Lsi10G009150 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi10G009150
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Mediator of RNA polymerase II transcription subunit 21
Location	chr10: 13626756 .. 13631614 (+)
RNA-Seq Expression	Lsi10G009150
Synteny	Lsi10G009150

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGTCGTTGATCTTGCAAAACGATTCTGTGATGGAGCAGCACCCCGTATTATTAATGGATGCCTTAGGACCTTTACAAAGGACATCAAAGAAATTGATTCAACTCATGTCGGAAGCAAGAAGTCCGTGCATAACCCTGAACTTTTGAGGTGCCTCAGGACCTTTGTAAGGATATTGAAGAATTTGACTCAACTCATGTTGTAGCTCAAGTAAGTGATTTGGGGGTACGTTCATTAAACTTGTTGTTGTCATCAGCAGCTAACGTGAAGTGATTTGAAGAATCTCATACTTGCCGCAGATCACAACAATAATTTCTTTCCCTTTCGCCCATGCAGGCGTGGATTTCTGTTATATCATAGAGTTGAGTAATGAGGTGCCTGATGTGTGAGAGAATAAGTTCTCAAATTAATCTAGTACCGGGAGAGATGTTATTATACACATAATATTATCAATATAGTGAAGTTTTAGTAAAGGTGCGATGTGTCGTGGTCATGTTTGTCCAGTGTGCCCCTGGTTGCATGCCTCTAGCAAATTTTAGTTTTAATCCTTTCTACCCGAAGTTGTGTGTTAAGCCGTGTTGTTCCTCTTATATGCTTTGTAGCCTACAACACAACCTCCCAAATAGCGATGAATCAAAAGTGAGGGTCGATTTAGATTGATTTTCTAAGTGTTTAAATAATTGCAAAAAGAAGTGTTTATGAACACTTAGAAAGTCAATGCAAACGGGCCCTCATTTTAATTCATTATAAATATAATAAGTCTATTGCAGTCTATCGTTGATCGATAGTGAAATTTTTCTATATTGGTAAATAGTTTGATATTTTTTTTACTTATAATAATTTTCTTAATAATTACTCTTAAATTTATCTTAGGGGTGTTTGGCTCATCAACATGAGTTGGTAAAAATTAACAACTCAATGTTTGATGTTGGTGGAGTTGAGTTGGTTATTTTTACTAACTCACCTCAATTCGCTCTAACTCAATTCAATTCTTCCTCCTACCGATGTTTTCAATGACCCCACTCGTCGGCCACCACCGACGACCACCAATGCCATACTTCGACGACCAACTTCGATGACTACCTTTGTGCGGTCAACTTCGGGCTCCAGAAACCACCTTTTCAATTCCAGTGATAACCACTTCTGACGACCAACATTGACGTTCACCTCAAGCGACCAACTCCGACAAAGACCACCATTGATGATCAACTGCGATAACCACTTATAGCAGCCAACTTCAACGATCACCTCAGGCGATTAACTCCAACCACCAACTTTGTCGTTTATCTCCAACGACTAACCCTAACAACCAATTCAACCAATCATATACTAAAAGAAAAAAAAATCTGATTGTTGAATCTTATCTATATACAAAATGATATACCAATTCAACATCTAACCACCCCTTCGATTAATCATATTCTACTATTATCAATTGCATTTATTAGATCGAGTAGGTATACATTCGATGAGTCATGTCCCATCTCAAAGAATTGCACTTTGGCTACATGAGGAAAGGGTAGAAAATGAATTTATTAAAAATATATGGGCAAAAGAGAACAAAATATTCTCCTTCATAACTCTATTTATAAAAATTAAACATGTTATAAATTTGAAAATTTGAATTTAAATTATCTTAAAGTGGTAAGTTGTCGGGATGCTTTTACTCGGTAGATAAGATATTAAATTTTGTTCGGTTCAATCTATTGTGACTACCAATTTAATATTCTACAAGTTTTTTTACACCAAAATGTTGTAAAATAATTAGGCAAGATGCACCTAAGCTGACCCGAACCGTCATAAATATAAAAAAAAAAAATTAAATTTTGTTAACATAACAATTATTGGGATAAGACAATTTCAACCTCCTGTTAAAAAAATGTCGATTATTATTAAGCCCTTATTTTGTGATGTGGTGTTCTACAATTAAAAAGAATAGGAAATTTAAAATAAAAAAAAAAAAGAAAAGAAAAAGAAAAAAAGATCATGATTAAAAATTGGAGAAGTAAAAGGAAAAGGAGGACAAAAGGAACCCTAATAGCTCATCTGTTTCCTCTCCCCCTCACGTTCAGCCGGCCAGGCCCTCCCTTGTCTCCGGCAGCAGCGCGACGACCACGGACCCGCGATGAAAATCCTTGGAGATTAACAGCGGATCGCACTCCCACTCAGTCAGTTTCTGGAAGTGGTTTGCTGATTTAATTGCAGTGATATGTGTGTTTGCTGAATCATCTTCTGGACATTTCTTCTTAGGGATAAGGTTGAAGATGGACATAATTTCCCAATTGCAGGAACAAGTTAACGCAATTGCTGCTTTAGCTTTCAACACATTTGGGACATTACAAAGAGACGCTCCTCCAGTTCGTTTATCCCCTAATTACCCTGAACCACCGTCCCACGAACCTACAGAGGACAGTGCAAATATCGCAGAGCAGCCCAAGCTTATGAGTGCCGCTTTGGTGAAAGCTGCTAAACAGGTGTGAATTATTTATTCTGTGGGATTTAAGTGTTTGCTTGATTCTTAATACTTCGGGGTGGGAAATATGAATTTCTGATGCATGTGCCTCTGGTATTAACTTCTTCACGGGTTGAAACATCAAGAAAATTATTTGCCTTTTCCTTTTGGGGAAAATTTATGTTGCTATTTTTGTTTTCAAATGTCAGTATCCTAAGATTAATTTTATATGCATTCTGTCTACTGGATAAGTAACCGAGAATCTACTATCTACTTGAAGCACCCTTCTGTTGAGGTTGGAAGAGAGTAATATCAAGGCAGCAGATTTCCTGCTACTTGTTTGAGATACATACACATTCTAATGCTATGATTTATGATACAATCCATCAAAATGAGCAAATTTGGTATTGGAAACTTCAATCTATTTTTGCCATCCAACGTCTGATACTACTTAGGTAGCTATAGTGTTTTGTATCGGCAATAAAAGAAAGTACTGTGAATTTTTGTAGTTATTCTTTGCTTTTAAGTTTTAACTGCCTGTTATGTATATTTAGCCCCACTTTAAGGAGCTCTTATGTGTGTTGATATTTTAGAATTCACTATTCAAACCCTGTATTCGAGCAATTTTGCTGAGAGAATTTTGATTTTCATAAGTTTTCAGCAACGGAGTTGAATGTAGCAATGAATTAAGGTTTGTTCTTTCGAACAATTTTTTTAAGGACTATATAGAATTTATAAAATTAACTGAGTGTATCCACATTACATTTGGGAATCCCAGTGTGATAGTAAGGTAATAGAAACGTGTAAAAGTTTTCCAATTCTGGTGAACTTTTAATGTCTTCTAGATGTGTTTCTACAAGGAGCCAGATTTGTTCTGTACATTATCTCATTTTCTGTATTTTGTATGCTCACCTCTCGATTGGTATATTATTTATTAGCTTTCTTAGGAGTTGCAGGGAGTCGACTGAGGAGTTTCTCCTCCGTCCACCTTCTTCTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTCTGTGTGTGTGTTGGAGAAAATGATGTTTTTGTGGTAGGTTGGAGTGTGTGCTATTTTGTGGGGTTTGTGGGCCGAGAGAAACAATATGATCTTTAAGGGTAGAAATAATAGGATCTTCAACGATAGAAATAATAGAATCTTGGAGATTCTTTGTTTCTCTTTGGTGTCAATTTTTTGAAATTACTCGCTTAGTCACATTCACTTGATTAGAACCCCTTCTTATCGTTAGCTTCCCTTTTTGTGGGCATAACCTTTTTGCATGTCAGTGTATTCTTTCATCTTTTTCCAATAAAAGTCTAGTTTTTACTTCAACAACAAAATTTATTAGCATTTTGGACAAAAGATAGGGTTGGGACCTTGCTTCATATATATTCAATCCAATTGAACATGTGTACCTTTTTAAAAAAATTGTTGTTATTATTTTCTGGCACAGTTTGATGCATTGGTTGCAGCATTGCCTCTATCTGATGGAGGTGAAGAAGAGCAGTTGAAAAGGATTGCAGAACTACAGGTATGAATCTTCTCATATTCCGATGTTATAAGACCAAGAATTCTTGTGTTGTTTACTTCTCAGTACGTTGGAAACTCGTTATCCATTCCAGGCTGAGAACGATGCTGTAGGTCAAGAACTTCAAAAGCAGCTACAAGCAGCAGGTGTTCTCTCGTCTTCTTGCTAATCTTTATATTTTCAACATCCCTAGATGCAATTATGAGTCCATCATGACTTCGAATGCATGCCCAAAAATAAGTTCCTATTTTAGATTGTTACTTCTGAATCGTTGTCTGGCTTGCTTGCGACTTACAATGTTCAAATAATTTAAGCCCTAAATTTCTCATGTTCACTCCGTTCTATTGAGAATATACTGATATTTTGCCACAGCTGCACAGCTAACTATCTCATTGAAACAATCTGTTGCAGAGAAAGAATTAATACAAGTTCAGGAGCTGTTCAACCAAGTGGCAGATAATTGCTTGAACTTGAAGAAAGCAGCTTGAAAGAGTGGGGGAATTTTCCTATACTACCAATTTATGAAAGTTTTCCATATCTCCCCCATTCAATTTCTGCCACATGGCTCTTTGTTTTTTATATTAAATATATATGTAGAAATATACGTGGCCCTACAGTTTAGGGAAGAAATATATTTCTATCAATGTCTTATTTTAAAAAGAAATAAAAAACTGTTCTCTTCTATTTATTTATTTATGTGCTTATTACTGTTGGAGAAATTATTTTAAATGATAAAACTATAGAAATATTTTTAAATATAGCAAAATGGTACGTTTTCATATGAGGAAACTTTTTATTATTTTGCTATATTTTTAAATAAGGTTGTTATTTTGCCATATATGAAAACATCCCTTATTATT

mRNA sequence

ATGAAGGTCGTTGATCTTGCAAAACGATTCTGTGATGGAGCAGCACCCCGTATTATTAATGGATGCCTTAGGACCTTTACAAAGGACATCAAAGAAATTGATTCAACTCATGTCGGAAGCAAGAAGTCCGTGCATAACCCTGAACTTTTGAGGTGCCTCAGGACCTTTATCACAACAATAATTTCTTTCCCTTTCGCCCATGCAGGCGTGGATTTCTGTTATATCATAGAGTTGAGTAATGAGGTGCCTGATGTCCGGCCAGGCCCTCCCTTGTCTCCGGCAGCAGCGCGACGACCACGGACCCGCGATGAAAATCCTTGGAGATTAACAGCGGATCGCACTCCCACTCAGTCAGTTTCTGGAAGTGGGATAAGGTTGAAGATGGACATAATTTCCCAATTGCAGGAACAAGTTAACGCAATTGCTGCTTTAGCTTTCAACACATTTGGGACATTACAAAGAGACGCTCCTCCAGTTCGTTTATCCCCTAATTACCCTGAACCACCGTCCCACGAACCTACAGAGGACAGTGCAAATATCGCAGAGCAGCCCAAGCTTATGAGTGCCGCTTTGGTGAAAGCTGCTAAACAGTTTGATGCATTGGTTGCAGCATTGCCTCTATCTGATGGAGGTGAAGAAGAGCAGTTGAAAAGGATTGCAGAACTACAGGCTGAGAACGATGCTGTAGGTCAAGAACTTCAAAAGCAGCTACAAGCAGCAGAGAAAGAATTAATACAAGTTCAGGAGCTGTTCAACCAAGTGGCAGATAATTGCTTGAACTTGAAGAAAGCAGCTTGAAAGAGTGGGGGAATTTTCCTATACTACCAATTTATGAAAGTTTTCCATATCTCCCCCATTCAATTTCTGCCACATGGCTCTTTGTTTTTTATATTAAATATATATGTAGAAATATACGTGGCCCTACAGTTTAGGGAAGAAATATATTTCTATCAATGTCTTATTTTAAAAAGAAATAAAAAACTGTTCTCTTCTATTTATTTATTTATGTGCTTATTACTGTTGGAGAAATTATTTTAAATGATAAAACTATAGAAATATTTTTAAATATAGCAAAATGGTACGTTTTCATATGAGGAAACTTTTTATTATTTTGCTATATTTTTAAATAAGGTTGTTATTTTGCCATATATGAAAACATCCCTTATTATT

Coding sequence (CDS)

ATGAAGGTCGTTGATCTTGCAAAACGATTCTGTGATGGAGCAGCACCCCGTATTATTAATGGATGCCTTAGGACCTTTACAAAGGACATCAAAGAAATTGATTCAACTCATGTCGGAAGCAAGAAGTCCGTGCATAACCCTGAACTTTTGAGGTGCCTCAGGACCTTTATCACAACAATAATTTCTTTCCCTTTCGCCCATGCAGGCGTGGATTTCTGTTATATCATAGAGTTGAGTAATGAGGTGCCTGATGTCCGGCCAGGCCCTCCCTTGTCTCCGGCAGCAGCGCGACGACCACGGACCCGCGATGAAAATCCTTGGAGATTAACAGCGGATCGCACTCCCACTCAGTCAGTTTCTGGAAGTGGGATAAGGTTGAAGATGGACATAATTTCCCAATTGCAGGAACAAGTTAACGCAATTGCTGCTTTAGCTTTCAACACATTTGGGACATTACAAAGAGACGCTCCTCCAGTTCGTTTATCCCCTAATTACCCTGAACCACCGTCCCACGAACCTACAGAGGACAGTGCAAATATCGCAGAGCAGCCCAAGCTTATGAGTGCCGCTTTGGTGAAAGCTGCTAAACAGTTTGATGCATTGGTTGCAGCATTGCCTCTATCTGATGGAGGTGAAGAAGAGCAGTTGAAAAGGATTGCAGAACTACAGGCTGAGAACGATGCTGTAGGTCAAGAACTTCAAAAGCAGCTACAAGCAGCAGAGAAAGAATTAATACAAGTTCAGGAGCTGTTCAACCAAGTGGCAGATAATTGCTTGAACTTGAAGAAAGCAGCTTGA

Protein sequence

MKVVDLAKRFCDGAAPRIINGCLRTFTKDIKEIDSTHVGSKKSVHNPELLRCLRTFITTIISFPFAHAGVDFCYIIELSNEVPDVRPGPPLSPAAARRPRTRDENPWRLTADRTPTQSVSGSGIRLKMDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLMSAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQVQELFNQVADNCLNLKKAA

Homology

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BLAST of Lsi10G009150 vs. ExPASy Swiss-Prot
Match: C0LU16 (Mediator of RNA polymerase II transcription subunit 21 OS=Arabidopsis thaliana OX=3702 GN=MED21 PE=1 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 1.8e-49
Identity = 108/137 (78.83%), Postives = 117/137 (85.40%), Query Frame = 0


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEP-TEDSANIAEQPKL 187
           MDIISQLQEQVN IAA+ FN FGTLQRDAPPV+LSPNYPEPP+    T+D+    EQPK 
Sbjct: 1   MDIISQLQEQVNTIAAITFNAFGTLQRDAPPVQLSPNYPEPPATTTVTDDATPFPEQPKQ 60

Query: 188 MSAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQ 247
           +SA LVKAAKQFDALVAALPLS+GGE  QLKRIAELQ END VGQELQKQL+AAEKEL Q
Sbjct: 61  LSAGLVKAAKQFDALVAALPLSEGGEGAQLKRIAELQVENDLVGQELQKQLEAAEKELKQ 120

Query: 248 VQELFNQVADNCLNLKK 264
           VQELF Q ADNCLN+KK
Sbjct: 121 VQELFGQAADNCLNMKK 137

[Close]

BLAST of Lsi10G009150 vs. ExPASy Swiss-Prot
Match: Q7ZTI5 (Mediator of RNA polymerase II transcription subunit 21 OS=Danio rerio OX=7955 GN=med21 PE=2 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 6.6e-04
Identity = 38/142 (26.76%), Postives = 69/142 (48.59%), Query Frame = 0


Query: 129 DIISQLQEQVNAIAALAFNTFGTLQRDAPP-----VRLSPNYPEPPSHEPTEDSANIAEQ 188
           D ++QLQ+ VN++A    N  G LQ+ APP     ++ + N  +P +  PTE+ A     
Sbjct: 3   DRLTQLQDAVNSLADQFCNAIGVLQQCAPPASFSNIQTAINKDQPSN--PTEEYA----- 62

Query: 189 PKLMSAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKE 248
            +L +A + + AK  D L+ +LP  +     Q   + +L+ EN      L++ +   +  
Sbjct: 63  -QLFAALIARTAKDVDVLIDSLPSEESTAALQAASLRQLEEENQEAAARLEEVVYRGDAL 122

Query: 249 LIQVQELFNQVADNCLNLKKAA 266
           L ++Q     +A + L  +  A
Sbjct: 123 LEKIQTALADIAQSQLRTRSGA 136

[Close]

BLAST of Lsi10G009150 vs. ExPASy TrEMBL
Match: A0A0A0KZ00 (Mediator of RNA polymerase II transcription subunit 21 OS=Cucumis sativus OX=3659 GN=Csa_4G268030 PE=3 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 8.0e-61
Identity = 130/137 (94.89%), Postives = 133/137 (97.08%), Query Frame = 0


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 187
           MDIISQLQEQVN IAALAFNTFGTLQRDAPPVRLSPNYPEPPS EPTED+A IAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60

Query: 188 SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 247
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAEND VGQELQKQLQAAEKEL+QV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120

Query: 248 QELFNQVADNCLNLKKA 265
           QELF+QVADNCLNLKKA
Sbjct: 121 QELFSQVADNCLNLKKA 137

[Close]

BLAST of Lsi10G009150 vs. ExPASy TrEMBL
Match: A0A6J1K432 (Mediator of RNA polymerase II transcription subunit 21 OS=Cucurbita maxima OX=3661 GN=LOC111491504 PE=3 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 1.0e-60
Identity = 130/137 (94.89%), Postives = 132/137 (96.35%), Query Frame = 0


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 187
           MDIISQLQEQVN IAALAFNTFGTLQRDAPPVRLSPNYPEPP HEPTEDSAN AEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60

Query: 188 SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 247
           SAALVKAAKQFD LVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQL+AAEKEL QV
Sbjct: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 248 QELFNQVADNCLNLKKA 265
           QELF+QVADNCLNLKKA
Sbjct: 121 QELFSQVADNCLNLKKA 137

[Close]

BLAST of Lsi10G009150 vs. ExPASy TrEMBL
Match: A0A6J1H2M0 (Mediator of RNA polymerase II transcription subunit 21 OS=Cucurbita moschata OX=3662 GN=LOC111459801 PE=3 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 1.0e-60
Identity = 130/137 (94.89%), Postives = 132/137 (96.35%), Query Frame = 0


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 187
           MDIISQLQEQVN IAALAFNTFGTLQRDAPPVRLSPNYPEPP HEPTEDSAN AEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60

Query: 188 SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 247
           SAALVKAAKQFD LVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQL+AAEKEL QV
Sbjct: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 248 QELFNQVADNCLNLKKA 265
           QELF+QVADNCLNLKKA
Sbjct: 121 QELFSQVADNCLNLKKA 137

[Close]

BLAST of Lsi10G009150 vs. ExPASy TrEMBL
Match: A0A5A7TEZ9 (Mediator of RNA polymerase II transcription subunit 21 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00920 PE=3 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 1.0e-60
Identity = 130/137 (94.89%), Postives = 133/137 (97.08%), Query Frame = 0


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 187
           MDIISQLQEQVN IAALAFNTFGTLQRDAPPVRLSPNYPEPPS EPTEDSA IAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDSATIAEQPKLM 60

Query: 188 SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 247
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAEND VGQELQKQLQAAE+EL+QV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEQELVQV 120

Query: 248 QELFNQVADNCLNLKKA 265
           QELF+QVADNCLNLKKA
Sbjct: 121 QELFSQVADNCLNLKKA 137

[Close]

BLAST of Lsi10G009150 vs. ExPASy TrEMBL
Match: A0A1S3BN38 (Mediator of RNA polymerase II transcription subunit 21 OS=Cucumis melo OX=3656 GN=LOC103491637 PE=3 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 1.0e-60
Identity = 130/137 (94.89%), Postives = 133/137 (97.08%), Query Frame = 0


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 187
           MDIISQLQEQVN IAALAFNTFGTLQRDAPPVRLSPNYPEPPS EPTEDSA IAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDSATIAEQPKLM 60

Query: 188 SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 247
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAEND VGQELQKQLQAAE+EL+QV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEQELVQV 120

Query: 248 QELFNQVADNCLNLKKA 265
           QELF+QVADNCLNLKKA
Sbjct: 121 QELFSQVADNCLNLKKA 137

[Close]

BLAST of Lsi10G009150 vs. NCBI nr
Match: XP_038893979.1 (mediator of RNA polymerase II transcription subunit 21-like [Benincasa hispida] >XP_038893980.1 mediator of RNA polymerase II transcription subunit 21-like [Benincasa hispida])

HSP 1 Score: 252.7 bits (644), Expect = 3.6e-63
Identity = 135/137 (98.54%), Postives = 137/137 (100.00%), Query Frame = 0


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 187
           MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM
Sbjct: 1   MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 60

Query: 188 SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 247
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAE+ELIQV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAERELIQV 120

Query: 248 QELFNQVADNCLNLKKA 265
           QELF+QVADNCLNLKKA
Sbjct: 121 QELFSQVADNCLNLKKA 137

[Close]

BLAST of Lsi10G009150 vs. NCBI nr
Match: XP_038902781.1 (mediator of RNA polymerase II transcription subunit 21-like [Benincasa hispida])

HSP 1 Score: 249.6 bits (636), Expect = 3.0e-62
Identity = 134/137 (97.81%), Postives = 136/137 (99.27%), Query Frame = 0


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 187
           MDIISQLQEQVN+IAALAFNTFGTLQRDAPPVRLSPNYPEPPS EPTEDSANIAEQPKLM
Sbjct: 1   MDIISQLQEQVNSIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDSANIAEQPKLM 60

Query: 188 SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 247
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 120

Query: 248 QELFNQVADNCLNLKKA 265
           QELF+QVADNCLNLKKA
Sbjct: 121 QELFSQVADNCLNLKKA 137

[Close]

BLAST of Lsi10G009150 vs. NCBI nr
Match: XP_004149640.1 (mediator of RNA polymerase II transcription subunit 21 [Cucumis sativus] >KGN54019.1 hypothetical protein Csa_021664 [Cucumis sativus])

HSP 1 Score: 243.8 bits (621), Expect = 1.7e-60
Identity = 130/137 (94.89%), Postives = 133/137 (97.08%), Query Frame = 0


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 187
           MDIISQLQEQVN IAALAFNTFGTLQRDAPPVRLSPNYPEPPS EPTED+A IAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDNATIAEQPKLM 60

Query: 188 SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 247
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAEND VGQELQKQLQAAEKEL+QV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEKELVQV 120

Query: 248 QELFNQVADNCLNLKKA 265
           QELF+QVADNCLNLKKA
Sbjct: 121 QELFSQVADNCLNLKKA 137

[Close]

BLAST of Lsi10G009150 vs. NCBI nr
Match: XP_008449895.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Cucumis melo] >KAA0040117.1 mediator of RNA polymerase II transcription subunit 21 [Cucumis melo var. makuwa])

HSP 1 Score: 243.4 bits (620), Expect = 2.2e-60
Identity = 130/137 (94.89%), Postives = 133/137 (97.08%), Query Frame = 0


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 187
           MDIISQLQEQVN IAALAFNTFGTLQRDAPPVRLSPNYPEPPS EPTEDSA IAEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPSQEPTEDSATIAEQPKLM 60

Query: 188 SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 247
           SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAEND VGQELQKQLQAAE+EL+QV
Sbjct: 61  SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDVVGQELQKQLQAAEQELVQV 120

Query: 248 QELFNQVADNCLNLKKA 265
           QELF+QVADNCLNLKKA
Sbjct: 121 QELFSQVADNCLNLKKA 137

[Close]

BLAST of Lsi10G009150 vs. NCBI nr
Match: XP_022958635.1 (mediator of RNA polymerase II transcription subunit 21 [Cucurbita moschata] >XP_022958636.1 mediator of RNA polymerase II transcription subunit 21 [Cucurbita moschata] >XP_022996209.1 mediator of RNA polymerase II transcription subunit 21 [Cucurbita maxima] >XP_022996211.1 mediator of RNA polymerase II transcription subunit 21 [Cucurbita maxima] >KAG7035787.1 Mediator of RNA polymerase II transcription subunit 21 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 243.4 bits (620), Expect = 2.2e-60
Identity = 130/137 (94.89%), Postives = 132/137 (96.35%), Query Frame = 0


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEPTEDSANIAEQPKLM 187
           MDIISQLQEQVN IAALAFNTFGTLQRDAPPVRLSPNYPEPP HEPTEDSAN AEQPKLM
Sbjct: 1   MDIISQLQEQVNTIAALAFNTFGTLQRDAPPVRLSPNYPEPPPHEPTEDSANFAEQPKLM 60

Query: 188 SAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQV 247
           SAALVKAAKQFD LVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQL+AAEKEL QV
Sbjct: 61  SAALVKAAKQFDVLVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLEAAEKELKQV 120

Query: 248 QELFNQVADNCLNLKKA 265
           QELF+QVADNCLNLKKA
Sbjct: 121 QELFSQVADNCLNLKKA 137

[Close]

BLAST of Lsi10G009150 vs. TAIR 10
Match: AT4G04780.1 (mediator 21 )

HSP 1 Score: 197.6 bits (501), Expect = 1.3e-50
Identity = 108/137 (78.83%), Postives = 117/137 (85.40%), Query Frame = 0


Query: 128 MDIISQLQEQVNAIAALAFNTFGTLQRDAPPVRLSPNYPEPPSHEP-TEDSANIAEQPKL 187
           MDIISQLQEQVN IAA+ FN FGTLQRDAPPV+LSPNYPEPP+    T+D+    EQPK 
Sbjct: 1   MDIISQLQEQVNTIAAITFNAFGTLQRDAPPVQLSPNYPEPPATTTVTDDATPFPEQPKQ 60

Query: 188 MSAALVKAAKQFDALVAALPLSDGGEEEQLKRIAELQAENDAVGQELQKQLQAAEKELIQ 247
           +SA LVKAAKQFDALVAALPLS+GGE  QLKRIAELQ END VGQELQKQL+AAEKEL Q
Sbjct: 61  LSAGLVKAAKQFDALVAALPLSEGGEGAQLKRIAELQVENDLVGQELQKQLEAAEKELKQ 120

Query: 248 VQELFNQVADNCLNLKK 264
           VQELF Q ADNCLN+KK
Sbjct: 121 VQELFGQAADNCLNMKK 137

[Close]

BLAST of Lsi10G009150 vs. TAIR 10
Match: AT4G26370.1 (antitermination NusB domain-containing protein )

HSP 1 Score: 57.0 bits (136), Expect = 2.7e-08
Identity = 24/28 (85.71%), Postives = 26/28 (92.86%), Query Frame = 0


Query: 2   KVVDLAKRFCDGAAPRIINGCLRTFTKD 30
           + VDLAKRFCDG+APRIINGCLRTF KD
Sbjct: 256 EAVDLAKRFCDGSAPRIINGCLRTFVKD 283

The following BLAST results are available for this feature:

BLAST of Lsi10G009150 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C0LU16	1.8e-49	78.83	Mediator of RNA polymerase II transcription subunit 21 OS=Arabidopsis thaliana O...	[more]
Q7ZTI5	6.6e-04	26.76	Mediator of RNA polymerase II transcription subunit 21 OS=Danio rerio OX=7955 GN...	[more]

BLAST of Lsi10G009150 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KZ00	8.0e-61	94.89	Mediator of RNA polymerase II transcription subunit 21 OS=Cucumis sativus OX=365...	[more]
A0A6J1K432	1.0e-60	94.89	Mediator of RNA polymerase II transcription subunit 21 OS=Cucurbita maxima OX=36...	[more]
A0A6J1H2M0	1.0e-60	94.89	Mediator of RNA polymerase II transcription subunit 21 OS=Cucurbita moschata OX=...	[more]
A0A5A7TEZ9	1.0e-60	94.89	Mediator of RNA polymerase II transcription subunit 21 OS=Cucumis melo var. maku...	[more]
A0A1S3BN38	1.0e-60	94.89	Mediator of RNA polymerase II transcription subunit 21 OS=Cucumis melo OX=3656 G...	[more]

BLAST of Lsi10G009150 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893979.1	3.6e-63	98.54	mediator of RNA polymerase II transcription subunit 21-like [Benincasa hispida] ...	[more]
XP_038902781.1	3.0e-62	97.81	mediator of RNA polymerase II transcription subunit 21-like [Benincasa hispida]	[more]
XP_004149640.1	1.7e-60	94.89	mediator of RNA polymerase II transcription subunit 21 [Cucumis sativus] >KGN540...	[more]
XP_008449895.1	2.2e-60	94.89	PREDICTED: mediator of RNA polymerase II transcription subunit 21 [Cucumis melo]...	[more]
XP_022958635.1	2.2e-60	94.89	mediator of RNA polymerase II transcription subunit 21 [Cucurbita moschata] >XP_...	[more]

BLAST of Lsi10G009150 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G04780.1	1.3e-50	78.83	mediator 21	[more]
AT4G26370.1	2.7e-08	85.71	antitermination NusB domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 212..257
None	No IPR available	GENE3D	6.10.280.10	Mediator complex, subunit Med21	coord: 128..257 e-value: 1.1E-27 score: 98.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 86..120
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 156..177
IPR021384	Mediator complex, subunit Med21	PFAM	PF11221	Med21	coord: 128..255 e-value: 1.8E-26 score: 93.1
IPR021384	Mediator complex, subunit Med21	PANTHER	PTHR13381	RNA POLYMERASE II HOLOENZYME COMPONENT SRB7	coord: 128..263
IPR037212	Mediator complex, subunit Med7/Med21-like	SUPERFAMILY	140718	Mediator hinge subcomplex-like	coord: 128..254

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G009150.1	Lsi10G009150.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050832	defense response to fungus
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016592	mediator complex
cellular_component	GO:0043078	polar nucleus
molecular_function	GO:0003712	transcription coregulator activity