Lsi10G007180 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi10G007180
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Pectinesterase
Location	chr10: 10056507 .. 10059556 (-)
RNA-Seq Expression	Lsi10G007180
Synteny	Lsi10G007180

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATAGAAAAGTCCAACGCAACAGAAAAGTTTCTTTCCTTATTTTCTCGAAGACATTGAAGGACAACAAAAAAAACGCACAAAAAAACTCCAATTCCTCCGCCGTAGACTGCAGCGGTCACCACTTTAACCGCCTCCACAACCACCCGTGACTTCTGTTTTCCTCTCCCCGTGACTCCCTTTCTCCATTTTTGGCCATTATCACCTTTAATCTCTCTCTCTTCACATTCCCCATTATAAATATTCCATTTCCATTTCCCACCAATGCTCCCCAGAAAACCCCCTTTCCCCATTTCCAAATCCACTCTGCTTTTCCTTTCCATGGCTTCCCTCTCCTTCCTCTCTTTCCTCTCCCTCTTCTTCTTCTTCTTCCTCTCTTTCTCCTCCTCCGCCGCCCGCCACCGCCACCACCTCTCAACCCATTTCACCAACCCACCTTCCAACGACGACATCCTTCACGCCTGCAAGGCCACCCGTTTTCCCGATCAATGCGGATCTTCCCTTTCTCGCTCCTCTCTCCCTCCCAATCCCACCCCTCTTCAGATCCTCCAATCCGCCGTCGCCCTCTCATCCGACGGCTTAGCCACTGCTCAGTCCATGGTTAATCAGATTCTCGATTCCTCTGCTAAGAATCTCAATCGGACCACCGCTGCCAAGAATTGCCTTGAGGTGCTGCGGAATTCCGATTACCGAATTTCTCTCACCAACGACGCTCTCCGCCGTGGTCGGACCAAGGATGCCCGCGCCTGGCTGAGCGCGTCCCTGCTCTACCATTACGATTGCTGGTCTGCGCTCAAGTACGCTAACGACACTCAATTGGTTGACAAAACCATGGCGTTTCTTGATTCTCTAATCGGCCTCTCCAGCAACGCGCTAAGTATGTTGGTGGCTTACGACATTTCCGGCAACGATACGGCGGCGTGGACACCACCGAGGACGGAGCGGGATGGGTTCTGGGAGGGCGGTGGTGGTGGATCTGGGTTGGGGTTTAGCGGTGGGTTTCCGTCGAAGGTGGCGGCTGATGCGACGGTGTGCAAGGATGGCGCTGGGGGATGTTACGGGACTGTTCAGGAAGCTGTGGACGCGGCGCCAGAGAATGCGGCGGCGCGTAGGTTTGTGATTCATATTAAAGAAGGTGTTTATGAGGAAATCGTGAGGGTTCCGTTAGAGAAGAAGAATGTGGTGTTTTTAGGAGATGGGATGGGCAAAACGGTAATTACATGCTCACTTAATGTGGGCCAGCCTGGGATTTCCACTTACAATACCGCCACCGTTGGTGAGTTTTATTTATTTATTATTTTCTTAATTACAAGTTTTTTTTTTCTTCTTCTTCTTCTGAGATGCTTTTAATTACAAGTTTAGTCTTTAATTTTTTCAAGAAAAAAAAGTTAGTCTTTTAATTCTGAAATTTTTGTATATTTAATTTTAAAAATGGTAAAAGTATTTCATTTCTAGCAAATTAATTTTTTTTGCTCTAATACATATGAAATTACACTTTGTCTAATAGAACTTTAAATTTTCAATTTTATATCTAAATTCATGCATTTTTAAGGACTTTTAGATTTGAGTCACGGTGATGAACTATTTAAGATTTAAGATAGTAAATTATTTAAGATTTAATATCTTATGATTTTGTTTTTCTTTTTTTTGACAATTCTATTAGGTGCGTACACTCAAAGGATGTCTAAAAAACATTATTGTTAGTCTTGAAGAGTGTATCTATTCTCAAAATTTAGAACTTATTGTTAAAACTAAGTAGTTAAAATTGGTTGATATATATAGATGACCTGAATTCTTTGTGGCTGTGTATTGATCAAAAGTGGCATGGATGACTTGAATTCTTTATATTCGATCTGATCGAAATCGAAGTAGTATATTGTTGGATATTTAAAGATTGAAAAATGTTGCCAATTTAGTATAAAGTTGATGTTGTTTTGTGCAGGAGTACTTGGTGATGGATTCATGGCTAGCGGTCTCACCATTCAGAACACAGCCGGGCCTGATGCCCATCAAGCTGTGGCCTTTAGATCAGACAGTGATCTCTCAGTCATTCAAGACTGTGAGTTTATAGGCAACCAAGACACTCTCTACGCTCATTCCCTTCGCCAATTCTACAAATCTTGCAAGATTCAAGGCAATGTAGATTTCATCTTTGGAAACTCAGCTTCCATCTTCCAAGATTGCGAGATCCTAGTCCGCCCTCGACAGCTCAAGCCCGAGAAAGGCGAAAACAACGCCATCACTGCTCAGGGCAGGACCGACCCTGCTCAATCGACTGGTTTCGTGTTTCAAAACTGCCTCATAAATGGCACCCAGGAGTACATGTCATTGTACTACAGCAAGCCTAAAGTGCATAAGAACTTCTTGGGGAGGCCATGGAAGGAGTACTCGAGGACGGTTTTCATTCATTGTAATCTCGAGGCTCTTGTCACGCCGTCGGGGTGGATGCCGTGGAGCGGGGATTTTGCTTTGAAGACTCTCTTCTATGGAGAGTTTGAGAATTCTGGAGCGGGGTCAAAGACGTCGGAGAGAGTCGGGTGGAGTAGTCAAATTCCAGCACAACATGTTTTTAGTTACTCTGTTCAGAATTTCATTCAGGGAGATGAATGGATTCCTTCATCTGAGTCATCTAATGAACAAAAAATGTTGAACAAGAAAAGTAAAGACGTGAAATTAGATTAGATTTGGAAATCCCATTGTAATTACAAACCTCCCTATGGAACTACCAATTATTAGTCTTACATAAAATTACCAAATAATCCACCACTCATCTCTGTTGTATCAATAATTTTGATTGAAATTTGCATCCGTTTTATTGGGTCAGTGTATGGTTGAAAAGGAGTGGGTTAGTTTTATTGGGTGAGTGTTCAGTTGAAGTGTTTGAATTATATGAAGTGGAGGGGTCCAAAATGGTACATAATTGTCAATGTTTTTAGGTTTTTTGGGTAACATATTCAATGGGTTACTAAAATATTGGTAAAATATTTACTCCCATTAAGAAGGTGCTTGATTCCACTGAGGTTGTATTTATTTCATTATTTTTTAATTTTTAG

mRNA sequence

ATAGAAAAGTCCAACGCAACAGAAAAGTTTCTTTCCTTATTTTCTCGAAGACATTGAAGGACAACAAAAAAAACGCACAAAAAAACTCCAATTCCTCCGCCGTAGACTGCAGCGGTCACCACTTTAACCGCCTCCACAACCACCCGTGACTTCTGTTTTCCTCTCCCCGTGACTCCCTTTCTCCATTTTTGGCCATTATCACCTTTAATCTCTCTCTCTTCACATTCCCCATTATAAATATTCCATTTCCATTTCCCACCAATGCTCCCCAGAAAACCCCCTTTCCCCATTTCCAAATCCACTCTGCTTTTCCTTTCCATGGCTTCCCTCTCCTTCCTCTCTTTCCTCTCCCTCTTCTTCTTCTTCTTCCTCTCTTTCTCCTCCTCCGCCGCCCGCCACCGCCACCACCTCTCAACCCATTTCACCAACCCACCTTCCAACGACGACATCCTTCACGCCTGCAAGGCCACCCGTTTTCCCGATCAATGCGGATCTTCCCTTTCTCGCTCCTCTCTCCCTCCCAATCCCACCCCTCTTCAGATCCTCCAATCCGCCGTCGCCCTCTCATCCGACGGCTTAGCCACTGCTCAGTCCATGGTTAATCAGATTCTCGATTCCTCTGCTAAGAATCTCAATCGGACCACCGCTGCCAAGAATTGCCTTGAGGTGCTGCGGAATTCCGATTACCGAATTTCTCTCACCAACGACGCTCTCCGCCGTGGTCGGACCAAGGATGCCCGCGCCTGGCTGAGCGCGTCCCTGCTCTACCATTACGATTGCTGGTCTGCGCTCAAGTACGCTAACGACACTCAATTGGTTGACAAAACCATGGCGTTTCTTGATTCTCTAATCGGCCTCTCCAGCAACGCGCTAAGTATGTTGGTGGCTTACGACATTTCCGGCAACGATACGGCGGCGTGGACACCACCGAGGACGGAGCGGGATGGGTTCTGGGAGGGCGGTGGTGGTGGATCTGGGTTGGGGTTTAGCGGTGGGTTTCCGTCGAAGGTGGCGGCTGATGCGACGGTGTGCAAGGATGGCGCTGGGGGATGTTACGGGACTGTTCAGGAAGCTGTGGACGCGGCGCCAGAGAATGCGGCGGCGCGTAGGTTTGTGATTCATATTAAAGAAGGTGTTTATGAGGAAATCGTGAGGGTTCCGTTAGAGAAGAAGAATGTGGTGTTTTTAGGAGATGGGATGGGCAAAACGGTAATTACATGCTCACTTAATGTGGGCCAGCCTGGGATTTCCACTTACAATACCGCCACCGTTGGAGTACTTGGTGATGGATTCATGGCTAGCGGTCTCACCATTCAGAACACAGCCGGGCCTGATGCCCATCAAGCTGTGGCCTTTAGATCAGACAGTGATCTCTCAGTCATTCAAGACTGTGAGTTTATAGGCAACCAAGACACTCTCTACGCTCATTCCCTTCGCCAATTCTACAAATCTTGCAAGATTCAAGGCAATGTAGATTTCATCTTTGGAAACTCAGCTTCCATCTTCCAAGATTGCGAGATCCTAGTCCGCCCTCGACAGCTCAAGCCCGAGAAAGGCGAAAACAACGCCATCACTGCTCAGGGCAGGACCGACCCTGCTCAATCGACTGGTTTCGTGTTTCAAAACTGCCTCATAAATGGCACCCAGGAGTACATGTCATTGTACTACAGCAAGCCTAAAGTGCATAAGAACTTCTTGGGGAGGCCATGGAAGGAGTACTCGAGGACGGTTTTCATTCATTGTAATCTCGAGGCTCTTGTCACGCCGTCGGGGTGGATGCCGTGGAGCGGGGATTTTGCTTTGAAGACTCTCTTCTATGGAGAGTTTGAGAATTCTGGAGCGGGGTCAAAGACGTCGGAGAGAGTCGGGTGGAGTAGTCAAATTCCAGCACAACATGTTTTTAGTTACTCTGTTCAGAATTTCATTCAGGGAGATGAATGGATTCCTTCATCTGAGTCATCTAATGAACAAAAAATGTTGAACAAGAAAAGTAAAGACGTGAAATTAGATTAGATTTGGAAATCCCATTGTAATTACAAACCTCCCTATGGAACTACCAATTATTAGTCTTACATAAAATTACCAAATAATCCACCACTCATCTCTGTTGTATCAATAATTTTGATTGAAATTTGCATCCGTTTTATTGGGTCAGTGTATGGTTGAAAAGGAGTGGGTTAGTTTTATTGGGTGAGTGTTCAGTTGAAGTGTTTGAATTATATGAAGTGGAGGGGTCCAAAATGGTACATAATTGTCAATGTTTTTAGGTTTTTTGGGTAACATATTCAATGGGTTACTAAAATATTGGTAAAATATTTACTCCCATTAAGAAGGTGCTTGATTCCACTGAGGTTGTATTTATTTCATTATTTTTTAATTTTTAG

Coding sequence (CDS)

ATGCTCCCCAGAAAACCCCCTTTCCCCATTTCCAAATCCACTCTGCTTTTCCTTTCCATGGCTTCCCTCTCCTTCCTCTCTTTCCTCTCCCTCTTCTTCTTCTTCTTCCTCTCTTTCTCCTCCTCCGCCGCCCGCCACCGCCACCACCTCTCAACCCATTTCACCAACCCACCTTCCAACGACGACATCCTTCACGCCTGCAAGGCCACCCGTTTTCCCGATCAATGCGGATCTTCCCTTTCTCGCTCCTCTCTCCCTCCCAATCCCACCCCTCTTCAGATCCTCCAATCCGCCGTCGCCCTCTCATCCGACGGCTTAGCCACTGCTCAGTCCATGGTTAATCAGATTCTCGATTCCTCTGCTAAGAATCTCAATCGGACCACCGCTGCCAAGAATTGCCTTGAGGTGCTGCGGAATTCCGATTACCGAATTTCTCTCACCAACGACGCTCTCCGCCGTGGTCGGACCAAGGATGCCCGCGCCTGGCTGAGCGCGTCCCTGCTCTACCATTACGATTGCTGGTCTGCGCTCAAGTACGCTAACGACACTCAATTGGTTGACAAAACCATGGCGTTTCTTGATTCTCTAATCGGCCTCTCCAGCAACGCGCTAAGTATGTTGGTGGCTTACGACATTTCCGGCAACGATACGGCGGCGTGGACACCACCGAGGACGGAGCGGGATGGGTTCTGGGAGGGCGGTGGTGGTGGATCTGGGTTGGGGTTTAGCGGTGGGTTTCCGTCGAAGGTGGCGGCTGATGCGACGGTGTGCAAGGATGGCGCTGGGGGATGTTACGGGACTGTTCAGGAAGCTGTGGACGCGGCGCCAGAGAATGCGGCGGCGCGTAGGTTTGTGATTCATATTAAAGAAGGTGTTTATGAGGAAATCGTGAGGGTTCCGTTAGAGAAGAAGAATGTGGTGTTTTTAGGAGATGGGATGGGCAAAACGGTAATTACATGCTCACTTAATGTGGGCCAGCCTGGGATTTCCACTTACAATACCGCCACCGTTGGAGTACTTGGTGATGGATTCATGGCTAGCGGTCTCACCATTCAGAACACAGCCGGGCCTGATGCCCATCAAGCTGTGGCCTTTAGATCAGACAGTGATCTCTCAGTCATTCAAGACTGTGAGTTTATAGGCAACCAAGACACTCTCTACGCTCATTCCCTTCGCCAATTCTACAAATCTTGCAAGATTCAAGGCAATGTAGATTTCATCTTTGGAAACTCAGCTTCCATCTTCCAAGATTGCGAGATCCTAGTCCGCCCTCGACAGCTCAAGCCCGAGAAAGGCGAAAACAACGCCATCACTGCTCAGGGCAGGACCGACCCTGCTCAATCGACTGGTTTCGTGTTTCAAAACTGCCTCATAAATGGCACCCAGGAGTACATGTCATTGTACTACAGCAAGCCTAAAGTGCATAAGAACTTCTTGGGGAGGCCATGGAAGGAGTACTCGAGGACGGTTTTCATTCATTGTAATCTCGAGGCTCTTGTCACGCCGTCGGGGTGGATGCCGTGGAGCGGGGATTTTGCTTTGAAGACTCTCTTCTATGGAGAGTTTGAGAATTCTGGAGCGGGGTCAAAGACGTCGGAGAGAGTCGGGTGGAGTAGTCAAATTCCAGCACAACATGTTTTTAGTTACTCTGTTCAGAATTTCATTCAGGGAGATGAATGGATTCCTTCATCTGAGTCATCTAATGAACAAAAAATGTTGAACAAGAAAAGTAAAGACGTGAAATTAGATTAG

Protein sequence

MLPRKPPFPISKSTLLFLSMASLSFLSFLSLFFFFFLSFSSSAARHRHHLSTHFTNPPSNDDILHACKATRFPDQCGSSLSRSSLPPNPTPLQILQSAVALSSDGLATAQSMVNQILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFWEGGGGGSGLGFSGGFPSKVAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGDGMGKTVITCSLNVGQPGISTYNTATVGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRTVFIHCNLEALVTPSGWMPWSGDFALKTLFYGEFENSGAGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSESSNEQKMLNKKSKDVKLD

Homology

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BLAST of Lsi10G007180 vs. ExPASy Swiss-Prot
Match: Q9LXD9 (Probable pectinesterase/pectinesterase inhibitor 51 OS=Arabidopsis thaliana OX=3702 GN=PME51 PE=2 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 7.3e-205
Identity = 361/544 (66.36%), Postives = 427/544 (78.49%), Query Frame = 0


Query: 31  LFFFFFLSFSSSAARHRHHLSTHFTNP--PSND---DILHACKATRFPDQCGSSLS-RSS 90
           LF  F  S  SS++RH HH ++  T+P  PS+     I  AC ATR+PDQC SSLS +  
Sbjct: 8   LFSLFLFSSPSSSSRHHHHHNSGDTSPVNPSSSLAAQIRLACNATRYPDQCVSSLSEQGR 67

Query: 91  LPPNPTPLQILQSAVALSSDGLATAQSMVNQILDSSAKNLNRTTAAKNCLEVLRNSDYRI 150
           +PP+P P+QI+ SA++ S   L TAQS +  I+DSS  NLNRT AA  CL++L  S++R 
Sbjct: 68  VPPDPKPIQIIHSAISFSFQNLKTAQSKIKSIVDSSVGNLNRTNAANTCLQLLTYSEHRT 127

Query: 151 SLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMAFLDSLIGLSSNAL 210
             T+ AL RG+ KDARAW+SA+L+Y YD WSALKY NDT  V +TM+FLD LI ++SNAL
Sbjct: 128 QSTDQALTRGKIKDARAWMSAALVYQYDSWSALKYVNDTSQVGETMSFLDGLIHVTSNAL 187

Query: 211 SMLVAYDISGNDTAAWTPPRTERDGFWEGGGGGSGL----GFSGGFPSKVAADATVCKDG 270
           SM+V+YD  G++ A+WT P TERDGFWE  G G GL    G + GFPS +  D TVCKDG
Sbjct: 188 SMMVSYDNFGDNVASWTYPATERDGFWEKTGPGLGLDPSTGLNLGFPSGLKEDVTVCKDG 247

Query: 271 AGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGDGMGKTVITC 330
             G Y TVQ+AV+AAPE+   R+FVI I EGVYEE V VP EKKNVVF+GDGMGKTVIT 
Sbjct: 248 KCG-YKTVQDAVNAAPEDNGMRKFVIKISEGVYEENVIVPFEKKNVVFIGDGMGKTVITG 307

Query: 331 SLNVGQPGISTYNTATVGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFI 390
           SLN G PGI+TYNTATVGV+GDGFMA  LT QNTAGPDAHQAVAFRSDSD S+I++CEF+
Sbjct: 308 SLNAGMPGITTYNTATVGVVGDGFMARDLTFQNTAGPDAHQAVAFRSDSDFSLIENCEFL 367

Query: 391 GNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQ 450
           GNQDTLYAH LRQFYK+C+IQGNVDFIFGNSA++FQDCEIL+ PRQ+ PEKGE NA+TAQ
Sbjct: 368 GNQDTLYAHGLRQFYKNCRIQGNVDFIFGNSAAVFQDCEILIAPRQINPEKGEKNAVTAQ 427

Query: 451 GRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRTVFIHCNLEALV 510
           GR DP+QSTGFVF NCLINGT+EYM L+ + PKVHKNFLGRPWK+YSRTVFI CNLEAL+
Sbjct: 428 GRIDPSQSTGFVFLNCLINGTEEYMKLFKANPKVHKNFLGRPWKDYSRTVFIGCNLEALI 487

Query: 511 TPSGWMPWSGDFALKTLFYGEFENSGAGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEW 565
           TP GW+PWSGDFALKTL+YGE +N+G GS  S+RV WSSQIP +HV  YSV NFIQ DEW
Sbjct: 488 TPDGWLPWSGDFALKTLYYGESKNTGPGSDRSQRVSWSSQIPDEHVHVYSVANFIQADEW 547

[Close]

BLAST of Lsi10G007180 vs. ExPASy Swiss-Prot
Match: Q8L7Q7 (Probable pectinesterase/pectinesterase inhibitor 64 OS=Arabidopsis thaliana OX=3702 GN=PME64 PE=2 SV=2)

HSP 1 Score: 641.3 bits (1653), Expect = 1.0e-182
Identity = 332/571 (58.14%), Postives = 414/571 (72.50%), Query Frame = 0


Query: 10  ISKSTLLFLSMASLSFLSFLSLFFFFFLSFSSSAARHRHHLSTHFT-------------N 69
           +S++ +L +  AS   L  +SL  +  +S SS      HH  +H T             +
Sbjct: 31  LSRNGILIIIAASCILLLLISLLIYATVSKSS----RNHHNPSHQTPTSDDHPPPETPPS 90

Query: 70  PPSNDDILHACKATRFPDQCGSSLSR-SSLPPNPTPLQILQSAVALSSDGLATAQSMVNQ 129
           PP    I  AC ATRFPD C +SLS+   +PP+P P+QI+ SA+++S + L + QS +  
Sbjct: 91  PPPIAQIRLACNATRFPDHCVASLSKPGQVPPDPKPVQIIHSAISVSYENLKSGQSKIQS 150

Query: 130 ILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWS 189
           ILDSSA N NRT  A  CLE+L  S +R   T+ A+  G  KDARAW+SA+L Y +DCWS
Sbjct: 151 ILDSSAGNRNRTNIATICLEILSYSQHRTESTDIAVTSGDIKDARAWMSAALAYQFDCWS 210

Query: 190 ALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFWEGG- 249
            LK  NDT+ V  T+ F + L+ L+ NALSM++++D  G+D  +W  P TERDGFWE   
Sbjct: 211 GLKTVNDTKQVVDTITFFEGLVNLTGNALSMMLSFDSFGDDVVSWIRPATERDGFWEKAG 270

Query: 250 ---GGGSGLGFSGGFPSKVAADATVCKDGAGGC-YGTVQEAVDAAPENAAARRFVIHIKE 309
              G G+G   S GFPS +  D TVCK+G   C Y TVQEAVD+AP+     +FVI I+E
Sbjct: 271 PSLGSGTGTEASLGFPSGLTEDVTVCKNGGKDCKYKTVQEAVDSAPDTNRTVKFVIRIRE 330

Query: 310 GVYEEIVRVPLEKKNVVFLGDGMGKTVITCSLNVGQPGISTYNTATVGVLGDGFMASGLT 369
           GVYEE VRVP EKKNVVF+GDGMGKTVIT SLNVGQPG++T+ +ATVGVLGDGFMA  LT
Sbjct: 331 GVYEETVRVPFEKKNVVFIGDGMGKTVITGSLNVGQPGMTTFESATVGVLGDGFMARDLT 390

Query: 370 IQNTAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGN 429
           I+NTAG DAHQAVAFRSDSD SV+++CEF+GNQDTLYAHSLRQFYK C+IQGNVDFIFGN
Sbjct: 391 IENTAGADAHQAVAFRSDSDFSVLENCEFLGNQDTLYAHSLRQFYKQCRIQGNVDFIFGN 450

Query: 430 SASIFQDCEILVRPRQLKPEK-GENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYY 489
           SA++FQDC+IL+  +  K E+ G NNAITA GR D +QSTGFVF NC INGT+EYM  + 
Sbjct: 451 SAAVFQDCDILIASKHSKLEQGGANNAITAHGRIDASQSTGFVFLNCSINGTEEYMKEFQ 510

Query: 490 SKPKVHKNFLGRPWKEYSRTVFIHCNLEALVTPSGWMPWSGDFALKTLFYGEFENSGAGS 549
           + P+ HKNFLGRPWKE+SRTVF++CNLE+L++P GWMPW+GDFALKTL+YGE++N+G GS
Sbjct: 511 ANPEGHKNFLGRPWKEFSRTVFVNCNLESLISPDGWMPWNGDFALKTLYYGEYKNTGPGS 570

Query: 550 KTSERVGWSSQIPAQHVFSYSVQNFIQGDEW 561
             S RV WSS+IP +HV  YSV NFIQ DEW
Sbjct: 571 VRSSRVPWSSEIPEKHVDVYSVANFIQADEW 597

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BLAST of Lsi10G007180 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 7.1e-83
Identity = 200/513 (38.99%), Postives = 287/513 (55.95%), Query Frame = 0


Query: 59  SNDDILHACKATRFPDQCGSSLS-RSSLPPNPTPLQILQSAVALSSDGLATAQSMVNQIL 118
           S +++   C  T  P  C   L+ ++ +          + ++ L+ +   TAQS     L
Sbjct: 24  SPEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISLQLALERATTAQSR-TYTL 83

Query: 119 DSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSAL 178
            S  +N     A ++C E+   +  +++ T+++       D + WLS +L     C ++L
Sbjct: 84  GSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQTWLSTALTNLETCRASL 143

Query: 179 KYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGF--WEGGG 238
                     + +   + ++ L SN ++ L++  +S N      P  + +DGF  W   G
Sbjct: 144 ----------EDLGVPEYVLPLLSNNVTKLISNTLSLNKVPYNEP--SYKDGFPTWVKPG 203

Query: 239 GGSGLGFSGGFPSKVAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEE 298
               L       +   A+  V +DG+G    T+QEAV AA   A   R+VI+IK G Y E
Sbjct: 204 DRKLL------QTTPRANIVVAQDGSGN-VKTIQEAV-AAASRAGGSRYVIYIKAGTYNE 263

Query: 299 IVRVPLEKKNVVFLGDGMGKTVITCSLNVGQPGISTYNTATVGVLGDGFMASGLTIQNTA 358
            + V L  KN++F+GDG+GKT+IT S +VG  G +T+ +ATV V+GD F+A  +TI+NTA
Sbjct: 264 NIEVKL--KNIMFVGDGIGKTIITGSKSVG-GGATTFKSATVAVVGDNFIARDITIRNTA 323

Query: 359 GPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIF 418
           GP+ HQAVA RS SDLSV   C F G QDTLY HS RQFY+ C I G VDFIFGN+A + 
Sbjct: 324 GPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVL 383

Query: 419 QDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVH 478
           Q+C I  R    KP     N +TAQGRTDP QSTG +  NC +    +   +  S     
Sbjct: 384 QNCNIFAR----KP-PNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSV---- 443

Query: 479 KNFLGRPWKEYSRTVFIHCNLEALVTPSGWMPWSGDFALKTLFYGEFENSGAGSKTSERV 538
           K FLGRPWK+YSRTV+I   L++L+ P+GWM WSGDFAL TL+Y E+ N+G GS T+ RV
Sbjct: 444 KTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRV 502

Query: 539 GWSS----QIPAQHVFSYSVQNFIQGDEWIPSS 565
            W        P+Q V  ++V NFI G+ W+P++
Sbjct: 504 KWRGYHVLTSPSQ-VSQFTVGNFIAGNSWLPAT 502

[Close]

BLAST of Lsi10G007180 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 300.4 bits (768), Expect = 4.3e-80
Identity = 194/508 (38.19%), Postives = 281/508 (55.31%), Query Frame = 0


Query: 62  DILHACKATRFPDQCGSSLS-RSSLPPNPTPLQILQSAVALSSDGLATAQSMVNQILDSS 121
           D+   C  T  P  C   L+  S+  P  +  + L+ ++ L  D    A++     L   
Sbjct: 29  DVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHA-FTLGPK 88

Query: 122 AKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYA 181
            ++     A ++C+++   +  +I+ T D   +    DA+ WLS +L     C +     
Sbjct: 89  CRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLEL 148

Query: 182 NDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTA-AWTPPRTERDGFWEGGGGGSG 241
             T +V          + L SN +S L+   ++ N     +TPP  E+DGF      G  
Sbjct: 149 GVTDIV----------LPLMSNNVSNLLCNTLAINKVPFNYTPP--EKDGFPSWVKPGDR 208

Query: 242 LGFSGGFPSKVAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEEIVRV 301
                  P     +A V KDG+G  + T++EA+DAA   + + RFVI++K+GVY E   +
Sbjct: 209 KLLQSSTPKD---NAVVAKDGSGN-FKTIKEAIDAA---SGSGRFVIYVKQGVYSE--NL 268

Query: 302 PLEKKNVVFLGDGMGKTVITCSLNVGQPGISTYNTATVGVLGDGFMASGLTIQNTAGPDA 361
            + KKNV+  GDG+GKT+IT S +VG  G +T+N+ATV  +GDGF+A G+T +NTAG   
Sbjct: 269 EIRKKNVMLRGDGIGKTIITGSKSVG-GGTTTFNSATVAAVGDGFIARGITFRNTAGASN 328

Query: 362 HQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCE 421
            QAVA RS SDLSV   C F   QDTLY HS RQFY+ C + G VDFIFGN+A++ Q+C 
Sbjct: 329 EQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCN 388

Query: 422 ILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFL 481
           I  R    +P + + N ITAQGR+DP Q+TG +  N  +    +   +  S     K +L
Sbjct: 389 IFAR----RP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGST----KTYL 448

Query: 482 GRPWKEYSRTVFIHCNLEALVTPSGWMPWSGDFALKTLFYGEFENSGAGSKTSERVGWSS 541
           GRPW++YSRTVF+  +L++L+ P GW+ W G+FALKTLFY EF+N+G G+ TS RV W  
Sbjct: 449 GRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPG 504

Query: 542 ---QIPAQHVFSYSVQNFIQGDEWIPSS 565
                 A     ++V  F+ G  WIPSS
Sbjct: 509 FRVLGSASEASKFTVGTFLAGGSWIPSS 504

[Close]

BLAST of Lsi10G007180 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 7.3e-80
Identity = 189/517 (36.56%), Postives = 281/517 (54.35%), Query Frame = 0


Query: 67  CKATRFPDQCGSSLSRSSLPPNPTPLQILQSAVALSSDGLATAQSMVNQILDSSAKNLNR 126
           C  T + + C +SL ++S P +  PL +++    ++   +  +    +  L + A N   
Sbjct: 59  CAPTDYKETCVNSLMKAS-PDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKD 118

Query: 127 TTAAKNCLEVLRN---SDYRISLTN-DALR----RGRTKDARAWLSASLLYHYDCWSAL- 186
           T  A    E L N    D +  L N D           +D R WLS S+ Y   C     
Sbjct: 119 TKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFE 178

Query: 187 ----KYANDTQLVDKTMAFLDSL-IGLSSNALSMLVAYDISG--NDTAAWTPP-RTERDG 246
               K + D Q + KT   L S  + + +N  ++L  ++++G   D   +     +  DG
Sbjct: 179 ETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAEDG 238

Query: 247 F--WEGGGGGSGLGFSGGFPSKVAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIH 306
              W G      +   GG    V A+  V  DG+G  Y T+ EA++A P+ A  + FVI+
Sbjct: 239 IPSWVGPNTRRLMATKGG----VKANVVVAHDGSGQ-YKTINEALNAVPK-ANQKPFVIY 298

Query: 307 IKEGVYEEIVRVPLEKKNVVFLGDGMGKTVITCSLNVGQPGISTYNTATVGVLGDGFMAS 366
           IK+GVY E V V  +  +V F+GDG  KT IT SLN     + TY TATV + GD F A 
Sbjct: 299 IKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAK 358

Query: 367 GLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFI 426
            +  +NTAGP+ HQAVA R  +DL+V  +C+  G QDTLY HS RQF++ C + G VDFI
Sbjct: 359 NIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFI 418

Query: 427 FGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSL 486
           FG+   + Q+C I+VR    KP K ++  ITAQGR+D  +STG V QNC I G   Y+ +
Sbjct: 419 FGDGIVVLQNCNIVVR----KPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPV 478

Query: 487 YYSKPKVHKNFLGRPWKEYSRTVFIHCNLEALVTPSGWMPWSGDFALKTLFYGEFENSGA 546
                 ++K +LGRPWKE+SRT+ +   ++ ++ P+GW+PW+GDFAL TL+Y E+EN+G 
Sbjct: 479 ----KSINKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGP 538

Query: 547 GSKTSERVGWS--SQIPAQHVFSYSVQNFIQGDEWIP 563
           GS  ++RV W    ++  +    ++   F++G+ WIP
Sbjct: 539 GSNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIP 560

[Close]

BLAST of Lsi10G007180 vs. ExPASy TrEMBL
Match: A0A1S3CD05 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499643 PE=3 SV=1)

HSP 1 Score: 1073.5 bits (2775), Expect = 2.2e-310
Identity = 546/590 (92.54%), Postives = 561/590 (95.08%), Query Frame = 0


Query: 1   MLPRKPPFPISKSTLLFLSMASLSFLSFLSLFFFFF---LSFSSSAARHRH---HLSTHF 60
           + PRKP FPISKSTLLFLSMASLSFLSFLSL FFFF   LS SSSA RH H   HL +HF
Sbjct: 2   VFPRKPTFPISKSTLLFLSMASLSFLSFLSLSFFFFSLSLSSSSSAVRHHHSHSHLPSHF 61

Query: 61  TNPPSNDDILHACKATRFPDQCGSSLSRSSL-PPNPTPLQILQSAVALSSDGLATAQSMV 120
           TN PS D ILHACK+TRFPDQCGSSLS SS  PPNP+PLQILQSA+ALSSDGL TAQS+V
Sbjct: 62  TNTPS-DQILHACKSTRFPDQCGSSLSHSSXSPPNPSPLQILQSAIALSSDGLTTAQSLV 121

Query: 121 NQILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDC 180
            QILDSSA NLNRTTAAKNCLEVLRNSDYRISLTNDAL RGRTKDARAWLSASLLYHYDC
Sbjct: 122 KQILDSSATNLNRTTAAKNCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDC 181

Query: 181 WSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFWEG 240
           WSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDI GNDTAAWTPPRTERDGFWEG
Sbjct: 182 WSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEG 241

Query: 241 GGGGSGLGFSGGFPSKVAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVY 300
           GGGGSGLGFSGGFP+KV ADATVCKDG+GGCYG++Q+AVDAAPENAAARRFVIHIKEGVY
Sbjct: 242 GGGGSGLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVY 301

Query: 301 EEIVRVPLEKKNVVFLGDGMGKTVITCSLNVGQPGISTYNTATVGVLGDGFMASGLTIQN 360
           EEIVRVPLEKKNVVFLGDGMGKTVIT SLN GQPGISTYNTAT+GVLGDGFMASGLTIQN
Sbjct: 302 EEIVRVPLEKKNVVFLGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQN 361

Query: 361 TAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSAS 420
           TAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSAS
Sbjct: 362 TAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSAS 421

Query: 421 IFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPK 480
           IFQDCEIL+RPRQLKPEKGENNAITA GRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPK
Sbjct: 422 IFQDCEILIRPRQLKPEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPK 481

Query: 481 VHKNFLGRPWKEYSRTVFIHCNLEALVTPSGWMPWSGDFALKTLFYGEFENSGAGSKTSE 540
           VHKNFLGRPWKEYSRTVFIHCNLEALV+PSGWMPWSGDFALKTLFYGEFENSG GSKTSE
Sbjct: 482 VHKNFLGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSE 541

Query: 541 RVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSESSNEQKMLNKKSKDVKLD 584
           RVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSESSN+QKMLNKKSKDVKLD
Sbjct: 542 RVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSESSNDQKMLNKKSKDVKLD 590

[Close]

BLAST of Lsi10G007180 vs. ExPASy TrEMBL
Match: A0A0A0LQ80 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G302130 PE=3 SV=1)

HSP 1 Score: 1064.3 bits (2751), Expect = 1.8e-307
Identity = 539/588 (91.67%), Postives = 561/588 (95.41%), Query Frame = 0


Query: 1   MLPRKPPFPISKSTLLFLSMASLSFLSFLSLFFFF-FLSFSSSAARHRH----HLSTHFT 60
           + PRKP FP SKSTLLFLSMASLSFLSFLSLFFFF  LS SSS+A H H    HL +HFT
Sbjct: 2   VFPRKPTFPFSKSTLLFLSMASLSFLSFLSLFFFFSSLSLSSSSADHHHHPHPHLPSHFT 61

Query: 61  NPPSNDDILHACKATRFPDQCGSSLSRSSLPPNPTPLQILQSAVALSSDGLATAQSMVNQ 120
           N PS D ILHACK+TRFPDQCGSSLS SSLPPNP+PLQILQSA+ALSSDGL TAQS+V Q
Sbjct: 62  NYPS-DQILHACKSTRFPDQCGSSLSHSSLPPNPSPLQILQSAIALSSDGLTTAQSLVKQ 121

Query: 121 ILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWS 180
           ILDSSA NLNRTTAAKNCLEVLRNSDYRISLTNDAL  GRTKDARAWLSASLLYHYDCWS
Sbjct: 122 ILDSSATNLNRTTAAKNCLEVLRNSDYRISLTNDALHHGRTKDARAWLSASLLYHYDCWS 181

Query: 181 ALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFWEGGG 240
           ALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDI GNDTAAWTPPRTERDGFWE GG
Sbjct: 182 ALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWE-GG 241

Query: 241 GGSGLGFSGGFPSKVAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEE 300
           GGSGLGFSGGFP+KV ADATVCKDG+GGCYG++Q+AVDAAPENAAARRFVIHIKEGVYEE
Sbjct: 242 GGSGLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEE 301

Query: 301 IVRVPLEKKNVVFLGDGMGKTVITCSLNVGQPGISTYNTATVGVLGDGFMASGLTIQNTA 360
           IVRVPLEKKNVVFLGDGMGKTVIT SLNVGQPG+STYNTAT+GV+GDGFMASGLTIQNTA
Sbjct: 302 IVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATLGVVGDGFMASGLTIQNTA 361

Query: 361 GPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIF 420
           GPDAHQAVAFRSDSDLSVIQDCEF+GNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIF
Sbjct: 362 GPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIF 421

Query: 421 QDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVH 480
           QDCEILVRPRQLKPEKGENNA+TA GRTDPAQSTGFVFQNCLINGTQEYMSLYYSKP+VH
Sbjct: 422 QDCEILVRPRQLKPEKGENNAVTAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPQVH 481

Query: 481 KNFLGRPWKEYSRTVFIHCNLEALVTPSGWMPWSGDFALKTLFYGEFENSGAGSKTSERV 540
           KNFLGRPWKEYSRTVFIHCNLEALV+PSGWMPWSGDFALKTLFYGEFENSGAGSKTSERV
Sbjct: 482 KNFLGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGAGSKTSERV 541

Query: 541 GWSSQIPAQHVFSYSVQNFIQGDEWIPSSESSNEQKMLNKKSKDVKLD 584
           GWSSQIPAQHVFSYSV+NFIQGDEWIPSSESS+EQKMLNK+SKDVKLD
Sbjct: 542 GWSSQIPAQHVFSYSVENFIQGDEWIPSSESSDEQKMLNKRSKDVKLD 587

[Close]

BLAST of Lsi10G007180 vs. ExPASy TrEMBL
Match: A0A5A7TBR9 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold680G00730 PE=3 SV=1)

HSP 1 Score: 1010.4 bits (2611), Expect = 3.1e-291
Identity = 497/521 (95.39%), Postives = 510/521 (97.89%), Query Frame = 0


Query: 63  ILHACKATRFPDQCGSSLSRSSLPPNPTPLQILQSAVALSSDGLATAQSMVNQILDSSAK 122
           ILHACK+TRFPDQCGSSLS SSLPPNP+PLQILQSA+ALSSDGL TAQS+V QILDSSA 
Sbjct: 42  ILHACKSTRFPDQCGSSLSHSSLPPNPSPLQILQSAIALSSDGLTTAQSLVKQILDSSAT 101

Query: 123 NLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYAND 182
           NLNRTTAAKNCLEVLRNSDYRISLTNDAL RGRTKDARAWLSASLLYHYDCWSALKYAND
Sbjct: 102 NLNRTTAAKNCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALKYAND 161

Query: 183 TQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFWEGGGGGSGLGF 242
           TQLVDKTMAFLDSLIGLSSNALSMLVAYDI GNDTAAWTPPRTERDGFWEGGGGGSGLGF
Sbjct: 162 TQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGGSGLGF 221

Query: 243 SGGFPSKVAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEEIVRVPLE 302
           SGGFP+KV ADATVCKDG+GGCYG++Q+AVDAAPENAAARRFVIHIKEGVYEEIVRVPLE
Sbjct: 222 SGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLE 281

Query: 303 KKNVVFLGDGMGKTVITCSLNVGQPGISTYNTATVGVLGDGFMASGLTIQNTAGPDAHQA 362
           KKNVVFLGDGMGKTVIT SLN GQPGISTYNTAT+GVLGDGFMASGLTIQNTAGPDAHQA
Sbjct: 282 KKNVVFLGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQA 341

Query: 363 VAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILV 422
           VAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEIL+
Sbjct: 342 VAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILI 401

Query: 423 RPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRP 482
           RPRQLKPEKGENNAITA GRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRP
Sbjct: 402 RPRQLKPEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRP 461

Query: 483 WKEYSRTVFIHCNLEALVTPSGWMPWSGDFALKTLFYGEFENSGAGSKTSERVGWSSQIP 542
           WKEYSRTVFIHCNLEALV+PSGWMPWSGDFALKTLFYGEFENSG GSKTSERVGWSSQIP
Sbjct: 462 WKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQIP 521

Query: 543 AQHVFSYSVQNFIQGDEWIPSSESSNEQKMLNKKSKDVKLD 584
           AQHVFSYSVQNFIQGDEWIPSSESSN+QKMLNKKSKDVKLD
Sbjct: 522 AQHVFSYSVQNFIQGDEWIPSSESSNDQKMLNKKSKDVKLD 562

[Close]

BLAST of Lsi10G007180 vs. ExPASy TrEMBL
Match: A0A6J1EV58 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111438335 PE=3 SV=1)

HSP 1 Score: 993.8 bits (2568), Expect = 3.0e-286
Identity = 496/580 (85.52%), Postives = 532/580 (91.72%), Query Frame = 0


Query: 2   LPRKPPFPI------SKSTLLFLSMASLSFLSFLSLFFFFFLSFSSSAARHRHHLSTHFT 61
           LPR  PFP       +KST LFLSMA+LSFLSFLSLFFF  LS +     H HHL +HFT
Sbjct: 5   LPRTTPFPFPFQFNAAKSTFLFLSMAALSFLSFLSLFFFLSLSSAVHHRHHHHHLPSHFT 64

Query: 62  NPPSNDDILHACKATRFPDQCGSSLSRS-SLPPNPTPLQILQSAVALSSDGLATAQSMVN 121
           N PS+++   ACKATRFPDQCGSSLS S SLPPNPT LQI+QSA+A+SSDGLA+A+SMV 
Sbjct: 65  NSPSSEETFRACKATRFPDQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKSMVK 124

Query: 122 QILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCW 181
           QILDSS  + NRTTAAKNCLEVLR S+YRISLTNDA+RRGRTKDARAW+SASLLYHYDCW
Sbjct: 125 QILDSSTDSPNRTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYHYDCW 184

Query: 182 SALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFWEGG 241
           SALKYANDTQ V+KTMAFLDSLIGL+SNALSML AYDISGNDTAAWTPPRTERDGFWEG 
Sbjct: 185 SALKYANDTQSVNKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTPPRTERDGFWEGS 244

Query: 242 GGGSGLGFSGGFPSKVAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYE 301
            GGSGLGFSGGFPSKV A ATVCKDGAGGCY TVQEAV+AAP+NA  +RFVIHIKEGVY+
Sbjct: 245 SGGSGLGFSGGFPSKVDASATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKEGVYD 304

Query: 302 EIVRVPLEKKNVVFLGDGMGKTVITCSLNVGQPGISTYNTATVGVLGDGFMASGLTIQNT 361
           EIVRVPLEKKNVVFLGDGMGKTVIT SLNVGQPGISTYNTATVGV+GDGFMASGLTIQNT
Sbjct: 305 EIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLTIQNT 364

Query: 362 AGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASI 421
           AGPDAHQAVAFRSDSDLSVIQDCEF+GNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSAS+
Sbjct: 365 AGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASV 424

Query: 422 FQDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKV 481
           FQDCEILVRPRQLKPEKGENNA+TA GRTDPAQ+TGFVFQNCL+NGTQEYM+LYYSKPKV
Sbjct: 425 FQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQTTGFVFQNCLVNGTQEYMALYYSKPKV 484

Query: 482 HKNFLGRPWKEYSRTVFIHCNLEALVTPSGWMPWSGDFALKTLFYGEFENSGAGSKTSER 541
           HKNFLGRPWKEYSRTVFIHCNLEALV+P+GWMPW+GDFALKTL+YGEFENSG GSKTSER
Sbjct: 485 HKNFLGRPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSKTSER 544

Query: 542 VGWSSQIPAQHVFSYSVQNFIQGDEWIPSSESSNEQKMLN 575
           V WSSQIPAQHV+SYSVQNFIQGDEWIPSS+SS E K +N
Sbjct: 545 VEWSSQIPAQHVYSYSVQNFIQGDEWIPSSQSSEESKTIN 584

[Close]

BLAST of Lsi10G007180 vs. ExPASy TrEMBL
Match: A0A6J1I3S8 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470304 PE=3 SV=1)

HSP 1 Score: 980.7 bits (2534), Expect = 2.6e-282
Identity = 493/580 (85.00%), Postives = 531/580 (91.55%), Query Frame = 0


Query: 2   LPRKPPFPI------SKSTLLFLSMASLSFLSFLSLFFFFFLSFSSSAARHRHHLSTHFT 61
           LPR  PFP       +KST LFLSMA+LSFLSFL L  FFFLS SS+     HHL +HFT
Sbjct: 5   LPRTTPFPFLFQFTTAKSTFLFLSMAALSFLSFLCL--FFFLSLSSAVHHRHHHLPSHFT 64

Query: 62  NPPSNDDILHACKATRFPDQCGSSLSRS-SLPPNPTPLQILQSAVALSSDGLATAQSMVN 121
           N PS+++ L ACKATRFP+QCGSSLS S SLPPNPT LQI+QSA+A+SSDGLA+A+SMV 
Sbjct: 65  NFPSSEETLRACKATRFPNQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKSMVK 124

Query: 122 QILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCW 181
           +ILDSS  + NRTTAAKNCLEVLR S+YRISLTNDA+RRGRTKDARAW+SASLLYHYDCW
Sbjct: 125 KILDSSTDSPNRTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYHYDCW 184

Query: 182 SALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFWEGG 241
           SALKYANDTQ V+KTMAFLDSLIGL+SNALSML AYDISGNDTAAWTPPRTERDGFWEG 
Sbjct: 185 SALKYANDTQSVNKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTPPRTERDGFWEGS 244

Query: 242 GGGSGLGFSGGFPSKVAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYE 301
            GGSGLGFSGGFPSKV A ATVCKDGAGGCY TVQEAV+AAP+NA  +RFVIHIKEGVY+
Sbjct: 245 SGGSGLGFSGGFPSKVDASATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKEGVYD 304

Query: 302 EIVRVPLEKKNVVFLGDGMGKTVITCSLNVGQPGISTYNTATVGVLGDGFMASGLTIQNT 361
           EIVRVPLEKKNVVFLGDGMGKTVIT SLNVGQPGISTYN+ATVGV+GDGFMASGLTIQNT
Sbjct: 305 EIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNSATVGVVGDGFMASGLTIQNT 364

Query: 362 AGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASI 421
           AGPDAHQAVAFRSDSDLSVIQDCEF+GNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSAS+
Sbjct: 365 AGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASV 424

Query: 422 FQDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKV 481
           FQDCEILV PRQLKPEKGENNA+TA GRTDPAQ+TGFVFQNCLINGTQEYM+LYYSKPKV
Sbjct: 425 FQDCEILVCPRQLKPEKGENNAVTAHGRTDPAQTTGFVFQNCLINGTQEYMALYYSKPKV 484

Query: 482 HKNFLGRPWKEYSRTVFIHCNLEALVTPSGWMPWSGDFALKTLFYGEFENSGAGSKTSER 541
           HKNFLGRPWKEYSRTVFIHCNLEALV+P+GWMPW+GDFALKTL+YGEFENSG GS TSER
Sbjct: 485 HKNFLGRPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSITSER 544

Query: 542 VGWSSQIPAQHVFSYSVQNFIQGDEWIPSSESSNEQKMLN 575
           V WSSQIPAQHV+SYSVQNFIQGDEWIPSS+SS E K +N
Sbjct: 545 VEWSSQIPAQHVYSYSVQNFIQGDEWIPSSQSSEESKTIN 582

[Close]

BLAST of Lsi10G007180 vs. NCBI nr
Match: XP_038902126.1 (probable pectinesterase/pectinesterase inhibitor 51 [Benincasa hispida])

HSP 1 Score: 1099.3 bits (2842), Expect = 0.0e+00
Identity = 555/583 (95.20%), Postives = 566/583 (97.08%), Query Frame = 0


Query: 1   MLPRKPPFPISKSTLLFLSMASLSFLSFLSLFFFFFLSFSSSAARHRHHLSTHFTNPPSN 60
           M+PRK  FPISKSTLLFLSMASLSFLSFLSL F FFLS SS+A    HHLSTHFTNPPS 
Sbjct: 1   MVPRKTHFPISKSTLLFLSMASLSFLSFLSL-FSFFLSLSSAARHRHHHLSTHFTNPPSI 60

Query: 61  DDILHACKATRFPDQCGSSLSRSSLPPNPTPLQILQSAVALSSDGLATAQSMVNQILDSS 120
           D ILHACKATRFPDQCGSSLS SSLPPNP+PLQILQSAV+LSSDGL+TAQSMV QILDSS
Sbjct: 61  DGILHACKATRFPDQCGSSLSHSSLPPNPSPLQILQSAVSLSSDGLSTAQSMVKQILDSS 120

Query: 121 AKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYA 180
           AKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYA
Sbjct: 121 AKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYA 180

Query: 181 NDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFWEGGGGGSGL 240
           NDTQLVDKTMAFLDSLIGLSSNALSMLVAYDI GNDTAAWTPPRTERDGFWEGGGGGSGL
Sbjct: 181 NDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGGSGL 240

Query: 241 GFSGGFPSKVAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEEIVRVP 300
           GFSGGFPSK+AADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEEIVRVP
Sbjct: 241 GFSGGFPSKMAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEEIVRVP 300

Query: 301 LEKKNVVFLGDGMGKTVITCSLNVGQPGISTYNTATVGVLGDGFMASGLTIQNTAGPDAH 360
           LEKKNVVFLGDGMGKTVIT SLNVGQPG+STYNTATVGVLGDGFMASGLTIQNTAGPDAH
Sbjct: 301 LEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATVGVLGDGFMASGLTIQNTAGPDAH 360

Query: 361 QAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEI 420
           QAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEI
Sbjct: 361 QAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEI 420

Query: 421 LVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLG 480
           LVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLG
Sbjct: 421 LVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLG 480

Query: 481 RPWKEYSRTVFIHCNLEALVTPSGWMPWSGDFALKTLFYGEFENSGAGSKTSERVGWSSQ 540
           RPWKEYSRTVFIHCNLE+LVTP+GWMPWSGDFALKTLFYGEFENSG GSKTSERVGWSSQ
Sbjct: 481 RPWKEYSRTVFIHCNLESLVTPAGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQ 540

Query: 541 IPAQHVFSYSVQNFIQGDEWIPSSESSNEQKMLNKKSKDVKLD 584
           IPAQHVFSYSVQNFIQGDEWIPSS++SNE KMLNKKSKDVKLD
Sbjct: 541 IPAQHVFSYSVQNFIQGDEWIPSSQTSNEPKMLNKKSKDVKLD 582

[Close]

BLAST of Lsi10G007180 vs. NCBI nr
Match: XP_008460902.1 (PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor 51 [Cucumis melo])

HSP 1 Score: 1073.5 bits (2775), Expect = 4.5e-310
Identity = 546/590 (92.54%), Postives = 561/590 (95.08%), Query Frame = 0


Query: 1   MLPRKPPFPISKSTLLFLSMASLSFLSFLSLFFFFF---LSFSSSAARHRH---HLSTHF 60
           + PRKP FPISKSTLLFLSMASLSFLSFLSL FFFF   LS SSSA RH H   HL +HF
Sbjct: 2   VFPRKPTFPISKSTLLFLSMASLSFLSFLSLSFFFFSLSLSSSSSAVRHHHSHSHLPSHF 61

Query: 61  TNPPSNDDILHACKATRFPDQCGSSLSRSSL-PPNPTPLQILQSAVALSSDGLATAQSMV 120
           TN PS D ILHACK+TRFPDQCGSSLS SS  PPNP+PLQILQSA+ALSSDGL TAQS+V
Sbjct: 62  TNTPS-DQILHACKSTRFPDQCGSSLSHSSXSPPNPSPLQILQSAIALSSDGLTTAQSLV 121

Query: 121 NQILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDC 180
            QILDSSA NLNRTTAAKNCLEVLRNSDYRISLTNDAL RGRTKDARAWLSASLLYHYDC
Sbjct: 122 KQILDSSATNLNRTTAAKNCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDC 181

Query: 181 WSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFWEG 240
           WSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDI GNDTAAWTPPRTERDGFWEG
Sbjct: 182 WSALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEG 241

Query: 241 GGGGSGLGFSGGFPSKVAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVY 300
           GGGGSGLGFSGGFP+KV ADATVCKDG+GGCYG++Q+AVDAAPENAAARRFVIHIKEGVY
Sbjct: 242 GGGGSGLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVY 301

Query: 301 EEIVRVPLEKKNVVFLGDGMGKTVITCSLNVGQPGISTYNTATVGVLGDGFMASGLTIQN 360
           EEIVRVPLEKKNVVFLGDGMGKTVIT SLN GQPGISTYNTAT+GVLGDGFMASGLTIQN
Sbjct: 302 EEIVRVPLEKKNVVFLGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQN 361

Query: 361 TAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSAS 420
           TAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSAS
Sbjct: 362 TAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSAS 421

Query: 421 IFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPK 480
           IFQDCEIL+RPRQLKPEKGENNAITA GRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPK
Sbjct: 422 IFQDCEILIRPRQLKPEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPK 481

Query: 481 VHKNFLGRPWKEYSRTVFIHCNLEALVTPSGWMPWSGDFALKTLFYGEFENSGAGSKTSE 540
           VHKNFLGRPWKEYSRTVFIHCNLEALV+PSGWMPWSGDFALKTLFYGEFENSG GSKTSE
Sbjct: 482 VHKNFLGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSE 541

Query: 541 RVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSESSNEQKMLNKKSKDVKLD 584
           RVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSESSN+QKMLNKKSKDVKLD
Sbjct: 542 RVGWSSQIPAQHVFSYSVQNFIQGDEWIPSSESSNDQKMLNKKSKDVKLD 590

[Close]

BLAST of Lsi10G007180 vs. NCBI nr
Match: XP_004147375.2 (probable pectinesterase/pectinesterase inhibitor 51 [Cucumis sativus] >KGN62146.1 hypothetical protein Csa_005971 [Cucumis sativus])

HSP 1 Score: 1064.3 bits (2751), Expect = 3.7e-307
Identity = 539/588 (91.67%), Postives = 561/588 (95.41%), Query Frame = 0


Query: 1   MLPRKPPFPISKSTLLFLSMASLSFLSFLSLFFFF-FLSFSSSAARHRH----HLSTHFT 60
           + PRKP FP SKSTLLFLSMASLSFLSFLSLFFFF  LS SSS+A H H    HL +HFT
Sbjct: 2   VFPRKPTFPFSKSTLLFLSMASLSFLSFLSLFFFFSSLSLSSSSADHHHHPHPHLPSHFT 61

Query: 61  NPPSNDDILHACKATRFPDQCGSSLSRSSLPPNPTPLQILQSAVALSSDGLATAQSMVNQ 120
           N PS D ILHACK+TRFPDQCGSSLS SSLPPNP+PLQILQSA+ALSSDGL TAQS+V Q
Sbjct: 62  NYPS-DQILHACKSTRFPDQCGSSLSHSSLPPNPSPLQILQSAIALSSDGLTTAQSLVKQ 121

Query: 121 ILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWS 180
           ILDSSA NLNRTTAAKNCLEVLRNSDYRISLTNDAL  GRTKDARAWLSASLLYHYDCWS
Sbjct: 122 ILDSSATNLNRTTAAKNCLEVLRNSDYRISLTNDALHHGRTKDARAWLSASLLYHYDCWS 181

Query: 181 ALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFWEGGG 240
           ALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDI GNDTAAWTPPRTERDGFWE GG
Sbjct: 182 ALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWE-GG 241

Query: 241 GGSGLGFSGGFPSKVAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEE 300
           GGSGLGFSGGFP+KV ADATVCKDG+GGCYG++Q+AVDAAPENAAARRFVIHIKEGVYEE
Sbjct: 242 GGSGLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEE 301

Query: 301 IVRVPLEKKNVVFLGDGMGKTVITCSLNVGQPGISTYNTATVGVLGDGFMASGLTIQNTA 360
           IVRVPLEKKNVVFLGDGMGKTVIT SLNVGQPG+STYNTAT+GV+GDGFMASGLTIQNTA
Sbjct: 302 IVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATLGVVGDGFMASGLTIQNTA 361

Query: 361 GPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIF 420
           GPDAHQAVAFRSDSDLSVIQDCEF+GNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIF
Sbjct: 362 GPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIF 421

Query: 421 QDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVH 480
           QDCEILVRPRQLKPEKGENNA+TA GRTDPAQSTGFVFQNCLINGTQEYMSLYYSKP+VH
Sbjct: 422 QDCEILVRPRQLKPEKGENNAVTAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPQVH 481

Query: 481 KNFLGRPWKEYSRTVFIHCNLEALVTPSGWMPWSGDFALKTLFYGEFENSGAGSKTSERV 540
           KNFLGRPWKEYSRTVFIHCNLEALV+PSGWMPWSGDFALKTLFYGEFENSGAGSKTSERV
Sbjct: 482 KNFLGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGAGSKTSERV 541

Query: 541 GWSSQIPAQHVFSYSVQNFIQGDEWIPSSESSNEQKMLNKKSKDVKLD 584
           GWSSQIPAQHVFSYSV+NFIQGDEWIPSSESS+EQKMLNK+SKDVKLD
Sbjct: 542 GWSSQIPAQHVFSYSVENFIQGDEWIPSSESSDEQKMLNKRSKDVKLD 587

[Close]

BLAST of Lsi10G007180 vs. NCBI nr
Match: KAA0040770.1 (putative pectinesterase/pectinesterase inhibitor 51 [Cucumis melo var. makuwa] >TYK02086.1 putative pectinesterase/pectinesterase inhibitor 51 [Cucumis melo var. makuwa])

HSP 1 Score: 1010.4 bits (2611), Expect = 6.4e-291
Identity = 497/521 (95.39%), Postives = 510/521 (97.89%), Query Frame = 0


Query: 63  ILHACKATRFPDQCGSSLSRSSLPPNPTPLQILQSAVALSSDGLATAQSMVNQILDSSAK 122
           ILHACK+TRFPDQCGSSLS SSLPPNP+PLQILQSA+ALSSDGL TAQS+V QILDSSA 
Sbjct: 42  ILHACKSTRFPDQCGSSLSHSSLPPNPSPLQILQSAIALSSDGLTTAQSLVKQILDSSAT 101

Query: 123 NLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYAND 182
           NLNRTTAAKNCLEVLRNSDYRISLTNDAL RGRTKDARAWLSASLLYHYDCWSALKYAND
Sbjct: 102 NLNRTTAAKNCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALKYAND 161

Query: 183 TQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFWEGGGGGSGLGF 242
           TQLVDKTMAFLDSLIGLSSNALSMLVAYDI GNDTAAWTPPRTERDGFWEGGGGGSGLGF
Sbjct: 162 TQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGGSGLGF 221

Query: 243 SGGFPSKVAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEEIVRVPLE 302
           SGGFP+KV ADATVCKDG+GGCYG++Q+AVDAAPENAAARRFVIHIKEGVYEEIVRVPLE
Sbjct: 222 SGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPLE 281

Query: 303 KKNVVFLGDGMGKTVITCSLNVGQPGISTYNTATVGVLGDGFMASGLTIQNTAGPDAHQA 362
           KKNVVFLGDGMGKTVIT SLN GQPGISTYNTAT+GVLGDGFMASGLTIQNTAGPDAHQA
Sbjct: 282 KKNVVFLGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQA 341

Query: 363 VAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILV 422
           VAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEIL+
Sbjct: 342 VAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILI 401

Query: 423 RPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRP 482
           RPRQLKPEKGENNAITA GRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRP
Sbjct: 402 RPRQLKPEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRP 461

Query: 483 WKEYSRTVFIHCNLEALVTPSGWMPWSGDFALKTLFYGEFENSGAGSKTSERVGWSSQIP 542
           WKEYSRTVFIHCNLEALV+PSGWMPWSGDFALKTLFYGEFENSG GSKTSERVGWSSQIP
Sbjct: 462 WKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQIP 521

Query: 543 AQHVFSYSVQNFIQGDEWIPSSESSNEQKMLNKKSKDVKLD 584
           AQHVFSYSVQNFIQGDEWIPSSESSN+QKMLNKKSKDVKLD
Sbjct: 522 AQHVFSYSVQNFIQGDEWIPSSESSNDQKMLNKKSKDVKLD 562

[Close]

BLAST of Lsi10G007180 vs. NCBI nr
Match: XP_022932006.1 (probable pectinesterase/pectinesterase inhibitor 51 [Cucurbita moschata])

HSP 1 Score: 993.8 bits (2568), Expect = 6.2e-286
Identity = 496/580 (85.52%), Postives = 532/580 (91.72%), Query Frame = 0


Query: 2   LPRKPPFPI------SKSTLLFLSMASLSFLSFLSLFFFFFLSFSSSAARHRHHLSTHFT 61
           LPR  PFP       +KST LFLSMA+LSFLSFLSLFFF  LS +     H HHL +HFT
Sbjct: 5   LPRTTPFPFPFQFNAAKSTFLFLSMAALSFLSFLSLFFFLSLSSAVHHRHHHHHLPSHFT 64

Query: 62  NPPSNDDILHACKATRFPDQCGSSLSRS-SLPPNPTPLQILQSAVALSSDGLATAQSMVN 121
           N PS+++   ACKATRFPDQCGSSLS S SLPPNPT LQI+QSA+A+SSDGLA+A+SMV 
Sbjct: 65  NSPSSEETFRACKATRFPDQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKSMVK 124

Query: 122 QILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCW 181
           QILDSS  + NRTTAAKNCLEVLR S+YRISLTNDA+RRGRTKDARAW+SASLLYHYDCW
Sbjct: 125 QILDSSTDSPNRTTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYHYDCW 184

Query: 182 SALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFWEGG 241
           SALKYANDTQ V+KTMAFLDSLIGL+SNALSML AYDISGNDTAAWTPPRTERDGFWEG 
Sbjct: 185 SALKYANDTQSVNKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTPPRTERDGFWEGS 244

Query: 242 GGGSGLGFSGGFPSKVAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYE 301
            GGSGLGFSGGFPSKV A ATVCKDGAGGCY TVQEAV+AAP+NA  +RFVIHIKEGVY+
Sbjct: 245 SGGSGLGFSGGFPSKVDASATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKEGVYD 304

Query: 302 EIVRVPLEKKNVVFLGDGMGKTVITCSLNVGQPGISTYNTATVGVLGDGFMASGLTIQNT 361
           EIVRVPLEKKNVVFLGDGMGKTVIT SLNVGQPGISTYNTATVGV+GDGFMASGLTIQNT
Sbjct: 305 EIVRVPLEKKNVVFLGDGMGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLTIQNT 364

Query: 362 AGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASI 421
           AGPDAHQAVAFRSDSDLSVIQDCEF+GNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSAS+
Sbjct: 365 AGPDAHQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASV 424

Query: 422 FQDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKV 481
           FQDCEILVRPRQLKPEKGENNA+TA GRTDPAQ+TGFVFQNCL+NGTQEYM+LYYSKPKV
Sbjct: 425 FQDCEILVRPRQLKPEKGENNAVTAHGRTDPAQTTGFVFQNCLVNGTQEYMALYYSKPKV 484

Query: 482 HKNFLGRPWKEYSRTVFIHCNLEALVTPSGWMPWSGDFALKTLFYGEFENSGAGSKTSER 541
           HKNFLGRPWKEYSRTVFIHCNLEALV+P+GWMPW+GDFALKTL+YGEFENSG GSKTSER
Sbjct: 485 HKNFLGRPWKEYSRTVFIHCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSKTSER 544

Query: 542 VGWSSQIPAQHVFSYSVQNFIQGDEWIPSSESSNEQKMLN 575
           V WSSQIPAQHV+SYSVQNFIQGDEWIPSS+SS E K +N
Sbjct: 545 VEWSSQIPAQHVYSYSVQNFIQGDEWIPSSQSSEESKTIN 584

[Close]

BLAST of Lsi10G007180 vs. TAIR 10
Match: AT5G09760.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 714.9 bits (1844), Expect = 5.2e-206
Identity = 361/544 (66.36%), Postives = 427/544 (78.49%), Query Frame = 0


Query: 31  LFFFFFLSFSSSAARHRHHLSTHFTNP--PSND---DILHACKATRFPDQCGSSLS-RSS 90
           LF  F  S  SS++RH HH ++  T+P  PS+     I  AC ATR+PDQC SSLS +  
Sbjct: 8   LFSLFLFSSPSSSSRHHHHHNSGDTSPVNPSSSLAAQIRLACNATRYPDQCVSSLSEQGR 67

Query: 91  LPPNPTPLQILQSAVALSSDGLATAQSMVNQILDSSAKNLNRTTAAKNCLEVLRNSDYRI 150
           +PP+P P+QI+ SA++ S   L TAQS +  I+DSS  NLNRT AA  CL++L  S++R 
Sbjct: 68  VPPDPKPIQIIHSAISFSFQNLKTAQSKIKSIVDSSVGNLNRTNAANTCLQLLTYSEHRT 127

Query: 151 SLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYANDTQLVDKTMAFLDSLIGLSSNAL 210
             T+ AL RG+ KDARAW+SA+L+Y YD WSALKY NDT  V +TM+FLD LI ++SNAL
Sbjct: 128 QSTDQALTRGKIKDARAWMSAALVYQYDSWSALKYVNDTSQVGETMSFLDGLIHVTSNAL 187

Query: 211 SMLVAYDISGNDTAAWTPPRTERDGFWEGGGGGSGL----GFSGGFPSKVAADATVCKDG 270
           SM+V+YD  G++ A+WT P TERDGFWE  G G GL    G + GFPS +  D TVCKDG
Sbjct: 188 SMMVSYDNFGDNVASWTYPATERDGFWEKTGPGLGLDPSTGLNLGFPSGLKEDVTVCKDG 247

Query: 271 AGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKKNVVFLGDGMGKTVITC 330
             G Y TVQ+AV+AAPE+   R+FVI I EGVYEE V VP EKKNVVF+GDGMGKTVIT 
Sbjct: 248 KCG-YKTVQDAVNAAPEDNGMRKFVIKISEGVYEENVIVPFEKKNVVFIGDGMGKTVITG 307

Query: 331 SLNVGQPGISTYNTATVGVLGDGFMASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFI 390
           SLN G PGI+TYNTATVGV+GDGFMA  LT QNTAGPDAHQAVAFRSDSD S+I++CEF+
Sbjct: 308 SLNAGMPGITTYNTATVGVVGDGFMARDLTFQNTAGPDAHQAVAFRSDSDFSLIENCEFL 367

Query: 391 GNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQ 450
           GNQDTLYAH LRQFYK+C+IQGNVDFIFGNSA++FQDCEIL+ PRQ+ PEKGE NA+TAQ
Sbjct: 368 GNQDTLYAHGLRQFYKNCRIQGNVDFIFGNSAAVFQDCEILIAPRQINPEKGEKNAVTAQ 427

Query: 451 GRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWKEYSRTVFIHCNLEALV 510
           GR DP+QSTGFVF NCLINGT+EYM L+ + PKVHKNFLGRPWK+YSRTVFI CNLEAL+
Sbjct: 428 GRIDPSQSTGFVFLNCLINGTEEYMKLFKANPKVHKNFLGRPWKDYSRTVFIGCNLEALI 487

Query: 511 TPSGWMPWSGDFALKTLFYGEFENSGAGSKTSERVGWSSQIPAQHVFSYSVQNFIQGDEW 565
           TP GW+PWSGDFALKTL+YGE +N+G GS  S+RV WSSQIP +HV  YSV NFIQ DEW
Sbjct: 488 TPDGWLPWSGDFALKTLYYGESKNTGPGSDRSQRVSWSSQIPDEHVHVYSVANFIQADEW 547

[Close]

BLAST of Lsi10G007180 vs. TAIR 10
Match: AT5G64640.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 641.3 bits (1653), Expect = 7.3e-184
Identity = 332/571 (58.14%), Postives = 414/571 (72.50%), Query Frame = 0


Query: 10  ISKSTLLFLSMASLSFLSFLSLFFFFFLSFSSSAARHRHHLSTHFT-------------N 69
           +S++ +L +  AS   L  +SL  +  +S SS      HH  +H T             +
Sbjct: 31  LSRNGILIIIAASCILLLLISLLIYATVSKSS----RNHHNPSHQTPTSDDHPPPETPPS 90

Query: 70  PPSNDDILHACKATRFPDQCGSSLSR-SSLPPNPTPLQILQSAVALSSDGLATAQSMVNQ 129
           PP    I  AC ATRFPD C +SLS+   +PP+P P+QI+ SA+++S + L + QS +  
Sbjct: 91  PPPIAQIRLACNATRFPDHCVASLSKPGQVPPDPKPVQIIHSAISVSYENLKSGQSKIQS 150

Query: 130 ILDSSAKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWS 189
           ILDSSA N NRT  A  CLE+L  S +R   T+ A+  G  KDARAW+SA+L Y +DCWS
Sbjct: 151 ILDSSAGNRNRTNIATICLEILSYSQHRTESTDIAVTSGDIKDARAWMSAALAYQFDCWS 210

Query: 190 ALKYANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTAAWTPPRTERDGFWEGG- 249
            LK  NDT+ V  T+ F + L+ L+ NALSM++++D  G+D  +W  P TERDGFWE   
Sbjct: 211 GLKTVNDTKQVVDTITFFEGLVNLTGNALSMMLSFDSFGDDVVSWIRPATERDGFWEKAG 270

Query: 250 ---GGGSGLGFSGGFPSKVAADATVCKDGAGGC-YGTVQEAVDAAPENAAARRFVIHIKE 309
              G G+G   S GFPS +  D TVCK+G   C Y TVQEAVD+AP+     +FVI I+E
Sbjct: 271 PSLGSGTGTEASLGFPSGLTEDVTVCKNGGKDCKYKTVQEAVDSAPDTNRTVKFVIRIRE 330

Query: 310 GVYEEIVRVPLEKKNVVFLGDGMGKTVITCSLNVGQPGISTYNTATVGVLGDGFMASGLT 369
           GVYEE VRVP EKKNVVF+GDGMGKTVIT SLNVGQPG++T+ +ATVGVLGDGFMA  LT
Sbjct: 331 GVYEETVRVPFEKKNVVFIGDGMGKTVITGSLNVGQPGMTTFESATVGVLGDGFMARDLT 390

Query: 370 IQNTAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGN 429
           I+NTAG DAHQAVAFRSDSD SV+++CEF+GNQDTLYAHSLRQFYK C+IQGNVDFIFGN
Sbjct: 391 IENTAGADAHQAVAFRSDSDFSVLENCEFLGNQDTLYAHSLRQFYKQCRIQGNVDFIFGN 450

Query: 430 SASIFQDCEILVRPRQLKPEK-GENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYY 489
           SA++FQDC+IL+  +  K E+ G NNAITA GR D +QSTGFVF NC INGT+EYM  + 
Sbjct: 451 SAAVFQDCDILIASKHSKLEQGGANNAITAHGRIDASQSTGFVFLNCSINGTEEYMKEFQ 510

Query: 490 SKPKVHKNFLGRPWKEYSRTVFIHCNLEALVTPSGWMPWSGDFALKTLFYGEFENSGAGS 549
           + P+ HKNFLGRPWKE+SRTVF++CNLE+L++P GWMPW+GDFALKTL+YGE++N+G GS
Sbjct: 511 ANPEGHKNFLGRPWKEFSRTVFVNCNLESLISPDGWMPWNGDFALKTLYYGEYKNTGPGS 570

Query: 550 KTSERVGWSSQIPAQHVFSYSVQNFIQGDEW 561
             S RV WSS+IP +HV  YSV NFIQ DEW
Sbjct: 571 VRSSRVPWSSEIPEKHVDVYSVANFIQADEW 597

[Close]

BLAST of Lsi10G007180 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 300.4 bits (768), Expect = 3.1e-81
Identity = 194/508 (38.19%), Postives = 281/508 (55.31%), Query Frame = 0


Query: 62  DILHACKATRFPDQCGSSLS-RSSLPPNPTPLQILQSAVALSSDGLATAQSMVNQILDSS 121
           D+   C  T  P  C   L+  S+  P  +  + L+ ++ L  D    A++     L   
Sbjct: 29  DVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHA-FTLGPK 88

Query: 122 AKNLNRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYA 181
            ++     A ++C+++   +  +I+ T D   +    DA+ WLS +L     C +     
Sbjct: 89  CRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLEL 148

Query: 182 NDTQLVDKTMAFLDSLIGLSSNALSMLVAYDISGNDTA-AWTPPRTERDGFWEGGGGGSG 241
             T +V          + L SN +S L+   ++ N     +TPP  E+DGF      G  
Sbjct: 149 GVTDIV----------LPLMSNNVSNLLCNTLAINKVPFNYTPP--EKDGFPSWVKPGDR 208

Query: 242 LGFSGGFPSKVAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEEIVRV 301
                  P     +A V KDG+G  + T++EA+DAA   + + RFVI++K+GVY E   +
Sbjct: 209 KLLQSSTPKD---NAVVAKDGSGN-FKTIKEAIDAA---SGSGRFVIYVKQGVYSE--NL 268

Query: 302 PLEKKNVVFLGDGMGKTVITCSLNVGQPGISTYNTATVGVLGDGFMASGLTIQNTAGPDA 361
            + KKNV+  GDG+GKT+IT S +VG  G +T+N+ATV  +GDGF+A G+T +NTAG   
Sbjct: 269 EIRKKNVMLRGDGIGKTIITGSKSVG-GGTTTFNSATVAAVGDGFIARGITFRNTAGASN 328

Query: 362 HQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCE 421
            QAVA RS SDLSV   C F   QDTLY HS RQFY+ C + G VDFIFGN+A++ Q+C 
Sbjct: 329 EQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCN 388

Query: 422 ILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFL 481
           I  R    +P + + N ITAQGR+DP Q+TG +  N  +    +   +  S     K +L
Sbjct: 389 IFAR----RP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGST----KTYL 448

Query: 482 GRPWKEYSRTVFIHCNLEALVTPSGWMPWSGDFALKTLFYGEFENSGAGSKTSERVGWSS 541
           GRPW++YSRTVF+  +L++L+ P GW+ W G+FALKTLFY EF+N+G G+ TS RV W  
Sbjct: 449 GRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPG 504

Query: 542 ---QIPAQHVFSYSVQNFIQGDEWIPSS 565
                 A     ++V  F+ G  WIPSS
Sbjct: 509 FRVLGSASEASKFTVGTFLAGGSWIPSS 504

[Close]

BLAST of Lsi10G007180 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 299.7 bits (766), Expect = 5.2e-81
Identity = 189/517 (36.56%), Postives = 281/517 (54.35%), Query Frame = 0


Query: 67  CKATRFPDQCGSSLSRSSLPPNPTPLQILQSAVALSSDGLATAQSMVNQILDSSAKNLNR 126
           C  T + + C +SL ++S P +  PL +++    ++   +  +    +  L + A N   
Sbjct: 59  CAPTDYKETCVNSLMKAS-PDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKD 118

Query: 127 TTAAKNCLEVLRN---SDYRISLTN-DALR----RGRTKDARAWLSASLLYHYDCWSAL- 186
           T  A    E L N    D +  L N D           +D R WLS S+ Y   C     
Sbjct: 119 TKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFE 178

Query: 187 ----KYANDTQLVDKTMAFLDSL-IGLSSNALSMLVAYDISG--NDTAAWTPP-RTERDG 246
               K + D Q + KT   L S  + + +N  ++L  ++++G   D   +     +  DG
Sbjct: 179 ETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSAEDG 238

Query: 247 F--WEGGGGGSGLGFSGGFPSKVAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIH 306
              W G      +   GG    V A+  V  DG+G  Y T+ EA++A P+ A  + FVI+
Sbjct: 239 IPSWVGPNTRRLMATKGG----VKANVVVAHDGSGQ-YKTINEALNAVPK-ANQKPFVIY 298

Query: 307 IKEGVYEEIVRVPLEKKNVVFLGDGMGKTVITCSLNVGQPGISTYNTATVGVLGDGFMAS 366
           IK+GVY E V V  +  +V F+GDG  KT IT SLN     + TY TATV + GD F A 
Sbjct: 299 IKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAK 358

Query: 367 GLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFI 426
            +  +NTAGP+ HQAVA R  +DL+V  +C+  G QDTLY HS RQF++ C + G VDFI
Sbjct: 359 NIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFI 418

Query: 427 FGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSL 486
           FG+   + Q+C I+VR    KP K ++  ITAQGR+D  +STG V QNC I G   Y+ +
Sbjct: 419 FGDGIVVLQNCNIVVR----KPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPV 478

Query: 487 YYSKPKVHKNFLGRPWKEYSRTVFIHCNLEALVTPSGWMPWSGDFALKTLFYGEFENSGA 546
                 ++K +LGRPWKE+SRT+ +   ++ ++ P+GW+PW+GDFAL TL+Y E+EN+G 
Sbjct: 479 ----KSINKAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGP 538

Query: 547 GSKTSERVGWS--SQIPAQHVFSYSVQNFIQGDEWIP 563
           GS  ++RV W    ++  +    ++   F++G+ WIP
Sbjct: 539 GSNQAQRVKWPGIKKLSPKQALRFTPARFLRGNLWIP 560

[Close]

BLAST of Lsi10G007180 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 298.9 bits (764), Expect = 8.9e-81
Identity = 185/522 (35.44%), Postives = 282/522 (54.02%), Query Frame = 0


Query: 67  CKATRFPDQCGSSLSRSSLPPNPTPLQILQSAVALSSDGL-ATAQSMVNQILDSSAKNLN 126
           C  T F D C +SL  +S P +  P+ +++    ++   +  + +     I   + KN  
Sbjct: 55  CAPTDFKDTCVNSLMGAS-PDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPE 114

Query: 127 RTTAAKNCLEVLRNS--------DYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSAL 186
              A + C +++ ++        D+  S+    +     +D R WLS S+ +   C  + 
Sbjct: 115 AKGAFELCEKLMIDAIDDLKKCMDHGFSVDQIEV---FVEDLRVWLSGSIAFQQTCMDSF 174

Query: 187 KYANDTQLVDKTMAFLDSLIGLSSNALSMLVAY-------DISGNDTAAWTPPR---TER 246
                  + D    F  S   LSSN+L+M+          +++G   A     R   +  
Sbjct: 175 GEIKSNLMQDMLKIFKTSR-ELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTE 234

Query: 247 DGF--WEGGGGGSGLGFSGGFPSKVAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFV 306
           D    W G      +   GG P  V A+A V +DG G  + T+ +A++A P+      F+
Sbjct: 235 DSIPTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQ-FKTITDALNAVPKGNKV-PFI 294

Query: 307 IHIKEGVYEEIVRVPLEKKNVVFLGDGMGKTVITCSLNVGQPGISTYNTATVGVLGDGFM 366
           IHIKEG+Y+E V V  +  +V F+GDG  KT+IT SLN G   + T+ TAT+ + GD F 
Sbjct: 295 IHIKEGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFT 354

Query: 367 ASGLTIQNTAGPDAHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVD 426
           A  + I+NTAGP+  QAVA R  +D +V   C+  G+QDTLY HS RQFY+ C + G VD
Sbjct: 355 AKNIGIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVD 414

Query: 427 FIFGNSASIFQDCEILVRPRQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYM 486
           FIFG++  I Q+C+I+VR    KP KG+   +TAQGR++  +STG V   C I G   Y+
Sbjct: 415 FIFGDAKCILQNCKIVVR----KPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYI 474

Query: 487 SLYYSKPKVHKNFLGRPWKEYSRTVFIHCNLEALVTPSGWMPWSGDFALKTLFYGEFENS 546
            +      V+K +LGRPWKE+SRT+ +   ++ ++ P+GW+PWSGDFALKTL+Y E  N+
Sbjct: 475 PM----KSVNKAYLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNT 534

Query: 547 GAGSKTSERVGWS--SQIPAQHVFSYSVQNFIQGDEWIPSSE 566
           G GS  ++RV W    ++  Q    Y+   F++GD WIP ++
Sbjct: 535 GPGSNQAQRVKWPGIKKLTPQDALLYTGDRFLRGDTWIPQTQ 561

The following BLAST results are available for this feature:

BLAST of Lsi10G007180 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LXD9	7.3e-205	66.36	Probable pectinesterase/pectinesterase inhibitor 51 OS=Arabidopsis thaliana OX=3...	[more]
Q8L7Q7	1.0e-182	58.14	Probable pectinesterase/pectinesterase inhibitor 64 OS=Arabidopsis thaliana OX=3...	[more]
O04887	7.1e-83	38.99	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
O22149	4.3e-80	38.19	Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3...	[more]
Q9FJ21	7.3e-80	36.56	Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Lsi10G007180 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CD05	2.2e-310	92.54	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499643 PE=3 SV=1	[more]
A0A0A0LQ80	1.8e-307	91.67	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G302130 PE=3 SV=1	[more]
A0A5A7TBR9	3.1e-291	95.39	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold680G00730...	[more]
A0A6J1EV58	3.0e-286	85.52	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111438335 PE=3 SV=1	[more]
A0A6J1I3S8	2.6e-282	85.00	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470304 PE=3 SV=1	[more]

BLAST of Lsi10G007180 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038902126.1	0.0e+00	95.20	probable pectinesterase/pectinesterase inhibitor 51 [Benincasa hispida]	[more]
XP_008460902.1	4.5e-310	92.54	PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor...	[more]
XP_004147375.2	3.7e-307	91.67	probable pectinesterase/pectinesterase inhibitor 51 [Cucumis sativus] >KGN62146....	[more]
KAA0040770.1	6.4e-291	95.39	putative pectinesterase/pectinesterase inhibitor 51 [Cucumis melo var. makuwa] >...	[more]
XP_022932006.1	6.2e-286	85.52	probable pectinesterase/pectinesterase inhibitor 51 [Cucurbita moschata]	[more]

BLAST of Lsi10G007180 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G09760.1	5.2e-206	66.36	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G64640.1	7.3e-184	58.14	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G45220.1	3.1e-81	38.19	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G49180.1	5.2e-81	36.56	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G06830.1	8.9e-81	35.44	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 58..206 e-value: 1.1E-16 score: 71.4
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 64..206 e-value: 1.3E-16 score: 61.3
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 31..206 e-value: 5.9E-12 score: 43.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 59..209 e-value: 2.3E-18 score: 68.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 61..206
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 253..558 e-value: 2.4E-122 score: 407.3
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 247..571 e-value: 4.8E-111 score: 373.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 562..583
None	No IPR available	PANTHER	PTHR31707:SF340	PECTINESTERASE	coord: 19..564
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 19..564
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 66..206 e-value: 1.34297E-19 score: 84.0269
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 250..563

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G007180.1	Lsi10G007180.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity