Lsi10G008380 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi10G008380
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Calcium-dependent protein kinase
Location	chr10: 11959313 .. 11961466 (+)
RNA-Seq Expression	Lsi10G008380
Synteny	Lsi10G008380

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTACCCTCCTACCATTTATCCTATTTTTGGCGATTTTCCACCGGAAAACAAAAATAACAATAAAAAAATGTTCTTTCGGCTTCCCATTTTCGGGTGTAAAATTCAATCCCTTCCGTGAAGAACAAAATCCCGACAATCAAACAGCTTTACTGAGAATGGAGAATACGGGGATTCTAAAGGAGTGGTTTAATCGAGTCGATTCGGAGAAATCTGGAAGCATCACAGCTTCTCAGCTTCAGGTCAATGTTCATCTTCTTCTTCATTAGCTCTTATTGTTTTCGTCTCAAAAGTCCCAATCATTGACAAAATTTTCGGGTTTCCTCCATGATCAGAATGCTCTGGCGGTTGGAAACCTCAACTTTCCCCACTCAATTGTACAGCAAATGATCAGGTCTTCGAATTCTCCTCTTCTAATGATCTATCTAAAGAATTTTCTGCTGATTTTTTATTTTTATTTTTATTTCTTGTTAGAATGTATGATTTTGATAGAAATGGAACTATGAGCTTCGAAGGTAAGTAACATAGTATTTAAACCTACGGCTTTTGTTTACGTTTGAGAAGCGGTATTGATTTTCATGAGTTTTGTAATCTATGCCTCGTCTCCAAAAATTTGATGTTCTCTCTGATGTTGTAATGCATTTTTCTCAGAGTTTGTAGCTCTCAACAAGTTCCTCCTTAAGGTTAGTTTTCTAGATTATCTTTCTATCTTGTGGTAGTCTTGAATATTTAGTTTTTTCTTTAGTGAATTTAAAAAATCAATTTAATTAAAGCATCTGCTTTTCCTTCCATCTTCTGATTACTTTTTAATTTCTCTTCCAGCTTCAGCAAGCCTTCTCTGACTTCGAAAGGTGATTTTGTACTTTCTTTCTATAATTTTGATGACTACATGAACCCTATCTTGCTTCACACAATACATTCTATTAGAAGTTTTTGTTGTAAATTGCCTGGAGATTTACAAATAATTTGAAACCATTATTCGGGGCAGAGGCAGCTTCTGCTCTATATATGGCCAGATTGATTTCTGTCTCCATATCTGCTGTTGGCCAAATAAAGTATATACCATAAACAATTGTTACTAATTAGCCAGAACTCCTAGTTGCAGATGAATCAAACTTAGAGCGTTTAGTTTATATCTTGATGGATCTAAACACGTCTTCTCTTTGTTTGTTGTTACAGAGGTCGTGGTTATCTAGTCCCAGATGATGTATACGAGGTGTGTACTTGGTTTTAAGTATTAACTTCTATAGATCTATTAGGATCAATTTGGGAAGAATAATATTCACATTGCGTTTGCATCTAAAATTATTTACCTGCTGAAATATCCTTTCAGGCATTGGTTAAAATTGGCTTCACGCTGGACTCTCCGGCTTTTTATACTGTCTGTGAGGTAAAATTTTCATTTCACCATTGAAAATGGAAATAAAGAAATTTCAGTAGTCTTGGTAAATATTGTGGCCCACTTGTATGTGTTAAAAGCTTGTTTAAAAGTTTAAAGATTAGTTTAGTGGGAGTGATCAAGCTAATGAACTTCATGGTTGGAACTTTTCTTTAAATCTTCATCAGCAGTCCTTGACTACTTACTTCAGAGCCGTTAACTCTGGATGATTTACAATGTCAGCAATTAGTAAAAATTGCATGTACTGGGCGTTGAATCATAATAACCTTGACAGAAACTTGACGCATGAAGATACTTACATTCATAAGTGAAGTGTTAACAAGCAATGCTCACCTCCTCCCTCTAGAATACAGCATAAACCATTATTCTTCTGTAATCTTCCATTAGAGAGCCTTTTTCCATCTCTCCATTTTGTCTTTTTGGTGGTACCTTTCATCCTTCTAATTATCTTTTGGTAAAAGGAAATCTGTCTCGCCTTTCTTTTTATTCCATCTCCACAATGCTTTGTAAATGTGATTGGTTTGGTTTGCTGCTTTGAAAGTAGAACCTGTCTTTTCGAGATATTTAGTTATGCAGGACATATTGATTGTGAGTACGATGAATGGGTATCTATTCCCTGTAACTGTTTGGGGATCTGAACTTCTATTATATTTCTAATTTCTAAATATATTTGCAGAGCTTTGATCAAAAGAAGAATGGGAGATTTCGGCTAGACGATTTCATATCTCTATGTATATTTGTTCAATCAGCTGGGTAG

mRNA sequence

TTTACCCTCCTACCATTTATCCTATTTTTGGCGATTTTCCACCGGAAAACAAAAATAACAATAAAAAAATGTTCTTTCGGCTTCCCATTTTCGGGTGTAAAATTCAATCCCTTCCGTGAAGAACAAAATCCCGACAATCAAACAGCTTTACTGAGAATGGAGAATACGGGGATTCTAAAGGAGTGGTTTAATCGAGTCGATTCGGAGAAATCTGGAAGCATCACAGCTTCTCAGCTTCAGAATGCTCTGGCGGTTGGAAACCTCAACTTTCCCCACTCAATTGTACAGCAAATGATCAGAATGTATGATTTTGATAGAAATGGAACTATGAGCTTCGAAGAGTTTGTAGCTCTCAACAAGTTCCTCCTTAAGCTTCAGCAAGCCTTCTCTGACTTCGAAAGAGGTCGTGGTTATCTAGTCCCAGATGATGTATACGAGGCATTGGTTAAAATTGGCTTCACGCTGGACTCTCCGGCTTTTTATACTGTCTGTGAGAGCTTTGATCAAAAGAAGAATGGGAGATTTCGGCTAGACGATTTCATATCTCTATGTATATTTGTTCAATCAGCTGGGTAG

Coding sequence (CDS)

ATGGAGAATACGGGGATTCTAAAGGAGTGGTTTAATCGAGTCGATTCGGAGAAATCTGGAAGCATCACAGCTTCTCAGCTTCAGAATGCTCTGGCGGTTGGAAACCTCAACTTTCCCCACTCAATTGTACAGCAAATGATCAGAATGTATGATTTTGATAGAAATGGAACTATGAGCTTCGAAGAGTTTGTAGCTCTCAACAAGTTCCTCCTTAAGCTTCAGCAAGCCTTCTCTGACTTCGAAAGAGGTCGTGGTTATCTAGTCCCAGATGATGTATACGAGGCATTGGTTAAAATTGGCTTCACGCTGGACTCTCCGGCTTTTTATACTGTCTGTGAGAGCTTTGATCAAAAGAAGAATGGGAGATTTCGGCTAGACGATTTCATATCTCTATGTATATTTGTTCAATCAGCTGGGTAG

Protein sequence

MENTGILKEWFNRVDSEKSGSITASQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSFEEFVALNKFLLKLQQAFSDFERGRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKKNGRFRLDDFISLCIFVQSAG

Homology

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BLAST of Lsi10G008380 vs. ExPASy Swiss-Prot
Match: Q94743 (Sorcin OS=Schistosoma japonicum OX=6182 PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 4.0e-16
Identity = 51/138 (36.96%), Postives = 81/138 (58.70%), Query Frame = 0


Query: 3   NTGILKEWFNRVDSEKSGSITASQLQNALAVGNLNFPHSI--VQQMIRMYDFDRNGTMSF 62
           +T  L+  F+RVD++KSGSI+A++LQ +L+ G L  P +I  VQ M+ M+D D NGT++F
Sbjct: 4   DTNSLRHIFSRVDADKSGSISANELQTSLSNG-LGTPLNIRTVQLMVAMFDRDMNGTINF 63

Query: 63  EEFVALNKFLLKLQQAFSDFER-GRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKK 122
            EF+ L K++   Q  F  ++R   G +  ++   AL+  G+ L SP F  +      + 
Sbjct: 64  NEFLGLFKYVQDWQTCFRRYDRDNSGSIDLNEFSNALISFGYHL-SPQFVNLMMRRFDRN 123

Query: 123 NGRFRLDDFISLCIFVQS 138
            G    DDFI  C+ +Q+
Sbjct: 124 RGSIAFDDFIYACVCLQT 139

[Close]

BLAST of Lsi10G008380 vs. ExPASy Swiss-Prot
Match: Q06850 (Calcium-dependent protein kinase 1 OS=Arabidopsis thaliana OX=3702 GN=CPK1 PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.0e-12
Identity = 39/132 (29.55%), Postives = 73/132 (55.30%), Query Frame = 0


Query: 7   LKEWFNRVDSEKSGSITASQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSFEEFVAL 66
           LKE FN +D++KSG IT  +L+  L     N   S +  +++  D D +GT+ ++EF+A 
Sbjct: 456 LKEMFNMIDADKSGQITFEELKAGLKRVGANLKESEILDLMQAADVDNSGTIDYKEFIAA 515

Query: 67  NKFLLKLQQ------AFSDFER-GRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKK 126
              L K+++      AF+ F++ G GY+ PD++ +A  + G  ++      +    DQ  
Sbjct: 516 TLHLNKIEREDHLFAAFTYFDKDGSGYITPDELQQACEEFG--VEDVRIEELMRDVDQDN 575

Query: 127 NGRFRLDDFISL 132
           +GR   ++F+++
Sbjct: 576 DGRIDYNEFVAM 585

[Close]

BLAST of Lsi10G008380 vs. ExPASy Swiss-Prot
Match: Q38870 (Calcium-dependent protein kinase 2 OS=Arabidopsis thaliana OX=3702 GN=CPK2 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.1e-10
Identity = 36/132 (27.27%), Postives = 71/132 (53.79%), Query Frame = 0


Query: 7   LKEWFNRVDSEKSGSITASQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSFEEFVAL 66
           LK+ F  +D++ SG IT  +L+  L     N   S +  +++  D D +GT+ ++EF+A 
Sbjct: 492 LKQMFKMIDADNSGQITFEELKAGLKRVGANLKESEILDLMQAADVDNSGTIDYKEFIAA 551

Query: 67  NKFLLKLQQ------AFSDFERGR-GYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKK 126
              L K+++      AFS F++   G++ PD++ +A  + G  ++      +    DQ K
Sbjct: 552 TLHLNKIEREDHLFAAFSYFDKDESGFITPDELQQACEEFG--VEDARIEEMMRDVDQDK 611

Query: 127 NGRFRLDDFISL 132
           +GR   ++F+++
Sbjct: 612 DGRIDYNEFVAM 621

[Close]

BLAST of Lsi10G008380 vs. ExPASy Swiss-Prot
Match: Q2RAV0 (Calcium-dependent protein kinase 25 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK25 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.1e-10
Identity = 38/132 (28.79%), Postives = 67/132 (50.76%), Query Frame = 0


Query: 7   LKEWFNRVDSEKSGSITASQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSFEEFVAL 66
           LKE F  +D + SG+IT  +L+N LA     F  + ++Q++   D D NG + +EEFV  
Sbjct: 389 LKEMFKNIDKDNSGTITLEELKNGLAKQGTKFSDNEIEQLMEAADADGNGIIDYEEFVTA 448

Query: 67  NKFLLKLQQ------AFSDFER-GRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKK 126
              + K+ +      AF  F++   GY+  +++ +AL + G   D+     V    D   
Sbjct: 449 TVHMNKMDREEHLYTAFQYFDKDNSGYITKEELEQALKEQGL-YDANEIKDVITDADSNN 508

Query: 127 NGRFRLDDFISL 132
           +GR    +F+++
Sbjct: 509 DGRIDYSEFVAM 519

[Close]

BLAST of Lsi10G008380 vs. ExPASy Swiss-Prot
Match: Q2QY37 (Calcium-dependent protein kinase 26 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK26 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.1e-10
Identity = 38/132 (28.79%), Postives = 67/132 (50.76%), Query Frame = 0


Query: 7   LKEWFNRVDSEKSGSITASQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSFEEFVAL 66
           LKE F  +D + SG+IT  +L+N LA     F  + ++Q++   D D NG + +EEFV  
Sbjct: 389 LKEMFKNIDKDNSGTITLEELKNGLAKQGTKFSDNEIEQLMEAADADGNGIIDYEEFVTA 448

Query: 67  NKFLLKLQQ------AFSDFER-GRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKK 126
              + K+ +      AF  F++   GY+  +++ +AL + G   D+     V    D   
Sbjct: 449 TVHMNKMDREEHLYTAFQYFDKDNSGYITKEELEQALKEQGL-YDANEIKDVITDADSNN 508

Query: 127 NGRFRLDDFISL 132
           +GR    +F+++
Sbjct: 509 DGRIDYSEFVAM 519

[Close]

BLAST of Lsi10G008380 vs. ExPASy TrEMBL
Match: A0A0A0KAX1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G118890 PE=4 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 6.9e-72
Identity = 136/139 (97.84%), Postives = 137/139 (98.56%), Query Frame = 0


Query: 1   MENTGILKEWFNRVDSEKSGSITASQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF 60
           MENTGILKEWF+RVDSEKSGSITA QLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF
Sbjct: 1   MENTGILKEWFDRVDSEKSGSITAPQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF 60

Query: 61  EEFVALNKFLLKLQQAFSDFERGRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKKN 120
           EEFVALNKFLLKLQQAFSD ERGRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKKN
Sbjct: 61  EEFVALNKFLLKLQQAFSDLERGRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKKN 120

Query: 121 GRFRLDDFISLCIFVQSAG 140
           GRFRLDDFISLCIFVQSAG
Sbjct: 121 GRFRLDDFISLCIFVQSAG 139

[Close]

BLAST of Lsi10G008380 vs. ExPASy TrEMBL
Match: A0A1S4DX56 (sorcin-like OS=Cucumis melo OX=3656 GN=LOC103490457 PE=4 SV=1)

HSP 1 Score: 275.4 bits (703), Expect = 1.3e-70
Identity = 134/139 (96.40%), Postives = 135/139 (97.12%), Query Frame = 0


Query: 1   MENTGILKEWFNRVDSEKSGSITASQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF 60
           MENTGILKEWF+RVDS  SGSITA QLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF
Sbjct: 1   MENTGILKEWFDRVDSANSGSITAPQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF 60

Query: 61  EEFVALNKFLLKLQQAFSDFERGRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKKN 120
           EEFVALNKFLLKLQQAFSD ERGRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKKN
Sbjct: 61  EEFVALNKFLLKLQQAFSDLERGRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKKN 120

Query: 121 GRFRLDDFISLCIFVQSAG 140
           GRFRLDDFISLCIFVQSAG
Sbjct: 121 GRFRLDDFISLCIFVQSAG 139

[Close]

BLAST of Lsi10G008380 vs. ExPASy TrEMBL
Match: A0A6J1JFX0 (sorcin-like OS=Cucurbita maxima OX=3661 GN=LOC111484048 PE=4 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 2.1e-68
Identity = 131/138 (94.93%), Postives = 134/138 (97.10%), Query Frame = 0


Query: 1   MENTGILKEWFNRVDSEKSGSITASQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF 60
           MENTGILKEWF+RVDSEKSGSI A QLQNALAVGNLNFP SIVQQMIRMYDFDRNGTMSF
Sbjct: 1   MENTGILKEWFDRVDSEKSGSIIAPQLQNALAVGNLNFPLSIVQQMIRMYDFDRNGTMSF 60

Query: 61  EEFVALNKFLLKLQQAFSDFERGRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKKN 120
           EEFVALNKFL+KLQQAFSD ERGRGYLVP+DVYEALVKIGFTLDSPAFYTVCESFDQKKN
Sbjct: 61  EEFVALNKFLIKLQQAFSDLERGRGYLVPEDVYEALVKIGFTLDSPAFYTVCESFDQKKN 120

Query: 121 GRFRLDDFISLCIFVQSA 139
           GRFRLDDFISLCIFVQSA
Sbjct: 121 GRFRLDDFISLCIFVQSA 138

[Close]

BLAST of Lsi10G008380 vs. ExPASy TrEMBL
Match: A0A6J1CIP5 (sorcin-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011328 PE=4 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 2.1e-68
Identity = 130/139 (93.53%), Postives = 135/139 (97.12%), Query Frame = 0


Query: 1   MENTGILKEWFNRVDSEKSGSITASQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF 60
           MENTGILKEWF+RVDSEKSGS+TA QLQ ALAVGNLNFP SIVQQMIRMYDFDRNGTMSF
Sbjct: 1   MENTGILKEWFDRVDSEKSGSVTAPQLQKALAVGNLNFPLSIVQQMIRMYDFDRNGTMSF 60

Query: 61  EEFVALNKFLLKLQQAFSDFERGRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKKN 120
           EEFVALNKFLLKLQQAFSD ERGRG+LVP+DVYEALVKIG+TLDSPAFYTVCESFDQKKN
Sbjct: 61  EEFVALNKFLLKLQQAFSDLERGRGFLVPNDVYEALVKIGYTLDSPAFYTVCESFDQKKN 120

Query: 121 GRFRLDDFISLCIFVQSAG 140
           GRFRLDDFISLCIFVQSAG
Sbjct: 121 GRFRLDDFISLCIFVQSAG 139

[Close]

BLAST of Lsi10G008380 vs. ExPASy TrEMBL
Match: A0A6J1CGJ0 (sorcin-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011328 PE=4 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 2.1e-68
Identity = 130/139 (93.53%), Postives = 135/139 (97.12%), Query Frame = 0


Query: 1   MENTGILKEWFNRVDSEKSGSITASQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF 60
           MENTGILKEWF+RVDSEKSGS+TA QLQ ALAVGNLNFP SIVQQMIRMYDFDRNGTMSF
Sbjct: 1   MENTGILKEWFDRVDSEKSGSVTAPQLQKALAVGNLNFPLSIVQQMIRMYDFDRNGTMSF 60

Query: 61  EEFVALNKFLLKLQQAFSDFERGRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKKN 120
           EEFVALNKFLLKLQQAFSD ERGRG+LVP+DVYEALVKIG+TLDSPAFYTVCESFDQKKN
Sbjct: 61  EEFVALNKFLLKLQQAFSDLERGRGFLVPNDVYEALVKIGYTLDSPAFYTVCESFDQKKN 120

Query: 121 GRFRLDDFISLCIFVQSAG 140
           GRFRLDDFISLCIFVQSAG
Sbjct: 121 GRFRLDDFISLCIFVQSAG 139

[Close]

BLAST of Lsi10G008380 vs. NCBI nr
Match: XP_004139969.1 (sorcin isoform X2 [Cucumis sativus] >KGN46663.1 hypothetical protein Csa_005521 [Cucumis sativus])

HSP 1 Score: 279.6 bits (714), Expect = 1.4e-71
Identity = 136/139 (97.84%), Postives = 137/139 (98.56%), Query Frame = 0


Query: 1   MENTGILKEWFNRVDSEKSGSITASQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF 60
           MENTGILKEWF+RVDSEKSGSITA QLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF
Sbjct: 1   MENTGILKEWFDRVDSEKSGSITAPQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF 60

Query: 61  EEFVALNKFLLKLQQAFSDFERGRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKKN 120
           EEFVALNKFLLKLQQAFSD ERGRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKKN
Sbjct: 61  EEFVALNKFLLKLQQAFSDLERGRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKKN 120

Query: 121 GRFRLDDFISLCIFVQSAG 140
           GRFRLDDFISLCIFVQSAG
Sbjct: 121 GRFRLDDFISLCIFVQSAG 139

[Close]

BLAST of Lsi10G008380 vs. NCBI nr
Match: XP_011656916.1 (sorcin isoform X1 [Cucumis sativus])

HSP 1 Score: 279.6 bits (714), Expect = 1.4e-71
Identity = 136/139 (97.84%), Postives = 137/139 (98.56%), Query Frame = 0


Query: 1   MENTGILKEWFNRVDSEKSGSITASQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF 60
           MENTGILKEWF+RVDSEKSGSITA QLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF
Sbjct: 1   MENTGILKEWFDRVDSEKSGSITAPQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF 60

Query: 61  EEFVALNKFLLKLQQAFSDFERGRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKKN 120
           EEFVALNKFLLKLQQAFSD ERGRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKKN
Sbjct: 61  EEFVALNKFLLKLQQAFSDLERGRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKKN 120

Query: 121 GRFRLDDFISLCIFVQSAG 140
           GRFRLDDFISLCIFVQSAG
Sbjct: 121 GRFRLDDFISLCIFVQSAG 139

[Close]

BLAST of Lsi10G008380 vs. NCBI nr
Match: XP_038900411.1 (sorcin-like isoform X1 [Benincasa hispida])

HSP 1 Score: 276.2 bits (705), Expect = 1.6e-70
Identity = 134/139 (96.40%), Postives = 135/139 (97.12%), Query Frame = 0


Query: 1   MENTGILKEWFNRVDSEKSGSITASQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF 60
           MENTGILKEWFNRVDSE SGSITA QLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF
Sbjct: 3   MENTGILKEWFNRVDSENSGSITAPQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF 62

Query: 61  EEFVALNKFLLKLQQAFSDFERGRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKKN 120
           EEFVALNKFLLKLQQAFSD ERGRGYLVPDDVYEALVKIGF LDSPAFYTVCESFDQKKN
Sbjct: 63  EEFVALNKFLLKLQQAFSDLERGRGYLVPDDVYEALVKIGFMLDSPAFYTVCESFDQKKN 122

Query: 121 GRFRLDDFISLCIFVQSAG 140
           G+FRLDDFISLCIFVQSAG
Sbjct: 123 GKFRLDDFISLCIFVQSAG 141

[Close]

BLAST of Lsi10G008380 vs. NCBI nr
Match: XP_038900413.1 (sorcin-like isoform X3 [Benincasa hispida])

HSP 1 Score: 276.2 bits (705), Expect = 1.6e-70
Identity = 134/139 (96.40%), Postives = 135/139 (97.12%), Query Frame = 0


Query: 1   MENTGILKEWFNRVDSEKSGSITASQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF 60
           MENTGILKEWFNRVDSE SGSITA QLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF
Sbjct: 3   MENTGILKEWFNRVDSENSGSITAPQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF 62

Query: 61  EEFVALNKFLLKLQQAFSDFERGRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKKN 120
           EEFVALNKFLLKLQQAFSD ERGRGYLVPDDVYEALVKIGF LDSPAFYTVCESFDQKKN
Sbjct: 63  EEFVALNKFLLKLQQAFSDLERGRGYLVPDDVYEALVKIGFMLDSPAFYTVCESFDQKKN 122

Query: 121 GRFRLDDFISLCIFVQSAG 140
           G+FRLDDFISLCIFVQSAG
Sbjct: 123 GKFRLDDFISLCIFVQSAG 141

[Close]

BLAST of Lsi10G008380 vs. NCBI nr
Match: XP_016900564.1 (PREDICTED: sorcin-like [Cucumis melo])

HSP 1 Score: 275.4 bits (703), Expect = 2.7e-70
Identity = 134/139 (96.40%), Postives = 135/139 (97.12%), Query Frame = 0


Query: 1   MENTGILKEWFNRVDSEKSGSITASQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF 60
           MENTGILKEWF+RVDS  SGSITA QLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF
Sbjct: 1   MENTGILKEWFDRVDSANSGSITAPQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSF 60

Query: 61  EEFVALNKFLLKLQQAFSDFERGRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKKN 120
           EEFVALNKFLLKLQQAFSD ERGRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKKN
Sbjct: 61  EEFVALNKFLLKLQQAFSDLERGRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKKN 120

Query: 121 GRFRLDDFISLCIFVQSAG 140
           GRFRLDDFISLCIFVQSAG
Sbjct: 121 GRFRLDDFISLCIFVQSAG 139

[Close]

BLAST of Lsi10G008380 vs. TAIR 10
Match: AT5G04870.1 (calcium dependent protein kinase 1 )

HSP 1 Score: 73.6 bits (179), Expect = 1.4e-13
Identity = 39/132 (29.55%), Postives = 73/132 (55.30%), Query Frame = 0


Query: 7   LKEWFNRVDSEKSGSITASQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSFEEFVAL 66
           LKE FN +D++KSG IT  +L+  L     N   S +  +++  D D +GT+ ++EF+A 
Sbjct: 456 LKEMFNMIDADKSGQITFEELKAGLKRVGANLKESEILDLMQAADVDNSGTIDYKEFIAA 515

Query: 67  NKFLLKLQQ------AFSDFER-GRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKK 126
              L K+++      AF+ F++ G GY+ PD++ +A  + G  ++      +    DQ  
Sbjct: 516 TLHLNKIEREDHLFAAFTYFDKDGSGYITPDELQQACEEFG--VEDVRIEELMRDVDQDN 575

Query: 127 NGRFRLDDFISL 132
           +GR   ++F+++
Sbjct: 576 DGRIDYNEFVAM 585

[Close]

BLAST of Lsi10G008380 vs. TAIR 10
Match: AT3G10660.1 (calmodulin-domain protein kinase cdpk isoform 2 )

HSP 1 Score: 67.8 bits (164), Expect = 7.9e-12
Identity = 36/132 (27.27%), Postives = 71/132 (53.79%), Query Frame = 0


Query: 7   LKEWFNRVDSEKSGSITASQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSFEEFVAL 66
           LK+ F  +D++ SG IT  +L+  L     N   S +  +++  D D +GT+ ++EF+A 
Sbjct: 492 LKQMFKMIDADNSGQITFEELKAGLKRVGANLKESEILDLMQAADVDNSGTIDYKEFIAA 551

Query: 67  NKFLLKLQQ------AFSDFERGR-GYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKK 126
              L K+++      AFS F++   G++ PD++ +A  + G  ++      +    DQ K
Sbjct: 552 TLHLNKIEREDHLFAAFSYFDKDESGFITPDELQQACEEFG--VEDARIEEMMRDVDQDK 611

Query: 127 NGRFRLDDFISL 132
           +GR   ++F+++
Sbjct: 612 DGRIDYNEFVAM 621

[Close]

BLAST of Lsi10G008380 vs. TAIR 10
Match: AT5G12180.1 (calcium-dependent protein kinase 17 )

HSP 1 Score: 66.6 bits (161), Expect = 1.8e-11
Identity = 38/132 (28.79%), Postives = 66/132 (50.00%), Query Frame = 0


Query: 7   LKEWFNRVDSEKSGSITASQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSFEEFVAL 66
           LKE F  +D++ SG+IT  +L+  LA          VQQ++   D D NGT+ + EF+A 
Sbjct: 379 LKEMFKGMDTDSSGTITLEELRQGLAKQGTRLSEYEVQQLMEAADADGNGTIDYGEFIAA 438

Query: 67  NKFLLKLQQ------AFSDFER-GRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKK 126
              + +L +      AF  F++   GY+  +++ +AL + G   D      +    D   
Sbjct: 439 TMHINRLDREEHLYSAFQHFDKDNSGYITMEELEQALREFGMN-DGRDIKEIISEVDGDN 498

Query: 127 NGRFRLDDFISL 132
           +GR   D+F+++
Sbjct: 499 DGRINYDEFVAM 509

[Close]

BLAST of Lsi10G008380 vs. TAIR 10
Match: AT5G23580.1 (calmodulin-like domain protein kinase 9 )

HSP 1 Score: 66.2 bits (160), Expect = 2.3e-11
Identity = 38/137 (27.74%), Postives = 75/137 (54.74%), Query Frame = 0


Query: 2   ENTGILKEWFNRVDSEKSGSITASQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSFE 61
           E  G LKE F  +D++KSG+IT  +L++++         S +Q+++R  D D +GT+ + 
Sbjct: 323 EEIGGLKELFKMIDTDKSGTITFEELKDSMRRVGSELMESEIQELLRAADVDESGTIDYG 382

Query: 62  EFVALNKFLLKLQQ------AFSDFER-GRGYLVPDDVYEALVKIGFTLDSPAFYTVCES 121
           EF+A    L KL++      AFS F++   GY+  +++ +A  + G  ++      + + 
Sbjct: 383 EFLAATIHLNKLEREENLVAAFSFFDKDASGYITIEELQQAWKEFG--INDSNLDEMIKD 442

Query: 122 FDQKKNGRFRLDDFISL 132
            DQ  +G+    +F+++
Sbjct: 443 IDQDNDGQIDYGEFVAM 457

[Close]

BLAST of Lsi10G008380 vs. TAIR 10
Match: AT5G19360.1 (calcium-dependent protein kinase 34 )

HSP 1 Score: 65.5 bits (158), Expect = 3.9e-11
Identity = 37/132 (28.03%), Postives = 66/132 (50.00%), Query Frame = 0


Query: 7   LKEWFNRVDSEKSGSITASQLQNALAVGNLNFPHSIVQQMIRMYDFDRNGTMSFEEFVAL 66
           LKE F  +D++ SG+IT  +L+  LA          VQQ++   D D NGT+ + EF+A 
Sbjct: 374 LKEMFKGMDTDNSGTITLEELRQGLAKQGTRLSEYEVQQLMEAADADGNGTIDYGEFIAA 433

Query: 67  NKFLLKLQQ------AFSDFER-GRGYLVPDDVYEALVKIGFTLDSPAFYTVCESFDQKK 126
              + +L +      AF  F++   GY+  +++ +AL + G   D      +    D   
Sbjct: 434 TMHINRLDREEHLYSAFQHFDKDNSGYITTEELEQALREFGMN-DGRDIKEIISEVDGDN 493

Query: 127 NGRFRLDDFISL 132
           +GR   ++F+++
Sbjct: 494 DGRINYEEFVAM 504

The following BLAST results are available for this feature:

BLAST of Lsi10G008380 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94743	4.0e-16	36.96	Sorcin OS=Schistosoma japonicum OX=6182 PE=2 SV=1	[more]
Q06850	2.0e-12	29.55	Calcium-dependent protein kinase 1 OS=Arabidopsis thaliana OX=3702 GN=CPK1 PE=1 ...	[more]
Q38870	1.1e-10	27.27	Calcium-dependent protein kinase 2 OS=Arabidopsis thaliana OX=3702 GN=CPK2 PE=1 ...	[more]
Q2RAV0	1.1e-10	28.79	Calcium-dependent protein kinase 25 OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]
Q2QY37	1.1e-10	28.79	Calcium-dependent protein kinase 26 OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]

BLAST of Lsi10G008380 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KAX1	6.9e-72	97.84	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G118890 PE=4 SV=1	[more]
A0A1S4DX56	1.3e-70	96.40	sorcin-like OS=Cucumis melo OX=3656 GN=LOC103490457 PE=4 SV=1	[more]
A0A6J1JFX0	2.1e-68	94.93	sorcin-like OS=Cucurbita maxima OX=3661 GN=LOC111484048 PE=4 SV=1	[more]
A0A6J1CIP5	2.1e-68	93.53	sorcin-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011328 PE=4 SV=1	[more]
A0A6J1CGJ0	2.1e-68	93.53	sorcin-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011328 PE=4 SV=1	[more]

BLAST of Lsi10G008380 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004139969.1	1.4e-71	97.84	sorcin isoform X2 [Cucumis sativus] >KGN46663.1 hypothetical protein Csa_005521 ...	[more]
XP_011656916.1	1.4e-71	97.84	sorcin isoform X1 [Cucumis sativus]	[more]
XP_038900411.1	1.6e-70	96.40	sorcin-like isoform X1 [Benincasa hispida]	[more]
XP_038900413.1	1.6e-70	96.40	sorcin-like isoform X3 [Benincasa hispida]	[more]
XP_016900564.1	2.7e-70	96.40	PREDICTED: sorcin-like [Cucumis melo]	[more]

BLAST of Lsi10G008380 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G04870.1	1.4e-13	29.55	calcium dependent protein kinase 1	[more]
AT3G10660.1	7.9e-12	27.27	calmodulin-domain protein kinase cdpk isoform 2	[more]
AT5G12180.1	1.8e-11	28.79	calcium-dependent protein kinase 17	[more]
AT5G23580.1	2.3e-11	27.74	calmodulin-like domain protein kinase 9	[more]
AT5G19360.1	3.9e-11	28.03	calcium-dependent protein kinase 34	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 42..70 e-value: 0.011 score: 24.9 coord: 6..34 e-value: 11.0 score: 9.5
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 7..65 e-value: 7.2E-8 score: 32.8
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 2..37 score: 9.436442
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 38..73 score: 11.779655
None	No IPR available	GENE3D	1.10.238.10		coord: 1..138 e-value: 8.0E-33 score: 115.6
None	No IPR available	PANTHER	PTHR46212	PEFLIN	coord: 5..138
None	No IPR available	PANTHER	PTHR46212:SF3	PEFLIN	coord: 5..138
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 51..63
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 7..134

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G008380.1	Lsi10G008380.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding