Lsi10G007270 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi10G007270
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	1,3-beta-glucan synthase
Location	chr10: 10109282 .. 10111941 (-)
RNA-Seq Expression	Lsi10G007270
Synteny	Lsi10G007270

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAACAGGGAGATGGATCTCTTGCTAGTTCCTTATTCATCAGATCCCAGCTTAAAAATAATCCAATGGCCTCCCTTTTTACTAGCCAGCAAGGTCGATACGGTCCCTTATGTTTTCTTTTATGTATATCTTACCTTTTCAGTTTTGGGCATACTTGTAGCGACTGTTAACAACTTCTCTACTGCTACAGATTCCAATAGCATTGGATATGGCTGCTGAATTTCGATCCAAAGACTCTGACCTCTGGAAACGTATTTGTGCAGATGAGTACATGAAATGTGCAGTGATAGAGTGTTATGAATCTTTCAAAAATGTCCTGAATGTTCTGGTGGTTGGAGAAAATGAGAAAAGGTTCTAAGCTACAGTCCTACTTATTGAACCAAACATCATCAAATGATCATAGTTTTCCCTCCCTACAAAGAGAAAACATGCAAAAGCTTAACCTGTAGTTCTATATCATTTGTAAAACTAAGTATTTCATATGAAGGGGAAATTGCAGCCTTGGGAATTTTATAAACAATAAATCATCATTTACTTCATTTTCTCTCACTGCCTTAAGAGGAAAAAACTATTAATGTTTTGTAATGTCAACTAATTTCATCTATGGCTGTTTATGGGTCACCTGTAAATGTTTTTTGAGTACCAATCATCCAGGGTTGAAATAATGCATAAACTGCAATTATGTTTCTGCAGGATGCTATAACTAAACGGAGAAATGAGAGCCTATGTCAATTGTTTTCGTGAATTCTAACTCCATTTGACTTTTACAGGATTATTGGGACCATTATTAAAGAAGTTGAAAATAATATTGCAAAGAACACACTTCTAACAAACTTCAAAATGGGTCCCTTACTCAGTTTGTGCCAGAAATTTGTGGAACTCGTGGAGATCTTGGTAAGTTATGATGCAATTCTGAAGTATCTGTTGGTCTATTATTTCAGATTCGAAACTTTGCTCTTGTTTCCTATTTCAGAAAGATGGAGACCCATCTAAGCAAGATACAGTGGTGCTTTTGCTGCAAGATATGTTAGAAGTTGTCACTCGAGATATGATGCACAACGAAGTCCGGTTAGCAAATATAATTTGTGCAATTTGAAGTAAATTTATTTATGTCTACTGGCATCCTTCAAATGGTTACTGTTATATGCTACAATGGTAACTTGTTATATCTTTCAGTGAATTGGCAGAACTTGGTCATAACAAGGACTCTGGAAGGCAACTCTTTGCTGGTACTGACACTAAACCTGCTATAAACTTTCCTCCTTCAGTTACAGCTCAATGGGAAGAGCAGGTGGGCATGTTGAAAATAAATAATAGTACTTTCGAGAAATTATCTATCATTCTAAGATTTTAACTGATTAATTGAAATAACACAATCTTGTTTTTGTGGGTGTATGTCAGATTAAACGCCTCTATCTACTATTGACTGTAAAAGAATCTGCCACTGAGGTTCCCATAAATCTCGAAGCACGAAGAAGAATTGCATTTTTCACAAATTCATTGTTTATGGATATGCCACGTGCTCCACGAGTCCGTAAGATGCTATCATTCAGGTCTGGGTTTTCTTTAACTCTATATGAGCAGAAAGCATATGTCTCTTCTATTTGGATAAGTTTTTTTTTCCAAATTTGAGATTGGATGCATCACCCTTTCTCTAGAGTATGCTTGCCCCTGCGGCTAATCTAAGATTAAAGATCTGAGCAAACTAAAATAAGTTATAAATGTTTTGGCACCTTTATTAAGCACACATTCATGTTTCATGTCTTATAACTGCAGGAATGGATTTTTCTTTTAAGCTTTGATATAAAAATTAGAGATTTTATTCTACCTCTTGGCACTTCCAATAAAAATTACTTTTCGGTTTTTCTTAAGAAACATCTTTCGTTAAAAATTAAATGTTACTAATATACCTCAGTTCCTGTACTTGGTAATATTTTTGGGTTATTGACAGCATTGTGTCTCCAAAATATAGTGAAAAGATGGGTCTGTTCTAATACTGACCTTGAGATGAATATTTTTGGGTTGTTCACAGTGTTATGACTCCATACTACAGTGAAGAGACTGTCTATTCTAAAACTGACCTTGAGATGGAAAATGAGGATGGTGTCTCCATCATATATTACTTGCAAAAGATCTTTCCAGGTGAGTGATATATAGAAACGACACAAATATCTCTTGGTTCTTCGATAAGTGATTTCTGGGTGACTTATCTCATGAACATTTGCCTTCTTGCTAGATGAATGGAACAACTTCATGGAGCGACTCAATTGTAAAAAAGACAGTGAGATTTGGGAAAATGAAGAAAATATATTACACCTCCGCCATTGGGCGTCCTTACGAGGACAAACTCTATCAAGAACAGGTAGCTTAAACTCATTACATTTACTTTCAAGTTCATTTATGCATCTCCATTGCTGGCATTAACACAATTATTACAGTTAGGGGTATGATGTATTATCGAAGGGCTTTGAAGCTTCAGGCATTTCTTGACATGGCTTCTGAAAGTGGTAAGCATGTGCATGTTAACATTCTCTAGTAATTTGTAATCAAATTTACTTCTCAATAATGCTTGTTTTTTATTCCTTCACTCTTTGTGTGGTTGAGATGTCTTGTTTCCTTGTCAGCACTGTTCGTGTGCTTTCTCTCTAATGAGTATTAA

mRNA sequence

ATGAAACAGGGAGATGGATCTCTTGCTAGTTCCTTATTCATCAGATCCCAGCTTAAAAATAATCCAATGGCCTCCCTTTTTACTAGCCAGCAAGGTCGATACGGTCCCTTATGTTTTCTTTTATATGAGTACATGAAATGTGCAGTGATAGAGTGTTATGAATCTTTCAAAAATGTCCTGAATGTTCTGGTGGTTGGAGAAAATGAGAAAAGGATTATTGGGACCATTATTAAAGAAGTTGAAAATAATATTGCAAAGAACACACTTCTAACAAACTTCAAAATGGGTCCCTTACTCAGTTTGTGCCAGAAATTTGTGGAACTCGTGGAGATCTTGAAAGATGGAGACCCATCTAAGCAAGATACAGTGGTGCTTTTGCTGCAAGATATGTTAGAAGTTGTCACTCGAGATATGATGCACAACGAAGTCCGTGAATTGGCAGAACTTGGTCATAACAAGGACTCTGGAAGGCAACTCTTTGCTGGTACTGACACTAAACCTGCTATAAACTTTCCTCCTTCAGTTACAGCTCAATGGGAAGAGCAGATTAAACGCCTCTATCTACTATTGACTGTAAAAGAATCTGCCACTGAGGTTCCCATAAATCTCGAAGCACGAAGAAGAATTGCATTTTTCACAAATTCATTGTTTATGGATATGCCACGTGCTCCACGAGTCCGTAAGATGCTATCATTCAGTGTTATGACTCCATACTACAGTGAAGAGACTGTCTATTCTAAAACTGACCTTGAGATGGAAAATGAGGATGGTGTCTCCATCATATATTACTTGCAAAAGATCTTTCCAGGTGAGGGTATGATGTATTATCGAAGGGCTTTGAAGCTTCAGGCATTTCTTGACATGGCTTCTGAAAGTGCACTGTTCGTGTGCTTTCTCTCTAATGAGTATTAA

Coding sequence (CDS)

ATGAAACAGGGAGATGGATCTCTTGCTAGTTCCTTATTCATCAGATCCCAGCTTAAAAATAATCCAATGGCCTCCCTTTTTACTAGCCAGCAAGGTCGATACGGTCCCTTATGTTTTCTTTTATATGAGTACATGAAATGTGCAGTGATAGAGTGTTATGAATCTTTCAAAAATGTCCTGAATGTTCTGGTGGTTGGAGAAAATGAGAAAAGGATTATTGGGACCATTATTAAAGAAGTTGAAAATAATATTGCAAAGAACACACTTCTAACAAACTTCAAAATGGGTCCCTTACTCAGTTTGTGCCAGAAATTTGTGGAACTCGTGGAGATCTTGAAAGATGGAGACCCATCTAAGCAAGATACAGTGGTGCTTTTGCTGCAAGATATGTTAGAAGTTGTCACTCGAGATATGATGCACAACGAAGTCCGTGAATTGGCAGAACTTGGTCATAACAAGGACTCTGGAAGGCAACTCTTTGCTGGTACTGACACTAAACCTGCTATAAACTTTCCTCCTTCAGTTACAGCTCAATGGGAAGAGCAGATTAAACGCCTCTATCTACTATTGACTGTAAAAGAATCTGCCACTGAGGTTCCCATAAATCTCGAAGCACGAAGAAGAATTGCATTTTTCACAAATTCATTGTTTATGGATATGCCACGTGCTCCACGAGTCCGTAAGATGCTATCATTCAGTGTTATGACTCCATACTACAGTGAAGAGACTGTCTATTCTAAAACTGACCTTGAGATGGAAAATGAGGATGGTGTCTCCATCATATATTACTTGCAAAAGATCTTTCCAGGTGAGGGTATGATGTATTATCGAAGGGCTTTGAAGCTTCAGGCATTTCTTGACATGGCTTCTGAAAGTGCACTGTTCGTGTGCTTTCTCTCTAATGAGTATTAA

Protein sequence

MKQGDGSLASSLFIRSQLKNNPMASLFTSQQGRYGPLCFLLYEYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIAKNTLLTNFKMGPLLSLCQKFVELVEILKDGDPSKQDTVVLLLQDMLEVVTRDMMHNEVRELAELGHNKDSGRQLFAGTDTKPAINFPPSVTAQWEEQIKRLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPRAPRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPGEGMMYYRRALKLQAFLDMASESALFVCFLSNEY

Homology

[Close]

BLAST of Lsi10G007270 vs. ExPASy Swiss-Prot
Match: Q3B724 (Callose synthase 5 OS=Arabidopsis thaliana OX=3702 GN=CALS5 PE=1 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 2.1e-107
Identity = 207/295 (70.17%), Postives = 229/295 (77.63%), Query Frame = 0


Query: 43   EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIAKNTLLTNFKMGPLLSLC 102
            EYMKCAVIECYESFK+VL+ LV+GENEKRIIG IIKEVE+NI+KN+ L+NF+M PL +LC
Sbjct: 883  EYMKCAVIECYESFKHVLHTLVIGENEKRIIGIIIKEVESNISKNSFLSNFRMAPLPALC 942

Query: 103  QKFVELVEILKDGDPSKQDTVVLLLQDMLEVVTRDMMHNEVRELAELGH-NKDSGRQLFA 162
             KFVELV ILK+ DP+K+DTVVLLLQDMLEVVTRDMM NE REL ELGH NK+SGRQLFA
Sbjct: 943  SKFVELVGILKNADPAKRDTVVLLLQDMLEVVTRDMMQNENRELVELGHTNKESGRQLFA 1002

Query: 163  GTDTKPAINFPPSVTAQWEEQIKRLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMP 222
            GTD KPAI FPP  TAQW EQI RL+LLLTVKESA +VP NLEA+RRIAFFTNSLFMDMP
Sbjct: 1003 GTDAKPAILFPPVATAQWHEQISRLHLLLTVKESAMDVPTNLEAQRRIAFFTNSLFMDMP 1062

Query: 223  RAPRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPGE---------- 282
            RAPRVR MLSFSV+TPYYSEETVYSK DLEMENEDGVS++YYLQKIFP E          
Sbjct: 1063 RAPRVRNMLSFSVLTPYYSEETVYSKNDLEMENEDGVSVVYYLQKIFPDEWTNFLERLDC 1122

Query: 283  --------------------------------GMMYYRRALKLQAFLDMASESAL 295
                                            GMMYYRRALKLQAFLDMA+E+ +
Sbjct: 1123 KDETSVLESEENILQLRHWVSLRGQTLFRTVRGMMYYRRALKLQAFLDMANETEI 1177

[Close]

BLAST of Lsi10G007270 vs. ExPASy Swiss-Prot
Match: Q9LXT9 (Callose synthase 3 OS=Arabidopsis thaliana OX=3702 GN=CALS3 PE=3 SV=3)

HSP 1 Score: 244.2 bits (622), Expect = 1.9e-63
Identity = 146/305 (47.87%), Postives = 180/305 (59.02%), Query Frame = 0


Query: 44   YMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIAKNTLLTNFKMGPLLSLCQ 103
            YMKCAV ECY SFKN++  +V G  EK +I  I  EV+ +I    L+  +KM  L SL  
Sbjct: 893  YMKCAVRECYASFKNIIKFVVQGNREKEVIEIIFAEVDKHIDTGDLIQEYKMSALPSLYD 952

Query: 104  KFVELVEILKDGDPSKQDTVVLLLQDMLEVVTRDMMHNE--VRELAELGHN--------- 163
             FV+L++ L D     +D VV+L QDMLEVVTRD+M  +  +  L +  H          
Sbjct: 953  HFVKLIKYLLDNKEEDRDHVVILFQDMLEVVTRDIMMEDYNISSLVDSSHGGTWHGGMIP 1012

Query: 164  KDSGRQLFAGTDTKPAINFP-PSVTAQWEEQIKRLYLLLTVKESATEVPINLEARRRIAF 223
             +   QLFA +    AI FP   VT  W+E+IKR+YLLLT KESA +VP NLEARRRI+F
Sbjct: 1013 LEQQYQLFASSG---AIRFPIEPVTEAWKEKIKRIYLLLTTKESAMDVPSNLEARRRISF 1072

Query: 224  FTNSLFMDMPRAPRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPGE 283
            F+NSLFMDMP AP+VR MLSFSV+TPYY+EE ++S  DLE  NEDGVSI++YLQKIFP E
Sbjct: 1073 FSNSLFMDMPMAPKVRNMLSFSVLTPYYTEEVLFSLRDLETPNEDGVSILFYLQKIFPDE 1132

Query: 284  ------------------------------------------GMMYYRRALKLQAFLDMA 295
                                                      GMMYYR+AL+LQAFLDMA
Sbjct: 1133 WNNFLERVKCLSEEELKESDELEEELRLWASYRGQTLTRTVRGMMYYRKALELQAFLDMA 1192

[Close]

BLAST of Lsi10G007270 vs. ExPASy Swiss-Prot
Match: Q9AUE0 (Callose synthase 1 OS=Arabidopsis thaliana OX=3702 GN=CALS1 PE=1 SV=2)

HSP 1 Score: 241.5 bits (615), Expect = 1.2e-62
Identity = 139/299 (46.49%), Postives = 179/299 (59.87%), Query Frame = 0


Query: 44   YMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIAKNTLLTNFKMGPLLSLCQ 103
            YM CAV ECY SFKN++N LVVGE E ++I  I  +++ +I K TL+T   +  L  L  
Sbjct: 887  YMTCAVRECYASFKNLINYLVVGEREGQVINDIFSKIDEHIEKETLITELNLSALPDLYG 946

Query: 104  KFVELVEILKDGDPSKQDTVVLLLQDMLEVVTRDMMHNEVRELAELGHN-----KDSGRQ 163
            +FV L+E L +     +D +V++L +MLE+VTRD+M  EV  L E  HN      D    
Sbjct: 947  QFVRLIEYLLENREEDKDQIVIVLLNMLELVTRDIMEEEVPSLLETAHNGSYVKYDVMTP 1006

Query: 164  LFAGTDTKPAINFPP-SVTAQWEEQIKRLYLLLTVKESATEVPINLEARRRIAFFTNSLF 223
            L         + FP  S T  W+E+IKRL+LLLTVKESA +VP NLEARRR+ FF+NSLF
Sbjct: 1007 LHQQRKYFSQLRFPVYSQTEAWKEKIKRLHLLLTVKESAMDVPSNLEARRRLTFFSNSLF 1066

Query: 224  MDMPRAPRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPGE------ 283
            MDMP AP++R MLSFSV+TPY+SE+ ++S   LE +NEDGVSI++YLQKIFP E      
Sbjct: 1067 MDMPPAPKIRNMLSFSVLTPYFSEDVLFSIFGLEQQNEDGVSILFYLQKIFPDEWTNFLE 1126

Query: 284  ------------------------------------GMMYYRRALKLQAFLDMASESAL 295
                                                GMMYYR+AL+LQAFLDMA +  L
Sbjct: 1127 RVKCGNEEELRAREDLEEELRLWASYRGQTLTKTVRGMMYYRKALELQAFLDMAKDEEL 1185

[Close]

BLAST of Lsi10G007270 vs. ExPASy Swiss-Prot
Match: Q9SL03 (Callose synthase 2 OS=Arabidopsis thaliana OX=3702 GN=CALS2 PE=2 SV=3)

HSP 1 Score: 238.0 bits (606), Expect = 1.4e-61
Identity = 138/299 (46.15%), Postives = 178/299 (59.53%), Query Frame = 0


Query: 44   YMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIAKNTLLTNFKMGPLLSLCQ 103
            YM CAV ECY SFKN++N LVVGE E ++I  I   ++ +I K TL+ +  +  L  L  
Sbjct: 887  YMTCAVRECYASFKNLINFLVVGEREGQVINEIFSRIDEHIEKETLIKDLNLSALPDLYG 946

Query: 104  KFVELVEILKDGDPSKQDTVVLLLQDMLEVVTRDMMHNEVRELAELGHN-----KDSGRQ 163
            +FV L+E L +     +D +V++L +MLEVVTRD+M  EV  + E  HN      D    
Sbjct: 947  QFVRLIEYLMENREEDKDQIVIVLLNMLEVVTRDIMDEEVPSMLESTHNGTYVKYDVMTP 1006

Query: 164  LFAGTDTKPAINFPP-SVTAQWEEQIKRLYLLLTVKESATEVPINLEARRRIAFFTNSLF 223
            L         + FP  S T  W+E+IKRL+LLLTVKESA +VP NLEARRR+ FF+NSLF
Sbjct: 1007 LHQQRKYFSQLRFPVYSQTEAWKEKIKRLHLLLTVKESAMDVPSNLEARRRLTFFSNSLF 1066

Query: 224  MDMPRAPRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPGE------ 283
            M+MP AP++R MLSFSV+TPYYSE+ ++S   LE +NEDGVSI++YLQKIFP E      
Sbjct: 1067 MEMPDAPKIRNMLSFSVLTPYYSEDVLFSIFGLEKQNEDGVSILFYLQKIFPDEWTNFLE 1126

Query: 284  ------------------------------------GMMYYRRALKLQAFLDMASESAL 295
                                                GMMYYR+AL+LQAFLDMA +  L
Sbjct: 1127 RVKCGSEEELRAREELEEELRLWASYRGQTLTKTVRGMMYYRKALELQAFLDMAKDEEL 1185

[Close]

BLAST of Lsi10G007270 vs. ExPASy Swiss-Prot
Match: Q9LUD7 (Putative callose synthase 8 OS=Arabidopsis thaliana OX=3702 GN=CALS8 PE=3 SV=2)

HSP 1 Score: 201.8 bits (512), Expect = 1.1e-50
Identity = 131/310 (42.26%), Postives = 170/310 (54.84%), Query Frame = 0


Query: 43   EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIAKNTLLTNFKMGPLLSLC 102
            EYM  AV ECYES K +L +LVVG+ EK+II  II E+E +I +++LL  FKM  L +L 
Sbjct: 931  EYMYYAVKECYESLKYILQILVVGDLEKKIISGIINEIEESIRQSSLLEEFKMAELPALH 990

Query: 103  QKFVELVEILKDGDPSK---------QDTVVLLLQDMLEVVTRDMMHNEVRELAELGHNK 162
             K +ELV++L +G   +            +V  LQD+ E+VT DMM +  R L  L   +
Sbjct: 991  DKCIELVQLLVEGSAEQLQVEKSEELHGKLVKALQDIFELVTNDMMVHGDRILDLLQSRE 1050

Query: 163  DSGR-----------QLFAGTDTKPAINFPPSVTAQWEEQIKRLYLLLTVKESATEVPIN 222
             SG            QLF        I+FP   +A   EQI+R  LLLTVK+SA ++P N
Sbjct: 1051 GSGEDTGIFMRVIEPQLFESYGEWRCIHFPLPDSASLSEQIQRFLLLLTVKDSAMDIPEN 1110

Query: 223  LEARRRIAFFTNSLFMDMPRAPRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIY 282
            L+ARRR++FF  SLFMDMP AP+VR M+SFSV+TP+Y E+  YS  +L    +  VSII+
Sbjct: 1111 LDARRRLSFFATSLFMDMPDAPKVRNMMSFSVLTPHYQEDINYSTNELH-STKSSVSIIF 1170

Query: 283  YLQKIFPGE-----------------------------------------GMMYYRRALK 292
            Y+QKIFP E                                         GMMY R ALK
Sbjct: 1171 YMQKIFPDEWKNFLERMGCDNLDALKKEGKEEELRNWASFRGQTLSRTVRGMMYCREALK 1230

[Close]

BLAST of Lsi10G007270 vs. ExPASy TrEMBL
Match: A0A0A0LM93 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G302250 PE=4 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 8.4e-131
Identity = 260/336 (77.38%), Postives = 268/336 (79.76%), Query Frame = 0


Query: 1   MKQGDGSLASSLFIRSQLKNNPMASLFTSQQGRYGPLCFLLYEYMKCAVIECYESFKNVL 60
           MKQG GSLASSLF+RSQ KNNPMASLFTS+Q                 VIECYESFKNVL
Sbjct: 1   MKQGGGSLASSLFLRSQSKNNPMASLFTSEQ-----------------VIECYESFKNVL 60

Query: 61  NVLVVGENEKRIIGTIIKEVENNIAKNTLLTNFKMGPLLSLCQKFVELVEILKDGDPSKQ 120
           NVLVVGENEKRIIGTIIKEVENNI KNTLLTNFKMGPLL LC+KFVELVEILKDGDPSK+
Sbjct: 61  NVLVVGENEKRIIGTIIKEVENNIGKNTLLTNFKMGPLLILCKKFVELVEILKDGDPSKR 120

Query: 121 DTVVLLLQDMLEVVTRDMMHNEVRELAELGHNKDSGRQLFAGTDTKPAINFPPSVTAQWE 180
           D VVLLLQDMLEVVTRDMM NEVRELAELGHNKDSGRQLFAGTDT+PAINFPPSVTAQWE
Sbjct: 121 DIVVLLLQDMLEVVTRDMMLNEVRELAELGHNKDSGRQLFAGTDTRPAINFPPSVTAQWE 180

Query: 181 EQIKRLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPRAPRVRKMLSFSVMTPYYS 240
           EQI+RLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPRAPRVRKMLSFSVMTPYY 
Sbjct: 181 EQIRRLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPRAPRVRKMLSFSVMTPYYG 240

Query: 241 EETVYSKTDLEMENEDGVSIIYYLQKIFPGE----------------------------- 295
           EETVYSKTDLEMENEDGVSIIYYLQKI+P E                             
Sbjct: 241 EETVYSKTDLEMENEDGVSIIYYLQKIYPDEWNNFMERLNCKKDSEIWENEENILHLRHW 300

[Close]

BLAST of Lsi10G007270 vs. ExPASy TrEMBL
Match: A0A1S4E305 (1,3-beta-glucan synthase OS=Cucumis melo OX=3656 GN=LOC103499639 PE=3 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.7e-123
Identity = 241/294 (81.97%), Postives = 246/294 (83.67%), Query Frame = 0


Query: 43  EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIAKNTLLTNFKMGPLLSLC 102
           EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNI KNTLLTNFKMGPLL LC
Sbjct: 680 EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIGKNTLLTNFKMGPLLILC 739

Query: 103 QKFVELVEILKDGDPSKQDTVVLLLQDMLEVVTRDMMHNEVRELAELGHNKDSGRQLFAG 162
           QKFVELVEILKDGD SK+DTVVLLLQDMLEVVTRDMM NEVRELAELGHNKDSGRQLFAG
Sbjct: 740 QKFVELVEILKDGDQSKRDTVVLLLQDMLEVVTRDMMLNEVRELAELGHNKDSGRQLFAG 799

Query: 163 TDTKPAINFPPSVTAQWEEQIKRLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR 222
           TDT+PAINFPPSVTAQWEEQI+RLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR
Sbjct: 800 TDTRPAINFPPSVTAQWEEQIRRLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR 859

Query: 223 APRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPGE----------- 282
           APRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKI+P E           
Sbjct: 860 APRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIYPDEWNNFMERLNCK 919

Query: 283 -------------------------------GMMYYRRALKLQAFLDMASESAL 295
                                          GMMYYRRALKLQAFLDMASES +
Sbjct: 920 KDSEILENEENILHLRHWASLRGQTLSRTVRGMMYYRRALKLQAFLDMASESEI 973

[Close]

BLAST of Lsi10G007270 vs. ExPASy TrEMBL
Match: A0A6J1DB98 (1,3-beta-glucan synthase OS=Momordica charantia OX=3673 GN=LOC111018891 PE=3 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 4.9e-123
Identity = 239/294 (81.29%), Postives = 247/294 (84.01%), Query Frame = 0


Query: 43   EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIAKNTLLTNFKMGPLLSLC 102
            EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNI+KNTLLTNFKMGPLL LC
Sbjct: 880  EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNISKNTLLTNFKMGPLLILC 939

Query: 103  QKFVELVEILKDGDPSKQDTVVLLLQDMLEVVTRDMMHNEVRELAELGHNKDSGRQLFAG 162
            QKFVELVEILKDGD SKQDTVVL+LQDMLEVVTRDMMHNEVRELAELGHNKDSGRQLFAG
Sbjct: 940  QKFVELVEILKDGDLSKQDTVVLVLQDMLEVVTRDMMHNEVRELAELGHNKDSGRQLFAG 999

Query: 163  TDTKPAINFPPSVTAQWEEQIKRLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR 222
            TDTKPAI+FPP VTAQWEEQI+RLYLLLTVKESAT+VPINLEARRRIAFFTNSLFM+MPR
Sbjct: 1000 TDTKPAISFPPPVTAQWEEQIRRLYLLLTVKESATDVPINLEARRRIAFFTNSLFMEMPR 1059

Query: 223  APRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPGE----------- 282
            APRVRKMLSFSVMTPYYSEETVYSK+DLEMENEDGVSIIYYLQKIFP E           
Sbjct: 1060 APRVRKMLSFSVMTPYYSEETVYSKSDLEMENEDGVSIIYYLQKIFPDEWNNFMERLNCK 1119

Query: 283  -------------------------------GMMYYRRALKLQAFLDMASESAL 295
                                           GMMYYRRALKLQAFLDMASES +
Sbjct: 1120 KDSEIWENEENILQLRHWVSLRGQTLSRTVRGMMYYRRALKLQAFLDMASESEI 1173

[Close]

BLAST of Lsi10G007270 vs. ExPASy TrEMBL
Match: A0A6J1L2I2 (1,3-beta-glucan synthase OS=Cucurbita maxima OX=3661 GN=LOC111499808 PE=3 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 9.3e-122
Identity = 238/294 (80.95%), Postives = 244/294 (82.99%), Query Frame = 0


Query: 43   EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIAKNTLLTNFKMGPLLSLC 102
            EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNI+KNTLLTNFKMG LL LC
Sbjct: 880  EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNISKNTLLTNFKMGALLILC 939

Query: 103  QKFVELVEILKDGDPSKQDTVVLLLQDMLEVVTRDMMHNEVRELAELGHNKDSGRQLFAG 162
            QKFVELVEILKDGD SKQDTVV LLQDMLEVVTRDMM NEVRE+ ELGHNKDSGRQLFAG
Sbjct: 940  QKFVELVEILKDGDLSKQDTVVFLLQDMLEVVTRDMMLNEVREMVELGHNKDSGRQLFAG 999

Query: 163  TDTKPAINFPPSVTAQWEEQIKRLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR 222
            TDT+PAINFPP+VTAQWEEQI+RLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR
Sbjct: 1000 TDTRPAINFPPTVTAQWEEQIRRLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR 1059

Query: 223  APRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPGE----------- 282
            APRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFP E           
Sbjct: 1060 APRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPDEWNNFIERLNCK 1119

Query: 283  -------------------------------GMMYYRRALKLQAFLDMASESAL 295
                                           GMMYYRRALKLQAFLDMASES +
Sbjct: 1120 KDSEIWENEENILHLRHWASLRGQTLSRTVRGMMYYRRALKLQAFLDMASESEI 1173

[Close]

BLAST of Lsi10G007270 vs. ExPASy TrEMBL
Match: A0A6J1G7S2 (1,3-beta-glucan synthase OS=Cucurbita moschata OX=3662 GN=LOC111451616 PE=3 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 9.3e-122
Identity = 238/294 (80.95%), Postives = 244/294 (82.99%), Query Frame = 0


Query: 43   EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIAKNTLLTNFKMGPLLSLC 102
            EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNI+KNTLLTNFKMG LL LC
Sbjct: 880  EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNISKNTLLTNFKMGALLILC 939

Query: 103  QKFVELVEILKDGDPSKQDTVVLLLQDMLEVVTRDMMHNEVRELAELGHNKDSGRQLFAG 162
            QKFVELVEILKDGD SKQDTVV LLQDMLEVVTRDMM NEVRE+ ELGHNKDSGRQLFAG
Sbjct: 940  QKFVELVEILKDGDLSKQDTVVFLLQDMLEVVTRDMMLNEVREMVELGHNKDSGRQLFAG 999

Query: 163  TDTKPAINFPPSVTAQWEEQIKRLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR 222
            TDT+PAINFPP+VTAQWEEQI+RLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR
Sbjct: 1000 TDTRPAINFPPTVTAQWEEQIRRLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR 1059

Query: 223  APRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPGE----------- 282
            APRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFP E           
Sbjct: 1060 APRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPDEWNNFIERLNCK 1119

Query: 283  -------------------------------GMMYYRRALKLQAFLDMASESAL 295
                                           GMMYYRRALKLQAFLDMASES +
Sbjct: 1120 KDSEIWENEENILHLRHWASLRGQTLSRTVRGMMYYRRALKLQAFLDMASESEI 1173

[Close]

BLAST of Lsi10G007270 vs. NCBI nr
Match: XP_038901615.1 (LOW QUALITY PROTEIN: callose synthase 5 [Benincasa hispida])

HSP 1 Score: 453.8 bits (1166), Expect = 1.2e-123
Identity = 241/294 (81.97%), Postives = 247/294 (84.01%), Query Frame = 0


Query: 43   EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIAKNTLLTNFKMGPLLSLC 102
            EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNI KNTLLTNF+MGPLL LC
Sbjct: 880  EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIGKNTLLTNFRMGPLLILC 939

Query: 103  QKFVELVEILKDGDPSKQDTVVLLLQDMLEVVTRDMMHNEVRELAELGHNKDSGRQLFAG 162
            +KFVELVEILKDGDPSKQD+VVLLLQDMLEVVTRDMM NEVRELAELGHNKDSGRQLFAG
Sbjct: 940  KKFVELVEILKDGDPSKQDSVVLLLQDMLEVVTRDMMLNEVRELAELGHNKDSGRQLFAG 999

Query: 163  TDTKPAINFPPSVTAQWEEQIKRLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR 222
            TDT+PAINFPPSVTAQWEEQI+RLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR
Sbjct: 1000 TDTRPAINFPPSVTAQWEEQIRRLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR 1059

Query: 223  APRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPGE----------- 282
            APRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFP E           
Sbjct: 1060 APRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPDEWNNFMERLNCK 1119

Query: 283  -------------------------------GMMYYRRALKLQAFLDMASESAL 295
                                           GMMYYRRALKLQAFLDMASES +
Sbjct: 1120 KDSEIWENEENILHLRHWASLRGQTLSRTVRGMMYYRRALKLQAFLDMASESEI 1173

[Close]

BLAST of Lsi10G007270 vs. NCBI nr
Match: XP_016902607.1 (PREDICTED: callose synthase 5-like [Cucumis melo])

HSP 1 Score: 452.2 bits (1162), Expect = 3.5e-123
Identity = 241/294 (81.97%), Postives = 246/294 (83.67%), Query Frame = 0


Query: 43  EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIAKNTLLTNFKMGPLLSLC 102
           EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNI KNTLLTNFKMGPLL LC
Sbjct: 680 EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIGKNTLLTNFKMGPLLILC 739

Query: 103 QKFVELVEILKDGDPSKQDTVVLLLQDMLEVVTRDMMHNEVRELAELGHNKDSGRQLFAG 162
           QKFVELVEILKDGD SK+DTVVLLLQDMLEVVTRDMM NEVRELAELGHNKDSGRQLFAG
Sbjct: 740 QKFVELVEILKDGDQSKRDTVVLLLQDMLEVVTRDMMLNEVRELAELGHNKDSGRQLFAG 799

Query: 163 TDTKPAINFPPSVTAQWEEQIKRLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR 222
           TDT+PAINFPPSVTAQWEEQI+RLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR
Sbjct: 800 TDTRPAINFPPSVTAQWEEQIRRLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR 859

Query: 223 APRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPGE----------- 282
           APRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKI+P E           
Sbjct: 860 APRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIYPDEWNNFMERLNCK 919

Query: 283 -------------------------------GMMYYRRALKLQAFLDMASESAL 295
                                          GMMYYRRALKLQAFLDMASES +
Sbjct: 920 KDSEILENEENILHLRHWASLRGQTLSRTVRGMMYYRRALKLQAFLDMASESEI 973

[Close]

BLAST of Lsi10G007270 vs. NCBI nr
Match: KGN62227.2 (hypothetical protein Csa_006766 [Cucumis sativus])

HSP 1 Score: 451.1 bits (1159), Expect = 7.8e-123
Identity = 240/294 (81.63%), Postives = 245/294 (83.33%), Query Frame = 0


Query: 43   EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIAKNTLLTNFKMGPLLSLC 102
            EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNI KNTLLTNFKMGPLL LC
Sbjct: 855  EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIGKNTLLTNFKMGPLLILC 914

Query: 103  QKFVELVEILKDGDPSKQDTVVLLLQDMLEVVTRDMMHNEVRELAELGHNKDSGRQLFAG 162
            +KFVELVEILKDGDPSK+D VVLLLQDMLEVVTRDMM NEVRELAELGHNKDSGRQLFAG
Sbjct: 915  KKFVELVEILKDGDPSKRDIVVLLLQDMLEVVTRDMMLNEVRELAELGHNKDSGRQLFAG 974

Query: 163  TDTKPAINFPPSVTAQWEEQIKRLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR 222
            TDTKPAINFPPSVTAQWEEQI+RLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR
Sbjct: 975  TDTKPAINFPPSVTAQWEEQIRRLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR 1034

Query: 223  APRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPGE----------- 282
            APRVRKMLSFSVMTPYY EETVYSKTDLEMENEDGVSIIYYLQKI+P E           
Sbjct: 1035 APRVRKMLSFSVMTPYYGEETVYSKTDLEMENEDGVSIIYYLQKIYPDEWNNFMERLNCK 1094

Query: 283  -------------------------------GMMYYRRALKLQAFLDMASESAL 295
                                           GMMYYRRALKLQAFLDMASES +
Sbjct: 1095 KDSEIWENEENILHLRHWASLRGQTLSRTVRGMMYYRRALKLQAFLDMASESEI 1148

[Close]

BLAST of Lsi10G007270 vs. NCBI nr
Match: XP_031736648.1 (callose synthase 5-like [Cucumis sativus])

HSP 1 Score: 451.1 bits (1159), Expect = 7.8e-123
Identity = 240/294 (81.63%), Postives = 245/294 (83.33%), Query Frame = 0


Query: 43   EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIAKNTLLTNFKMGPLLSLC 102
            EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNI KNTLLTNFKMGPLL LC
Sbjct: 880  EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIGKNTLLTNFKMGPLLILC 939

Query: 103  QKFVELVEILKDGDPSKQDTVVLLLQDMLEVVTRDMMHNEVRELAELGHNKDSGRQLFAG 162
            +KFVELVEILKDGDPSK+D VVLLLQDMLEVVTRDMM NEVRELAELGHNKDSGRQLFAG
Sbjct: 940  KKFVELVEILKDGDPSKRDIVVLLLQDMLEVVTRDMMLNEVRELAELGHNKDSGRQLFAG 999

Query: 163  TDTKPAINFPPSVTAQWEEQIKRLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR 222
            TDTKPAINFPPSVTAQWEEQI+RLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR
Sbjct: 1000 TDTKPAINFPPSVTAQWEEQIRRLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR 1059

Query: 223  APRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPGE----------- 282
            APRVRKMLSFSVMTPYY EETVYSKTDLEMENEDGVSIIYYLQKI+P E           
Sbjct: 1060 APRVRKMLSFSVMTPYYGEETVYSKTDLEMENEDGVSIIYYLQKIYPDEWNNFMERLNCK 1119

Query: 283  -------------------------------GMMYYRRALKLQAFLDMASESAL 295
                                           GMMYYRRALKLQAFLDMASES +
Sbjct: 1120 KDSEIWENEENILHLRHWASLRGQTLSRTVRGMMYYRRALKLQAFLDMASESEI 1173

[Close]

BLAST of Lsi10G007270 vs. NCBI nr
Match: XP_022150844.1 (callose synthase 5 [Momordica charantia])

HSP 1 Score: 450.7 bits (1158), Expect = 1.0e-122
Identity = 239/294 (81.29%), Postives = 247/294 (84.01%), Query Frame = 0


Query: 43   EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIAKNTLLTNFKMGPLLSLC 102
            EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNI+KNTLLTNFKMGPLL LC
Sbjct: 880  EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNISKNTLLTNFKMGPLLILC 939

Query: 103  QKFVELVEILKDGDPSKQDTVVLLLQDMLEVVTRDMMHNEVRELAELGHNKDSGRQLFAG 162
            QKFVELVEILKDGD SKQDTVVL+LQDMLEVVTRDMMHNEVRELAELGHNKDSGRQLFAG
Sbjct: 940  QKFVELVEILKDGDLSKQDTVVLVLQDMLEVVTRDMMHNEVRELAELGHNKDSGRQLFAG 999

Query: 163  TDTKPAINFPPSVTAQWEEQIKRLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMPR 222
            TDTKPAI+FPP VTAQWEEQI+RLYLLLTVKESAT+VPINLEARRRIAFFTNSLFM+MPR
Sbjct: 1000 TDTKPAISFPPPVTAQWEEQIRRLYLLLTVKESATDVPINLEARRRIAFFTNSLFMEMPR 1059

Query: 223  APRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPGE----------- 282
            APRVRKMLSFSVMTPYYSEETVYSK+DLEMENEDGVSIIYYLQKIFP E           
Sbjct: 1060 APRVRKMLSFSVMTPYYSEETVYSKSDLEMENEDGVSIIYYLQKIFPDEWNNFMERLNCK 1119

Query: 283  -------------------------------GMMYYRRALKLQAFLDMASESAL 295
                                           GMMYYRRALKLQAFLDMASES +
Sbjct: 1120 KDSEIWENEENILQLRHWVSLRGQTLSRTVRGMMYYRRALKLQAFLDMASESEI 1173

[Close]

BLAST of Lsi10G007270 vs. TAIR 10
Match: AT2G13680.1 (callose synthase 5 )

HSP 1 Score: 390.2 bits (1001), Expect = 1.5e-108
Identity = 207/295 (70.17%), Postives = 229/295 (77.63%), Query Frame = 0


Query: 43   EYMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIAKNTLLTNFKMGPLLSLC 102
            EYMKCAVIECYESFK+VL+ LV+GENEKRIIG IIKEVE+NI+KN+ L+NF+M PL +LC
Sbjct: 883  EYMKCAVIECYESFKHVLHTLVIGENEKRIIGIIIKEVESNISKNSFLSNFRMAPLPALC 942

Query: 103  QKFVELVEILKDGDPSKQDTVVLLLQDMLEVVTRDMMHNEVRELAELGH-NKDSGRQLFA 162
             KFVELV ILK+ DP+K+DTVVLLLQDMLEVVTRDMM NE REL ELGH NK+SGRQLFA
Sbjct: 943  SKFVELVGILKNADPAKRDTVVLLLQDMLEVVTRDMMQNENRELVELGHTNKESGRQLFA 1002

Query: 163  GTDTKPAINFPPSVTAQWEEQIKRLYLLLTVKESATEVPINLEARRRIAFFTNSLFMDMP 222
            GTD KPAI FPP  TAQW EQI RL+LLLTVKESA +VP NLEA+RRIAFFTNSLFMDMP
Sbjct: 1003 GTDAKPAILFPPVATAQWHEQISRLHLLLTVKESAMDVPTNLEAQRRIAFFTNSLFMDMP 1062

Query: 223  RAPRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPGE---------- 282
            RAPRVR MLSFSV+TPYYSEETVYSK DLEMENEDGVS++YYLQKIFP E          
Sbjct: 1063 RAPRVRNMLSFSVLTPYYSEETVYSKNDLEMENEDGVSVVYYLQKIFPDEWTNFLERLDC 1122

Query: 283  --------------------------------GMMYYRRALKLQAFLDMASESAL 295
                                            GMMYYRRALKLQAFLDMA+E+ +
Sbjct: 1123 KDETSVLESEENILQLRHWVSLRGQTLFRTVRGMMYYRRALKLQAFLDMANETEI 1177

[Close]

BLAST of Lsi10G007270 vs. TAIR 10
Match: AT5G13000.2 (glucan synthase-like 12 )

HSP 1 Score: 245.0 bits (624), Expect = 7.9e-65
Identity = 146/303 (48.18%), Postives = 180/303 (59.41%), Query Frame = 0


Query: 44   YMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIAKNTLLTNFKMGPLLSLCQ 103
            YMKCAV ECY SFKN++  +V G  EK +I  I  EV+ +I    L+  +KM  L SL  
Sbjct: 893  YMKCAVRECYASFKNIIKFVVQGNREKEVIEIIFAEVDKHIDTGDLIQEYKMSALPSLYD 952

Query: 104  KFVELVEILKDGDPSKQDTVVLLLQDMLEVVTRDMMHNE--VRELAELGHN--------- 163
             FV+L++ L D     +D VV+L QDMLEVVTRD+M  +  +  L +  H          
Sbjct: 953  HFVKLIKYLLDNKEEDRDHVVILFQDMLEVVTRDIMMEDYNISSLVDSSHGGTWHGGMIP 1012

Query: 164  KDSGRQLFAGTDTKPAINFP-PSVTAQWEEQIKRLYLLLTVKESATEVPINLEARRRIAF 223
             +   QLFA +    AI FP   VT  W+E+IKR+YLLLT KESA +VP NLEARRRI+F
Sbjct: 1013 LEQQYQLFASSG---AIRFPIEPVTEAWKEKIKRIYLLLTTKESAMDVPSNLEARRRISF 1072

Query: 224  FTNSLFMDMPRAPRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPGE 283
            F+NSLFMDMP AP+VR MLSFSV+TPYY+EE ++S  DLE  NEDGVSI++YLQKIFP E
Sbjct: 1073 FSNSLFMDMPMAPKVRNMLSFSVLTPYYTEEVLFSLRDLETPNEDGVSILFYLQKIFPDE 1132

Query: 284  ----------------------------------------GMMYYRRALKLQAFLDMASE 295
                                                    GMMYYR+AL+LQAFLDMA  
Sbjct: 1133 WNNFLERVKCLSEEELKESDELEEELRLWASYRGQTLTRTGMMYYRKALELQAFLDMAMH 1192

[Close]

BLAST of Lsi10G007270 vs. TAIR 10
Match: AT5G13000.1 (glucan synthase-like 12 )

HSP 1 Score: 244.2 bits (622), Expect = 1.3e-64
Identity = 146/305 (47.87%), Postives = 180/305 (59.02%), Query Frame = 0


Query: 44   YMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIAKNTLLTNFKMGPLLSLCQ 103
            YMKCAV ECY SFKN++  +V G  EK +I  I  EV+ +I    L+  +KM  L SL  
Sbjct: 893  YMKCAVRECYASFKNIIKFVVQGNREKEVIEIIFAEVDKHIDTGDLIQEYKMSALPSLYD 952

Query: 104  KFVELVEILKDGDPSKQDTVVLLLQDMLEVVTRDMMHNE--VRELAELGHN--------- 163
             FV+L++ L D     +D VV+L QDMLEVVTRD+M  +  +  L +  H          
Sbjct: 953  HFVKLIKYLLDNKEEDRDHVVILFQDMLEVVTRDIMMEDYNISSLVDSSHGGTWHGGMIP 1012

Query: 164  KDSGRQLFAGTDTKPAINFP-PSVTAQWEEQIKRLYLLLTVKESATEVPINLEARRRIAF 223
             +   QLFA +    AI FP   VT  W+E+IKR+YLLLT KESA +VP NLEARRRI+F
Sbjct: 1013 LEQQYQLFASSG---AIRFPIEPVTEAWKEKIKRIYLLLTTKESAMDVPSNLEARRRISF 1072

Query: 224  FTNSLFMDMPRAPRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPGE 283
            F+NSLFMDMP AP+VR MLSFSV+TPYY+EE ++S  DLE  NEDGVSI++YLQKIFP E
Sbjct: 1073 FSNSLFMDMPMAPKVRNMLSFSVLTPYYTEEVLFSLRDLETPNEDGVSILFYLQKIFPDE 1132

Query: 284  ------------------------------------------GMMYYRRALKLQAFLDMA 295
                                                      GMMYYR+AL+LQAFLDMA
Sbjct: 1133 WNNFLERVKCLSEEELKESDELEEELRLWASYRGQTLTRTVRGMMYYRKALELQAFLDMA 1192

[Close]

BLAST of Lsi10G007270 vs. TAIR 10
Match: AT1G05570.1 (callose synthase 1 )

HSP 1 Score: 241.5 bits (615), Expect = 8.7e-64
Identity = 139/299 (46.49%), Postives = 179/299 (59.87%), Query Frame = 0


Query: 44   YMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIAKNTLLTNFKMGPLLSLCQ 103
            YM CAV ECY SFKN++N LVVGE E ++I  I  +++ +I K TL+T   +  L  L  
Sbjct: 887  YMTCAVRECYASFKNLINYLVVGEREGQVINDIFSKIDEHIEKETLITELNLSALPDLYG 946

Query: 104  KFVELVEILKDGDPSKQDTVVLLLQDMLEVVTRDMMHNEVRELAELGHN-----KDSGRQ 163
            +FV L+E L +     +D +V++L +MLE+VTRD+M  EV  L E  HN      D    
Sbjct: 947  QFVRLIEYLLENREEDKDQIVIVLLNMLELVTRDIMEEEVPSLLETAHNGSYVKYDVMTP 1006

Query: 164  LFAGTDTKPAINFPP-SVTAQWEEQIKRLYLLLTVKESATEVPINLEARRRIAFFTNSLF 223
            L         + FP  S T  W+E+IKRL+LLLTVKESA +VP NLEARRR+ FF+NSLF
Sbjct: 1007 LHQQRKYFSQLRFPVYSQTEAWKEKIKRLHLLLTVKESAMDVPSNLEARRRLTFFSNSLF 1066

Query: 224  MDMPRAPRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPGE------ 283
            MDMP AP++R MLSFSV+TPY+SE+ ++S   LE +NEDGVSI++YLQKIFP E      
Sbjct: 1067 MDMPPAPKIRNMLSFSVLTPYFSEDVLFSIFGLEQQNEDGVSILFYLQKIFPDEWTNFLE 1126

Query: 284  ------------------------------------GMMYYRRALKLQAFLDMASESAL 295
                                                GMMYYR+AL+LQAFLDMA +  L
Sbjct: 1127 RVKCGNEEELRAREDLEEELRLWASYRGQTLTKTVRGMMYYRKALELQAFLDMAKDEEL 1185

[Close]

BLAST of Lsi10G007270 vs. TAIR 10
Match: AT1G05570.2 (callose synthase 1 )

HSP 1 Score: 241.5 bits (615), Expect = 8.7e-64
Identity = 139/299 (46.49%), Postives = 179/299 (59.87%), Query Frame = 0


Query: 44   YMKCAVIECYESFKNVLNVLVVGENEKRIIGTIIKEVENNIAKNTLLTNFKMGPLLSLCQ 103
            YM CAV ECY SFKN++N LVVGE E ++I  I  +++ +I K TL+T   +  L  L  
Sbjct: 846  YMTCAVRECYASFKNLINYLVVGEREGQVINDIFSKIDEHIEKETLITELNLSALPDLYG 905

Query: 104  KFVELVEILKDGDPSKQDTVVLLLQDMLEVVTRDMMHNEVRELAELGHN-----KDSGRQ 163
            +FV L+E L +     +D +V++L +MLE+VTRD+M  EV  L E  HN      D    
Sbjct: 906  QFVRLIEYLLENREEDKDQIVIVLLNMLELVTRDIMEEEVPSLLETAHNGSYVKYDVMTP 965

Query: 164  LFAGTDTKPAINFPP-SVTAQWEEQIKRLYLLLTVKESATEVPINLEARRRIAFFTNSLF 223
            L         + FP  S T  W+E+IKRL+LLLTVKESA +VP NLEARRR+ FF+NSLF
Sbjct: 966  LHQQRKYFSQLRFPVYSQTEAWKEKIKRLHLLLTVKESAMDVPSNLEARRRLTFFSNSLF 1025

Query: 224  MDMPRAPRVRKMLSFSVMTPYYSEETVYSKTDLEMENEDGVSIIYYLQKIFPGE------ 283
            MDMP AP++R MLSFSV+TPY+SE+ ++S   LE +NEDGVSI++YLQKIFP E      
Sbjct: 1026 MDMPPAPKIRNMLSFSVLTPYFSEDVLFSIFGLEQQNEDGVSILFYLQKIFPDEWTNFLE 1085

Query: 284  ------------------------------------GMMYYRRALKLQAFLDMASESAL 295
                                                GMMYYR+AL+LQAFLDMA +  L
Sbjct: 1086 RVKCGNEEELRAREDLEEELRLWASYRGQTLTKTVRGMMYYRKALELQAFLDMAKDEEL 1144

The following BLAST results are available for this feature:

BLAST of Lsi10G007270 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3B724	2.1e-107	70.17	Callose synthase 5 OS=Arabidopsis thaliana OX=3702 GN=CALS5 PE=1 SV=1	[more]
Q9LXT9	1.9e-63	47.87	Callose synthase 3 OS=Arabidopsis thaliana OX=3702 GN=CALS3 PE=3 SV=3	[more]
Q9AUE0	1.2e-62	46.49	Callose synthase 1 OS=Arabidopsis thaliana OX=3702 GN=CALS1 PE=1 SV=2	[more]
Q9SL03	1.4e-61	46.15	Callose synthase 2 OS=Arabidopsis thaliana OX=3702 GN=CALS2 PE=2 SV=3	[more]
Q9LUD7	1.1e-50	42.26	Putative callose synthase 8 OS=Arabidopsis thaliana OX=3702 GN=CALS8 PE=3 SV=2	[more]

BLAST of Lsi10G007270 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LM93	8.4e-131	77.38	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G302250 PE=4 SV=1	[more]
A0A1S4E305	1.7e-123	81.97	1,3-beta-glucan synthase OS=Cucumis melo OX=3656 GN=LOC103499639 PE=3 SV=1	[more]
A0A6J1DB98	4.9e-123	81.29	1,3-beta-glucan synthase OS=Momordica charantia OX=3673 GN=LOC111018891 PE=3 SV=...	[more]
A0A6J1L2I2	9.3e-122	80.95	1,3-beta-glucan synthase OS=Cucurbita maxima OX=3661 GN=LOC111499808 PE=3 SV=1	[more]
A0A6J1G7S2	9.3e-122	80.95	1,3-beta-glucan synthase OS=Cucurbita moschata OX=3662 GN=LOC111451616 PE=3 SV=1	[more]

BLAST of Lsi10G007270 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038901615.1	1.2e-123	81.97	LOW QUALITY PROTEIN: callose synthase 5 [Benincasa hispida]	[more]
XP_016902607.1	3.5e-123	81.97	PREDICTED: callose synthase 5-like [Cucumis melo]	[more]
KGN62227.2	7.8e-123	81.63	hypothetical protein Csa_006766 [Cucumis sativus]	[more]
XP_031736648.1	7.8e-123	81.63	callose synthase 5-like [Cucumis sativus]	[more]
XP_022150844.1	1.0e-122	81.29	callose synthase 5 [Momordica charantia]	[more]

BLAST of Lsi10G007270 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G13680.1	1.5e-108	70.17	callose synthase 5	[more]
AT5G13000.2	7.9e-65	48.18	glucan synthase-like 12	[more]
AT5G13000.1	1.3e-64	47.87	glucan synthase-like 12	[more]
AT1G05570.1	8.7e-64	46.49	callose synthase 1	[more]
AT1G05570.2	8.7e-64	46.49	callose synthase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003440	Glycosyl transferase, family 48	PFAM	PF02364	Glucan_synthase	coord: 199..271 e-value: 1.3E-21 score: 76.6
None	No IPR available	PANTHER	PTHR12741	LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1	coord: 42..271
None	No IPR available	PANTHER	PTHR12741:SF53	GLUCAN SYNTHASE-LIKE PROTEIN	coord: 42..271
None	No IPR available	PANTHER	PTHR12741	LYST-INTERACTING PROTEIN LIP5 DOPAMINE RESPONSIVE PROTEIN DRG-1	coord: 272..293
None	No IPR available	PANTHER	PTHR12741:SF53	GLUCAN SYNTHASE-LIKE PROTEIN	coord: 272..293

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G007270.1	Lsi10G007270.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006075	(1->3)-beta-D-glucan biosynthetic process
biological_process	GO:0071555	cell wall organization
biological_process	GO:0051607	defense response to virus
biological_process	GO:0031047	gene silencing by RNA
biological_process	GO:0008360	regulation of cell shape
cellular_component	GO:0000148	1,3-beta-D-glucan synthase complex
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0003843	1,3-beta-D-glucan synthase activity