Lsi10G008050 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G008050
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionreceptor-like cytosolic serine/threonine-protein kinase RBK1
Locationchr10: 10984090 .. 10986269 (-)
RNA-Seq ExpressionLsi10G008050
SyntenyLsi10G008050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGTCCTTCATCAGGCCCAAGACTCAGAAGAAGAGAACAATTATCTTTGGCCTAAAATCTGACAATGGAAGCAGAGATATTCTCCTTTGGTTGCTTACGACGATTGTCAATCCGGGAGATTATGTTCTGGCTATTCATGTTCAGGAGCCAAATGACAGTTTCAATCCAAATACTTTCCACATCCATGAAGATATTTGCAAATCCAAACAGGTATTTGTCACCATTATCAGTCGAGTTGGGTTACAGGAACTAATATGCTGGCCTCTGATTAGTCTTTTTGGCTTTTGCAGGTTGATTTACAGGTTAAGGTATGTGAAGCTGATTCTTATATTTCGGACTTAACCCGTCAAGTACGAGAGAACTATGCCACTATTTTGGTACTTGGATGTAGCCTTTCAAGGTATTGGATGAAATAAAAGATTCCTCCCTTTCACCCCTCTCTCCTTCTCCCTCTTAGTTTCTGATAAGGTGATTTGCTGCCTGCAGACCTGATTACTCAGTTTTCAATACCAGCCTGAAAGCCTTGCCTCCTACTTGCACTCTTATGGTGACGAACAATCTAGGCAGAATTCTACTTCAAAAGCAAGGAACTTCTCAACAAGGCTCCGAAAGAACGCTTTTACTATCTTCACACTCATCAATTTCCAAGTATGCTACAGGATATCAAGAAACTCGTATCTCTCACCTACATAAGTCTTTAGACCTACCGAGTTCTCTACCTTCATCATCATTACAAGCAAATGAAAACCAAGAAATCTCCCTAGCCAGCAAGAAAGTGCGGTCATTTCCTAAACTTGCAACTGGTGAATTGTTTCCTAAACTAGCGCTGCAGGATTCAAAAGGAATCAAAAGGCATTTTACATATGAACAGCTCAAATGTGCAACCAACAATTTTGATCCTGAAATGGTGATTGGTGAAGGTGGTCATAGCAAGGTGTACAAGGCCAACCTTGAAGATGGGCAAGCAGTAGCAGTGAAAGTTCTAAGCTCAGATTCGTCATCCGACGACCTTGTTCAAGAAGTCAAAATCTTGTCTGAGATAAAACATGAGAACATAGTTCACATTGTTGGGTTTTGTAATGACAAAAACATGCAGGCAATTGTGTACAATTTGCTTAAAGGAAGCCTAAAGCAGTACCTAAGAAAACTGGAGTGGAAGGAGAGAGTGGGGATTGCAATTGGGATAGCAAAAGCACTTAACTACCTTCATCATTCTTGCGACCCTCCAATCATCCACAGAGATGTAAAATCATCAAACATCCTCCTCTTTGACAACTTCCAACCACAAGTAAGTCAATCACATGGCAAACTATTAAAGGTCCAACAAACAAACAATCTACATTTACAATTCTTCTCCTCTAAACCACAACCTAAATTTGGTTTTCCCACTCCTAGTTAGCAGACTTTGGAGAAGCAACAGTGCTCAACAAACAGTCTGAAAATGCACAATCCAACACAAAGCCAAACAACATTGTTGGGACATTTGGATACTTAGCTCCAGAGTACATCATGTATGGAAAAATTGATGAGAAAATAGATGTCTACTCCTATGGGGTTGTACTTCTAGAACTCATCACAGGCAAAGAGGCAATTCATACAGATCAGAACAATCATTCAAGCTTGGTACTCTGGGTAATAACGAAAACAACAATTTCTATTGCACAATTAATCGATGCCCATAAGCAGAAACAACACAATTCAATCTTCATTGATCTTTTTTTTTTTTTCACTTACAGGCAAGGTCCCTACTCGGCCGCGGCCTGGGCGATCGACTAATTGACCCTGGCTTGAAGAACTACGAGAAGGAAGAAATGGCGGCAATGATGATGGCGGCACGCCTTTGCCTGTTGTTCACTTCTTCGAGGAGGCCAACAATGAAAACTGTAAATCTTTGGTTTTTCTCAGTATTTCCATCAATGGGTAACTGTGCAGATTGATTTTATTGGGCTTGTTGATTGTTTGTTCAACAGATATTGAGGCTGTTTGAAGAACCAAGGTACCTGCTTAAGATGCAGAAGGTAAGAGATGAGATCTTTAATGGCGGAAGTACAAAGGAAGTGGCCGATTTATGGAGAAGTGATGAACCAGACGACGAAGGAAATATTTTTAAAGTTGTTTCAGTATATTGCTTTAGGAAATCTGAAAATATAAATTTGAAAACAACGAATTAACGTATG

mRNA sequence

ATGTTGTCCTTCATCAGGCCCAAGACTCAGAAGAAGAGAACAATTATCTTTGGCCTAAAATCTGACAATGGAAGCAGAGATATTCTCCTTTGGTTGCTTACGACGATTGTCAATCCGGGAGATTATGTTCTGGCTATTCATGTTCAGGAGCCAAATGACAGTTTCAATCCAAATACTTTCCACATCCATGAAGATATTTGCAAATCCAAACAGGTTGATTTACAGGTTAAGGTATGTGAAGCTGATTCTTATATTTCGGACTTAACCCGTCAAGTACGAGAGAACTATGCCACTATTTTGGTACTTGGATGTAGCCTTTCAAGACCTGATTACTCAGTTTTCAATACCAGCCTGAAAGCCTTGCCTCCTACTTGCACTCTTATGGTGACGAACAATCTAGGCAGAATTCTACTTCAAAAGCAAGGAACTTCTCAACAAGGCTCCGAAAGAACGCTTTTACTATCTTCACACTCATCAATTTCCAAGTATGCTACAGGATATCAAGAAACTCGTATCTCTCACCTACATAAGTCTTTAGACCTACCGAGTTCTCTACCTTCATCATCATTACAAGCAAATGAAAACCAAGAAATCTCCCTAGCCAGCAAGAAAGTGCGGTCATTTCCTAAACTTGCAACTGGTGAATTGTTTCCTAAACTAGCGCTGCAGGATTCAAAAGGAATCAAAAGGCATTTTACATATGAACAGCTCAAATGTGCAACCAACAATTTTGATCCTGAAATGGTGATTGGTGAAGGTGGTCATAGCAAGGTGTACAAGGCCAACCTTGAAGATGGGCAAGCAGTAGCAGTGAAAGTTCTAAGCTCAGATTCGTCATCCGACGACCTTGTTCAAGAAGTCAAAATCTTGTCTGAGATAAAACATGAGAACATAGTTCACATTGTTGGGTTTTGTAATGACAAAAACATGCAGGCAATTGTGTACAATTTGCTTAAAGGAAGCCTAAAGCAGTACCTAAGAAAACTGGAGTGGAAGGAGAGAGTGGGGATTGCAATTGGGATAGCAAAAGCACTTAACTACCTTCATCATTCTTGCGACCCTCCAATCATCCACAGAGATGTAAAATCATCAAACATCCTCCTCTTTGACAACTTCCAACCACAATTAGCAGACTTTGGAGAAGCAACAGTGCTCAACAAACAGTCTGAAAATGCACAATCCAACACAAAGCCAAACAACATTGTTGGGACATTTGGATACTTAGCTCCAGAGTACATCATGTATGGAAAAATTGATGAGAAAATAGATGTCTACTCCTATGGGGTTGTACTTCTAGAACTCATCACAGGCAAAGAGGCAATTCATACAGATCAGAACAATCATTCAAGCTTGGTACTCTGGGCAAGGTCCCTACTCGGCCGCGGCCTGGGCGATCGACTAATTGACCCTGGCTTGAAGAACTACGAGAAGGAAGAAATGGCGGCAATGATGATGGCGGCACGCCTTTGCCTGTTGTTCACTTCTTCGAGGAGGCCAACAATGAAAACTATATTGAGGCTGTTTGAAGAACCAAGGTACCTGCTTAAGATGCAGAAGGTAAGAGATGAGATCTTTAATGGCGGAAGTACAAAGGAAGTGGCCGATTTATGGAGAAGTGATGAACCAGACGACGAAGGAAATATTTTTAAAGTTGTTTCAGTATATTGCTTTAGGAAATCTGAAAATATAAATTTGAAAACAACGAATTAACGTATG

Coding sequence (CDS)

ATGTTGTCCTTCATCAGGCCCAAGACTCAGAAGAAGAGAACAATTATCTTTGGCCTAAAATCTGACAATGGAAGCAGAGATATTCTCCTTTGGTTGCTTACGACGATTGTCAATCCGGGAGATTATGTTCTGGCTATTCATGTTCAGGAGCCAAATGACAGTTTCAATCCAAATACTTTCCACATCCATGAAGATATTTGCAAATCCAAACAGGTTGATTTACAGGTTAAGGTATGTGAAGCTGATTCTTATATTTCGGACTTAACCCGTCAAGTACGAGAGAACTATGCCACTATTTTGGTACTTGGATGTAGCCTTTCAAGACCTGATTACTCAGTTTTCAATACCAGCCTGAAAGCCTTGCCTCCTACTTGCACTCTTATGGTGACGAACAATCTAGGCAGAATTCTACTTCAAAAGCAAGGAACTTCTCAACAAGGCTCCGAAAGAACGCTTTTACTATCTTCACACTCATCAATTTCCAAGTATGCTACAGGATATCAAGAAACTCGTATCTCTCACCTACATAAGTCTTTAGACCTACCGAGTTCTCTACCTTCATCATCATTACAAGCAAATGAAAACCAAGAAATCTCCCTAGCCAGCAAGAAAGTGCGGTCATTTCCTAAACTTGCAACTGGTGAATTGTTTCCTAAACTAGCGCTGCAGGATTCAAAAGGAATCAAAAGGCATTTTACATATGAACAGCTCAAATGTGCAACCAACAATTTTGATCCTGAAATGGTGATTGGTGAAGGTGGTCATAGCAAGGTGTACAAGGCCAACCTTGAAGATGGGCAAGCAGTAGCAGTGAAAGTTCTAAGCTCAGATTCGTCATCCGACGACCTTGTTCAAGAAGTCAAAATCTTGTCTGAGATAAAACATGAGAACATAGTTCACATTGTTGGGTTTTGTAATGACAAAAACATGCAGGCAATTGTGTACAATTTGCTTAAAGGAAGCCTAAAGCAGTACCTAAGAAAACTGGAGTGGAAGGAGAGAGTGGGGATTGCAATTGGGATAGCAAAAGCACTTAACTACCTTCATCATTCTTGCGACCCTCCAATCATCCACAGAGATGTAAAATCATCAAACATCCTCCTCTTTGACAACTTCCAACCACAATTAGCAGACTTTGGAGAAGCAACAGTGCTCAACAAACAGTCTGAAAATGCACAATCCAACACAAAGCCAAACAACATTGTTGGGACATTTGGATACTTAGCTCCAGAGTACATCATGTATGGAAAAATTGATGAGAAAATAGATGTCTACTCCTATGGGGTTGTACTTCTAGAACTCATCACAGGCAAAGAGGCAATTCATACAGATCAGAACAATCATTCAAGCTTGGTACTCTGGGCAAGGTCCCTACTCGGCCGCGGCCTGGGCGATCGACTAATTGACCCTGGCTTGAAGAACTACGAGAAGGAAGAAATGGCGGCAATGATGATGGCGGCACGCCTTTGCCTGTTGTTCACTTCTTCGAGGAGGCCAACAATGAAAACTATATTGAGGCTGTTTGAAGAACCAAGGTACCTGCTTAAGATGCAGAAGGTAAGAGATGAGATCTTTAATGGCGGAAGTACAAAGGAAGTGGCCGATTTATGGAGAAGTGATGAACCAGACGACGAAGGAAATATTTTTAAAGTTGTTTCAGTATATTGCTTTAGGAAATCTGAAAATATAAATTTGAAAACAACGAATTAA

Protein sequence

MLSFIRPKTQKKRTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAIHVQEPNDSFNPNTFHIHEDICKSKQVDLQVKVCEADSYISDLTRQVRENYATILVLGCSLSRPDYSVFNTSLKALPPTCTLMVTNNLGRILLQKQGTSQQGSERTLLLSSHSSISKYATGYQETRISHLHKSLDLPSSLPSSSLQANENQEISLASKKVRSFPKLATGELFPKLALQDSKGIKRHFTYEQLKCATNNFDPEMVIGEGGHSKVYKANLEDGQAVAVKVLSSDSSSDDLVQEVKILSEIKHENIVHIVGFCNDKNMQAIVYNLLKGSLKQYLRKLEWKERVGIAIGIAKALNYLHHSCDPPIIHRDVKSSNILLFDNFQPQLADFGEATVLNKQSENAQSNTKPNNIVGTFGYLAPEYIMYGKIDEKIDVYSYGVVLLELITGKEAIHTDQNNHSSLVLWARSLLGRGLGDRLIDPGLKNYEKEEMAAMMMAARLCLLFTSSRRPTMKTILRLFEEPRYLLKMQKVRDEIFNGGSTKEVADLWRSDEPDDEGNIFKVVSVYCFRKSENINLKTTN
Homology
BLAST of Lsi10G008050 vs. ExPASy Swiss-Prot
Match: Q9FFW5 (Proline-rich receptor-like protein kinase PERK8 OS=Arabidopsis thaliana OX=3702 GN=PERK8 PE=1 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 6.1e-55
Identity = 125/344 (36.34%), Postives = 197/344 (57.27%), Query Frame = 0

Query: 184 SLPSSSLQANENQEISLASKKVRSFPKLATGELFPKLALQDSKGI----KRHFTYEQLKC 243
           ++P S+  + +  ++ L + +  + PK+ +      +      G+    +  F+Y++L  
Sbjct: 275 TMPPSAYSSPQGSDVVLFNSRSSAPPKMRSHSGSDYMYASSDSGMVSNQRSWFSYDELSQ 334

Query: 244 ATNNFDPEMVIGEGGHSKVYKANLEDGQAVAVKVLSSDSSSD--DLVQEVKILSEIKHEN 303
            T+ F  + ++GEGG   VYK  L DG+ VAVK L    S    +   EV+I+S + H +
Sbjct: 335 VTSGFSEKNLLGEGGFGCVYKGVLSDGREVAVKQLKIGGSQGEREFKAEVEIISRVHHRH 394

Query: 304 IVHIVGFCNDKNMQAIVYNLLKGSLKQY------LRKLEWKERVGIAIGIAKALNYLHHS 363
           +V +VG+C  +  + +VY+ +  +   Y         + W+ RV +A G A+ + YLH  
Sbjct: 395 LVTLVGYCISEQHRLLVYDYVPNNTLHYHLHAPGRPVMTWETRVRVAAGAARGIAYLHED 454

Query: 364 CDPPIIHRDVKSSNILLFDNFQPQLADFGEATVLNKQSENAQSNTKPNNIVGTFGYLAPE 423
           C P IIHRD+KSSNILL ++F+  +ADFG A +  +   N   +T+   ++GTFGY+APE
Sbjct: 455 CHPRIIHRDIKSSNILLDNSFEALVADFGLAKIAQELDLNTHVSTR---VMGTFGYMAPE 514

Query: 424 YIMYGKIDEKIDVYSYGVVLLELITGKEAIHTDQN-NHSSLVLWARSLLGRGLG----DR 483
           Y   GK+ EK DVYSYGV+LLELITG++ + T Q     SLV WAR LLG+ +     D 
Sbjct: 515 YATSGKLSEKADVYSYGVILLELITGRKPVDTSQPLGDESLVEWARPLLGQAIENEEFDE 574

Query: 484 LIDPGL-KNYEKEEMAAMMMAARLCLLFTSSRRPTMKTILRLFE 510
           L+DP L KN+   EM  M+ AA  C+  ++++RP M  ++R  +
Sbjct: 575 LVDPRLGKNFIPGEMFRMVEAAAACVRHSAAKRPKMSQVVRALD 615

BLAST of Lsi10G008050 vs. ExPASy Swiss-Prot
Match: Q9SGY7 (Putative proline-rich receptor-like protein kinase PERK11 OS=Arabidopsis thaliana OX=3702 GN=PERK11 PE=2 SV=2)

HSP 1 Score: 214.9 bits (546), Expect = 2.3e-54
Identity = 126/295 (42.71%), Postives = 177/295 (60.00%), Query Frame = 0

Query: 229 KRHFTYEQLKCATNNFDPEMVIGEGGHSKVYKANLEDGQAVAVKVLSSDSSSD--DLVQE 288
           K HFTYE+L   T  F    V+GEGG   VYK  L +G+ VA+K L S S+    +   E
Sbjct: 355 KIHFTYEELSQITEGFCKSFVVGEGGFGCVYKGILFEGKPVAIKQLKSVSAEGYREFKAE 414

Query: 289 VKILSEIKHENIVHIVGFCNDKNMQAIVYNLLKGSLKQY------LRKLEWKERVGIAIG 348
           V+I+S + H ++V +VG+C  +  + ++Y  +  +   Y      L  LEW  RV IAIG
Sbjct: 415 VEIISRVHHRHLVSLVGYCISEQHRFLIYEFVPNNTLDYHLHGKNLPVLEWSRRVRIAIG 474

Query: 349 IAKALNYLHHSCDPPIIHRDVKSSNILLFDNFQPQLADFGEATVLNKQSENAQSNTKPNN 408
            AK L YLH  C P IIHRD+KSSNILL D F+ Q+ADFG    L + ++ AQS+     
Sbjct: 475 AAKGLAYLHEDCHPKIIHRDIKSSNILLDDEFEAQVADFG----LARLNDTAQSHIS-TR 534

Query: 409 IVGTFGYLAPEYIMYGKIDEKIDVYSYGVVLLELITGKEAIHTDQN-NHSSLVLWARSLL 468
           ++GTFGYLAPEY   GK+ ++ DV+S+GVVLLELITG++ + T Q     SLV WAR  L
Sbjct: 535 VMGTFGYLAPEYASSGKLTDRSDVFSFGVVLLELITGRKPVDTSQPLGEESLVEWARPRL 594

Query: 469 GRGL--GD--RLIDPGLKN-YEKEEMAAMMMAARLCLLFTSSRRPTMKTILRLFE 510
              +  GD   ++DP L+N Y + E+  M+  A  C+  ++ +RP M  ++R  +
Sbjct: 595 IEAIEKGDISEVVDPRLENDYVESEVYKMIETAASCVRHSALKRPRMVQVVRALD 644

BLAST of Lsi10G008050 vs. ExPASy Swiss-Prot
Match: Q8H1D6 (Receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Arabidopsis thaliana OX=3702 GN=RBK1 PE=1 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 4.0e-54
Identity = 124/317 (39.12%), Postives = 190/317 (59.94%), Query Frame = 0

Query: 206 RSFPKLATGE-LFPKLALQDSKGIKRHFTYEQLKCATNNFDPEMVIGEGGHSKVYKANLE 265
           R  PKL   E  F   A   +K   R+FTYE+L  AT+ F+PE +IG+GGH++VYK  L 
Sbjct: 114 RKQPKLTPSESAFTCEAFFMAKPSWRNFTYEELAVATDYFNPENMIGKGGHAEVYKGVLI 173

Query: 266 DGQAVAVKVLSSDSSSD-----DLVQEVKILSEIKHENIVHIVGFCNDKNMQAIVYNLLK 325
           +G+ VA+K L S +  +     D + E+ I++ + H N   + GF +D+ +  ++     
Sbjct: 174 NGETVAIKKLMSHAKEEEERVSDFLSELGIIAHVNHPNAARLRGFSSDRGLHFVLEYAPY 233

Query: 326 GSLKQYL----RKLEWKERVGIAIGIAKALNYLHHSCDPPIIHRDVKSSNILLFDNFQPQ 385
           GSL   L      LEWK R  +A+GIA  L+YLH++C   IIHRD+K+SNILL  +++ Q
Sbjct: 234 GSLASMLFGSEECLEWKIRYKVALGIADGLSYLHNACPRRIIHRDIKASNILLNHDYEAQ 293

Query: 386 LADFGEATVLNKQSENAQSNTKPNNIV----GTFGYLAPEYIMYGKIDEKIDVYSYGVVL 445
           ++DFG A  L            P+++V    GTFGYLAPEY M+G +DEKIDV+++GV+L
Sbjct: 294 ISDFGLAKWL--------PENWPHHVVFPIEGTFGYLAPEYFMHGIVDEKIDVFAFGVLL 353

Query: 446 LELITGKEAIHTDQNNHSSLVLWARSLLGRGLGDRLIDPGLKN-YEKEEMAAMMMAARLC 505
           LE+IT + A+  D  +  S+V WA+  L +   + ++DP L N +   EM  +M+ A +C
Sbjct: 354 LEIITSRRAV--DTASRQSIVAWAKPFLEKNSMEDIVDPRLGNMFNPTEMQRVMLTASMC 413

Query: 506 LLFTSSRRPTMKTILRL 508
           +   ++ RP M  +++L
Sbjct: 414 VHHIAAMRPDMTRLVQL 420

BLAST of Lsi10G008050 vs. ExPASy Swiss-Prot
Match: Q9SX31 (Proline-rich receptor-like protein kinase PERK9 OS=Arabidopsis thaliana OX=3702 GN=PERK9 PE=1 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 2.6e-53
Identity = 136/323 (42.11%), Postives = 181/323 (56.04%), Query Frame = 0

Query: 201 ASKKVRSFPKLATGELFPKLALQDSKGIKRHFTYEQLKCATNNFDPEMVIGEGGHSKVYK 260
           ASK+  S+   + G       L +SK +   F+YE+L  ATN F  E ++GEGG   VYK
Sbjct: 344 ASKRSGSYQSQSGG-------LGNSKAL---FSYEELVKATNGFSQENLLGEGGFGCVYK 403

Query: 261 ANLEDGQAVAVKVLSSDSSSDD--LVQEVKILSEIKHENIVHIVGFCNDKNMQAIVYNLL 320
             L DG+ VAVK L       D     EV+ LS I H ++V IVG C   + + ++Y+ +
Sbjct: 404 GILPDGRVVAVKQLKIGGGQGDREFKAEVETLSRIHHRHLVSIVGHCISGDRRLLIYDYV 463

Query: 321 KGS-----LKQYLRKLEWKERVGIAIGIAKALNYLHHSCDPPIIHRDVKSSNILLFDNFQ 380
             +     L      L+W  RV IA G A+ L YLH  C P IIHRD+KSSNILL DNF 
Sbjct: 464 SNNDLYFHLHGEKSVLDWATRVKIAAGAARGLAYLHEDCHPRIIHRDIKSSNILLEDNFD 523

Query: 381 PQLADFGEATVLNKQSENAQSNTK-PNNIVGTFGYLAPEYIMYGKIDEKIDVYSYGVVLL 440
            +++DFG A +          NT     ++GTFGY+APEY   GK+ EK DV+S+GVVLL
Sbjct: 524 ARVSDFGLARLA------LDCNTHITTRVIGTFGYMAPEYASSGKLTEKSDVFSFGVVLL 583

Query: 441 ELITGKEAIHTDQN-NHSSLVLWARSLLGRGLG----DRLIDPGL-KNYEKEEMAAMMMA 500
           ELITG++ + T Q     SLV WAR L+   +     D L DP L  NY + EM  M+ A
Sbjct: 584 ELITGRKPVDTSQPLGDESLVEWARPLISHAIETEEFDSLADPKLGGNYVESEMFRMIEA 643

Query: 501 ARLCLLFTSSRRPTMKTILRLFE 510
           A  C+   +++RP M  I+R FE
Sbjct: 644 AGACVRHLATKRPRMGQIVRAFE 650

BLAST of Lsi10G008050 vs. ExPASy Swiss-Prot
Match: Q8RWW0 (Receptor-like serine/threonine-protein kinase ALE2 OS=Arabidopsis thaliana OX=3702 GN=ALE2 PE=1 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 3.3e-53
Identity = 114/283 (40.28%), Postives = 179/283 (63.25%), Query Frame = 0

Query: 232 FTYEQLKCATNNFDPEMVIGEGGHSKVYKANLEDGQAVAVKVLSSDSSSDD--LVQEVKI 291
           FT  +L+ AT+ F  + V+GEGG  +VY+ ++EDG  VAVK+L+ D+ + D   + EV++
Sbjct: 337 FTLSELEKATDRFSAKRVLGEGGFGRVYQGSMEDGTEVAVKLLTRDNQNRDREFIAEVEM 396

Query: 292 LSEIKHENIVHIVGFCNDKNMQAIVYNLL-KGSLKQYLRK--LEWKERVGIAIGIAKALN 351
           LS + H N+V ++G C +   + ++Y L+  GS++ +L +  L+W  R+ IA+G A+ L 
Sbjct: 397 LSRLHHRNLVKLIGICIEGRTRCLIYELVHNGSVESHLHEGTLDWDARLKIALGAARGLA 456

Query: 352 YLHHSCDPPIIHRDVKSSNILLFDNFQPQLADFGEATVLNKQSENAQSNTKPNNIVGTFG 411
           YLH   +P +IHRD K+SN+LL D+F P+++DFG A    + S++  +      ++GTFG
Sbjct: 457 YLHEDSNPRVIHRDFKASNVLLEDDFTPKVSDFGLAREATEGSQHIST-----RVMGTFG 516

Query: 412 YLAPEYIMYGKIDEKIDVYSYGVVLLELITGKEAIHTDQ-NNHSSLVLWARSLLGRGLG- 471
           Y+APEY M G +  K DVYSYGVVLLEL+TG+  +   Q +   +LV WAR LL    G 
Sbjct: 517 YVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRRPVDMSQPSGEENLVTWARPLLANREGL 576

Query: 472 DRLIDPGLK-NYEKEEMAAMMMAARLCLLFTSSRRPTMKTILR 507
           ++L+DP L   Y  ++MA +   A +C+    S RP M  +++
Sbjct: 577 EQLVDPALAGTYNFDDMAKVAAIASMCVHQEVSHRPFMGEVVQ 614

BLAST of Lsi10G008050 vs. ExPASy TrEMBL
Match: A0A6J1CFZ7 (receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Momordica charantia OX=3673 GN=LOC111011329 PE=4 SV=1)

HSP 1 Score: 870.2 bits (2247), Expect = 4.9e-249
Identity = 443/546 (81.14%), Postives = 484/546 (88.64%), Query Frame = 0

Query: 1   MLSFIRPKTQKKRTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAIHVQEPNDSFNPNTF 60
           MLS IR KT+KKRTIIFGLKSDNGSRDILLWLLTTI+NPGD VLA+HV+EPNDSFNPN+F
Sbjct: 1   MLSRIRSKTRKKRTIIFGLKSDNGSRDILLWLLTTIINPGDNVLAVHVREPNDSFNPNSF 60

Query: 61  HIHEDICKSKQVDLQVKVCEADSYISDLTRQVRENYATILVLGCSLSRPDYSVFNTSLKA 120
           HIHEDICKSKQVDLQVKV EADSYIS+LTRQVRENYATILVLGCSLSRPDYSVF++ LK 
Sbjct: 61  HIHEDICKSKQVDLQVKVSEADSYISNLTRQVRENYATILVLGCSLSRPDYSVFSSCLKG 120

Query: 121 LPPTCTLMVTNNLGRILLQKQGTSQQGSERTLLLSSHSSISKYATGYQETRISHLHKSLD 180
           LPPTCTLMV +NLGRILLQKQGTSQQGSE+ LL SS SS+SKYA GYQ+    HL KSL 
Sbjct: 121 LPPTCTLMVMDNLGRILLQKQGTSQQGSEKALLQSSQSSVSKYAAGYQQANSCHLQKSLT 180

Query: 181 LPSSLPSSSLQANENQEISLASKKVRSFPKLATGELFPKLALQDSKGIKRHFTYEQLKCA 240
           LPSSLPSSS QA+E QEISLA+KK R FP  A+ E FP+LALQDS+G  R FT EQL  A
Sbjct: 181 LPSSLPSSSRQASEKQEISLANKKARPFPGFASSEPFPRLALQDSRGTSREFTSEQLNRA 240

Query: 241 TNNFDPEMVIGEGGHSKVYKANLEDGQAVAVKVLSSDSSSDDLVQEVKILSEIKHENIVH 300
           TNNF+P MVIGEGGHSKVY+ANLEDGQA AVKVLSS SSS DLVQEV+I SEIKHENIV 
Sbjct: 241 TNNFNPVMVIGEGGHSKVYRANLEDGQAAAVKVLSSHSSSHDLVQEVEIFSEIKHENIVQ 300

Query: 301 IVGFCNDKNMQAIVYNLLKGSLKQYLRKLEWKERVGIAIGIAKALNYLHHSCDPPIIHRD 360
           I+GFCND+NM+AIVY+LLKGSLKQYLRK +W+ERVGIAIG+AKAL YLHHSC+PPIIHRD
Sbjct: 301 IIGFCNDRNMKAIVYSLLKGSLKQYLRKRKWRERVGIAIGVAKALTYLHHSCNPPIIHRD 360

Query: 361 VKSSNILLFDNFQPQLADFGEATVLNKQSENAQSNTKPNNIVGTFGYLAPEYIMYGKIDE 420
           VKSSNILLFDNFQPQLADFGEATVL +QS+NA+SN  P N+VGTFGYLAPEYIMYGK+DE
Sbjct: 361 VKSSNILLFDNFQPQLADFGEATVL-RQSQNAESNINPYNVVGTFGYLAPEYIMYGKVDE 420

Query: 421 KIDVYSYGVVLLELITGKEAIHTDQNNHSSLVLWARSLLGRGLGDRLIDPGLK-NYEKEE 480
           KIDVYSYGVVLLELITGKEAI TDQN+H SLVL ARSLLG GLGDRLIDP LK NY K E
Sbjct: 421 KIDVYSYGVVLLELITGKEAIQTDQNSHGSLVLRARSLLGFGLGDRLIDPNLKGNYNKVE 480

Query: 481 MAAMMMAARLCLLFTSSRRPTMKTILRLFEEPRYLLKMQKVRDEIFNGGSTKEVADLWRS 540
           M AMMMAARLCLL++SSRRP M+TIL+L EEP YL KMQ+ RDE+FNGGST+E   LWR 
Sbjct: 481 MRAMMMAARLCLLYSSSRRPIMETILKLLEEPEYLFKMQEARDELFNGGSTEEETYLWRY 540

Query: 541 DEPDDE 546
           DEPD E
Sbjct: 541 DEPDGE 545

BLAST of Lsi10G008050 vs. ExPASy TrEMBL
Match: A0A6J1I7A3 (PTI1-like tyrosine-protein kinase 1 OS=Cucurbita maxima OX=3661 GN=LOC111469794 PE=4 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 6.2e-220
Identity = 407/522 (77.97%), Postives = 440/522 (84.29%), Query Frame = 0

Query: 1   MLSFIRPKTQKKRTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAIHVQEPNDSFNPNTF 60
           MLS I+PKT+KKRTII GL SDNGSRDILLWLLTT+VNPGDYVLAIHVQEPNDSFNPNTF
Sbjct: 1   MLSLIKPKTRKKRTIIIGLNSDNGSRDILLWLLTTLVNPGDYVLAIHVQEPNDSFNPNTF 60

Query: 61  HIHEDICKSKQVDLQVKVCEADSYISDLTRQVRENYATILVLGCSLSRPDYSVFNTSLKA 120
           HIHEDICKSKQVDLQVKV +   YIS+LTRQVRE+YA+ILVLGCSLSRPDYSVF+T LK 
Sbjct: 61  HIHEDICKSKQVDLQVKVSKTAPYISNLTRQVRESYASILVLGCSLSRPDYSVFSTCLKG 120

Query: 121 LPPTCTLMVTNNLGRILLQKQGTSQQGSERTLLLSSHSSISKYATGYQETRISHLHKSLD 180
           LPPTCTLMV +NLG+IL Q QGTSQQGSER +L SSHS       GYQ+T I  L KS  
Sbjct: 121 LPPTCTLMVMDNLGKILFQNQGTSQQGSERAVLRSSHS-------GYQQTNIRDLQKSST 180

Query: 181 LPSSLPSSSLQANENQEISLASKKVRSFPKLATGELFP-KLALQDSKGIKRHFTYEQLKC 240
           L      SSLQANE Q+I   SKK + F   A  EL P   +LQ  K IKR FT EQL  
Sbjct: 181 L-----RSSLQANEKQKI---SKKEKPFLNFALHELLPSSRSLQYPKRIKRQFTSEQLNY 240

Query: 241 ATNNFDPEMVIGEGGHSKVYKANLEDGQAVAVKVLSSDSSSDDLVQEVKILSEIKHENIV 300
           ATNN DP MVIGEGGHSKVY+ANLEDGQA AVKVLSSDS SDD+VQEV+ILS+IKHENIV
Sbjct: 241 ATNNLDPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSDSQSDDVVQEVEILSKIKHENIV 300

Query: 301 HIVGFCNDKNMQAIVYNLLKGSLKQYLRKLEWKERVGIAIGIAKALNYLHHSCDPPIIHR 360
             +GFC+DKNMQAIVYNLLKGSL QYLRKLEWKERV IAIG+AKALN+LHHSC+PPIIHR
Sbjct: 301 QFIGFCSDKNMQAIVYNLLKGSLNQYLRKLEWKERVAIAIGVAKALNHLHHSCNPPIIHR 360

Query: 361 DVKSSNILLFDNFQPQLADFGEATVLNKQSENAQSNTKPNNIVGTFGYLAPEYIMYGKID 420
           DVKSSNILLFDNFQPQLADFGEAT L KQS+N QSNTKP  IVGTFGYLAPEYIMYGKID
Sbjct: 361 DVKSSNILLFDNFQPQLADFGEATEL-KQSQNEQSNTKPCKIVGTFGYLAPEYIMYGKID 420

Query: 421 EKIDVYSYGVVLLELITGKEAIHTDQNNHSSLVLWARSLLGRGLGDRLIDPGLKNYEKEE 480
           EKID+YSYGVVLLELITGKEAI T+QN+H SLVLWAR LL  G G++LIDP LKNY KEE
Sbjct: 421 EKIDIYSYGVVLLELITGKEAIQTNQNSHGSLVLWARPLLSCGQGNQLIDPNLKNYNKEE 480

Query: 481 MAAMMMAARLCLLFTSSRRPTMKTILRLFEEPRYLLKMQKVR 522
           M AM+MAAR CLL +SSRRPTMKTILRLFEE  YL KMQKV+
Sbjct: 481 MEAMLMAARFCLLDSSSRRPTMKTILRLFEEQGYLFKMQKVQ 506

BLAST of Lsi10G008050 vs. ExPASy TrEMBL
Match: A0A6J1G7M9 (PTI1-like tyrosine-protein kinase 1 OS=Cucurbita moschata OX=3662 GN=LOC111451486 PE=4 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 2.5e-216
Identity = 402/522 (77.01%), Postives = 435/522 (83.33%), Query Frame = 0

Query: 1   MLSFIRPKTQKKRTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAIHVQEPNDSFNPNTF 60
           MLS I+PKT+KKRTII GL SDNGSRDILLWLLT +VNPGDYVLAIHVQEPNDSFNPNTF
Sbjct: 1   MLSLIKPKTRKKRTIIVGLNSDNGSRDILLWLLTALVNPGDYVLAIHVQEPNDSFNPNTF 60

Query: 61  HIHEDICKSKQVDLQVKVCEADSYISDLTRQVRENYATILVLGCSLSRPDYSVFNTSLKA 120
           HIHEDICKSKQVDLQVKV E   YIS+LTRQVRE+YA+ILVLGCSLSRPDYSVF+T LK 
Sbjct: 61  HIHEDICKSKQVDLQVKVSETTPYISNLTRQVRESYASILVLGCSLSRPDYSVFSTCLKG 120

Query: 121 LPPTCTLMVTNNLGRILLQKQGTSQQGSERTLLLSSHSSISKYATGYQETRISHLHKSLD 180
           LPPTCTLMV +NLGRIL Q QGTSQQGSER    SSHS       GYQ+T I  L KS  
Sbjct: 121 LPPTCTLMVMDNLGRILFQNQGTSQQGSER----SSHS-------GYQQTNIRDLQKSST 180

Query: 181 LPSSLPSSSLQANENQEISLASKKVRSFPKLATGELFPKLALQD-SKGIKRHFTYEQLKC 240
           LP     SSLQANE Q+I   SKK + F   A  EL P    Q  S  IKR FT EQL  
Sbjct: 181 LP-----SSLQANEKQKI---SKKAQPFLNFAPHELLPSSRSQQYSTRIKRQFTSEQLNY 240

Query: 241 ATNNFDPEMVIGEGGHSKVYKANLEDGQAVAVKVLSSDSSSDDLVQEVKILSEIKHENIV 300
           ATNN DP M+IGEGGHSKVY+ANLEDGQA AVK+LSSDS SD+LVQEV+ILS+IKHENIV
Sbjct: 241 ATNNLDPAMLIGEGGHSKVYRANLEDGQAAAVKILSSDSPSDNLVQEVEILSKIKHENIV 300

Query: 301 HIVGFCNDKNMQAIVYNLLKGSLKQYLRKLEWKERVGIAIGIAKALNYLHHSCDPPIIHR 360
             +GFC+DKNMQA+VYNLLKGSL QYLRK+EWKERVGIAIG+AKALN+LHHSC+PPIIHR
Sbjct: 301 QFIGFCSDKNMQAVVYNLLKGSLNQYLRKMEWKERVGIAIGVAKALNHLHHSCNPPIIHR 360

Query: 361 DVKSSNILLFDNFQPQLADFGEATVLNKQSENAQSNTKPNNIVGTFGYLAPEYIMYGKID 420
           DVKSSNILLF NFQPQLADFGEAT L KQS+N QSNTKP  IVGTFGYLAPEYIMYGKID
Sbjct: 361 DVKSSNILLFGNFQPQLADFGEATEL-KQSQNGQSNTKPCKIVGTFGYLAPEYIMYGKID 420

Query: 421 EKIDVYSYGVVLLELITGKEAIHTDQNNHSSLVLWARSLLGRGLGDRLIDPGLKNYEKEE 480
           EKID+YSYGVVLLELITGKEAI T+QN+H SLVLWAR LL  G G++LIDP LKNY KEE
Sbjct: 421 EKIDIYSYGVVLLELITGKEAIQTNQNSHGSLVLWARPLLSCGQGNQLIDPNLKNYNKEE 480

Query: 481 MAAMMMAARLCLLFTSSRRPTMKTILRLFEEPRYLLKMQKVR 522
           M AM+MAARLCLL +SSRRPTMKTILRL EE  YL KM KV+
Sbjct: 481 MEAMLMAARLCLLDSSSRRPTMKTILRLLEEQGYLFKMLKVQ 502

BLAST of Lsi10G008050 vs. ExPASy TrEMBL
Match: A0A2P5CKG1 (Serine/threonine protein kinase OS=Trema orientale OX=63057 GN=TorRG33x02_281770 PE=4 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 2.0e-178
Identity = 336/547 (61.43%), Postives = 412/547 (75.32%), Query Frame = 0

Query: 2   LSFIRPKTQKKRTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAIHVQEPNDSFNPNTFH 61
           L+F     ++KRTII GLKSDN SR+ILL LLT +V P D VLAIHVQ+ +D+F+PNTFH
Sbjct: 12  LTFRSKADKEKRTIIIGLKSDNYSREILLRLLTLVVKPEDSVLAIHVQDSDDTFDPNTFH 71

Query: 62  IHEDICKSKQVDLQVKVCEADSYISDLTRQVRENYATILVLGCSLSRPDYSVFNTSLKAL 121
           IHED+CKSKQVD QVKVC  DSYIS LT QVR +YATIL LG SLS P+ S  N  LKAL
Sbjct: 72  IHEDLCKSKQVDFQVKVCIGDSYISVLTHQVRLSYATILALGSSLSGPNVSALNYCLKAL 131

Query: 122 PPTCTLMVTNNLGRILLQKQGTSQQGSERTLLLSSHSSISKYATGYQETRISHLHKSLDL 181
           PPTCTL+V NN+GRILLQ+ GTSQQGS R  L  S SS S   T     R   L KSL  
Sbjct: 132 PPTCTLLVLNNVGRILLQRPGTSQQGSRRVHLRQSLSSPSYTRTSQSSVRDRRLRKSLTA 191

Query: 182 PSSLPSSSLQANENQEI--SLAS---KKVRSFPKLATGELFPKLALQDSKGIKRHFTYEQ 241
           PS   ++     +++ I  SL S   KK    P L   +LF +L++ + +G  +HF+  +
Sbjct: 192 PSPSTTAPFLLQQSKRIAGSLCSSDLKKTTPVPNLMAQKLFQRLSILEGEGCSKHFSLTE 251

Query: 242 LKCATNNFDPEMVIGEGGHSKVYKANLEDGQAVAVKVLSSDS-SSDDLVQEVKILSEIKH 301
           L+ ATNNF P M+IGEGG S+VY+A LEDGQ  AVKVL + + +++DL++E+ +LS +KH
Sbjct: 252 LRWATNNFSPIMLIGEGGQSRVYRAILEDGQTAAVKVLKTTTWAAEDLLREIDLLSRLKH 311

Query: 302 ENIVHIVGFCNDKNMQAIVYNLLKGSLKQYLRKLEWKERVGIAIGIAKALNYLHHSCDPP 361
           ENIV I+G+C+   +QAIVY LL+GSLK+ LR L+W +R+ +AIG+AKALNYLHHSC+PP
Sbjct: 312 ENIVEIIGYCDCGELQAIVYELLRGSLKENLRMLDWSQRMSVAIGVAKALNYLHHSCNPP 371

Query: 362 IIHRDVKSSNILLFDNFQPQLADFGEATVLNKQSENAQSNTKPNNIVGTFGYLAPEYIMY 421
           IIHRDVKSSNILL D+FQPQL+DFG A  L +QS  + +N+KP ++VGTFGYLAPEY+MY
Sbjct: 372 IIHRDVKSSNILLSDDFQPQLSDFGSAIAL-RQSHQSSANSKPLSVVGTFGYLAPEYMMY 431

Query: 422 GKIDEKIDVYSYGVVLLELITGKEAIHTDQNNHSSLVLWARSLLGRGLGDRLIDPGL-KN 481
           GKIDEKIDVYSYGVVLLELITGKEAI TD+  H SLVLWARS+L  GL +RLIDP L  +
Sbjct: 432 GKIDEKIDVYSYGVVLLELITGKEAIQTDRAVHESLVLWARSMLSYGLCERLIDPYLYDD 491

Query: 482 YEKEEMAAMMMAARLCLLFTSSRRPTMKTILRLFEEPRYLLKMQKVRDEIFNGGSTKEVA 541
           Y KEEM  MM+AARLCL+ +SSRRPTMKTILRLFEEP Y + MQ+ RDE  NG S+K   
Sbjct: 492 YNKEEMKLMMVAARLCLMHSSSRRPTMKTILRLFEEPEYWISMQRERDEFLNGISSKCEI 551

BLAST of Lsi10G008050 vs. ExPASy TrEMBL
Match: A0A5J5BWU5 (Protein kinase domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_020959 PE=4 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 3.5e-178
Identity = 332/549 (60.47%), Postives = 421/549 (76.68%), Query Frame = 0

Query: 1   MLSFIRPKTQKKRTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAIHVQEPNDSFNPNTF 60
           MLSF+R +T KK+T + GLKSDN SR++LL LL  +V  GDYVLAIHVQEPND F+PNTF
Sbjct: 12  MLSFLRTRTTKKKTFVVGLKSDNCSREMLLRLLNLVVMSGDYVLAIHVQEPNDIFDPNTF 71

Query: 61  HIHEDICKSKQVDLQVKVCEADSYISDLTRQVRENYATILVLGCSLSRPDYSVFNTSLKA 120
           HIHED+CKSKQVD QVKVC  +SYI++LT QVR N+ATILVLGCS   P  SV    LKA
Sbjct: 72  HIHEDLCKSKQVDFQVKVCIGNSYIAELTHQVRINFATILVLGCSTPWPKDSVVANCLKA 131

Query: 121 LPPTCTLMVTNNLGRILLQKQGTSQQGSERTLLLSSHSSISKYATGYQETRISHLHKSLD 180
           LPPTC+L+V +N GR+L+Q+ GTSQQGS   +L SS S++S++    Q      + KSL 
Sbjct: 132 LPPTCSLLVMDNGGRVLVQRPGTSQQGSTNKVLQSSLSAVSRFPNCEQSGTSRQIKKSLT 191

Query: 181 LPSSLPSSSLQANENQEISLASKKVRSFPKLATGELFPKLALQDSKGIKRHFTYEQLKCA 240
           +PSS  SS L+  EN       K ++  P   T ++  KLA  ++KG    FT  +L CA
Sbjct: 192 MPSSSTSSLLRQTENMRQQRVRKSLQ-LPDFMTQKMLQKLATLETKGSGSRFTSRELNCA 251

Query: 241 TNNFDPEMVIGEGGHSKVYKANLEDGQAVAVKVL-SSDSSSDDLVQEVKILSEIKHENIV 300
           T NF  EMVIGEGG+SKVY+AN+EDGQA AVKVL ++ SS++DL +EV++LS +KHENI+
Sbjct: 252 TYNFSTEMVIGEGGNSKVYRANIEDGQAAAVKVLKTTPSSAEDLFREVEMLSGLKHENII 311

Query: 301 HIVGFCNDKNMQAIVYNLLKGSLKQYLRKLEWKERVGIAIGIAKALNYLHHSCDPPIIHR 360
            ++G+C  K + A+VYNLLKGSL+Q L +L+W ER+ IAIG+AKAL+YL HSC PPIIHR
Sbjct: 312 QLIGYCYSKELHAVVYNLLKGSLRQKLNQLKWSERMMIAIGVAKALDYL-HSCSPPIIHR 371

Query: 361 DVKSSNILLFDNFQPQLADFGEATVLNKQSENAQSNTKPNNIVGTFGYLAPEYIMYGKID 420
           D+KSSNILL DN QP L+DFG A ++++Q++ + +  KP ++VGTFGYLAPEY+MYGK+D
Sbjct: 372 DIKSSNILLSDNCQPVLSDFG-AAMVHQQTQQSSAYAKPFHVVGTFGYLAPEYMMYGKVD 431

Query: 421 EKIDVYSYGVVLLELITGKEAIHTDQ-NNHSSLVLWARSLLGRGLGDRLIDPGLK-NYEK 480
           EK+DVYSYGVVLLELITGKEAI T+Q +NH SLVLWARSLLG GL +RLIDP LK +Y K
Sbjct: 432 EKVDVYSYGVVLLELITGKEAIQTNQTSNHESLVLWARSLLGCGLCERLIDPYLKEDYNK 491

Query: 481 EEMAAMMMAARLCLLFTSSRRPTMKTILRLFEEPRYLLKMQKVRDEIFNGGSTKEVADLW 540
           +EM  MM+AARLCLL +SSRRP MKTILRLFEEP + LKMQ+ ++E+ N  S++  + LW
Sbjct: 492 DEMKTMMIAARLCLLHSSSRRPPMKTILRLFEEPEHWLKMQRKKEELLNRISSRGESGLW 551

Query: 541 RSDEPDDEG 547
           R+D+ D  G
Sbjct: 552 RNDDSDASG 557

BLAST of Lsi10G008050 vs. NCBI nr
Match: XP_022140740.1 (receptor-like cytosolic serine/threonine-protein kinase RBK1 [Momordica charantia])

HSP 1 Score: 870.2 bits (2247), Expect = 1.0e-248
Identity = 443/546 (81.14%), Postives = 484/546 (88.64%), Query Frame = 0

Query: 1   MLSFIRPKTQKKRTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAIHVQEPNDSFNPNTF 60
           MLS IR KT+KKRTIIFGLKSDNGSRDILLWLLTTI+NPGD VLA+HV+EPNDSFNPN+F
Sbjct: 1   MLSRIRSKTRKKRTIIFGLKSDNGSRDILLWLLTTIINPGDNVLAVHVREPNDSFNPNSF 60

Query: 61  HIHEDICKSKQVDLQVKVCEADSYISDLTRQVRENYATILVLGCSLSRPDYSVFNTSLKA 120
           HIHEDICKSKQVDLQVKV EADSYIS+LTRQVRENYATILVLGCSLSRPDYSVF++ LK 
Sbjct: 61  HIHEDICKSKQVDLQVKVSEADSYISNLTRQVRENYATILVLGCSLSRPDYSVFSSCLKG 120

Query: 121 LPPTCTLMVTNNLGRILLQKQGTSQQGSERTLLLSSHSSISKYATGYQETRISHLHKSLD 180
           LPPTCTLMV +NLGRILLQKQGTSQQGSE+ LL SS SS+SKYA GYQ+    HL KSL 
Sbjct: 121 LPPTCTLMVMDNLGRILLQKQGTSQQGSEKALLQSSQSSVSKYAAGYQQANSCHLQKSLT 180

Query: 181 LPSSLPSSSLQANENQEISLASKKVRSFPKLATGELFPKLALQDSKGIKRHFTYEQLKCA 240
           LPSSLPSSS QA+E QEISLA+KK R FP  A+ E FP+LALQDS+G  R FT EQL  A
Sbjct: 181 LPSSLPSSSRQASEKQEISLANKKARPFPGFASSEPFPRLALQDSRGTSREFTSEQLNRA 240

Query: 241 TNNFDPEMVIGEGGHSKVYKANLEDGQAVAVKVLSSDSSSDDLVQEVKILSEIKHENIVH 300
           TNNF+P MVIGEGGHSKVY+ANLEDGQA AVKVLSS SSS DLVQEV+I SEIKHENIV 
Sbjct: 241 TNNFNPVMVIGEGGHSKVYRANLEDGQAAAVKVLSSHSSSHDLVQEVEIFSEIKHENIVQ 300

Query: 301 IVGFCNDKNMQAIVYNLLKGSLKQYLRKLEWKERVGIAIGIAKALNYLHHSCDPPIIHRD 360
           I+GFCND+NM+AIVY+LLKGSLKQYLRK +W+ERVGIAIG+AKAL YLHHSC+PPIIHRD
Sbjct: 301 IIGFCNDRNMKAIVYSLLKGSLKQYLRKRKWRERVGIAIGVAKALTYLHHSCNPPIIHRD 360

Query: 361 VKSSNILLFDNFQPQLADFGEATVLNKQSENAQSNTKPNNIVGTFGYLAPEYIMYGKIDE 420
           VKSSNILLFDNFQPQLADFGEATVL +QS+NA+SN  P N+VGTFGYLAPEYIMYGK+DE
Sbjct: 361 VKSSNILLFDNFQPQLADFGEATVL-RQSQNAESNINPYNVVGTFGYLAPEYIMYGKVDE 420

Query: 421 KIDVYSYGVVLLELITGKEAIHTDQNNHSSLVLWARSLLGRGLGDRLIDPGLK-NYEKEE 480
           KIDVYSYGVVLLELITGKEAI TDQN+H SLVL ARSLLG GLGDRLIDP LK NY K E
Sbjct: 421 KIDVYSYGVVLLELITGKEAIQTDQNSHGSLVLRARSLLGFGLGDRLIDPNLKGNYNKVE 480

Query: 481 MAAMMMAARLCLLFTSSRRPTMKTILRLFEEPRYLLKMQKVRDEIFNGGSTKEVADLWRS 540
           M AMMMAARLCLL++SSRRP M+TIL+L EEP YL KMQ+ RDE+FNGGST+E   LWR 
Sbjct: 481 MRAMMMAARLCLLYSSSRRPIMETILKLLEEPEYLFKMQEARDELFNGGSTEEETYLWRY 540

Query: 541 DEPDDE 546
           DEPD E
Sbjct: 541 DEPDGE 545

BLAST of Lsi10G008050 vs. NCBI nr
Match: XP_022970989.1 (PTI1-like tyrosine-protein kinase 1 [Cucurbita maxima])

HSP 1 Score: 773.5 bits (1996), Expect = 1.3e-219
Identity = 407/522 (77.97%), Postives = 440/522 (84.29%), Query Frame = 0

Query: 1   MLSFIRPKTQKKRTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAIHVQEPNDSFNPNTF 60
           MLS I+PKT+KKRTII GL SDNGSRDILLWLLTT+VNPGDYVLAIHVQEPNDSFNPNTF
Sbjct: 1   MLSLIKPKTRKKRTIIIGLNSDNGSRDILLWLLTTLVNPGDYVLAIHVQEPNDSFNPNTF 60

Query: 61  HIHEDICKSKQVDLQVKVCEADSYISDLTRQVRENYATILVLGCSLSRPDYSVFNTSLKA 120
           HIHEDICKSKQVDLQVKV +   YIS+LTRQVRE+YA+ILVLGCSLSRPDYSVF+T LK 
Sbjct: 61  HIHEDICKSKQVDLQVKVSKTAPYISNLTRQVRESYASILVLGCSLSRPDYSVFSTCLKG 120

Query: 121 LPPTCTLMVTNNLGRILLQKQGTSQQGSERTLLLSSHSSISKYATGYQETRISHLHKSLD 180
           LPPTCTLMV +NLG+IL Q QGTSQQGSER +L SSHS       GYQ+T I  L KS  
Sbjct: 121 LPPTCTLMVMDNLGKILFQNQGTSQQGSERAVLRSSHS-------GYQQTNIRDLQKSST 180

Query: 181 LPSSLPSSSLQANENQEISLASKKVRSFPKLATGELFP-KLALQDSKGIKRHFTYEQLKC 240
           L      SSLQANE Q+I   SKK + F   A  EL P   +LQ  K IKR FT EQL  
Sbjct: 181 L-----RSSLQANEKQKI---SKKEKPFLNFALHELLPSSRSLQYPKRIKRQFTSEQLNY 240

Query: 241 ATNNFDPEMVIGEGGHSKVYKANLEDGQAVAVKVLSSDSSSDDLVQEVKILSEIKHENIV 300
           ATNN DP MVIGEGGHSKVY+ANLEDGQA AVKVLSSDS SDD+VQEV+ILS+IKHENIV
Sbjct: 241 ATNNLDPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSDSQSDDVVQEVEILSKIKHENIV 300

Query: 301 HIVGFCNDKNMQAIVYNLLKGSLKQYLRKLEWKERVGIAIGIAKALNYLHHSCDPPIIHR 360
             +GFC+DKNMQAIVYNLLKGSL QYLRKLEWKERV IAIG+AKALN+LHHSC+PPIIHR
Sbjct: 301 QFIGFCSDKNMQAIVYNLLKGSLNQYLRKLEWKERVAIAIGVAKALNHLHHSCNPPIIHR 360

Query: 361 DVKSSNILLFDNFQPQLADFGEATVLNKQSENAQSNTKPNNIVGTFGYLAPEYIMYGKID 420
           DVKSSNILLFDNFQPQLADFGEAT L KQS+N QSNTKP  IVGTFGYLAPEYIMYGKID
Sbjct: 361 DVKSSNILLFDNFQPQLADFGEATEL-KQSQNEQSNTKPCKIVGTFGYLAPEYIMYGKID 420

Query: 421 EKIDVYSYGVVLLELITGKEAIHTDQNNHSSLVLWARSLLGRGLGDRLIDPGLKNYEKEE 480
           EKID+YSYGVVLLELITGKEAI T+QN+H SLVLWAR LL  G G++LIDP LKNY KEE
Sbjct: 421 EKIDIYSYGVVLLELITGKEAIQTNQNSHGSLVLWARPLLSCGQGNQLIDPNLKNYNKEE 480

Query: 481 MAAMMMAARLCLLFTSSRRPTMKTILRLFEEPRYLLKMQKVR 522
           M AM+MAAR CLL +SSRRPTMKTILRLFEE  YL KMQKV+
Sbjct: 481 MEAMLMAARFCLLDSSSRRPTMKTILRLFEEQGYLFKMQKVQ 506

BLAST of Lsi10G008050 vs. NCBI nr
Match: XP_023532060.1 (PTI1-like tyrosine-protein kinase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 771.9 bits (1992), Expect = 3.7e-219
Identity = 406/522 (77.78%), Postives = 438/522 (83.91%), Query Frame = 0

Query: 1   MLSFIRPKTQKKRTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAIHVQEPNDSFNPNTF 60
           MLS I+PKT+K+RTII GL SDNGSRDILLWLLTT+VNPGDYVLAIHVQEPNDSFNPNTF
Sbjct: 1   MLSLIKPKTRKRRTIIVGLNSDNGSRDILLWLLTTLVNPGDYVLAIHVQEPNDSFNPNTF 60

Query: 61  HIHEDICKSKQVDLQVKVCEADSYISDLTRQVRENYATILVLGCSLSRPDYSVFNTSLKA 120
           HIHEDICKSKQVDLQVKV E   YIS+LTRQVRE+YA+ILVLGCSLSRPDYSVF+T LK 
Sbjct: 61  HIHEDICKSKQVDLQVKVSETAPYISNLTRQVRESYASILVLGCSLSRPDYSVFSTCLKG 120

Query: 121 LPPTCTLMVTNNLGRILLQKQGTSQQGSERTLLLSSHSSISKYATGYQETRISHLHKSLD 180
           LPPTCTLMV +NLG+IL Q QGTSQQGSER +L SSHS       GYQ+T I  L KS  
Sbjct: 121 LPPTCTLMVMDNLGKILFQNQGTSQQGSERAVLRSSHS-------GYQQTNIRDLQKSST 180

Query: 181 LPSSLPSSSLQANENQEISLASKKVRSFPKLATGELFPKLALQD-SKGIKRHFTYEQLKC 240
           LP     SSLQ NE Q+I   SKK + F   A  EL P    Q  S  IKR FT EQL  
Sbjct: 181 LP-----SSLQENERQKI---SKKAQPFLNFAPHELLPSSKSQQYSTRIKRQFTSEQLNY 240

Query: 241 ATNNFDPEMVIGEGGHSKVYKANLEDGQAVAVKVLSSDSSSDDLVQEVKILSEIKHENIV 300
           ATNN DP MVIGEGGHSKVY+ANLEDGQA AVKVLSSDS SDD+VQEV+ILS+IKHENIV
Sbjct: 241 ATNNLDPAMVIGEGGHSKVYRANLEDGQAAAVKVLSSDSQSDDVVQEVEILSKIKHENIV 300

Query: 301 HIVGFCNDKNMQAIVYNLLKGSLKQYLRKLEWKERVGIAIGIAKALNYLHHSCDPPIIHR 360
             +GFC+DKNMQAIVYNLLKGSL QYLRKLEWKERVGIAIG+AKALN+LHHSC+PPIIHR
Sbjct: 301 QFIGFCSDKNMQAIVYNLLKGSLNQYLRKLEWKERVGIAIGVAKALNHLHHSCNPPIIHR 360

Query: 361 DVKSSNILLFDNFQPQLADFGEATVLNKQSENAQSNTKPNNIVGTFGYLAPEYIMYGKID 420
           DVKSSNILLF NFQPQLADFGEAT L KQS+N QSNTKP  IVGTFGYLAPEYIMYGKID
Sbjct: 361 DVKSSNILLFGNFQPQLADFGEATQL-KQSQNEQSNTKPCKIVGTFGYLAPEYIMYGKID 420

Query: 421 EKIDVYSYGVVLLELITGKEAIHTDQNNHSSLVLWARSLLGRGLGDRLIDPGLKNYEKEE 480
           EKID+YSYGVVLLELITGKEAI T+QN+H SLVLWAR LL  G G++LIDP LKNY KEE
Sbjct: 421 EKIDIYSYGVVLLELITGKEAIQTNQNSHGSLVLWARPLLSCGQGNQLIDPNLKNYNKEE 480

Query: 481 MAAMMMAARLCLLFTSSRRPTMKTILRLFEEPRYLLKMQKVR 522
           M AM+MAARLCLL +SSRRPTMK ILRLFEE  YL KMQKV+
Sbjct: 481 MEAMLMAARLCLLDSSSRRPTMKKILRLFEEQGYLFKMQKVQ 506

BLAST of Lsi10G008050 vs. NCBI nr
Match: KAG6604957.1 (Alanine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 768.1 bits (1982), Expect = 5.4e-218
Identity = 405/520 (77.88%), Postives = 438/520 (84.23%), Query Frame = 0

Query: 1   MLSFIRPKTQKKRTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAIHVQEPNDSFNPNTF 60
           MLS I+PKT+KKRTII GL SDNGSRDILLWLLTT+VNPGDYVLAIHVQEPNDSF+PNTF
Sbjct: 1   MLSLIKPKTRKKRTIIVGLNSDNGSRDILLWLLTTLVNPGDYVLAIHVQEPNDSFSPNTF 60

Query: 61  HIHEDICKSKQVDLQVKVCEADSYISDLTRQVRENYATILVLGCSLSRPDYSVFNTSLKA 120
           HIHEDICKSKQVDLQVKV E   YIS+LTRQVRE+YA+ILVLGCSLSRPDYSVF+T LK 
Sbjct: 61  HIHEDICKSKQVDLQVKVSETTPYISNLTRQVRESYASILVLGCSLSRPDYSVFSTCLKG 120

Query: 121 LPPTCTLMVTNNLGRILLQKQGTSQQGSERTLLLSSHSSISKYATGYQETRISHLHKSLD 180
           LPPTCTLMV +NLGRIL Q QGTSQQGSER    SSHS       GYQ+T I  L KS  
Sbjct: 121 LPPTCTLMVMDNLGRILFQNQGTSQQGSER----SSHS-------GYQQTNIRDLQKSST 180

Query: 181 LPSSLPSSSLQANENQEISLASKKVRSFPKLATGELFPKLALQD-SKGIKRHFTYEQLKC 240
           LP     SSLQANE Q+I   SKK ++F   A  EL P    Q  S  IKR FT EQL  
Sbjct: 181 LP-----SSLQANEKQKI---SKKAQAFLNFAPHELLPSSRSQQYSTRIKRQFTSEQLNY 240

Query: 241 ATNNFDPEMVIGEGGHSKVYKANLEDGQAVAVKVLSSDSSSDDLVQEVKILSEIKHENIV 300
           ATNN DP M+IGEGGHSKVY+ANLEDGQA AVK+LSSDS SD+LVQEV+ILS+IKHENIV
Sbjct: 241 ATNNLDPAMLIGEGGHSKVYRANLEDGQAAAVKILSSDSPSDNLVQEVEILSKIKHENIV 300

Query: 301 HIVGFCNDKNMQAIVYNLLKGSLKQYLRKLEWKERVGIAIGIAKALNYLHHSCDPPIIHR 360
             +GFC+DKNMQAIVYNLLKGSL QYLRK+EWKERVGIAIG+AKALN+LHHSC+PPIIHR
Sbjct: 301 QFIGFCSDKNMQAIVYNLLKGSLNQYLRKMEWKERVGIAIGVAKALNHLHHSCNPPIIHR 360

Query: 361 DVKSSNILLFDNFQPQLADFGEATVLNKQSENAQSNTKPNNIVGTFGYLAPEYIMYGKID 420
           DVKSSNILLFDNFQPQLADFGEAT L KQS+N QSNTKP  IVGTFGYLAPEYIMYGKID
Sbjct: 361 DVKSSNILLFDNFQPQLADFGEATEL-KQSQNEQSNTKPCKIVGTFGYLAPEYIMYGKID 420

Query: 421 EKIDVYSYGVVLLELITGKEAIHTDQNNHSSLVLWARSLLGRGLGDRLIDPGLKNYEKEE 480
           EKID+YSYGVVLLELITGKEAI T+QN+H SLVLWAR LL  G G++LIDP LKNY KEE
Sbjct: 421 EKIDIYSYGVVLLELITGKEAIQTNQNSHGSLVLWARPLLSCGQGNQLIDPNLKNYNKEE 480

Query: 481 MAAMMMAARLCLLFTSSRRPTMKTILRLFEEPRYLLKMQK 520
           M AM+MAARLCLL +SSRRPTMKTILRLFEE  YL KMQK
Sbjct: 481 MEAMLMAARLCLLDSSSRRPTMKTILRLFEEQGYLFKMQK 500

BLAST of Lsi10G008050 vs. NCBI nr
Match: XP_022947700.1 (PTI1-like tyrosine-protein kinase 1 [Cucurbita moschata])

HSP 1 Score: 761.5 bits (1965), Expect = 5.1e-216
Identity = 402/522 (77.01%), Postives = 435/522 (83.33%), Query Frame = 0

Query: 1   MLSFIRPKTQKKRTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAIHVQEPNDSFNPNTF 60
           MLS I+PKT+KKRTII GL SDNGSRDILLWLLT +VNPGDYVLAIHVQEPNDSFNPNTF
Sbjct: 1   MLSLIKPKTRKKRTIIVGLNSDNGSRDILLWLLTALVNPGDYVLAIHVQEPNDSFNPNTF 60

Query: 61  HIHEDICKSKQVDLQVKVCEADSYISDLTRQVRENYATILVLGCSLSRPDYSVFNTSLKA 120
           HIHEDICKSKQVDLQVKV E   YIS+LTRQVRE+YA+ILVLGCSLSRPDYSVF+T LK 
Sbjct: 61  HIHEDICKSKQVDLQVKVSETTPYISNLTRQVRESYASILVLGCSLSRPDYSVFSTCLKG 120

Query: 121 LPPTCTLMVTNNLGRILLQKQGTSQQGSERTLLLSSHSSISKYATGYQETRISHLHKSLD 180
           LPPTCTLMV +NLGRIL Q QGTSQQGSER    SSHS       GYQ+T I  L KS  
Sbjct: 121 LPPTCTLMVMDNLGRILFQNQGTSQQGSER----SSHS-------GYQQTNIRDLQKSST 180

Query: 181 LPSSLPSSSLQANENQEISLASKKVRSFPKLATGELFPKLALQD-SKGIKRHFTYEQLKC 240
           LP     SSLQANE Q+I   SKK + F   A  EL P    Q  S  IKR FT EQL  
Sbjct: 181 LP-----SSLQANEKQKI---SKKAQPFLNFAPHELLPSSRSQQYSTRIKRQFTSEQLNY 240

Query: 241 ATNNFDPEMVIGEGGHSKVYKANLEDGQAVAVKVLSSDSSSDDLVQEVKILSEIKHENIV 300
           ATNN DP M+IGEGGHSKVY+ANLEDGQA AVK+LSSDS SD+LVQEV+ILS+IKHENIV
Sbjct: 241 ATNNLDPAMLIGEGGHSKVYRANLEDGQAAAVKILSSDSPSDNLVQEVEILSKIKHENIV 300

Query: 301 HIVGFCNDKNMQAIVYNLLKGSLKQYLRKLEWKERVGIAIGIAKALNYLHHSCDPPIIHR 360
             +GFC+DKNMQA+VYNLLKGSL QYLRK+EWKERVGIAIG+AKALN+LHHSC+PPIIHR
Sbjct: 301 QFIGFCSDKNMQAVVYNLLKGSLNQYLRKMEWKERVGIAIGVAKALNHLHHSCNPPIIHR 360

Query: 361 DVKSSNILLFDNFQPQLADFGEATVLNKQSENAQSNTKPNNIVGTFGYLAPEYIMYGKID 420
           DVKSSNILLF NFQPQLADFGEAT L KQS+N QSNTKP  IVGTFGYLAPEYIMYGKID
Sbjct: 361 DVKSSNILLFGNFQPQLADFGEATEL-KQSQNGQSNTKPCKIVGTFGYLAPEYIMYGKID 420

Query: 421 EKIDVYSYGVVLLELITGKEAIHTDQNNHSSLVLWARSLLGRGLGDRLIDPGLKNYEKEE 480
           EKID+YSYGVVLLELITGKEAI T+QN+H SLVLWAR LL  G G++LIDP LKNY KEE
Sbjct: 421 EKIDIYSYGVVLLELITGKEAIQTNQNSHGSLVLWARPLLSCGQGNQLIDPNLKNYNKEE 480

Query: 481 MAAMMMAARLCLLFTSSRRPTMKTILRLFEEPRYLLKMQKVR 522
           M AM+MAARLCLL +SSRRPTMKTILRL EE  YL KM KV+
Sbjct: 481 MEAMLMAARLCLLDSSSRRPTMKTILRLLEEQGYLFKMLKVQ 502

BLAST of Lsi10G008050 vs. TAIR 10
Match: AT2G16750.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 276.2 bits (705), Expect = 6.0e-74
Identity = 189/589 (32.09%), Postives = 313/589 (53.14%), Query Frame = 0

Query: 10  QKKRTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAIHV------QEPNDSFNPNTFHIH 69
           +++  I+ G+  D    ++L W L  +   GD V+ +HV         + S        +
Sbjct: 9   KQRNIILVGIPIDESGVEVLKWALEEVAKHGDCVVVVHVCFTYYRALKSKSSLDRYLKPY 68

Query: 70  EDICKSKQVDLQVKVCEADSYISDLTRQVRENYATILVLGC-SLSRPDYSVFNTSLKALP 129
            + C +K+++L+ +V + +S +  L ++ +   A  +V+G    S+    +     K LP
Sbjct: 69  IEFCSTKKIELKGEVLKGNSVLGVLVKEAKRYNAMSVVVGVKQQSKLSLKIAKGCAKELP 128

Query: 130 PTCTLMVTNNLGRILLQKQGTSQQGSERTLLLSSHSSISK--------YATGYQETRISH 189
            T  ++  +  G I+ ++    Q    + +     S +S+          TG ++ +IS 
Sbjct: 129 STTDILAIHR-GNIVFRRSNHYQLPLAQKISSRPSSELSEGFSDKDLAKTTGQEKRKIS- 188

Query: 190 LHKSLDLPS---------------SLPSSSLQANENQEISLAS------KKVRSFPKLAT 249
             +SL LPS               S  ++ +  ++ ++IS+ +      ++    P    
Sbjct: 189 -GRSLSLPSVEVVDQTPGWPLLRTSTLATPMVQHQTRKISVVNWVMSLPERFPHHPNQTC 248

Query: 250 GELFPKLALQD-SKGIKRHFTYEQLKCATNNFDPEMVIGEGGHSKVYKANLEDGQAVAVK 309
            + F    L+D  K I R F+Y+ LK AT++F  E +IG+GG ++VYK  LEDG+ VAVK
Sbjct: 249 QQSFCDKQLKDILKDINRWFSYDVLKTATSDFSLENLIGKGGCNEVYKGFLEDGKGVAVK 308

Query: 310 VL--SSDSSSDDLVQEVKILSEIKHENIVHIVGFCNDKNMQAIVYNL-LKGSLKQYLR-- 369
           +L  S   +  + V EV I+S + H NI  ++G C   N    VYNL  KGSL++ L+  
Sbjct: 309 ILKPSVKEAVKEFVHEVSIVSSLSHSNISPLIGVCVHYNDLISVYNLSSKGSLEETLQGK 368

Query: 370 -KLEWKERVGIAIGIAKALNYLHHSCDPPIIHRDVKSSNILLFDNFQPQLADFGEATVLN 429
             L W+ER+ IAIG+ +AL+YLH+ C  P+IHRDVKSSN+LL D F+PQL+DFG    L+
Sbjct: 369 HVLRWEERLKIAIGLGEALDYLHNQCSNPVIHRDVKSSNVLLSDEFEPQLSDFG----LS 428

Query: 430 KQSENAQSNTKPNNIVGTFGYLAPEYIMYGKIDEKIDVYSYGVVLLELITGKEAIHTDQ- 489
                +   T   ++VGTFGYLAPEY MYGK+ +K+DVY++GVVLLELI+G+ +I +D  
Sbjct: 429 MWGSKSCRYTIQRDVVGTFGYLAPEYFMYGKVSDKVDVYAFGVVLLELISGRTSISSDSP 488

Query: 490 NNHSSLVLWARSLLGRGLGDRLIDPGLK-NYEKEEMAAMMMAARLCLLFTSSRRPTMKTI 546
               SLV+WA+ ++ +G    L+DP +   +++++   M++AA  CL   ++ RP +K I
Sbjct: 489 RGQESLVMWAKPMIEKGNAKELLDPNIAGTFDEDQFHKMVLAATHCLTRAATYRPNIKEI 548

BLAST of Lsi10G008050 vs. TAIR 10
Match: AT5G63940.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 251.9 bits (642), Expect = 1.2e-66
Identity = 189/619 (30.53%), Postives = 301/619 (48.63%), Query Frame = 0

Query: 15  IIFGLKSDNGSRDILLWLLTTIVNPGDYVLAIHVQEPNDSFNPNTFH----------IHE 74
           +I G+K D  SR++L W L  +  PGD ++A+HV + +   + +             ++E
Sbjct: 22  LIVGVKPDEWSREVLTWSLVNVARPGDRIVALHVLDYSLEGSTSLISLVRNFDTMLGVYE 81

Query: 75  DICKSKQVDLQVKVCEADSYISDLTRQVRENYATILVLGCSLS----RPDYSVFNTSLKA 134
             C  KQVDL++KV    S    L ++V+   AT L++G S      R   S+     + 
Sbjct: 82  SFCNLKQVDLKLKVFRGKSARKVLVQEVKSCGATSLIVGSSRKHHTIRSSASLAKYCARN 141

Query: 135 LPPTCTLMVTNNLGRILLQKQGTSQQGSERTLLLSS---------------HSSISKYAT 194
           L    ++    + G+I+ ++   +       + + S                +S S   T
Sbjct: 142 LAKDVSVFAVKS-GKIMFRRVPNTNGADGPQMNIPSLVCGSPDIAIEAAKIGNSFSPART 201

Query: 195 GYQETRISHLHKSLDLPSSLPSSSL------QANENQEISLAS----------------- 254
             + TR S    SL  P SL   +L      Q NE    SL S                 
Sbjct: 202 SSRWTRTSR-SSSLQSPDSLGVDNLLALVPVQTNETDSGSLESGPGWHFLRGLYGNNRKS 261

Query: 255 ------KKV--------------------------------------------RSFPKLA 314
                 KK                                             R   +L 
Sbjct: 262 WTKVSAKKAVLQWVSRLRGRHSETVIYLDRKRSDSGCDEDCSSSIDGEDVSISRFGSELM 321

Query: 315 TGELFPKLAL----QDSKGIK-------RHFTYEQLKCATNNFDPEMVIGEGGHSKVYKA 374
              L P +      ++ +G+        R FTYE++   T+NF  E ++GEGG+S VY+ 
Sbjct: 322 QSPLSPFIGSNNIPEELEGLHEKYSSTCRLFTYEEVLSITSNFASENLVGEGGNSYVYRG 381

Query: 375 NLEDGQAVAVKVLSSD-SSSDDLVQEVKILSEIKHENIVHIVGFCNDKNMQAIVYNLL-K 434
           +L DG+ +AVK+L        + + E+++++ + H+NIV + GFC + N   +VY+ L +
Sbjct: 382 DLPDGRELAVKILKPCLDVLKEFILEIEVITSVHHKNIVSLFGFCFENNNLMLVYDYLPR 441

Query: 435 GSLKQYL-------RKLEWKERVGIAIGIAKALNYLHHSCDPPIIHRDVKSSNILLFDNF 494
           GSL++ L       +K  W ER  +A+G+A+AL+YLH++ DP +IHRDVKSSN+LL D+F
Sbjct: 442 GSLEENLHGNRKDAKKFGWMERYKVAVGVAEALDYLHNTHDPEVIHRDVKSSNVLLADDF 501

Query: 495 QPQLADFGEATVLNKQSENAQSNTKPNNIVGTFGYLAPEYIMYGKIDEKIDVYSYGVVLL 510
           +PQL+DFG A++ +  S++        +I GTFGYLAPEY M+GK+ +KIDVY++GVVLL
Sbjct: 502 EPQLSDFGFASLASSTSQHVAG----GDIAGTFGYLAPEYFMHGKVTDKIDVYAFGVVLL 561

BLAST of Lsi10G008050 vs. TAIR 10
Match: AT1G77280.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 244.2 bits (622), Expect = 2.5e-64
Identity = 197/677 (29.10%), Postives = 310/677 (45.79%), Query Frame = 0

Query: 13  RTIIFGLKSDNGSRDILLWLLTTIVNPGDYVLAIHV-------QEPNDSF------NPNT 72
           RTI+ G+K D  SR++L W L  +  PGD V+A+H+       +  N S         + 
Sbjct: 48  RTILVGVKLDAPSRELLTWALVKVAEPGDTVIALHILGNEIVDRAGNSSLLSLVRTFDSV 107

Query: 73  FHIHEDICKSKQVDLQVKVCEADSYISDLTRQVRENYATILVLGCSLS----RPDYSVFN 132
             ++E  C  KQVDL++K+C   S    L R+ +   AT +++G S S    R   SV  
Sbjct: 108 LDVYEGFCNLKQVDLKLKLCRGSSARKILVREAKSFSATKVLVGISKSHHAIRSSASVAK 167

Query: 133 TSLKALPPTCTLMVTNNLGRILLQKQGTSQQ------------------GSE-------- 192
              K L   C ++  NN G+IL QK+G+                     G E        
Sbjct: 168 YIAKKLSKDCWVIAVNN-GKILFQKEGSPSSTINQSQVVEETVIGFGYIGKEDVRRITLL 227

Query: 193 ----------RTLLLSSHSSISKYATGYQETRI--SHLHKSL------------------ 252
                     +T  + SHS     +   +E +    +L + L                  
Sbjct: 228 NVLQRSVTLNKTTKVVSHSEEDSNSKEEEEDQACGQNLRQVLAAARLENCSVCGFDSLSP 287

Query: 253 ---DLPSSLPSSS--------LQANENQEI---------------------------SLA 312
                P  L  +S         + N+  EI                            L 
Sbjct: 288 NDTTTPGKLSGASDFDRSEDDDECNKAMEIVPVNGSEDSGGSVTMLVRKLPEPRPGWPLL 347

Query: 313 SKKVRSFPKLATGELFP------KLALQDSK--------------------GIK------ 372
            + V +  +  T    P      KL  +D+K                    GI       
Sbjct: 348 RRAVSTLGQSVTPHKIPVVQWALKLPPRDTKQLGYDSSEDNLSTLNALVPFGINSITNKS 407

Query: 373 ------------------------RHFTYEQLKCATNNFDPEMVIGEGGHSKVYKANLED 432
                                   R F Y++L   T+NF  +  IG+GG S+V++  L +
Sbjct: 408 IPDNSPRKLPEELEGLYERFSSTCRFFKYKELVSVTSNFSADNFIGKGGSSRVFRGCLSN 467

Query: 433 GQAVAVKVL-SSDSSSDDLVQEVKILSEIKHENIVHIVGFCNDKNMQAIVYNLL-KGSLK 492
           G+ VAVK+L  ++   +D V E++I++ + H+NI+ ++GFC + +   +VYN L +GSL+
Sbjct: 468 GRVVAVKILKQTEDVLNDFVAEIEIITTLHHKNIISLLGFCFEDHNLLLVYNYLSRGSLE 527

Query: 493 QYLRKLE-------WKERVGIAIGIAKALNYLHHSCDPPIIHRDVKSSNILLFDNFQPQL 510
           + L   +       W ER  +A+G+A+AL+YLH++   P+IHRDVKSSNILL D+F+PQL
Sbjct: 528 ENLHGNKKDPLAFCWSERYKVAVGVAEALDYLHNTASQPVIHRDVKSSNILLSDDFEPQL 587

BLAST of Lsi10G008050 vs. TAIR 10
Match: AT4G35030.3 (Protein kinase superfamily protein )

HSP 1 Score: 234.2 bits (596), Expect = 2.6e-61
Identity = 152/419 (36.28%), Postives = 236/419 (56.32%), Query Frame = 0

Query: 171 RISHLHKSLDLPSSLPSSSLQANENQEISLASKKVRSFPKLATGELFPKLALQDSKGIKR 230
           ++S ++  + LP   P+   Q   N E SL  K+++               L+D+    +
Sbjct: 49  KVSVVNWVMSLPERFPNH--QQTLNYETSLIKKQIKDI-------------LRDN---NK 108

Query: 231 HFTYEQLKCATNNFDPEMVIGEGGHSKVYKANLEDGQAVAVKVLSSDS--SSDDLVQEVK 290
            F Y  L+ AT++F  E VIG+GG ++VY+  LEDG+ +AVK+L S S  +  + V E+ 
Sbjct: 109 WFNYNVLRKATSDFSQENVIGKGGCNEVYRGILEDGKGIAVKILKSSSKEAMTNFVHEIN 168

Query: 291 ILSEIKHENIVHIVGFCNDKNMQAIVYNLLK-GSLKQYLR-------KLEWKERVGIAIG 350
           I+S + H+NI  ++G C   N    VYNL   GSL++ L         L W+ER  IAIG
Sbjct: 169 IISSLSHQNISPLLGVCVQDNELISVYNLSNTGSLEETLHGKQKGKYVLSWEERFKIAIG 228

Query: 351 IAKALNYLHHSCDPPIIHRDVKSSNILLFDNFQPQLADFGEATVLNKQSENAQSN-TKPN 410
           +A+AL+YLH+ C  P+IHRDVK+SN+LL    QPQL+DFG    L+       S  +   
Sbjct: 229 LAEALDYLHNRCSKPVIHRDVKTSNVLLSLELQPQLSDFG----LSMWGPTTSSRYSIQG 288

Query: 411 NIVGTFGYLAPEYIMYGKIDEKIDVYSYGVVLLELITGKEAIH-TDQNNHSSLVLWARSL 470
           ++VGTFGYLAPEY MYGK+ +K+DVY++GVVLLELI+G+  I   +     SLV+WA+ L
Sbjct: 289 DVVGTFGYLAPEYFMYGKVSDKVDVYAFGVVLLELISGRNPISPQNPRGQESLVMWAKPL 348

Query: 471 LGRGLGDRLIDPGLKN-YEKEEMAAMMMAARLCLLFTSSRRPTMKTILRLF----EEPRY 530
           +  G    L+DP + + +++ +   M++AA  CL  +++ RP ++ ILRL     E  ++
Sbjct: 349 IDTGNLKVLLDPDVTDIFDESQFQRMVLAASHCLTRSATHRPNIRQILRLLRDENEAGKW 408

Query: 531 LLKMQK----VRDEIFNGGSTKEVADLWRSDEPDDEGNIFKVVSVYCFRKSENINLKTT 569
           +++ +       DE++   ST+   +L   +  DDE       S+    +S N    +T
Sbjct: 409 IMEEEGNEDCFDDEVYPNSSTELHLNLAMLEVEDDE-----TASISSMERSNNSLFSST 440

BLAST of Lsi10G008050 vs. TAIR 10
Match: AT1G21590.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 232.6 bits (592), Expect = 7.6e-61
Identity = 124/301 (41.20%), Postives = 194/301 (64.45%), Query Frame = 0

Query: 232 FTYEQLKCATNNFDPEMVIGEGGHSKVYKANLEDGQAVAVKVLS-SDSSSDDLVQEVKIL 291
           FTY++L   T+NF  +  IG+GG S+V++  L +G+ VAVK+L  ++    D V E+ I+
Sbjct: 397 FTYKELVSVTSNFCADNFIGKGGSSRVFRGYLPNGREVAVKILKRTECVLKDFVAEIDII 456

Query: 292 SEIKHENIVHIVGFCNDKNMQAIVYNLL-KGSLKQYLR-------KLEWKERVGIAIGIA 351
           + + H+N++ ++G+C + N   +VYN L +GSL++ L           W ER  +A+GIA
Sbjct: 457 TTLHHKNVISLLGYCFENNNLLLVYNYLSRGSLEENLHGNKKDLVAFRWNERYKVAVGIA 516

Query: 352 KALNYLHHSCDPPIIHRDVKSSNILLFDNFQPQLADFGEATVLNKQSENAQSNTKPNNIV 411
           +AL+YLH+    P+IHRDVKSSNILL D+F+PQL+DFG    L K +  + +    +++ 
Sbjct: 517 EALDYLHNDAPQPVIHRDVKSSNILLSDDFEPQLSDFG----LAKWASESTTQIICSDVA 576

Query: 412 GTFGYLAPEYIMYGKIDEKIDVYSYGVVLLELITGKEAIHTDQ-NNHSSLVLWARSLLGR 471
           GTFGYLAPEY MYGK++ KIDVY+YGVVLLEL++G++ ++++      SLV+WA+ +L  
Sbjct: 577 GTFGYLAPEYFMYGKMNNKIDVYAYGVVLLELLSGRKPVNSESPKAQDSLVMWAKPILDD 636

Query: 472 GLGDRLIDPGLK-NYEKEEMAAMMMAARLCLLFTSSRRPTMKTILRLFEEPRYLLKMQKV 522
               +L+D  L+ +   ++M  M +AA LC+      RPTM  +L L +    +LK  K+
Sbjct: 637 KEYSQLLDSSLQDDNNSDQMEKMALAATLCIRHNPQTRPTMGMVLELLKGDVEMLKWAKL 693

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FFW56.1e-5536.34Proline-rich receptor-like protein kinase PERK8 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9SGY72.3e-5442.71Putative proline-rich receptor-like protein kinase PERK11 OS=Arabidopsis thalian... [more]
Q8H1D64.0e-5439.12Receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Arabidopsis thal... [more]
Q9SX312.6e-5342.11Proline-rich receptor-like protein kinase PERK9 OS=Arabidopsis thaliana OX=3702 ... [more]
Q8RWW03.3e-5340.28Receptor-like serine/threonine-protein kinase ALE2 OS=Arabidopsis thaliana OX=37... [more]
Match NameE-valueIdentityDescription
A0A6J1CFZ74.9e-24981.14receptor-like cytosolic serine/threonine-protein kinase RBK1 OS=Momordica charan... [more]
A0A6J1I7A36.2e-22077.97PTI1-like tyrosine-protein kinase 1 OS=Cucurbita maxima OX=3661 GN=LOC111469794 ... [more]
A0A6J1G7M92.5e-21677.01PTI1-like tyrosine-protein kinase 1 OS=Cucurbita moschata OX=3662 GN=LOC11145148... [more]
A0A2P5CKG12.0e-17861.43Serine/threonine protein kinase OS=Trema orientale OX=63057 GN=TorRG33x02_281770... [more]
A0A5J5BWU53.5e-17860.47Protein kinase domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_02... [more]
Match NameE-valueIdentityDescription
XP_022140740.11.0e-24881.14receptor-like cytosolic serine/threonine-protein kinase RBK1 [Momordica charanti... [more]
XP_022970989.11.3e-21977.97PTI1-like tyrosine-protein kinase 1 [Cucurbita maxima][more]
XP_023532060.13.7e-21977.78PTI1-like tyrosine-protein kinase 2 [Cucurbita pepo subsp. pepo][more]
KAG6604957.15.4e-21877.88Alanine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosper... [more]
XP_022947700.15.1e-21677.01PTI1-like tyrosine-protein kinase 1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT2G16750.16.0e-7432.09Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
AT5G63940.11.2e-6630.53Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
AT1G77280.12.5e-6429.10Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
AT4G35030.32.6e-6136.28Protein kinase superfamily protein [more]
AT1G21590.17.6e-6141.20Protein kinase protein with adenine nucleotide alpha hydrolases-like domain [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 244..514
e-value: 3.4E-33
score: 126.3
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 247..505
e-value: 3.0E-45
score: 154.7
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 244..513
score: 36.597885
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 318..526
e-value: 3.7E-56
score: 191.7
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 220..317
e-value: 9.6E-28
score: 98.3
NoneNo IPR availablePIRSRPIRSR037921-1PIRSR037921-1coord: 250..442
e-value: 2.0E-23
score: 81.1
NoneNo IPR availablePIRSRPIRSR037568-2PIRSR037568-2coord: 235..444
e-value: 1.9E-8
score: 30.1
NoneNo IPR availablePANTHERPTHR27001OS01G0253100 PROTEINcoord: 7..533
NoneNo IPR availablePANTHERPTHR27001:SF155SERINE/THREONINE-PROTEIN KINASE CDL1-LIKE ISOFORM X1coord: 7..533
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 250..272
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 356..368
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 229..513

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G008050.1Lsi10G008050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity