Lsi10G008980 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G008980
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionMannan endo-1,4-beta-mannosidase
Locationchr10: 13299348 .. 13303684 (-)
RNA-Seq ExpressionLsi10G008980
SyntenyLsi10G008980
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCAGAACAAGTTTTTTGAGATCCACTGTAGTGGTAAGATGATCTTTGTCTGTATATTAAAATAAAATTATGGGGTTTGAAAGTTTTTAACCAGAAAATGGCAAAAAAAAGGGAAGAGAAGTAATTTAATTTCAGCTGGTTTTGTTGAAATTTTGGAAGAAGAAAAAAAGCCCATTTACGTGGGTTGAACAAGACTGTTGCTTTTTGGGGTCGTGAGAGAAAAGATGGAAAGAAAACAAGAGGGTTTGCAGATCTAATTAAACCATCGAAAGATACCAACATTCCACCATACTCCCTCAACCTCTTTTTTCTTCTTTTGCTTAATCTCTCATTAATTTTTCTTCCTTGCTTTTTTGGGCTACAAGTTTTGGTGTGATTTCTTCAATCTTATAAATTCATCAAAACCCAAGATTGCAATTAGCTTCAAGTGGGACTGTTATTAGTATTCAATCCCCACGAAGAATCGTTGGCGTACATTTGTGGTAGAAATAGGGATCTGGGTATATCATAAGATCAGCCAATTTTAGCGACATTCTTATTCTTTGTGGTTTATTGTTGTAAAATCGCATCTGGGTATGAGCGGTTCTTTGATTTTCTTTGATAGATTGCTATAAACATTGAGTTTTTCTTACAATTGTTCCGATTTGTGTTTTGGGTTTTCCATCGCCGCCAACCAATATTGTGTTTGTAGTGATGGCGGGGAATGGGCTGTTTTACCCGATTCTGGGATTCGCATCTTGTTTGGCTTTCATTTACATGTCGTTTGGGGACTTTAGGATTGGGGGGGAAACTAAAATCCCCATAGTGGATTTTGTAGAGAGGAATGGAACGCAGTTCATGTTAAATGGGAAGGCGTTTTATATAAATGGATGGAATTCTTATTGGTTTATGGACCATGCCGTTGAGGAATATAGAAAGCCTAGAATCAGAGCTATGCTGCAGGCGGCGAGGAAGATGGGGCTTACAGTTTGCCGGACTTGGGCTTTCAACGATGGTGGTTACAATGCCCTTCAGGTTTCTCCCGGCAGGTTTAATGAACGAGTCTTTAAGGTACTAATTTTTCCTCTTTTAGCTGACATTTATGATTGGATTCTTCATTTGTTCAACTCTTGTTTGATTCTACTCTCAATGCTCTGCTTCTGTGTTTTTAATTGGTTTCTTTTTCTGAAATTTTATGGCAATGTCTGTTTAAGAGAGCAATGAGGGTTCAAAGGTTCCATTGGCCAAAGAATGCAAAGCTTGGATGCTTCCACCGACAGAAGAACTCTGTCGGTAGTCGTAAAACAATATGGAATGACTTTATAAATTACATAATTTTTCTTGGACAAGGAACAGTAGTTCTGTTCTTGCCATTATAGTTGAGATGAATAAATTAGACTGAGGACAATAAATTATCTGTCAGTTCCCTGTTTGTTACTGGTTAACTTTGGCATTTAAAAGTTGGATTAATTGAGTATGATTTGAATTCTCACGTTCTGTAACCCCATTAAGAAGCATTATAACTTGTATTTCTGTGAAAGATCAATCTGGTTTTGTATGTTTACTTTACAATATAGTTTCAAGTTCATAAAAGGAGGACTGGAATTCATGGAAGAACCAGATAACTTTCTATTACCATGTCTCTCTTTCTCTTTCTCTATTTGAGAAACAAGGAAAATGAATCATTATCCAAACTATACACAGAAATATTTGTATGTATATATATGTTTGCCATAACAATTTGCTTGGTTTGATGATATTTTCGATTTGCTCAAATGTCTATGTCAGTTGTGTTGTTATCAGCTGAAGTTTTAACTGTTTCTTCTTGGTGGCAGGCTTTGGATCATGTCATTGCAGAATCAAGGCAGCATGGGATAAGACTTCTCCTTAGCTTAGTCAATAATTTGCAAGCATACGGTGGGAAGACTCAGTATGTAAAGTGGGCATGGCAAGATGGTGTTGGTTTAAGCTCTTCCAATGATTCCTTCTTCTATGATCCGTCGATACGTATTTATTTTAAAAATTATATCAAGGTACATTCCATATTCATCCTGAGCTATGCTGTGGATTCTGAGTTTATTGATCCAATAACTATTTGTGATCGTATGAAGCATTAGTTCTCAAACAAAAGTTCTTAACCACGTCTTTGCTGATCTTACATTGTTACCTTATGGTGGTTCTAATGTGCACTGTATTTTTCAGACTGTCCTGACAAGAAAGAACTCTATCACTGGAATTGAATATAGAGATGATCCCACCATCTTTGGATGGGAATTGATTAATGAGCCACGTTGCATGACCGATGCTTCGGGCGATACACTTCAAGTAAGTTTTTTAAGATTTTGATTCTTCTGATTTCTGAATGTTAAACCAAATGAGAATTGAAACTAATGGACTCTGTTCAAATCTTCATACTCCTCAATTAGTTGTAAGATTTTGATACTTTTGCTTCTGAATGTTAAACCAAATGAAAGCTGAACCTCAATGAAACTTACTGTATTGTACTCCAGTTTGAGTCTTTTGATAGCTCAGTTTTGATTTTTGCTTGGGATTCTATACTTAACTTTTCTTTCTTCCAATCTTTTCCTTGTGAAAGTTATGAGCTTCCTGCATGGTATTTCTCAACCTCAACTTTGCTTCAAGTTGATTTCTTTATAGGAAAATCACGTTTAACTTTCACTATGTTTTAAATGACTTTTACCAGAGATAACTCAAGGTTTTAAAACGAACCTACCAGCCCCAATGTTGAATGATATATCTTTATCAGTAAATGGCAGAAGCATTATTCACAATTTGACTCATTTTGGCCTTGTGTTTGATCCTTATATTTGTTTCTCAAGATTCTTGTAGAGATGGCCCAATCCTGTAGGCGTTAATGCGGAATGACCCATTCCTAAAAGACCAATAAAAACGAATTTTCTACTGATGTCACATAGCACTATTGCATTAGAGACGTCCCTTCCATTGCGAAATAGTTGGAGCCCTAACACACGATATTCATTTCAACAGCGATGTGTTTTTTTAAATCAACAGTCGTCCAATCAGCAACGATTAAAAAAAAATGTCACGCTTGAAATGAACATTGCATTATGGCTCTAACTATTTTGCAATGGACAAGACGTCCCTATCGTGAGAAGGAATTCCGAGTGCTATGTGACGTCAGCAAAATGTTAGTTTTCATTGGTTTTTTAGGATTGAGTCATTTCGTATTAATACCTCTAGGATTTGGTCATCCGTACAAAAATCTCTCCTCCCCTTAAAAAAAAAAAAAGAGAGAGAGATTGTTTCTATAACTACGCATAAGCTTATTTTGAGGAATACAAATGTAGGAATGGATTGAAGAAATGACAGCTTACATTAAATCGATTGACAAGAAACATCTACTGACTGTTGGGTTGGAAGGATTTTATGGTCCAAATAGCCGCAAGAAGTCTACTGTGAACCCAGAAGAGTGGGCTTCAAGACTTGGATCTGATTTCATCCGCAATTCTGAGATCCCATACGTAGATTTTGCCTCTGTTCATATCTACCCCGACCATTGGTAAGCATTGATGAACCCCAGTTGAAAACTTGGATGGCAGTAATCAATGATTCTAGTCATCTCTATGCTCTTAAACCTGTTATTTATATAACCAAATTAACATTCTGTGTCTATTAGGTTCCATGATCAAGATTTTGAAGATGAACTGAGATTTGTCTCCAAGTGGATGCTCTCTCATATTGAAGATGGTGACATAGAGATGAAGAAGCCAGTCATGTTTACTGAATTTGGACTGTCAGACCTGAACAAGGGTTTCACTCCAGCTCAACGAGATAGATTTTACAAAACTGTTTACGACGTCATTTACAGATCTGCCAGGAAGAAGCGATCGGGAGCTGGCTCTTTAGCATGGCAGTTCCTTGTTGAAGGAATGGAAGAGAGTAACGATGATTTTGGGATCGTCCCGTGGGAAAGACCATCAATATATCAGCTTATTATAGAGCAATCATGCAGGTTGGCAAGAATTGGTGGAGACATCCAACAGCTAAAAGCATTGAAGTATGTGTGTTCTCAGAGATGGTGATCTGACTATATCTTATTTGAGAGAATTCAATTTGTTCACTTGCTGCTATCTTTTGCCTCTGTGCCCTTTGTTTCTTCTGTTGATACATATTCAGAATAGATTAGTTAGTTTAACATATAGTATTGAGTTTCACATGAACTGGTACAAGCTGTAATGTTTTTTGAGCAAATATATAAGTCATAAATCTATTTTATTTTACCTTTCAAGTCCTCTCCAAAGCTGCAGCATGCAAATCTTTGATCAAATTTATTAGTCCATAATATGAGTGTCTTGCTTCTTGGCAACCCTCTCTAG

mRNA sequence

TTCAGAACAAGTTTTTTGAGATCCACTGTAGTGGTAAGATGATCTTTGTCTGTATATTAAAATAAAATTATGGGGTTTGAAAGTTTTTAACCAGAAAATGGCAAAAAAAAGGGAAGAGAAGTAATTTAATTTCAGCTGGTTTTGTTGAAATTTTGGAAGAAGAAAAAAAGCCCATTTACGTGGGTTGAACAAGACTGTTGCTTTTTGGGGTCGTGAGAGAAAAGATGGAAAGAAAACAAGAGGGTTTGCAGATCTAATTAAACCATCGAAAGATACCAACATTCCACCATACTCCCTCAACCTCTTTTTTCTTCTTTTGCTTAATCTCTCATTAATTTTTCTTCCTTGCTTTTTTGGGCTACAAGTTTTGGTGTGATTTCTTCAATCTTATAAATTCATCAAAACCCAAGATTGCAATTAGCTTCAAGTGGGACTGTTATTAGTATTCAATCCCCACGAAGAATCGTTGGCGTACATTTGTGGTAGAAATAGGGATCTGGGTATATCATAAGATCAGCCAATTTTAGCGACATTCTTATTCTTTGTGGTTTATTGTTGTAAAATCGCATCTGGGTATGAGCGGTTCTTTGATTTTCTTTGATAGATTGCTATAAACATTGAGTTTTTCTTACAATTGTTCCGATTTGTGTTTTGGGTTTTCCATCGCCGCCAACCAATATTGTGTTTGTAGTGATGGCGGGGAATGGGCTGTTTTACCCGATTCTGGGATTCGCATCTTGTTTGGCTTTCATTTACATGTCGTTTGGGGACTTTAGGATTGGGGGGGAAACTAAAATCCCCATAGTGGATTTTGTAGAGAGGAATGGAACGCAGTTCATGTTAAATGGGAAGGCGTTTTATATAAATGGATGGAATTCTTATTGGTTTATGGACCATGCCGTTGAGGAATATAGAAAGCCTAGAATCAGAGCTATGCTGCAGGCGGCGAGGAAGATGGGGCTTACAGTTTGCCGGACTTGGGCTTTCAACGATGGTGGTTACAATGCCCTTCAGGTTTCTCCCGGCAGGTTTAATGAACGAGTCTTTAAGGCTTTGGATCATGTCATTGCAGAATCAAGGCAGCATGGGATAAGACTTCTCCTTAGCTTAGTCAATAATTTGCAAGCATACGGTGGGAAGACTCAGTATGTAAAGTGGGCATGGCAAGATGGTGTTGGTTTAAGCTCTTCCAATGATTCCTTCTTCTATGATCCGTCGATACGTATTTATTTTAAAAATTATATCAAGACTGTCCTGACAAGAAAGAACTCTATCACTGGAATTGAATATAGAGATGATCCCACCATCTTTGGATGGGAATTGATTAATGAGCCACGTTGCATGACCGATGCTTCGGGCGATACACTTCAAGAATGGATTGAAGAAATGACAGCTTACATTAAATCGATTGACAAGAAACATCTACTGACTGTTGGGTTGGAAGGATTTTATGGTCCAAATAGCCGCAAGAAGTCTACTGTGAACCCAGAAGAGTGGGCTTCAAGACTTGGATCTGATTTCATCCGCAATTCTGAGATCCCATACGTAGATTTTGCCTCTGTTCATATCTACCCCGACCATTGGTTCCATGATCAAGATTTTGAAGATGAACTGAGATTTGTCTCCAAGTGGATGCTCTCTCATATTGAAGATGGTGACATAGAGATGAAGAAGCCAGTCATGTTTACTGAATTTGGACTGTCAGACCTGAACAAGGGTTTCACTCCAGCTCAACGAGATAGATTTTACAAAACTGTTTACGACGTCATTTACAGATCTGCCAGGAAGAAGCGATCGGGAGCTGGCTCTTTAGCATGGCAGTTCCTTGTTGAAGGAATGGAAGAGAGTAACGATGATTTTGGGATCGTCCCGTGGGAAAGACCATCAATATATCAGCTTATTATAGAGCAATCATGCAGGTTGGCAAGAATTGGTGGAGACATCCAACAGCTAAAAGCATTGAAGTATGTGTGTTCTCAGAGATGGTGATCTGACTATATCTTATTTGAGAGAATTCAATTTGTTCACTTGCTGCTATCTTTTGCCTCTGTGCCCTTTGTTTCTTCTGTTGATACATATTCAGAATAGATTAGTTAGTTTAACATATAGTATTGAGTTTCACATGAACTGGTACAAGCTGTAATGTTTTTTGAGCAAATATATAAGTCATAAATCTATTTTATTTTACCTTTCAAGTCCTCTCCAAAGCTGCAGCATGCAAATCTTTGATCAAATTTATTAGTCCATAATATGAGTGTCTTGCTTCTTGGCAACCCTCTCTAG

Coding sequence (CDS)

ATGGCGGGGAATGGGCTGTTTTACCCGATTCTGGGATTCGCATCTTGTTTGGCTTTCATTTACATGTCGTTTGGGGACTTTAGGATTGGGGGGGAAACTAAAATCCCCATAGTGGATTTTGTAGAGAGGAATGGAACGCAGTTCATGTTAAATGGGAAGGCGTTTTATATAAATGGATGGAATTCTTATTGGTTTATGGACCATGCCGTTGAGGAATATAGAAAGCCTAGAATCAGAGCTATGCTGCAGGCGGCGAGGAAGATGGGGCTTACAGTTTGCCGGACTTGGGCTTTCAACGATGGTGGTTACAATGCCCTTCAGGTTTCTCCCGGCAGGTTTAATGAACGAGTCTTTAAGGCTTTGGATCATGTCATTGCAGAATCAAGGCAGCATGGGATAAGACTTCTCCTTAGCTTAGTCAATAATTTGCAAGCATACGGTGGGAAGACTCAGTATGTAAAGTGGGCATGGCAAGATGGTGTTGGTTTAAGCTCTTCCAATGATTCCTTCTTCTATGATCCGTCGATACGTATTTATTTTAAAAATTATATCAAGACTGTCCTGACAAGAAAGAACTCTATCACTGGAATTGAATATAGAGATGATCCCACCATCTTTGGATGGGAATTGATTAATGAGCCACGTTGCATGACCGATGCTTCGGGCGATACACTTCAAGAATGGATTGAAGAAATGACAGCTTACATTAAATCGATTGACAAGAAACATCTACTGACTGTTGGGTTGGAAGGATTTTATGGTCCAAATAGCCGCAAGAAGTCTACTGTGAACCCAGAAGAGTGGGCTTCAAGACTTGGATCTGATTTCATCCGCAATTCTGAGATCCCATACGTAGATTTTGCCTCTGTTCATATCTACCCCGACCATTGGTTCCATGATCAAGATTTTGAAGATGAACTGAGATTTGTCTCCAAGTGGATGCTCTCTCATATTGAAGATGGTGACATAGAGATGAAGAAGCCAGTCATGTTTACTGAATTTGGACTGTCAGACCTGAACAAGGGTTTCACTCCAGCTCAACGAGATAGATTTTACAAAACTGTTTACGACGTCATTTACAGATCTGCCAGGAAGAAGCGATCGGGAGCTGGCTCTTTAGCATGGCAGTTCCTTGTTGAAGGAATGGAAGAGAGTAACGATGATTTTGGGATCGTCCCGTGGGAAAGACCATCAATATATCAGCTTATTATAGAGCAATCATGCAGGTTGGCAAGAATTGGTGGAGACATCCAACAGCTAAAAGCATTGAAGTATGTGTGTTCTCAGAGATGGTGA

Protein sequence

MAGNGLFYPILGFASCLAFIYMSFGDFRIGGETKIPIVDFVERNGTQFMLNGKAFYINGWNSYWFMDHAVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHDQDFEDELRFVSKWMLSHIEDGDIEMKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYRSARKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYQLIIEQSCRLARIGGDIQQLKALKYVCSQRW
Homology
BLAST of Lsi10G008980 vs. ExPASy Swiss-Prot
Match: Q7Y223 (Mannan endo-1,4-beta-mannosidase 2 OS=Arabidopsis thaliana OX=3702 GN=MAN2 PE=2 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 2.2e-198
Identity = 318/428 (74.30%), Postives = 373/428 (87.15%), Query Frame = 0

Query: 3   GNGLFYPILGFASCLAFIYMSFGDFRIGGETKIPIVDFVERNGTQFMLNGKAFYINGWNS 62
           GNG   PILGF +C+AFIY+SFGD   G +T+  +  FV+RNGTQF+++GKA Y+NGWNS
Sbjct: 6   GNGPVIPILGFLTCVAFIYLSFGDLWFGLKTEGELA-FVKRNGTQFVVDGKALYVNGWNS 65

Query: 63  YWFMDHAVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKALD 122
           YWFMDHAV ++ + R+ AML+A  KMGLTVCRTWAFNDGGYNALQ+SPGRF+ERVFKALD
Sbjct: 66  YWFMDHAVNDHSRHRVSAMLEAGAKMGLTVCRTWAFNDGGYNALQISPGRFDERVFKALD 125

Query: 123 HVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKN 182
           HVIAE++ HG+RLLLSLVNNLQAYGGKTQYV WAWQ+GVGLSSSNDSFF+DPSIR YFKN
Sbjct: 126 HVIAEAKTHGVRLLLSLVNNLQAYGGKTQYVNWAWQEGVGLSSSNDSFFFDPSIRRYFKN 185

Query: 183 YIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSIDKK 242
           Y+  +LTRKNS+TGIEYR+DPTIF WELINEPRCM+D SGDTLQ+WI EMTA+IKSID K
Sbjct: 186 YLTVLLTRKNSLTGIEYRNDPTIFAWELINEPRCMSDVSGDTLQDWINEMTAFIKSIDNK 245

Query: 243 HLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHDQD 302
           HLLTVGLEGFYGP+S KK TVNPE WAS LGSDF+RNS+ P +DFASVHIYPDHWFHDQ 
Sbjct: 246 HLLTVGLEGFYGPSSPKKLTVNPERWASELGSDFVRNSDSPNIDFASVHIYPDHWFHDQG 305

Query: 303 FEDELRFVSKWMLSHIEDGDIEMKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYRS 362
           FE++L+FV KWMLSHIEDGD E+KKPV+FTEFGLS+LNK + P+QRDRFY+T++DVIY+S
Sbjct: 306 FEEKLKFVVKWMLSHIEDGDKELKKPVLFTEFGLSNLNKDYDPSQRDRFYRTIFDVIYKS 365

Query: 363 ARKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYQLIIEQSCRLARIGGDIQQLKA 422
           A++KRSGAG+L WQFL+EGME  NDDFGIVPWE+ SI +L+IEQSCRL+RI G     K 
Sbjct: 366 AKRKRSGAGTLVWQFLIEGMEGFNDDFGIVPWEQDSIQRLMIEQSCRLSRITGRHLLDKK 425

Query: 423 LKYVCSQR 431
              +CS R
Sbjct: 426 SIEMCSHR 432

BLAST of Lsi10G008980 vs. ExPASy Swiss-Prot
Match: Q9M0H6 (Mannan endo-1,4-beta-mannosidase 5 OS=Arabidopsis thaliana OX=3702 GN=MAN5 PE=2 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 4.0e-184
Identity = 295/421 (70.07%), Postives = 359/421 (85.27%), Query Frame = 0

Query: 10  ILGFASCLAFIYMSFGDFRIGGETKIPIVDFVERNGTQFMLNGKAFYINGWNSYWFMDHA 69
           ILGF  C AFIY+SF D  +  + K  +  FV+RNGTQF+++ K  Y+NGWNSYWFMDHA
Sbjct: 12  ILGFFICAAFIYLSFRDLWLNHKGKAKL-GFVKRNGTQFVVDDKPLYVNGWNSYWFMDHA 71

Query: 70  VEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKALDHVIAESR 129
           V+E+ +  +  ML+A  KMGLTVCRTWAFNDGGYNALQ+SPGRF+ERVF+ALDHVIAE+R
Sbjct: 72  VDEHSRNLVGEMLEAGAKMGLTVCRTWAFNDGGYNALQISPGRFDERVFQALDHVIAEAR 131

Query: 130 QHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKNYIKTVLT 189
           +H +RLLLSLVNNLQAYGGKTQYVKWAWQ+GVGLSSSNDSFF+DPSIR YFKNY+K +LT
Sbjct: 132 KHDVRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFFDPSIRNYFKNYLKVLLT 191

Query: 190 RKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGL 249
           RKNS+TGIEYR+DPTIF WELINEPRC TD SG TLQ+WI+EMT +IKSID KHLLTVGL
Sbjct: 192 RKNSVTGIEYRNDPTIFAWELINEPRCTTDVSGKTLQDWIDEMTGFIKSIDDKHLLTVGL 251

Query: 250 EGFYGPNSRKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHDQDFEDELRF 309
           EGFYGPNS K  TVNPE+WAS+LG+DF++NS    +DFASVHIYPDHWFH+Q FE++L+F
Sbjct: 252 EGFYGPNSPKGLTVNPEQWASQLGTDFVQNSNSSNIDFASVHIYPDHWFHNQTFEEKLKF 311

Query: 310 VSKWMLSHIEDGDIEMKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYRSARKKRSG 369
           V KWM SHIEDG  E+KKPV+FTEFGLS+ NK + P+QRD+FY+ ++DV+Y+SA++K+SG
Sbjct: 312 VVKWMQSHIEDGLKELKKPVLFTEFGLSNQNKDYEPSQRDKFYRIIFDVVYKSAKRKKSG 371

Query: 370 AGSLAWQFLVEGMEESNDDFGIVPWERPSIYQLIIEQSCRLARIGGDIQQLKALKYVCSQ 429
           AG+L WQ  +EGME  NDDFGIVP E+ SIY+L+IEQSCRL ++ G +++ K LK +CS 
Sbjct: 372 AGTLVWQLFMEGMETFNDDFGIVPHEQDSIYKLMIEQSCRLGKVTGRLKEQK-LKELCSH 430

Query: 430 R 431
           R
Sbjct: 432 R 430

BLAST of Lsi10G008980 vs. ExPASy Swiss-Prot
Match: Q0JJD4 (Mannan endo-1,4-beta-mannosidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN2 PE=2 SV=2)

HSP 1 Score: 548.5 bits (1412), Expect = 6.7e-155
Identity = 258/442 (58.37%), Postives = 332/442 (75.11%), Query Frame = 0

Query: 3   GNGL-FYPILGFASCLAFIYMSFGDFRI----------GGETKIPI--VDFVERNGTQFM 62
           GNGL  Y ILG ASC+A +Y S G+  +          GG ++     + FVER G +  
Sbjct: 4   GNGLILYHILGLASCIALVYFSLGEVDLRDALPSLPFSGGASRAAAASLPFVERRGKRLF 63

Query: 63  LNGKAFYINGWNSYWFMDHAVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVS 122
           L+G+ FYINGWNSYW MD AVE   +PR+ +M + A  MGLTVCRTWAFNDG YNALQ+S
Sbjct: 64  LDGRPFYINGWNSYWLMDLAVEPNTRPRVSSMFRTAVSMGLTVCRTWAFNDGSYNALQLS 123

Query: 123 PGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDS 182
           PG F+ERVFKALD V+AE+ +HG+RL+LSL NNL AYGGK QYV+WAW++GVGL++SNDS
Sbjct: 124 PGHFDERVFKALDRVVAEASEHGVRLILSLANNLDAYGGKRQYVRWAWEEGVGLTASNDS 183

Query: 183 FFYDPSIRIYFKNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWI 242
           FF+DP+IR YFK Y+KT+L RKN +TG+EYRDDPTI  WEL+NEPRC +D SGDTLQ W+
Sbjct: 184 FFFDPAIRDYFKVYLKTLLMRKNHLTGLEYRDDPTILAWELMNEPRCTSDPSGDTLQRWM 243

Query: 243 EEMTAYIKSIDKKHLLTVGLEGFYGP-NSRKKSTVNPEEW-ASRLGSDFIRNSEIPYVDF 302
           EEM+AY+KSIDKKHLLTVG EGFYGP +S++K  +NP EW  +  G+DFIRNS+I  +DF
Sbjct: 244 EEMSAYVKSIDKKHLLTVGTEGFYGPTSSQEKLNINPGEWFPNNYGADFIRNSKIQDIDF 303

Query: 303 ASVHIYPDHWFHDQDFEDELRFVSKWMLSHIEDGDIEMKKPVMFTEFGLSDLNKGFTPAQ 362
           ASVH+YPD+W      +++L+F+++W+ +H+EDGD E++KPV+ TEFGLS   +GF  A 
Sbjct: 304 ASVHVYPDNWLQHASLDEKLKFMTRWITAHVEDGDGELEKPVLVTEFGLSHQVEGFEDAH 363

Query: 363 RDRFYKTVYDVIYRSARKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYQLIIEQS 422
           RD  Y+ VYD+++ SAR+  +  G+L WQ   EGMEE +D F IVP ERPS+ +LI EQS
Sbjct: 364 RDVLYRAVYDIVHGSARRGGAAGGALVWQLAAEGMEEYHDGFSIVPSERPSMMRLIKEQS 423

Query: 423 CRLARIG-GDIQQLKALKYVCS 429
           CRLA +  G+    K LK VC+
Sbjct: 424 CRLAAVRYGEEGARKVLKTVCA 445

BLAST of Lsi10G008980 vs. ExPASy Swiss-Prot
Match: Q0DCM5 (Mannan endo-1,4-beta-mannosidase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN6 PE=2 SV=2)

HSP 1 Score: 422.9 bits (1086), Expect = 4.2e-117
Identity = 209/428 (48.83%), Postives = 283/428 (66.12%), Query Frame = 0

Query: 8   YPILGFASCLAFIYMSF-------GDFRIGGETKIPI------VDFVERNGTQFM--LNG 67
           Y +LG    LA +Y+++       G    GG  K+P+      + F  R GT F+    G
Sbjct: 8   YSLLGLLLLLAVVYLTWFPTTHDGGGGGGGGWVKLPVPWLQPRMPFAARRGTHFVDADTG 67

Query: 68  KAFYINGWNSYWFMDHAVEEYRKPRIRA-MLQAARKMGLTVCRTWAFNDGGYNALQVSPG 127
              Y+NGWNSYW +       R P + A ML+  R+MGL+VCRTWAF+DGG  ALQ+SPG
Sbjct: 68  SPLYVNGWNSYWLL-----PARSPALAAEMLRRGRRMGLSVCRTWAFSDGGPGALQISPG 127

Query: 128 RFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFF 187
           RF+E VF+ LD+VI E+R++ IRL+L LVNNL   GGK QYV+WA   G  +++S DSF+
Sbjct: 128 RFSEAVFQVLDYVIYEARRNHIRLILCLVNNLDNLGGKAQYVQWAQAAGANMTNSTDSFY 187

Query: 188 YDPSIRIYFKNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEE 247
             P+I+ Y+K+Y+K +LTR+NS + I Y D+P IF WEL+NEPRC++++SG  LQ WI E
Sbjct: 188 SHPTIKRYYKDYVKAILTRRNSYSRIRYSDEPAIFAWELMNEPRCVSNSSGPYLQAWIAE 247

Query: 248 MTAYIKSIDKKHLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVH 307
           M AY+KS+D  HL+TVG EGFYGP   ++  VNP EWA+ L SDFI+NS + ++DFASVH
Sbjct: 248 MAAYVKSLDTNHLVTVGTEGFYGPGIAERLGVNPGEWAASLCSDFIQNSAVEHIDFASVH 307

Query: 308 IYPDHWFHDQDFEDELRFVSKWMLSHIEDGDIEMKKPVMFTEFGL---SDLNKGFTPAQR 367
            YPD W      E+++R++S W+ SH+ D +  +KKPV+FTE G    SD N   T   R
Sbjct: 308 AYPDSWLPRASLEEKVRYLSNWVDSHLNDSEQILKKPVLFTEVGYLQHSDANSNST-VDR 367

Query: 368 DRFYKTVYDVIYRSARKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYQLIIEQSC 417
           D   + VYD IY SARK ++G+G+L WQ +VEG     D+F +V  +RPS Y LI  QSC
Sbjct: 368 DIILRIVYDKIYDSARKLQAGSGALIWQLMVEGTHMYGDNFSVVARDRPSTYSLITNQSC 427

BLAST of Lsi10G008980 vs. ExPASy Swiss-Prot
Match: Q9LZV3 (Mannan endo-1,4-beta-mannosidase 6 OS=Arabidopsis thaliana OX=3702 GN=MAN6 PE=2 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 4.0e-107
Identity = 178/373 (47.72%), Postives = 256/373 (68.63%), Query Frame = 0

Query: 39  DFVERNGTQFMLNGKAFYINGWNSYWFMDHAVEEYRKPRIRAMLQAARKMGLTVCRTWAF 98
           + V+R G QF LNG+ FY+NG+N+YW M  A +   + ++  + Q A  +G+TV RTWAF
Sbjct: 46  EMVQRKGMQFTLNGQPFYVNGFNTYWMMTLAADNSTRGKVTEVFQQASAVGMTVGRTWAF 105

Query: 99  NDGGYNALQVSPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQ 158
           NDG + ALQ SP  ++E VFKALD V++E+R++ IRL+LSLVNN  AYGGK QYVKW   
Sbjct: 106 NDGQWRALQKSPSVYDEEVFKALDFVLSEARKYKIRLILSLVNNWDAYGGKAQYVKWGNA 165

Query: 159 DGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMT 218
            G+ L +S+D FF +P++R +++++++TVL R N+ T I Y++DPTIF WEL+NEPRC +
Sbjct: 166 SGLNL-TSDDDFFTNPTLRNFYQSHVRTVLNRVNTFTNITYKNDPTIFAWELMNEPRCPS 225

Query: 219 DASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDFIR 278
           D SGD LQ WI+EM  ++KS+D KHL+ +GLEGFYGP++  ++  NP  +A+++G+DFIR
Sbjct: 226 DPSGDKLQSWIQEMAVFVKSLDAKHLVEIGLEGFYGPSAPARTRFNPNPYAAQVGTDFIR 285

Query: 279 NSEIPYVDFASVHIYPDHWFHDQDFEDELRFVSKWMLSHIEDGDIEMKKPVMFTEFGLSD 338
           N+++  +DFASVH+YPD W         L F S WM +H+ED ++ +  PV+FTEFG+S 
Sbjct: 286 NNQVLGIDFASVHVYPDSWISPAVSNSFLEFTSSWMQAHVEDAEMYLGMPVLFTEFGVSA 345

Query: 339 LNKGFTPAQRDRFYKTVYDVIYRSARKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPS 398
            + GF  + RD    TVY +   S RK  +GAGSL WQ   +G E  +D + +      +
Sbjct: 346 HDPGFNTSFRDMMLNTVYKMTLNSTRKGGAGAGSLVWQVFPQGAEFMDDGYAVYLTRAHT 405

Query: 399 IYQLIIEQSCRLA 412
             ++I  QS RLA
Sbjct: 406 ASKIISLQSKRLA 417

BLAST of Lsi10G008980 vs. ExPASy TrEMBL
Match: A0A5D3C3G0 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G001850 PE=3 SV=1)

HSP 1 Score: 866.3 bits (2237), Expect = 5.4e-248
Identity = 410/431 (95.13%), Postives = 420/431 (97.45%), Query Frame = 0

Query: 1   MAGNGLFYPILGFASCLAFIYMSFGDFRIGGETKIPIVDFVERNGTQFMLNGKAFYINGW 60
           MAGNGLFYPILGFASCLAFIY+SFGD RIG    IPIVDFVERNGTQFMLNGKAFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDLRIGDTKVIPIVDFVERNGTQFMLNGKAFYINGW 60

Query: 61  NSYWFMDHAVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120
           NSYWFMDH+VEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA
Sbjct: 61  NSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180
           LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 181 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240
           KNYIKTVLTRKNSITGIEYR+DPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID
Sbjct: 181 KNYIKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 241 KKHLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHD 300
           KKHLLTVGLEGFYGPNS KKSTVNPEEWASRLGSDFIRNSEIP+VDFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFASVHIYPDHWFHD 300

Query: 301 QDFEDELRFVSKWMLSHIEDGDIEMKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIY 360
           QDFEDEL+FVSKWMLSHIEDGD E+KKPVMFTEFGLSDLNKGFT AQRD FYKTVYDVIY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQRDSFYKTVYDVIY 360

Query: 361 RSARKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYQLIIEQSCRLARIGGDIQQL 420
           +SA++KRSGAGSLAWQFLVEGMEESNDDFGIVPWER SIYQLIIEQSCRLARIGGD QQL
Sbjct: 361 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLARIGGDTQQL 420

Query: 421 KALKYVCSQRW 432
           KALKYVC+QRW
Sbjct: 421 KALKYVCAQRW 431

BLAST of Lsi10G008980 vs. ExPASy TrEMBL
Match: A0A1S3BJC2 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103490531 PE=3 SV=1)

HSP 1 Score: 866.3 bits (2237), Expect = 5.4e-248
Identity = 410/431 (95.13%), Postives = 420/431 (97.45%), Query Frame = 0

Query: 1   MAGNGLFYPILGFASCLAFIYMSFGDFRIGGETKIPIVDFVERNGTQFMLNGKAFYINGW 60
           MAGNGLFYPILGFASCLAFIY+SFGD RIG    IPIVDFVERNGTQFMLNGKAFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDLRIGDTKVIPIVDFVERNGTQFMLNGKAFYINGW 60

Query: 61  NSYWFMDHAVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120
           NSYWFMDH+VEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA
Sbjct: 61  NSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180
           LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 181 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240
           KNYIKTVLTRKNSITGIEYR+DPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID
Sbjct: 181 KNYIKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 241 KKHLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHD 300
           KKHLLTVGLEGFYGPNS KKSTVNPEEWASRLGSDFIRNSEIP+VDFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFASVHIYPDHWFHD 300

Query: 301 QDFEDELRFVSKWMLSHIEDGDIEMKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIY 360
           QDFEDEL+FVSKWMLSHIEDGD E+KKPVMFTEFGLSDLNKGFT AQRD FYKTVYDVIY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQRDSFYKTVYDVIY 360

Query: 361 RSARKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYQLIIEQSCRLARIGGDIQQL 420
           +SA++KRSGAGSLAWQFLVEGMEESNDDFGIVPWER SIYQLIIEQSCRLARIGGD QQL
Sbjct: 361 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLARIGGDTQQL 420

Query: 421 KALKYVCSQRW 432
           KALKYVC+QRW
Sbjct: 421 KALKYVCAQRW 431

BLAST of Lsi10G008980 vs. ExPASy TrEMBL
Match: A0A0A0KB35 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G127990 PE=3 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 2.7e-247
Identity = 408/431 (94.66%), Postives = 419/431 (97.22%), Query Frame = 0

Query: 1   MAGNGLFYPILGFASCLAFIYMSFGDFRIGGETKIPIVDFVERNGTQFMLNGKAFYINGW 60
           MAGNGLFYPILGFASCLAFIY+SFGD RIG    IPIVDFVERNGTQFMLNGKAFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDLRIGDTKVIPIVDFVERNGTQFMLNGKAFYINGW 60

Query: 61  NSYWFMDHAVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120
           NSYWFMDH+VEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA
Sbjct: 61  NSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180
           LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 181 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240
           KNY+KTVLTRKNSITGIEYR+DPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID
Sbjct: 181 KNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 241 KKHLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHD 300
           KKHLLTVGLEGFYGPNS KKSTVNPEEWASRLGSDFIRNSEI +VDFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVHIYPDHWFHD 300

Query: 301 QDFEDELRFVSKWMLSHIEDGDIEMKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIY 360
           QDFEDEL+FVSKWMLSHIEDGD E+KKPVMFTEFGLSDLNKGFTPAQRD FYKTVYDVIY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSFYKTVYDVIY 360

Query: 361 RSARKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYQLIIEQSCRLARIGGDIQQL 420
           +SA++ RSGAGSLAWQFLVEGMEESNDDFGIVPWER SIYQLIIEQSCRLARIGGD QQL
Sbjct: 361 KSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLARIGGDTQQL 420

Query: 421 KALKYVCSQRW 432
           KALKYVC+QRW
Sbjct: 421 KALKYVCAQRW 431

BLAST of Lsi10G008980 vs. ExPASy TrEMBL
Match: A0A5A7V836 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold89G003960 PE=3 SV=1)

HSP 1 Score: 863.6 bits (2230), Expect = 3.5e-247
Identity = 408/431 (94.66%), Postives = 420/431 (97.45%), Query Frame = 0

Query: 1   MAGNGLFYPILGFASCLAFIYMSFGDFRIGGETKIPIVDFVERNGTQFMLNGKAFYINGW 60
           MAGNGLFYPILGFASCLAFIY+SFGD RIG    IPIVDFVERNGTQF+LNGKAFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDLRIGDTKVIPIVDFVERNGTQFILNGKAFYINGW 60

Query: 61  NSYWFMDHAVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120
           NSYWFMDH+VEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA
Sbjct: 61  NSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180
           LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 181 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240
           KNYIKTVLTRKNSITGIEYR+DPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID
Sbjct: 181 KNYIKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 241 KKHLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHD 300
           KKHLLTVGLEGFYGPNS KKSTVNPEEWASRLGSDFIRNSEIP+VDFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFASVHIYPDHWFHD 300

Query: 301 QDFEDELRFVSKWMLSHIEDGDIEMKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIY 360
           QDFEDEL+FVSKWMLSHIEDGD E+KKPVMFTEFGLSDLNKGFT AQRD FYKTVYDVIY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQRDSFYKTVYDVIY 360

Query: 361 RSARKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYQLIIEQSCRLARIGGDIQQL 420
           +SA++KRSGAGSLAWQFLVEGMEESNDDFGIVPWER SIYQLIIEQSCRLA+IGGD QQL
Sbjct: 361 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLAKIGGDTQQL 420

Query: 421 KALKYVCSQRW 432
           KALKYVC+QRW
Sbjct: 421 KALKYVCAQRW 431

BLAST of Lsi10G008980 vs. ExPASy TrEMBL
Match: A0A6J1CF50 (Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111011126 PE=3 SV=1)

HSP 1 Score: 844.3 bits (2180), Expect = 2.2e-241
Identity = 397/427 (92.97%), Postives = 415/427 (97.19%), Query Frame = 0

Query: 1   MAGNGLFYPILGFASCLAFIYMSFGDFRIGGETKIPIVDFVERNGTQFMLNGKAFYINGW 60
           MAGNGLFYPILGFASCLAFIY+SFG+ ++ G ++ PI+DFVERNGTQFMLNGKAFYINGW
Sbjct: 2   MAGNGLFYPILGFASCLAFIYLSFGELKL-GYSEAPIIDFVERNGTQFMLNGKAFYINGW 61

Query: 61  NSYWFMDHAVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120
           NSYWFMDHAVEEYRKPR+RAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA
Sbjct: 62  NSYWFMDHAVEEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 121

Query: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180
           LDHVIAE+RQHGIRLLL LVNNLQAYGGKTQYVKWAWQ+GVGLSSSNDSFFYDPSIRIYF
Sbjct: 122 LDHVIAEARQHGIRLLLCLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYF 181

Query: 181 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240
           KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEM+AYIKSID
Sbjct: 182 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID 241

Query: 241 KKHLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHD 300
           KKHLLTVGLEGFYGP S KKSTVNPEEWASRLGSDF+RNS IP+VDFASVHIYPDHWFHD
Sbjct: 242 KKHLLTVGLEGFYGPTSPKKSTVNPEEWASRLGSDFVRNSAIPHVDFASVHIYPDHWFHD 301

Query: 301 QDFEDELRFVSKWMLSHIEDGDIEMKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIY 360
           QDFEDELRFVSKWMLSHI DGDIEMKKPVMFTEFGLS+LN GFTP QRDRFYKTVYDVIY
Sbjct: 302 QDFEDELRFVSKWMLSHIADGDIEMKKPVMFTEFGLSNLNIGFTPEQRDRFYKTVYDVIY 361

Query: 361 RSARKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYQLIIEQSCRLARIGGDIQQL 420
           +SA+KKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIY+LIIEQSCRLAR+GGD QQL
Sbjct: 362 KSAKKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLARVGGDTQQL 421

Query: 421 KALKYVC 428
           KALKYVC
Sbjct: 422 KALKYVC 427

BLAST of Lsi10G008980 vs. NCBI nr
Match: XP_038902035.1 (mannan endo-1,4-beta-mannosidase 2-like [Benincasa hispida])

HSP 1 Score: 880.6 bits (2274), Expect = 5.7e-252
Identity = 417/431 (96.75%), Postives = 424/431 (98.38%), Query Frame = 0

Query: 1   MAGNGLFYPILGFASCLAFIYMSFGDFRIGGETKIPIVDFVERNGTQFMLNGKAFYINGW 60
           MAGNGLFYPILGFASCLAFIY+SFGDFRIG    IPIV+FVERNGTQFMLNGKAFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDFRIGDTKVIPIVNFVERNGTQFMLNGKAFYINGW 60

Query: 61  NSYWFMDHAVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120
           NSYWFMDHAVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA
Sbjct: 61  NSYWFMDHAVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180
           LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 181 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240
           KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID
Sbjct: 181 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 241 KKHLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHD 300
           KKHLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDFIRNSEIP VDFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDFIRNSEIPNVDFASVHIYPDHWFHD 300

Query: 301 QDFEDELRFVSKWMLSHIEDGDIEMKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIY 360
           QDFEDEL+FVSKWMLSHIEDGDIEMKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDIEMKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIY 360

Query: 361 RSARKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYQLIIEQSCRLARIGGDIQQL 420
           +SA++KRSGAGSLAWQFLVEGMEESNDDFGIVPWER SIYQLIIEQSCRLARIGGD QQL
Sbjct: 361 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERQSIYQLIIEQSCRLARIGGDAQQL 420

Query: 421 KALKYVCSQRW 432
           KALKYVC+QRW
Sbjct: 421 KALKYVCAQRW 431

BLAST of Lsi10G008980 vs. NCBI nr
Match: XP_008448293.1 (PREDICTED: mannan endo-1,4-beta-mannosidase 2 [Cucumis melo] >TYK04966.1 mannan endo-1,4-beta-mannosidase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 866.3 bits (2237), Expect = 1.1e-247
Identity = 410/431 (95.13%), Postives = 420/431 (97.45%), Query Frame = 0

Query: 1   MAGNGLFYPILGFASCLAFIYMSFGDFRIGGETKIPIVDFVERNGTQFMLNGKAFYINGW 60
           MAGNGLFYPILGFASCLAFIY+SFGD RIG    IPIVDFVERNGTQFMLNGKAFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDLRIGDTKVIPIVDFVERNGTQFMLNGKAFYINGW 60

Query: 61  NSYWFMDHAVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120
           NSYWFMDH+VEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA
Sbjct: 61  NSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180
           LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 181 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240
           KNYIKTVLTRKNSITGIEYR+DPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID
Sbjct: 181 KNYIKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 241 KKHLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHD 300
           KKHLLTVGLEGFYGPNS KKSTVNPEEWASRLGSDFIRNSEIP+VDFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFASVHIYPDHWFHD 300

Query: 301 QDFEDELRFVSKWMLSHIEDGDIEMKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIY 360
           QDFEDEL+FVSKWMLSHIEDGD E+KKPVMFTEFGLSDLNKGFT AQRD FYKTVYDVIY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQRDSFYKTVYDVIY 360

Query: 361 RSARKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYQLIIEQSCRLARIGGDIQQL 420
           +SA++KRSGAGSLAWQFLVEGMEESNDDFGIVPWER SIYQLIIEQSCRLARIGGD QQL
Sbjct: 361 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLARIGGDTQQL 420

Query: 421 KALKYVCSQRW 432
           KALKYVC+QRW
Sbjct: 421 KALKYVCAQRW 431

BLAST of Lsi10G008980 vs. NCBI nr
Match: XP_004139946.1 (mannan endo-1,4-beta-mannosidase 2 [Cucumis sativus] >KGN46738.1 hypothetical protein Csa_021081 [Cucumis sativus])

HSP 1 Score: 864.0 bits (2231), Expect = 5.5e-247
Identity = 408/431 (94.66%), Postives = 419/431 (97.22%), Query Frame = 0

Query: 1   MAGNGLFYPILGFASCLAFIYMSFGDFRIGGETKIPIVDFVERNGTQFMLNGKAFYINGW 60
           MAGNGLFYPILGFASCLAFIY+SFGD RIG    IPIVDFVERNGTQFMLNGKAFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDLRIGDTKVIPIVDFVERNGTQFMLNGKAFYINGW 60

Query: 61  NSYWFMDHAVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120
           NSYWFMDH+VEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA
Sbjct: 61  NSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180
           LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 181 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240
           KNY+KTVLTRKNSITGIEYR+DPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID
Sbjct: 181 KNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 241 KKHLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHD 300
           KKHLLTVGLEGFYGPNS KKSTVNPEEWASRLGSDFIRNSEI +VDFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVHIYPDHWFHD 300

Query: 301 QDFEDELRFVSKWMLSHIEDGDIEMKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIY 360
           QDFEDEL+FVSKWMLSHIEDGD E+KKPVMFTEFGLSDLNKGFTPAQRD FYKTVYDVIY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSFYKTVYDVIY 360

Query: 361 RSARKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYQLIIEQSCRLARIGGDIQQL 420
           +SA++ RSGAGSLAWQFLVEGMEESNDDFGIVPWER SIYQLIIEQSCRLARIGGD QQL
Sbjct: 361 KSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLARIGGDTQQL 420

Query: 421 KALKYVCSQRW 432
           KALKYVC+QRW
Sbjct: 421 KALKYVCAQRW 431

BLAST of Lsi10G008980 vs. NCBI nr
Match: KAA0062055.1 (mannan endo-1,4-beta-mannosidase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 863.6 bits (2230), Expect = 7.2e-247
Identity = 408/431 (94.66%), Postives = 420/431 (97.45%), Query Frame = 0

Query: 1   MAGNGLFYPILGFASCLAFIYMSFGDFRIGGETKIPIVDFVERNGTQFMLNGKAFYINGW 60
           MAGNGLFYPILGFASCLAFIY+SFGD RIG    IPIVDFVERNGTQF+LNGKAFYINGW
Sbjct: 1   MAGNGLFYPILGFASCLAFIYLSFGDLRIGDTKVIPIVDFVERNGTQFILNGKAFYINGW 60

Query: 61  NSYWFMDHAVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120
           NSYWFMDH+VEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA
Sbjct: 61  NSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120

Query: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180
           LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF
Sbjct: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180

Query: 181 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240
           KNYIKTVLTRKNSITGIEYR+DPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID
Sbjct: 181 KNYIKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240

Query: 241 KKHLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHD 300
           KKHLLTVGLEGFYGPNS KKSTVNPEEWASRLGSDFIRNSEIP+VDFASVHIYPDHWFHD
Sbjct: 241 KKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFASVHIYPDHWFHD 300

Query: 301 QDFEDELRFVSKWMLSHIEDGDIEMKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIY 360
           QDFEDEL+FVSKWMLSHIEDGD E+KKPVMFTEFGLSDLNKGFT AQRD FYKTVYDVIY
Sbjct: 301 QDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQRDSFYKTVYDVIY 360

Query: 361 RSARKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYQLIIEQSCRLARIGGDIQQL 420
           +SA++KRSGAGSLAWQFLVEGMEESNDDFGIVPWER SIYQLIIEQSCRLA+IGGD QQL
Sbjct: 361 KSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLAKIGGDTQQL 420

Query: 421 KALKYVCSQRW 432
           KALKYVC+QRW
Sbjct: 421 KALKYVCAQRW 431

BLAST of Lsi10G008980 vs. NCBI nr
Match: XP_022140455.1 (mannan endo-1,4-beta-mannosidase 2-like [Momordica charantia])

HSP 1 Score: 844.3 bits (2180), Expect = 4.5e-241
Identity = 397/427 (92.97%), Postives = 415/427 (97.19%), Query Frame = 0

Query: 1   MAGNGLFYPILGFASCLAFIYMSFGDFRIGGETKIPIVDFVERNGTQFMLNGKAFYINGW 60
           MAGNGLFYPILGFASCLAFIY+SFG+ ++ G ++ PI+DFVERNGTQFMLNGKAFYINGW
Sbjct: 2   MAGNGLFYPILGFASCLAFIYLSFGELKL-GYSEAPIIDFVERNGTQFMLNGKAFYINGW 61

Query: 61  NSYWFMDHAVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 120
           NSYWFMDHAVEEYRKPR+RAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA
Sbjct: 62  NSYWFMDHAVEEYRKPRVRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKA 121

Query: 121 LDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYF 180
           LDHVIAE+RQHGIRLLL LVNNLQAYGGKTQYVKWAWQ+GVGLSSSNDSFFYDPSIRIYF
Sbjct: 122 LDHVIAEARQHGIRLLLCLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFYDPSIRIYF 181

Query: 181 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSID 240
           KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEM+AYIKSID
Sbjct: 182 KNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMSAYIKSID 241

Query: 241 KKHLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHD 300
           KKHLLTVGLEGFYGP S KKSTVNPEEWASRLGSDF+RNS IP+VDFASVHIYPDHWFHD
Sbjct: 242 KKHLLTVGLEGFYGPTSPKKSTVNPEEWASRLGSDFVRNSAIPHVDFASVHIYPDHWFHD 301

Query: 301 QDFEDELRFVSKWMLSHIEDGDIEMKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIY 360
           QDFEDELRFVSKWMLSHI DGDIEMKKPVMFTEFGLS+LN GFTP QRDRFYKTVYDVIY
Sbjct: 302 QDFEDELRFVSKWMLSHIADGDIEMKKPVMFTEFGLSNLNIGFTPEQRDRFYKTVYDVIY 361

Query: 361 RSARKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYQLIIEQSCRLARIGGDIQQL 420
           +SA+KKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIY+LIIEQSCRLAR+GGD QQL
Sbjct: 362 KSAKKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYRLIIEQSCRLARVGGDTQQL 421

Query: 421 KALKYVC 428
           KALKYVC
Sbjct: 422 KALKYVC 427

BLAST of Lsi10G008980 vs. TAIR 10
Match: AT2G20680.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 693.0 bits (1787), Expect = 1.6e-199
Identity = 318/428 (74.30%), Postives = 373/428 (87.15%), Query Frame = 0

Query: 3   GNGLFYPILGFASCLAFIYMSFGDFRIGGETKIPIVDFVERNGTQFMLNGKAFYINGWNS 62
           GNG   PILGF +C+AFIY+SFGD   G +T+  +  FV+RNGTQF+++GKA Y+NGWNS
Sbjct: 6   GNGPVIPILGFLTCVAFIYLSFGDLWFGLKTEGELA-FVKRNGTQFVVDGKALYVNGWNS 65

Query: 63  YWFMDHAVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKALD 122
           YWFMDHAV ++ + R+ AML+A  KMGLTVCRTWAFNDGGYNALQ+SPGRF+ERVFKALD
Sbjct: 66  YWFMDHAVNDHSRHRVSAMLEAGAKMGLTVCRTWAFNDGGYNALQISPGRFDERVFKALD 125

Query: 123 HVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKN 182
           HVIAE++ HG+RLLLSLVNNLQAYGGKTQYV WAWQ+GVGLSSSNDSFF+DPSIR YFKN
Sbjct: 126 HVIAEAKTHGVRLLLSLVNNLQAYGGKTQYVNWAWQEGVGLSSSNDSFFFDPSIRRYFKN 185

Query: 183 YIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSIDKK 242
           Y+  +LTRKNS+TGIEYR+DPTIF WELINEPRCM+D SGDTLQ+WI EMTA+IKSID K
Sbjct: 186 YLTVLLTRKNSLTGIEYRNDPTIFAWELINEPRCMSDVSGDTLQDWINEMTAFIKSIDNK 245

Query: 243 HLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHDQD 302
           HLLTVGLEGFYGP+S KK TVNPE WAS LGSDF+RNS+ P +DFASVHIYPDHWFHDQ 
Sbjct: 246 HLLTVGLEGFYGPSSPKKLTVNPERWASELGSDFVRNSDSPNIDFASVHIYPDHWFHDQG 305

Query: 303 FEDELRFVSKWMLSHIEDGDIEMKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYRS 362
           FE++L+FV KWMLSHIEDGD E+KKPV+FTEFGLS+LNK + P+QRDRFY+T++DVIY+S
Sbjct: 306 FEEKLKFVVKWMLSHIEDGDKELKKPVLFTEFGLSNLNKDYDPSQRDRFYRTIFDVIYKS 365

Query: 363 ARKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPSIYQLIIEQSCRLARIGGDIQQLKA 422
           A++KRSGAG+L WQFL+EGME  NDDFGIVPWE+ SI +L+IEQSCRL+RI G     K 
Sbjct: 366 AKRKRSGAGTLVWQFLIEGMEGFNDDFGIVPWEQDSIQRLMIEQSCRLSRITGRHLLDKK 425

Query: 423 LKYVCSQR 431
              +CS R
Sbjct: 426 SIEMCSHR 432

BLAST of Lsi10G008980 vs. TAIR 10
Match: AT4G28320.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 645.6 bits (1664), Expect = 2.9e-185
Identity = 295/421 (70.07%), Postives = 359/421 (85.27%), Query Frame = 0

Query: 10  ILGFASCLAFIYMSFGDFRIGGETKIPIVDFVERNGTQFMLNGKAFYINGWNSYWFMDHA 69
           ILGF  C AFIY+SF D  +  + K  +  FV+RNGTQF+++ K  Y+NGWNSYWFMDHA
Sbjct: 12  ILGFFICAAFIYLSFRDLWLNHKGKAKL-GFVKRNGTQFVVDDKPLYVNGWNSYWFMDHA 71

Query: 70  VEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKALDHVIAESR 129
           V+E+ +  +  ML+A  KMGLTVCRTWAFNDGGYNALQ+SPGRF+ERVF+ALDHVIAE+R
Sbjct: 72  VDEHSRNLVGEMLEAGAKMGLTVCRTWAFNDGGYNALQISPGRFDERVFQALDHVIAEAR 131

Query: 130 QHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKNYIKTVLT 189
           +H +RLLLSLVNNLQAYGGKTQYVKWAWQ+GVGLSSSNDSFF+DPSIR YFKNY+K +LT
Sbjct: 132 KHDVRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSNDSFFFDPSIRNYFKNYLKVLLT 191

Query: 190 RKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGL 249
           RKNS+TGIEYR+DPTIF WELINEPRC TD SG TLQ+WI+EMT +IKSID KHLLTVGL
Sbjct: 192 RKNSVTGIEYRNDPTIFAWELINEPRCTTDVSGKTLQDWIDEMTGFIKSIDDKHLLTVGL 251

Query: 250 EGFYGPNSRKKSTVNPEEWASRLGSDFIRNSEIPYVDFASVHIYPDHWFHDQDFEDELRF 309
           EGFYGPNS K  TVNPE+WAS+LG+DF++NS    +DFASVHIYPDHWFH+Q FE++L+F
Sbjct: 252 EGFYGPNSPKGLTVNPEQWASQLGTDFVQNSNSSNIDFASVHIYPDHWFHNQTFEEKLKF 311

Query: 310 VSKWMLSHIEDGDIEMKKPVMFTEFGLSDLNKGFTPAQRDRFYKTVYDVIYRSARKKRSG 369
           V KWM SHIEDG  E+KKPV+FTEFGLS+ NK + P+QRD+FY+ ++DV+Y+SA++K+SG
Sbjct: 312 VVKWMQSHIEDGLKELKKPVLFTEFGLSNQNKDYEPSQRDKFYRIIFDVVYKSAKRKKSG 371

Query: 370 AGSLAWQFLVEGMEESNDDFGIVPWERPSIYQLIIEQSCRLARIGGDIQQLKALKYVCSQ 429
           AG+L WQ  +EGME  NDDFGIVP E+ SIY+L+IEQSCRL ++ G +++ K LK +CS 
Sbjct: 372 AGTLVWQLFMEGMETFNDDFGIVPHEQDSIYKLMIEQSCRLGKVTGRLKEQK-LKELCSH 430

Query: 430 R 431
           R
Sbjct: 432 R 430

BLAST of Lsi10G008980 vs. TAIR 10
Match: AT5G01930.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 389.8 bits (1000), Expect = 2.8e-108
Identity = 178/373 (47.72%), Postives = 256/373 (68.63%), Query Frame = 0

Query: 39  DFVERNGTQFMLNGKAFYINGWNSYWFMDHAVEEYRKPRIRAMLQAARKMGLTVCRTWAF 98
           + V+R G QF LNG+ FY+NG+N+YW M  A +   + ++  + Q A  +G+TV RTWAF
Sbjct: 46  EMVQRKGMQFTLNGQPFYVNGFNTYWMMTLAADNSTRGKVTEVFQQASAVGMTVGRTWAF 105

Query: 99  NDGGYNALQVSPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQ 158
           NDG + ALQ SP  ++E VFKALD V++E+R++ IRL+LSLVNN  AYGGK QYVKW   
Sbjct: 106 NDGQWRALQKSPSVYDEEVFKALDFVLSEARKYKIRLILSLVNNWDAYGGKAQYVKWGNA 165

Query: 159 DGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMT 218
            G+ L +S+D FF +P++R +++++++TVL R N+ T I Y++DPTIF WEL+NEPRC +
Sbjct: 166 SGLNL-TSDDDFFTNPTLRNFYQSHVRTVLNRVNTFTNITYKNDPTIFAWELMNEPRCPS 225

Query: 219 DASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDFIR 278
           D SGD LQ WI+EM  ++KS+D KHL+ +GLEGFYGP++  ++  NP  +A+++G+DFIR
Sbjct: 226 DPSGDKLQSWIQEMAVFVKSLDAKHLVEIGLEGFYGPSAPARTRFNPNPYAAQVGTDFIR 285

Query: 279 NSEIPYVDFASVHIYPDHWFHDQDFEDELRFVSKWMLSHIEDGDIEMKKPVMFTEFGLSD 338
           N+++  +DFASVH+YPD W         L F S WM +H+ED ++ +  PV+FTEFG+S 
Sbjct: 286 NNQVLGIDFASVHVYPDSWISPAVSNSFLEFTSSWMQAHVEDAEMYLGMPVLFTEFGVSA 345

Query: 339 LNKGFTPAQRDRFYKTVYDVIYRSARKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPS 398
            + GF  + RD    TVY +   S RK  +GAGSL WQ   +G E  +D + +      +
Sbjct: 346 HDPGFNTSFRDMMLNTVYKMTLNSTRKGGAGAGSLVWQVFPQGAEFMDDGYAVYLTRAHT 405

Query: 399 IYQLIIEQSCRLA 412
             ++I  QS RLA
Sbjct: 406 ASKIISLQSKRLA 417

BLAST of Lsi10G008980 vs. TAIR 10
Match: AT5G66460.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 384.0 bits (985), Expect = 1.6e-106
Identity = 178/383 (46.48%), Postives = 250/383 (65.27%), Query Frame = 0

Query: 31  GETKIPIVDFVERNGTQFMLNGKAFYINGWNSYWFMDHAVEEYRKPRIRAMLQAARKMGL 90
           G   +P   FV   G QF LNG  +Y NG+N+YW M  A +  ++ +I    Q A + GL
Sbjct: 22  GTDALPSGGFVRTKGVQFSLNGYPYYANGFNAYWLMYVASDPSQRSKISTAFQDASRHGL 81

Query: 91  TVCRTWAFNDGGYNALQVSPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKT 150
           TV RTWAF+DGGY ALQ SPG +NE +F+ LD  +AE+R+HGI+++LS  NN +++GG+ 
Sbjct: 82  TVARTWAFSDGGYRALQYSPGSYNEDMFQGLDFALAEARRHGIKIILSFANNYESFGGRK 141

Query: 151 QYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKNYIKTVLTRKNSITGIEYRDDPTIFGWEL 210
           QYV WA   G  +SS +D FF D  ++ ++KN+IK VL R N+ T + Y+DDPTI  WEL
Sbjct: 142 QYVDWARSRGRPVSSEDD-FFTDSLVKDFYKNHIKAVLNRFNTFTKVHYKDDPTIMAWEL 201

Query: 211 INEPRCMTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSRKKSTVNPEEWAS 270
           +NEPRC +D SG  +Q WI EM A++KS+D+ HLL  GLEGFYG +S +  T+NP     
Sbjct: 202 MNEPRCPSDPSGRAIQAWITEMAAHVKSLDRNHLLEAGLEGFYGQSSPQSKTLNP---PG 261

Query: 271 RLGSDFIRNSEIPYVDFASVHIYPDHWFHDQDFEDELRFVSKWMLSHIEDGDIEMKKPVM 330
           + G+DFI N+ IP +DF +VH YPD WF D   + ++ F++KW+ +HI+D    + KP++
Sbjct: 262 QFGTDFIANNRIPGIDFVTVHSYPDEWFPDSSEQSQMDFLNKWLDAHIQDAQNVLHKPII 321

Query: 331 FTEFGLSDLNKGFTPAQRDRFYKTVYDVIYRSARKKRSGAGSLAWQFLVEGMEESNDDFG 390
             EFG S    G+TPAQRD  + TVY  IY SA++  + AG L WQ LV G++   D +G
Sbjct: 322 LAEFGKSMKKPGYTPAQRDIVFNTVYSKIYGSAKRGGAAAGGLFWQLLVNGIDNFQDGYG 381

Query: 391 IVPWERPSIYQLIIEQSCRLARI 414
           I+  +  S   +I +QS +L  I
Sbjct: 382 IILSQSSSTVNVISQQSRKLTLI 400

BLAST of Lsi10G008980 vs. TAIR 10
Match: AT3G10890.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 362.1 bits (928), Expect = 6.3e-100
Identity = 168/377 (44.56%), Postives = 243/377 (64.46%), Query Frame = 0

Query: 40  FVERNGTQFMLNGKAFYINGWNSYWFMDHAVEEYRKPRIRAMLQAARKMGLTVCRTWAFN 99
           FV R G QF+LNGK FY NG+N+YW    A +   + +I  + Q A    LT+ RTW F 
Sbjct: 31  FVSRKGVQFILNGKPFYANGFNAYWLAYEATDSTTRFKITYVFQNATIHDLTIVRTWGFR 90

Query: 100 DGGYNALQVSPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQD 159
           DGGY ALQ++PG ++E+ F+ LD  IAE+++ GI+++++ VNN   +GG+ QYV WA   
Sbjct: 91  DGGYRALQIAPGVYDEKTFQGLDFAIAEAKRLGIKMIITFVNNYSDFGGRKQYVDWAKNT 150

Query: 160 GVGLSSSNDSFFYDPSIRIYFKNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTD 219
           G  + SS+D F+ +P ++ Y+KN++KT++ R N+ T +EY+D+PTI GWEL+NEP+C  D
Sbjct: 151 GQNV-SSDDDFYTNPLVKQYYKNHVKTMVNRVNTFTKVEYKDEPTIMGWELMNEPQCRAD 210

Query: 220 ASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNS-RKKSTVNPEEWASRLGSDFIR 279
            SG TL  W+ EM  Y+KS+D KHLL+ GLEGFYG +S ++K+++NP   A+ LG+DFI 
Sbjct: 211 PSGKTLTAWMNEMALYVKSVDSKHLLSTGLEGFYGDSSPQRKTSLNPVA-ANVLGTDFIA 270

Query: 280 NSEIPYVDFASVHIYPDHWFHDQDFEDELRFVSKWMLSHIEDGDIEMKKPVMFTEFGLSD 339
           N ++  +DFAS+H YPD WF + D +  L  + KW+  H+ED    +KKP++  EFG   
Sbjct: 271 NHKLDAIDFASIHSYPDLWFPNLDEKSRLNLLRKWLECHLEDAQNILKKPLILGEFGKPT 330

Query: 340 LNKGFTPAQRDRFYKTVYDVIYRSARKKRSGAGSLAWQFLVEGMEESNDDFGIVPWERPS 399
              G+T AQRD  +   +D IY SA K    AG+L W  + +GM    D   IV  E  +
Sbjct: 331 NTPGYTQAQRDAVFNATFDTIYESAEKGGPAAGALFWHVISDGMNNFKDPLSIVLSENST 390

Query: 400 IYQLIIEQSCRLARIGG 416
              +I E+S +L  I G
Sbjct: 391 TVNIITEESRKLGLIRG 405

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7Y2232.2e-19874.30Mannan endo-1,4-beta-mannosidase 2 OS=Arabidopsis thaliana OX=3702 GN=MAN2 PE=2 ... [more]
Q9M0H64.0e-18470.07Mannan endo-1,4-beta-mannosidase 5 OS=Arabidopsis thaliana OX=3702 GN=MAN5 PE=2 ... [more]
Q0JJD46.7e-15558.37Mannan endo-1,4-beta-mannosidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=M... [more]
Q0DCM54.2e-11748.83Mannan endo-1,4-beta-mannosidase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=M... [more]
Q9LZV34.0e-10747.72Mannan endo-1,4-beta-mannosidase 6 OS=Arabidopsis thaliana OX=3702 GN=MAN6 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A5D3C3G05.4e-24895.13Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3BJC25.4e-24895.13Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103490531 PE=3 SV... [more]
A0A0A0KB352.7e-24794.66Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G127990 PE=3... [more]
A0A5A7V8363.5e-24794.66Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A6J1CF502.2e-24192.97Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111011126 ... [more]
Match NameE-valueIdentityDescription
XP_038902035.15.7e-25296.75mannan endo-1,4-beta-mannosidase 2-like [Benincasa hispida][more]
XP_008448293.11.1e-24795.13PREDICTED: mannan endo-1,4-beta-mannosidase 2 [Cucumis melo] >TYK04966.1 mannan ... [more]
XP_004139946.15.5e-24794.66mannan endo-1,4-beta-mannosidase 2 [Cucumis sativus] >KGN46738.1 hypothetical pr... [more]
KAA0062055.17.2e-24794.66mannan endo-1,4-beta-mannosidase 2 [Cucumis melo var. makuwa][more]
XP_022140455.14.5e-24192.97mannan endo-1,4-beta-mannosidase 2-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT2G20680.11.6e-19974.30Glycosyl hydrolase superfamily protein [more]
AT4G28320.12.9e-18570.07Glycosyl hydrolase superfamily protein [more]
AT5G01930.12.8e-10847.72Glycosyl hydrolase superfamily protein [more]
AT5G66460.11.6e-10646.48Glycosyl hydrolase superfamily protein [more]
AT3G10890.16.3e-10044.56Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 52..375
e-value: 2.0E-14
score: 53.7
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 38..414
e-value: 4.8E-134
score: 449.0
NoneNo IPR availablePANTHERPTHR31451:SF56GLYCOSYL HYDROLASE SUPERFAMILY PROTEIN-RELATEDcoord: 1..429
IPR045053Mannan endo-1,4-beta-mannosidase-likePANTHERPTHR31451FAMILY NOT NAMEDcoord: 1..429
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 39..396

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G008980.1Lsi10G008980.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds