Lsi10G008300 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi10G008300 vs. ExPASy Swiss-Prot
Match: P54797 (Transport and Golgi organization 2 homolog OS=Mus musculus OX=10090 GN=Tango2 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 5.1e-24
Identity = 87/320 (27.19%), Postives = 143/320 (44.69%), Query Frame = 0

Query: 1   MCIAAFLWKAHP----QYPLLLLLNRDEYRSRPTKPLEWW-ECGEILGGRD---GLAGGT 60
           MCI  F +   P     Y L+L  NRDE+ +RP+K  ++W    EIL G D   G AGGT
Sbjct: 1   MCIIFFKFDPRPVSKNAYRLILAANRDEFYNRPSKLADFWGNNSEILSGLDMEEGKAGGT 60

Query: 61  WLACSRNGRVAFITNFREVQSLPQAKSRGELPVRFLKIEDRNVWALARKILPMDFTELIN 120
           WL  S  G++  +TN+ + +  P A+ RGEL   FL                        
Sbjct: 61  WLGISTRGKLGALTNYLQPRQEPDARGRGELVSHFL------------------------ 120

Query: 121 MTMGIICHCHRSFFSSQKSPLDFAKEVAKEAHQYNGFNLIIADISSKTMSYITNRQTNAD 180
                         +S    L + K+V+ E H YNGFN+I AD+S+     +       +
Sbjct: 121 --------------TSDMDSLSYLKKVSTEGHLYNGFNIIAADLSTSKGDVVCYYGNRGE 180

Query: 181 SLVTEVSPGIHVLTNAQLDSPWPKAQRLDQSFRELINKNEDRELSAEEMVEKLMTNTVKD 240
                ++PG + L+NA L++PW K     Q F E + ++E   L  + +V +L+     +
Sbjct: 181 PEPIVLTPGTYGLSNALLETPWKKLCFGKQLFMEAVEQSE--ALPKDVLVTQLLDVLNNE 240

Query: 241 DVSLL-PGIYSPRTEY------QYSSIFVDTDTELGRFGTGSISVLSVKNSGEASFFERH 300
           +  L  P I     EY      +Y++++         +GT + +++ V  +G  +F ER 
Sbjct: 241 EAQLPDPAIEDQGQEYVQPILNKYAAVW----CRCASYGTRTNTIILVDANGHVTFTERS 276

Query: 301 L---ENDAWIDNHFTFQIEN 303
           +   +   W  N + F +++
Sbjct: 301 MLDKDTSRWETNTYEFTLQS 276

BLAST of Lsi10G008300 vs. ExPASy Swiss-Prot
Match: Q29RZ5 (Transport and Golgi organization protein 2 homolog OS=Bos taurus OX=9913 GN=TANGO2 PE=2 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.9e-23
Identity = 87/301 (28.90%), Postives = 134/301 (44.52%), Query Frame = 0

Query: 1   MCIAAFLWKAHP----QYPLLLLLNRDEYRSRPTKPLEWW-ECGEILGGRD---GLAGGT 60
           MCI  F +   P     Y L+L  NRDE+  RP +  ++W    E+L G D   G  GGT
Sbjct: 1   MCIIFFKFDPRPVSKNAYRLILAANRDEFYHRPARAADFWGSNNEVLSGLDMEEGKEGGT 60

Query: 61  WLACSRNGRVAFITNFREVQSLPQAKSRGELPVRFLKIEDRNVWALARKILPMDFTELIN 120
           WL  S  G++A +TN+ + +    A+ RGEL  +FL                        
Sbjct: 61  WLGISTRGKLAALTNYLQPRLNRNARGRGELVAQFL------------------------ 120

Query: 121 MTMGIICHCHRSFFSSQKSPLDFAKEVAKEAHQYNGFNLIIADISSKTMSYITNRQTNAD 180
                         +S    L + K+V+ E H YNGFNLI AD+S++    I       +
Sbjct: 121 --------------TSDMDSLSYLKKVSAEGHLYNGFNLIAADLSAEKGDVICYYGNRGE 180

Query: 181 SLVTEVSPGIHVLTNAQLDSPWPKAQRLDQSFRELINKNEDRELSAEEMVEKLMTNTVKD 240
                ++PG + L+NA L++PW K     Q F E + +   REL  + +V +L+     D
Sbjct: 181 REPVVLAPGTYGLSNALLETPWRKLCFGKQLFLEAVERG--RELPRDALVAQLLAVLSND 240

Query: 241 DVSLL-PGIYSPRTEY------QYSSIFVDTDTELGRFGTGSISVLSVKNSGEASFFERH 287
           +  L  P I +   EY      +Y+++ V        +GT + +V+ V   G  +F ER 
Sbjct: 241 EAQLPDPAIEAQGREYVRPILSKYAAVCV----RCPDYGTRTSTVILVDADGHVTFTERS 257

BLAST of Lsi10G008300 vs. ExPASy Swiss-Prot
Match: Q6ICL3 (Transport and Golgi organization protein 2 homolog OS=Homo sapiens OX=9606 GN=TANGO2 PE=2 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.8e-21
Identity = 86/301 (28.57%), Postives = 133/301 (44.19%), Query Frame = 0

Query: 1   MCIAAFLWKAHP----QYPLLLLLNRDEYRSRPTKPLEWW-ECGEILGGRD---GLAGGT 60
           MCI  F +   P     Y L+L  NRDE+ SRP+K  ++W    EIL G D   G  GGT
Sbjct: 1   MCIIFFKFDPRPVSKNAYRLILAANRDEFYSRPSKLADFWGNNNEILSGLDMEEGKEGGT 60

Query: 61  WLACSRNGRVAFITNFREVQSLPQAKSRGELPVRFLKIEDRNVWALARKILPMDFTELIN 120
           WL  S  G++A +TN+ + Q   QA+ RGEL   FL                        
Sbjct: 61  WLGISTRGKLAALTNYLQPQLDWQARGRGELVTHFL------------------------ 120

Query: 121 MTMGIICHCHRSFFSSQKSPLDFAKEVAKEAHQYNGFNLIIADISSKTMSYITNRQTNAD 180
                         ++    L + K+V+ E H YNGFNLI AD+S+     I       +
Sbjct: 121 --------------TTDVDSLSYLKKVSMEGHLYNGFNLIAADLSTAKGDVICYYGNRGE 180

Query: 181 SLVTEVSPGIHVLTNAQLDSPWPKAQRLDQSFRELINKNEDRELSAEEMVEKLMTNTVKD 240
                ++PG + L+NA L++PW K     Q F E + +++   L  + ++  L+     +
Sbjct: 181 PDPIVLTPGTYGLSNALLETPWRKLCFGKQLFLEAVERSQ--ALPKDVLIASLLDVLNNE 240

Query: 241 DVSLL-PGIYSPRTEY------QYSSIFVDTDTELGRFGTGSISVLSVKNSGEASFFERH 287
           +  L  P I     EY      +Y+++ V        +GT + +++ V   G  +F ER 
Sbjct: 241 EAQLPDPAIEDQGGEYVQPMLSKYAAVCV----RCPGYGTRTNTIILVDADGHVTFTERS 257

BLAST of Lsi10G008300 vs. ExPASy Swiss-Prot
Match: P53275 (Uncharacterized protein YGR127W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YGR127W PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 6.2e-06
Identity = 37/100 (37.00%), Postives = 51/100 (51.00%), Query Frame = 0

Query: 1  MCIAAFLWKAHPQYPLLLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAG--------GT 60
          MCI     +AHP Y L+L+ NRDE+ +R T    W     IL   D LA         GT
Sbjct: 1  MCI-LMATRAHPDYELILISNRDEFLARKTHATCWHNNDFILSPYD-LAKTSAEKQIFGT 60

Query: 61 WLACSRNGRVAFITNFR--EVQSLPQAKSRGELPVRFLKI 91
          W   ++ G++A I N +    Q+  +++SRG LP  FL I
Sbjct: 61 WSGINKEGKLATILNLKLDNEQNNTKSRSRGLLPFIFLSI 98

BLAST of Lsi10G008300 vs. ExPASy TrEMBL
Match: A0A6J1CII2 (transport and Golgi organization 2 homolog OS=Momordica charantia OX=3673 GN=LOC111011269 PE=4 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 1.2e-124
Identity = 230/303 (75.91%), Postives = 248/303 (81.85%), Query Frame = 0

Query: 1   MCIAAFLWKAHPQYPLLLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIAAFLW+AHP YP  LLLNRDEYR+RPTKPLEWWECG+ILGGRDGLAGGTWLACSRNG
Sbjct: 1   MCIAAFLWQAHPLYPFFLLLNRDEYRTRPTKPLEWWECGDILGGRDGLAGGTWLACSRNG 60

Query: 61  RVAFITNFREVQSLPQAKSRGELPVRFLKIEDRNVWALARKILPMDFTELINMTMGIICH 120
           RVAF+TNFREVQSLPQAKSRGELPVRFLK                               
Sbjct: 61  RVAFVTNFREVQSLPQAKSRGELPVRFLK------------------------------- 120

Query: 121 CHRSFFSSQKSPLDFAKEVAKEAHQYNGFNLIIADISSKTMSYITNRQTN-ADSLVTEVS 180
                  S+K+PLDFAKEVAKEA QYNGFNLI+A+ISSKTM YITNR TN + SLVTEVS
Sbjct: 121 -------SKKAPLDFAKEVAKEADQYNGFNLIVAEISSKTMFYITNRPTNDSPSLVTEVS 180

Query: 181 PGIHVLTNAQLDSPWPKAQRLDQSFRELINKNEDRELSAEEMVEKLMTNTVKDDVSLLPG 240
           PGIHVLTNAQLDSPWPKAQRLDQSFR+L++KN DREL  EEMVEKLMTNT+KDD SLLPG
Sbjct: 181 PGIHVLTNAQLDSPWPKAQRLDQSFRDLVDKNGDRELPTEEMVEKLMTNTIKDDESLLPG 240

Query: 241 IYSPRTEYQYSSIFVDTDTELGRFGTGSISVLSVKNSGEASFFERHLENDAWIDNHFTFQ 300
           IYSPR EYQYSSIFVDTDTELGR+GTGSIS LSVKNSGEA+F +R+L+ND WIDN FTFQ
Sbjct: 241 IYSPRREYQYSSIFVDTDTELGRYGTGSISALSVKNSGEATFSQRNLQNDEWIDNSFTFQ 265

Query: 301 IEN 303
           IEN
Sbjct: 301 IEN 265

BLAST of Lsi10G008300 vs. ExPASy TrEMBL
Match: A0A6J1I2B1 (transport and Golgi organization protein 2 homolog OS=Cucurbita maxima OX=3661 GN=LOC111469854 PE=4 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 6.4e-123
Identity = 228/303 (75.25%), Postives = 242/303 (79.87%), Query Frame = 0

Query: 1   MCIAAFLWKAHPQYPLLLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIAAFLW+AHP YP  LLLNRDEY SRPTKPLEWWEC EILGGRDGLAGGTWLACSRNG
Sbjct: 1   MCIAAFLWQAHPLYPFFLLLNRDEYHSRPTKPLEWWECNEILGGRDGLAGGTWLACSRNG 60

Query: 61  RVAFITNFREVQSLPQAKSRGELPVRFLKIEDRNVWALARKILPMDFTELINMTMGIICH 120
           RVAF+TNFREV+ LPQAKSRGELPVRFLK                               
Sbjct: 61  RVAFLTNFREVELLPQAKSRGELPVRFLK------------------------------- 120

Query: 121 CHRSFFSSQKSPLDFAKEVAKEAHQYNGFNLIIADISSKTMSYITNRQ-TNADSLVTEVS 180
                  S+K+PLDFAKEV KEAHQ+NGFNLIIADI SKTM YITNR   N+DSLVTEV 
Sbjct: 121 -------SKKTPLDFAKEVEKEAHQFNGFNLIIADIPSKTMFYITNRPINNSDSLVTEVP 180

Query: 181 PGIHVLTNAQLDSPWPKAQRLDQSFRELINKNEDRELSAEEMVEKLMTNTVKDDVSLLPG 240
           PGIHVLTNAQLDSPWPKA RLDQS RE +NKN DREL  +EMVEKLMTNT+KDDVSLLPG
Sbjct: 181 PGIHVLTNAQLDSPWPKALRLDQSLREFVNKNGDRELPVKEMVEKLMTNTIKDDVSLLPG 240

Query: 241 IYSPRTEYQYSSIFVDTDTELGRFGTGSISVLSVKNSGEASFFERHLENDAWIDNHFTFQ 300
           IYSPR EYQYSSIFVDTD+ELGR+GTGSIS LSVKNSGEASFFE+HLENDAWID+  TFQ
Sbjct: 241 IYSPRREYQYSSIFVDTDSELGRYGTGSISALSVKNSGEASFFEKHLENDAWIDSSVTFQ 265

Query: 301 IEN 303
           IEN
Sbjct: 301 IEN 265

BLAST of Lsi10G008300 vs. ExPASy TrEMBL
Match: A0A6J1G6B2 (transport and Golgi organization protein 2 homolog OS=Cucurbita moschata OX=3662 GN=LOC111451268 PE=4 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 2.4e-122
Identity = 227/303 (74.92%), Postives = 240/303 (79.21%), Query Frame = 0

Query: 1   MCIAAFLWKAHPQYPLLLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIAAFLW+AHP YP  LLLNRDEY SRPTKPLEWWEC EILGGRDGLAGGTWLACSRNG
Sbjct: 1   MCIAAFLWQAHPLYPFFLLLNRDEYHSRPTKPLEWWECNEILGGRDGLAGGTWLACSRNG 60

Query: 61  RVAFITNFREVQSLPQAKSRGELPVRFLKIEDRNVWALARKILPMDFTELINMTMGIICH 120
           RVAF+TNFREV+ LPQAKSRGELPVRFLK                               
Sbjct: 61  RVAFLTNFREVELLPQAKSRGELPVRFLK------------------------------- 120

Query: 121 CHRSFFSSQKSPLDFAKEVAKEAHQYNGFNLIIADISSKTMSYITNRQ-TNADSLVTEVS 180
                  S+ +PLDFAKEV KEAHQYNGFNLII DI SKTM YITNR   N+DSLVTEV 
Sbjct: 121 -------SKTTPLDFAKEVEKEAHQYNGFNLIIVDIPSKTMFYITNRPINNSDSLVTEVP 180

Query: 181 PGIHVLTNAQLDSPWPKAQRLDQSFRELINKNEDRELSAEEMVEKLMTNTVKDDVSLLPG 240
           PGIHVLTNAQLDSPWPKA RLDQS RE +NKN DREL  +EMVEKLM NT+KDDVSLLPG
Sbjct: 181 PGIHVLTNAQLDSPWPKALRLDQSLREFVNKNGDRELPVKEMVEKLMINTIKDDVSLLPG 240

Query: 241 IYSPRTEYQYSSIFVDTDTELGRFGTGSISVLSVKNSGEASFFERHLENDAWIDNHFTFQ 300
           IYSPR EYQYSSIFVDTD+ELGR+GTGSIS LSVKNSGEASFFE+HLENDAWID+ FTFQ
Sbjct: 241 IYSPRREYQYSSIFVDTDSELGRYGTGSISALSVKNSGEASFFEKHLENDAWIDSSFTFQ 265

Query: 301 IEN 303
           IEN
Sbjct: 301 IEN 265

BLAST of Lsi10G008300 vs. ExPASy TrEMBL
Match: A0A2N9FED1 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS13380 PE=4 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 4.8e-94
Identity = 180/301 (59.80%), Postives = 211/301 (70.10%), Query Frame = 0

Query: 1   MCIAAFLWKAHPQYPLLLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIA FLW+AHP YPL+LLLNRDEY SRPT+ LEWWE  EILGGRD LAGGTWLACSR+G
Sbjct: 66  MCIAVFLWQAHPLYPLILLLNRDEYHSRPTRQLEWWEGDEILGGRDELAGGTWLACSRDG 125

Query: 61  RVAFITNFREVQSLPQAKSRGELPVRFLKIEDRNVWALARKILPMDFTELINMTMGIICH 120
           RVAF+TN REVQSLP+AKSRG+LPVRFL+                               
Sbjct: 126 RVAFLTNVREVQSLPEAKSRGDLPVRFLQ------------------------------- 185

Query: 121 CHRSFFSSQKSPLDFAKEVAKEAHQYNGFNLIIADISSKTMSYITNRQTNADSLVTEVSP 180
                  S+K P++FA+EVAKE  QYNGFNLI+ D+  KTM Y+TNR    +  VTEVS 
Sbjct: 186 -------SKKKPMEFAEEVAKEMDQYNGFNLILIDLCCKTMVYVTNRAKEDNKFVTEVST 245

Query: 181 GIHVLTNAQLDSPWPKAQRLDQSFRELINKNEDRELSAEEMVEKLMTNTVKDDVSLLPGI 240
           GIHVLTNA LDSPWPKAQRL  SF+EL+ K  + EL  +EMVEKLMTNTVKDD S+LP I
Sbjct: 246 GIHVLTNASLDSPWPKAQRLGNSFKELLEKYGEEELPMKEMVEKLMTNTVKDDESMLPHI 305

Query: 241 YSPRTEYQYSSIFVDTDTELGRFGTGSISVLSVKNSGEASFFERHLENDAWIDNHFTFQI 300
           + P  EY  SSIFVDT++ LG +GT S S L VK +GE  F+ERHLEN+ W +   T+QI
Sbjct: 306 FPPEREYHLSSIFVDTNSPLGPYGTRSTSALFVKATGEVGFYERHLENELWKEQAVTYQI 328

Query: 301 E 302
           +
Sbjct: 366 K 328

BLAST of Lsi10G008300 vs. ExPASy TrEMBL
Match: A0A6A1UHJ9 (Uncharacterized protein OS=Morella rubra OX=262757 GN=CJ030_MR0G025146 PE=4 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 1.1e-93
Identity = 174/302 (57.62%), Postives = 213/302 (70.53%), Query Frame = 0

Query: 1   MCIAAFLWKAHPQYPLLLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIA FLW+AHP YPL+LL NRDEY SRPT+PL WWE G++L GRD LAGGTWLACSR+G
Sbjct: 1   MCIAVFLWQAHPLYPLILLQNRDEYHSRPTRPLAWWEGGQVLAGRDELAGGTWLACSRDG 60

Query: 61  RVAFITNFREVQSLPQAKSRGELPVRFLKIEDRNVWALARKILPMDFTELINMTMGIICH 120
           RVAF+TNFREV+ LP+AKSRG+LPVRFL+                               
Sbjct: 61  RVAFLTNFREVKKLPEAKSRGDLPVRFLQ------------------------------- 120

Query: 121 CHRSFFSSQKSPLDFAKEVAKEAHQYNGFNLIIADISSKTMSYITNRQTNADSLVTEVSP 180
                  S+K P +FA+EV KEA QYNGFNLI+ DI SKTM Y+TNR    D  VT+V P
Sbjct: 121 -------SKKKPAEFAEEVVKEADQYNGFNLILTDIGSKTMVYVTNRAKEGDCSVTKVEP 180

Query: 181 GIHVLTNAQLDSPWPKAQRLDQSFRELINKNEDRELSAEEMVEKLMTNTVKDDVSLLPGI 240
           G HVLTNA L+SPWPKA+RL  +F+EL++KN ++E+  +EMVEKLM NTVKDD S+LP I
Sbjct: 181 GTHVLTNATLNSPWPKAERLGTNFKELLSKNGEKEVPMKEMVEKLMMNTVKDDESMLPHI 240

Query: 241 YSPRTEYQYSSIFVDTDTELGRFGTGSISVLSVKNSGEASFFERHLENDAWIDNHFTFQI 300
           + P  EY  SS+FVD+D+ LGR+GT SIS L VK +G   F+ERHLEN+ W +   T+QI
Sbjct: 241 HPPEWEYPLSSVFVDSDSPLGRYGTRSISALFVKTTGGVDFYERHLENELWKEQTLTYQI 264

Query: 301 EN 303
           E+
Sbjct: 301 ED 264

BLAST of Lsi10G008300 vs. NCBI nr
Match: XP_038879622.1 (transport and Golgi organization 2 homolog [Benincasa hispida])

HSP 1 Score: 491.5 bits (1264), Expect = 5.2e-135
Identity = 253/304 (83.22%), Postives = 258/304 (84.87%), Query Frame = 0

Query: 1   MCIAAFLWKAHPQYPLLLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIAAFLW+AHP Y L+LLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG
Sbjct: 1   MCIAAFLWQAHPLYSLVLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60

Query: 61  RVAFITNFREVQSLPQAKSRGELPVRFLKIEDRNVWALARKILPMDFTELINMTMGIICH 120
           RVAFITNFREVQSLPQAKSRGELPVRFLK                               
Sbjct: 61  RVAFITNFREVQSLPQAKSRGELPVRFLK------------------------------- 120

Query: 121 CHRSFFSSQKSPLDFAKEVAKEAHQYNGFNLIIADISSKTMSYITNRQTN-ADSLVTEVS 180
                  SQKSPLDFAKEVAK AH YNGFNLIIADISSKTMSYITNRQTN +DSLVTEVS
Sbjct: 121 -------SQKSPLDFAKEVAKGAHLYNGFNLIIADISSKTMSYITNRQTNDSDSLVTEVS 180

Query: 181 PGIHVLTNAQLDSPWPKAQRLDQSFRELINKNEDRELSAEEMVEKLMTNTVKDDVSLLPG 240
           PGIH+LTNAQLDSPWPKAQRLDQSFRELINKN D ELSAEEMVEK+MTNTVKDDVSLLPG
Sbjct: 181 PGIHILTNAQLDSPWPKAQRLDQSFRELINKNGDGELSAEEMVEKIMTNTVKDDVSLLPG 240

Query: 241 IYSPRTEYQYSSIFVDTDTELGRFGTGSISVLSVKNSGEASFFERHLENDAWIDNHFTFQ 300
           IYSPRTEYQYSSIFVDTDTELGRFGT SISVLSVKNSGEASFFERHLENDAWIDNHFTFQ
Sbjct: 241 IYSPRTEYQYSSIFVDTDTELGRFGTRSISVLSVKNSGEASFFERHLENDAWIDNHFTFQ 266

Query: 301 IENV 304
           IENV
Sbjct: 301 IENV 266

BLAST of Lsi10G008300 vs. NCBI nr
Match: XP_022140668.1 (transport and Golgi organization 2 homolog [Momordica charantia])

HSP 1 Score: 456.1 bits (1172), Expect = 2.4e-124
Identity = 230/303 (75.91%), Postives = 248/303 (81.85%), Query Frame = 0

Query: 1   MCIAAFLWKAHPQYPLLLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIAAFLW+AHP YP  LLLNRDEYR+RPTKPLEWWECG+ILGGRDGLAGGTWLACSRNG
Sbjct: 1   MCIAAFLWQAHPLYPFFLLLNRDEYRTRPTKPLEWWECGDILGGRDGLAGGTWLACSRNG 60

Query: 61  RVAFITNFREVQSLPQAKSRGELPVRFLKIEDRNVWALARKILPMDFTELINMTMGIICH 120
           RVAF+TNFREVQSLPQAKSRGELPVRFLK                               
Sbjct: 61  RVAFVTNFREVQSLPQAKSRGELPVRFLK------------------------------- 120

Query: 121 CHRSFFSSQKSPLDFAKEVAKEAHQYNGFNLIIADISSKTMSYITNRQTN-ADSLVTEVS 180
                  S+K+PLDFAKEVAKEA QYNGFNLI+A+ISSKTM YITNR TN + SLVTEVS
Sbjct: 121 -------SKKAPLDFAKEVAKEADQYNGFNLIVAEISSKTMFYITNRPTNDSPSLVTEVS 180

Query: 181 PGIHVLTNAQLDSPWPKAQRLDQSFRELINKNEDRELSAEEMVEKLMTNTVKDDVSLLPG 240
           PGIHVLTNAQLDSPWPKAQRLDQSFR+L++KN DREL  EEMVEKLMTNT+KDD SLLPG
Sbjct: 181 PGIHVLTNAQLDSPWPKAQRLDQSFRDLVDKNGDRELPTEEMVEKLMTNTIKDDESLLPG 240

Query: 241 IYSPRTEYQYSSIFVDTDTELGRFGTGSISVLSVKNSGEASFFERHLENDAWIDNHFTFQ 300
           IYSPR EYQYSSIFVDTDTELGR+GTGSIS LSVKNSGEA+F +R+L+ND WIDN FTFQ
Sbjct: 241 IYSPRREYQYSSIFVDTDTELGRYGTGSISALSVKNSGEATFSQRNLQNDEWIDNSFTFQ 265

Query: 301 IEN 303
           IEN
Sbjct: 301 IEN 265

BLAST of Lsi10G008300 vs. NCBI nr
Match: KAG6604975.1 (Transport and Golgi organization protein 2-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 452.2 bits (1162), Expect = 3.5e-123
Identity = 229/303 (75.58%), Postives = 242/303 (79.87%), Query Frame = 0

Query: 1   MCIAAFLWKAHPQYPLLLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIAAFLW+AHP YP  LLLNRDEY SRPTKPLEWWEC EILGGRDGLAGGTWLACSRNG
Sbjct: 56  MCIAAFLWQAHPLYPFFLLLNRDEYHSRPTKPLEWWECNEILGGRDGLAGGTWLACSRNG 115

Query: 61  RVAFITNFREVQSLPQAKSRGELPVRFLKIEDRNVWALARKILPMDFTELINMTMGIICH 120
           RVAF+TNFREV+ LPQAKSRGELPVRFLK                               
Sbjct: 116 RVAFLTNFREVELLPQAKSRGELPVRFLK------------------------------- 175

Query: 121 CHRSFFSSQKSPLDFAKEVAKEAHQYNGFNLIIADISSKTMSYITNRQ-TNADSLVTEVS 180
                  S+ +PLDFAKEV KEAHQYNGFNLIIADI SKTM YITNR   N+DSLVTEV 
Sbjct: 176 -------SKTTPLDFAKEVEKEAHQYNGFNLIIADIPSKTMFYITNRPINNSDSLVTEVP 235

Query: 181 PGIHVLTNAQLDSPWPKAQRLDQSFRELINKNEDRELSAEEMVEKLMTNTVKDDVSLLPG 240
           PGIHVLTNAQLDSPWPKA RLDQS RE +NKN DREL  +EMVEKLMTNT+KDDVSLLPG
Sbjct: 236 PGIHVLTNAQLDSPWPKALRLDQSLREFVNKNGDRELPVKEMVEKLMTNTIKDDVSLLPG 295

Query: 241 IYSPRTEYQYSSIFVDTDTELGRFGTGSISVLSVKNSGEASFFERHLENDAWIDNHFTFQ 300
           IYSPR EYQYSSIFVDTD+ELGR+GTGSIS LSVKNSGEASFFE+HLENDAWID+ FTFQ
Sbjct: 296 IYSPRREYQYSSIFVDTDSELGRYGTGSISALSVKNSGEASFFEKHLENDAWIDSSFTFQ 320

Query: 301 IEN 303
           IEN
Sbjct: 356 IEN 320

BLAST of Lsi10G008300 vs. NCBI nr
Match: XP_022971076.1 (transport and Golgi organization protein 2 homolog [Cucurbita maxima] >XP_022971077.1 transport and Golgi organization protein 2 homolog [Cucurbita maxima])

HSP 1 Score: 450.3 bits (1157), Expect = 1.3e-122
Identity = 228/303 (75.25%), Postives = 242/303 (79.87%), Query Frame = 0

Query: 1   MCIAAFLWKAHPQYPLLLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIAAFLW+AHP YP  LLLNRDEY SRPTKPLEWWEC EILGGRDGLAGGTWLACSRNG
Sbjct: 1   MCIAAFLWQAHPLYPFFLLLNRDEYHSRPTKPLEWWECNEILGGRDGLAGGTWLACSRNG 60

Query: 61  RVAFITNFREVQSLPQAKSRGELPVRFLKIEDRNVWALARKILPMDFTELINMTMGIICH 120
           RVAF+TNFREV+ LPQAKSRGELPVRFLK                               
Sbjct: 61  RVAFLTNFREVELLPQAKSRGELPVRFLK------------------------------- 120

Query: 121 CHRSFFSSQKSPLDFAKEVAKEAHQYNGFNLIIADISSKTMSYITNRQ-TNADSLVTEVS 180
                  S+K+PLDFAKEV KEAHQ+NGFNLIIADI SKTM YITNR   N+DSLVTEV 
Sbjct: 121 -------SKKTPLDFAKEVEKEAHQFNGFNLIIADIPSKTMFYITNRPINNSDSLVTEVP 180

Query: 181 PGIHVLTNAQLDSPWPKAQRLDQSFRELINKNEDRELSAEEMVEKLMTNTVKDDVSLLPG 240
           PGIHVLTNAQLDSPWPKA RLDQS RE +NKN DREL  +EMVEKLMTNT+KDDVSLLPG
Sbjct: 181 PGIHVLTNAQLDSPWPKALRLDQSLREFVNKNGDRELPVKEMVEKLMTNTIKDDVSLLPG 240

Query: 241 IYSPRTEYQYSSIFVDTDTELGRFGTGSISVLSVKNSGEASFFERHLENDAWIDNHFTFQ 300
           IYSPR EYQYSSIFVDTD+ELGR+GTGSIS LSVKNSGEASFFE+HLENDAWID+  TFQ
Sbjct: 241 IYSPRREYQYSSIFVDTDSELGRYGTGSISALSVKNSGEASFFEKHLENDAWIDSSVTFQ 265

Query: 301 IEN 303
           IEN
Sbjct: 301 IEN 265

BLAST of Lsi10G008300 vs. NCBI nr
Match: XP_022947387.1 (transport and Golgi organization protein 2 homolog [Cucurbita moschata] >XP_022947388.1 transport and Golgi organization protein 2 homolog [Cucurbita moschata])

HSP 1 Score: 448.4 bits (1152), Expect = 5.1e-122
Identity = 227/303 (74.92%), Postives = 240/303 (79.21%), Query Frame = 0

Query: 1   MCIAAFLWKAHPQYPLLLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIAAFLW+AHP YP  LLLNRDEY SRPTKPLEWWEC EILGGRDGLAGGTWLACSRNG
Sbjct: 1   MCIAAFLWQAHPLYPFFLLLNRDEYHSRPTKPLEWWECNEILGGRDGLAGGTWLACSRNG 60

Query: 61  RVAFITNFREVQSLPQAKSRGELPVRFLKIEDRNVWALARKILPMDFTELINMTMGIICH 120
           RVAF+TNFREV+ LPQAKSRGELPVRFLK                               
Sbjct: 61  RVAFLTNFREVELLPQAKSRGELPVRFLK------------------------------- 120

Query: 121 CHRSFFSSQKSPLDFAKEVAKEAHQYNGFNLIIADISSKTMSYITNRQ-TNADSLVTEVS 180
                  S+ +PLDFAKEV KEAHQYNGFNLII DI SKTM YITNR   N+DSLVTEV 
Sbjct: 121 -------SKTTPLDFAKEVEKEAHQYNGFNLIIVDIPSKTMFYITNRPINNSDSLVTEVP 180

Query: 181 PGIHVLTNAQLDSPWPKAQRLDQSFRELINKNEDRELSAEEMVEKLMTNTVKDDVSLLPG 240
           PGIHVLTNAQLDSPWPKA RLDQS RE +NKN DREL  +EMVEKLM NT+KDDVSLLPG
Sbjct: 181 PGIHVLTNAQLDSPWPKALRLDQSLREFVNKNGDRELPVKEMVEKLMINTIKDDVSLLPG 240

Query: 241 IYSPRTEYQYSSIFVDTDTELGRFGTGSISVLSVKNSGEASFFERHLENDAWIDNHFTFQ 300
           IYSPR EYQYSSIFVDTD+ELGR+GTGSIS LSVKNSGEASFFE+HLENDAWID+ FTFQ
Sbjct: 241 IYSPRREYQYSSIFVDTDSELGRYGTGSISALSVKNSGEASFFEKHLENDAWIDSSFTFQ 265

Query: 301 IEN 303
           IEN
Sbjct: 301 IEN 265

BLAST of Lsi10G008300 vs. TAIR 10
Match: AT4G38260.1 (Protein of unknown function (DUF833) )

HSP 1 Score: 291.2 bits (744), Expect = 9.6e-79
Identity = 149/307 (48.53%), Postives = 195/307 (63.52%), Query Frame = 0

Query: 1   MCIAAFLWKAHPQYPLLLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           MCIA FLW++HP YP LL LNRDE  +R T+ L WWE GE +GGRD + GGTWL C+R+G
Sbjct: 1   MCIAVFLWQSHPLYPFLLFLNRDEDHNRATEALRWWEDGETVGGRDLVGGGTWLGCTRHG 60

Query: 61  RVAFITNFREVQSLPQAKSRGELPVRFLKIEDRNVWALARKILPMDFTELINMTMGIICH 120
           R+AF+TNF+E  S P AKSRG+LP+R+L+                               
Sbjct: 61  RLAFLTNFKEASSFPAAKSRGDLPLRYLQ------------------------------- 120

Query: 121 CHRSFFSSQKSPLDFAKEVAKEAHQYNGFNLIIADISSKTMSYITNRQTNADSLVTEVSP 180
                  S+KSP +FA+E+  E   YNGFNL++A + SK+M YITNR  + D LVT+VSP
Sbjct: 121 -------SEKSPAEFAEEIQDEISLYNGFNLVVAHVLSKSMIYITNRPPHGDKLVTQVSP 180

Query: 181 GIHVLTNAQLDSPWPKAQRLDQSFRELINKNEDRELSAEEMVEKLMTNTVKDDVSLLPGI 240
           GIHVL+NA LDSPWPK  RL + F++L+ +N   E   + MVE++MTNTVKD+ + LP +
Sbjct: 181 GIHVLSNANLDSPWPKCLRLREGFQQLLAENGSGEFPVKTMVEEVMTNTVKDEETELPHV 240

Query: 241 YSPRTEYQYSSIFVDTDTELGRFGTGSISVLSVKN------SGEASFFERHL-ENDAWID 300
           ++P TEY  SSIFVD     GR+GT SIS + VK+       GE  F+ERHL E D+W +
Sbjct: 241 FTPETEYHLSSIFVDMQRPTGRYGTRSISAIIVKSHGDGGGDGEICFYERHLEEGDSWKE 269

BLAST of Lsi10G008300 vs. TAIR 10
Match: AT1G20730.1 (Protein of unknown function (DUF833) )

HSP 1 Score: 96.7 bits (239), Expect = 3.5e-20
Identity = 99/309 (32.04%), Postives = 134/309 (43.37%), Query Frame = 0

Query: 1   MCIAAFLWKAHPQYPLLLLLNRDEYRSRPTKPLEW-WECGEILGGRDGLAGGTWLACSRN 60
           M I AF W    +  L LL NR+ ++SR      W W+  ++L GR     GTWL  S  
Sbjct: 1   MGIVAFQW-GEGENILTLLQNRENWQSRSIGKAAWEWDESQVLSGRCAETDGTWLGISIR 60

Query: 61  GRVAFITNFREVQSLPQAKSRGELPVRFLKIEDRNVWALARKILPMDFTELINMTMGIIC 120
           GRVAF+     V                    DR + A  R +                 
Sbjct: 61  GRVAFLVEAGPVN------------------RDRIIGAECRTL----------------- 120

Query: 121 HCHRSFFSSQKSPLDFAKEVAKEAHQYNG--FNLIIADISSKTMSYITNRQTNADSLV-T 180
                F  S  SP DFA  +A ++ +  G  ++LI+ADI S +M YI     + D +V T
Sbjct: 121 ----DFLKSNLSPEDFADSLALDSGRNTGIAYHLIVADIVSNSMFYIYKPSLSEDGMVYT 180

Query: 181 E-VSPGIHVLTNAQLDSP-WPKAQRLDQSFRELINKNEDRELSAEEMVEKLMTNTVKDDV 240
           E V PG+H L++A LDS    +  RL + F E IN    REL   E +  L    + D V
Sbjct: 181 EPVGPGVHTLSSAGLDSEVGHRDLRLKRYFSERIN----REL--PEPISGLAEEVMYDTV 240

Query: 241 SLLPGIYSPRTEYQYSSIF-VDTDTELGRFGTGSISVLSVKNSGEASFFERHLE--NDAW 300
             + G          SSIF VDT  E   +GT   + L V+ + +  FFER+    ND W
Sbjct: 241 EAING-------DPLSSIFVVDTLIENEHYGTRCTTALVVRRTMQVRFFERYRARFNDDW 256

BLAST of Lsi10G008300 vs. TAIR 10
Match: AT1G20680.2 (Protein of unknown function (DUF833) )

HSP 1 Score: 96.3 bits (238), Expect = 4.5e-20
Identity = 86/310 (27.74%), Postives = 136/310 (43.87%), Query Frame = 0

Query: 1   MCIAAFLWKAHPQYPLLLLLNRDEYRSRPTKPLEWWECGEILGGRDGLAGGTWLACSRNG 60
           M I AF W       L LL+NRD + +R      W   G+IL GR     GTW   ++ G
Sbjct: 1   MGIVAFEWAEGKTNQLTLLMNRDNWENRVISGASWSRNGQILSGRCKANNGTWFGITKGG 60

Query: 61  RVAFITNFREVQSLPQAKSRGEL-PVRFLKIEDRNVWALARKILPMDFTELINMTMGIIC 120
           RVAF+ N   +    ++ S  EL PVRFL+                              
Sbjct: 61  RVAFLVNTSLLLDRVKSYSGSELYPVRFLE------------------------------ 120

Query: 121 HCHRSFFSSQKSPLDFAKEV---AKEAHQYNGFNLIIADISSKTMSYITN-RQTNADSLV 180
                      SP  FA EV    KE ++ + ++L++AD++S +M +I     T +D ++
Sbjct: 121 --------GNMSPEQFANEVKVHEKETNERHAYSLVVADMTSSSMVHILKPSDTKSDVVI 180

Query: 181 TEVSPGIHVLTNAQ-LDS-PWPKAQRLDQSFRELI-NKNEDRELSAEEMVEKLMTNTVKD 240
             V  G+H L++ + LDS    +   L + F +++ N    ++   EE+  + M +    
Sbjct: 181 ETVPFGVHTLSSYEGLDSTDSARDLLLRRLFTQMVGNLGNVQQRQMEEIAGRFMYDAQAG 240

Query: 241 DVSLLPGIYSPRTEYQYSSIFVDTDTELGRFGTGSISVLSVKNSGEASFFERHLE-NDAW 300
             ++    Y  R E+    +         RFGT S + L VK + E   FE+++E N AW
Sbjct: 241 RDAV---FYHSRDEHPNGKLGTQ------RFGTTSTTALVVKRTREVMLFEKYMEQNGAW 263

Query: 301 IDNHFTFQIE 302
             N+F F I+
Sbjct: 301 NTNNFAFNIQ 263

BLAST of Lsi10G008300 vs. TAIR 10
Match: AT1G20740.1 (Protein of unknown function (DUF833) )

HSP 1 Score: 84.7 bits (208), Expect = 1.4e-16
Identity = 87/283 (30.74%), Postives = 120/283 (42.40%), Query Frame = 0

Query: 28  RPTKPLEW--WECGEILGGRDGLAGGTWLACSRNGRVAFITNFREVQSLPQAKSRGELPV 87
           R  +  EW   E  +IL GR     GTWL  S  GRVAF+     +              
Sbjct: 35  RSIRKAEWVKTETDQILSGRCPETDGTWLGISTRGRVAFLVEAGTINR-----------D 94

Query: 88  RFLKIEDRNVWALARKILPMDFTELINMTMGIICHCHRSFFSSQKSPLDFAKEVAKEAHQ 147
           RF   E R +                             F  S +SP DFAK  A +  +
Sbjct: 95  RFNGAESRTL----------------------------EFLESNESPEDFAKSSAADYIR 154

Query: 148 YN---GFNLIIADISSKTMSYITN-RQTNADSLVTE-VSPGIHVLTNAQLDSP-WPKAQR 207
                 F+LI+ADI+S +M YI+  R ++   + TE V PG+H L++A LDS    +  R
Sbjct: 155 NKNTAAFHLIVADIASNSMLYISKPRFSDYGIVYTEPVGPGVHTLSSAGLDSDVGYRDLR 214

Query: 208 LDQSFRELINKNEDRELSAEEMVEKLMTNTVKDDVSLLPGIYSPRTEYQYSSIFVDTDTE 267
           +  SF E+IN+     L     + ++M + VK   S+L            S  FVD    
Sbjct: 215 MRHSFCEMINR---ERLPPIRDIAEIMYDPVKAYESVLLS----------SIFFVDMKIG 265

Query: 268 LGRFGTGSISVLSVKNSGEASFFERHLE--NDAWIDNHFTFQI 301
              +GT   + L VK + E  FFER+ E  ND W D+ F F I
Sbjct: 275 YEHYGTRITTALVVKRTKEVLFFERYREIFNDDWDDHDFAFTI 265

BLAST of Lsi10G008300 vs. TAIR 10
Match: AT1G20680.1 (Protein of unknown function (DUF833) )

HSP 1 Score: 70.9 bits (172), Expect = 2.0e-12
Identity = 69/275 (25.09%), Postives = 116/275 (42.18%), Query Frame = 0

Query: 35  WWECGEILGGRDGLAGGTWLACSRNGRVAFITNFREVQSLPQAKSRGELPVRFLKIEDRN 94
           W   G+IL GR     GTW   ++ GRVAF+ N   +    ++ S  EL           
Sbjct: 43  WSRNGQILSGRCKANNGTWFGITKGGRVAFLVNTSLLLDRVKSYSGSEL----------- 102

Query: 95  VWALARKILPMDFTELINMTMGIICHCHRSFFSSQKSPLDFAKEV---AKEAHQYNGFNL 154
                                          +    SP  FA EV    KE ++ + ++L
Sbjct: 103 -------------------------------YPGNMSPEQFANEVKVHEKETNERHAYSL 162

Query: 155 IIADISSKTMSYITN-RQTNADSLVTEVSPGIHVLTNAQ-LDS-PWPKAQRLDQSFRELI 214
           ++AD++S +M +I     T +D ++  V  G+H L++ + LDS    +   L + F +++
Sbjct: 163 VVADMTSSSMVHILKPSDTKSDVVIETVPFGVHTLSSYEGLDSTDSARDLLLRRLFTQMV 222

Query: 215 -NKNEDRELSAEEMVEKLMTNTVKDDVSLLPGIYSPRTEYQYSSIFVDTDTELGRFGTGS 274
            N    ++   EE+  + M +      ++    Y  R E+    +         RFGT S
Sbjct: 223 GNLGNVQQRQMEEIAGRFMYDAQAGRDAV---FYHSRDEHPNGKLGTQ------RFGTTS 266

Query: 275 ISVLSVKNSGEASFFERHLE-NDAWIDNHFTFQIE 302
            + L VK + E   FE+++E N AW  N+F F I+
Sbjct: 283 TTALVVKRTREVMLFEKYMEQNGAWNTNNFAFNIQ 266