Lsi10G007830 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi10G007830 vs. ExPASy Swiss-Prot
Match: Q9FN05 (Probable glucan 1,3-alpha-glucosidase OS=Arabidopsis thaliana OX=3702 GN=PSL5 PE=1 SV=1)

HSP 1 Score: 1464.9 bits (3791), Expect = 0.0e+00
Identity = 699/923 (75.73%), Postives = 787/923 (85.27%), Query Frame = 0

Query: 6   LLLLLLLALHLTFVLSWKKDEFRNCNQTPFCKRARAFKPGSCSLVAHDVSINDGDLTAKL 65
           L +L L+       LSWKK+EFR+C+QTPFCKRAR+  PG+CSL+  DVSI DGDL AKL
Sbjct: 5   LFVLSLICFCSQTALSWKKEEFRSCDQTPFCKRARSRTPGACSLIVGDVSITDGDLVAKL 64

Query: 66  LPR--NQDPDHPSKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFQVPDVILDEFLSKKL 125
           LP+  NQ      KPL+L+LSVY+DGIVRL+IDED SL PPKKRFQVPDV++ EF  KK+
Sbjct: 65  LPKAPNQGDGDQIKPLILSLSVYKDGIVRLKIDEDHSLNPPKKRFQVPDVVVSEFEEKKI 124

Query: 126 WLQRISTETIGSDLSPSSIVYLSDGYEAVLRQDPFEVFVREKSG--KRVLSLNSHGLFDF 185
           WLQ+++TETI  D SPSS+VY+SDGYEAV+R DPFEV+VREKSG  +RV+SLNSHGLFDF
Sbjct: 125 WLQKVATETISGDTSPSSVVYVSDGYEAVVRHDPFEVYVREKSGDRRRVVSLNSHGLFDF 184

Query: 186 EQLRVKEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTRGP 245
           EQL  K EG++WEEKFR HTD+RP GPQSISFDVSFYD+ FVYGIPE ATS ALKPT+GP
Sbjct: 185 EQLGRKTEGDNWEEKFRTHTDSRPSGPQSISFDVSFYDSSFVYGIPEHATSFALKPTKGP 244

Query: 246 DVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLGS 305
            VEESEPYRLFNLDVFEY HESPFG+YGSIPFM+SHGK   TSGFFWLNAAEMQIDVL +
Sbjct: 245 GVEESEPYRLFNLDVFEYDHESPFGLYGSIPFMVSHGKSGKTSGFFWLNAAEMQIDVLAN 304

Query: 306 GWDAESGISLPSSQSRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLFAT 365
           GWDAESGISLPSS SRIDTFWMSEAGIVDTFFFVGP PKDVV+QY SVTGTSAMPQLFAT
Sbjct: 305 GWDAESGISLPSSHSRIDTFWMSEAGIVDTFFFVGPEPKDVVKQYASVTGTSAMPQLFAT 364

Query: 366 AYHQCRWNYRDEEDVAQVDSKFDEHDIPYDVMWLDIEHTDGKRYFTWDRALFPNPEEMQR 425
            YHQCRWNY+DEEDVAQVDSKFDEHDIPYDV+WLDIEHTDGKRYFTWD  LFP+PEEMQ+
Sbjct: 365 GYHQCRWNYKDEEDVAQVDSKFDEHDIPYDVLWLDIEHTDGKRYFTWDSVLFPHPEEMQK 424

Query: 426 KLAAKGRHMVTIVDPHIKRDDSFPLHKEATKKGYYVKDAAGNDYDGWCWPGSSSYLDMLI 485
           KLAAKGR MVTIVDPHIKRDDS+ LHKEAT+ GYYVKD++G D+DGWCWPGSSSY+DML 
Sbjct: 425 KLAAKGRKMVTIVDPHIKRDDSYFLHKEATQMGYYVKDSSGKDFDGWCWPGSSSYIDMLS 484

Query: 486 PEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHREVHNA 545
           PEIR WWG +FS +NYVGSTPSLY WNDMNEPSVFNGPEVTMPR+ALH GGVEHREVHNA
Sbjct: 485 PEIRKWWGGRFSYKNYVGSTPSLYTWNDMNEPSVFNGPEVTMPRDALHVGGVEHREVHNA 544

Query: 546 YGYYFHMATAEGLVKRGDGKDRPFVLTRALFAGTQRYGAVWTGDNSADWDHLRVSVPMVL 605
           YGYYFHMAT++GLV R +GKDRPFVL+RA+F GTQRYGA+WTGDN+A+W+HLRVS+PM+L
Sbjct: 545 YGYYFHMATSDGLVMREEGKDRPFVLSRAIFPGTQRYGAIWTGDNTAEWEHLRVSIPMIL 604

Query: 606 TLGLTGLSFSGADVGGFFGNPETELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGERNT 665
           TLGLTG++FSGAD+GGFFGNPE ELLVRW+Q+GA+YPFFRGHAHHDTKRREPWLFGERNT
Sbjct: 605 TLGLTGITFSGADIGGFFGNPEPELLVRWYQVGAYYPFFRGHAHHDTKRREPWLFGERNT 664

Query: 666 ELMRDAIRVRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEVTPLILWQCFESTSHLR 725
           ELMRDAI  RY LLPYFYTLFREAN +G+PVVRPLWMEFP DE T     + F   S L 
Sbjct: 665 ELMRDAIHTRYTLLPYFYTLFREANVTGVPVVRPLWMEFPQDEAT-FSNDEAFMVGSGLL 724

Query: 726 SKWVYIQADEAKKVSVYLPGKQSWYDFRTGTTYRGGITHQLEVSEESIPAFQKAGTIMPR 785
            + VY +     + SVYLPGK+SWYD R G TY GG TH+++  EESIPAFQKAGTI+PR
Sbjct: 725 VQGVYTKG--TTQASVYLPGKESWYDLRNGKTYVGGKTHKMDAPEESIPAFQKAGTIIPR 784

Query: 786 KDRFRRSSTQMVNDPYTLVVALNSSQAAEGELYIDDGKSFEFKQGAFIHRRFVFSGGKLT 845
           KDRFRRSS+QM NDPYTLVVALNSSQ AEGELYIDDGKSFEF++G++IHRRFVFS G LT
Sbjct: 785 KDRFRRSSSQMDNDPYTLVVALNSSQEAEGELYIDDGKSFEFRRGSYIHRRFVFSKGVLT 844

Query: 846 SLNVGPLASSSTKFSSNCVIERIILLGH-SGSKSALVEPENRKLDIEFGPLH---FQTGQ 905
           S N+ P      + SS C+I+RIILLGH SG KSALVEP N+K +IE GPL         
Sbjct: 845 STNLAP---PEARLSSQCLIDRIILLGHSSGPKSALVEPLNQKAEIEMGPLRMGGLVASS 904

Query: 906 HVSVLTIRKPNLLITNDWTVKIL 921
              VLTIRKP + +  DWTVKIL
Sbjct: 905 GTKVLTIRKPGVRVDQDWTVKIL 921

BLAST of Lsi10G007830 vs. ExPASy Swiss-Prot
Match: B9F676 (Probable glucan 1,3-alpha-glucosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0216600 PE=3 SV=1)

HSP 1 Score: 1279.2 bits (3309), Expect = 0.0e+00
Identity = 622/925 (67.24%), Postives = 731/925 (79.03%), Query Frame = 0

Query: 7   LLLLLLALHLTFVLSWKKDEFRNCNQTPFCKRARAFKPGS----CSLVAHDVSI-NDGDL 66
           +LLLLL        +WKKDEFRNCNQTPFCKRAR   P S     SL A  +++  DG L
Sbjct: 14  VLLLLLLASSPAARAWKKDEFRNCNQTPFCKRARTRAPHSLDAPLSLDAASLAVATDGSL 73

Query: 67  TAKLLPRNQDPDHPS--KPLLLALSVYQDGIVRLRIDEDPSLG-PPKKRFQVPDVILDEF 126
           TA L        HPS  +PLLL LS      +RL+IDED S   PP +RFQVPDV+L + 
Sbjct: 74  TASL-------SHPSRLRPLLLRLSALPPHALRLQIDEDYSSNTPPHRRFQVPDVLLPDV 133

Query: 127 LSKKLWLQRISTETIGSDLSPSSIVYLSDGYEAVLRQDPFEVFVREK-SGKRVLSLNSHG 186
            ++ L L +  T   G      S   LS   + V++ DPFE+ VR   SG  VLS NSHG
Sbjct: 134 EARTLHLPQPKTSAAG-----VSTFALSSDVDVVVKHDPFELTVRRAGSGAPVLSFNSHG 193

Query: 187 LFDFEQLR-VKEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPER-ATSLAL 246
           LFDFE L+  K+EGE WEE+FR HTDTRP GPQSI+FDVSFY ADFVYG+PE  +TSLAL
Sbjct: 194 LFDFEPLQESKQEGETWEEQFRSHTDTRPRGPQSITFDVSFYGADFVYGLPEHGSTSLAL 253

Query: 247 KPTRGPDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQ 306
           +PTRGP  EESEPYRLFNLDVFEY+HESPFG+YGSIPFMI+HG    +SGFFWLNAAEMQ
Sbjct: 254 RPTRGPGAEESEPYRLFNLDVFEYLHESPFGLYGSIPFMIAHGD-GPSSGFFWLNAAEMQ 313

Query: 307 IDVLGSGWDAESGISLPSSQSRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAM 366
           IDVL  GWD  S     +   RIDT WM+EAG+VD FFFVG  PKDV++QY SVTGT +M
Sbjct: 314 IDVLAPGWDGASS----TENGRIDTLWMAEAGVVDAFFFVGSEPKDVIKQYISVTGTPSM 373

Query: 367 PQLFATAYHQCRWNYRDEEDVAQVDSKFDEHDIPYDVMWLDIEHTDGKRYFTWDRALFPN 426
           PQ FA AYHQCRWNYRDEEDVA VDS FDEHDIPYDV+WLDIEHTDGKRYFTWD + FPN
Sbjct: 374 PQQFAVAYHQCRWNYRDEEDVAGVDSGFDEHDIPYDVLWLDIEHTDGKRYFTWDHSAFPN 433

Query: 427 PEEMQRKLAAKGRHMVTIVDPHIKRDDSFPLHKEATKKGYYVKDAAGNDYDGWCWPGSSS 486
           PE MQ K+A KGR MVTIVDPHIKRD SF LH+EAT KGYYVKDA G D+DGWCWPG+SS
Sbjct: 434 PEVMQGKIADKGRKMVTIVDPHIKRDSSFHLHEEATAKGYYVKDATGKDFDGWCWPGASS 493

Query: 487 YLDMLIPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEH 546
           Y DML PEIR WW +KFS ENY GSTP+LYIWNDMNEPSVFNGPEVTMPR+A+H G VEH
Sbjct: 494 YPDMLNPEIREWWADKFSYENYKGSTPTLYIWNDMNEPSVFNGPEVTMPRDAVHYGDVEH 553

Query: 547 REVHNAYGYYFHMATAEGLVKRGDGKDRPFVLTRALFAGTQRYGAVWTGDNSADWDHLRV 606
           RE+HNAYGYYFHMATA+GL+KRG+GKDRPFVL+RA FAG+QRYGA+WTGDNSADWDHL+ 
Sbjct: 554 RELHNAYGYYFHMATADGLLKRGEGKDRPFVLSRAFFAGSQRYGAIWTGDNSADWDHLKS 613

Query: 607 SVPMVLTLGLTGLSFSGADVGGFFGNPETELLVRWFQLGAFYPFFRGHAHHDTKRREPWL 666
           S+PMVLTLGLTG++FSGAD+GGFFGNPE +LLVRW+Q+GAFYPFFRGHAHHDTKRREPWL
Sbjct: 614 SIPMVLTLGLTGMTFSGADIGGFFGNPEPDLLVRWYQVGAFYPFFRGHAHHDTKRREPWL 673

Query: 667 FGERNTELMRDAIRVRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEVTPLILWQCFE 726
           FGER T LMR+AI +RY LLPY+YTLFREA+ +G+PV+RPLW+EFP D+ T     + F 
Sbjct: 674 FGERRTALMREAIHMRYSLLPYYYTLFREASVTGVPVMRPLWLEFPDDKET-YNNGEAFM 733

Query: 727 STSHLRSKWVYIQADEAKKVSVYLPGKQSWYDFRTGTTYRGGITHQLEVSEESIPAFQKA 786
               L ++ +Y +    K VSVYLPG++ WYD R G+ Y+GG++H+LEVSE+SIP+FQ+A
Sbjct: 734 VGPSLLAQGIYEEGQ--KSVSVYLPGEELWYDLRNGSPYKGGVSHKLEVSEDSIPSFQRA 793

Query: 787 GTIMPRKDRFRRSSTQMVNDPYTLVVALNSSQAAEGELYIDDGKSFEFKQGAFIHRRFVF 846
           G I+PRKDRFRRSSTQMVNDPYTLV+ALNSS AAEGELY+DDGKS++++QGAFIHRRFVF
Sbjct: 794 GAIVPRKDRFRRSSTQMVNDPYTLVIALNSSSAAEGELYVDDGKSYDYQQGAFIHRRFVF 853

Query: 847 SGGKLTSLNVGPLASSSTKFSSNCVIERIILLG-HSGSKSALVEPENRKLDIEFGPLHFQ 906
           +  KLTS+N+ P    + KFS+ CVIERII+LG  SGSK A+VEP N ++DIE GP+  +
Sbjct: 854 ADNKLTSMNIAPKNLGNKKFSTECVIERIIILGVSSGSKKAIVEPGNHEVDIELGPISLR 913

Query: 907 TGQHVSVLTIRKPNLLITNDWTVKI 920
           +G      T+RKPN+ + +DWT++I
Sbjct: 914 SGSSSVAPTVRKPNVRVVDDWTIRI 918

BLAST of Lsi10G007830 vs. ExPASy Swiss-Prot
Match: Q94502 (Neutral alpha-glucosidase AB OS=Dictyostelium discoideum OX=44689 GN=modA PE=3 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 1.6e-235
Identity = 429/973 (44.09%), Postives = 600/973 (61.66%), Query Frame = 0

Query: 6   LLLLLLLALHLTFVLSWKKDEFRNCNQTPFCKRAR-AFKPGSCSLVAHDVSINDGDLTAK 65
           ++L ++ +L +  + S    +F+ C  + FCKR R + + G  + +    + N  + + K
Sbjct: 8   IILSIVCSLFIGSIESVDTSKFKTCKDSHFCKRNRVSHEVGVMNEMKSKQNFNIVEGSIK 67

Query: 66  LLPRN-------QDPDHPSKPLLLALSVYQDGIVRLRIDE-DPSLGPPKKRFQVPDVILD 125
           L+ +        Q+ +  S  L + L +Y+ GIVR+R  E +P L   K+R+QV DV+LD
Sbjct: 68  LVKQENTIYFDLQEQNQKSNLLTMKLEIYEGGIVRMRAQEKEPLLN--KQRYQVQDVLLD 127

Query: 126 EFLSKKL-WLQRIS--TETIGSDLSPSSIVYLSDGYEAVLRQDPFEVFVREKSGKRVLSL 185
              +  + W Q  S  + T           Y+      +++  PF++ V   + +  ++ 
Sbjct: 128 TIKTVPIQWKQEPSKQSNTFSFKHGEKECCYV------LVQLVPFKLDVYIMN-ELAITT 187

Query: 186 NSHGLFDFEQL------------RVKEEGED-------------------------WEEK 245
           NS  LF FE +            + +EE ++                         WEE+
Sbjct: 188 NSDNLFHFEPISDKPQPLPPKEKKSEEENKEANQEEDNNNNNNDNNEEQQVSTEGYWEER 247

Query: 246 FRGHTDTRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTRGPDVEESEPYRLFNLDV 305
           F  H D++P GP SI  D +F  +  VYGIPE  T L+LK T G  + E +PYRL+NLDV
Sbjct: 248 FGSHQDSKPNGPMSIGMDFTFVGSSHVYGIPEHTTRLSLKSTTGNGINE-QPYRLYNLDV 307

Query: 306 FEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLGSGWDAESGISLPSSQS 365
           FEY  +    +YG +P MISH   + T G FWLNAAE  +D+          ++ P S S
Sbjct: 308 FEYEIDKTMALYGHVPLMISH-DTKKTVGVFWLNAAETFVDI--------EDVTTPVSPS 367

Query: 366 RIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLFATAYHQCRWNYRDEEDV 425
           +  T W+SE+GI+D F+  GP P  + +QY  +TGT+A+PQ+F+  YHQC+WNY+ E+DV
Sbjct: 368 K-KTHWISESGIIDVFYLTGPTPSTIFKQYAYLTGTTALPQMFSLGYHQCKWNYKSEDDV 427

Query: 426 AQVDSKFDEHDIPYDVMWLDIEHTDGKRYFTWDRALFPNPEEMQRKLAAKGRHMVTIVDP 485
            QVD+ FDE+ IPYDV+WLDIEHTDGKRYFTWD   FP P +MQ  + AK R MVTIVDP
Sbjct: 428 KQVDNGFDENHIPYDVIWLDIEHTDGKRYFTWDNNNFPTPADMQNIIGAKHRKMVTIVDP 487

Query: 486 HIKRDDSFPLHKEATKKGYYVKDAAGNDYDGWCWPGSSSYLDMLIPEIRSWWGEKFSLEN 545
           HIKRD+++ +H EAT KGYY+K+  GNDYDGWCWPGSSSYLD   PEIR WW  +F  + 
Sbjct: 488 HIKRDNNYYVHSEATSKGYYIKNKDGNDYDGWCWPGSSSYLDFTNPEIRKWWATQFGYDK 547

Query: 546 YVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHREVHNAYGYYFHMATAEGLVK 605
           Y GSTP+LYIWNDMNEPSVFNGPEV+M ++A H GG EHR+VHN YGYY+HMA+A+GLV+
Sbjct: 548 YKGSTPNLYIWNDMNEPSVFNGPEVSMHKDAKHHGGFEHRDVHNLYGYYYHMASADGLVQ 607

Query: 606 R-GDGKDRPFVLTRALFAGTQRYGAVWTGDNSADWDHLRVSVPMVLTLGLTGLSFSGADV 665
           R  D  DRPFVL+RA +AG+QR GA+WTGDNSA W HL +S PM+L++ L G++FSGADV
Sbjct: 608 RNADQNDRPFVLSRAFYAGSQRIGAIWTGDNSAQWSHLEISNPMLLSMNLAGITFSGADV 667

Query: 666 GGFFGNPETELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGERNTELMRDAIRVRYMLL 725
           GGFFGNP+ ELL RW+Q GAF PFFRGHAH D++RREPWLF E  T ++R+AI  RY  L
Sbjct: 668 GGFFGNPDAELLTRWYQAGAFQPFFRGHAHLDSRRREPWLFNEPYTTIIREAIVKRYSYL 727

Query: 726 PYFYTLFREANTSGIPVVRPLWMEFPSDEVTPLILWQCFESTSH------LRSKWVYIQA 785
           P +YT F +   +G PV+RPLW+++P +          F+   H      L  K V  Q+
Sbjct: 728 PLWYTTFYQNTLNGAPVMRPLWVQYPKEA-------NLFDVDDHYLIGDSLLVKPVTQQS 787

Query: 786 DEAKKVSVYLPGK---QSWYDFRTGTTYRGGITHQLEVSEESIPAFQKAGTIMPRKDRFR 845
              K + V LPG+   + WYD  T      G+  +++   E IP +Q+ G+I+ +K+R R
Sbjct: 788 --CKTMKVLLPGQSVNEIWYDVDTEKPINAGVI-EIDTPLEKIPVYQRGGSIISKKERVR 847

Query: 846 RSSTQMVNDPYTLVVALNSSQAAEGELYIDDGKSFEFKQGAFIHRRFVFSGGKLTSLNVG 905
           RS+ QM +DPYT+ +AL+SS++A+G+LYIDD  SF++K+G F++R+F F    L+  +  
Sbjct: 848 RSTYQMRDDPYTIRIALDSSKSAQGQLYIDDEHSFDYKKGKFLYRQFTFKDNVLSFSDAS 907

Query: 906 PLASSSTKFSSNCVIERIILLGHSGSKSALVEPENRKLDIEFGPLHFQTGQHVSVLTIRK 920
               SST +  N  IE+I++LG     S       ++       L F+    +S LTIRK
Sbjct: 908 --NKSSTSYKPNVTIEKIVILGVQKPHSITCNITGKE------KLSFEYDSTLSKLTIRK 942

BLAST of Lsi10G007830 vs. ExPASy Swiss-Prot
Match: Q4R4N7 (Neutral alpha-glucosidase AB OS=Macaca fascicularis OX=9541 GN=GANAB PE=2 SV=1)

HSP 1 Score: 804.3 bits (2076), Expect = 1.4e-231
Identity = 428/966 (44.31%), Postives = 577/966 (59.73%), Query Frame = 0

Query: 7   LLLLLLALHLTFVLSWKKDEFRNCNQTPFCKRARAFKPGSCSLVAHDVSINDGDLTAKLL 66
           L+L+ L + L   L+  +  F+ C ++ FCKR R+ +PG   L  +   ++   L    L
Sbjct: 18  LVLVFLGVCLGITLAVDRSNFKTCEESSFCKRQRSIRPG---LSPYRALLDSLQLGPDSL 77

Query: 67  PRNQDPDHPSKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFQVPDVILDEFLSKKLWLQ 126
             + + +     L+L L   Q  + R+RIDE   L P + R++VPDV++ +    +L   
Sbjct: 78  TVHLNHEVTKVLLVLELQGLQKNMTRIRIDE---LEPRRPRYRVPDVLVADPPIARL--- 137

Query: 127 RISTETIGSDLSPSSIVYLSDGYEAVLRQDPFEVFVREKSGKRVLSLNSHGLFDFEQLRV 186
                  G D +   +      Y+ +L   PF + + E     +LS+N+ GL +FE  R 
Sbjct: 138 ----SVSGRDDNSVELTMAEGPYKIILTARPFRLDLLE-DRSLLLSVNARGLLEFEHQRA 197

Query: 187 ------------------------------------KEEGEDWEEKFRGHTDTRPYGPQS 246
                                               K+E   WEE F+ H+D++PYGP S
Sbjct: 198 PRVSQGSKDPAEGDGAQPEETPRDGDKPEETQGKAEKDEPGAWEETFKTHSDSKPYGPMS 257

Query: 247 ISFDVSFYDADFVYGIPERATSLALKPTRGPDVEESEPYRLFNLDVFEYVHESPFGIYGS 306
           +  D S    + VYGIPE A +L LK T G      EPYRL+NLDVF+Y   +P  +YGS
Sbjct: 258 VGLDFSLPGMEHVYGIPEHADNLRLKVTEG-----GEPYRLYNLDVFQYELYNPMALYGS 317

Query: 307 IPFMISHGKLRGTSGFFWLNAAEMQIDV---------LGSGWDAESGISLPSSQSRIDTF 366
           +P +++H   R   G FWLNAAE  +D+          G   D   G        + D  
Sbjct: 318 VPVLLAHNPHRDL-GIFWLNAAETWVDISSNTAGKTLFGKMMDYLQG---SGETPQTDVR 377

Query: 367 WMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLFATAYHQCRWNYRDEEDVAQVDS 426
           WMSE GI+D F  +GP   DV RQY S+TGT A+P LF+  YHQ RWNYRDE DV +VD 
Sbjct: 378 WMSETGIIDVFLLLGPSISDVFRQYASLTGTQALPPLFSLGYHQSRWNYRDEADVLEVDQ 437

Query: 427 KFDEHDIPYDVMWLDIEHTDGKRYFTWDRALFPNPEEMQRKLAAKGRHMVTIVDPHIKRD 486
            FDEH++P DV+WLDIEH DGKRYFTWD + FP P  M  +LA+K R +V IVDPHIK D
Sbjct: 438 GFDEHNLPCDVIWLDIEHADGKRYFTWDPSRFPQPRTMLERLASKRRKLVAIVDPHIKVD 497

Query: 487 DSFPLHKEATKKGYYVKDAAGNDYDGWCWPGSSSYLDMLIPEIRSWWGEKFSLENYVGST 546
             + +H E    G YVK   G+DY+GWCWPGS+ Y D   P +R+WW   FS +NY GS 
Sbjct: 498 SGYRVHDELRNLGLYVKTRDGSDYEGWCWPGSAGYPDFTNPTMRAWWANMFSYDNYEGSA 557

Query: 547 PSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHREVHNAYGYYFHMATAEGLVKRGDGK 606
           P+L++WNDMNEPSVFNGPEVTM ++A H GG EHR+VHN YG Y HMATA+GL +R  G 
Sbjct: 558 PNLFVWNDMNEPSVFNGPEVTMLKDAQHYGGWEHRDVHNIYGLYVHMATADGLRQRSGGM 617

Query: 607 DRPFVLTRALFAGTQRYGAVWTGDNSADWDHLRVSVPMVLTLGLTGLSFSGADVGGFFGN 666
           +RPFVL RA FAG+QR+GAVWTGDN+A+WDHL++S+PM L+LGL GLSF GADVGGFF N
Sbjct: 618 ERPFVLARAFFAGSQRFGAVWTGDNTAEWDHLKISIPMCLSLGLVGLSFCGADVGGFFKN 677

Query: 667 PETELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGERNTELMRDAIRVRYMLLPYFYTL 726
           PE ELLVRW+Q+GA+ PFFR HAH DT RR PWL   ++ +++RDA+  RY LLP++YTL
Sbjct: 678 PEPELLVRWYQMGAYQPFFRAHAHLDTGRRGPWLLPSQHNDIIRDALGQRYSLLPFWYTL 737

Query: 727 FREANTSGIPVVRPLWMEFPSDEVTPLILWQCFESTSHLRSKWVYIQADE-AKKVSVYLP 786
           F +A+  GIP++RPLW+++P D  T  I  Q     + L    V+  +D  A  V VYLP
Sbjct: 738 FYQAHREGIPIMRPLWVQYPQDVTTFSIDDQYLLGDALL----VHPVSDSGAHGVQVYLP 797

Query: 787 GK-QSWYDFRTGTTYRGGITHQLEVSEESIPAFQKAGTIMPRKDRFRRSSTQMVNDPYTL 846
           G+ + WYD ++   + G  T  L V+  SIP FQ+ GTI+PR  R RRSS  M +DP TL
Sbjct: 798 GQGEVWYDIQSYQKHHGPQTLYLPVTLSSIPVFQRGGTIVPRWMRVRRSSECMKDDPITL 857

Query: 847 VVALNSSQAAEGELYIDDGKSFEFK-QGAFIHRRFVFSGGKLTSLNVGPLASSSTKFSSN 906
            VAL+    AEGEL++DDG +F ++ +  F+ RRF+FSG  L S +  P       F + 
Sbjct: 858 FVALSPQGTAEGELFLDDGHTFNYETRQEFLLRRFLFSGNTLVSSSADP----EGHFETP 917

Query: 907 CVIERIILLGHSGSKSALV-----EPENRKLDIEFGPLHFQTGQHVSVLTIRKPNLLITN 920
             IER++++G +G  +A+V      PE+R        L FQ     SVL +RKP + + +
Sbjct: 918 IWIERVVIIG-AGKPAAVVLQTKGSPESR--------LSFQHDPETSVLVLRKPGINVAS 943

BLAST of Lsi10G007830 vs. ExPASy Swiss-Prot
Match: Q14697 (Neutral alpha-glucosidase AB OS=Homo sapiens OX=9606 GN=GANAB PE=1 SV=3)

HSP 1 Score: 801.2 bits (2068), Expect = 1.2e-230
Identity = 429/970 (44.23%), Postives = 575/970 (59.28%), Query Frame = 0

Query: 7   LLLLLLALHLTFVLSWKKDEFRNCNQTPFCKRARAFKPGSCSLVA--HDVSINDGDLTAK 66
           L+L  L + L   L+  +  F+ C ++ FCKR R+ +PG     A    + +    LT  
Sbjct: 18  LVLAFLGVCLGITLAVDRSNFKTCEESSFCKRQRSIRPGLSPYRALLDSLQLGPDSLTVH 77

Query: 67  LLPRNQDPDHPSKPLLLALSV--YQDGIVRLRIDEDPSLGPPKKRFQVPDVILDEFLSKK 126
           L+       H    +LL L +   Q  + R RIDE   L P + R++VPDV++ +    +
Sbjct: 78  LI-------HEVTKVLLVLELQGLQKNMTRFRIDE---LEPRRPRYRVPDVLVADPPIAR 137

Query: 127 LWLQRISTETIGSDLSPSSIVYLSDGYEAVLRQDPFEVFVREKSGKRVLSLNSHGLFDFE 186
           L          G D +   +      Y+ +L   PF + + E     +LS+N+ GL +FE
Sbjct: 138 L-------SVSGRDENSVELTMAEGPYKIILTARPFRLDLLE-DRSLLLSVNARGLLEFE 197

Query: 187 QLRV------------------------------------KEEGEDWEEKFRGHTDTRPY 246
             R                                     K+E   WEE F+ H+D++PY
Sbjct: 198 HQRAPRVSQGSKDPAEGDGAQPEETPRDGDKPEETQGKAEKDEPGAWEETFKTHSDSKPY 257

Query: 247 GPQSISFDVSFYDADFVYGIPERATSLALKPTRGPDVEESEPYRLFNLDVFEYVHESPFG 306
           GP S+  D S    + VYGIPE A +L LK T G      EPYRL+NLDVF+Y   +P  
Sbjct: 258 GPMSVGLDFSLPGMEHVYGIPEHADNLRLKVTEG-----GEPYRLYNLDVFQYELYNPMA 317

Query: 307 IYGSIPFMISHGKLRGTSGFFWLNAAEMQIDV---------LGSGWDAESGISLPSSQSR 366
           +YGS+P +++H   R   G FWLNAAE  +D+          G   D   G        +
Sbjct: 318 LYGSVPVLLAHNPHRDL-GIFWLNAAETWVDISSNTAGKTLFGKMMDYLQG---SGETPQ 377

Query: 367 IDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLFATAYHQCRWNYRDEEDVA 426
            D  WMSE GI+D F  +GP   DV RQY S+TGT A+P LF+  YHQ RWNYRDE DV 
Sbjct: 378 TDVRWMSETGIIDVFLLLGPSISDVFRQYASLTGTQALPPLFSLGYHQSRWNYRDEADVL 437

Query: 427 QVDSKFDEHDIPYDVMWLDIEHTDGKRYFTWDRALFPNPEEMQRKLAAKGRHMVTIVDPH 486
           +VD  FD+H++P DV+WLDIEH DGKRYFTWD + FP P  M  +LA+K R +V IVDPH
Sbjct: 438 EVDQGFDDHNLPCDVIWLDIEHADGKRYFTWDPSRFPQPRTMLERLASKRRKLVAIVDPH 497

Query: 487 IKRDDSFPLHKEATKKGYYVKDAAGNDYDGWCWPGSSSYLDMLIPEIRSWWGEKFSLENY 546
           IK D  + +H+E    G YVK   G+DY+GWCWPGS+ Y D   P +R+WW   FS +NY
Sbjct: 498 IKVDSGYRVHEELRNLGLYVKTRDGSDYEGWCWPGSAGYPDFTNPTMRAWWANMFSYDNY 557

Query: 547 VGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHREVHNAYGYYFHMATAEGLVKR 606
            GS P+L++WNDMNEPSVFNGPEVTM ++A H GG EHR+VHN YG Y HMATA+GL +R
Sbjct: 558 EGSAPNLFVWNDMNEPSVFNGPEVTMLKDAQHYGGWEHRDVHNIYGLYVHMATADGLRQR 617

Query: 607 GDGKDRPFVLTRALFAGTQRYGAVWTGDNSADWDHLRVSVPMVLTLGLTGLSFSGADVGG 666
             G +RPFVL RA FAG+QR+GAVWTGDN+A+WDHL++S+PM L+LGL GLSF GADVGG
Sbjct: 618 SGGMERPFVLARAFFAGSQRFGAVWTGDNTAEWDHLKISIPMCLSLGLVGLSFCGADVGG 677

Query: 667 FFGNPETELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGERNTELMRDAIRVRYMLLPY 726
           FF NPE ELLVRW+Q+GA+ PFFR HAH DT RREPWL   ++ +++RDA+  RY LLP+
Sbjct: 678 FFKNPEPELLVRWYQMGAYQPFFRAHAHLDTGRREPWLLPSQHNDIIRDALGQRYSLLPF 737

Query: 727 FYTLFREANTSGIPVVRPLWMEFPSDEVTPLILWQCFESTSHLRSKWVYIQADE-AKKVS 786
           +YTL  +A+  GIPV+RPLW+++P D  T  I  Q     + L    V+  +D  A  V 
Sbjct: 738 WYTLLYQAHREGIPVMRPLWVQYPQDVTTFNIDDQYLLGDALL----VHPVSDSGAHGVQ 797

Query: 787 VYLPGK-QSWYDFRTGTTYRGGITHQLEVSEESIPAFQKAGTIMPRKDRFRRSSTQMVND 846
           VYLPG+ + WYD ++   + G  T  L V+  SIP FQ+ GTI+PR  R RRSS  M +D
Sbjct: 798 VYLPGQGEVWYDIQSYQKHHGPQTLYLPVTLSSIPVFQRGGTIVPRWMRVRRSSECMKDD 857

Query: 847 PYTLVVALNSSQAAEGELYIDDGKSFEFK-QGAFIHRRFVFSGGKLTSLNVGPLASSSTK 906
           P TL VAL+    A+GEL++DDG +F ++ +  F+ RRF FSG  L S +  P       
Sbjct: 858 PITLFVALSPQGTAQGELFLDDGHTFNYQTRQEFLLRRFSFSGNTLVSSSADP----EGH 917

Query: 907 FSSNCVIERIILLGHSGSKSALV-----EPENRKLDIEFGPLHFQTGQHVSVLTIRKPNL 920
           F +   IER++++G +G  +A+V      PE+R        L FQ     SVL +RKP +
Sbjct: 918 FETPIWIERVVIIG-AGKPAAVVLQTKGSPESR--------LSFQHDPETSVLVLRKPGI 943

BLAST of Lsi10G007830 vs. ExPASy TrEMBL
Match: A0A6J1G7X2 (probable glucan 1,3-alpha-glucosidase OS=Cucurbita moschata OX=3662 GN=LOC111451543 PE=3 SV=1)

HSP 1 Score: 1748.0 bits (4526), Expect = 0.0e+00
Identity = 839/920 (91.20%), Postives = 873/920 (94.89%), Query Frame = 0

Query: 1   MRVPYLLLLLLLALHLTFVLSWKKDEFRNCNQTPFCKRARAFKPGSCSLVAHDVSINDGD 60
           M V YLLLLLL  LH TFVL WKK+EFR CNQTPFCKRARAFKPGSCSLVAHDVSI+DGD
Sbjct: 1   MTVSYLLLLLLFLLHFTFVLPWKKEEFRTCNQTPFCKRARAFKPGSCSLVAHDVSISDGD 60

Query: 61  LTAKLLPRNQDPDHPSKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFQVPDVILDEFLS 120
           LTA LLPRNQD ++ SKPLLLALSVYQDGI+RLRIDEDPSL PPKKRFQVPDVI+DEF +
Sbjct: 61  LTAILLPRNQDLENQSKPLLLALSVYQDGILRLRIDEDPSLAPPKKRFQVPDVIVDEFYT 120

Query: 121 KKLWLQRISTETIGSDLSPSSIVYLSDGYEAVLRQDPFEVFVREKSGKRVLSLNSHGLFD 180
           KK+WLQRISTETIG+DL PSSIVYLSD YEAVLRQDPFEVFVREKSGKRVLSLNSHGLFD
Sbjct: 121 KKIWLQRISTETIGNDLGPSSIVYLSDSYEAVLRQDPFEVFVREKSGKRVLSLNSHGLFD 180

Query: 181 FEQLRVKEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTRG 240
           FEQLRVKEEGEDWEEKFRGHTDTRPYGPQS+SFDVSFYD DFVYGIPE ATSLALKPTRG
Sbjct: 181 FEQLRVKEEGEDWEEKFRGHTDTRPYGPQSVSFDVSFYDTDFVYGIPEHATSLALKPTRG 240

Query: 241 PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG 300
           PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG
Sbjct: 241 PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG 300

Query: 301 SGWDAESGISLPSSQSRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLFA 360
           SGWDAESGISLPSSQ+RIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGT AMPQLFA
Sbjct: 301 SGWDAESGISLPSSQNRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTPAMPQLFA 360

Query: 361 TAYHQCRWNYRDEEDVAQVDSKFDEHDIPYDVMWLDIEHTDGKRYFTWDRALFPNPEEMQ 420
           TAYHQCRWNYRDEEDVA VDSKFDE+DIPYDV+WLDIEHTDGKRYFTWDRALFPNPEEMQ
Sbjct: 361 TAYHQCRWNYRDEEDVAHVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDRALFPNPEEMQ 420

Query: 421 RKLAAKGRHMVTIVDPHIKRDDSFPLHKEATKKGYYVKDAAGNDYDGWCWPGSSSYLDML 480
           RKLA KGR MVTIVDPHIKRDD+FPLHKEA+KKGYYVKDAAGND+DGWCWPGSSSYLDML
Sbjct: 421 RKLATKGRRMVTIVDPHIKRDDNFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDML 480

Query: 481 IPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHREVHN 540
            PEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHRE+HN
Sbjct: 481 SPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHRELHN 540

Query: 541 AYGYYFHMATAEGLVKRGDGKDRPFVLTRALFAGTQRYGAVWTGDNSADWDHLRVSVPMV 600
           AYGYYFHMAT+EGLVKRGDGKDRPFVL+RALFAGTQRYGAVWTGDNSADWDHLRVSVPMV
Sbjct: 541 AYGYYFHMATSEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNSADWDHLRVSVPMV 600

Query: 601 LTLGLTGLSFSGADVGGFFGNPETELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGERN 660
           LTLGLTGLSFSGADVGGFFGNPETELLVRW+QLGAFYPFFRGHAHHDTKRREPWLFGERN
Sbjct: 601 LTLGLTGLSFSGADVGGFFGNPETELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGERN 660

Query: 661 TELMRDAIRVRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEVTPLILWQCFESTSHL 720
           TELMRDAIR+RYMLLPYFYTLFREANTSG+PVVRPLWMEFPSDE T     + F   S L
Sbjct: 661 TELMRDAIRIRYMLLPYFYTLFREANTSGVPVVRPLWMEFPSDEAT-FKNDEAFMVGSGL 720

Query: 721 RSKWVYIQADEAKKVSVYLPGKQSWYDFRTGTTYRGGITHQLEVSEESIPAFQKAGTIMP 780
             + +Y +  EAK+VSVYLPGKQSWYDFRTGT Y+GGITHQLEVSEE IPAFQKAGTIMP
Sbjct: 721 LVQGIYTK--EAKRVSVYLPGKQSWYDFRTGTAYKGGITHQLEVSEEGIPAFQKAGTIMP 780

Query: 781 RKDRFRRSSTQMVNDPYTLVVALNSSQAAEGELYIDDGKSFEFKQGAFIHRRFVFSGGKL 840
           RKDRFRRSSTQMVNDPYTLVVALNSSQ AEGELYIDDGKSFEFKQGA+IHRRFVFS GKL
Sbjct: 781 RKDRFRRSSTQMVNDPYTLVVALNSSQEAEGELYIDDGKSFEFKQGAYIHRRFVFSAGKL 840

Query: 841 TSLNVGPLASSSTKFSSNCVIERIILLGHSGSKSALVEPENRKLDIEFGPLHFQTGQHVS 900
           TSLNV P AS+STKFSSNCVIERIILLGHSGSKSALVEPEN K+DIE GPLHFQTG+ +S
Sbjct: 841 TSLNVRPNASASTKFSSNCVIERIILLGHSGSKSALVEPENIKVDIELGPLHFQTGRRIS 900

Query: 901 VLTIRKPNLLITNDWTVKIL 921
           VLTIRKPNLLIT+DWTVKIL
Sbjct: 901 VLTIRKPNLLITDDWTVKIL 917

BLAST of Lsi10G007830 vs. ExPASy TrEMBL
Match: A0A6J1I0F6 (probable glucan 1,3-alpha-glucosidase OS=Cucurbita maxima OX=3661 GN=LOC111469732 PE=3 SV=1)

HSP 1 Score: 1742.2 bits (4511), Expect = 0.0e+00
Identity = 835/920 (90.76%), Postives = 871/920 (94.67%), Query Frame = 0

Query: 1   MRVPYLLLLLLLALHLTFVLSWKKDEFRNCNQTPFCKRARAFKPGSCSLVAHDVSINDGD 60
           M V YLLLLLL  LH TFVL WKK+EFR CNQTPFCKRARAFKPGSCSLVAHD SI DGD
Sbjct: 1   MTVSYLLLLLLFVLHFTFVLPWKKEEFRTCNQTPFCKRARAFKPGSCSLVAHDASITDGD 60

Query: 61  LTAKLLPRNQDPDHPSKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFQVPDVILDEFLS 120
           LTA LLPRNQD ++ SKPLLLALSVYQDGI+RLRIDEDPSL PPKKRFQVPDVI+DEF +
Sbjct: 61  LTAILLPRNQDLENQSKPLLLALSVYQDGILRLRIDEDPSLAPPKKRFQVPDVIVDEFYT 120

Query: 121 KKLWLQRISTETIGSDLSPSSIVYLSDGYEAVLRQDPFEVFVREKSGKRVLSLNSHGLFD 180
           KK+WLQRISTETIG+DLSPSSIVYLSDGYEAVLR+DPFEVFVREKSGKRVLSLNSHGLFD
Sbjct: 121 KKIWLQRISTETIGNDLSPSSIVYLSDGYEAVLRKDPFEVFVREKSGKRVLSLNSHGLFD 180

Query: 181 FEQLRVKEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTRG 240
           FEQLR+KEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPE ATSLALKPTRG
Sbjct: 181 FEQLRIKEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPEHATSLALKPTRG 240

Query: 241 PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG 300
           PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG
Sbjct: 241 PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG 300

Query: 301 SGWDAESGISLPSSQSRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLFA 360
           SGWDAESGISLPSSQ+RIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGT AMPQLFA
Sbjct: 301 SGWDAESGISLPSSQNRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTPAMPQLFA 360

Query: 361 TAYHQCRWNYRDEEDVAQVDSKFDEHDIPYDVMWLDIEHTDGKRYFTWDRALFPNPEEMQ 420
           TAYHQCRWNYRDEEDVA VDSKFDE+DIPYDV+WLDIEHTD KRYFTWDRALFPNPE+MQ
Sbjct: 361 TAYHQCRWNYRDEEDVAHVDSKFDEYDIPYDVLWLDIEHTDAKRYFTWDRALFPNPEDMQ 420

Query: 421 RKLAAKGRHMVTIVDPHIKRDDSFPLHKEATKKGYYVKDAAGNDYDGWCWPGSSSYLDML 480
           RKLA KGR MVTIVDPHIKRDD+FPLHKEA+KKGYYVKDAAGND+DGWCWPGSSSYLDML
Sbjct: 421 RKLATKGRRMVTIVDPHIKRDDNFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDML 480

Query: 481 IPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHREVHN 540
            PEIRSWWGEKFSLENY GSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHRE+HN
Sbjct: 481 SPEIRSWWGEKFSLENYAGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHRELHN 540

Query: 541 AYGYYFHMATAEGLVKRGDGKDRPFVLTRALFAGTQRYGAVWTGDNSADWDHLRVSVPMV 600
            YGYYFHMAT+EGLVKRGDGKDRPFVL+RALFAGTQRYGAVWTGDNSADWDHLRVSVPMV
Sbjct: 541 VYGYYFHMATSEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNSADWDHLRVSVPMV 600

Query: 601 LTLGLTGLSFSGADVGGFFGNPETELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGERN 660
           LTLGLTGLSFSGADVGGFFGNPETELLVRW+QLGAFYPFFRGHAHHDTKRREPWLFGERN
Sbjct: 601 LTLGLTGLSFSGADVGGFFGNPETELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGERN 660

Query: 661 TELMRDAIRVRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEVTPLILWQCFESTSHL 720
           TELMRDAIR+RYMLLPYFYTLFREAN+SG+PVVRPLWMEFPSDE T     + F   S L
Sbjct: 661 TELMRDAIRIRYMLLPYFYTLFREANSSGVPVVRPLWMEFPSDEAT-FKNDEAFMVGSGL 720

Query: 721 RSKWVYIQADEAKKVSVYLPGKQSWYDFRTGTTYRGGITHQLEVSEESIPAFQKAGTIMP 780
             + +Y +  EAK+VSVYLPGKQSWYDFRTGT Y+GGITHQLEVSEE IPAFQKAGTIMP
Sbjct: 721 LVQGIYTK--EAKRVSVYLPGKQSWYDFRTGTAYKGGITHQLEVSEEGIPAFQKAGTIMP 780

Query: 781 RKDRFRRSSTQMVNDPYTLVVALNSSQAAEGELYIDDGKSFEFKQGAFIHRRFVFSGGKL 840
           RKDRFRRSSTQMVNDPYTLVVALNSSQ AEGELYIDDGKSFEFKQGA+IHRRFVFS GKL
Sbjct: 781 RKDRFRRSSTQMVNDPYTLVVALNSSQEAEGELYIDDGKSFEFKQGAYIHRRFVFSAGKL 840

Query: 841 TSLNVGPLASSSTKFSSNCVIERIILLGHSGSKSALVEPENRKLDIEFGPLHFQTGQHVS 900
           TSLNV P AS+STKFSSNCVIERIILLGHSGSKSALVEPEN K+DIE GPLHFQTG+ +S
Sbjct: 841 TSLNVRPNASASTKFSSNCVIERIILLGHSGSKSALVEPENIKVDIELGPLHFQTGRRIS 900

Query: 901 VLTIRKPNLLITNDWTVKIL 921
           VLTIRKPNLLIT+DWTVKIL
Sbjct: 901 VLTIRKPNLLITDDWTVKIL 917

BLAST of Lsi10G007830 vs. ExPASy TrEMBL
Match: A0A1S3CCU7 (probable glucan 1,3-alpha-glucosidase OS=Cucumis melo OX=3656 GN=LOC103499585 PE=3 SV=1)

HSP 1 Score: 1740.3 bits (4506), Expect = 0.0e+00
Identity = 832/920 (90.43%), Postives = 868/920 (94.35%), Query Frame = 0

Query: 1   MRVPYLLLLLLLALHLTFVLSWKKDEFRNCNQTPFCKRARAFKPGSCSLVAHDVSINDGD 60
           MR PYLLLLLLLA HLTFVL WKKDEFRNCNQTPFCKRARAFKPGSCSLVAHDVSINDGD
Sbjct: 1   MRAPYLLLLLLLASHLTFVLPWKKDEFRNCNQTPFCKRARAFKPGSCSLVAHDVSINDGD 60

Query: 61  LTAKLLPRNQDPDHPSKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFQVPDVILDEFLS 120
           LTAKLLPRNQDPDHPSKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFQ+PDVI+DEFLS
Sbjct: 61  LTAKLLPRNQDPDHPSKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFQLPDVIVDEFLS 120

Query: 121 KKLWLQRISTETIGSDLSPSSIVYLSDGYEAVLRQDPFEVFVREKSGKRVLSLNSHGLFD 180
           KKLWLQRISTETIGSDL PSSIVYLSD YEAVLRQDPFEVFVREKSGKRVLSLNSHGLFD
Sbjct: 121 KKLWLQRISTETIGSDLRPSSIVYLSDDYEAVLRQDPFEVFVREKSGKRVLSLNSHGLFD 180

Query: 181 FEQLRVKEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTRG 240
           FEQLRVK EGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPE ATSLALKPTRG
Sbjct: 181 FEQLRVKNEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPEHATSLALKPTRG 240

Query: 241 PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG 300
           PDVEESEPYRLFNLDVFEY+++SPFG+YGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG
Sbjct: 241 PDVEESEPYRLFNLDVFEYLNDSPFGLYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG 300

Query: 301 SGWDAESGISLPSSQSRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLFA 360
           SGWDAESGISLPSSQS IDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLFA
Sbjct: 301 SGWDAESGISLPSSQSSIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLFA 360

Query: 361 TAYHQCRWNYRDEEDVAQVDSKFDEHDIPYDVMWLDIEHTDGKRYFTWDRALFPNPEEMQ 420
           TAYHQCRWNYRDEEDVA VDSKFDEHDIPYDV+WLDI+HTDGKRY TWDRALFPNPEEMQ
Sbjct: 361 TAYHQCRWNYRDEEDVAHVDSKFDEHDIPYDVLWLDIDHTDGKRYMTWDRALFPNPEEMQ 420

Query: 421 RKLAAKGRHMVTIVDPHIKRDDSFPLHKEATKKGYYVKDAAGNDYDGWCWPGSSSYLDML 480
           +KLAAKGR MVT+VDPH+KR+DSF LHKEA+K GYYVKDAAGNDYDGWCWPGSSSYLD L
Sbjct: 421 KKLAAKGRRMVTVVDPHVKREDSFTLHKEASKNGYYVKDAAGNDYDGWCWPGSSSYLDAL 480

Query: 481 IPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHREVHN 540
            PE+RSWWGEKFS +NYVGSTPSLYIWNDMNEPSVF+GPE TMPRNALH GGVEHRE+HN
Sbjct: 481 SPEVRSWWGEKFSFQNYVGSTPSLYIWNDMNEPSVFSGPEGTMPRNALHHGGVEHRELHN 540

Query: 541 AYGYYFHMATAEGLVKRGDGKDRPFVLTRALFAGTQRYGAVWTGDNSADWDHLRVSVPMV 600
           AYGYYFHMATAEGLVKRGDG DRPFVL+RA FAGTQ+YG VWTGD+SADWD LRVSVPMV
Sbjct: 541 AYGYYFHMATAEGLVKRGDGNDRPFVLSRAAFAGTQKYGTVWTGDSSADWDFLRVSVPMV 600

Query: 601 LTLGLTGLSFSGADVGGFFGNPETELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGERN 660
           LTLGLTG+SFSGADVGGFFGNPETELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGERN
Sbjct: 601 LTLGLTGVSFSGADVGGFFGNPETELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGERN 660

Query: 661 TELMRDAIRVRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEVTPLILWQCFESTSHL 720
           TELMRDAIRVRYMLLPYFYTLFREANTSGIPV+RPLWMEFPSDEVT     + F   S L
Sbjct: 661 TELMRDAIRVRYMLLPYFYTLFREANTSGIPVIRPLWMEFPSDEVT-FKNDEAFMVGSAL 720

Query: 721 RSKWVYIQADEAKKVSVYLPGKQSWYDFRTGTTYRGGITHQLEVSEESIPAFQKAGTIMP 780
             + +Y +  EAKKVSVY PGKQSWYDFRTGT Y+GG+THQ+EV EE IP FQKAGTI+P
Sbjct: 721 LVQGIYTK--EAKKVSVYFPGKQSWYDFRTGTIYKGGVTHQIEVFEEGIPTFQKAGTIIP 780

Query: 781 RKDRFRRSSTQMVNDPYTLVVALNSSQAAEGELYIDDGKSFEFKQGAFIHRRFVFSGGKL 840
           RKDRFRRSSTQMVNDPYTLVVALNSSQ AEGELYIDDGKSFEFKQGAFIHRRFVFS GKL
Sbjct: 781 RKDRFRRSSTQMVNDPYTLVVALNSSQTAEGELYIDDGKSFEFKQGAFIHRRFVFSDGKL 840

Query: 841 TSLNVGPLASSSTKFSSNCVIERIILLGHSGSKSALVEPENRKLDIEFGPLHFQTGQHVS 900
           TSLNVGP+ASSSTKFSSNC IERIILLGHSG+KSALVEPENRK+DIE GPLHF  G+  S
Sbjct: 841 TSLNVGPIASSSTKFSSNCFIERIILLGHSGAKSALVEPENRKVDIELGPLHFLKGRRTS 900

Query: 901 VLTIRKPNLLITNDWTVKIL 921
           VLTIRKPNLLI +DWTVKI+
Sbjct: 901 VLTIRKPNLLIRDDWTVKIV 917

BLAST of Lsi10G007830 vs. ExPASy TrEMBL
Match: A0A0A0LJZ9 (Gal_mutarotas_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G338840 PE=3 SV=1)

HSP 1 Score: 1727.2 bits (4472), Expect = 0.0e+00
Identity = 825/920 (89.67%), Postives = 871/920 (94.67%), Query Frame = 0

Query: 1   MRVPYLLLLLLLALHLTFVLSWKKDEFRNCNQTPFCKRARAFKPGSCSLVAHDVSINDGD 60
           MR PYLLLLLLL+LHLT VL WKKDEFRNCNQTPFCKRARAFK GSCSLVAHDVSINDGD
Sbjct: 1   MRAPYLLLLLLLSLHLTLVLPWKKDEFRNCNQTPFCKRARAFKRGSCSLVAHDVSINDGD 60

Query: 61  LTAKLLPRNQDPDHPSKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFQVPDVILDEFLS 120
           LTAKLLPRNQDPDHP  PLLL LSVYQDGIVRLRIDEDPSLGPPKKRFQ+P+VI+DEFLS
Sbjct: 61  LTAKLLPRNQDPDHPPNPLLLVLSVYQDGIVRLRIDEDPSLGPPKKRFQLPNVIVDEFLS 120

Query: 121 KKLWLQRISTETIGSDLSPSSIVYLSDGYEAVLRQDPFEVFVREKSGKRVLSLNSHGLFD 180
           +KLWLQRISTETIGSDL PSSIVYLSDGYEAVLRQDPFEVFVREKSGKRVLSLNSHGLFD
Sbjct: 121 QKLWLQRISTETIGSDLRPSSIVYLSDGYEAVLRQDPFEVFVREKSGKRVLSLNSHGLFD 180

Query: 181 FEQLRVKEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTRG 240
           FEQLRVK+EGEDWEEKFRGHTDTRP+GPQSISFDVSFYDADFVYGIPE ATSLALKPTRG
Sbjct: 181 FEQLRVKDEGEDWEEKFRGHTDTRPFGPQSISFDVSFYDADFVYGIPEHATSLALKPTRG 240

Query: 241 PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG 300
           PDVEESEPYRLFNLDVFEY+H+SPFG+YGSIPFMISHGK RGTSGFFWLNAAEMQIDVLG
Sbjct: 241 PDVEESEPYRLFNLDVFEYLHDSPFGLYGSIPFMISHGKSRGTSGFFWLNAAEMQIDVLG 300

Query: 301 SGWDAESGISLPSSQSRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLFA 360
           SGWDAESGISLPSSQS IDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLFA
Sbjct: 301 SGWDAESGISLPSSQSSIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLFA 360

Query: 361 TAYHQCRWNYRDEEDVAQVDSKFDEHDIPYDVMWLDIEHTDGKRYFTWDRALFPNPEEMQ 420
           TAYHQCRWNYRDEEDVA VDSKFDEHDIPYDV+WLDI+HTDGKRY TWDR+LFPNPEEMQ
Sbjct: 361 TAYHQCRWNYRDEEDVAHVDSKFDEHDIPYDVLWLDIDHTDGKRYMTWDRSLFPNPEEMQ 420

Query: 421 RKLAAKGRHMVTIVDPHIKRDDSFPLHKEATKKGYYVKDAAGNDYDGWCWPGSSSYLDML 480
           +KLAAKGR+MVT+VDPH+KR+DSF LHKEA+KKGYYVKDAAGNDYDGWCWPGSSSYLD L
Sbjct: 421 KKLAAKGRYMVTVVDPHVKREDSFTLHKEASKKGYYVKDAAGNDYDGWCWPGSSSYLDAL 480

Query: 481 IPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHREVHN 540
            PE+RSWWGEKFSL+NYVGSTP+LYIWNDMNEPSVF+GPE TMPRNALH GGVEHRE+HN
Sbjct: 481 SPEVRSWWGEKFSLQNYVGSTPTLYIWNDMNEPSVFSGPEGTMPRNALHYGGVEHRELHN 540

Query: 541 AYGYYFHMATAEGLVKRGDGKDRPFVLTRALFAGTQRYGAVWTGDNSADWDHLRVSVPMV 600
           AYGYYFHMAT+EGLVKRGDG DRPFVL+RA FAGTQ+YG VWTGD+SA+WD+LRVSVPMV
Sbjct: 541 AYGYYFHMATSEGLVKRGDGNDRPFVLSRAAFAGTQKYGTVWTGDSSAEWDYLRVSVPMV 600

Query: 601 LTLGLTGLSFSGADVGGFFGNPETELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGERN 660
           LTLGLTGLSFSGADVGGFFGNPE ELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGERN
Sbjct: 601 LTLGLTGLSFSGADVGGFFGNPEAELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGERN 660

Query: 661 TELMRDAIRVRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEVTPLILWQCFESTSHL 720
           TELMRDAIRVRY+LLPYFYTLFREAN +GIPVVRPLWMEFPSDEVT     + F   S L
Sbjct: 661 TELMRDAIRVRYVLLPYFYTLFREANMTGIPVVRPLWMEFPSDEVT-FKNDEAFMVGSAL 720

Query: 721 RSKWVYIQADEAKKVSVYLPGKQSWYDFRTGTTYRGGITHQLEVSEESIPAFQKAGTIMP 780
             + +Y +  EAKKVSVYLPGKQSWYDFRTGT Y+GGITHQLEV EESIP FQKAGTI+P
Sbjct: 721 LVQGIYTK--EAKKVSVYLPGKQSWYDFRTGTIYKGGITHQLEVFEESIPTFQKAGTIIP 780

Query: 781 RKDRFRRSSTQMVNDPYTLVVALNSSQAAEGELYIDDGKSFEFKQGAFIHRRFVFSGGKL 840
           RKDR RRSSTQMVNDPYTLVVALNSSQAAEGELYIDDGKSFEFKQGAFIHRRFVFS GKL
Sbjct: 781 RKDRSRRSSTQMVNDPYTLVVALNSSQAAEGELYIDDGKSFEFKQGAFIHRRFVFSDGKL 840

Query: 841 TSLNVGPLASSSTKFSSNCVIERIILLGHSGSKSALVEPENRKLDIEFGPLHFQTGQHVS 900
           TSLNVGP+ASSSTKFSSNCVIERIILLGHSG+KSALVEPENRK+DIE GPLHF  G+  S
Sbjct: 841 TSLNVGPIASSSTKFSSNCVIERIILLGHSGAKSALVEPENRKVDIELGPLHFLRGRGSS 900

Query: 901 VLTIRKPNLLITNDWTVKIL 921
           VLTIRKPNLLI++DWTVK++
Sbjct: 901 VLTIRKPNLLISDDWTVKVV 917

BLAST of Lsi10G007830 vs. ExPASy TrEMBL
Match: A0A6J1DZ48 (probable glucan 1,3-alpha-glucosidase OS=Momordica charantia OX=3673 GN=LOC111024385 PE=3 SV=1)

HSP 1 Score: 1726.8 bits (4471), Expect = 0.0e+00
Identity = 825/921 (89.58%), Postives = 871/921 (94.57%), Query Frame = 0

Query: 1   MRVPYLLLLLLLALHLTFVLSWKKDEFRNCNQTPFCKRARAFKPGSCSLVAHDVSINDGD 60
           MR+ +LLLLLL +LH TFV  WKKDEFRNCNQTPFC+RARA KPGSCSLVAHDVSINDGD
Sbjct: 1   MRISHLLLLLLFSLHSTFVFPWKKDEFRNCNQTPFCQRARALKPGSCSLVAHDVSINDGD 60

Query: 61  LTAKLLPRNQ-DPDHPSKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFQVPDVILDEFL 120
           LTAKLLPRNQ DP+H  KPLLLALSVYQDGIVRLRIDEDPSLGPPKKRF+VPDVI+DEFL
Sbjct: 61  LTAKLLPRNQDDPEHQLKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFEVPDVIVDEFL 120

Query: 121 SKKLWLQRISTETIGSDLSPSSIVYLSDGYEAVLRQDPFEVFVREKSGKRVLSLNSHGLF 180
           +KKLWLQ I+TE IGSDLSPSSIVYLSDGYEAVLR DPFEVFVREKSGKRVLSLNSHGLF
Sbjct: 121 TKKLWLQSIATEQIGSDLSPSSIVYLSDGYEAVLRHDPFEVFVREKSGKRVLSLNSHGLF 180

Query: 181 DFEQLRVKEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTR 240
           DFEQLRVKEEGEDWEEKFRGHTD RPYGPQSISFDVSFYDADFVYGIPERATSLALKPTR
Sbjct: 181 DFEQLRVKEEGEDWEEKFRGHTDKRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTR 240

Query: 241 GPDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVL 300
           GP VE+SEPYRLFNLDVFEY+HESPFG+YGSIP MISHGKLRGTSGFFWLNAAEMQIDVL
Sbjct: 241 GPGVEDSEPYRLFNLDVFEYIHESPFGLYGSIPLMISHGKLRGTSGFFWLNAAEMQIDVL 300

Query: 301 GSGWDAESGISLPSSQSRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLF 360
           GSGWDAESGISLPSSQ+RIDTFWMSEAGIVD+FFFVGPGPKDVVRQYTSVTG  AMPQLF
Sbjct: 301 GSGWDAESGISLPSSQNRIDTFWMSEAGIVDSFFFVGPGPKDVVRQYTSVTGAPAMPQLF 360

Query: 361 ATAYHQCRWNYRDEEDVAQVDSKFDEHDIPYDVMWLDIEHTDGKRYFTWDRALFPNPEEM 420
           ATAYHQCRWNYRDEEDV QVDSKFDE+DIPYDV+WLDIEHTDGKRYFTWD+ALFPNP EM
Sbjct: 361 ATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDKALFPNPVEM 420

Query: 421 QRKLAAKGRHMVTIVDPHIKRDDSFPLHKEATKKGYYVKDAAGNDYDGWCWPGSSSYLDM 480
           QRKLAAKGR MVTIVDPHIKRDDSFPLHKEA+KKGYYVKDAAGND+DGWCWPGSSSYLDM
Sbjct: 421 QRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM 480

Query: 481 LIPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHREVH 540
           L PEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPR+ALHQGGVEHRE+H
Sbjct: 481 LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRDALHQGGVEHRELH 540

Query: 541 NAYGYYFHMATAEGLVKRGDGKDRPFVLTRALFAGTQRYGAVWTGDNSADWDHLRVSVPM 600
           N YGYYFHMATAEGLVKRGDGKDRPFVL+RALFAGTQRYGAVWTGDN+ADWD LRVSVPM
Sbjct: 541 NVYGYYFHMATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNTADWDQLRVSVPM 600

Query: 601 VLTLGLTGLSFSGADVGGFFGNPETELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGER 660
           ++TLGLTGLSFSGADVGGFFGNPE ELLVRW+QLGAFYPFFRGHAHHDTKRREPWLFGER
Sbjct: 601 IVTLGLTGLSFSGADVGGFFGNPEIELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER 660

Query: 661 NTELMRDAIRVRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEVTPLILWQCFESTSH 720
           NTELMRDAIR+RYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDE T     + F   S 
Sbjct: 661 NTELMRDAIRIRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEAT-FKNDEAFMVGSA 720

Query: 721 LRSKWVYIQADEAKKVSVYLPGKQSWYDFRTGTTYRGGITHQLEVSEESIPAFQKAGTIM 780
           L  + +Y +  EAK+VSVYLPG+QSWYD RTGT YRGG+THQLEVSEESIPAFQKAGTI+
Sbjct: 721 LLVQGIYTK--EAKEVSVYLPGEQSWYDLRTGTPYRGGVTHQLEVSEESIPAFQKAGTIL 780

Query: 781 PRKDRFRRSSTQMVNDPYTLVVALNSSQAAEGELYIDDGKSFEFKQGAFIHRRFVFSGGK 840
           PRKDRFRRSSTQMVNDPYTLVVALN+SQAAEGELY+DDGKSFEFKQGA+IHRRFVFSGGK
Sbjct: 781 PRKDRFRRSSTQMVNDPYTLVVALNNSQAAEGELYVDDGKSFEFKQGAYIHRRFVFSGGK 840

Query: 841 LTSLNVGPLASSSTKFSSNCVIERIILLGHSGSKSALVEPENRKLDIEFGPLHFQTGQHV 900
           LTSLN+ P+ SSS KFSSNCVIERIILLG+SG KSALVEP+NRK+DIE GPLHFQTG+ +
Sbjct: 841 LTSLNMAPIGSSSAKFSSNCVIERIILLGYSGPKSALVEPDNRKVDIELGPLHFQTGRRI 900

Query: 901 SVLTIRKPNLLITNDWTVKIL 921
           SVLTIRKPNL IT+DWTVKIL
Sbjct: 901 SVLTIRKPNLSITDDWTVKIL 918

BLAST of Lsi10G007830 vs. NCBI nr
Match: XP_038900635.1 (probable glucan 1,3-alpha-glucosidase [Benincasa hispida])

HSP 1 Score: 1806.6 bits (4678), Expect = 0.0e+00
Identity = 870/920 (94.57%), Postives = 890/920 (96.74%), Query Frame = 0

Query: 1   MRVPYLLLLLLLALHLTFVLSWKKDEFRNCNQTPFCKRARAFKPGSCSLVAHDVSINDGD 60
           MRVPYLLLL+LLALHLTFVL WKK+EFRNCNQTPFCKRARAFKPGSCSLVAHDVSINDGD
Sbjct: 1   MRVPYLLLLILLALHLTFVLPWKKEEFRNCNQTPFCKRARAFKPGSCSLVAHDVSINDGD 60

Query: 61  LTAKLLPRNQDPDHPSKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFQVPDVILDEFLS 120
           LTAKLLPRNQDPDHP KPLLLALSVYQDGI+RLR+DEDPSLGP KKRFQVPDVILDEFLS
Sbjct: 61  LTAKLLPRNQDPDHPPKPLLLALSVYQDGILRLRVDEDPSLGPSKKRFQVPDVILDEFLS 120

Query: 121 KKLWLQRISTETIGSDLSPSSIVYLSDGYEAVLRQDPFEVFVREKSGKRVLSLNSHGLFD 180
           KKLWLQRISTETIGSDLSPSSIVYLSDGYEAVLRQDPFEVFVREKSGKR+LSLNSHGLFD
Sbjct: 121 KKLWLQRISTETIGSDLSPSSIVYLSDGYEAVLRQDPFEVFVREKSGKRILSLNSHGLFD 180

Query: 181 FEQLRVKEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTRG 240
           FEQLRVKEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTRG
Sbjct: 181 FEQLRVKEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTRG 240

Query: 241 PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG 300
           PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGK RGTSGFFWLNAAEMQIDVLG
Sbjct: 241 PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKSRGTSGFFWLNAAEMQIDVLG 300

Query: 301 SGWDAESGISLPSSQSRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLFA 360
           SGWDAESGISLPSSQSRIDT WMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLFA
Sbjct: 301 SGWDAESGISLPSSQSRIDTLWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLFA 360

Query: 361 TAYHQCRWNYRDEEDVAQVDSKFDEHDIPYDVMWLDIEHTDGKRYFTWDRALFPNPEEMQ 420
           TAYHQCRWNYRDEEDVA VDSKFDEHDIPYDV+WLDIEHTDGKRYFTWDRALFPNPEEMQ
Sbjct: 361 TAYHQCRWNYRDEEDVAHVDSKFDEHDIPYDVLWLDIEHTDGKRYFTWDRALFPNPEEMQ 420

Query: 421 RKLAAKGRHMVTIVDPHIKRDDSFPLHKEATKKGYYVKDAAGNDYDGWCWPGSSSYLDML 480
           RKLAAKGRHMVTIVDPHIKRDDSFPLHKEA+KKGYYVKDAAGNDYDGWCWPGSSSYLDML
Sbjct: 421 RKLAAKGRHMVTIVDPHIKRDDSFPLHKEASKKGYYVKDAAGNDYDGWCWPGSSSYLDML 480

Query: 481 IPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHREVHN 540
            PEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHRE+HN
Sbjct: 481 SPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHRELHN 540

Query: 541 AYGYYFHMATAEGLVKRGDGKDRPFVLTRALFAGTQRYGAVWTGDNSADWDHLRVSVPMV 600
            YGYYF MATAEGLVKRGDGKDRPFVL+RALFAGTQRYGAVWTGDNSADWDHLRVSVPMV
Sbjct: 541 VYGYYFQMATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNSADWDHLRVSVPMV 600

Query: 601 LTLGLTGLSFSGADVGGFFGNPETELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGERN 660
           LTLGLTGLSFSGADVGGFFGNPETELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGERN
Sbjct: 601 LTLGLTGLSFSGADVGGFFGNPETELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGERN 660

Query: 661 TELMRDAIRVRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEVTPLILWQCFESTSHL 720
           TELMRDAIRVRYMLLPYFYTLFREANTSGIPVVRPLW+EFPSDEVT     + F   S L
Sbjct: 661 TELMRDAIRVRYMLLPYFYTLFREANTSGIPVVRPLWLEFPSDEVT-FKNDEAFMVGSAL 720

Query: 721 RSKWVYIQADEAKKVSVYLPGKQSWYDFRTGTTYRGGITHQLEVSEESIPAFQKAGTIMP 780
             + +Y +  EAKK SVYLPGKQSWYDFRTG TYRGGITHQLEVSEESIPAFQKAGTI+P
Sbjct: 721 LVQGIYTK--EAKKASVYLPGKQSWYDFRTGATYRGGITHQLEVSEESIPAFQKAGTIIP 780

Query: 781 RKDRFRRSSTQMVNDPYTLVVALNSSQAAEGELYIDDGKSFEFKQGAFIHRRFVFSGGKL 840
           RKDRFRRSSTQMVNDPYTLVVALNSSQAAEGELYIDDGKSFEFKQGAFIHRRFVFSGGKL
Sbjct: 781 RKDRFRRSSTQMVNDPYTLVVALNSSQAAEGELYIDDGKSFEFKQGAFIHRRFVFSGGKL 840

Query: 841 TSLNVGPLASSSTKFSSNCVIERIILLGHSGSKSALVEPENRKLDIEFGPLHFQTGQHVS 900
           TSLNVGP+ASSSTKFSSNCVIERIILLGHS SKSALVEPENRK+DIE GPLHFQT +H+S
Sbjct: 841 TSLNVGPIASSSTKFSSNCVIERIILLGHSRSKSALVEPENRKVDIELGPLHFQTARHIS 900

Query: 901 VLTIRKPNLLITNDWTVKIL 921
           VLTIRKPNLLIT+DWTVKIL
Sbjct: 901 VLTIRKPNLLITDDWTVKIL 917

BLAST of Lsi10G007830 vs. NCBI nr
Match: XP_023532412.1 (probable glucan 1,3-alpha-glucosidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1751.1 bits (4534), Expect = 0.0e+00
Identity = 840/920 (91.30%), Postives = 875/920 (95.11%), Query Frame = 0

Query: 1   MRVPYLLLLLLLALHLTFVLSWKKDEFRNCNQTPFCKRARAFKPGSCSLVAHDVSINDGD 60
           M V YLLLLLL  LHLTFVL WKK+EFR CNQTPFCKRARAFKPGSCSLVAHDVSI DGD
Sbjct: 1   MTVSYLLLLLLFLLHLTFVLPWKKEEFRTCNQTPFCKRARAFKPGSCSLVAHDVSITDGD 60

Query: 61  LTAKLLPRNQDPDHPSKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFQVPDVILDEFLS 120
           LTA LLPRNQD ++ SKPLLLALSVYQDGI+RLRIDEDPSL PPKKRFQVPDVI+DEF +
Sbjct: 61  LTAILLPRNQDLENQSKPLLLALSVYQDGILRLRIDEDPSLAPPKKRFQVPDVIVDEFYT 120

Query: 121 KKLWLQRISTETIGSDLSPSSIVYLSDGYEAVLRQDPFEVFVREKSGKRVLSLNSHGLFD 180
           KK+WLQRISTETIG+DLSPSSIVYLSD YEAVLRQDPFEVFVREKSGKRVLSLNSHGLFD
Sbjct: 121 KKIWLQRISTETIGNDLSPSSIVYLSDDYEAVLRQDPFEVFVREKSGKRVLSLNSHGLFD 180

Query: 181 FEQLRVKEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTRG 240
           FEQLR+KEEGEDWEEKFRGHTDTRPYGPQS+SFDVSFYDADFVYGIPE ATSLALKPTRG
Sbjct: 181 FEQLRIKEEGEDWEEKFRGHTDTRPYGPQSVSFDVSFYDADFVYGIPEHATSLALKPTRG 240

Query: 241 PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG 300
           PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG
Sbjct: 241 PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG 300

Query: 301 SGWDAESGISLPSSQSRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLFA 360
           SGWDAESGISLPSSQ+RIDTFWMSEAGIVDTFFFVGPGPKD+VRQYTSVTGT AMPQLFA
Sbjct: 301 SGWDAESGISLPSSQNRIDTFWMSEAGIVDTFFFVGPGPKDIVRQYTSVTGTPAMPQLFA 360

Query: 361 TAYHQCRWNYRDEEDVAQVDSKFDEHDIPYDVMWLDIEHTDGKRYFTWDRALFPNPEEMQ 420
           TAYHQCRWNYRDEEDVA VDSKFDE+DIPYDV+WLDIEHTDGKRYFTWDRALFPNPEEMQ
Sbjct: 361 TAYHQCRWNYRDEEDVAHVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDRALFPNPEEMQ 420

Query: 421 RKLAAKGRHMVTIVDPHIKRDDSFPLHKEATKKGYYVKDAAGNDYDGWCWPGSSSYLDML 480
           RKLA KGR MVTIVDPHIKRDD+FPLHKEA+KKGYYVKDAAGND+DGWCWPGSSSYLDML
Sbjct: 421 RKLATKGRRMVTIVDPHIKRDDNFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDML 480

Query: 481 IPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHREVHN 540
            PEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHRE+HN
Sbjct: 481 SPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHRELHN 540

Query: 541 AYGYYFHMATAEGLVKRGDGKDRPFVLTRALFAGTQRYGAVWTGDNSADWDHLRVSVPMV 600
           AYGYYFHMAT+EGLVKRGDGKDRPFVL+RALFAGTQRYGAVWTGDNSADWDHLRVSVPMV
Sbjct: 541 AYGYYFHMATSEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNSADWDHLRVSVPMV 600

Query: 601 LTLGLTGLSFSGADVGGFFGNPETELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGERN 660
           LTLGLTGLSFSGADVGGFFGNPETELLVRW+QLGAFYPFFRGHAHHDTKRREPWLFGERN
Sbjct: 601 LTLGLTGLSFSGADVGGFFGNPETELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGERN 660

Query: 661 TELMRDAIRVRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEVTPLILWQCFESTSHL 720
           TELMRDAIR+RYMLLPYFYTLFREANTSG+PVVRPLWMEFPSDE T     + F   S L
Sbjct: 661 TELMRDAIRIRYMLLPYFYTLFREANTSGVPVVRPLWMEFPSDEAT-FKNDEAFMVGSGL 720

Query: 721 RSKWVYIQADEAKKVSVYLPGKQSWYDFRTGTTYRGGITHQLEVSEESIPAFQKAGTIMP 780
             + +Y +  EAK+VSVYLPGKQSWYDFRTGT Y+GGITHQLEVSEE IPAFQKAGTIMP
Sbjct: 721 LVQGIYTK--EAKRVSVYLPGKQSWYDFRTGTAYKGGITHQLEVSEEGIPAFQKAGTIMP 780

Query: 781 RKDRFRRSSTQMVNDPYTLVVALNSSQAAEGELYIDDGKSFEFKQGAFIHRRFVFSGGKL 840
           RKDRFRRSSTQMVNDPYTLVVALNSSQ AEGELYIDDGKSFEFKQGA+IHRRFVFS GKL
Sbjct: 781 RKDRFRRSSTQMVNDPYTLVVALNSSQEAEGELYIDDGKSFEFKQGAYIHRRFVFSAGKL 840

Query: 841 TSLNVGPLASSSTKFSSNCVIERIILLGHSGSKSALVEPENRKLDIEFGPLHFQTGQHVS 900
           TSLNV P AS+STKFSSNCVIERIILLGHSGSKSALVEPEN K+DIE GPLHFQTG+ +S
Sbjct: 841 TSLNVRPNASASTKFSSNCVIERIILLGHSGSKSALVEPENIKVDIELGPLHFQTGRRIS 900

Query: 901 VLTIRKPNLLITNDWTVKIL 921
           VLTIRKPNLLIT+DWTVKIL
Sbjct: 901 VLTIRKPNLLITDDWTVKIL 917

BLAST of Lsi10G007830 vs. NCBI nr
Match: XP_022947784.1 (probable glucan 1,3-alpha-glucosidase [Cucurbita moschata] >KAG7034833.1 putative glucan 1,3-alpha-glucosidase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1748.0 bits (4526), Expect = 0.0e+00
Identity = 839/920 (91.20%), Postives = 873/920 (94.89%), Query Frame = 0

Query: 1   MRVPYLLLLLLLALHLTFVLSWKKDEFRNCNQTPFCKRARAFKPGSCSLVAHDVSINDGD 60
           M V YLLLLLL  LH TFVL WKK+EFR CNQTPFCKRARAFKPGSCSLVAHDVSI+DGD
Sbjct: 1   MTVSYLLLLLLFLLHFTFVLPWKKEEFRTCNQTPFCKRARAFKPGSCSLVAHDVSISDGD 60

Query: 61  LTAKLLPRNQDPDHPSKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFQVPDVILDEFLS 120
           LTA LLPRNQD ++ SKPLLLALSVYQDGI+RLRIDEDPSL PPKKRFQVPDVI+DEF +
Sbjct: 61  LTAILLPRNQDLENQSKPLLLALSVYQDGILRLRIDEDPSLAPPKKRFQVPDVIVDEFYT 120

Query: 121 KKLWLQRISTETIGSDLSPSSIVYLSDGYEAVLRQDPFEVFVREKSGKRVLSLNSHGLFD 180
           KK+WLQRISTETIG+DL PSSIVYLSD YEAVLRQDPFEVFVREKSGKRVLSLNSHGLFD
Sbjct: 121 KKIWLQRISTETIGNDLGPSSIVYLSDSYEAVLRQDPFEVFVREKSGKRVLSLNSHGLFD 180

Query: 181 FEQLRVKEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTRG 240
           FEQLRVKEEGEDWEEKFRGHTDTRPYGPQS+SFDVSFYD DFVYGIPE ATSLALKPTRG
Sbjct: 181 FEQLRVKEEGEDWEEKFRGHTDTRPYGPQSVSFDVSFYDTDFVYGIPEHATSLALKPTRG 240

Query: 241 PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG 300
           PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG
Sbjct: 241 PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG 300

Query: 301 SGWDAESGISLPSSQSRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLFA 360
           SGWDAESGISLPSSQ+RIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGT AMPQLFA
Sbjct: 301 SGWDAESGISLPSSQNRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTPAMPQLFA 360

Query: 361 TAYHQCRWNYRDEEDVAQVDSKFDEHDIPYDVMWLDIEHTDGKRYFTWDRALFPNPEEMQ 420
           TAYHQCRWNYRDEEDVA VDSKFDE+DIPYDV+WLDIEHTDGKRYFTWDRALFPNPEEMQ
Sbjct: 361 TAYHQCRWNYRDEEDVAHVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDRALFPNPEEMQ 420

Query: 421 RKLAAKGRHMVTIVDPHIKRDDSFPLHKEATKKGYYVKDAAGNDYDGWCWPGSSSYLDML 480
           RKLA KGR MVTIVDPHIKRDD+FPLHKEA+KKGYYVKDAAGND+DGWCWPGSSSYLDML
Sbjct: 421 RKLATKGRRMVTIVDPHIKRDDNFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDML 480

Query: 481 IPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHREVHN 540
            PEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHRE+HN
Sbjct: 481 SPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHRELHN 540

Query: 541 AYGYYFHMATAEGLVKRGDGKDRPFVLTRALFAGTQRYGAVWTGDNSADWDHLRVSVPMV 600
           AYGYYFHMAT+EGLVKRGDGKDRPFVL+RALFAGTQRYGAVWTGDNSADWDHLRVSVPMV
Sbjct: 541 AYGYYFHMATSEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNSADWDHLRVSVPMV 600

Query: 601 LTLGLTGLSFSGADVGGFFGNPETELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGERN 660
           LTLGLTGLSFSGADVGGFFGNPETELLVRW+QLGAFYPFFRGHAHHDTKRREPWLFGERN
Sbjct: 601 LTLGLTGLSFSGADVGGFFGNPETELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGERN 660

Query: 661 TELMRDAIRVRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEVTPLILWQCFESTSHL 720
           TELMRDAIR+RYMLLPYFYTLFREANTSG+PVVRPLWMEFPSDE T     + F   S L
Sbjct: 661 TELMRDAIRIRYMLLPYFYTLFREANTSGVPVVRPLWMEFPSDEAT-FKNDEAFMVGSGL 720

Query: 721 RSKWVYIQADEAKKVSVYLPGKQSWYDFRTGTTYRGGITHQLEVSEESIPAFQKAGTIMP 780
             + +Y +  EAK+VSVYLPGKQSWYDFRTGT Y+GGITHQLEVSEE IPAFQKAGTIMP
Sbjct: 721 LVQGIYTK--EAKRVSVYLPGKQSWYDFRTGTAYKGGITHQLEVSEEGIPAFQKAGTIMP 780

Query: 781 RKDRFRRSSTQMVNDPYTLVVALNSSQAAEGELYIDDGKSFEFKQGAFIHRRFVFSGGKL 840
           RKDRFRRSSTQMVNDPYTLVVALNSSQ AEGELYIDDGKSFEFKQGA+IHRRFVFS GKL
Sbjct: 781 RKDRFRRSSTQMVNDPYTLVVALNSSQEAEGELYIDDGKSFEFKQGAYIHRRFVFSAGKL 840

Query: 841 TSLNVGPLASSSTKFSSNCVIERIILLGHSGSKSALVEPENRKLDIEFGPLHFQTGQHVS 900
           TSLNV P AS+STKFSSNCVIERIILLGHSGSKSALVEPEN K+DIE GPLHFQTG+ +S
Sbjct: 841 TSLNVRPNASASTKFSSNCVIERIILLGHSGSKSALVEPENIKVDIELGPLHFQTGRRIS 900

Query: 901 VLTIRKPNLLITNDWTVKIL 921
           VLTIRKPNLLIT+DWTVKIL
Sbjct: 901 VLTIRKPNLLITDDWTVKIL 917

BLAST of Lsi10G007830 vs. NCBI nr
Match: KAG6604702.1 (putative glucan 1,3-alpha-glucosidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1744.6 bits (4517), Expect = 0.0e+00
Identity = 837/919 (91.08%), Postives = 871/919 (94.78%), Query Frame = 0

Query: 1   MRVPYLLLLLLLALHLTFVLSWKKDEFRNCNQTPFCKRARAFKPGSCSLVAHDVSINDGD 60
           M V YLLLLLL  LH TFVL WKK+EFR CNQTPFCKRARAFKPGSCSLVAHDVSI+DGD
Sbjct: 1   MTVSYLLLLLLFLLHFTFVLPWKKEEFRTCNQTPFCKRARAFKPGSCSLVAHDVSISDGD 60

Query: 61  LTAKLLPRNQDPDHPSKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFQVPDVILDEFLS 120
           LTA LLPRNQD ++ SKPLLLALSVYQDGI+RLRIDEDPSL PPKKRFQVPDVI+DEF +
Sbjct: 61  LTAILLPRNQDLENQSKPLLLALSVYQDGILRLRIDEDPSLAPPKKRFQVPDVIVDEFYT 120

Query: 121 KKLWLQRISTETIGSDLSPSSIVYLSDGYEAVLRQDPFEVFVREKSGKRVLSLNSHGLFD 180
           KK+WLQRISTETIG+DL PSSIVYLSD YEAVLRQDPFEVFVREKSGKRVLSLNSHGLFD
Sbjct: 121 KKIWLQRISTETIGNDLGPSSIVYLSDSYEAVLRQDPFEVFVREKSGKRVLSLNSHGLFD 180

Query: 181 FEQLRVKEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTRG 240
           FEQLRVKEEGEDWEEKFRGHTDTRPYGPQS+SFDVSFYD DFVYGIPE ATSLALKPTRG
Sbjct: 181 FEQLRVKEEGEDWEEKFRGHTDTRPYGPQSVSFDVSFYDTDFVYGIPEHATSLALKPTRG 240

Query: 241 PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG 300
           PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG
Sbjct: 241 PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG 300

Query: 301 SGWDAESGISLPSSQSRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLFA 360
           SGWDAESGISLPSSQ+RIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGT AMPQLFA
Sbjct: 301 SGWDAESGISLPSSQNRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTPAMPQLFA 360

Query: 361 TAYHQCRWNYRDEEDVAQVDSKFDEHDIPYDVMWLDIEHTDGKRYFTWDRALFPNPEEMQ 420
           TAYHQCRWNYRDEEDVA VDSKFDE+DIPYDV+WLDIEHTDGKRYFTWDRALFPNPEEMQ
Sbjct: 361 TAYHQCRWNYRDEEDVAHVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDRALFPNPEEMQ 420

Query: 421 RKLAAKGRHMVTIVDPHIKRDDSFPLHKEATKKGYYVKDAAGNDYDGWCWPGSSSYLDML 480
           RKLA KGR MVTIVDPHIKRDD+FPLHKEA+KKGYYVKDAAGND+DGWCWPGSSSYLDML
Sbjct: 421 RKLATKGRRMVTIVDPHIKRDDNFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDML 480

Query: 481 IPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHREVHN 540
            PEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHRE+HN
Sbjct: 481 SPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHRELHN 540

Query: 541 AYGYYFHMATAEGLVKRGDGKDRPFVLTRALFAGTQRYGAVWTGDNSADWDHLRVSVPMV 600
           AYGYYFHMAT+EGLVKRGDGKDRPFVL+RALFAGTQRYGAVWTGDNSADWDHLRVSVPMV
Sbjct: 541 AYGYYFHMATSEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNSADWDHLRVSVPMV 600

Query: 601 LTLGLTGLSFSGADVGGFFGNPETELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGERN 660
           LTLGLTGLSFSGADVGGFFGNPETELLVRW+QLGAFYPFFRGHAHHDTKRREPWLFGERN
Sbjct: 601 LTLGLTGLSFSGADVGGFFGNPETELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGERN 660

Query: 661 TELMRDAIRVRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEVTPLILWQCFESTSHL 720
           TELMRDAIR+RYMLLPYFYTLFREANTSG+PVVRPLWMEFPSDE T     + F   S L
Sbjct: 661 TELMRDAIRIRYMLLPYFYTLFREANTSGVPVVRPLWMEFPSDEAT-FKNDEAFMVGSGL 720

Query: 721 RSKWVYIQADEAKKVSVYLPGKQSWYDFRTGTTYRGGITHQLEVSEESIPAFQKAGTIMP 780
             + +Y +  EAK+VSVYLPGKQSWYDFRTGT Y+GGITHQLEVSEE IPAFQKAGTIMP
Sbjct: 721 LVQGIYTK--EAKRVSVYLPGKQSWYDFRTGTAYKGGITHQLEVSEEGIPAFQKAGTIMP 780

Query: 781 RKDRFRRSSTQMVNDPYTLVVALNSSQAAEGELYIDDGKSFEFKQGAFIHRRFVFSGGKL 840
           RKDRFRRSSTQMVNDPYTLVVALNSSQ AEGELYIDDGKSFEFKQGA+IHRRFVFS GKL
Sbjct: 781 RKDRFRRSSTQMVNDPYTLVVALNSSQEAEGELYIDDGKSFEFKQGAYIHRRFVFSAGKL 840

Query: 841 TSLNVGPLASSSTKFSSNCVIERIILLGHSGSKSALVEPENRKLDIEFGPLHFQTGQHVS 900
           TSLNV P AS+STKFSSNCVIERIILLGHSGSKSALVEPEN K+DIE GPLHFQTG+ +S
Sbjct: 841 TSLNVRPNASASTKFSSNCVIERIILLGHSGSKSALVEPENIKVDIELGPLHFQTGRRIS 900

Query: 901 VLTIRKPNLLITNDWTVKI 920
           VLTIRKPNLLIT+DWTV I
Sbjct: 901 VLTIRKPNLLITDDWTVTI 916

BLAST of Lsi10G007830 vs. NCBI nr
Match: XP_022970897.1 (probable glucan 1,3-alpha-glucosidase [Cucurbita maxima])

HSP 1 Score: 1742.2 bits (4511), Expect = 0.0e+00
Identity = 835/920 (90.76%), Postives = 871/920 (94.67%), Query Frame = 0

Query: 1   MRVPYLLLLLLLALHLTFVLSWKKDEFRNCNQTPFCKRARAFKPGSCSLVAHDVSINDGD 60
           M V YLLLLLL  LH TFVL WKK+EFR CNQTPFCKRARAFKPGSCSLVAHD SI DGD
Sbjct: 1   MTVSYLLLLLLFVLHFTFVLPWKKEEFRTCNQTPFCKRARAFKPGSCSLVAHDASITDGD 60

Query: 61  LTAKLLPRNQDPDHPSKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFQVPDVILDEFLS 120
           LTA LLPRNQD ++ SKPLLLALSVYQDGI+RLRIDEDPSL PPKKRFQVPDVI+DEF +
Sbjct: 61  LTAILLPRNQDLENQSKPLLLALSVYQDGILRLRIDEDPSLAPPKKRFQVPDVIVDEFYT 120

Query: 121 KKLWLQRISTETIGSDLSPSSIVYLSDGYEAVLRQDPFEVFVREKSGKRVLSLNSHGLFD 180
           KK+WLQRISTETIG+DLSPSSIVYLSDGYEAVLR+DPFEVFVREKSGKRVLSLNSHGLFD
Sbjct: 121 KKIWLQRISTETIGNDLSPSSIVYLSDGYEAVLRKDPFEVFVREKSGKRVLSLNSHGLFD 180

Query: 181 FEQLRVKEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTRG 240
           FEQLR+KEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPE ATSLALKPTRG
Sbjct: 181 FEQLRIKEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPEHATSLALKPTRG 240

Query: 241 PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG 300
           PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG
Sbjct: 241 PDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLG 300

Query: 301 SGWDAESGISLPSSQSRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLFA 360
           SGWDAESGISLPSSQ+RIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGT AMPQLFA
Sbjct: 301 SGWDAESGISLPSSQNRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTPAMPQLFA 360

Query: 361 TAYHQCRWNYRDEEDVAQVDSKFDEHDIPYDVMWLDIEHTDGKRYFTWDRALFPNPEEMQ 420
           TAYHQCRWNYRDEEDVA VDSKFDE+DIPYDV+WLDIEHTD KRYFTWDRALFPNPE+MQ
Sbjct: 361 TAYHQCRWNYRDEEDVAHVDSKFDEYDIPYDVLWLDIEHTDAKRYFTWDRALFPNPEDMQ 420

Query: 421 RKLAAKGRHMVTIVDPHIKRDDSFPLHKEATKKGYYVKDAAGNDYDGWCWPGSSSYLDML 480
           RKLA KGR MVTIVDPHIKRDD+FPLHKEA+KKGYYVKDAAGND+DGWCWPGSSSYLDML
Sbjct: 421 RKLATKGRRMVTIVDPHIKRDDNFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDML 480

Query: 481 IPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHREVHN 540
            PEIRSWWGEKFSLENY GSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHRE+HN
Sbjct: 481 SPEIRSWWGEKFSLENYAGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHRELHN 540

Query: 541 AYGYYFHMATAEGLVKRGDGKDRPFVLTRALFAGTQRYGAVWTGDNSADWDHLRVSVPMV 600
            YGYYFHMAT+EGLVKRGDGKDRPFVL+RALFAGTQRYGAVWTGDNSADWDHLRVSVPMV
Sbjct: 541 VYGYYFHMATSEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNSADWDHLRVSVPMV 600

Query: 601 LTLGLTGLSFSGADVGGFFGNPETELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGERN 660
           LTLGLTGLSFSGADVGGFFGNPETELLVRW+QLGAFYPFFRGHAHHDTKRREPWLFGERN
Sbjct: 601 LTLGLTGLSFSGADVGGFFGNPETELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGERN 660

Query: 661 TELMRDAIRVRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEVTPLILWQCFESTSHL 720
           TELMRDAIR+RYMLLPYFYTLFREAN+SG+PVVRPLWMEFPSDE T     + F   S L
Sbjct: 661 TELMRDAIRIRYMLLPYFYTLFREANSSGVPVVRPLWMEFPSDEAT-FKNDEAFMVGSGL 720

Query: 721 RSKWVYIQADEAKKVSVYLPGKQSWYDFRTGTTYRGGITHQLEVSEESIPAFQKAGTIMP 780
             + +Y +  EAK+VSVYLPGKQSWYDFRTGT Y+GGITHQLEVSEE IPAFQKAGTIMP
Sbjct: 721 LVQGIYTK--EAKRVSVYLPGKQSWYDFRTGTAYKGGITHQLEVSEEGIPAFQKAGTIMP 780

Query: 781 RKDRFRRSSTQMVNDPYTLVVALNSSQAAEGELYIDDGKSFEFKQGAFIHRRFVFSGGKL 840
           RKDRFRRSSTQMVNDPYTLVVALNSSQ AEGELYIDDGKSFEFKQGA+IHRRFVFS GKL
Sbjct: 781 RKDRFRRSSTQMVNDPYTLVVALNSSQEAEGELYIDDGKSFEFKQGAYIHRRFVFSAGKL 840

Query: 841 TSLNVGPLASSSTKFSSNCVIERIILLGHSGSKSALVEPENRKLDIEFGPLHFQTGQHVS 900
           TSLNV P AS+STKFSSNCVIERIILLGHSGSKSALVEPEN K+DIE GPLHFQTG+ +S
Sbjct: 841 TSLNVRPNASASTKFSSNCVIERIILLGHSGSKSALVEPENIKVDIELGPLHFQTGRRIS 900

Query: 901 VLTIRKPNLLITNDWTVKIL 921
           VLTIRKPNLLIT+DWTVKIL
Sbjct: 901 VLTIRKPNLLITDDWTVKIL 917

BLAST of Lsi10G007830 vs. TAIR 10
Match: AT5G63840.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 1464.9 bits (3791), Expect = 0.0e+00
Identity = 699/923 (75.73%), Postives = 787/923 (85.27%), Query Frame = 0

Query: 6   LLLLLLLALHLTFVLSWKKDEFRNCNQTPFCKRARAFKPGSCSLVAHDVSINDGDLTAKL 65
           L +L L+       LSWKK+EFR+C+QTPFCKRAR+  PG+CSL+  DVSI DGDL AKL
Sbjct: 5   LFVLSLICFCSQTALSWKKEEFRSCDQTPFCKRARSRTPGACSLIVGDVSITDGDLVAKL 64

Query: 66  LPR--NQDPDHPSKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFQVPDVILDEFLSKKL 125
           LP+  NQ      KPL+L+LSVY+DGIVRL+IDED SL PPKKRFQVPDV++ EF  KK+
Sbjct: 65  LPKAPNQGDGDQIKPLILSLSVYKDGIVRLKIDEDHSLNPPKKRFQVPDVVVSEFEEKKI 124

Query: 126 WLQRISTETIGSDLSPSSIVYLSDGYEAVLRQDPFEVFVREKSG--KRVLSLNSHGLFDF 185
           WLQ+++TETI  D SPSS+VY+SDGYEAV+R DPFEV+VREKSG  +RV+SLNSHGLFDF
Sbjct: 125 WLQKVATETISGDTSPSSVVYVSDGYEAVVRHDPFEVYVREKSGDRRRVVSLNSHGLFDF 184

Query: 186 EQLRVKEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTRGP 245
           EQL  K EG++WEEKFR HTD+RP GPQSISFDVSFYD+ FVYGIPE ATS ALKPT+GP
Sbjct: 185 EQLGRKTEGDNWEEKFRTHTDSRPSGPQSISFDVSFYDSSFVYGIPEHATSFALKPTKGP 244

Query: 246 DVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVLGS 305
            VEESEPYRLFNLDVFEY HESPFG+YGSIPFM+SHGK   TSGFFWLNAAEMQIDVL +
Sbjct: 245 GVEESEPYRLFNLDVFEYDHESPFGLYGSIPFMVSHGKSGKTSGFFWLNAAEMQIDVLAN 304

Query: 306 GWDAESGISLPSSQSRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLFAT 365
           GWDAESGISLPSS SRIDTFWMSEAGIVDTFFFVGP PKDVV+QY SVTGTSAMPQLFAT
Sbjct: 305 GWDAESGISLPSSHSRIDTFWMSEAGIVDTFFFVGPEPKDVVKQYASVTGTSAMPQLFAT 364

Query: 366 AYHQCRWNYRDEEDVAQVDSKFDEHDIPYDVMWLDIEHTDGKRYFTWDRALFPNPEEMQR 425
            YHQCRWNY+DEEDVAQVDSKFDEHDIPYDV+WLDIEHTDGKRYFTWD  LFP+PEEMQ+
Sbjct: 365 GYHQCRWNYKDEEDVAQVDSKFDEHDIPYDVLWLDIEHTDGKRYFTWDSVLFPHPEEMQK 424

Query: 426 KLAAKGRHMVTIVDPHIKRDDSFPLHKEATKKGYYVKDAAGNDYDGWCWPGSSSYLDMLI 485
           KLAAKGR MVTIVDPHIKRDDS+ LHKEAT+ GYYVKD++G D+DGWCWPGSSSY+DML 
Sbjct: 425 KLAAKGRKMVTIVDPHIKRDDSYFLHKEATQMGYYVKDSSGKDFDGWCWPGSSSYIDMLS 484

Query: 486 PEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHREVHNA 545
           PEIR WWG +FS +NYVGSTPSLY WNDMNEPSVFNGPEVTMPR+ALH GGVEHREVHNA
Sbjct: 485 PEIRKWWGGRFSYKNYVGSTPSLYTWNDMNEPSVFNGPEVTMPRDALHVGGVEHREVHNA 544

Query: 546 YGYYFHMATAEGLVKRGDGKDRPFVLTRALFAGTQRYGAVWTGDNSADWDHLRVSVPMVL 605
           YGYYFHMAT++GLV R +GKDRPFVL+RA+F GTQRYGA+WTGDN+A+W+HLRVS+PM+L
Sbjct: 545 YGYYFHMATSDGLVMREEGKDRPFVLSRAIFPGTQRYGAIWTGDNTAEWEHLRVSIPMIL 604

Query: 606 TLGLTGLSFSGADVGGFFGNPETELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGERNT 665
           TLGLTG++FSGAD+GGFFGNPE ELLVRW+Q+GA+YPFFRGHAHHDTKRREPWLFGERNT
Sbjct: 605 TLGLTGITFSGADIGGFFGNPEPELLVRWYQVGAYYPFFRGHAHHDTKRREPWLFGERNT 664

Query: 666 ELMRDAIRVRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEVTPLILWQCFESTSHLR 725
           ELMRDAI  RY LLPYFYTLFREAN +G+PVVRPLWMEFP DE T     + F   S L 
Sbjct: 665 ELMRDAIHTRYTLLPYFYTLFREANVTGVPVVRPLWMEFPQDEAT-FSNDEAFMVGSGLL 724

Query: 726 SKWVYIQADEAKKVSVYLPGKQSWYDFRTGTTYRGGITHQLEVSEESIPAFQKAGTIMPR 785
            + VY +     + SVYLPGK+SWYD R G TY GG TH+++  EESIPAFQKAGTI+PR
Sbjct: 725 VQGVYTKG--TTQASVYLPGKESWYDLRNGKTYVGGKTHKMDAPEESIPAFQKAGTIIPR 784

Query: 786 KDRFRRSSTQMVNDPYTLVVALNSSQAAEGELYIDDGKSFEFKQGAFIHRRFVFSGGKLT 845
           KDRFRRSS+QM NDPYTLVVALNSSQ AEGELYIDDGKSFEF++G++IHRRFVFS G LT
Sbjct: 785 KDRFRRSSSQMDNDPYTLVVALNSSQEAEGELYIDDGKSFEFRRGSYIHRRFVFSKGVLT 844

Query: 846 SLNVGPLASSSTKFSSNCVIERIILLGH-SGSKSALVEPENRKLDIEFGPLH---FQTGQ 905
           S N+ P      + SS C+I+RIILLGH SG KSALVEP N+K +IE GPL         
Sbjct: 845 STNLAP---PEARLSSQCLIDRIILLGHSSGPKSALVEPLNQKAEIEMGPLRMGGLVASS 904

Query: 906 HVSVLTIRKPNLLITNDWTVKIL 921
              VLTIRKP + +  DWTVKIL
Sbjct: 905 GTKVLTIRKPGVRVDQDWTVKIL 921

BLAST of Lsi10G007830 vs. TAIR 10
Match: AT3G23640.1 (heteroglycan glucosidase 1 )

HSP 1 Score: 330.5 bits (846), Expect = 4.3e-90
Identity = 182/510 (35.69%), Postives = 271/510 (53.14%), Query Frame = 0

Query: 339 PKDVVRQYTSVTGTSAMPQLFATAYHQCRWNYRDEEDVAQVDSKFDEHDIPYDVMWLDIE 398
           P  V+   +   GT  MP  +A  YHQCRW+Y  ++ VA++   F +  IP DV+W+DI+
Sbjct: 178 PTAVLESLSHAIGTVFMPPKWALGYHQCRWSYMSDKRVAEIAQTFRDKKIPSDVIWMDID 237

Query: 399 HTDGKRYFTWDRALFPNPEEMQRKLAAKGRHMVTIVDPHIKRDDSFPLHKEATKKGYYVK 458
           + DG R FT+D+  FP+P  + + L + G   + ++DP IK+++ + ++   +K   ++ 
Sbjct: 238 YMDGFRCFTFDKERFPDPSALAKDLHSNGFKAIWMLDPGIKQEEGYYVYDSGSKNDVWIS 297

Query: 459 DAAGNDYDGWCWPGSSSYLDMLIPEIRSWWGE--KFSLENYVGSTPSLYIWNDMNEPSVF 518
            A G  + G  WPG   + D    + RSWW    K  + N V       IWNDMNEP+VF
Sbjct: 298 RADGKPFTGEVWPGPCVFPDYTNSKARSWWANLVKEFVSNGVDG-----IWNDMNEPAVF 357

Query: 519 NGPEVTMPRNALHQ-----GGVE-HREVHNAYGYYFHMATAEGLVKRGDGKDRPFVLTRA 578
                TMP N +H      GGV+ H   HN YG     +T EG+ +  D   RPFVLTRA
Sbjct: 358 KVVTKTMPENNIHHGDDELGGVQNHSHYHNVYGMLMARSTYEGM-ELADKNKRPFVLTRA 417

Query: 579 LFAGTQRYGAVWTGDNSADWDHLRVSVPMVLTLGLTGLSFSGADVGGFFGNPETELLVRW 638
            F G+QRY A WTGDN ++W+HL +S+ MVL LGL+G   SG D+GGF GN    L  RW
Sbjct: 418 GFIGSQRYAATWTGDNLSNWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRW 477

Query: 639 FQLGAFYPFFRGHAHHDTKRREPWLFGERNTELMRDAIRVRYMLLPYFYTLFREANTSGI 698
             +GA +PF RGH+   T   EPW FGE   E+ R A++ RY LLP+FYTLF  A+T+G 
Sbjct: 478 MGVGAMFPFCRGHSEAGTDDHEPWSFGEECEEVCRAALKRRYQLLPHFYTLFYIAHTTGA 537

Query: 699 PVVRPLWMEFPSDEVTPLILWQCFESTSHLRSKWVY---IQADEAKKVSVYLPGKQSWYD 758
           PV  P++   P D        +  E+   L    +Y   + +  + ++   LP       
Sbjct: 538 PVAAPIFFADPIDS-----RLRAVENGFLLGPLLIYASTLSSQGSHELQHILP------- 597

Query: 759 FRTGTTYRGGITHQLEV--SEESIPA-FQKAGTIMPRKDRFRRSSTQMVNDPYTLVVALN 818
                    GI H+ +   S   +P  + + G+I+             ++D  TL+V+L+
Sbjct: 598 --------RGIWHRFDFADSHPDLPTLYLQGGSIISLAPPHLHVGEFSLSDDLTLLVSLD 657

Query: 819 SSQAAEGELYIDDGKSFEFKQGAFIHRRFV 835
            +  A+G L+ DDG  + + +G F+   ++
Sbjct: 658 ENGKAKGLLFEDDGDGYGYTKGRFLVTHYI 661

BLAST of Lsi10G007830 vs. TAIR 10
Match: AT3G23640.2 (heteroglycan glucosidase 1 )

HSP 1 Score: 330.5 bits (846), Expect = 4.3e-90
Identity = 182/510 (35.69%), Postives = 271/510 (53.14%), Query Frame = 0

Query: 339 PKDVVRQYTSVTGTSAMPQLFATAYHQCRWNYRDEEDVAQVDSKFDEHDIPYDVMWLDIE 398
           P  V+   +   GT  MP  +A  YHQCRW+Y  ++ VA++   F +  IP DV+W+DI+
Sbjct: 178 PTAVLESLSHAIGTVFMPPKWALGYHQCRWSYMSDKRVAEIAQTFRDKKIPSDVIWMDID 237

Query: 399 HTDGKRYFTWDRALFPNPEEMQRKLAAKGRHMVTIVDPHIKRDDSFPLHKEATKKGYYVK 458
           + DG R FT+D+  FP+P  + + L + G   + ++DP IK+++ + ++   +K   ++ 
Sbjct: 238 YMDGFRCFTFDKERFPDPSALAKDLHSNGFKAIWMLDPGIKQEEGYYVYDSGSKNDVWIS 297

Query: 459 DAAGNDYDGWCWPGSSSYLDMLIPEIRSWWGE--KFSLENYVGSTPSLYIWNDMNEPSVF 518
            A G  + G  WPG   + D    + RSWW    K  + N V       IWNDMNEP+VF
Sbjct: 298 RADGKPFTGEVWPGPCVFPDYTNSKARSWWANLVKEFVSNGVDG-----IWNDMNEPAVF 357

Query: 519 NGPEVTMPRNALHQ-----GGVE-HREVHNAYGYYFHMATAEGLVKRGDGKDRPFVLTRA 578
                TMP N +H      GGV+ H   HN YG     +T EG+ +  D   RPFVLTRA
Sbjct: 358 KVVTKTMPENNIHHGDDELGGVQNHSHYHNVYGMLMARSTYEGM-ELADKNKRPFVLTRA 417

Query: 579 LFAGTQRYGAVWTGDNSADWDHLRVSVPMVLTLGLTGLSFSGADVGGFFGNPETELLVRW 638
            F G+QRY A WTGDN ++W+HL +S+ MVL LGL+G   SG D+GGF GN    L  RW
Sbjct: 418 GFIGSQRYAATWTGDNLSNWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRW 477

Query: 639 FQLGAFYPFFRGHAHHDTKRREPWLFGERNTELMRDAIRVRYMLLPYFYTLFREANTSGI 698
             +GA +PF RGH+   T   EPW FGE   E+ R A++ RY LLP+FYTLF  A+T+G 
Sbjct: 478 MGVGAMFPFCRGHSEAGTDDHEPWSFGEECEEVCRAALKRRYQLLPHFYTLFYIAHTTGA 537

Query: 699 PVVRPLWMEFPSDEVTPLILWQCFESTSHLRSKWVY---IQADEAKKVSVYLPGKQSWYD 758
           PV  P++   P D        +  E+   L    +Y   + +  + ++   LP       
Sbjct: 538 PVAAPIFFADPIDS-----RLRAVENGFLLGPLLIYASTLSSQGSHELQHILP------- 597

Query: 759 FRTGTTYRGGITHQLEV--SEESIPA-FQKAGTIMPRKDRFRRSSTQMVNDPYTLVVALN 818
                    GI H+ +   S   +P  + + G+I+             ++D  TL+V+L+
Sbjct: 598 --------RGIWHRFDFADSHPDLPTLYLQGGSIISLAPPHLHVGEFSLSDDLTLLVSLD 657

Query: 819 SSQAAEGELYIDDGKSFEFKQGAFIHRRFV 835
            +  A+G L+ DDG  + + +G F+   ++
Sbjct: 658 ENGKAKGLLFEDDGDGYGYTKGRFLVTHYI 661

BLAST of Lsi10G007830 vs. TAIR 10
Match: AT3G45940.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 281.2 bits (718), Expect = 3.0e-75
Identity = 232/843 (27.52%), Postives = 371/843 (44.01%), Query Frame = 0

Query: 49  LVAHDVSINDGDLTAKLLPRNQDPDHPSKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRF 108
           L++ + S +DG     L  +  +  + S   +L L +      RLR+    +    K+R+
Sbjct: 30  LISMEKSPDDGSFIGYLQVKQSNKIYGSDITILRLFINYRTDHRLRVHITDA---KKQRW 89

Query: 109 QVP-DVILDEFLSKKLWLQRISTETIGSDLSPSSIVYLSDGYEAVLRQDPFEVFVREKSG 168
           +VP +++  E     +   R S  T+     P  I+        +   DPF   VR +S 
Sbjct: 90  EVPYNLLRREQPPNVIGKSRKSPVTVQEISGPELIL--------IFTVDPFSFAVRRRSN 149

Query: 169 KRVLSLNSHGLFDFEQLRVKEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIP 228
              +   S     F ++  K++  +                 S+  D S Y     +G  
Sbjct: 150 GETIFNTSSSDESFGEMVFKDQYLEIS--------------TSLPKDASLYG----FGEN 209

Query: 229 ERATSLALKPTRGPDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFF 288
            +A  + L P        +EPY LF  DV  +   +   +YGS P  +    LR  SG  
Sbjct: 210 SQANGIKLVP--------NEPYTLFTEDVSAFNLNT--DLYGSHPVYMD---LRNVSGKA 269

Query: 289 WLNAAEMQIDVLGSGWDAESGISLPSSQSRIDTFWMSEA-------GIVDTFFFVGPGPK 348
           + ++                   L  +   +D F+  ++       G+ D +FF GP P 
Sbjct: 270 YAHSV------------------LLLNSHGMDVFYRGDSLTYKVIGGVFDFYFFAGPSPL 329

Query: 349 DVVRQYTSVTGTSAMPQLFATAYHQCRWNYRDEEDVAQVDSKFDEHDIPYDVMWLDIEHT 408
           +VV QYTS+ G  A    ++  +HQCRW YR+   V  V   + +  IP DV+W D ++ 
Sbjct: 330 NVVDQYTSLIGRPAPMPYWSLGFHQCRWGYRNVSVVKDVVDNYQKAKIPLDVIWNDADYM 389

Query: 409 DGKRYFTWDRALFPNPEEMQ--RKLAAKGRHMVTIVDPHIKRDDSFPLHKEATKKGYYVK 468
           DG + FT D   FP+ + +    ++   G   V I DP I  + S+ +++       ++K
Sbjct: 390 DGYKDFTLDLVNFPHAKLLSFLDRIHKMGMKYVVIKDPGIGVNASYGVYQRGMASDVFIK 449

Query: 469 DAAGNDYDGWCWPGSSSYLDMLIPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNG 528
              G  +    WPG   + D L P+  SWWG++  +  +    P   +W DMNE +    
Sbjct: 450 -YEGKPFLAQVWPGPVYFPDFLNPKTVSWWGDE--IRRFHELVPIDGLWIDMNEINATGH 509

Query: 529 PE----VTMPRNALHQGGVEHREVHNAYGYYFHMATAEGLVKRGDGKDRPFVLTRALFAG 588
                  T+P +A H  GV   + H+ YG+   +AT + L+    GK RPF+L+R+ F G
Sbjct: 510 KASLGFKTIPTSAYHYNGVREYDAHSIYGFSEAIATHKALL-AVQGK-RPFILSRSTFVG 569

Query: 589 TQRYGAVWTGDNSADWDHLRVSVPMVLTLGLTGLSFSGADVGGFFGNPETELLVRWFQLG 648
           + +Y A WTGDN   W  L+VS+  +L  G+ G+   G+D+ GFF     EL  RW ++G
Sbjct: 570 SGQYAAHWTGDNQGTWQSLQVSISTMLNFGIFGVPMVGSDICGFFPPTPEELCNRWIEVG 629

Query: 649 AFYPFFRGHAHHDTKRREPWLFGERNTELMRDAIRVRYMLLPYFYTLFREANTSGIPVVR 708
           AFYPF R HA +   R+E + +G    E  R+A+ +RY LLP+ YTL  EA+ SG P+ R
Sbjct: 630 AFYPFSRDHADYYAPRKELYQWG-TVAESARNALGMRYKLLPFLYTLNYEAHMSGAPIAR 689

Query: 709 PLWMEFPSDEVTPLILWQCFESTSHLRSKWVYIQADEAKKVSVYLPGKQSWY---DFRTG 768
           PL+  FP       +  Q    +S + S    ++  + +  +++ PG  SWY   D    
Sbjct: 690 PLFFSFPEFTECYGLSKQFLLGSSLMISP--VLEQGKTQVEALFPPG--SWYHMFDMTQV 749

Query: 769 TTYRGGITHQLEVSEESIPAFQKAGTIMPRKDRFRRSSTQMVNDPYTLVVALNSSQAAEG 828
              + G    L      +        I+P +        Q+V  P     A  S   A G
Sbjct: 750 VVSKNGRLFTLPAPFNVVNVHLYQNAILPMQ--------QVVAFP-----AGASEGYASG 789

Query: 829 ELYIDDGKSFEFK--QGAFIHRRFVFSGGKLTSLNVGPLASSSTKFSSNCVIERIILLGH 873
           +L++DD +  E K   G   +  F  S G  +      +       S   VIE++I+LG 
Sbjct: 810 KLFLDDDELPEMKLGNGKSTYIDFYASVGNESVKIWSQVKEGQFALSQGLVIEKVIVLGL 789

BLAST of Lsi10G007830 vs. TAIR 10
Match: AT5G11720.1 (Glycosyl hydrolases family 31 protein )

HSP 1 Score: 265.4 bits (677), Expect = 1.7e-70
Identity = 188/618 (30.42%), Postives = 287/618 (46.44%), Query Frame = 0

Query: 247 EPYRLFNLDVFEYVHESP-FGIYGSIPFMISHGKLRG------TSGFFWLNAAEMQIDVL 306
           E   L+N D+     E+P   +YGS PF +     +G      T G   LN+  M +   
Sbjct: 211 ETMTLWNADIGS---ENPDVNLYGSHPFYMDVRGSKGNEEAGTTHGVLLLNSNGMDVKYE 270

Query: 307 GSGWDAESGISLPSSQSRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLF 366
           G                RI   +    G++D + F GP P+ V+ QYT + G  A    +
Sbjct: 271 G---------------HRIT--YNVIGGVIDLYVFAGPSPEMVMNQYTELIGRPAPMPYW 330

Query: 367 ATAYHQCRWNYRDEEDVAQVDSKFDEHDIPYDVMWLDIEHTDGKRYFTWDRALFPNPEEM 426
           +  +HQCR+ Y++  D+  V   + +  IP +VMW DI++ DG + FT D   FP  ++M
Sbjct: 331 SFGFHQCRYGYKNVSDLEYVVDGYAKAGIPLEVMWTDIDYMDGYKDFTLDPVNFPE-DKM 390

Query: 427 QR---KLAAKGRHMVTIVDPHIKRDDSFPLHKEATKKGYYVKDAAGNDYDGWCWPGSSSY 486
           Q     L   G+  V I+DP I  D S+  +    +   ++K   G  Y G  WPG   +
Sbjct: 391 QSFVDTLHKNGQKYVLILDPGIGVDSSYGTYNRGMEADVFIK-RNGEPYLGEVWPGKVYF 450

Query: 487 LDMLIPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVF------NGPEV--------- 546
            D L P   ++W  +  +   +   P   +W DMNE S F      +G  +         
Sbjct: 451 PDFLNPAAATFWSNEIKMFQEI--LPLDGLWIDMNELSNFITSPLSSGSSLDDPPYKINN 510

Query: 547 ----------TMPRNALHQGGVEHREVHNAYGYYFHMATAEGLVKRGDGKDRPFVLTRAL 606
                     T+P  ++H G +   + HN YG     AT + +V    GK RPF+L+R+ 
Sbjct: 511 SGDKRPINNKTVPATSIHFGNISEYDAHNLYGLLEAKATHQAVVDI-TGK-RPFILSRST 570

Query: 607 FAGTQRYGAVWTGDNSADWDHLRVSVPMVLTLGLTGLSFSGADVGGFFGNPETELLVRWF 666
           F  + +Y A WTGDN+A W+ L  S+P +L  GL G+   GAD+ GF  +   EL  RW 
Sbjct: 571 FVSSGKYTAHWTGDNAAKWEDLAYSIPGILNFGLFGIPMVGADICGFSHDTTEELCRRWI 630

Query: 667 QLGAFYPFFRGHAHHDTKRREPWLFGERNTELMRDAIRVRYMLLPYFYTLFREANTSGIP 726
           QLGAFYPF R H+   T R+E +L+ +      R  + +R  LLP+ YTL  EA+ SG P
Sbjct: 631 QLGAFYPFARDHSSLGTARQELYLW-DSVASSARKVLGLRMRLLPHLYTLMYEAHVSGNP 690

Query: 727 VVRPLWMEFPSDEVTPLILWQCFESTSHLRSKWVYIQADEAKKVSVYLPGKQSWYDFRTG 786
           + RPL+  FP D  T  I  Q     S + S  +      A  V  Y P   +W+D    
Sbjct: 691 IARPLFFSFPQDTKTYEIDSQFLIGKSIMVSPAL---KQGAVAVDAYFPA-GNWFDLFNY 750

Query: 787 TTYRGGITH---QLEVSEESIPAFQKAGTIMPRKDRFRRSSTQMVNDPYTLVVALNSSQA 827
           +   GG +    +L+   + +    + G+I+  +     ++      PY L+V  +  + 
Sbjct: 751 SFAVGGDSGKHVRLDTPADHVNVHVREGSIVAMQGE-ALTTRDARKTPYQLLVVASRLEN 796