Lsi10G008740 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G008740
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAuxin-responsive GH3 family protein
Locationchr10: 12951851 .. 12955495 (-)
RNA-Seq ExpressionLsi10G008740
SyntenyLsi10G008740
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGTATTCTGTCGTACATTGAATTCGGCGAATGCAACCTTTTGCAGTAGTACATAGTTTAATTTTGTGAGTTTTTTAAACCCATAAAGACGTAACCTAAGCCTAAGCCCCTTTACTAGAAAAACAAACCATATTATTTGTGGGTTGTGGAGAGAGAGAGAGAGAGAGAAAAAAAAAAAAGAAAGTAAAAGAAGGGATATATGTAAAAATGGAAGGTGAGGAAGGAGGAGGTAAGGAATTGATGAAGATTGAGGAATTATGTGAAGAGGAAGAGGTGATTGATTGGTTCGAGCAAGTTTCGTCAGAGGCAGGCGTGGCTCAATCCCGGACTCTCCGTAAGATTCTGCAACAGAATTGTGGTGTTGAGTACCTGAAACAATGGCTTGGAGATGTCAATATCCATGAAATTCCTGATGACTTTACTTTACAATCTCTCTTCACTTCCTTAATCCCCCTCTCTTCCCATTCTGATTTCGAACCCTTCCTCCAACGAATCGCACATGGCGACTCTTCTCCTTTGCTCACTCAACAACCCATCAACACTCTCTCTTTAAGGTATTTTCCTTCTTTTTTTTTTTTTTTTTTTTTTAATTTTTATTAAATTAAGTAAAGATAGTTGCAGTCCAATCTAACACCTAATTTCGTCTTTCGTAGTTCTGGCACTACCGAAGGAAGACAAAAGTACGTTCCCTTTACTCGCCATAGCGCTCAGACTACTCTTCTCATCTTCCGCTTAGCTGCAGCTTATAGATCCAGGTACCAACGCAAGACAACCCATTTATTATATATATTTTACTGGGGAATAATTAAAAAAATATATATTTATATATATTGTGAAAAAAATGACTAGGGTTTATCCCACAAGGGAAGGAGGGAAGATTCTAGAGTTCATTTACAGTAGCAAACAAACGAAAACAAAAGGAGGAATAACAACGGGAACGGCAACAACACATTATTACGCAAGTGAGGAATTCAAAATCAAACAAGTGAAAACCAAATCCTTCACGTGCAGTCCACAAGAAGTGATATCAGGATGTGACTATAAACAATCAACTTACTGCCATCTCCTACTAGGCCTATTCTATTCAGAAGAAGTAGAGTTCGTGACCTCAACATTTGCGTACACAATAGTCCAAGCTTTAAACCAATTAGAAGAGAGTTGGGAAGAAATGTGTGAAGACATTACAAATGCTACACTTTCCTCAAGAATTGATATACCCAAAATGAGAAAGGGTGTTTTAAAAGTAATGAGTGGGAATCCGGGTTTGGGTTGGAAAATAGGAAGGGTTTGTGAAGAGCTAAAAAGAGAGGATTGGTTGGGTTTAATACCAAAGCTATGGCCAAATTGCAAATATGTATATTCGATAATGACGGGATCAATGCAGCCGTATTTGAAGAAATTGAGACATTATGCTGGGGGATTGCCTTTGGTTAGTGGTGATTATGGATCAACGGAGAGTTGGATTGGGGTTAATGTCGACCCTTATTTGCCACCTGAAAATGTTACTTTTGCGGTCATTCCTACTTTCTCTTATTTTGAATTCATACCACTTTTTAAACAACAACAACAACATTATGATTCCACTTCCTCCGATAATACTGCCATTCATGATTTCTTGGAAGGCCAACCACTTCCTTTGTCTCAAGTCAAGATGGGACAACAGTATGAGCTTGTTCTTACTACTTTTACAGGTCAGTTCATCATATCTATCCATTTCTCTCGCCATTTCATATAATTATCTTTTCTTTTTTTAAAAAAACTAATTGGTTGTTTGATTTTATGCTTTAAAACTTGATTGGACATTTTTGGTATGGAAAATCTAATTTAACCCTTGCTTTTTGCTAATTCGTTATCATTATGTCCACCATTACTTTTATAAAATATCTATTACAAGAAGATTATAAGTATCATTGTAGGGTAATCATGGATTATTGATTTGATTCGTAATTGTCTCTACTCCCAATGTTATGTCTCCAACTTACCTTATTTGGCTCCATGTGAAATAAAATTTGAATTTGAGGAATGATCACACAAACTGTCCTCCAACTCCTCCGTTTAGTAATTATTTGGTCATTTTTTTGTTTATGAAAACTAATCATATTTCTTCTTAATTTCTTACGATAATATGCATCTTGTTTTAATACAAAAAAAACAAGAAGTTTTTAAAAAGTACTTTTAGTTTACAAAATTTAGCTTGGGTTGAGATAACAAATCAAGAAACTTAAAAAGGTGGAAAGAGTAATTATAAGTTTAATTTTCAAAAACTAAAAAGTTACCAAATAGGGCCTAAACCGTTTGATAACAATTTCACTTTTAGTTTTATGTTTTTAAAATTTGTGCTTGTTTTCTCTCAAATTTTTTTATGAAAAATTACTATAAACAGAAAAAATATCAAATTATTTACAAATGTAGAAAAATTTCACTATGTATCAGCGATAGACCGCAATAGACTTCTATCTTTGTCTATCGTTCAAGTAATAAAATTCTATCGCGATCTATAGCTGATAGACTGTAAAAAAAATATATATATATTTGTAAATAGTTCGACTCATTTTTCTATATTTCAAAACAACCCTTTCTTTATTGTGATTTTCACATACCTCATCTTAAGGAAACACATGAATTTCTAGTCAAATTTTAGGAGGAAAAAAACAATTTTTTGCAATTTATTTTTTAAAAACGACTTAATTTTCCAAAATATAAGTAAAAAATGATAGCAAGACATATAAACTTATGAAACTGATGAGAAAAAGTAATATTTATAACTTGAATATTGAAAAATAAAAAATTAAAAAATTAAAATGATTATCAAATGATGCATGATGAGCTTAACTATTGCTAGCCTTCCCGATGAATTGTTATAAATTTTGCAACTTTGATGCAATTATATGCTATTGTTATATGTTTGTGGCATACTTAAGTAGATATTAATTTAGTGTAATAATATTATGAAAAAAAAACAATGTAGTAGTTGCAATAGTAGTAGTTGTAGTTGTAGAGAAATTGAGTTTGGGCATTGAAAATTGGGGCGCAGGACTTTATAGATACAGATTAGGAGACGTAGTAGAAGTAGCAGGCTTTCACAACAAGACGCCCAAACTCAACTTTATATGCAGAAGAAAGCTTGTATTAAGCGTAAACATTGACAAGAACACTGAGAAAGATGTTCAATTGGTGGTTGAAAGAGGGTCCCAGTTACTGAGTGATCAAAGTGGAGCCGAACTAGTTGACTTTACTAGCCACGCTGAGGTGTCGTCAGAGCAACCAGGTCACTACGTCATCTACTGGGAAGTCAAAGGAGATGTTAATGACACAGTTTTACAAAACTGTTGCTCAGAAATGGACGGTGCTTTCGTTGACTATGGGTATGTGGTGTCAAGAAAGGCTAACTCAATTGGAGCTCTTGAGCTTAGGATTGTGGAAAGAGGGAGTTTTAATAAGATTTTGGAACATTATATTGGAAATGGAGCTGCACTTAGCCAATTCAAGACCCCAAGATGCACTACCAATCACCTTCTTCTTAATATTCTCAATCTCTCTACTGTCAAATCCTTTTTCAGCACTGCTTACGCCTGACCCTTGCCCTTCTCCTTGTTGTAGTATTTCCCAATTTTGAATCACTTTTGGATCTGAGTTTTTTATTCC

mRNA sequence

GTGTATTCTGTCGTACATTGAATTCGGCGAATGCAACCTTTTGCAGTAGTACATAGTTTAATTTTGTGAGTTTTTTAAACCCATAAAGACGTAACCTAAGCCTAAGCCCCTTTACTAGAAAAACAAACCATATTATTTGTGGGTTGTGGAGAGAGAGAGAGAGAGAGAAAAAAAAAAAAGAAAGTAAAAGAAGGGATATATGTAAAAATGGAAGGTGAGGAAGGAGGAGGTAAGGAATTGATGAAGATTGAGGAATTATGTGAAGAGGAAGAGGTGATTGATTGGTTCGAGCAAGTTTCGTCAGAGGCAGGCGTGGCTCAATCCCGGACTCTCCGTAAGATTCTGCAACAGAATTGTGGTGTTGAGTACCTGAAACAATGGCTTGGAGATGTCAATATCCATGAAATTCCTGATGACTTTACTTTACAATCTCTCTTCACTTCCTTAATCCCCCTCTCTTCCCATTCTGATTTCGAACCCTTCCTCCAACGAATCGCACATGGCGACTCTTCTCCTTTGCTCACTCAACAACCCATCAACACTCTCTCTTTAAGTTCTGGCACTACCGAAGGAAGACAAAAGTACGTTCCCTTTACTCGCCATAGCGCTCAGACTACTCTTCTCATCTTCCGCTTAGCTGCAGCTTATAGATCCAGGGTTTATCCCACAAGGGAAGGAGGGAAGATTCTAGAGTTCATTTACAGTAGCAAACAAACGAAAACAAAAGGAGGAATAACAACGGGAACGGCAACAACACATTATTACGCAAGTGAGGAATTCAAAATCAAACAAGTGAAAACCAAATCCTTCACGTGCAGTCCACAAGAAGTGATATCAGGATGTGACTATAAACAATCAACTTACTGCCATCTCCTACTAGGCCTATTCTATTCAGAAGAAGTAGAGTTCGTGACCTCAACATTTGCGTACACAATAGTCCAAGCTTTAAACCAATTAGAAGAGAGTTGGGAAGAAATGTGTGAAGACATTACAAATGCTACACTTTCCTCAAGAATTGATATACCCAAAATGAGAAAGGGTGTTTTAAAAGTAATGAGTGGGAATCCGGGTTTGGGTTGGAAAATAGGAAGGGTTTGTGAAGAGCTAAAAAGAGAGGATTGGTTGGGTTTAATACCAAAGCTATGGCCAAATTGCAAATATGTATATTCGATAATGACGGGATCAATGCAGCCGTATTTGAAGAAATTGAGACATTATGCTGGGGGATTGCCTTTGGTTAGTGGTGATTATGGATCAACGGAGAGTTGGATTGGGGTTAATGTCGACCCTTATTTGCCACCTGAAAATGTTACTTTTGCGGTCATTCCTACTTTCTCTTATTTTGAATTCATACCACTTTTTAAACAACAACAACAACATTATGATTCCACTTCCTCCGATAATACTGCCATTCATGATTTCTTGGAAGGCCAACCACTTCCTTTGTCTCAAGTCAAGATGGGACAACAGTATGAGCTTGTTCTTACTACTTTTACAGGACTTTATAGATACAGATTAGGAGACGTAGTAGAAGTAGCAGGCTTTCACAACAAGACGCCCAAACTCAACTTTATATGCAGAAGAAAGCTTGTATTAAGCGTAAACATTGACAAGAACACTGAGAAAGATGTTCAATTGGTGGTTGAAAGAGGGTCCCAGTTACTGAGTGATCAAAGTGGAGCCGAACTAGTTGACTTTACTAGCCACGCTGAGGTGTCGTCAGAGCAACCAGGTCACTACGTCATCTACTGGGAAGTCAAAGGAGATGTTAATGACACAGTTTTACAAAACTGTTGCTCAGAAATGGACGGTGCTTTCGTTGACTATGGGTATGTGGTGTCAAGAAAGGCTAACTCAATTGGAGCTCTTGAGCTTAGGATTGTGGAAAGAGGGAGTTTTAATAAGATTTTGGAACATTATATTGGAAATGGAGCTGCACTTAGCCAATTCAAGACCCCAAGATGCACTACCAATCACCTTCTTCTTAATATTCTCAATCTCTCTACTGTCAAATCCTTTTTCAGCACTGCTTACGCCTGACCCTTGCCCTTCTCCTTGTTGTAGTATTTCCCAATTTTGAATCACTTTTGGATCTGAGTTTTTTATTCC

Coding sequence (CDS)

ATGGAAGGTGAGGAAGGAGGAGGTAAGGAATTGATGAAGATTGAGGAATTATGTGAAGAGGAAGAGGTGATTGATTGGTTCGAGCAAGTTTCGTCAGAGGCAGGCGTGGCTCAATCCCGGACTCTCCGTAAGATTCTGCAACAGAATTGTGGTGTTGAGTACCTGAAACAATGGCTTGGAGATGTCAATATCCATGAAATTCCTGATGACTTTACTTTACAATCTCTCTTCACTTCCTTAATCCCCCTCTCTTCCCATTCTGATTTCGAACCCTTCCTCCAACGAATCGCACATGGCGACTCTTCTCCTTTGCTCACTCAACAACCCATCAACACTCTCTCTTTAAGTTCTGGCACTACCGAAGGAAGACAAAAGTACGTTCCCTTTACTCGCCATAGCGCTCAGACTACTCTTCTCATCTTCCGCTTAGCTGCAGCTTATAGATCCAGGGTTTATCCCACAAGGGAAGGAGGGAAGATTCTAGAGTTCATTTACAGTAGCAAACAAACGAAAACAAAAGGAGGAATAACAACGGGAACGGCAACAACACATTATTACGCAAGTGAGGAATTCAAAATCAAACAAGTGAAAACCAAATCCTTCACGTGCAGTCCACAAGAAGTGATATCAGGATGTGACTATAAACAATCAACTTACTGCCATCTCCTACTAGGCCTATTCTATTCAGAAGAAGTAGAGTTCGTGACCTCAACATTTGCGTACACAATAGTCCAAGCTTTAAACCAATTAGAAGAGAGTTGGGAAGAAATGTGTGAAGACATTACAAATGCTACACTTTCCTCAAGAATTGATATACCCAAAATGAGAAAGGGTGTTTTAAAAGTAATGAGTGGGAATCCGGGTTTGGGTTGGAAAATAGGAAGGGTTTGTGAAGAGCTAAAAAGAGAGGATTGGTTGGGTTTAATACCAAAGCTATGGCCAAATTGCAAATATGTATATTCGATAATGACGGGATCAATGCAGCCGTATTTGAAGAAATTGAGACATTATGCTGGGGGATTGCCTTTGGTTAGTGGTGATTATGGATCAACGGAGAGTTGGATTGGGGTTAATGTCGACCCTTATTTGCCACCTGAAAATGTTACTTTTGCGGTCATTCCTACTTTCTCTTATTTTGAATTCATACCACTTTTTAAACAACAACAACAACATTATGATTCCACTTCCTCCGATAATACTGCCATTCATGATTTCTTGGAAGGCCAACCACTTCCTTTGTCTCAAGTCAAGATGGGACAACAGTATGAGCTTGTTCTTACTACTTTTACAGGACTTTATAGATACAGATTAGGAGACGTAGTAGAAGTAGCAGGCTTTCACAACAAGACGCCCAAACTCAACTTTATATGCAGAAGAAAGCTTGTATTAAGCGTAAACATTGACAAGAACACTGAGAAAGATGTTCAATTGGTGGTTGAAAGAGGGTCCCAGTTACTGAGTGATCAAAGTGGAGCCGAACTAGTTGACTTTACTAGCCACGCTGAGGTGTCGTCAGAGCAACCAGGTCACTACGTCATCTACTGGGAAGTCAAAGGAGATGTTAATGACACAGTTTTACAAAACTGTTGCTCAGAAATGGACGGTGCTTTCGTTGACTATGGGTATGTGGTGTCAAGAAAGGCTAACTCAATTGGAGCTCTTGAGCTTAGGATTGTGGAAAGAGGGAGTTTTAATAAGATTTTGGAACATTATATTGGAAATGGAGCTGCACTTAGCCAATTCAAGACCCCAAGATGCACTACCAATCACCTTCTTCTTAATATTCTCAATCTCTCTACTGTCAAATCCTTTTTCAGCACTGCTTACGCCTGA

Protein sequence

MEGEEGGGKELMKIEELCEEEEVIDWFEQVSSEAGVAQSRTLRKILQQNCGVEYLKQWLGDVNIHEIPDDFTLQSLFTSLIPLSSHSDFEPFLQRIAHGDSSPLLTQQPINTLSLSSGTTEGRQKYVPFTRHSAQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQVKTKSFTCSPQEVISGCDYKQSTYCHLLLGLFYSEEVEFVTSTFAYTIVQALNQLEESWEEMCEDITNATLSSRIDIPKMRKGVLKVMSGNPGLGWKIGRVCEELKREDWLGLIPKLWPNCKYVYSIMTGSMQPYLKKLRHYAGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFSYFEFIPLFKQQQQHYDSTSSDNTAIHDFLEGQPLPLSQVKMGQQYELVLTTFTGLYRYRLGDVVEVAGFHNKTPKLNFICRRKLVLSVNIDKNTEKDVQLVVERGSQLLSDQSGAELVDFTSHAEVSSEQPGHYVIYWEVKGDVNDTVLQNCCSEMDGAFVDYGYVVSRKANSIGALELRIVERGSFNKILEHYIGNGAALSQFKTPRCTTNHLLLNILNLSTVKSFFSTAYA
Homology
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BLAST of Lsi10G008740 vs. ExPASy Swiss-Prot
Match: Q9ZNS2 (Indole-3-acetic acid-amido synthetase GH3.10 OS=Arabidopsis thaliana OX=3702 GN=GH3.10 PE=2 SV=1)

HSP 1 Score: 812.0 bits (2096), Expect = 4.6e-234
Identity = 399/590 (67.63%), Postives = 477/590 (80.85%), Query Frame = 0 

Query: 21  EEVIDWFEQVSSEAGVAQSRTLRKILQQNCGVEYLKQWLGDVNIHEIPDDFTLQSLFTSL 80
           ++VI WFE VS  A   QS TLR+IL+ N GVEYL++WLG V++ ++ DD+TL++LFTSL
Sbjct: 9   DDVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKM-DDYTLETLFTSL 68

Query: 81  IPLSSHSDFEPFLQRIAHGDSSPLLTQQPINTLSLSSGTTEGRQKYVPFTRHSAQTTLLI 140
           +P+ SH+D +P++QRIA G++SPLLTQ+PI  LSLSSGTTEGRQKYVPFTRHSAQTTL I
Sbjct: 69  VPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQTTLQI 128

Query: 141 FRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQVKTKS 200
           FRL+AAYRSR YP REGG+ILEFIY+ K+ KT GG+T GTATTHYYASEEFK KQ  TKS
Sbjct: 129 FRLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYYASEEFKTKQETTKS 188

Query: 201 FTCSPQEVISGCDYKQSTYCHLLLGLFYSEEVEFVTSTFAYTIVQALNQLEESWEEMCED 260
           FTCSPQEVISG D+ Q TYCHLLLGL YS +VEFV S F+YTIVQA +  EE W E+C D
Sbjct: 189 FTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQAFSFFEEIWREICAD 248

Query: 261 ITNATLSSRIDIPKMRKGVLKVMSGNPGLGWKIGRVCEELKRE-DWLGLIPKLWPNCKYV 320
           I    LSSRI +PKMRK VL ++  NP L   I  +C EL+    W GLI KLWPN K++
Sbjct: 249 IKEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLGWFGLISKLWPNAKFI 308

Query: 321 YSIMTGSMQPYLKKLRHYAGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFSYF 380
            SIMTGSM PYL KLRHYAGGLPLVS DYGSTESWIGVNVDP+LPPE+V+FAVIPTFSYF
Sbjct: 309 SSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLPPEDVSFAVIPTFSYF 368

Query: 381 EFIPLFKQQQQHYDSTSSDNTAIHDFLEGQPLPLSQVKMGQQYELVLTTFTGLYRYRLGD 440
           EFIPL+++Q Q      SD     DF+E +P+PLSQVK+GQ+YELVLTTFTGLYRYRLGD
Sbjct: 369 EFIPLYRRQNQ------SDICIDGDFVEDKPVPLSQVKLGQEYELVLTTFTGLYRYRLGD 428

Query: 441 VVEVAGFHNKTPKLNFICRRKLVLSVNIDKNTEKDVQLVVERGSQLLSDQSGAELVDFTS 500
           VVEV  FH  TPKL+FI RRKL+L++NIDKNTEKD+Q VV++ SQLLS  + AE+VDFTS
Sbjct: 429 VVEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQLLSRSTRAEVVDFTS 488

Query: 501 HAEVSSEQPGHYVIYWEVKGDVNDTVLQNCCSEMDGAFVDYGYVVSRKANSIGALELRIV 560
           HA+V + +PGHYVIYWE++G+ +D  L+ CC EMD AFVDYGYVVSR+ NSIG LELR+V
Sbjct: 489 HADVIA-RPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSRRMNSIGPLELRVV 548

Query: 561 ERGSFNKILEHYIGNGAALSQFKTPRCTTNHLLLNILNLSTVKSFFSTAY 610
           ERG+F K+ E  +G    L+QFKTPRCTTN ++L+ILN ST+K F S+AY
Sbjct: 549 ERGTFGKVAERCVGKCGGLNQFKTPRCTTNSVMLDILNDSTIKRFRSSAY 590

[Close]
BLAST of Lsi10G008740 vs. ExPASy Swiss-Prot
Match: A0A1J6KGJ9 (Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 PE=1 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 3.7e-175
Identity = 310/603 (51.41%), Postives = 427/603 (70.81%), Query Frame = 0 

Query: 9   KELMKIEELCEEEEVIDWFEQVSSEAGVAQSRTLRKILQQNCGVEYLKQWLGDVNIHEIP 68
           K +++  E  + EEVI+ FE ++ +AG  Q  TL+KIL++N G EYL+QW     ++   
Sbjct: 2   KMVVEKTEKFDPEEVIEEFEVLTKDAGKIQEETLQKILEENGGTEYLQQW----GLNGKT 61

Query: 69  DDFTLQSLFTSLIPLSSHSDFEPFLQRIAHGDSSPLLTQQPINTLSLSSGTTEGRQKYVP 128
           D  +    F + IP+ +H D EP++ RIA GD SP+LT +PI T+SLSSGTT+G+ K+VP
Sbjct: 62  DSLS----FKNCIPIVTHKDLEPYIHRIADGDLSPILTGKPITTISLSSGTTQGKPKFVP 121

Query: 129 FTRHSAQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYAS 188
           F     ++T+ IF+ +  +R+R +P    GK L+FIY SKQ KTKGG+  GTATT+ Y +
Sbjct: 122 FNEELMESTMQIFKTSFVFRNREFPV-VNGKALQFIYGSKQFKTKGGLAAGTATTNVYRN 181

Query: 189 EEFKIKQVKTKSFTCSPQEVISGCDYKQSTYCHLLLGLFYSEEVEFVTSTFAYTIVQALN 248
            +FK      ++  CSP EVI G D++QS YCHLL GL + +EV+ V+STFA++IV A  
Sbjct: 182 AQFKKTMKAMQTPCCSPDEVIFGPDFQQSLYCHLLCGLIFRDEVQVVSSTFAHSIVHAFR 241

Query: 249 QLEESWEEMCEDITNATLSSRIDIPKMRKGVLKVMSGNPGLGWKIGRVCEELKREDWLGL 308
             E+ W+E+  +I    LSSR+ +P MR  + K++  +P L   I   C  L   +W GL
Sbjct: 242 NFEQIWQELVTNIREGVLSSRVIVPSMRAAMSKLLKPDPELADTIFNKCSRL--SNWYGL 301

Query: 309 IPKLWPNCKYVYSIMTGSMQPYLKKLRHYAGGLPLVSGDYGSTESWIGVNVDPYLPPENV 368
           IP+L+PN +Y+Y IMTGSM+PYLKKLRHYAG LPL+S DYGS+E WIG NV+P LPPE V
Sbjct: 302 IPELFPNTRYIYGIMTGSMEPYLKKLRHYAGDLPLLSADYGSSEGWIGANVNPELPPELV 361

Query: 369 TFAVIPTFSYFEFIPLFKQQQQHYDSTSSDNTAIHDFLEGQPLPLSQVKMGQQYELVLTT 428
           T+AV+P   YFEFIPL +                 D LE  P+ L++VK+G++YE+V+T 
Sbjct: 362 TYAVLPNIGYFEFIPLMENL---------------DGLEPMPVGLTEVKLGEEYEIVVTN 421

Query: 429 FTGLYRYRLGDVVEVAGFHNKTPKLNFICRRKLVLSVNIDKNTEKDVQLVVERGSQLLSD 488
           F GLYRYRLGDVV++ GFHN TP+L FICRR L+LS+NIDKNTEKD+QL VE  +++LSD
Sbjct: 422 FAGLYRYRLGDVVKIKGFHNGTPELQFICRRNLLLSINIDKNTEKDLQLAVEAAAKILSD 481

Query: 489 QSGAELVDFTSHAEVSSEQPGHYVIYWEVKGDVNDTVLQNCCSEMDGAFVDYGYVVSRKA 548
           +   E+VDFTSH  VS++ PGHYVI+WE+ G+ ++ +L+ CC+ +D +FVD GYV SRK 
Sbjct: 482 EK-LEVVDFTSHVNVSAD-PGHYVIFWELNGEASEEILKECCNCLDKSFVDAGYVGSRKV 541

Query: 549 NSIGALELRIVERGSFNKILEHYIGNGAALSQFKTPRCT--TNHLLLNILNLSTVKSFFS 608
           ++IGALELRIV+RG+F+KIL+H++G GAA+SQFKTPRC   TN  +L IL+ + V+S+FS
Sbjct: 542 HAIGALELRIVKRGTFHKILDHFVGLGAAVSQFKTPRCVGPTNLSVLQILSSNVVESYFS 576

Query: 609 TAY 610
           TA+
Sbjct: 602 TAF 576

[Close]
BLAST of Lsi10G008740 vs. ExPASy Swiss-Prot
Match: A0A314KSQ4 (Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 PE=1 SV=2)

HSP 1 Score: 604.7 bits (1558), Expect = 1.1e-171
Identity = 308/599 (51.42%), Postives = 417/599 (69.62%), Query Frame = 0 

Query: 13  KIEELCEEEEVIDWFEQVSSEAGVAQSRTLRKILQQNCGVEYLKQWLGDVNIHEIPDDFT 72
           KIE+  ++E+VI+ FE ++ +AG  Q  TL+KIL+QN G EYL+ W   +N    P    
Sbjct: 7   KIEKKFDQEKVIEEFEDLTKDAGKIQEETLKKILEQNGGTEYLQLW--GLNGRTDP---- 66

Query: 73  LQSLFTSLIPLSSHSDFEPFLQRIAHGDSSPLLTQQPINTLSLSSGTTEGRQKYVPFTRH 132
               F + +P+ +H+D EP++QRIA GD SP+LT +PI T+SLSSGTT+G+ K+VPF   
Sbjct: 67  --QTFKNCVPIVTHNDLEPYIQRIADGDLSPILTGKPIETISLSSGTTQGKPKFVPFNDE 126

Query: 133 SAQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFK 192
             ++T+ IF+ + A+R+R +P    GK L+FIYSSKQ KTKGG+  GTATT+ Y + +FK
Sbjct: 127 LMESTMQIFKTSFAFRNREFPI-GNGKALQFIYSSKQFKTKGGLAAGTATTNVYRNAQFK 186

Query: 193 IKQVKTKSFTCSPQEVISGCDYKQSTYCHLLLGLFYSEEVEFVTSTFAYTIVQALNQLEE 252
                  +  CSP EVI G D+ QS YCHLL GL + +EV+ V+STFA++IV A    E+
Sbjct: 187 KTMKAMCTPCCSPDEVIFGPDFHQSLYCHLLCGLIFHDEVQVVSSTFAHSIVHAFRTFEQ 246

Query: 253 SWEEMCEDITNATLSSRIDIPKMRKGVLKVMSGNPGLGWKIGRVCEELKREDWLGLIPKL 312
            WE +  DI    LSSR+ +P +R  + K++  +P L   I   C  L   +W GLIP L
Sbjct: 247 VWEALVVDIREGVLSSRVTVPSIRLAMSKLLKPDPELADTIYNKCSRL--SNWYGLIPDL 306

Query: 313 WPNCKYVYSIMTGSMQPYLKKLRHYAGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAV 372
           +PN +Y+Y IMTGSM+PYLKKLRHYAG LPL+S DYGS+E W+GVNV+P LPPE VT+AV
Sbjct: 307 FPNTRYIYGIMTGSMEPYLKKLRHYAGELPLLSADYGSSEGWVGVNVNPKLPPELVTYAV 366

Query: 373 IPTFSYFEFIPLFKQQQQHYDSTSSDNTAIHDFLEGQPLPLSQVKMGQQYELVLTTFTGL 432
           +P   YFEFIPL         + S             P+ L++VK+G++YE+V T F GL
Sbjct: 367 LPNIGYFEFIPLGGNLNGIEQANS-------------PVGLTEVKLGEEYEVVFTNFAGL 426

Query: 433 YRYRLGDVVEVAGFHNKTPKLNFICRRKLVLSVNIDKNTEKDVQLVVERGSQLLSDQSGA 492
           YRYRLGDVV+V GFHN TP+L F+CR  L+LS+NIDKNTEKD+QL VE  ++ L D+   
Sbjct: 427 YRYRLGDVVKVKGFHNGTPELQFVCRSNLLLSINIDKNTEKDLQLAVEAAAKRLVDEK-L 486

Query: 493 ELVDFTSHAEVSSEQPGHYVIYWEVKGDVNDTVLQNCCSEMDGAFVDYGYVVSRKANSIG 552
           E+VDFTSH  VS++ PGHYVI+WE+ G+  D +LQ+CC+ +D +F+D GYV SRK N+IG
Sbjct: 487 EVVDFTSHVNVSAD-PGHYVIFWELSGEATDEMLQDCCNCLDRSFIDAGYVSSRKVNAIG 546

Query: 553 ALELRIVERGSFNKILEHYIGNGAALSQFKTPRCT--TNHLLLNILNLSTVKSFFSTAY 610
           ALELRIV+RG+F+KIL+H++G G A+SQFKTPRC    N  LL IL+ + V+++ STA+
Sbjct: 547 ALELRIVKRGTFHKILDHFVGLGGAVSQFKTPRCVGPKNSSLLQILSSNVVETYVSTAF 579

[Close]
BLAST of Lsi10G008740 vs. ExPASy Swiss-Prot
Match: Q6I581 (Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 1.4e-166
Identity = 301/596 (50.50%), Postives = 404/596 (67.79%), Query Frame = 0 

Query: 17  LCEEEEVIDWFEQVSSEAGVAQSRTLRKILQQNCGVEYLKQW-LGDVNIHEIPDDFTLQS 76
           +C  EE I+ FE ++ +A   Q  TL+KIL+ N   EYL+ + LG           T   
Sbjct: 3   ICSCEETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNFGLG---------GRTDAE 62

Query: 77  LFTSLIPLSSHSDFEPFLQRIAHGDSSPLLTQQPINTLSLSSGTTEGRQKYVPFTRHSAQ 136
            + S IPL  H+D EP++QRI  GD+SP++T +PI  LSLSSGTT G+ K++PF     +
Sbjct: 63  SYKSCIPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKPKFIPFNDELLE 122

Query: 137 TTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQ 196
           TTL I+R + A+R+R YP  + GK L+F+Y SKQ  TKGGI   TATT+ Y  + +K   
Sbjct: 123 TTLQIYRTSYAFRNREYPIGQ-GKALQFVYGSKQVITKGGILATTATTNLYRRQRYKEGM 182

Query: 197 VKTKSFTCSPQEVISGCDYKQSTYCHLLLGLFYSEEVEFVTSTFAYTIVQALNQLEESWE 256
              +S  CSP EVI G D+ QS YCHLL GL YSEEV  V STFA+++V A    EE WE
Sbjct: 183 KDIQSQCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAHSLVHAFQTFEEVWE 242

Query: 257 EMCEDITNATLSSRIDIPKMRKGVLKVMSGNPGLGWKIGRVCEELKREDWLGLIPKLWPN 316
           ++C DI +  LS ++  P +R+ V K++  NP L   I + C  L   +W G+IP LWPN
Sbjct: 243 DLCTDIRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIGL--SNWYGVIPALWPN 302

Query: 317 CKYVYSIMTGSMQPYLKKLRHYAGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPT 376
            KYVY IMTGSM+PYLKKLRHYAG LPL+S DYG++E W+G N+DP +PPE VT+AV+P 
Sbjct: 303 AKYVYGIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNIDPTVPPEQVTYAVLPQ 362

Query: 377 FSYFEFIPLFKQQQQHYDSTSSDNTAIHDFLEGQPLPLSQVKMGQQYELVLTTFTGLYRY 436
             YFEFIPL K   +      ++N+A   ++E  P+ L++V++G+ YE+V+T F GLYRY
Sbjct: 363 VGYFEFIPLEKPIGE-----ETENSASIHYIESDPVGLTEVEVGKIYEVVITNFAGLYRY 422

Query: 437 RLGDVVEVAGFHNKTPKLNFICRRKLVLSVNIDKNTEKDVQLVVERGSQLLSDQSGAELV 496
           RLGDVV++A FHN TP+L FICRR LVLS+NIDKNTEKD+QL VE  S+ L  +   E++
Sbjct: 423 RLGDVVKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVEEASKFLEGEK-LEVM 482

Query: 497 DFTSHAEVSSEQPGHYVIYWEVKGDVNDTVLQNCCSEMDGAFVDYGYVVSRKANSIGALE 556
           DFTS  E SS+ PG YVI+WE+ GD +D VL +C + +D AF+D GY  SRK  +IG LE
Sbjct: 483 DFTSFVERSSD-PGRYVIFWELSGDASDEVLSSCANALDLAFIDAGYTGSRKIKTIGPLE 542

Query: 557 LRIVERGSFNKILEHYIGNGAALSQFKTPRCT--TNHLLLNILNLSTVKSFFSTAY 610
           LRI+ +G+F +IL+H++  G A+SQFKTPR    +N  +L IL+ +  +S+FSTAY
Sbjct: 543 LRILRKGTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQILSRNVTQSYFSTAY 579

[Close]
BLAST of Lsi10G008740 vs. ExPASy Swiss-Prot
Match: Q9SKE2 (Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 PE=1 SV=2)

HSP 1 Score: 549.7 bits (1415), Expect = 4.3e-155
Identity = 279/596 (46.81%), Postives = 399/596 (66.95%), Query Frame = 0 

Query: 16  ELCEEEEVIDWFEQVSSEAGVAQSRTLRKILQQNCGVEYLKQWLGDVNIHEIPDDFTLQS 75
           E  +   VID F++++  A   Q +TL++IL +N    YL+    + N  + P++     
Sbjct: 6   ETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATD-PEE----- 65

Query: 76  LFTSLIPLSSHSDFEPFLQRIAHGDSSPLLTQQPINTLSLSSGTTEGRQKYVPFTRHSAQ 135
            F S++PL +  + EP+++R+  GD+SP+LT  P+  +SLSSGT++GR K++PFT    +
Sbjct: 66  AFKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELME 125

Query: 136 TTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQ 195
            TL +FR A A+R+R +P  + GK L+FI+SSKQ  + GG+  GTATT+ Y +  FK   
Sbjct: 126 NTLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGM 185

Query: 196 VKTKSFTCSPQEVISGCDYKQSTYCHLLLGLFYSEEVEFVTSTFAYTIVQALNQLEESWE 255
               S +CSP EVI   D  Q+ YCHLL G+ + ++V++V + FA+ +V A    E+ WE
Sbjct: 186 KSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWE 245

Query: 256 EMCEDITNATLSSRIDIPKMRKGVLKVMSGNPGLGWKIGRVCEELKREDWLGLIPKLWPN 315
           E+  DI +  LS+RI +P +R  + K+++ NP L   I   C  L   +W GLIP L+PN
Sbjct: 246 EIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPN 305

Query: 316 CKYVYSIMTGSMQPYLKKLRHYAGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPT 375
            KYVY IMTGSM+PY+ KLRHYAG LPLVS DYGS+E WI  NV P L PE  TFAVIP 
Sbjct: 306 AKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPN 365

Query: 376 FSYFEFIPLFKQQQQHYDSTSSDNTAIHDFLEGQPLPLSQVKMGQQYELVLTTFTGLYRY 435
             YFEF+P+ +  +                 E +P+ L+QVK+G++YE+V+T + GLYRY
Sbjct: 366 LGYFEFLPVSETGEG----------------EEKPVGLTQVKIGEEYEVVITNYAGLYRY 425

Query: 436 RLGDVVEVAGFHNKTPKLNFICRRKLVLSVNIDKNTEKDVQLVVERGSQLLSDQSGAELV 495
           RLGDVV+V GF+N TP+L FICRR L+LS+NIDKNTE+D+QL VE  ++ LS++   E++
Sbjct: 426 RLGDVVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK-IEVI 485

Query: 496 DFTSHAEVSSEQPGHYVIYWEVKGDVNDTVLQNCCSEMDGAFVDYGYVVSRKANSIGALE 555
           DF+S+ +VS++ PGHY I+WE+ G+ N+ VLQ+CC+ +D AF+D GYV SRK  +IGALE
Sbjct: 486 DFSSYIDVSTD-PGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALE 545

Query: 556 LRIVERGSFNKILEHYIGNGAALSQFKTPRCT--TNHLLLNILNLSTVKSFFSTAY 610
           LR+V +G+F KI EH++G G++  QFK PRC   +N  +L IL  + V S+FSTA+
Sbjct: 546 LRVVAKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

[Close]
BLAST of Lsi10G008740 vs. ExPASy TrEMBL
Match: A0A5D3B9T4 (Jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold464G00300 PE=3 SV=1)

HSP 1 Score: 1074.3 bits (2777), Expect = 2.3e-310
Identity = 536/600 (89.33%), Postives = 564/600 (94.00%), Query Frame = 0 

Query: 12  MKIEELCEEEE-VIDWFEQVSSEAGVAQSRTLRKILQQNCGVEYLKQWLGDVNIHEIPDD 71
           MKI+ELCEEEE +IDWFEQVSSEA VAQSRTLRKILQQN GVEYLK+W+GDVNIHEIPDD
Sbjct: 1   MKIDELCEEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDD 60

Query: 72  FTLQSLFTSLIPLSSHSDFEPFLQRIAHGDSSPLLTQQPINTLSLSSGTTEGRQKYVPFT 131
           FTLQS+FTSLIPLSSH+ FEPFLQRIA GDSSPLLTQQPI TLSLSSGTTEGRQKYVPFT
Sbjct: 61  FTLQSIFTSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFT 120

Query: 132 RHSAQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEE 191
            HSA TTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEE
Sbjct: 121 PHSAHTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEE 180

Query: 192 FKIKQVKTKSFTCSPQEVISGCDYKQSTYCHLLLGLFYSEEVEFVTSTFAYTIVQALNQL 251
           FKIKQ+KTKSFTCSPQEVISGCDYKQSTYCHLLLGL YSEEVEFVTSTFAYTIVQA NQL
Sbjct: 181 FKIKQLKTKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQAFNQL 240

Query: 252 EESWEEMCEDITNATLSSRIDIPKMRKGVLKVMSGNPGLGWKIGRVCEELKREDWLGLIP 311
           EESWEE+  DI++ATLSSRIDIP++RK VLKVMS  P LGWKI RVCEELK+E WLGLIP
Sbjct: 241 EESWEEIIHDISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKKEGWLGLIP 300

Query: 312 KLWPNCKYVYSIMTGSMQPYLKKLRHYAGGLPLVSGDYGSTESWIGVNVDPYLPPENVTF 371
           KLWPNCKYVYSIMTGSMQPYLKKLRHY GGLPLVSGDYGSTESWIGVNVDPYLPPENVTF
Sbjct: 301 KLWPNCKYVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPPENVTF 360

Query: 372 AVIPTFSYFEFIPLFKQQQQHYDSTSSDNTAIHDFLEGQPLPLSQVKMGQQYELVLTTFT 431
           AVIPTFSYFEFIPLFKQ    + +TSS +TAIHDFLEGQP+ LS+VK+GQQYELVLTTFT
Sbjct: 361 AVIPTFSYFEFIPLFKQ----HATTSSTDTAIHDFLEGQPIALSEVKIGQQYELVLTTFT 420

Query: 432 GLYRYRLGDVVEVAGFHNKTPKLNFICRRKLVLSVNIDKNTEKDVQLVVERGSQLLSDQS 491
           GLYR RLGDVVEVAGFHNKTPKLNFICRRKLVL+VNIDKNTEKDVQL VERGSQL+S  S
Sbjct: 421 GLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQLMSQLS 480

Query: 492 GAELVDFTSHAEVSSEQPGHYVIYWEVKGDVNDTVLQNCCSEMDGAFVDYGYVVSRKANS 551
           GAELVDFTS+AE+S+ QPGHYVI+WEVKGDVNDTVL  CCSEMD AFVDYGYVVSRKANS
Sbjct: 481 GAELVDFTSYAELSN-QPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVVSRKANS 540

Query: 552 IGALELRIVERGSFNKILEHYIGNGAALSQFKTPRCTTNHLLLNILNLSTVKSFFSTAYA 611
           IG LELRIVERGSFNKILE+YIGNGAALSQFKTPRCTTNH LLNILNLST++SFFSTAYA
Sbjct: 541 IGPLELRIVERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSFFSTAYA 595

[Close]
BLAST of Lsi10G008740 vs. ExPASy TrEMBL
Match: A0A1S3BIL6 (jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490483 PE=3 SV=1)

HSP 1 Score: 1074.3 bits (2777), Expect = 2.3e-310
Identity = 536/600 (89.33%), Postives = 564/600 (94.00%), Query Frame = 0 

Query: 12  MKIEELCEEEE-VIDWFEQVSSEAGVAQSRTLRKILQQNCGVEYLKQWLGDVNIHEIPDD 71
           MKI+ELCEEEE +IDWFEQVSSEA VAQSRTLRKILQQN GVEYLK+W+GDVNIHEIPDD
Sbjct: 1   MKIDELCEEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDD 60

Query: 72  FTLQSLFTSLIPLSSHSDFEPFLQRIAHGDSSPLLTQQPINTLSLSSGTTEGRQKYVPFT 131
           FTLQS+FTSLIPLSSH+ FEPFLQRIA GDSSPLLTQQPI TLSLSSGTTEGRQKYVPFT
Sbjct: 61  FTLQSIFTSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFT 120

Query: 132 RHSAQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEE 191
            HSA TTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEE
Sbjct: 121 PHSAHTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEE 180

Query: 192 FKIKQVKTKSFTCSPQEVISGCDYKQSTYCHLLLGLFYSEEVEFVTSTFAYTIVQALNQL 251
           FKIKQ+KTKSFTCSPQEVISGCDYKQSTYCHLLLGL YSEEVEFVTSTFAYTIVQA NQL
Sbjct: 181 FKIKQLKTKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQAFNQL 240

Query: 252 EESWEEMCEDITNATLSSRIDIPKMRKGVLKVMSGNPGLGWKIGRVCEELKREDWLGLIP 311
           EESWEE+  DI++ATLSSRIDIP++RK VLKVMS  P LGWKI RVCEELK+E WLGLIP
Sbjct: 241 EESWEEIIHDISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKKEGWLGLIP 300

Query: 312 KLWPNCKYVYSIMTGSMQPYLKKLRHYAGGLPLVSGDYGSTESWIGVNVDPYLPPENVTF 371
           KLWPNCKYVYSIMTGSMQPYLKKLRHY GGLPLVSGDYGSTESWIGVNVDPYLPPENVTF
Sbjct: 301 KLWPNCKYVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPPENVTF 360

Query: 372 AVIPTFSYFEFIPLFKQQQQHYDSTSSDNTAIHDFLEGQPLPLSQVKMGQQYELVLTTFT 431
           AVIPTFSYFEFIPLFKQ    + +TSS +TAIHDFLEGQP+ LS+VK+GQQYELVLTTFT
Sbjct: 361 AVIPTFSYFEFIPLFKQ----HATTSSTDTAIHDFLEGQPIALSEVKIGQQYELVLTTFT 420

Query: 432 GLYRYRLGDVVEVAGFHNKTPKLNFICRRKLVLSVNIDKNTEKDVQLVVERGSQLLSDQS 491
           GLYR RLGDVVEVAGFHNKTPKLNFICRRKLVL+VNIDKNTEKDVQL VERGSQL+S  S
Sbjct: 421 GLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQLMSQLS 480

Query: 492 GAELVDFTSHAEVSSEQPGHYVIYWEVKGDVNDTVLQNCCSEMDGAFVDYGYVVSRKANS 551
           GAELVDFTS+AE+S+ QPGHYVI+WEVKGDVNDTVL  CCSEMD AFVDYGYVVSRKANS
Sbjct: 481 GAELVDFTSYAELSN-QPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVVSRKANS 540

Query: 552 IGALELRIVERGSFNKILEHYIGNGAALSQFKTPRCTTNHLLLNILNLSTVKSFFSTAYA 611
           IG LELRIVERGSFNKILE+YIGNGAALSQFKTPRCTTNH LLNILNLST++SFFSTAYA
Sbjct: 541 IGPLELRIVERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSFFSTAYA 595

[Close]
BLAST of Lsi10G008740 vs. ExPASy TrEMBL
Match: A0A0A0KE57 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G124120 PE=3 SV=1)

HSP 1 Score: 1050.0 bits (2714), Expect = 3.7e-303
Identity = 521/591 (88.16%), Postives = 551/591 (93.23%), Query Frame = 0 

Query: 20  EEEVIDWFEQVSSEAGVAQSRTLRKILQQNCGVEYLKQWLGDVNIHEIPDDFTLQSLFTS 79
           EE++I WFEQVSSEA +AQSRTLRKILQQN GVEYLK+W+GDVNIHEIPDDFTL S+FTS
Sbjct: 4   EEDIIGWFEQVSSEAALAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDDFTLHSIFTS 63

Query: 80  LIPLSSHSDFEPFLQRIAHGDSSPLLTQQPINTLSLSSGTTEGRQKYVPFTRHSAQTTLL 139
            IPLSSH+ FEPFLQRIA GDSSPLLTQQPI TLSLSSGTTEGRQKYVPFT HSAQTTLL
Sbjct: 64  SIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHSAQTTLL 123

Query: 140 IFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQVKTK 199
           IFRLAAAYRSRVYP R+GGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQ+KTK
Sbjct: 124 IFRLAAAYRSRVYPIRDGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLKTK 183

Query: 200 SFTCSPQEVISGCDYKQSTYCHLLLGLFYSEEVEFVTSTFAYTIVQALNQLEESWEEMCE 259
           SFTCSPQEVI+GCDYKQSTYCHLLLGL YSEEVEFVTSTFAYTIVQA NQLEESWEEM  
Sbjct: 184 SFTCSPQEVITGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQAFNQLEESWEEMIH 243

Query: 260 DITNATLSSRIDIPKMRKGVLKVMSGNPGLGWKIGRVCEELKREDWLGLIPKLWPNCKYV 319
           D+++ATLSSRIDIP++RK VLKVMS  P LGWKI RVCEEL +E WLGLIPKLWPNCKYV
Sbjct: 244 DLSHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELMKEGWLGLIPKLWPNCKYV 303

Query: 320 YSIMTGSMQPYLKKLRHYAGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFSYF 379
           YSIMTGSMQPYLKKLRHY GGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFSYF
Sbjct: 304 YSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFSYF 363

Query: 380 EFIPLFKQQQQHYDSTSSDNTAIHDFLEGQPLPLSQVKMGQQYELVLTTFTGLYRYRLGD 439
           EFIPLFKQ    + +TSS +TAIHDFLEGQP+ LS+VK+GQQYELVLTTFTGLYR RLGD
Sbjct: 364 EFIPLFKQ----HATTSSADTAIHDFLEGQPIALSEVKIGQQYELVLTTFTGLYRCRLGD 423

Query: 440 VVEVAGFHNKTPKLNFICRRKLVLSVNIDKNTEKDVQLVVERGSQLLSDQSGAELVDFTS 499
           VVEVAGFHNKTPKLNFICRRKLVL+VNIDKNTEKDVQL VERGSQL+S  SGAELVDFTS
Sbjct: 424 VVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQLMSQLSGAELVDFTS 483

Query: 500 HAEVSSEQPGHYVIYWEVKGDVNDTVLQNCCSEMDGAFVDYGYVVSRKANSIGALELRIV 559
           +AE+S+ QPGHYVI+WE+KGDVND VL  CCSEMD AFVDYGYVVSRKANSIG LELRIV
Sbjct: 484 YAELSN-QPGHYVIFWELKGDVNDKVLGMCCSEMDAAFVDYGYVVSRKANSIGPLELRIV 543

Query: 560 ERGSFNKILEHYIGNGAALSQFKTPRCTTNHLLLNILNLSTVKSFFSTAYA 611
           ERGSFNKILEHYIGNGAALSQFKTPRCTTNH LLNILNLST+KSFFSTAYA
Sbjct: 544 ERGSFNKILEHYIGNGAALSQFKTPRCTTNHFLLNILNLSTLKSFFSTAYA 589

[Close]
BLAST of Lsi10G008740 vs. ExPASy TrEMBL
Match: A0A5A7VB01 (Jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold301G00260 PE=3 SV=1)

HSP 1 Score: 1044.6 bits (2700), Expect = 1.6e-301
Identity = 527/605 (87.11%), Postives = 557/605 (92.07%), Query Frame = 0 

Query: 12  MKIEELCEEEE-VIDWFEQVSSEAGVAQSRTLRKILQQNCGVEYLKQWLGDVNIHEIPDD 71
           MKI+ELCEEEE +IDWFEQVSSEA VAQSRTLRKILQQN GVEYLK+W+GDVNIHEIPDD
Sbjct: 1   MKIDELCEEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDD 60

Query: 72  FTLQSLFTSLIPLSSHSDFEPFLQRIAHGDSSPLLTQQPINTLSLSSGTTEGRQKYVPFT 131
           FTLQS+FTSLIPLSSH+ FEPFLQRIA GDSSPLLTQQPI TLSLSSGTTEGRQKYVPFT
Sbjct: 61  FTLQSIFTSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFT 120

Query: 132 RHSAQTTLLIFRLAAAYRSRVYPTREG-----GKILEFIYSSKQTKTKGGITTGTATTHY 191
            HSAQTTLLIFRLAAAYRS     ++        ILEFIYSSKQTKTKGGITTGTATTHY
Sbjct: 121 PHSAQTTLLIFRLAAAYRSTNITQKQHLLTIYLLILEFIYSSKQTKTKGGITTGTATTHY 180

Query: 192 YASEEFKIKQVKTKSFTCSPQEVISGCDYKQSTYCHLLLGLFYSEEVEFVTSTFAYTIVQ 251
           YASEEFKIKQ+KTKSFTCSPQEVISGCDYKQSTYCHLLLGL YSEEVEFVTSTFAYTIVQ
Sbjct: 181 YASEEFKIKQLKTKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQ 240

Query: 252 ALNQLEESWEEMCEDITNATLSSRIDIPKMRKGVLKVMSGNPGLGWKIGRVCEELKREDW 311
           A NQLEESWEE+  DI++ATLSSRIDIP++RK VLKVMS  P LGWKI RVCEELK+E W
Sbjct: 241 AFNQLEESWEEIIHDISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKKEGW 300

Query: 312 LGLIPKLWPNCKYVYSIMTGSMQPYLKKLRHYAGGLPLVSGDYGSTESWIGVNVDPYLPP 371
           LGLIPKLWPNCKYVYSIMTGSMQPYLKKLRHY GGLPLVSGDYGSTESWIGVNVDPYLPP
Sbjct: 301 LGLIPKLWPNCKYVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPP 360

Query: 372 ENVTFAVIPTFSYFEFIPLFKQQQQHYDSTSSDNTAIHDFLEGQPLPLSQVKMGQQYELV 431
           ENVTFAVIPTFSYFEFIPLFKQ    + +TSS +TAIHDFLEGQP+ LS+VK+GQQYELV
Sbjct: 361 ENVTFAVIPTFSYFEFIPLFKQ----HATTSSTDTAIHDFLEGQPIALSEVKIGQQYELV 420

Query: 432 LTTFTGLYRYRLGDVVEVAGFHNKTPKLNFICRRKLVLSVNIDKNTEKDVQLVVERGSQL 491
           LTTFTGLYR RLGDVVEVAGFHNKTPKLNFICRRKLVL+VNIDKNTEKDVQL VERGSQL
Sbjct: 421 LTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQL 480

Query: 492 LSDQSGAELVDFTSHAEVSSEQPGHYVIYWEVKGDVNDTVLQNCCSEMDGAFVDYGYVVS 551
           +S  SGAELVDFTS+AE+S+ QPGHYVI+WEVKGDVNDTVL  CCSEMD AFVDYGYVVS
Sbjct: 481 MSQLSGAELVDFTSYAELSN-QPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVVS 540

Query: 552 RKANSIGALELRIVERGSFNKILEHYIGNGAALSQFKTPRCTTNHLLLNILNLSTVKSFF 611
           RKANSIG LELRIVERGSFNKILE+YIGNGAALSQFKTPRCTTNH LLNILNLST++SFF
Sbjct: 541 RKANSIGPLELRIVERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSFF 600

[Close]
BLAST of Lsi10G008740 vs. ExPASy TrEMBL
Match: A0A6J1FRQ1 (indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita moschata OX=3662 GN=LOC111448207 PE=3 SV=1)

HSP 1 Score: 1019.2 bits (2634), Expect = 7.0e-294
Identity = 499/589 (84.72%), Postives = 546/589 (92.70%), Query Frame = 0 

Query: 21  EEVIDWFEQVSSEAGVAQSRTLRKILQQNCGVEYLKQWLGDVNIHEIPDDFTLQSLFTSL 80
           E+++DWFE+VSSEAGVAQ RTLR+IL+QNCGVEYL QWLGDVNIH+IPDDF L+SLFTSL
Sbjct: 6   EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65

Query: 81  IPLSSHSDFEPFLQRIAHGDSSPLLTQQPINTLSLSSGTTEGRQKYVPFTRHSAQTTLLI 140
           IPLSSH+DFEPFLQRIA GDSSPLLTQQPI TLSLSSGTTEGRQKYVPFT HSAQTTLLI
Sbjct: 66  IPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125

Query: 141 FRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQVKTKS 200
           FR+AAAYRSRVYPTREGG+ILEFIYSSKQTKTKGGITTGTATTHYYASE+FKIKQ+KTKS
Sbjct: 126 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 185

Query: 201 FTCSPQEVISGCDYKQSTYCHLLLGLFYSEEVEFVTSTFAYTIVQALNQLEESWEEMCED 260
           FTCSPQEVISG DYKQSTYCHLLLGLF+S++VEFVTSTFAYTIVQA N+LE+ W ++C D
Sbjct: 186 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 245

Query: 261 ITNATLSSRIDIPKMRKGVLKVMSGNPGLGWKIGRVCEELKREDWLGLIPKLWPNCKYVY 320
           IT+ATL SRI +P++RK VL ++S NP LG KIG VCEEL+R+DWL L+PKLWPNCKYVY
Sbjct: 246 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKYVY 305

Query: 321 SIMTGSMQPYLKKLRHYAGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFSYFE 380
           SIMTGSMQPYLKKLR YAG LPLVSGDYGSTESWIGVNVDP LPPENVTFAV+PTFSYFE
Sbjct: 306 SIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYFE 365

Query: 381 FIPLFKQQQQHYDSTSSDNTAIHDFLEGQPLPLSQVKMGQQYELVLTTFTGLYRYRLGDV 440
           FIPLF+Q QQH D  SS   A  DFLEGQPLPLSQVK+GQQYELVLTTFTGLYR RLGDV
Sbjct: 366 FIPLFRQHQQH-DCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGDV 425

Query: 441 VEVAGFHNKTPKLNFICRRKLVLSVNIDKNTEKDVQLVVERGSQLLSDQSGAELVDFTSH 500
           VEVAGFHNKTPKLNFICRRKLVL+VNIDKNTEKD+QLVVERGSQLL+DQSGAELVDFTSH
Sbjct: 426 VEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSH 485

Query: 501 AEVSSEQPGHYVIYWEVKGDVNDTVLQNCCSEMDGAFVDYGYVVSRKANSIGALELRIVE 560
           A++++ QPGHYVI+WEVKG V D VL +CCSEMD AFVDYGYVVSR++N+IG LELRIVE
Sbjct: 486 ADLTN-QPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVE 545

Query: 561 RGSFNKILEHYIGNGAALSQFKTPRCTTNHLLLNILNLSTVKSFFSTAY 610
           RGSFNKI+EHYIGNGAALSQFKTPRCTTN +LL ILNLSTVK+F STAY
Sbjct: 546 RGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 592

[Close]
BLAST of Lsi10G008740 vs. NCBI nr
Match: XP_038900326.1 (indole-3-acetic acid-amido synthetase GH3.10 [Benincasa hispida])

HSP 1 Score: 1107.0 bits (2862), Expect = 0.0e+00
Identity = 556/611 (91.00%), Postives = 578/611 (94.60%), Query Frame = 0 

Query: 1   MEGE-EGGGKELMKIEELCEEEEVIDWFEQVSSEAGVAQSRTLRKILQQNCGVEYLKQWL 60
           MEGE   GG+ LMKI+ELC EE+VIDWFEQVSSEAGVAQSRTLRKILQQN GVEYLKQWL
Sbjct: 1   MEGEGREGGRGLMKIDELC-EEDVIDWFEQVSSEAGVAQSRTLRKILQQNYGVEYLKQWL 60

Query: 61  GDVNIHEIPDDFTLQSLFTSLIPLSSHSDFEPFLQRIAHGDSSPLLTQQPINTLSLSSGT 120
           GDVNIHEIPDDFTLQSLFTSL+PLSSH+DFEPFLQRIA GD SP LTQQPINTLSLSSGT
Sbjct: 61  GDVNIHEIPDDFTLQSLFTSLVPLSSHADFEPFLQRIADGDPSPCLTQQPINTLSLSSGT 120

Query: 121 TEGRQKYVPFTRHSAQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTG 180
           TEGRQKYVPFT HSAQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQ KTKGGITTG
Sbjct: 121 TEGRQKYVPFTSHSAQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQRKTKGGITTG 180

Query: 181 TATTHYYASEEFKIKQVKTKSFTCSPQEVISGCDYKQSTYCHLLLGLFYSEEVEFVTSTF 240
           TATTHYYASEEFKIKQVKTKSFTCSPQEVISGCDYKQSTYCHLLLGL YSEEVEFVTSTF
Sbjct: 181 TATTHYYASEEFKIKQVKTKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTF 240

Query: 241 AYTIVQALNQLEESWEEMCEDITNATLSSRIDIPKMRKGVLKVMSGNPGLGWKIGRVCEE 300
           AYTIVQALNQLEESWEE+C DIT++TLSSRI IPK+RK VLK+MS  PGLG KI RVCEE
Sbjct: 241 AYTIVQALNQLEESWEEICYDITHSTLSSRIAIPKIRKAVLKLMSPKPGLGRKIRRVCEE 300

Query: 301 LKREDWLGLIPKLWPNCKYVYSIMTGSMQPYLKKLRHYAGGLPLVSGDYGSTESWIGVNV 360
           LK EDWLGLIPKLWPNCKYVYSIMTGSMQPYLKKLRHY GGLPLVSGDYGSTESWIGVNV
Sbjct: 301 LKEEDWLGLIPKLWPNCKYVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNV 360

Query: 361 DPYLPPENVTFAVIPTFSYFEFIPLFKQQQQHYDSTSSDNTAIHDFLEGQPLPLSQVKMG 420
           DP LP ENVTFAVIPTFSYFEFIPLFKQQQ +Y STSSD TAIHDFLEGQP+PLSQVK+G
Sbjct: 361 DPCLPAENVTFAVIPTFSYFEFIPLFKQQQHYYSSTSSD-TAIHDFLEGQPIPLSQVKVG 420

Query: 421 QQYELVLTTFTGLYRYRLGDVVEVAGFHNKTPKLNFICRRKLVLSVNIDKNTEKDVQLVV 480
           QQYELVLTTFTGLYRYRLGDVVEVAGFHN TPKLNFICRRKLVL+VNIDKNTEKDVQLVV
Sbjct: 421 QQYELVLTTFTGLYRYRLGDVVEVAGFHNNTPKLNFICRRKLVLNVNIDKNTEKDVQLVV 480

Query: 481 ERGSQLLSDQSGAELVDFTSHAEVSSEQPGHYVIYWEVKGDVNDTVLQNCCSEMDGAFVD 540
           ERGSQ+LSDQSGAELV+FTSHAEV S+QPGHYVIYWEVKGDVND+VL++CCS MD AF+D
Sbjct: 481 ERGSQILSDQSGAELVEFTSHAEV-SDQPGHYVIYWEVKGDVNDSVLEDCCSAMDAAFID 540

Query: 541 YGYVVSRKANSIGALELRIVERGSFNKILEHYIGNGAALSQFKTPRCTTNHLLLNILNLS 600
           YGYVVSRKANSIG LELRIVERGSFNKILEHYIGNGAALSQFKTPRCTTN  LLNILNLS
Sbjct: 541 YGYVVSRKANSIGPLELRIVERGSFNKILEHYIGNGAALSQFKTPRCTTNPYLLNILNLS 600

Query: 601 TVKSFFSTAYA 611
           T+KSFFSTAYA
Sbjct: 601 TLKSFFSTAYA 608

[Close]
BLAST of Lsi10G008740 vs. NCBI nr
Match: TYJ96622.1 (jasmonic acid-amido synthetase JAR1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1074.3 bits (2777), Expect = 4.7e-310
Identity = 536/600 (89.33%), Postives = 564/600 (94.00%), Query Frame = 0 

Query: 12  MKIEELCEEEE-VIDWFEQVSSEAGVAQSRTLRKILQQNCGVEYLKQWLGDVNIHEIPDD 71
           MKI+ELCEEEE +IDWFEQVSSEA VAQSRTLRKILQQN GVEYLK+W+GDVNIHEIPDD
Sbjct: 1   MKIDELCEEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDD 60

Query: 72  FTLQSLFTSLIPLSSHSDFEPFLQRIAHGDSSPLLTQQPINTLSLSSGTTEGRQKYVPFT 131
           FTLQS+FTSLIPLSSH+ FEPFLQRIA GDSSPLLTQQPI TLSLSSGTTEGRQKYVPFT
Sbjct: 61  FTLQSIFTSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFT 120

Query: 132 RHSAQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEE 191
            HSA TTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEE
Sbjct: 121 PHSAHTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEE 180

Query: 192 FKIKQVKTKSFTCSPQEVISGCDYKQSTYCHLLLGLFYSEEVEFVTSTFAYTIVQALNQL 251
           FKIKQ+KTKSFTCSPQEVISGCDYKQSTYCHLLLGL YSEEVEFVTSTFAYTIVQA NQL
Sbjct: 181 FKIKQLKTKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQAFNQL 240

Query: 252 EESWEEMCEDITNATLSSRIDIPKMRKGVLKVMSGNPGLGWKIGRVCEELKREDWLGLIP 311
           EESWEE+  DI++ATLSSRIDIP++RK VLKVMS  P LGWKI RVCEELK+E WLGLIP
Sbjct: 241 EESWEEIIHDISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKKEGWLGLIP 300

Query: 312 KLWPNCKYVYSIMTGSMQPYLKKLRHYAGGLPLVSGDYGSTESWIGVNVDPYLPPENVTF 371
           KLWPNCKYVYSIMTGSMQPYLKKLRHY GGLPLVSGDYGSTESWIGVNVDPYLPPENVTF
Sbjct: 301 KLWPNCKYVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPPENVTF 360

Query: 372 AVIPTFSYFEFIPLFKQQQQHYDSTSSDNTAIHDFLEGQPLPLSQVKMGQQYELVLTTFT 431
           AVIPTFSYFEFIPLFKQ    + +TSS +TAIHDFLEGQP+ LS+VK+GQQYELVLTTFT
Sbjct: 361 AVIPTFSYFEFIPLFKQ----HATTSSTDTAIHDFLEGQPIALSEVKIGQQYELVLTTFT 420

Query: 432 GLYRYRLGDVVEVAGFHNKTPKLNFICRRKLVLSVNIDKNTEKDVQLVVERGSQLLSDQS 491
           GLYR RLGDVVEVAGFHNKTPKLNFICRRKLVL+VNIDKNTEKDVQL VERGSQL+S  S
Sbjct: 421 GLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQLMSQLS 480

Query: 492 GAELVDFTSHAEVSSEQPGHYVIYWEVKGDVNDTVLQNCCSEMDGAFVDYGYVVSRKANS 551
           GAELVDFTS+AE+S+ QPGHYVI+WEVKGDVNDTVL  CCSEMD AFVDYGYVVSRKANS
Sbjct: 481 GAELVDFTSYAELSN-QPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVVSRKANS 540

Query: 552 IGALELRIVERGSFNKILEHYIGNGAALSQFKTPRCTTNHLLLNILNLSTVKSFFSTAYA 611
           IG LELRIVERGSFNKILE+YIGNGAALSQFKTPRCTTNH LLNILNLST++SFFSTAYA
Sbjct: 541 IGPLELRIVERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSFFSTAYA 595

[Close]
BLAST of Lsi10G008740 vs. NCBI nr
Match: XP_008448228.1 (PREDICTED: jasmonic acid-amido synthetase JAR1 isoform X1 [Cucumis melo])

HSP 1 Score: 1074.3 bits (2777), Expect = 4.7e-310
Identity = 536/600 (89.33%), Postives = 564/600 (94.00%), Query Frame = 0 

Query: 12  MKIEELCEEEE-VIDWFEQVSSEAGVAQSRTLRKILQQNCGVEYLKQWLGDVNIHEIPDD 71
           MKI+ELCEEEE +IDWFEQVSSEA VAQSRTLRKILQQN GVEYLK+W+GDVNIHEIPDD
Sbjct: 1   MKIDELCEEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDD 60

Query: 72  FTLQSLFTSLIPLSSHSDFEPFLQRIAHGDSSPLLTQQPINTLSLSSGTTEGRQKYVPFT 131
           FTLQS+FTSLIPLSSH+ FEPFLQRIA GDSSPLLTQQPI TLSLSSGTTEGRQKYVPFT
Sbjct: 61  FTLQSIFTSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFT 120

Query: 132 RHSAQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEE 191
            HSA TTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEE
Sbjct: 121 PHSAHTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEE 180

Query: 192 FKIKQVKTKSFTCSPQEVISGCDYKQSTYCHLLLGLFYSEEVEFVTSTFAYTIVQALNQL 251
           FKIKQ+KTKSFTCSPQEVISGCDYKQSTYCHLLLGL YSEEVEFVTSTFAYTIVQA NQL
Sbjct: 181 FKIKQLKTKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQAFNQL 240

Query: 252 EESWEEMCEDITNATLSSRIDIPKMRKGVLKVMSGNPGLGWKIGRVCEELKREDWLGLIP 311
           EESWEE+  DI++ATLSSRIDIP++RK VLKVMS  P LGWKI RVCEELK+E WLGLIP
Sbjct: 241 EESWEEIIHDISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKKEGWLGLIP 300

Query: 312 KLWPNCKYVYSIMTGSMQPYLKKLRHYAGGLPLVSGDYGSTESWIGVNVDPYLPPENVTF 371
           KLWPNCKYVYSIMTGSMQPYLKKLRHY GGLPLVSGDYGSTESWIGVNVDPYLPPENVTF
Sbjct: 301 KLWPNCKYVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPPENVTF 360

Query: 372 AVIPTFSYFEFIPLFKQQQQHYDSTSSDNTAIHDFLEGQPLPLSQVKMGQQYELVLTTFT 431
           AVIPTFSYFEFIPLFKQ    + +TSS +TAIHDFLEGQP+ LS+VK+GQQYELVLTTFT
Sbjct: 361 AVIPTFSYFEFIPLFKQ----HATTSSTDTAIHDFLEGQPIALSEVKIGQQYELVLTTFT 420

Query: 432 GLYRYRLGDVVEVAGFHNKTPKLNFICRRKLVLSVNIDKNTEKDVQLVVERGSQLLSDQS 491
           GLYR RLGDVVEVAGFHNKTPKLNFICRRKLVL+VNIDKNTEKDVQL VERGSQL+S  S
Sbjct: 421 GLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQLMSQLS 480

Query: 492 GAELVDFTSHAEVSSEQPGHYVIYWEVKGDVNDTVLQNCCSEMDGAFVDYGYVVSRKANS 551
           GAELVDFTS+AE+S+ QPGHYVI+WEVKGDVNDTVL  CCSEMD AFVDYGYVVSRKANS
Sbjct: 481 GAELVDFTSYAELSN-QPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVVSRKANS 540

Query: 552 IGALELRIVERGSFNKILEHYIGNGAALSQFKTPRCTTNHLLLNILNLSTVKSFFSTAYA 611
           IG LELRIVERGSFNKILE+YIGNGAALSQFKTPRCTTNH LLNILNLST++SFFSTAYA
Sbjct: 541 IGPLELRIVERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSFFSTAYA 595

[Close]
BLAST of Lsi10G008740 vs. NCBI nr
Match: XP_004140048.1 (indole-3-acetic acid-amido synthetase GH3.10 isoform X1 [Cucumis sativus] >KGN46697.1 hypothetical protein Csa_021068 [Cucumis sativus])

HSP 1 Score: 1050.0 bits (2714), Expect = 7.6e-303
Identity = 521/591 (88.16%), Postives = 551/591 (93.23%), Query Frame = 0 

Query: 20  EEEVIDWFEQVSSEAGVAQSRTLRKILQQNCGVEYLKQWLGDVNIHEIPDDFTLQSLFTS 79
           EE++I WFEQVSSEA +AQSRTLRKILQQN GVEYLK+W+GDVNIHEIPDDFTL S+FTS
Sbjct: 4   EEDIIGWFEQVSSEAALAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDDFTLHSIFTS 63

Query: 80  LIPLSSHSDFEPFLQRIAHGDSSPLLTQQPINTLSLSSGTTEGRQKYVPFTRHSAQTTLL 139
            IPLSSH+ FEPFLQRIA GDSSPLLTQQPI TLSLSSGTTEGRQKYVPFT HSAQTTLL
Sbjct: 64  SIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHSAQTTLL 123

Query: 140 IFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQVKTK 199
           IFRLAAAYRSRVYP R+GGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQ+KTK
Sbjct: 124 IFRLAAAYRSRVYPIRDGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLKTK 183

Query: 200 SFTCSPQEVISGCDYKQSTYCHLLLGLFYSEEVEFVTSTFAYTIVQALNQLEESWEEMCE 259
           SFTCSPQEVI+GCDYKQSTYCHLLLGL YSEEVEFVTSTFAYTIVQA NQLEESWEEM  
Sbjct: 184 SFTCSPQEVITGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQAFNQLEESWEEMIH 243

Query: 260 DITNATLSSRIDIPKMRKGVLKVMSGNPGLGWKIGRVCEELKREDWLGLIPKLWPNCKYV 319
           D+++ATLSSRIDIP++RK VLKVMS  P LGWKI RVCEEL +E WLGLIPKLWPNCKYV
Sbjct: 244 DLSHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELMKEGWLGLIPKLWPNCKYV 303

Query: 320 YSIMTGSMQPYLKKLRHYAGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFSYF 379
           YSIMTGSMQPYLKKLRHY GGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFSYF
Sbjct: 304 YSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFSYF 363

Query: 380 EFIPLFKQQQQHYDSTSSDNTAIHDFLEGQPLPLSQVKMGQQYELVLTTFTGLYRYRLGD 439
           EFIPLFKQ    + +TSS +TAIHDFLEGQP+ LS+VK+GQQYELVLTTFTGLYR RLGD
Sbjct: 364 EFIPLFKQ----HATTSSADTAIHDFLEGQPIALSEVKIGQQYELVLTTFTGLYRCRLGD 423

Query: 440 VVEVAGFHNKTPKLNFICRRKLVLSVNIDKNTEKDVQLVVERGSQLLSDQSGAELVDFTS 499
           VVEVAGFHNKTPKLNFICRRKLVL+VNIDKNTEKDVQL VERGSQL+S  SGAELVDFTS
Sbjct: 424 VVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQLMSQLSGAELVDFTS 483

Query: 500 HAEVSSEQPGHYVIYWEVKGDVNDTVLQNCCSEMDGAFVDYGYVVSRKANSIGALELRIV 559
           +AE+S+ QPGHYVI+WE+KGDVND VL  CCSEMD AFVDYGYVVSRKANSIG LELRIV
Sbjct: 484 YAELSN-QPGHYVIFWELKGDVNDKVLGMCCSEMDAAFVDYGYVVSRKANSIGPLELRIV 543

Query: 560 ERGSFNKILEHYIGNGAALSQFKTPRCTTNHLLLNILNLSTVKSFFSTAYA 611
           ERGSFNKILEHYIGNGAALSQFKTPRCTTNH LLNILNLST+KSFFSTAYA
Sbjct: 544 ERGSFNKILEHYIGNGAALSQFKTPRCTTNHFLLNILNLSTLKSFFSTAYA 589

[Close]
BLAST of Lsi10G008740 vs. NCBI nr
Match: KAA0065353.1 (jasmonic acid-amido synthetase JAR1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1044.6 bits (2700), Expect = 3.2e-301
Identity = 527/605 (87.11%), Postives = 557/605 (92.07%), Query Frame = 0 

Query: 12  MKIEELCEEEE-VIDWFEQVSSEAGVAQSRTLRKILQQNCGVEYLKQWLGDVNIHEIPDD 71
           MKI+ELCEEEE +IDWFEQVSSEA VAQSRTLRKILQQN GVEYLK+W+GDVNIHEIPDD
Sbjct: 1   MKIDELCEEEEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDD 60

Query: 72  FTLQSLFTSLIPLSSHSDFEPFLQRIAHGDSSPLLTQQPINTLSLSSGTTEGRQKYVPFT 131
           FTLQS+FTSLIPLSSH+ FEPFLQRIA GDSSPLLTQQPI TLSLSSGTTEGRQKYVPFT
Sbjct: 61  FTLQSIFTSLIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFT 120

Query: 132 RHSAQTTLLIFRLAAAYRSRVYPTREG-----GKILEFIYSSKQTKTKGGITTGTATTHY 191
            HSAQTTLLIFRLAAAYRS     ++        ILEFIYSSKQTKTKGGITTGTATTHY
Sbjct: 121 PHSAQTTLLIFRLAAAYRSTNITQKQHLLTIYLLILEFIYSSKQTKTKGGITTGTATTHY 180

Query: 192 YASEEFKIKQVKTKSFTCSPQEVISGCDYKQSTYCHLLLGLFYSEEVEFVTSTFAYTIVQ 251
           YASEEFKIKQ+KTKSFTCSPQEVISGCDYKQSTYCHLLLGL YSEEVEFVTSTFAYTIVQ
Sbjct: 181 YASEEFKIKQLKTKSFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQ 240

Query: 252 ALNQLEESWEEMCEDITNATLSSRIDIPKMRKGVLKVMSGNPGLGWKIGRVCEELKREDW 311
           A NQLEESWEE+  DI++ATLSSRIDIP++RK VLKVMS  P LGWKI RVCEELK+E W
Sbjct: 241 AFNQLEESWEEIIHDISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKKEGW 300

Query: 312 LGLIPKLWPNCKYVYSIMTGSMQPYLKKLRHYAGGLPLVSGDYGSTESWIGVNVDPYLPP 371
           LGLIPKLWPNCKYVYSIMTGSMQPYLKKLRHY GGLPLVSGDYGSTESWIGVNVDPYLPP
Sbjct: 301 LGLIPKLWPNCKYVYSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPP 360

Query: 372 ENVTFAVIPTFSYFEFIPLFKQQQQHYDSTSSDNTAIHDFLEGQPLPLSQVKMGQQYELV 431
           ENVTFAVIPTFSYFEFIPLFKQ    + +TSS +TAIHDFLEGQP+ LS+VK+GQQYELV
Sbjct: 361 ENVTFAVIPTFSYFEFIPLFKQ----HATTSSTDTAIHDFLEGQPIALSEVKIGQQYELV 420

Query: 432 LTTFTGLYRYRLGDVVEVAGFHNKTPKLNFICRRKLVLSVNIDKNTEKDVQLVVERGSQL 491
           LTTFTGLYR RLGDVVEVAGFHNKTPKLNFICRRKLVL+VNIDKNTEKDVQL VERGSQL
Sbjct: 421 LTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQL 480

Query: 492 LSDQSGAELVDFTSHAEVSSEQPGHYVIYWEVKGDVNDTVLQNCCSEMDGAFVDYGYVVS 551
           +S  SGAELVDFTS+AE+S+ QPGHYVI+WEVKGDVNDTVL  CCSEMD AFVDYGYVVS
Sbjct: 481 MSQLSGAELVDFTSYAELSN-QPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVVS 540

Query: 552 RKANSIGALELRIVERGSFNKILEHYIGNGAALSQFKTPRCTTNHLLLNILNLSTVKSFF 611
           RKANSIG LELRIVERGSFNKILE+YIGNGAALSQFKTPRCTTNH LLNILNLST++SFF
Sbjct: 541 RKANSIGPLELRIVERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSFF 600

[Close]
BLAST of Lsi10G008740 vs. TAIR 10
Match: AT4G03400.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 812.0 bits (2096), Expect = 3.3e-235
Identity = 399/590 (67.63%), Postives = 477/590 (80.85%), Query Frame = 0 

Query: 21  EEVIDWFEQVSSEAGVAQSRTLRKILQQNCGVEYLKQWLGDVNIHEIPDDFTLQSLFTSL 80
           ++VI WFE VS  A   QS TLR+IL+ N GVEYL++WLG V++ ++ DD+TL++LFTSL
Sbjct: 9   DDVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKM-DDYTLETLFTSL 68

Query: 81  IPLSSHSDFEPFLQRIAHGDSSPLLTQQPINTLSLSSGTTEGRQKYVPFTRHSAQTTLLI 140
           +P+ SH+D +P++QRIA G++SPLLTQ+PI  LSLSSGTTEGRQKYVPFTRHSAQTTL I
Sbjct: 69  VPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQTTLQI 128

Query: 141 FRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQVKTKS 200
           FRL+AAYRSR YP REGG+ILEFIY+ K+ KT GG+T GTATTHYYASEEFK KQ  TKS
Sbjct: 129 FRLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYYASEEFKTKQETTKS 188

Query: 201 FTCSPQEVISGCDYKQSTYCHLLLGLFYSEEVEFVTSTFAYTIVQALNQLEESWEEMCED 260
           FTCSPQEVISG D+ Q TYCHLLLGL YS +VEFV S F+YTIVQA +  EE W E+C D
Sbjct: 189 FTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQAFSFFEEIWREICAD 248

Query: 261 ITNATLSSRIDIPKMRKGVLKVMSGNPGLGWKIGRVCEELKRE-DWLGLIPKLWPNCKYV 320
           I    LSSRI +PKMRK VL ++  NP L   I  +C EL+    W GLI KLWPN K++
Sbjct: 249 IKEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLGWFGLISKLWPNAKFI 308

Query: 321 YSIMTGSMQPYLKKLRHYAGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFSYF 380
            SIMTGSM PYL KLRHYAGGLPLVS DYGSTESWIGVNVDP+LPPE+V+FAVIPTFSYF
Sbjct: 309 SSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLPPEDVSFAVIPTFSYF 368

Query: 381 EFIPLFKQQQQHYDSTSSDNTAIHDFLEGQPLPLSQVKMGQQYELVLTTFTGLYRYRLGD 440
           EFIPL+++Q Q      SD     DF+E +P+PLSQVK+GQ+YELVLTTFTGLYRYRLGD
Sbjct: 369 EFIPLYRRQNQ------SDICIDGDFVEDKPVPLSQVKLGQEYELVLTTFTGLYRYRLGD 428

Query: 441 VVEVAGFHNKTPKLNFICRRKLVLSVNIDKNTEKDVQLVVERGSQLLSDQSGAELVDFTS 500
           VVEV  FH  TPKL+FI RRKL+L++NIDKNTEKD+Q VV++ SQLLS  + AE+VDFTS
Sbjct: 429 VVEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQLLSRSTRAEVVDFTS 488

Query: 501 HAEVSSEQPGHYVIYWEVKGDVNDTVLQNCCSEMDGAFVDYGYVVSRKANSIGALELRIV 560
           HA+V + +PGHYVIYWE++G+ +D  L+ CC EMD AFVDYGYVVSR+ NSIG LELR+V
Sbjct: 489 HADVIA-RPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSRRMNSIGPLELRVV 548

Query: 561 ERGSFNKILEHYIGNGAALSQFKTPRCTTNHLLLNILNLSTVKSFFSTAY 610
           ERG+F K+ E  +G    L+QFKTPRCTTN ++L+ILN ST+K F S+AY
Sbjct: 549 ERGTFGKVAERCVGKCGGLNQFKTPRCTTNSVMLDILNDSTIKRFRSSAY 590

[Close]
BLAST of Lsi10G008740 vs. TAIR 10
Match: AT2G46370.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 549.7 bits (1415), Expect = 3.0e-156
Identity = 279/596 (46.81%), Postives = 399/596 (66.95%), Query Frame = 0 

Query: 16  ELCEEEEVIDWFEQVSSEAGVAQSRTLRKILQQNCGVEYLKQWLGDVNIHEIPDDFTLQS 75
           E  +   VID F++++  A   Q +TL++IL +N    YL+    + N  + P++     
Sbjct: 6   ETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATD-PEE----- 65

Query: 76  LFTSLIPLSSHSDFEPFLQRIAHGDSSPLLTQQPINTLSLSSGTTEGRQKYVPFTRHSAQ 135
            F S++PL +  + EP+++R+  GD+SP+LT  P+  +SLSSGT++GR K++PFT    +
Sbjct: 66  AFKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELME 125

Query: 136 TTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQ 195
            TL +FR A A+R+R +P  + GK L+FI+SSKQ  + GG+  GTATT+ Y +  FK   
Sbjct: 126 NTLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGM 185

Query: 196 VKTKSFTCSPQEVISGCDYKQSTYCHLLLGLFYSEEVEFVTSTFAYTIVQALNQLEESWE 255
               S +CSP EVI   D  Q+ YCHLL G+ + ++V++V + FA+ +V A    E+ WE
Sbjct: 186 KSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWE 245

Query: 256 EMCEDITNATLSSRIDIPKMRKGVLKVMSGNPGLGWKIGRVCEELKREDWLGLIPKLWPN 315
           E+  DI +  LS+RI +P +R  + K+++ NP L   I   C  L   +W GLIP L+PN
Sbjct: 246 EIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPN 305

Query: 316 CKYVYSIMTGSMQPYLKKLRHYAGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPT 375
            KYVY IMTGSM+PY+ KLRHYAG LPLVS DYGS+E WI  NV P L PE  TFAVIP 
Sbjct: 306 AKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPN 365

Query: 376 FSYFEFIPLFKQQQQHYDSTSSDNTAIHDFLEGQPLPLSQVKMGQQYELVLTTFTGLYRY 435
             YFEF+P+ +  +                 E +P+ L+QVK+G++YE+V+T + GLYRY
Sbjct: 366 LGYFEFLPVSETGEG----------------EEKPVGLTQVKIGEEYEVVITNYAGLYRY 425

Query: 436 RLGDVVEVAGFHNKTPKLNFICRRKLVLSVNIDKNTEKDVQLVVERGSQLLSDQSGAELV 495
           RLGDVV+V GF+N TP+L FICRR L+LS+NIDKNTE+D+QL VE  ++ LS++   E++
Sbjct: 426 RLGDVVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK-IEVI 485

Query: 496 DFTSHAEVSSEQPGHYVIYWEVKGDVNDTVLQNCCSEMDGAFVDYGYVVSRKANSIGALE 555
           DF+S+ +VS++ PGHY I+WE+ G+ N+ VLQ+CC+ +D AF+D GYV SRK  +IGALE
Sbjct: 486 DFSSYIDVSTD-PGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALE 545

Query: 556 LRIVERGSFNKILEHYIGNGAALSQFKTPRCT--TNHLLLNILNLSTVKSFFSTAY 610
           LR+V +G+F KI EH++G G++  QFK PRC   +N  +L IL  + V S+FSTA+
Sbjct: 546 LRVVAKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

[Close]
BLAST of Lsi10G008740 vs. TAIR 10
Match: AT2G46370.2 (Auxin-responsive GH3 family protein )

HSP 1 Score: 549.7 bits (1415), Expect = 3.0e-156
Identity = 279/596 (46.81%), Postives = 399/596 (66.95%), Query Frame = 0 

Query: 16  ELCEEEEVIDWFEQVSSEAGVAQSRTLRKILQQNCGVEYLKQWLGDVNIHEIPDDFTLQS 75
           E  +   VID F++++  A   Q +TL++IL +N    YL+    + N  + P++     
Sbjct: 6   ETFDMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATD-PEE----- 65

Query: 76  LFTSLIPLSSHSDFEPFLQRIAHGDSSPLLTQQPINTLSLSSGTTEGRQKYVPFTRHSAQ 135
            F S++PL +  + EP+++R+  GD+SP+LT  P+  +SLSSGT++GR K++PFT    +
Sbjct: 66  AFKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELME 125

Query: 136 TTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQ 195
            TL +FR A A+R+R +P  + GK L+FI+SSKQ  + GG+  GTATT+ Y +  FK   
Sbjct: 126 NTLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGM 185

Query: 196 VKTKSFTCSPQEVISGCDYKQSTYCHLLLGLFYSEEVEFVTSTFAYTIVQALNQLEESWE 255
               S +CSP EVI   D  Q+ YCHLL G+ + ++V++V + FA+ +V A    E+ WE
Sbjct: 186 KSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWE 245

Query: 256 EMCEDITNATLSSRIDIPKMRKGVLKVMSGNPGLGWKIGRVCEELKREDWLGLIPKLWPN 315
           E+  DI +  LS+RI +P +R  + K+++ NP L   I   C  L   +W GLIP L+PN
Sbjct: 246 EIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPN 305

Query: 316 CKYVYSIMTGSMQPYLKKLRHYAGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPT 375
            KYVY IMTGSM+PY+ KLRHYAG LPLVS DYGS+E WI  NV P L PE  TFAVIP 
Sbjct: 306 AKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPN 365

Query: 376 FSYFEFIPLFKQQQQHYDSTSSDNTAIHDFLEGQPLPLSQVKMGQQYELVLTTFTGLYRY 435
             YFEF+P+ +  +                 E +P+ L+QVK+G++YE+V+T + GLYRY
Sbjct: 366 LGYFEFLPVSETGEG----------------EEKPVGLTQVKIGEEYEVVITNYAGLYRY 425

Query: 436 RLGDVVEVAGFHNKTPKLNFICRRKLVLSVNIDKNTEKDVQLVVERGSQLLSDQSGAELV 495
           RLGDVV+V GF+N TP+L FICRR L+LS+NIDKNTE+D+QL VE  ++ LS++   E++
Sbjct: 426 RLGDVVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK-IEVI 485

Query: 496 DFTSHAEVSSEQPGHYVIYWEVKGDVNDTVLQNCCSEMDGAFVDYGYVVSRKANSIGALE 555
           DF+S+ +VS++ PGHY I+WE+ G+ N+ VLQ+CC+ +D AF+D GYV SRK  +IGALE
Sbjct: 486 DFSSYIDVSTD-PGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALE 545

Query: 556 LRIVERGSFNKILEHYIGNGAALSQFKTPRCT--TNHLLLNILNLSTVKSFFSTAY 610
           LR+V +G+F KI EH++G G++  QFK PRC   +N  +L IL  + V S+FSTA+
Sbjct: 546 LRVVAKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

[Close]
BLAST of Lsi10G008740 vs. TAIR 10
Match: AT2G46370.4 (Auxin-responsive GH3 family protein )

HSP 1 Score: 549.7 bits (1415), Expect = 3.0e-156
Identity = 282/603 (46.77%), Postives = 403/603 (66.83%), Query Frame = 0 

Query: 9   KELMKIEELCEEEEVIDWFEQVSSEAGVAQSRTLRKILQQNCGVEYLKQWLGDVNIHEIP 68
           K L K+E   +   VID F++++  A   Q +TL++IL +N    YL+    + N  + P
Sbjct: 11  KMLEKVETF-DMNRVIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGNATD-P 70

Query: 69  DDFTLQSLFTSLIPLSSHSDFEPFLQRIAHGDSSPLLTQQPINTLSLSSGTTEGRQKYVP 128
           ++      F S++PL +  + EP+++R+  GD+SP+LT  P+  +SLSSGT++GR K++P
Sbjct: 71  EE-----AFKSMVPLVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIP 130

Query: 129 FTRHSAQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYAS 188
           FT    + TL +FR A A+R+R +P  + GK L+FI+SSKQ  + GG+  GTATT+ Y +
Sbjct: 131 FTDELMENTLQLFRTAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRN 190

Query: 189 EEFKIKQVKTKSFTCSPQEVISGCDYKQSTYCHLLLGLFYSEEVEFVTSTFAYTIVQALN 248
             FK       S +CSP EVI   D  Q+ YCHLL G+ + ++V++V + FA+ +V A  
Sbjct: 191 PNFKAGMKSITSPSCSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFR 250

Query: 249 QLEESWEEMCEDITNATLSSRIDIPKMRKGVLKVMSGNPGLGWKIGRVCEELKREDWLGL 308
             E+ WEE+  DI +  LS+RI +P +R  + K+++ NP L   I   C  L   +W GL
Sbjct: 251 TFEQVWEEIVTDIKDGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGL 310

Query: 309 IPKLWPNCKYVYSIMTGSMQPYLKKLRHYAGGLPLVSGDYGSTESWIGVNVDPYLPPENV 368
           IP L+PN KYVY IMTGSM+PY+ KLRHYAG LPLVS DYGS+E WI  NV P L PE  
Sbjct: 311 IPALFPNAKYVYGIMTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEA 370

Query: 369 TFAVIPTFSYFEFIPLFKQQQQHYDSTSSDNTAIHDFLEGQPLPLSQVKMGQQYELVLTT 428
           TFAVIP   YFEF+P+ +  +                 E +P+ L+QVK+G++YE+V+T 
Sbjct: 371 TFAVIPNLGYFEFLPVSETGEG----------------EEKPVGLTQVKIGEEYEVVITN 430

Query: 429 FTGLYRYRLGDVVEVAGFHNKTPKLNFICRRKLVLSVNIDKNTEKDVQLVVERGSQLLSD 488
           + GLYRYRLGDVV+V GF+N TP+L FICRR L+LS+NIDKNTE+D+QL VE  ++ LS+
Sbjct: 431 YAGLYRYRLGDVVKVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSE 490

Query: 489 QSGAELVDFTSHAEVSSEQPGHYVIYWEVKGDVNDTVLQNCCSEMDGAFVDYGYVVSRKA 548
           +   E++DF+S+ +VS++ PGHY I+WE+ G+ N+ VLQ+CC+ +D AF+D GYV SRK 
Sbjct: 491 EK-IEVIDFSSYIDVSTD-PGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKC 550

Query: 549 NSIGALELRIVERGSFNKILEHYIGNGAALSQFKTPRCT--TNHLLLNILNLSTVKSFFS 608
            +IGALELR+V +G+F KI EH++G G++  QFK PRC   +N  +L IL  + V S+FS
Sbjct: 551 KTIGALELRVVAKGTFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFS 586

Query: 609 TAY 610
           TA+
Sbjct: 611 TAF 586

[Close]
BLAST of Lsi10G008740 vs. TAIR 10
Match: AT2G46370.3 (Auxin-responsive GH3 family protein )

HSP 1 Score: 518.1 bits (1333), Expect = 9.7e-147
Identity = 256/513 (49.90%), Postives = 354/513 (69.01%), Query Frame = 0 

Query: 99  GDSSPLLTQQPINTLSLSSGTTEGRQKYVPFTRHSAQTTLLIFRLAAAYRSRVYPTREGG 158
           GD+SP+LT  P+  +SLSSGT++GR K++PFT    + TL +FR A A+R+R +P  + G
Sbjct: 4   GDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLFRTAFAFRNRDFPIDDNG 63

Query: 159 KILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQVKTKSFTCSPQEVISGCDYKQST 218
           K L+FI+SSKQ  + GG+  GTATT+ Y +  FK       S +CSP EVI   D  Q+ 
Sbjct: 64  KALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSPSCSPDEVIFSPDVHQAL 123

Query: 219 YCHLLLGLFYSEEVEFVTSTFAYTIVQALNQLEESWEEMCEDITNATLSSRIDIPKMRKG 278
           YCHLL G+ + ++V++V + FA+ +V A    E+ WEE+  DI +  LS+RI +P +R  
Sbjct: 124 YCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDIKDGVLSNRITVPSVRTA 183

Query: 279 VLKVMSGNPGLGWKIGRVCEELKREDWLGLIPKLWPNCKYVYSIMTGSMQPYLKKLRHYA 338
           + K+++ NP L   I   C  L   +W GLIP L+PN KYVY IMTGSM+PY+ KLRHYA
Sbjct: 184 MSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPNAKYVYGIMTGSMEPYVPKLRHYA 243

Query: 339 GGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFSYFEFIPLFKQQQQHYDSTSSD 398
           G LPLVS DYGS+E WI  NV P L PE  TFAVIP   YFEF+P+ +  +         
Sbjct: 244 GDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEFLPVSETGEG-------- 303

Query: 399 NTAIHDFLEGQPLPLSQVKMGQQYELVLTTFTGLYRYRLGDVVEVAGFHNKTPKLNFICR 458
                   E +P+ L+QVK+G++YE+V+T + GLYRYRLGDVV+V GF+N TP+L FICR
Sbjct: 304 --------EEKPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNTPQLKFICR 363

Query: 459 RKLVLSVNIDKNTEKDVQLVVERGSQLLSDQSGAELVDFTSHAEVSSEQPGHYVIYWEVK 518
           R L+LS+NIDKNTE+D+QL VE  ++ LS++   E++DF+S+ +VS++ PGHY I+WE+ 
Sbjct: 364 RNLILSINIDKNTERDLQLSVESAAKRLSEEK-IEVIDFSSYIDVSTD-PGHYAIFWEIS 423

Query: 519 GDVNDTVLQNCCSEMDGAFVDYGYVVSRKANSIGALELRIVERGSFNKILEHYIGNGAAL 578
           G+ N+ VLQ+CC+ +D AF+D GYV SRK  +IGALELR+V +G+F KI EH++G G++ 
Sbjct: 424 GETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFLGLGSSA 483

Query: 579 SQFKTPRCT--TNHLLLNILNLSTVKSFFSTAY 610
            QFK PRC   +N  +L IL  + V S+FSTA+
Sbjct: 484 GQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 496

The following BLAST results are available for this feature:
BLAST of Lsi10G008740 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9ZNS24.6e-23467.63Indole-3-acetic acid-amido synthetase GH3.10 OS=Arabidopsis thaliana OX=3702 GN=... [more]
A0A1J6KGJ93.7e-17551.41Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 ... [more]
A0A314KSQ41.1e-17151.42Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 ... [more]
Q6I5811.4e-16650.50Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=3994... [more]
Q9SKE24.3e-15546.81Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 ... [more]
BLAST of Lsi10G008740 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3B9T42.3e-31089.33Jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3BIL62.3e-31089.33jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0KE573.7e-30388.16Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G124120 PE=3 SV=1[more]
A0A5A7VB011.6e-30187.11Jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo var. makuwa OX=11... [more]
A0A6J1FRQ17.0e-29484.72indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita moschata OX=3662 GN=LO... [more]
BLAST of Lsi10G008740 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038900326.10.0e+0091.00indole-3-acetic acid-amido synthetase GH3.10 [Benincasa hispida][more]
TYJ96622.14.7e-31089.33jasmonic acid-amido synthetase JAR1 isoform X1 [Cucumis melo var. makuwa][more]
XP_008448228.14.7e-31089.33PREDICTED: jasmonic acid-amido synthetase JAR1 isoform X1 [Cucumis melo][more]
XP_004140048.17.6e-30388.16indole-3-acetic acid-amido synthetase GH3.10 isoform X1 [Cucumis sativus] >KGN46... [more]
KAA0065353.13.2e-30187.11jasmonic acid-amido synthetase JAR1 isoform X1 [Cucumis melo var. makuwa][more]
BLAST of Lsi10G008740 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G03400.13.3e-23567.63Auxin-responsive GH3 family protein [more]
AT2G46370.13.0e-15646.81Auxin-responsive GH3 family protein [more]
AT2G46370.23.0e-15646.81Auxin-responsive GH3 family protein [more]
AT2G46370.43.0e-15646.77Auxin-responsive GH3 family protein [more]
AT2G46370.39.7e-14749.90Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 26..589
e-value: 1.7E-176
score: 588.2
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 18..609
NoneNo IPR availablePANTHERPTHR31901:SF48INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.10coord: 18..609

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G008740.1Lsi10G008740.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009416 response to light stimulus
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016881 acid-amino acid ligase activity