00450 PE=4 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.6e-68
Identity = 139/175 (79.43%), Postives = 154/175 (88.00%), Query Frame = 0


Query: 14  AMALLFLGLVSSDIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVLEKSKKC 73
           A+ALL +G VSS+IDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVL+KS+KC
Sbjct: 12  ALALLSVGFVSSNIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVLDKSRKC 71

Query: 74  LCVLIKDRNDPSLGLKVNLTLALGLPTACHDPADIKECIGLLHLPPNSQEAKDFLG-LNT 133
           LCVLIKDR+DPSLGLKVNLTLALGLP+ACH PADIK+CIGLLHL PNS EAKDFLG  N+
Sbjct: 72  LCVLIKDRDDPSLGLKVNLTLALGLPSACHAPADIKDCIGLLHLSPNSPEAKDFLGSSNS 131

Query: 134 KETN-ATSAPAT-VSGNSTKKSEAKNDGGTRRNQGLGVEIFVWFFTSSFLLTCIW 186
           KETN ++SA AT VSGNS++ +E KN G  R NQ LGVEI + F TSSFL TCIW
Sbjct: 132 KETNTSSSAHATPVSGNSSQNTEVKNGGTKRNNQWLGVEILICFITSSFLFTCIW 186

[Close]

BLAST of Lsi10G009760 vs. ExPASy TrEMBL
Match: A0A1S3BMU8 (protein YLS3 OS=Cucumis melo OX=3656 GN=LOC103491579 PE=4 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.6e-68
Identity = 139/175 (79.43%), Postives = 154/175 (88.00%), Query Frame = 0


Query: 14  AMALLFLGLVSSDIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVLEKSKKC 73
           A+ALL +G VSS+IDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVL+KS+KC
Sbjct: 12  ALALLSVGFVSSNIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVLDKSRKC 71

Query: 74  LCVLIKDRNDPSLGLKVNLTLALGLPTACHDPADIKECIGLLHLPPNSQEAKDFLG-LNT 133
           LCVLIKDR+DPSLGLKVNLTLALGLP+ACH PADIK+CIGLLHL PNS EAKDFLG  N+
Sbjct: 72  LCVLIKDRDDPSLGLKVNLTLALGLPSACHAPADIKDCIGLLHLSPNSPEAKDFLGSSNS 131

Query: 134 KETN-ATSAPAT-VSGNSTKKSEAKNDGGTRRNQGLGVEIFVWFFTSSFLLTCIW 186
           KETN ++SA AT VSGNS++ +E KN G  R NQ LGVEI + F TSSFL TCIW
Sbjct: 132 KETNTSSSAHATPVSGNSSQNTEVKNGGTKRNNQWLGVEILICFITSSFLFTCIW 186

[Close]

BLAST of Lsi10G009760 vs. ExPASy TrEMBL
Match: A0A6J1JBX4 (protein YLS3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484364 PE=4 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 5.2e-67
Identity = 133/185 (71.89%), Postives = 154/185 (83.24%), Query Frame = 0


Query: 2   AASPHLLGLFSCAMALLFLGLVSSDIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCS 61
           ++S +   L SCA+ALLF+G VSS++DQDRAECADQVVGLATCLPYVGGEA+APTPDCCS
Sbjct: 5   SSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCS 64

Query: 62  GLKLVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPTACHDPADIKECIGLLHLPPNS 121
           GLK+VLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLP ACH PA+I++CIGLLHL PNS
Sbjct: 65  GLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNS 124

Query: 122 QEAKDFLGLNTKETNATSAPATVS-GNSTKKSEAKNDGGTRRNQGLGVEIFVWFFTSSFL 181
           Q+AKDF+G  +K  N T+APAT + GNST+    K  G   RNQ LG E+F+ F TSS L
Sbjct: 125 QDAKDFMG--SKGANTTAAPATAAPGNSTQTPMEKGSG--MRNQWLGAEMFIAFLTSSLL 184

Query: 182 LTCIW 186
            TC+W
Sbjct: 185 FTCVW 185

[Close]

BLAST of Lsi10G009760 vs. ExPASy TrEMBL
Match: A0A6J1J894 (protein YLS3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484364 PE=4 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 2.6e-66
Identity = 132/184 (71.74%), Postives = 152/184 (82.61%), Query Frame = 0


Query: 2   AASPHLLGLFSCAMALLFLGLVSSDIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCS 61
           ++S +   L SCA+ALLF+G VSS++DQDRAECADQVVGLATCLPYVGGEA+APTPDCCS
Sbjct: 5   SSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCS 64

Query: 62  GLKLVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPTACHDPADIKECIGLLHLPPNS 121
           GLK+VLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLP ACH PA+I++CIGLLHL PNS
Sbjct: 65  GLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNS 124

Query: 122 QEAKDFLGLNTKETNATSAPATVSGNSTKKSEAKNDGGTRRNQGLGVEIFVWFFTSSFLL 181
           Q+AKDF+G  +K  N T+APA   GNST+    K  G   RNQ LG E+F+ F TSS L 
Sbjct: 125 QDAKDFMG--SKGANTTAAPA-APGNSTQTPMEKGSG--MRNQWLGAEMFIAFLTSSLLF 183

Query: 182 TCIW 186
           TC+W
Sbjct: 185 TCVW 183

[Close]

BLAST of Lsi10G009760 vs. NCBI nr
Match: XP_038900291.1 (non-specific lipid transfer protein GPI-anchored 6 [Benincasa hispida])

HSP 1 Score: 300.8 bits (769), Expect = 8.0e-78
Identity = 152/185 (82.16%), Postives = 164/185 (88.65%), Query Frame = 0


Query: 2   AASPHLLGLFSCAMALLFLGLVSSDIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCS 61
           + S HLL      MALLF+G VSS+IDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCS
Sbjct: 3   STSTHLL----IVMALLFVGFVSSNIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCS 62

Query: 62  GLKLVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPTACHDPADIKECIGLLHLPPNS 121
           GL LVL+KS+KCLC+LIKDR+DPSLGLKVNLTLALGLPTACH PADIK+CIGLLHL PNS
Sbjct: 63  GLNLVLQKSRKCLCILIKDRDDPSLGLKVNLTLALGLPTACHTPADIKDCIGLLHLSPNS 122

Query: 122 QEAKDFLGLNTKETNATSAPAT-VSGNSTKKSEAKNDGGTRRNQGLGVEIFVWFFTSSFL 181
           +EAKDFL  N K +N TSAPAT VSGNST+ SEAKNDGGT+RNQ LGVEIFVWF TSSFL
Sbjct: 123 KEAKDFLETNPKGSNTTSAPATAVSGNSTQNSEAKNDGGTKRNQWLGVEIFVWFSTSSFL 182

Query: 182 LTCIW 186
           LTCIW
Sbjct: 183 LTCIW 183

[Close]

BLAST of Lsi10G009760 vs. NCBI nr
Match: XP_011653558.1 (protein YLS3 [Cucumis sativus] >KGN54079.1 hypothetical protein Csa_018100 [Cucumis sativus])

HSP 1 Score: 272.7 bits (696), Expect = 2.3e-69
Identity = 139/176 (78.98%), Postives = 157/176 (89.20%), Query Frame = 0


Query: 14  AMALLFLGLVSSDIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVLEKSKKC 73
           A+ALL +G VSS+IDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVL+KS+KC
Sbjct: 12  ALALLSVGFVSSNIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVLDKSRKC 71

Query: 74  LCVLIKDRNDPSLGLKVNLTLALGLPTACHDPADIKECIGLLHLPPNSQEAKDFLGL-NT 133
           LCVLIKDR+DPSLGLKVNL+LALGLP+ACH PA+IK+C+GLLHL PNS EAKDFLG  N+
Sbjct: 72  LCVLIKDRDDPSLGLKVNLSLALGLPSACHAPANIKDCVGLLHLSPNSPEAKDFLGSPNS 131

Query: 134 KETNATSAPA---TVSGNSTKKSEAKNDGGTRRNQGLGVEIFVWFFTSSFLLTCIW 186
           KETN TS PA    VSG+S++ SE KND GT+RNQ LGVE+F+WF TSSFL TCIW
Sbjct: 132 KETN-TSTPAHATPVSGSSSQNSEVKND-GTKRNQWLGVEMFIWFITSSFLFTCIW 185

[Close]

BLAST of Lsi10G009760 vs. NCBI nr
Match: XP_008449799.1 (PREDICTED: protein YLS3 [Cucumis melo] >KAA0040058.1 protein YLS3 [Cucumis melo var. makuwa] >TYK21681.1 protein YLS3 [Cucumis melo var. makuwa])

HSP 1 Score: 268.9 bits (686), Expect = 3.4e-68
Identity = 139/175 (79.43%), Postives = 154/175 (88.00%), Query Frame = 0


Query: 14  AMALLFLGLVSSDIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVLEKSKKC 73
           A+ALL +G VSS+IDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVL+KS+KC
Sbjct: 12  ALALLSVGFVSSNIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVLDKSRKC 71

Query: 74  LCVLIKDRNDPSLGLKVNLTLALGLPTACHDPADIKECIGLLHLPPNSQEAKDFLG-LNT 133
           LCVLIKDR+DPSLGLKVNLTLALGLP+ACH PADIK+CIGLLHL PNS EAKDFLG  N+
Sbjct: 72  LCVLIKDRDDPSLGLKVNLTLALGLPSACHAPADIKDCIGLLHLSPNSPEAKDFLGSSNS 131

Query: 134 KETN-ATSAPAT-VSGNSTKKSEAKNDGGTRRNQGLGVEIFVWFFTSSFLLTCIW 186
           KETN ++SA AT VSGNS++ +E KN G  R NQ LGVEI + F TSSFL TCIW
Sbjct: 132 KETNTSSSAHATPVSGNSSQNTEVKNGGTKRNNQWLGVEILICFITSSFLFTCIW 186

[Close]

BLAST of Lsi10G009760 vs. NCBI nr
Match: XP_023512874.1 (protein YLS3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 266.9 bits (681), Expect = 1.3e-67
Identity = 136/185 (73.51%), Postives = 154/185 (83.24%), Query Frame = 0


Query: 2   AASPHLLGLFSCAMALLFLGLVSSDIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCS 61
           ++S +   L SCAMALL +G VSS++DQDRAECADQVVGLATCLPYVGGEA+APTPDCCS
Sbjct: 5   SSSTNYTALISCAMALLLVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCS 64

Query: 62  GLKLVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPTACHDPADIKECIGLLHLPPNS 121
           GLK+VLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLP ACH PA+I++CIGLLHL PNS
Sbjct: 65  GLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNS 124

Query: 122 QEAKDFLGLNTKETNATSAPATVS-GNSTKKSEAKNDGGTRRNQGLGVEIFVWFFTSSFL 181
           Q+AKDF+G  TK  N T+APAT + GNST+    K  G   RNQ LGVE+F+ F TSS L
Sbjct: 125 QDAKDFMG--TKGANTTAAPATAAPGNSTQTPVEKGSG--MRNQWLGVEMFIGFLTSSLL 184

Query: 182 LTCIW 186
            TCIW
Sbjct: 185 FTCIW 185

[Close]

BLAST of Lsi10G009760 vs. NCBI nr
Match: XP_022986686.1 (protein YLS3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 263.8 bits (673), Expect = 1.1e-66
Identity = 133/185 (71.89%), Postives = 154/185 (83.24%), Query Frame = 0


Query: 2   AASPHLLGLFSCAMALLFLGLVSSDIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCS 61
           ++S +   L SCA+ALLF+G VSS++DQDRAECADQVVGLATCLPYVGGEA+APTPDCCS
Sbjct: 5   SSSTNYTALISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCS 64

Query: 62  GLKLVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPTACHDPADIKECIGLLHLPPNS 121
           GLK+VLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLP ACH PA+I++CIGLLHL PNS
Sbjct: 65  GLKVVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNS 124

Query: 122 QEAKDFLGLNTKETNATSAPATVS-GNSTKKSEAKNDGGTRRNQGLGVEIFVWFFTSSFL 181
           Q+AKDF+G  +K  N T+APAT + GNST+    K  G   RNQ LG E+F+ F TSS L
Sbjct: 125 QDAKDFMG--SKGANTTAAPATAAPGNSTQTPMEKGSG--MRNQWLGAEMFIAFLTSSLL 184

Query: 182 LTCIW 186
            TC+W
Sbjct: 185 FTCVW 185

[Close]

BLAST of Lsi10G009760 vs. TAIR 10
Match: AT2G44300.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 156.8 bits (395), Expect = 1.7e-38
Identity = 88/200 (44.00%), Postives = 124/200 (62.00%), Query Frame = 0


Query: 8   LGLFSCAMALLFLGLV---SSDIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLK 67
           + L + A+AL+ + +V   + D  +D+ EC +Q+VG+ATCLPYV G+AK+PTPDCCSGLK
Sbjct: 6   INLMATAIALIVVAMVVAAADDKTKDKEECTEQLVGMATCLPYVQGQAKSPTPDCCSGLK 65

Query: 68  LVLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPTACHDPADIKECIGLLHLPPNSQEA 127
            VL  +KKCLCV+I+DRNDP LGL++N++LAL LP+ CH  AD+ +C  LLHL PNS +A
Sbjct: 66  QVLNSNKKCLCVIIQDRNDPDLGLQINVSLALALPSVCHAAADVTKCPALLHLDPNSPDA 125

Query: 128 KDFLGLNTKETNAT--------SAPATVSGNSTKKSEAKNDGGTR-----RN-------Q 185
           + F  L  K  N T        S+P  +S + T  S+  N+ G       RN       Q
Sbjct: 126 QVFYQL-AKGLNKTGPASAPTGSSPGPISISPTSGSDDGNNSGRTTSVPGRNHAQSFYKQ 185

[Close]

BLAST of Lsi10G009760 vs. TAIR 10
Match: AT1G55260.2 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 156.4 bits (394), Expect = 2.3e-38
Identity = 75/161 (46.58%), Postives = 105/161 (65.22%), Query Frame = 0


Query: 18  LFLGLVSSDIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVLEKSKKCLCVL 77
           + LG  +SD+ QDR EC +Q++ L+TC+PYVGG+AKAPT DCC+G   V+ KS+KC+C+L
Sbjct: 60  MLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVIRKSEKCVCIL 119

Query: 78  IKDRNDPSLGLKVNLTLALGLPTACHDPA-DIKECIGLLHLPPNSQEAKDFLGLNTKETN 137
           ++D++DP LG+K+N TLA  LP+ACH  A +I +CI +LHLP NS  AK+F  L   E N
Sbjct: 120 VRDKDDPQLGIKINATLAAHLPSACHITAPNITDCISILHLPRNSTLAKEFENLGRIEDN 179

Query: 138 ATSAPATVSGNSTKKSEAKNDGGTRRNQGLGVEIFVWFFTS 178
             S   T       K+E     G +    LGVE+ ++   S
Sbjct: 180 YNSTSPTQIHKGGGKAEPVKSNGWKEKSWLGVELLIYLLVS 220

[Close]

BLAST of Lsi10G009760 vs. TAIR 10
Match: AT1G55260.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 154.8 bits (390), Expect = 6.6e-38
Identity = 76/164 (46.34%), Postives = 106/164 (64.63%), Query Frame = 0


Query: 18  LFLGLVSSDIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVLEKSKKCLCVL 77
           + LG  +SD+ QDR EC +Q++ L+TC+PYVGG+AKAPT DCC+G   V+ KS+KC+C+L
Sbjct: 60  MLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVIRKSEKCVCIL 119

Query: 78  IKDRNDPSLGLKVNLTLALGLPTACHDPA-DIKECIGLLHLPPNSQEAKDFLGLNTKETN 137
           ++D++DP LG+K+N TLA  LP+ACH  A +I +CI +LHLP NS  AK+F  L   E N
Sbjct: 120 VRDKDDPQLGIKINATLAAHLPSACHITAPNITDCISILHLPRNSTLAKEFENLGRIEDN 179

Query: 138 ATSAPAT---VSGNSTKKSEAKNDGGTRRNQGLGVEIFVWFFTS 178
             S   T     G    K+E     G +    LGVE+ ++   S
Sbjct: 180 YNSTSPTQIHKDGTGGGKAEPVKSNGWKEKSWLGVELLIYLLVS 223

[Close]

BLAST of Lsi10G009760 vs. TAIR 10
Match: AT2G44290.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 150.6 bits (379), Expect = 1.2e-36
Identity = 85/198 (42.93%), Postives = 119/198 (60.10%), Query Frame = 0


Query: 10  LFSCAMALLFLGLV----SSDIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKL 69
           + + A+AL+ + +V     +D  +D+ EC  Q+VG+ATCLPYV G+AK+PTPDCCSGLK 
Sbjct: 8   VMATAIALIMVAMVVDAAGADKGKDKEECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQ 67

Query: 70  VLEKSKKCLCVLIKDRNDPSLGLKVNLTLALGLPTACHDPADIKECIGLLHLPPNSQEAK 129
           V+    KCLC++I++RNDP LGL+VN++LAL LP+ CH  ADI +C  LLHL PNS +A+
Sbjct: 68  VINSDMKCLCMIIQERNDPDLGLQVNVSLALALPSVCHATADITKCPALLHLDPNSPDAQ 127

Query: 130 DF--LGLNTKETNATSAPA-----TVSGNSTKKSEAKNDGGTRRN------------QGL 185
            F  L     ET + SAP        S +ST  S A N+ G   +            Q L
Sbjct: 128 VFYQLAKGLNETVSASAPTGSASEPTSMSSTPGSSAGNNSGRTTSVPGTNHAQSFSKQWL 187

[Close]

BLAST of Lsi10G009760 vs. TAIR 10
Match: AT3G58550.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 116.7 bits (291), Expect = 2.0e-26
Identity = 64/157 (40.76%), Postives = 94/157 (59.87%), Query Frame = 0


Query: 4   SPHLLGLFSCAMALLFLGLVSSDIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGL 63
           +P +L L    +A++FLG V S++ QD   C D +  L +CLP+V  +AKAP   CCS L
Sbjct: 10  NPQMLAL-CITVAVMFLG-VRSELSQDIKGCQDAMSDLYSCLPFVTNKAKAPDSTCCSTL 69

Query: 64  KLVLEK--SKKCLCVLIKDRNDPSLGLKVNLTLALGLPTACHDPADIKECIGLLHLPPNS 123
           K+ ++K  ++KCLC L+KDR+DP LG KV+   A+ LP+ACH PA+I +C  LLHL P+S
Sbjct: 70  KVKIDKGQTRKCLCTLVKDRDDPGLGFKVDANRAMSLPSACHVPANISQCPDLLHLLPDS 129

Query: 124 QEAKDFLGLNTKETNATSAPATVSGNSTKKSEAKNDG 159
             ++ F       +      A  + +S K  + K  G
Sbjct: 130 PASQIFKQFTESSSQTVGHKAVSTSSSIKGRDNKQFG 164

The following BLAST results are available for this feature:

BLAST of Lsi10G009760 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O64865	2.4e-37	44.00	Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3...	[more]
F4I082	9.3e-37	46.34	Non-specific lipid transfer protein GPI-anchored 6 OS=Arabidopsis thaliana OX=37...	[more]
O64864	1.7e-35	42.93	Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3...	[more]
Q9M2G1	2.8e-25	40.76	Non-specific lipid transfer protein GPI-anchored 22 OS=Arabidopsis thaliana OX=3...	[more]
Q9LJ86	9.6e-10	26.80	Non-specific lipid transfer protein GPI-anchored 5 OS=Arabidopsis thaliana OX=37...	[more]

BLAST of Lsi10G009760 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KZ48	1.1e-69	78.98	AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G280560 PE=4 SV...	[more]
A0A5D3DDD4	1.6e-68	79.43	Protein YLS3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00450 P...	[more]
A0A1S3BMU8	1.6e-68	79.43	protein YLS3 OS=Cucumis melo OX=3656 GN=LOC103491579 PE=4 SV=1	[more]
A0A6J1JBX4	5.2e-67	71.89	protein YLS3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484364 PE=4 SV...	[more]
A0A6J1J894	2.6e-66	71.74	protein YLS3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484364 PE=4 SV...	[more]

BLAST of Lsi10G009760 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038900291.1	8.0e-78	82.16	non-specific lipid transfer protein GPI-anchored 6 [Benincasa hispida]	[more]
XP_011653558.1	2.3e-69	78.98	protein YLS3 [Cucumis sativus] >KGN54079.1 hypothetical protein Csa_018100 [Cucu...	[more]
XP_008449799.1	3.4e-68	79.43	PREDICTED: protein YLS3 [Cucumis melo] >KAA0040058.1 protein YLS3 [Cucumis melo ...	[more]
XP_023512874.1	1.3e-67	73.51	protein YLS3-like [Cucurbita pepo subsp. pepo]	[more]
XP_022986686.1	1.1e-66	71.89	protein YLS3-like isoform X1 [Cucurbita maxima]	[more]

BLAST of Lsi10G009760 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44300.1	1.7e-38	44.00	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G55260.2	2.3e-38	46.58	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G55260.1	6.6e-38	46.34	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT2G44290.1	1.2e-36	42.93	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT3G58550.1	2.0e-26	40.76	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	PFAM	PF14368	LTP_2	coord: 28..111 e-value: 1.1E-11 score: 44.7
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	GENE3D	1.10.110.10		coord: 31..108 e-value: 1.3E-13 score: 52.6
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	SUPERFAMILY	47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 33..111
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 138..160
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 138..153
None	No IPR available	PANTHER	PTHR33044:SF194	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN	coord: 10..173
None	No IPR available	CDD	cd00010	AAI_LTSS	coord: 41..103 e-value: 1.6002E-16 score: 68.2222
IPR043325	Alpha-Amylase Inhibitors (AAI), Lipid Transfer (LT) and Seed Storage (SS) Protein	PANTHER	PTHR33044	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED	coord: 10..173

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G009760.1	Lsi10G009760.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane