Lsi10G008610 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G008610
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionvesicle-associated protein 4-2-like
Locationchr10: 12786056 .. 12790230 (+)
RNA-Seq ExpressionLsi10G008610
SyntenyLsi10G008610
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGTCGCCAATGAGAAATCAGGTCACGATGAGAAGGTTTGGACCCTTTGTAGTATTCCCTTTTGGCAAAACAGCAATGCTCCGTCTTCTGCTTCTTCATCGTCTCTTATTCATAGAGTTCATTTGCAAAGCCACAGTCATAAGCCAGCAGAACATTCGAGTTCGCATCCTGGTGTGGTTTCGGCAATTGCCAAATCTCTACTTCCTACCAAAAGAAGACTTCGTCTCGATCCTTCCAACAAGCTCTACTTTCCATGTATGTCTCCGGTGGCTCTTTTCTCTTGTGCTTGGTTGATTTTCTGCCTCGTGGGGTACTTGGTTTTTGGTTTATTTATAGGGTTTAGTGTTTCCTTGATTAAGGTAGTTGATTGTTTCATCTGTGTCGTCATGTTTCTGGGTTCATAATGAGAATTTTGTTGCCTTTTCGTTCTGTTCTTTAACTGTGTTTGGTTTCTGAATGACAGAATGTCTTTTTTGCTTCTTTCCTCTTTATCAATGTGGAAGCTCTAATTTTTGGTTTCTTGCTTTTCTGATCCGGCAATGAACTGAGTTAAAAATGAATTTTAATGTGTTGATTTAGTTATCCTCCCCTGTTCCTTGGTGAAGCTTACAAAAGGATGACGATTGATGACTACTTAGTTACTCTCTAACAGATGTTGTCTGAGTTTAAAACTCATGCACCCAAAAAGGATATGTTCTTGTTTGGGTTTATAGAATGTGTTTTTTCTTTTCTTTTTGTTCATTTTTTCAGATCGCTTCCTCTCTATTTGGTTATATAGGATAGTTTGTTTCGTCGTCTAACAAGAATGGGGCCTTTGAATTGCATATATTACTTGTTGTATCATTTTAGTTGTTGACTAGATTTGTTTTTCCTCACAACTGTGTTTTGATTTCATTCTTTCATTTCTTCTTTCCCCTTAAAGTTGAACCTGGGAAGCAGGTCAAGAGTGCTATTGAGATAAAGAATACGAGCAAGAATCATGTGGCTTTCAAAGTATGGATTATTTCCCTCTCGAACTTTTATCAGTATGAATACTGAGTTGTTTAGTTTTATTATAGATGCCAAACTACCTTTGTTATATTAATCTACCCAATGCAGCCATCTTACTTTTGATCCCTTTAATTGTTGTAGTTCCAAACGACCGCACCGAAGAGCTGTTACATGCGTCCTCCTGGTGGTATACTTGCTCCTGATGAAAGTATTATTGCAACGGGTAAGACATTTGTCTGGCATTTGATCTGTATCCTTAGAATTCAACATTGCAAGTAGAAACAATGTAATTAAATCATTTGGAATTTTGCTGAGGTTCCCATATCTGTGACTTGACTTCAGTATTCAAGTTTGTGGAGCCACTAGATAACAATGACAAGCATGTAGATCATAAGAGAAGGGTCAAGTTCAAGATTGTGAGCTTGAAAGTGAAGGGCGACATGGACTACGTGCCTGAGCTGGTATGTCTTGCAATCTCAAATTCTGAAATAGTTACTGTTTTTCCATGCTTAGAGTTTGTAACCAATGCTTGTATCATTATTTCTCAAGATGAGTATTTACTATTTCAATGATCTATGAAGATTAATGTCTGCTGACATTTCAAGATAACTTCCTGAGATATAGTTTTGTTGCATTCACAATGACGAAAATGAATGTTAGTCTAATTAAGTACTTTTAGTTGCATATTTATCAATCCATGATGCAATCTTCTAGTTTTCTTATGTGAAAGCCTAATTATAATTCAGTCTAGTATTATAACTTTCTGATGGTAATTTCAAATAATGGCATTTTACAGTTCGATGAGCAAAAAGATCATGTAGCAGTTGAGCAAATATTGCAAGTTGTTTTTCTTGACCCGGATCATTCTAATCCTGTAAGTAGTAACAGGAGATAAACACAATATCAAGTACAGCCATGATTGCTTCTCGCATCAATCTTCTTATCGGTTCGGATTCTTCTCCTTTATTCTTTTTCAGGCACTAGAGAAAGTCTTTCGACAACTAGCTGAGGCTGAGGCTGCAATTGAGACACGCCAAAAGCCTGCTGAAGAAGCAGACCCGAAAATTGTTGGTGGAGGACTTGTCCTCGACGAATGGGTGAGATAACTAGACTGTTGTCCACATCTTCATCAAACCTTACTGAGTTTATTTGAACATTTACATTCTCTGACATACTTGATGCTATGGTTCTTCATTTGTAGAAAGAAAGAAGGGAAAGATATCTAGCTAAGCAACAGGTTGGAGGTGTGGGTTTGGTGTAGAATTCAGTTGCTTGTGTTATGGAAGTTGAAATCACATTCATGTGTGAACTTTTCATTGAGCCATTTGTCGAAGGAGCAACCATCGACGTTAGCAATCAAAGGTGAATGGCTTAATGACTAGATTTGTGATGGAGTTTCATGATCTTGTGTTTGTTTGATTTGGGTTGCTGTTTCTCAAAGTTGATTTGAAACACTGTCACCATTATTGATGTAAATAGTGGCTGTGCTGTTGCTTCGCCTCAACCTAAAGTTTGACACCAAATTTGGCAAGTCTAATCTACATTTTTATTTGAAAATGCCTTTTCCTATTTGATGGTTTTGCTGTTTGTGATAAGATTTAGGATAAATTTAGCCAAAGAATCAAGTCTTCAAATAGAATATATCCTTATTCTTCTTTGTGAATCTTACCCAGAAGCATCTCTAAATGAAACAATTGAGAGACATCATTTTAGCCCACTTGCTTTCTAGAATCTCTTTGTTCTGTCCCTTTCTTTCAAATCTTTTCCTCAGTGGTTTCATATCATATAAGGCACGAAAGGACTCAAAATTTGTTCTTTTTTTTTTTTTCTTTTTTCTTTTCTCCCTCTAAGTGGAGACTTAATGGATAAAAACAATTATAGCTTATTCCCCCATCATGTTATGAATATATTCCAATGAACCCCACCTTCAACAATTGCATTATTTATCTCTAATTTCAAACTTATTAATTTAAATCAAGTGAATAATGCTAAAATGTTTATGTGTGGATAAATAGATGGTGAATTACATATCCATTACTATTAAACATGGGGTTGAAAGACAAAATTGGAAATTTAAACTTATTTATGTGTAGATGTACATTTAAAACTAACTGATAAAAGCTTTCCCAAAAAAAAAAATGATGTACATATAAAGCTAATTGATAAGATAAGAGCATTTCCAAATTGAATATTAAATTAGAGAGTTGATATAGCTGATAATGGAAGGTTGAAGACAAGCAATGATTGTAATATGAATGATTTTGTCGCATTAGTGGATTAGGAAAGGTAGTTATCATTTTTTGGGTGGATGGGTCCTCCTTCATTTTACAGGATGTAAATTATAAATTGTTAAAATGAAACTTTATATTGTTTCTAAATAAACGTTTCATATAACAAAGTTGTTAAAATAAGAAAAACAAATCAGTACTTATGACGTTTTATTCATGTAATTAAATAATATATTAACTTATTTTATAAACCTCATATTATTATTAAATGTTACGAAAAGCAATAAATTAGAAGAATAGTTTGACCCATCAACATATAAGTCGGTATTAAATAATTTAACTTCAATAAAGTTTTCTACTAAAGTTTATACATTCATCGAATTATTGTCTCATTTTTTATTTATTTATTGTCTCATTTTTTTCTTTATTTATTCTCTGCTCTATTTTCTAATTCCAAGTACCAAACTTAACTCTTGCTCCTAAATACGTGGAAGGAGTTTGTTAAATTGTAATAATAGAAATTTTTGACGTTAAATAATATGAAATAGAAAGCTAATCACAGGGTGACGTGTGTAATTCAACTGTAAGGCCCATGCTGATGGGAAATGGAGTTGAAAATGGCACAAATTTCGAACCAGTGTCTTTTATGTTCTCGTATTGCCCGTTGGCAGTAAGTAACTAAACTAGAATTATAAGGAAAAAAAGACAAAAAAAAAAAAAAAAAAGTGGTATATTTTTCAAAAATCAAATAACAAAATATGTCGTATTCAATATTTATTTGGCGCCGGAATTCAAGATATTTTATAACATACTTCCAAATACCATACAATACTGAACCTTGCACGTGACCCCATTGCAGAACACGTGATGGTGTAGTTAACAGCGTACCTGGGCAATCATCTGCAAAACACAACCACGTGTCTCATCATCTTCTACGTACTTGCCTCGTCATC

mRNA sequence

ATGGCTGTCGCCAATGAGAAATCAGGTCACGATGAGAAGGTTTGGACCCTTTGTAGTATTCCCTTTTGGCAAAACAGCAATGCTCCGTCTTCTGCTTCTTCATCGTCTCTTATTCATAGAGTTCATTTGCAAAGCCACAGTCATAAGCCAGCAGAACATTCGAGTTCGCATCCTGGTGTGGTTTCGGCAATTGCCAAATCTCTACTTCCTACCAAAAGAAGACTTCGTCTCGATCCTTCCAACAAGCTCTACTTTCCATGTATGTCTCCGGTGGCTCTTTTCTCTTGTGCTTGGTTGATTTTCTGCCTCGTGGGGTACTTGGTTTTTGGTTTATTTATAGGGTTTAGTGTTTCCTTGATTAAGGTCAAGAGTGCTATTGAGATAAAGAATACGAGCAAGAATCATGTGGCTTTCAAATTCCAAACGACCGCACCGAAGAGCTGTTACATGCGTCCTCCTGGTGGTATACTTGCTCCTGATGAAAGTATTATTGCAACGGTATTCAAGTTTGTGGAGCCACTAGATAACAATGACAAGCATGTAGATCATAAGAGAAGGGTCAAGTTCAAGATTGTGAGCTTGAAAGTGAAGGGCGACATGGACTACGTGCCTGAGCTGTTCGATGAGCAAAAAGATCATGTAGCAGTTGAGCAAATATTGCAAGTTGTTTTTCTTGACCCGGATCATTCTAATCCTGCACTAGAGAAAGTCTTTCGACAACTAGCTGAGGCTGAGGCTGCAATTGAGACACGCCAAAAGCCTGCTGAAGAAGCAGACCCGAAAATTGTTGGTGGAGGACTTGTCCTCGACGAATGGAAAGAAAGAAGGGAAAGATATCTAGCTAAGCAACAGGTTGGAGGTGTGGGTTTGGTGTAGAATTCAGTTGCTTGTGTTATGGAAGTTGAAATCACATTCATGTGTGAACTTTTCATTGAGCCATTTGTCGAAGGAGCAACCATCGACGTTAGCAATCAAAGAACACGTGATGGTGTAGTTAACAGCGTACCTGGGCAATCATCTGCAAAACACAACCACGTGTCTCATCATCTTCTACGTACTTGCCTCGTCATC

Coding sequence (CDS)

ATGGCTGTCGCCAATGAGAAATCAGGTCACGATGAGAAGGTTTGGACCCTTTGTAGTATTCCCTTTTGGCAAAACAGCAATGCTCCGTCTTCTGCTTCTTCATCGTCTCTTATTCATAGAGTTCATTTGCAAAGCCACAGTCATAAGCCAGCAGAACATTCGAGTTCGCATCCTGGTGTGGTTTCGGCAATTGCCAAATCTCTACTTCCTACCAAAAGAAGACTTCGTCTCGATCCTTCCAACAAGCTCTACTTTCCATGTATGTCTCCGGTGGCTCTTTTCTCTTGTGCTTGGTTGATTTTCTGCCTCGTGGGGTACTTGGTTTTTGGTTTATTTATAGGGTTTAGTGTTTCCTTGATTAAGGTCAAGAGTGCTATTGAGATAAAGAATACGAGCAAGAATCATGTGGCTTTCAAATTCCAAACGACCGCACCGAAGAGCTGTTACATGCGTCCTCCTGGTGGTATACTTGCTCCTGATGAAAGTATTATTGCAACGGTATTCAAGTTTGTGGAGCCACTAGATAACAATGACAAGCATGTAGATCATAAGAGAAGGGTCAAGTTCAAGATTGTGAGCTTGAAAGTGAAGGGCGACATGGACTACGTGCCTGAGCTGTTCGATGAGCAAAAAGATCATGTAGCAGTTGAGCAAATATTGCAAGTTGTTTTTCTTGACCCGGATCATTCTAATCCTGCACTAGAGAAAGTCTTTCGACAACTAGCTGAGGCTGAGGCTGCAATTGAGACACGCCAAAAGCCTGCTGAAGAAGCAGACCCGAAAATTGTTGGTGGAGGACTTGTCCTCGACGAATGGAAAGAAAGAAGGGAAAGATATCTAGCTAAGCAACAGGTTGGAGGTGTGGGTTTGGTGTAG

Protein sequence

MAVANEKSGHDEKVWTLCSIPFWQNSNAPSSASSSSLIHRVHLQSHSHKPAEHSSSHPGVVSAIAKSLLPTKRRLRLDPSNKLYFPCMSPVALFSCAWLIFCLVGYLVFGLFIGFSVSLIKVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKGDMDYVPELFDEQKDHVAVEQILQVVFLDPDHSNPALEKVFRQLAEAEAAIETRQKPAEEADPKIVGGGLVLDEWKERRERYLAKQQVGGVGLV
Homology
BLAST of Lsi10G008610 vs. ExPASy Swiss-Prot
Match: Q8VYN2 (Vesicle-associated protein 4-2 OS=Arabidopsis thaliana OX=3702 GN=PVA42 PE=1 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 6.9e-79
Identity = 166/301 (55.15%), Postives = 199/301 (66.11%), Query Frame = 0

Query: 8   SGHDEKVWTLCSIPFWQNSNAPSSASSSSLIHRVHLQSHSHKPAEH-------------- 67
           SGH     +  + PF    +  SS+S+SS +H  H   H H    H              
Sbjct: 27  SGHRNAASSAATSPF---PSGASSSSTSSHLHNHHQHHHQHHHQHHHQLGYNGPHGDGSG 86

Query: 68  SSSHP---GVVSAIAKSLLPTKRRLRLDPSNKLYFPCMSPVALFSCAWLIFCLVGYLVFG 127
            + HP     VS++AKS LPTKRRL+LDPS KLYFP   P                    
Sbjct: 87  QNQHPTPSPSVSSVAKSFLPTKRRLKLDPSEKLYFP-YEPGK------------------ 146

Query: 128 LFIGFSVSLIKVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKF 187
                     +V+SAI+IKNTSK+HVAFKFQTTAPKSC+MRPPG ILAP E+IIATVFKF
Sbjct: 147 ----------QVRSAIKIKNTSKSHVAFKFQTTAPKSCFMRPPGAILAPGETIIATVFKF 206

Query: 188 VEPLDNNDKHVDHKRRVKFKIVSLKVKGDMDYVPELFDEQKDHVAVEQILQVVFLDPDHS 247
           VEP +NN+K +D + RVKFKI+SLKVKG MDYVPELFDEQKD V+ EQIL+V+FLDP+ S
Sbjct: 207 VEPPENNEKPMDQRSRVKFKIMSLKVKGPMDYVPELFDEQKDDVSKEQILRVIFLDPERS 266

Query: 248 NPALEKVFRQLAEAEAAIETRQKPAEEADPKIVGGGLVLDEWKERRERYLAKQQVGGVGL 292
           NPALEK+ RQLAEA+AA+E R+KP EE  PK++G GLV+DEWKERRERYLA+QQ  G   
Sbjct: 267 NPALEKLKRQLAEADAAVEARKKPPEETGPKMIGEGLVIDEWKERRERYLAQQQGEGADS 295

BLAST of Lsi10G008610 vs. ExPASy Swiss-Prot
Match: Q1ECE0 (Vesicle-associated protein 4-1 OS=Arabidopsis thaliana OX=3702 GN=PVA41 PE=2 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 5.9e-70
Identity = 147/280 (52.50%), Postives = 194/280 (69.29%), Query Frame = 0

Query: 12  EKVWTLCSIPFWQNSNAPSSASSSSLIHRVHLQSHSHKPAEHS--SSHPGVVSAIAKSLL 71
           + ++ LC  PFWQ  +  SS+S+ +  ++ +   H ++  + S  S  P  +S++A+SLL
Sbjct: 15  KNLFRLC--PFWQRRSTTSSSSTQN-PNQNYRSRHGNRNTDISAVSKPPLTMSSVARSLL 74

Query: 72  PTKRRLRLDPSNKLYFPCMSPVALFSCAWLIFCLVGYLVFGLFIGFSVSLIKVKSAIEIK 131
           P +RRLRLDPS+ LYFP   P                              +V+SAI++K
Sbjct: 75  PARRRLRLDPSSYLYFP-YEPGK----------------------------QVRSAIKLK 134

Query: 132 NTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDNNDKH-VDHKRRVK 191
           NTSK+H AFKFQTTAPKSCYMRPPGG+LAP ES+ ATVFKFVE  +NN+K  ++ K +VK
Sbjct: 135 NTSKSHTAFKFQTTAPKSCYMRPPGGVLAPGESVFATVFKFVEHPENNEKQPLNQKSKVK 194

Query: 192 FKIVSLKVKGDMDYVPELFDEQKDHVAVEQILQVVFLDPDHSNPALEKVFRQLAEAEAAI 251
           FKI+SLKVK  ++YVPELFDEQKD VAVEQ+L+V+F+D D  + ALEK+ RQL EAEAA+
Sbjct: 195 FKIMSLKVKPGVEYVPELFDEQKDQVAVEQVLRVIFIDADRPSAALEKLKRQLDEAEAAV 254

Query: 252 ETRQKPAEEADPKIVGGGLVLDEWKERRERYLAKQQVGGV 289
           E R+KP  E  P++VG GLV+DEWKERRE+YLA+QQV  V
Sbjct: 255 EARKKPPPETGPRVVGEGLVIDEWKERREKYLARQQVESV 262

BLAST of Lsi10G008610 vs. ExPASy Swiss-Prot
Match: Q8LPQ7 (Vesicle-associated protein 4-3 OS=Arabidopsis thaliana OX=3702 GN=PVA43 PE=2 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 4.2e-68
Identity = 157/314 (50.00%), Postives = 200/314 (63.69%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFW-QNSNAPSSASSSSL------IHRVHL-QSHSHKPAE 60
           MA+  +KS  D + W    +PF   NS APS++SSSS+      +   HL Q++ H+   
Sbjct: 1   MALTEDKSDSDGRRWGKFKLPFRNSNSQAPSASSSSSMATSSSSVTSSHLNQNYIHQSRH 60

Query: 61  HSSSHPGVV-----------------SAIAKSLLPTKRRLRLDPSNKLYFPCMSPVALFS 120
                P VV                 S++A+SLLPTKRRL+LDPS KLYFP   P     
Sbjct: 61  FQYHGPPVVEGLGQNHHQSAATIPSMSSVARSLLPTKRRLKLDPSAKLYFP-YEPGK--- 120

Query: 121 CAWLIFCLVGYLVFGLFIGFSVSLIKVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGG 180
                                    +V+SAI+IKNTSK+HVAFKFQTT PKSC+MRP G 
Sbjct: 121 -------------------------QVRSAIKIKNTSKSHVAFKFQTTVPKSCFMRPAGA 180

Query: 181 ILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKGDMDYVPELFDEQKDHVA 240
           ILAP E IIATVFKFVEP +NN+K ++ K  VKFKI+SLK+K   DY+PELF+EQKDHV+
Sbjct: 181 ILAPGEEIIATVFKFVEPPENNEKPMEQKSGVKFKIMSLKMKVPTDYMPELFEEQKDHVS 240

Query: 241 VEQILQVVFLDPDHSNPALEKVFRQLAEAEAAIETRQKPAEE-ADPKIVGGGLVLDEWKE 289
            EQ+++VVFLDP++ N  +EK+  QLAEA+AA E R+K +E    PK +G GLV+DEWK+
Sbjct: 241 EEQVMRVVFLDPENPNSMMEKLKSQLAEADAADEARKKASEGIVGPKPIGEGLVIDEWKQ 284

BLAST of Lsi10G008610 vs. ExPASy Swiss-Prot
Match: Q9LVU1 (Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 5.6e-04
Identity = 42/169 (24.85%), Postives = 77/169 (45.56%), Query Frame = 0

Query: 126 IEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKR 185
           +++ N ++N+VAFK +TT+PK  ++RP  G++ P +S I  V    +     +   D + 
Sbjct: 31  LKVANKTENYVAFKVKTTSPKKYFVRPNTGVIQPWDSCIIRVTLQAQ----REYPPDMQC 90

Query: 186 RVKFKIVSLKV--KGDMDYVP-ELFDEQKDHVAVEQILQVVFLDPDHSNPALEK-VFRQL 245
           + KF + S  V    D+D +P + F +       E  L+V ++ P  +  + E       
Sbjct: 91  KDKFLLQSTIVPPHTDVDELPQDTFTKDSGKTLTECKLKVSYITPSTTQRSSESGATNGD 150

Query: 246 AEAEAAIETRQKPAEEADPKIVGGGLVLDEWKERRERYLAKQQVGGVGL 291
            ++   I T Q+  EE D  +     +  E +  R R   +    G+ L
Sbjct: 151 GQSSETISTIQRLKEERDAAVKQTQQLQHELETVRRRRNQRNSGNGLSL 195

BLAST of Lsi10G008610 vs. ExPASy Swiss-Prot
Match: B9DHD7 (Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 7.3e-04
Identity = 39/122 (31.97%), Postives = 58/122 (47.54%), Query Frame = 0

Query: 115 FSVSLIKVKS-AIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVF--KFV 174
           F+V L K  S  +++ NT+ ++VAFK +TT+PK   +RP  G++AP  +   TV    F 
Sbjct: 15  FAVDLKKQTSCVVQLTNTTHHYVAFKVKTTSPKKYCVRPNVGVVAPKSTCEFTVIMQAFK 74

Query: 175 EPLDNNDKHVDHKRRVKFKIVSLKVKG---DMDYVPELFDEQKDHVAVEQILQVVFLDPD 231
           EP        D   + KF I S  V     D D    +F + +     E  L+V  + P 
Sbjct: 75  EP------PPDMVCKDKFLIQSTAVSAETTDEDITASMFSKAEGKHIEENKLRVTLVPPS 130

BLAST of Lsi10G008610 vs. ExPASy TrEMBL
Match: A0A0A0KE43 (MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G123970 PE=4 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 1.4e-122
Identity = 237/295 (80.34%), Postives = 244/295 (82.71%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFWQNSNAP----SSASSSSLIHRVHLQSHSHKPAEHSSS 60
           MAVA+EKSG D KVW+LCSIPFWQNSNAP    SS+SSSS+IHRVHLQS+ HKPAEHSSS
Sbjct: 1   MAVADEKSGPDGKVWSLCSIPFWQNSNAPSSSSSSSSSSSVIHRVHLQSNGHKPAEHSSS 60

Query: 61  HPGVVSAIAKSLLPTKRRLRLDPSNKLYFPCMSPVALFSCAWLIFCLVGYLVFGLFIGFS 120
           HPGVVSAIAKSL PTKRRLRLDPSN LYFP   P                          
Sbjct: 61  HPGVVSAIAKSLCPTKRRLRLDPSNNLYFP-FEPGK------------------------ 120

Query: 121 VSLIKVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDN 180
               +VKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDN
Sbjct: 121 ----QVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDN 180

Query: 181 NDKHVDHKRRVKFKIVSLKVKGDMDYVPELFDEQKDHVAVEQILQVVFLDPDHSNPALEK 240
           NDKHVDHKRRVKFKIVSLKVK D+DYVPELFDEQKD VAVEQILQ VFLDPDHSNPALEK
Sbjct: 181 NDKHVDHKRRVKFKIVSLKVKSDLDYVPELFDEQKDRVAVEQILQAVFLDPDHSNPALEK 240

Query: 241 VFRQLAEAEAAIETRQKPAEEADPKIVGGGLVLDEWKERRERYLAKQQVGGVGLV 292
           V RQLAEAEAA E R KPAEE DPKIVGGGLVLDEWKERRERYLAKQQVGGV LV
Sbjct: 241 VCRQLAEAEAAAEMRHKPAEETDPKIVGGGLVLDEWKERRERYLAKQQVGGVDLV 266

BLAST of Lsi10G008610 vs. ExPASy TrEMBL
Match: A0A5D3BT76 (Vesicle-associated protein 4-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold388G00900 PE=4 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 2.0e-121
Identity = 236/294 (80.27%), Postives = 242/294 (82.31%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFWQNSNAP---SSASSSSLIHRVHLQSHSHKPAEHSSSH 60
           MAVA+EKSG D KVWTLCSIPFWQN+NAP   SS+SSSS+IHRVHLQ + HKPAEHSSS 
Sbjct: 1   MAVADEKSGPDGKVWTLCSIPFWQNNNAPSSSSSSSSSSVIHRVHLQINGHKPAEHSSSQ 60

Query: 61  PGVVSAIAKSLLPTKRRLRLDPSNKLYFPCMSPVALFSCAWLIFCLVGYLVFGLFIGFSV 120
           PGVVSAIAKSL PTKRRLRLDPSNKLYFP   P                           
Sbjct: 61  PGVVSAIAKSLRPTKRRLRLDPSNKLYFP-FEPGK------------------------- 120

Query: 121 SLIKVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDNN 180
              +VKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDES IATVFKFVEPLDNN
Sbjct: 121 ---QVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESTIATVFKFVEPLDNN 180

Query: 181 DKHVDHKRRVKFKIVSLKVKGDMDYVPELFDEQKDHVAVEQILQVVFLDPDHSNPALEKV 240
           DKHVDHKRRVKFKIVSLKVK DMDYVPELFDEQKD VAVEQILQ VFLDPDHSNPALEKV
Sbjct: 181 DKHVDHKRRVKFKIVSLKVKSDMDYVPELFDEQKDRVAVEQILQAVFLDPDHSNPALEKV 240

Query: 241 FRQLAEAEAAIETRQKPAEEADPKIVGGGLVLDEWKERRERYLAKQQVGGVGLV 292
            RQLAEAEAA E R KPAEE DPKIVGGGLVLDEWKERRERYLAKQQVGGV LV
Sbjct: 241 CRQLAEAEAAAEMRHKPAEETDPKIVGGGLVLDEWKERRERYLAKQQVGGVDLV 265

BLAST of Lsi10G008610 vs. ExPASy TrEMBL
Match: A0A1S3BJS8 (vesicle-associated protein 4-2-like OS=Cucumis melo OX=3656 GN=LOC103490476 PE=4 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 2.0e-121
Identity = 236/294 (80.27%), Postives = 242/294 (82.31%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFWQNSNAP---SSASSSSLIHRVHLQSHSHKPAEHSSSH 60
           MAVA+EKSG D KVWTLCSIPFWQN+NAP   SS+SSSS+IHRVHLQ + HKPAEHSSS 
Sbjct: 1   MAVADEKSGPDGKVWTLCSIPFWQNNNAPSSSSSSSSSSVIHRVHLQINGHKPAEHSSSQ 60

Query: 61  PGVVSAIAKSLLPTKRRLRLDPSNKLYFPCMSPVALFSCAWLIFCLVGYLVFGLFIGFSV 120
           PGVVSAIAKSL PTKRRLRLDPSNKLYFP   P                           
Sbjct: 61  PGVVSAIAKSLRPTKRRLRLDPSNKLYFP-FEPGK------------------------- 120

Query: 121 SLIKVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDNN 180
              +VKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDES IATVFKFVEPLDNN
Sbjct: 121 ---QVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESTIATVFKFVEPLDNN 180

Query: 181 DKHVDHKRRVKFKIVSLKVKGDMDYVPELFDEQKDHVAVEQILQVVFLDPDHSNPALEKV 240
           DKHVDHKRRVKFKIVSLKVK DMDYVPELFDEQKD VAVEQILQ VFLDPDHSNPALEKV
Sbjct: 181 DKHVDHKRRVKFKIVSLKVKSDMDYVPELFDEQKDRVAVEQILQAVFLDPDHSNPALEKV 240

Query: 241 FRQLAEAEAAIETRQKPAEEADPKIVGGGLVLDEWKERRERYLAKQQVGGVGLV 292
            RQLAEAEAA E R KPAEE DPKIVGGGLVLDEWKERRERYLAKQQVGGV LV
Sbjct: 241 CRQLAEAEAAAEMRHKPAEETDPKIVGGGLVLDEWKERRERYLAKQQVGGVDLV 265

BLAST of Lsi10G008610 vs. ExPASy TrEMBL
Match: A0A5A7U105 (Vesicle-associated protein 4-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold133G001980 PE=4 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 9.9e-121
Identity = 235/294 (79.93%), Postives = 241/294 (81.97%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFWQNSNAP---SSASSSSLIHRVHLQSHSHKPAEHSSSH 60
           MAVA+EKSG D KVWTLCSIPFWQN+NAP   SS+SSSS+IHRVHLQ + HKPAEHSSS 
Sbjct: 1   MAVADEKSGPDGKVWTLCSIPFWQNNNAPSSSSSSSSSSVIHRVHLQINGHKPAEHSSSQ 60

Query: 61  PGVVSAIAKSLLPTKRRLRLDPSNKLYFPCMSPVALFSCAWLIFCLVGYLVFGLFIGFSV 120
           PGVVSAIAKSL PTKRRLRLDPSNKLYFP   P                           
Sbjct: 61  PGVVSAIAKSLRPTKRRLRLDPSNKLYFP-FEPGK------------------------- 120

Query: 121 SLIKVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDNN 180
              +VKSAIEIKNTSK HVAFKFQTTAPKSCYMRPPGGILAPDES IATVFKFVEPLDNN
Sbjct: 121 ---QVKSAIEIKNTSKTHVAFKFQTTAPKSCYMRPPGGILAPDESTIATVFKFVEPLDNN 180

Query: 181 DKHVDHKRRVKFKIVSLKVKGDMDYVPELFDEQKDHVAVEQILQVVFLDPDHSNPALEKV 240
           DKHVDHKRRVKFKIVSLKVK DMDYVPELFDEQKD VAVEQILQ VFLDPDHSNPALEKV
Sbjct: 181 DKHVDHKRRVKFKIVSLKVKSDMDYVPELFDEQKDRVAVEQILQAVFLDPDHSNPALEKV 240

Query: 241 FRQLAEAEAAIETRQKPAEEADPKIVGGGLVLDEWKERRERYLAKQQVGGVGLV 292
            RQLAEAEAA E R KPAEE DPKIVGGGLVLDEWKERRERYLAKQQVGGV LV
Sbjct: 241 CRQLAEAEAAAEMRHKPAEETDPKIVGGGLVLDEWKERRERYLAKQQVGGVDLV 265

BLAST of Lsi10G008610 vs. ExPASy TrEMBL
Match: A0A6J1I192 (vesicle-associated protein 4-2-like OS=Cucurbita maxima OX=3661 GN=LOC111469594 PE=4 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 3.5e-110
Identity = 218/293 (74.40%), Postives = 237/293 (80.89%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFWQNSN-APSSASSSSLIHRVHLQSHSHKPAEHSSSHP- 60
           MAVA++KS  D KVW+LCSIPFWQN N + SS SSS+ IHRV+ QS+ HKPAE+S S+P 
Sbjct: 1   MAVADQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPAENSGSYPS 60

Query: 61  GVVSAIAKSLLPTKRRLRLDPSNKLYFPCMSPVALFSCAWLIFCLVGYLVFGLFIGFSVS 120
           G VS IAKSLLPT+RRLRLDPSNKLYFP   P                            
Sbjct: 61  GKVSFIAKSLLPTRRRLRLDPSNKLYFP-YEPGK-------------------------- 120

Query: 121 LIKVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDNND 180
             +VKSAIEI+NTSKNHVAFKFQTTAPKSCYMRPPGGILAP ESIIATVFKFVEPL+NN+
Sbjct: 121 --QVKSAIEIQNTSKNHVAFKFQTTAPKSCYMRPPGGILAPGESIIATVFKFVEPLENNE 180

Query: 181 KHVDHKRRVKFKIVSLKVKGDMDYVPELFDEQKDHVAVEQILQVVFLDPDHSNPALEKVF 240
           K VDHKRRVKFKIVSLKVKG+MDYVPELFDEQ+DHVAVEQILQVVFLDP HSNPALEK+F
Sbjct: 181 KPVDHKRRVKFKIVSLKVKGNMDYVPELFDEQRDHVAVEQILQVVFLDPQHSNPALEKLF 240

Query: 241 RQLAEAEAAIETRQKPAEEADPKIVGGGLVLDEWKERRERYLAKQQVGGVGLV 292
           RQLA AEAA E RQKPAEE DPK+VG GLV+DEWKERRERYLAKQQVGGV LV
Sbjct: 241 RQLAVAEAATEVRQKPAEETDPKVVGEGLVIDEWKERRERYLAKQQVGGVDLV 264

BLAST of Lsi10G008610 vs. NCBI nr
Match: XP_011656923.2 (vesicle-associated protein 4-2 [Cucumis sativus] >KGN46696.2 hypothetical protein Csa_020729 [Cucumis sativus])

HSP 1 Score: 448.4 bits (1152), Expect = 4.9e-122
Identity = 237/297 (79.80%), Postives = 244/297 (82.15%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFWQNSNAP------SSASSSSLIHRVHLQSHSHKPAEHS 60
           MAVA+EKSG D KVW+LCSIPFWQNSNAP      SS+SSSS+IHRVHLQS+ HKPAEHS
Sbjct: 1   MAVADEKSGPDGKVWSLCSIPFWQNSNAPSSSSSSSSSSSSSVIHRVHLQSNGHKPAEHS 60

Query: 61  SSHPGVVSAIAKSLLPTKRRLRLDPSNKLYFPCMSPVALFSCAWLIFCLVGYLVFGLFIG 120
           SSHPGVVSAIAKSL PTKRRLRLDPSN LYFP   P                        
Sbjct: 61  SSHPGVVSAIAKSLCPTKRRLRLDPSNNLYFP-FEPGK---------------------- 120

Query: 121 FSVSLIKVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPL 180
                 +VKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPL
Sbjct: 121 ------QVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPL 180

Query: 181 DNNDKHVDHKRRVKFKIVSLKVKGDMDYVPELFDEQKDHVAVEQILQVVFLDPDHSNPAL 240
           DNNDKHVDHKRRVKFKIVSLKVK D+DYVPELFDEQKD VAVEQILQ VFLDPDHSNPAL
Sbjct: 181 DNNDKHVDHKRRVKFKIVSLKVKSDLDYVPELFDEQKDRVAVEQILQAVFLDPDHSNPAL 240

Query: 241 EKVFRQLAEAEAAIETRQKPAEEADPKIVGGGLVLDEWKERRERYLAKQQVGGVGLV 292
           EKV RQLAEAEAA E R KPAEE DPKIVGGGLVLDEWKERRERYLAKQQVGGV LV
Sbjct: 241 EKVCRQLAEAEAAAEMRHKPAEETDPKIVGGGLVLDEWKERRERYLAKQQVGGVDLV 268

BLAST of Lsi10G008610 vs. NCBI nr
Match: XP_038900695.1 (vesicle-associated protein 4-2-like [Benincasa hispida])

HSP 1 Score: 446.4 bits (1147), Expect = 1.8e-121
Identity = 234/282 (82.98%), Postives = 242/282 (85.82%), Query Frame = 0

Query: 11  DEKVWTLCSIPFWQNSNAP-SSASSSSLIHRVHLQSHSHKPAEHSSSHPGVVSAIAKSLL 70
           DEKV TLCSIPFWQNSNAP SS+SSSS+IHRVHLQS+ HKPAEHSSSHPGVVS+IAKSLL
Sbjct: 5   DEKVLTLCSIPFWQNSNAPSSSSSSSSVIHRVHLQSNGHKPAEHSSSHPGVVSSIAKSLL 64

Query: 71  PTKRRLRLDPSNKLYFPCMSPVALFSCAWLIFCLVGYLVFGLFIGFSVSLIKVKSAIEIK 130
           PTKRRLRLDPSNKLYFP   P                              +VKSAIEIK
Sbjct: 65  PTKRRLRLDPSNKLYFP-FEPGK----------------------------QVKSAIEIK 124

Query: 131 NTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKF 190
           NTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHK+RVKF
Sbjct: 125 NTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDNNDKHVDHKKRVKF 184

Query: 191 KIVSLKVKGDMDYVPELFDEQKDHVAVEQILQVVFLDPDHSNPALEKVFRQLAEAEAAIE 250
           KIVSLKVKG+MDYVPELFDEQ+DHVAVEQILQVVFLDPDHSNPALEKVFRQLAEAEAA E
Sbjct: 185 KIVSLKVKGNMDYVPELFDEQRDHVAVEQILQVVFLDPDHSNPALEKVFRQLAEAEAATE 244

Query: 251 TRQKPAEEADPKIVGGGLVLDEWKERRERYLAKQQVGGVGLV 292
            RQKPAEE DPKIVGGGLVLDEWKERRERYLAKQQVGGV LV
Sbjct: 245 MRQKPAEETDPKIVGGGLVLDEWKERRERYLAKQQVGGVDLV 257

BLAST of Lsi10G008610 vs. NCBI nr
Match: XP_008448221.1 (PREDICTED: vesicle-associated protein 4-2-like [Cucumis melo] >TYK02188.1 vesicle-associated protein 4-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 445.3 bits (1144), Expect = 4.1e-121
Identity = 236/294 (80.27%), Postives = 242/294 (82.31%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFWQNSNAP---SSASSSSLIHRVHLQSHSHKPAEHSSSH 60
           MAVA+EKSG D KVWTLCSIPFWQN+NAP   SS+SSSS+IHRVHLQ + HKPAEHSSS 
Sbjct: 1   MAVADEKSGPDGKVWTLCSIPFWQNNNAPSSSSSSSSSSVIHRVHLQINGHKPAEHSSSQ 60

Query: 61  PGVVSAIAKSLLPTKRRLRLDPSNKLYFPCMSPVALFSCAWLIFCLVGYLVFGLFIGFSV 120
           PGVVSAIAKSL PTKRRLRLDPSNKLYFP   P                           
Sbjct: 61  PGVVSAIAKSLRPTKRRLRLDPSNKLYFP-FEPGK------------------------- 120

Query: 121 SLIKVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDNN 180
              +VKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDES IATVFKFVEPLDNN
Sbjct: 121 ---QVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESTIATVFKFVEPLDNN 180

Query: 181 DKHVDHKRRVKFKIVSLKVKGDMDYVPELFDEQKDHVAVEQILQVVFLDPDHSNPALEKV 240
           DKHVDHKRRVKFKIVSLKVK DMDYVPELFDEQKD VAVEQILQ VFLDPDHSNPALEKV
Sbjct: 181 DKHVDHKRRVKFKIVSLKVKSDMDYVPELFDEQKDRVAVEQILQAVFLDPDHSNPALEKV 240

Query: 241 FRQLAEAEAAIETRQKPAEEADPKIVGGGLVLDEWKERRERYLAKQQVGGVGLV 292
            RQLAEAEAA E R KPAEE DPKIVGGGLVLDEWKERRERYLAKQQVGGV LV
Sbjct: 241 CRQLAEAEAAAEMRHKPAEETDPKIVGGGLVLDEWKERRERYLAKQQVGGVDLV 265

BLAST of Lsi10G008610 vs. NCBI nr
Match: KAA0047916.1 (vesicle-associated protein 4-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 443.0 bits (1138), Expect = 2.0e-120
Identity = 235/294 (79.93%), Postives = 241/294 (81.97%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFWQNSNAP---SSASSSSLIHRVHLQSHSHKPAEHSSSH 60
           MAVA+EKSG D KVWTLCSIPFWQN+NAP   SS+SSSS+IHRVHLQ + HKPAEHSSS 
Sbjct: 1   MAVADEKSGPDGKVWTLCSIPFWQNNNAPSSSSSSSSSSVIHRVHLQINGHKPAEHSSSQ 60

Query: 61  PGVVSAIAKSLLPTKRRLRLDPSNKLYFPCMSPVALFSCAWLIFCLVGYLVFGLFIGFSV 120
           PGVVSAIAKSL PTKRRLRLDPSNKLYFP   P                           
Sbjct: 61  PGVVSAIAKSLRPTKRRLRLDPSNKLYFP-FEPGK------------------------- 120

Query: 121 SLIKVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDNN 180
              +VKSAIEIKNTSK HVAFKFQTTAPKSCYMRPPGGILAPDES IATVFKFVEPLDNN
Sbjct: 121 ---QVKSAIEIKNTSKTHVAFKFQTTAPKSCYMRPPGGILAPDESTIATVFKFVEPLDNN 180

Query: 181 DKHVDHKRRVKFKIVSLKVKGDMDYVPELFDEQKDHVAVEQILQVVFLDPDHSNPALEKV 240
           DKHVDHKRRVKFKIVSLKVK DMDYVPELFDEQKD VAVEQILQ VFLDPDHSNPALEKV
Sbjct: 181 DKHVDHKRRVKFKIVSLKVKSDMDYVPELFDEQKDRVAVEQILQAVFLDPDHSNPALEKV 240

Query: 241 FRQLAEAEAAIETRQKPAEEADPKIVGGGLVLDEWKERRERYLAKQQVGGVGLV 292
            RQLAEAEAA E R KPAEE DPKIVGGGLVLDEWKERRERYLAKQQVGGV LV
Sbjct: 241 CRQLAEAEAAAEMRHKPAEETDPKIVGGGLVLDEWKERRERYLAKQQVGGVDLV 265

BLAST of Lsi10G008610 vs. NCBI nr
Match: XP_022970681.1 (vesicle-associated protein 4-2-like [Cucurbita maxima])

HSP 1 Score: 407.9 bits (1047), Expect = 7.3e-110
Identity = 218/293 (74.40%), Postives = 237/293 (80.89%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFWQNSN-APSSASSSSLIHRVHLQSHSHKPAEHSSSHP- 60
           MAVA++KS  D KVW+LCSIPFWQN N + SS SSS+ IHRV+ QS+ HKPAE+S S+P 
Sbjct: 1   MAVADQKSDLDGKVWSLCSIPFWQNGNVSSSSGSSSTAIHRVNEQSNGHKPAENSGSYPS 60

Query: 61  GVVSAIAKSLLPTKRRLRLDPSNKLYFPCMSPVALFSCAWLIFCLVGYLVFGLFIGFSVS 120
           G VS IAKSLLPT+RRLRLDPSNKLYFP   P                            
Sbjct: 61  GKVSFIAKSLLPTRRRLRLDPSNKLYFP-YEPGK-------------------------- 120

Query: 121 LIKVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDNND 180
             +VKSAIEI+NTSKNHVAFKFQTTAPKSCYMRPPGGILAP ESIIATVFKFVEPL+NN+
Sbjct: 121 --QVKSAIEIQNTSKNHVAFKFQTTAPKSCYMRPPGGILAPGESIIATVFKFVEPLENNE 180

Query: 181 KHVDHKRRVKFKIVSLKVKGDMDYVPELFDEQKDHVAVEQILQVVFLDPDHSNPALEKVF 240
           K VDHKRRVKFKIVSLKVKG+MDYVPELFDEQ+DHVAVEQILQVVFLDP HSNPALEK+F
Sbjct: 181 KPVDHKRRVKFKIVSLKVKGNMDYVPELFDEQRDHVAVEQILQVVFLDPQHSNPALEKLF 240

Query: 241 RQLAEAEAAIETRQKPAEEADPKIVGGGLVLDEWKERRERYLAKQQVGGVGLV 292
           RQLA AEAA E RQKPAEE DPK+VG GLV+DEWKERRERYLAKQQVGGV LV
Sbjct: 241 RQLAVAEAATEVRQKPAEETDPKVVGEGLVIDEWKERRERYLAKQQVGGVDLV 264

BLAST of Lsi10G008610 vs. TAIR 10
Match: AT4G21450.3 (PapD-like superfamily protein )

HSP 1 Score: 300.4 bits (768), Expect = 1.5e-81
Identity = 169/301 (56.15%), Postives = 205/301 (68.11%), Query Frame = 0

Query: 8   SGHDEKVWTLCSIPFWQNSNAPSSASSSSLIHRVHLQSHSHKPAEH-------------- 67
           SGH     +  + PF    +  SS+S+SS +H  H   H H    H              
Sbjct: 27  SGHRNAASSAATSPF---PSGASSSSTSSHLHNHHQHHHQHHHQHHHQLGYNGPHGDGSG 86

Query: 68  SSSHP---GVVSAIAKSLLPTKRRLRLDPSNKLYFPCMSPVALFSCAWLIFCLVGYLVFG 127
            + HP     VS++AKS LPTKRRL+LDPS KLYFP      L S    +          
Sbjct: 87  QNQHPTPSPSVSSVAKSFLPTKRRLKLDPSEKLYFPWFVSEDLRSIDTSL---------- 146

Query: 128 LFIGFSVSLIKVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKF 187
            F+ F+    +V+SAI+IKNTSK+HVAFKFQTTAPKSC+MRPPG ILAP E+IIATVFKF
Sbjct: 147 KFLSFNEPGKQVRSAIKIKNTSKSHVAFKFQTTAPKSCFMRPPGAILAPGETIIATVFKF 206

Query: 188 VEPLDNNDKHVDHKRRVKFKIVSLKVKGDMDYVPELFDEQKDHVAVEQILQVVFLDPDHS 247
           VEP +NN+K +D + RVKFKI+SLKVKG MDYVPELFDEQKD V+ EQIL+V+FLDP+ S
Sbjct: 207 VEPPENNEKPMDQRSRVKFKIMSLKVKGPMDYVPELFDEQKDDVSKEQILRVIFLDPERS 266

Query: 248 NPALEKVFRQLAEAEAAIETRQKPAEEADPKIVGGGLVLDEWKERRERYLAKQQVGGVGL 292
           NPALEK+ RQLAEA+AA+E R+KP EE  PK++G GLV+DEWKERRERYLA+QQ  G   
Sbjct: 267 NPALEKLKRQLAEADAAVEARKKPPEETGPKMIGEGLVIDEWKERRERYLAQQQGEGADS 314

BLAST of Lsi10G008610 vs. TAIR 10
Match: AT4G21450.1 (PapD-like superfamily protein )

HSP 1 Score: 295.4 bits (755), Expect = 4.9e-80
Identity = 166/301 (55.15%), Postives = 199/301 (66.11%), Query Frame = 0

Query: 8   SGHDEKVWTLCSIPFWQNSNAPSSASSSSLIHRVHLQSHSHKPAEH-------------- 67
           SGH     +  + PF    +  SS+S+SS +H  H   H H    H              
Sbjct: 27  SGHRNAASSAATSPF---PSGASSSSTSSHLHNHHQHHHQHHHQHHHQLGYNGPHGDGSG 86

Query: 68  SSSHP---GVVSAIAKSLLPTKRRLRLDPSNKLYFPCMSPVALFSCAWLIFCLVGYLVFG 127
            + HP     VS++AKS LPTKRRL+LDPS KLYFP   P                    
Sbjct: 87  QNQHPTPSPSVSSVAKSFLPTKRRLKLDPSEKLYFP-YEPGK------------------ 146

Query: 128 LFIGFSVSLIKVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKF 187
                     +V+SAI+IKNTSK+HVAFKFQTTAPKSC+MRPPG ILAP E+IIATVFKF
Sbjct: 147 ----------QVRSAIKIKNTSKSHVAFKFQTTAPKSCFMRPPGAILAPGETIIATVFKF 206

Query: 188 VEPLDNNDKHVDHKRRVKFKIVSLKVKGDMDYVPELFDEQKDHVAVEQILQVVFLDPDHS 247
           VEP +NN+K +D + RVKFKI+SLKVKG MDYVPELFDEQKD V+ EQIL+V+FLDP+ S
Sbjct: 207 VEPPENNEKPMDQRSRVKFKIMSLKVKGPMDYVPELFDEQKDDVSKEQILRVIFLDPERS 266

Query: 248 NPALEKVFRQLAEAEAAIETRQKPAEEADPKIVGGGLVLDEWKERRERYLAKQQVGGVGL 292
           NPALEK+ RQLAEA+AA+E R+KP EE  PK++G GLV+DEWKERRERYLA+QQ  G   
Sbjct: 267 NPALEKLKRQLAEADAAVEARKKPPEETGPKMIGEGLVIDEWKERRERYLAQQQGEGADS 295

BLAST of Lsi10G008610 vs. TAIR 10
Match: AT5G54110.1 (membrane-associated mannitol-induced )

HSP 1 Score: 265.8 bits (678), Expect = 4.2e-71
Identity = 147/280 (52.50%), Postives = 194/280 (69.29%), Query Frame = 0

Query: 12  EKVWTLCSIPFWQNSNAPSSASSSSLIHRVHLQSHSHKPAEHS--SSHPGVVSAIAKSLL 71
           + ++ LC  PFWQ  +  SS+S+ +  ++ +   H ++  + S  S  P  +S++A+SLL
Sbjct: 15  KNLFRLC--PFWQRRSTTSSSSTQN-PNQNYRSRHGNRNTDISAVSKPPLTMSSVARSLL 74

Query: 72  PTKRRLRLDPSNKLYFPCMSPVALFSCAWLIFCLVGYLVFGLFIGFSVSLIKVKSAIEIK 131
           P +RRLRLDPS+ LYFP   P                              +V+SAI++K
Sbjct: 75  PARRRLRLDPSSYLYFP-YEPGK----------------------------QVRSAIKLK 134

Query: 132 NTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKFVEPLDNNDKH-VDHKRRVK 191
           NTSK+H AFKFQTTAPKSCYMRPPGG+LAP ES+ ATVFKFVE  +NN+K  ++ K +VK
Sbjct: 135 NTSKSHTAFKFQTTAPKSCYMRPPGGVLAPGESVFATVFKFVEHPENNEKQPLNQKSKVK 194

Query: 192 FKIVSLKVKGDMDYVPELFDEQKDHVAVEQILQVVFLDPDHSNPALEKVFRQLAEAEAAI 251
           FKI+SLKVK  ++YVPELFDEQKD VAVEQ+L+V+F+D D  + ALEK+ RQL EAEAA+
Sbjct: 195 FKIMSLKVKPGVEYVPELFDEQKDQVAVEQVLRVIFIDADRPSAALEKLKRQLDEAEAAV 254

Query: 252 ETRQKPAEEADPKIVGGGLVLDEWKERRERYLAKQQVGGV 289
           E R+KP  E  P++VG GLV+DEWKERRE+YLA+QQV  V
Sbjct: 255 EARKKPPPETGPRVVGEGLVIDEWKERREKYLARQQVESV 262

BLAST of Lsi10G008610 vs. TAIR 10
Match: AT4G05060.1 (PapD-like superfamily protein )

HSP 1 Score: 259.6 bits (662), Expect = 3.0e-69
Identity = 157/314 (50.00%), Postives = 200/314 (63.69%), Query Frame = 0

Query: 1   MAVANEKSGHDEKVWTLCSIPFW-QNSNAPSSASSSSL------IHRVHL-QSHSHKPAE 60
           MA+  +KS  D + W    +PF   NS APS++SSSS+      +   HL Q++ H+   
Sbjct: 1   MALTEDKSDSDGRRWGKFKLPFRNSNSQAPSASSSSSMATSSSSVTSSHLNQNYIHQSRH 60

Query: 61  HSSSHPGVV-----------------SAIAKSLLPTKRRLRLDPSNKLYFPCMSPVALFS 120
                P VV                 S++A+SLLPTKRRL+LDPS KLYFP   P     
Sbjct: 61  FQYHGPPVVEGLGQNHHQSAATIPSMSSVARSLLPTKRRLKLDPSAKLYFP-YEPGK--- 120

Query: 121 CAWLIFCLVGYLVFGLFIGFSVSLIKVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGG 180
                                    +V+SAI+IKNTSK+HVAFKFQTT PKSC+MRP G 
Sbjct: 121 -------------------------QVRSAIKIKNTSKSHVAFKFQTTVPKSCFMRPAGA 180

Query: 181 ILAPDESIIATVFKFVEPLDNNDKHVDHKRRVKFKIVSLKVKGDMDYVPELFDEQKDHVA 240
           ILAP E IIATVFKFVEP +NN+K ++ K  VKFKI+SLK+K   DY+PELF+EQKDHV+
Sbjct: 181 ILAPGEEIIATVFKFVEPPENNEKPMEQKSGVKFKIMSLKMKVPTDYMPELFEEQKDHVS 240

Query: 241 VEQILQVVFLDPDHSNPALEKVFRQLAEAEAAIETRQKPAEE-ADPKIVGGGLVLDEWKE 289
            EQ+++VVFLDP++ N  +EK+  QLAEA+AA E R+K +E    PK +G GLV+DEWK+
Sbjct: 241 EEQVMRVVFLDPENPNSMMEKLKSQLAEADAADEARKKASEGIVGPKPIGEGLVIDEWKQ 284

BLAST of Lsi10G008610 vs. TAIR 10
Match: AT4G21450.2 (PapD-like superfamily protein )

HSP 1 Score: 177.6 bits (449), Expect = 1.5e-44
Identity = 107/216 (49.54%), Postives = 128/216 (59.26%), Query Frame = 0

Query: 8   SGHDEKVWTLCSIPFWQNSNAPSSASSSSLIHRVHLQSHSHKPAEH-------------- 67
           SGH     +  + PF    +  SS+S+SS +H  H   H H    H              
Sbjct: 27  SGHRNAASSAATSPF---PSGASSSSTSSHLHNHHQHHHQHHHQHHHQLGYNGPHGDGSG 86

Query: 68  SSSHP---GVVSAIAKSLLPTKRRLRLDPSNKLYFPCMSPVALFSCAWLIFCLVGYLVFG 127
            + HP     VS++AKS LPTKRRL+LDPS KLYFP   P                    
Sbjct: 87  QNQHPTPSPSVSSVAKSFLPTKRRLKLDPSEKLYFP-YEPGK------------------ 146

Query: 128 LFIGFSVSLIKVKSAIEIKNTSKNHVAFKFQTTAPKSCYMRPPGGILAPDESIIATVFKF 187
                     +V+SAI+IKNTSK+HVAFKFQTTAPKSC+MRPPG ILAP E+IIATVFKF
Sbjct: 147 ----------QVRSAIKIKNTSKSHVAFKFQTTAPKSCFMRPPGAILAPGETIIATVFKF 206

Query: 188 VEPLDNNDKHVDHKRRVKFKIVSLKVKGDMDYVPEL 207
           VEP +NN+K +D + RVKFKI+SLKVKG MDYVPEL
Sbjct: 207 VEPPENNEKPMDQRSRVKFKIMSLKVKGPMDYVPEL 210

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VYN26.9e-7955.15Vesicle-associated protein 4-2 OS=Arabidopsis thaliana OX=3702 GN=PVA42 PE=1 SV=... [more]
Q1ECE05.9e-7052.50Vesicle-associated protein 4-1 OS=Arabidopsis thaliana OX=3702 GN=PVA41 PE=2 SV=... [more]
Q8LPQ74.2e-6850.00Vesicle-associated protein 4-3 OS=Arabidopsis thaliana OX=3702 GN=PVA43 PE=2 SV=... [more]
Q9LVU15.6e-0424.85Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=... [more]
B9DHD77.3e-0431.97Vesicle-associated protein 2-2 OS=Arabidopsis thaliana OX=3702 GN=PVA22 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A0A0KE431.4e-12280.34MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G123970 PE=4 SV... [more]
A0A5D3BT762.0e-12180.27Vesicle-associated protein 4-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3BJS82.0e-12180.27vesicle-associated protein 4-2-like OS=Cucumis melo OX=3656 GN=LOC103490476 PE=4... [more]
A0A5A7U1059.9e-12179.93Vesicle-associated protein 4-2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6... [more]
A0A6J1I1923.5e-11074.40vesicle-associated protein 4-2-like OS=Cucurbita maxima OX=3661 GN=LOC111469594 ... [more]
Match NameE-valueIdentityDescription
XP_011656923.24.9e-12279.80vesicle-associated protein 4-2 [Cucumis sativus] >KGN46696.2 hypothetical protei... [more]
XP_038900695.11.8e-12182.98vesicle-associated protein 4-2-like [Benincasa hispida][more]
XP_008448221.14.1e-12180.27PREDICTED: vesicle-associated protein 4-2-like [Cucumis melo] >TYK02188.1 vesicl... [more]
KAA0047916.12.0e-12079.93vesicle-associated protein 4-2-like [Cucumis melo var. makuwa][more]
XP_022970681.17.3e-11074.40vesicle-associated protein 4-2-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G21450.31.5e-8156.15PapD-like superfamily protein [more]
AT4G21450.14.9e-8055.15PapD-like superfamily protein [more]
AT5G54110.14.2e-7152.50membrane-associated mannitol-induced [more]
AT4G05060.13.0e-6950.00PapD-like superfamily protein [more]
AT4G21450.21.5e-4449.54PapD-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 70..228
e-value: 2.2E-21
score: 78.1
IPR000535Major sperm protein (MSP) domainPFAMPF00635Motile_Spermcoord: 121..214
e-value: 7.7E-23
score: 80.4
IPR000535Major sperm protein (MSP) domainPROSITEPS50202MSPcoord: 74..225
score: 17.903418
NoneNo IPR availablePANTHERPTHR10809:SF101VESICLE-ASSOCIATED MEMBRANE-PROTEIN-ASSOCIATED PROTEIN-RELATEDcoord: 121..288
NoneNo IPR availablePANTHERPTHR10809:SF101VESICLE-ASSOCIATED MEMBRANE-PROTEIN-ASSOCIATED PROTEIN-RELATEDcoord: 35..86
IPR016763Vesicle-associated membrane-protein-associated proteinPANTHERPTHR10809VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEINcoord: 121..288
IPR016763Vesicle-associated membrane-protein-associated proteinPANTHERPTHR10809VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEINcoord: 35..86
IPR008962PapD-like superfamilySUPERFAMILY49354PapD-likecoord: 122..234

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G008610.1Lsi10G008610.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090158 endoplasmic reticulum membrane organization
biological_process GO:0061817 endoplasmic reticulum-plasma membrane tethering
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0005886 plasma membrane