Lsi10G006950 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G006950
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
Locationchr10: 9747201 .. 9748217 (+)
RNA-Seq ExpressionLsi10G006950
SyntenyLsi10G006950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGGCGTCCAGTTCTTATTTGGAAATCTCAACCTCCCAACTTCCATGGAAGCACAAACTTACTCTTCGAGTCGCTGCTCTACTCTTCAACACTTTTCTCCGATCAAATCTCACCGTCAATCGTTGGCTTCTCAATTTTTTCGACCCCAAAATCCCTCCCAATCCCAATCTCAACCACGCCGTTTCCTCTTCCGATCTCACCATCGATGCTTCTCGAGATCTCTTCCTTCGCATCTTCACCCCCACCGCTGCCGCTGCCGCCGCCGACTCACTCCCGGCACCGGTTATTTTCTACTTCCACGGCGGCGGCTTCGCCATCGGTTCCGCCGACTCCACTTCAATGGACTTGGCCGCCACTCGCTTCGCTAAAGAACTACGCGCCGTTGTAATCTCCGTCAATTACCGCCTCGCTCCTGAGTTCCGATTCCCCTGCCAATACGACGACGGATTCGATGCTTTGAAATTCATCGACGAAATGGACGACAGCGCATTGATGGGAAGGGCAGATCTAAGCCATTGCTTCATTTTGGGGGAAAGCGCCGGGGGGAATTTGGGCCACCACGTGGCGGTGAGGGCAAGCGAGTACGCGTTCAAGAGAGTGAAGGTGGTTGGATTTATAGCGTCGCAGCCGTTTTTCGGTGGAGAGGAGAGAACAGAGTCGGAGATTCGGCTCTGTAATGAGCCTCTGTTGACTCTGAAAATTACTGATTGGTTTTGGAAGGCGTTTTTGCCGGCCGGGGAAAACCGTGACCACGCGGCGGTGAATGTGAATGGACCCAATGGAAGGGATATATTGGGGGTGAGGAACTTTCCGGCGACGGTGATTTTCGCCGGAGAATTGGATCTTCTGATTGATCGGCATAAGAGTTATTACGAGTGGTTAATGAAAATGGAAAAGGATGTGAAGTTGGTGGTGTTCTCGAACGCCGTCCATGGATTCTCTGTCTTTGCGGATCTGCCGGAATATTCTTTGATGGTTAAAGAGATAAAGGATTTCATTGCAAAGCTGAGATAG

mRNA sequence

ATGTGGGCGTCCAGTTCTTATTTGGAAATCTCAACCTCCCAACTTCCATGGAAGCACAAACTTACTCTTCGAGTCGCTGCTCTACTCTTCAACACTTTTCTCCGATCAAATCTCACCGTCAATCGTTGGCTTCTCAATTTTTTCGACCCCAAAATCCCTCCCAATCCCAATCTCAACCACGCCGTTTCCTCTTCCGATCTCACCATCGATGCTTCTCGAGATCTCTTCCTTCGCATCTTCACCCCCACCGCTGCCGCTGCCGCCGCCGACTCACTCCCGGCACCGGTTATTTTCTACTTCCACGGCGGCGGCTTCGCCATCGGTTCCGCCGACTCCACTTCAATGGACTTGGCCGCCACTCGCTTCGCTAAAGAACTACGCGCCGTTGTAATCTCCGTCAATTACCGCCTCGCTCCTGAGTTCCGATTCCCCTGCCAATACGACGACGGATTCGATGCTTTGAAATTCATCGACGAAATGGACGACAGCGCATTGATGGGAAGGGCAGATCTAAGCCATTGCTTCATTTTGGGGGAAAGCGCCGGGGGGAATTTGGGCCACCACGTGGCGGTGAGGGCAAGCGAGTACGCGTTCAAGAGAGTGAAGGTGGTTGGATTTATAGCGTCGCAGCCGTTTTTCGGTGGAGAGGAGAGAACAGAGTCGGAGATTCGGCTCTGTAATGAGCCTCTGTTGACTCTGAAAATTACTGATTGGTTTTGGAAGGCGTTTTTGCCGGCCGGGGAAAACCGTGACCACGCGGCGGTGAATGTGAATGGACCCAATGGAAGGGATATATTGGGGGTGAGGAACTTTCCGGCGACGGTGATTTTCGCCGGAGAATTGGATCTTCTGATTGATCGGCATAAGAGTTATTACGAGTGGTTAATGAAAATGGAAAAGGATGTGAAGTTGGTGGTGTTCTCGAACGCCGTCCATGGATTCTCTGTCTTTGCGGATCTGCCGGAATATTCTTTGATGGTTAAAGAGATAAAGGATTTCATTGCAAAGCTGAGATAG

Coding sequence (CDS)

ATGTGGGCGTCCAGTTCTTATTTGGAAATCTCAACCTCCCAACTTCCATGGAAGCACAAACTTACTCTTCGAGTCGCTGCTCTACTCTTCAACACTTTTCTCCGATCAAATCTCACCGTCAATCGTTGGCTTCTCAATTTTTTCGACCCCAAAATCCCTCCCAATCCCAATCTCAACCACGCCGTTTCCTCTTCCGATCTCACCATCGATGCTTCTCGAGATCTCTTCCTTCGCATCTTCACCCCCACCGCTGCCGCTGCCGCCGCCGACTCACTCCCGGCACCGGTTATTTTCTACTTCCACGGCGGCGGCTTCGCCATCGGTTCCGCCGACTCCACTTCAATGGACTTGGCCGCCACTCGCTTCGCTAAAGAACTACGCGCCGTTGTAATCTCCGTCAATTACCGCCTCGCTCCTGAGTTCCGATTCCCCTGCCAATACGACGACGGATTCGATGCTTTGAAATTCATCGACGAAATGGACGACAGCGCATTGATGGGAAGGGCAGATCTAAGCCATTGCTTCATTTTGGGGGAAAGCGCCGGGGGGAATTTGGGCCACCACGTGGCGGTGAGGGCAAGCGAGTACGCGTTCAAGAGAGTGAAGGTGGTTGGATTTATAGCGTCGCAGCCGTTTTTCGGTGGAGAGGAGAGAACAGAGTCGGAGATTCGGCTCTGTAATGAGCCTCTGTTGACTCTGAAAATTACTGATTGGTTTTGGAAGGCGTTTTTGCCGGCCGGGGAAAACCGTGACCACGCGGCGGTGAATGTGAATGGACCCAATGGAAGGGATATATTGGGGGTGAGGAACTTTCCGGCGACGGTGATTTTCGCCGGAGAATTGGATCTTCTGATTGATCGGCATAAGAGTTATTACGAGTGGTTAATGAAAATGGAAAAGGATGTGAAGTTGGTGGTGTTCTCGAACGCCGTCCATGGATTCTCTGTCTTTGCGGATCTGCCGGAATATTCTTTGATGGTTAAAGAGATAAAGGATTTCATTGCAAAGCTGAGATAG

Protein sequence

MWASSSYLEISTSQLPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRDLFLRIFTPTAAAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGESAGGNLGHHVAVRASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRDHAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAVHGFSVFADLPEYSLMVKEIKDFIAKLR
Homology
BLAST of Lsi10G006950 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 2.6e-77
Identity = 153/323 (47.37%), Postives = 203/323 (62.85%), Query Frame = 0

Query: 15  LPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRD 74
           LP K ++ L V + + +   R + T+NR  L  FD + PPNP   + VS+SD  +D SRD
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 75  LFLRIFTPTAAAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISVN 134
           L+ R++TP     + D +  PV+ +FHGGGFA  S ++   D    RFA++L A VISVN
Sbjct: 73  LWFRLYTP---HVSGDKI--PVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVN 132

Query: 135 YRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGESAGGNLGHHVAV--- 194
           YRLAPE R+P QYDDGFDALK+I+E   S L   ADLS CF  G+SAGGN+ H+VA+   
Sbjct: 133 YRLAPEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRIC 192

Query: 195 RASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRD 254
           R    +F  VK++G I+ QPFFGGEERTE+E +L   PL++   TDW WKA    G NRD
Sbjct: 193 REPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNRD 252

Query: 255 HAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAV 314
           H AVNV GPN  DI G+ ++P T++     D L D  +SYYEWL    K   L+ + N  
Sbjct: 253 HEAVNVGGPNAVDISGL-DYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNMF 312

Query: 315 HGFSVFADLPEYSLMVKEIKDFI 335
           H F +F +LPE   ++  IKDF+
Sbjct: 313 HAFYIFPELPEAGQLIMRIKDFV 326

BLAST of Lsi10G006950 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 6.8e-54
Identity = 124/317 (39.12%), Postives = 175/317 (55.21%), Query Frame = 0

Query: 28  LLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRDLFLRIFTPTAAAA 87
           L +N   R + T NR L  F D K+P N N  + V S D+ ID   +L  R++ P  A  
Sbjct: 29  LAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGT 88

Query: 88  A---------ADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISVNYRLA 147
           +          D    PVI +FHGG FA  SA+S   D    R      AVV+SVNYR A
Sbjct: 89  SPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRA 148

Query: 148 PEFRFPCQYDDGFDALKFIDEMDDSALMGRADLS-HCFILGESAGGNLGHHVAVRASEYA 207
           PE R+PC YDDG+  LK+++    S L  + D     F+ G+S+GGN+ H+VAVRA E  
Sbjct: 149 PENRYPCAYDDGWAVLKWVN--SSSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE-- 208

Query: 208 FKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRDHAAVNV 267
             R+ V+G I   P FGG ERTESE RL  +  +T++  DW+W+AFLP GE+R+H A + 
Sbjct: 209 -SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSP 268

Query: 268 NGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAVHGFSVF 327
            GP  + + G+ +FP +++    LDL+ D    Y E L K  ++VKL+    A  GF + 
Sbjct: 269 FGPRSKSLEGL-SFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYLL 328

Query: 328 ADLPEYSLMVKEIKDFI 335
            +   +  ++ EI  F+
Sbjct: 329 PNNNHFHTVMDEIAAFV 339

BLAST of Lsi10G006950 vs. ExPASy Swiss-Prot
Match: Q6L545 (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.2e-53
Identity = 131/325 (40.31%), Postives = 173/325 (53.23%), Query Frame = 0

Query: 28  LLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRDLFLRIFTPTAAAA 87
           L +N   R++ T  R L  + D ++P N      VSS D  ID S  L +RI+   A   
Sbjct: 29  LSYNILRRADGTFERDLGEYLDRRVPANARPLEGVSSFDHIIDQSVGLEVRIYRAAAEGD 88

Query: 88  AADSL------------------PAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAV 147
           A +                    P PVI +FHGG F   SA ST  D    RF K  + V
Sbjct: 89  AEEGAAAVTRPILEFLTDAPAAEPFPVIIFFHGGSFVHSSASSTIYDSLCRRFVKLSKGV 148

Query: 148 VISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGESAGGNLGHHV 207
           V+SVNYR APE R+PC YDDG+ ALK++         G A  +  F+ G+S+GGN+ HHV
Sbjct: 149 VVSVNYRRAPEHRYPCAYDDGWTALKWVMSQPFMRSGGDAQ-ARVFLSGDSSGGNIAHHV 208

Query: 208 AVRASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGEN 267
           AVRA++   + VKV G I     FGG ERTESE RL  +  +TL+  DW+WKA+LP   +
Sbjct: 209 AVRAAD---EGVKVCGNILLNAMFGGTERTESERRLDGKYFVTLQDRDWYWKAYLPEDAD 268

Query: 268 RDHAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSN 327
           RDH A N  GPNGR  LG   F  ++I    LDL  DR  +Y + L +    VK+V   N
Sbjct: 269 RDHPACNPFGPNGRR-LGGLPFAKSLIIVSGLDLTCDRQLAYADALREDGHHVKVVQCEN 328

Query: 328 AVHGFSVFADLPEYSLMVKEIKDFI 335
           A  GF +  +   Y  +++EI DF+
Sbjct: 329 ATVGFYLLPNTVHYHEVMEEISDFL 348

BLAST of Lsi10G006950 vs. ExPASy Swiss-Prot
Match: Q9MAA7 (Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 7.1e-51
Identity = 120/317 (37.85%), Postives = 170/317 (53.63%), Query Frame = 0

Query: 30  FNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRDLFLRIFTPTAA---- 89
           +N   R + T NR L  + D K+  N N    V S D+ ID   +L  R++ P  A    
Sbjct: 31  YNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQ 90

Query: 90  -------AAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISVNYRLA 149
                      D    PVI +FHGG FA  SA+S   D    R     + VV+SVNYR A
Sbjct: 91  PPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRA 150

Query: 150 PEFRFPCQYDDGFDALKFIDEMDDSALMGRADLS-HCFILGESAGGNLGHHVAVRASEYA 209
           PE  +PC YDDG+ AL +++    S L  + D   H F+ G+S+GGN+ H+VA+RA E  
Sbjct: 151 PENPYPCAYDDGWIALNWVNSR--SWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGE-- 210

Query: 210 FKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRDHAAVNV 269
              + V+G I   P FGG ERTESE  L  +  +T++  DW+WKAFLP GE+R+H A N 
Sbjct: 211 -SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACNP 270

Query: 270 NGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAVHGFSVF 329
             P G+ + GV +FP +++    LDL+ D   +Y E L K  ++VKL+    A  GF + 
Sbjct: 271 FSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLL 330

Query: 330 ADLPEYSLMVKEIKDFI 335
            +   +  ++ EI  F+
Sbjct: 331 PNNNHFHNVMDEISAFV 341

BLAST of Lsi10G006950 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.7e-47
Identity = 110/319 (34.48%), Postives = 172/319 (53.92%), Query Frame = 0

Query: 28  LLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRDLFLRIFTPTA--- 87
           L +    R + + NR L  F D K+P N      V S D  +D++ +L  RI+ P +   
Sbjct: 29  LAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTNLLTRIYQPASLLH 88

Query: 88  ---------AAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISVNY 147
                        + +   PV+ +FHGG F   SA+S   D    R       VV+SV+Y
Sbjct: 89  QTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDY 148

Query: 148 RLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGESAGGNLGHHVAVRASE 207
           R +PE R+PC YDDG++AL ++ +       G+    + ++ G+S+GGN+ H+VAVRA+ 
Sbjct: 149 RRSPEHRYPCAYDDGWNALNWV-KSRVWLQSGKDSNVYVYLAGDSSGGNIAHNVAVRATN 208

Query: 208 YAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRDHAAV 267
              + VKV+G I   P FGG+ERT+SE  L  +  +T++  DW+W+A+LP GE+RDH A 
Sbjct: 209 ---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDRDHPAC 268

Query: 268 NVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAVHGFS 327
           N  GP G+ + GV NFP +++    LDL+ D   +Y + L K   +V L+    A  GF 
Sbjct: 269 NPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQATIGFY 328

Query: 328 VFADLPEYSLMVKEIKDFI 335
              +   +  +++E+  F+
Sbjct: 329 FLPNNDHFHCLMEELNKFV 341

BLAST of Lsi10G006950 vs. ExPASy TrEMBL
Match: A0A6J1IER7 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111472040 PE=3 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.4e-150
Identity = 267/327 (81.65%), Postives = 290/327 (88.69%), Query Frame = 0

Query: 9   EISTSQLPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLT 68
           EISTSQLPWKH+LTLR AALLFN  +RS+LTVNR LLNF DPKIPPNPN  HAVSSSDLT
Sbjct: 9   EISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLT 68

Query: 69  IDASRDLFLRIFTPTAAAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRA 128
           IDASRDLFLRIFTPT   AA DSL  PVIFYFHGGGFA GSA +TSMD+AA RF+KEL A
Sbjct: 69  IDASRDLFLRIFTPT--VAATDSLLPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGA 128

Query: 129 VVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDD-SALMGRADLSHCFILGESAGGNLGH 188
           VVISVNYRL+P+FRFPCQYDDGFDA+KFIDEMDD   L+GRADLS CFILGESAGGNLGH
Sbjct: 129 VVISVNYRLSPQFRFPCQYDDGFDAVKFIDEMDDEDPLLGRADLSRCFILGESAGGNLGH 188

Query: 189 HVAVRASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAG 248
           HVAVRASEYAFKRVKV+GFIASQPFFGGEERTESEIRLCN+P LTLK+ DWFWKAFLPAG
Sbjct: 189 HVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAG 248

Query: 249 ENRDHAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVF 308
           E+RDHAA NVNGPNGRD+ G+RNFPAT+I AG LD LIDR + YYE L +M KDVKLVVF
Sbjct: 249 EDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDFLIDRQRRYYEELKRMGKDVKLVVF 308

Query: 309 SNAVHGFSVFADLPEYSLMVKEIKDFI 335
           +N VHGF  F DLPEYSLM+KE++DFI
Sbjct: 309 TNGVHGFFSFEDLPEYSLMIKEMRDFI 333

BLAST of Lsi10G006950 vs. ExPASy TrEMBL
Match: A0A6J1ECC8 (probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111432777 PE=3 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 3.1e-150
Identity = 269/336 (80.06%), Postives = 294/336 (87.50%), Query Frame = 0

Query: 1   MWASSSYLEISTSQLPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNH 60
           M  SS   EISTSQLPWKH+LTLR AALLFN  +RS+LTVNR LLNF DPKIPPNPN  H
Sbjct: 1   MQPSSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  AVSSSDLTIDASRDLFLRIFTPTAAAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAAT 120
           AVSSSDLTID SRDLFLRIFTPT   AA DS+  PVIFYFHGGGFA GSAD+TSMD+AA 
Sbjct: 61  AVSSSDLTIDDSRDLFLRIFTPT--VAATDSVLPPVIFYFHGGGFAFGSADATSMDMAAR 120

Query: 121 RFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEM-DDSALMGRADLSHCFILGE 180
           RF+KEL AVVISVNYRLAP+FRFPCQYDDGFDA+KFIDEM DD  L+G+ADLS CFILGE
Sbjct: 121 RFSKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWF 240
           SAGGNLGHHVAVRASEYAFKRVKV+GFIASQPFFGGEERTESEIRLCN+P LTLK+ DWF
Sbjct: 181 SAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWF 240

Query: 241 WKAFLPAGENRDHAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKME 300
           WKAFLPAGE+RDHAA NVNGPNGRD+ G+RNFPAT+I AG LDLLIDR + YYE L +M 
Sbjct: 241 WKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRMG 300

Query: 301 KDVKLVVFSNAVHGFSVFADLPEYSLMVKEIKDFIA 336
           K+VKLVVF+N VHGF  F D PEYSLM+KE++DFIA
Sbjct: 301 KEVKLVVFTNGVHGFFSFEDPPEYSLMIKEMRDFIA 334

BLAST of Lsi10G006950 vs. ExPASy TrEMBL
Match: A0A0A0KXS8 (Acetyl esterase OS=Cucumis sativus OX=3659 GN=Csa_5G647390 PE=3 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 7.1e-147
Identity = 263/338 (77.81%), Postives = 289/338 (85.50%), Query Frame = 0

Query: 1   MWASSSYLEISTSQLPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNH 60
           M +SS   E+ST QLPWKHK+TLR A LLFNT LRS+ TVNR LL F DPKIPPNPN  H
Sbjct: 1   MRSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60

Query: 61  AVSSSDLTIDASRDLFLRIFTPTAAAAAADSLP-APVIFYFHGGGFAIGSADSTSMDLAA 120
           +VSSSDLTID SRDLFLRIFTP   AA  +SLP  P+IFYFHGGGFA GSAD+TS D+AA
Sbjct: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120

Query: 121 TRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGE 180
             FA++LRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDD +L+ R DLS CFILGE
Sbjct: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWF 240
           SAGGNLGHHVAVRASEY FKRVK++GFIASQPFFGG+ERTESE RLC +  LTL +TDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240

Query: 241 WKAFLPAGENRDHAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKME 300
           W+AFLPAGE+RDHAA NVNGPNGRDI G+ NFPATVIFAG LDLL+DR KSYYE L +M 
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300

Query: 301 KDVKLVVFSNAVHGFSVFADLPEYSLMVKEIKDFIAKL 338
           KDVKLVVFSNA HGF  F DLPEYSLM++E+ DFIAKL
Sbjct: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKL 338

BLAST of Lsi10G006950 vs. ExPASy TrEMBL
Match: A0A1S3CBA4 (probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498755 PE=3 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 1.8e-145
Identity = 259/337 (76.85%), Postives = 289/337 (85.76%), Query Frame = 0

Query: 1   MWASSSYLEISTSQLPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNH 60
           M +S    +IST QLPWKH++TLR A LL+NT LRS+LTVNR LLNF DPKIP N +  H
Sbjct: 1   MCSSGVCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTH 60

Query: 61  AVSSSDLTIDASRDLFLRIFTPTAAAAAADSLP-APVIFYFHGGGFAIGSADSTSMDLAA 120
           +VSSSDL +D SRDLFLRIFTPT  AA  +SLP  P+IFYFHGGGF  GSAD+TS D+AA
Sbjct: 61  SVSSSDLIVDTSRDLFLRIFTPTPTAALDESLPLLPIIFYFHGGGFVFGSADATSTDMAA 120

Query: 121 TRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGE 180
            RFAK+LRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDD +L+ R DLS CFILGE
Sbjct: 121 RRFAKKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSCCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWF 240
           SAGGNLGHHVAVRASEY FKRVK++GFIASQPFFGG+ERTESE RLC +  LTL +TDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMTDWF 240

Query: 241 WKAFLPAGENRDHAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKME 300
           W+AFLPAGE+RDHAA NVNGPNGRDI G+ NFPAT+IFAG LDLLIDR K YYE L +M 
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLKRMG 300

Query: 301 KDVKLVVFSNAVHGFSVFADLPEYSLMVKEIKDFIAK 337
           KDVKLVVFSNAVHG+  FADLPEYSLM+KE++DFIAK
Sbjct: 301 KDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIAK 337

BLAST of Lsi10G006950 vs. ExPASy TrEMBL
Match: A0A5D3BL91 (Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001330 PE=3 SV=1)

HSP 1 Score: 516.5 bits (1329), Expect = 8.2e-143
Identity = 255/337 (75.67%), Postives = 285/337 (84.57%), Query Frame = 0

Query: 1   MWASSSYLEISTSQLPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNH 60
           M +S    +IST QLPWKH++TLR A LL+NT LRS+LTVNR LLNF DPKIP N +  H
Sbjct: 1   MCSSGVCYQISTPQLPWKHRITLRFATLLYNTSLRSDLTVNRRLLNFIDPKIPSNHDSTH 60

Query: 61  AVSSSDLTIDASRDLFLRIFTPTAAAAAADSLP-APVIFYFHGGGFAIGSADSTSMDLAA 120
           +VSSSDL +D SRDLFLRIFTPT  AA  +SLP  P+IFYFHGGGF  GSAD+TS D+AA
Sbjct: 61  SVSSSDLIVDTSRDLFLRIFTPTPTAALDESLPLLPIIFYFHGGGFVFGSADATSTDMAA 120

Query: 121 TRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGE 180
            RFAK+L AVVISVNYRLAPEFRFPCQ DDGFD LKFIDEMDD +L+ R DLS CFILGE
Sbjct: 121 RRFAKKLCAVVISVNYRLAPEFRFPCQCDDGFDPLKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWF 240
           SAGGNLGHHVAVRASEY FKRVK++GFIASQPFFGG+ERTESE RLC +  LTL +TDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLDMTDWF 240

Query: 241 WKAFLPAGENRDHAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKME 300
           W+AFLPAGE+RDHA  NVNGPNGRDI G+ NFPAT+IFAG LDLLIDR K YYE L +M 
Sbjct: 241 WRAFLPAGEDRDHATANVNGPNGRDISGLENFPATLIFAGGLDLLIDRQKCYYERLKRMG 300

Query: 301 KDVKLVVFSNAVHGFSVFADLPEYSLMVKEIKDFIAK 337
           KDVKLVVFSNAVHG+  FADLPEYSLM+KE++DFIAK
Sbjct: 301 KDVKLVVFSNAVHGYFGFADLPEYSLMIKEMRDFIAK 337

BLAST of Lsi10G006950 vs. NCBI nr
Match: XP_023536262.1 (probable carboxylesterase 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 543.5 bits (1399), Expect = 1.3e-150
Identity = 269/334 (80.54%), Postives = 294/334 (88.02%), Query Frame = 0

Query: 9   EISTSQLPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLT 68
           EISTSQLPWKH+LTLR AALLFN  +RS+LTVNR LLNF DPKIPPNPN  HAVSSSDLT
Sbjct: 9   EISTSQLPWKHRLTLRFAALLFNASVRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLT 68

Query: 69  IDASRDLFLRIFTPT------AAAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRF 128
           IDASRDLFLRIFTPT      AA AA DSL  PVIFYFHGGGFA GSA +TSMD+AA RF
Sbjct: 69  IDASRDLFLRIFTPTDAAINAAAVAATDSLLPPVIFYFHGGGFAFGSAAATSMDMAARRF 128

Query: 129 AKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEM-DDSALMGRADLSHCFILGESA 188
           +KEL AV+ISVNYRLAP+FRFPCQYDDGFDA+KFIDEM DD  L+G+ADLS CFILGESA
Sbjct: 129 SKELGAVIISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESA 188

Query: 189 GGNLGHHVAVRASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWK 248
           GGNLGHHVAVRASEYAFKRVKV+GFIASQPFFGGEERTESEIRLCN+P LTLK+ DWFWK
Sbjct: 189 GGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWK 248

Query: 249 AFLPAGENRDHAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKD 308
           AFLPAGE+RDHAA NVNGPNGRD+ G+RNFPAT+I AG LDLLIDR + YYE L +M K+
Sbjct: 249 AFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRMGKE 308

Query: 309 VKLVVFSNAVHGFSVFADLPEYSLMVKEIKDFIA 336
           VKLVVF+N VHGF  F DLPEYSLM+KE++DFIA
Sbjct: 309 VKLVVFTNGVHGFFSFEDLPEYSLMIKEMRDFIA 342

BLAST of Lsi10G006950 vs. NCBI nr
Match: XP_022973499.1 (probable carboxylesterase 18 [Cucurbita maxima])

HSP 1 Score: 541.6 bits (1394), Expect = 4.9e-150
Identity = 267/327 (81.65%), Postives = 290/327 (88.69%), Query Frame = 0

Query: 9   EISTSQLPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLT 68
           EISTSQLPWKH+LTLR AALLFN  +RS+LTVNR LLNF DPKIPPNPN  HAVSSSDLT
Sbjct: 9   EISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLT 68

Query: 69  IDASRDLFLRIFTPTAAAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRA 128
           IDASRDLFLRIFTPT   AA DSL  PVIFYFHGGGFA GSA +TSMD+AA RF+KEL A
Sbjct: 69  IDASRDLFLRIFTPT--VAATDSLLPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGA 128

Query: 129 VVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDD-SALMGRADLSHCFILGESAGGNLGH 188
           VVISVNYRL+P+FRFPCQYDDGFDA+KFIDEMDD   L+GRADLS CFILGESAGGNLGH
Sbjct: 129 VVISVNYRLSPQFRFPCQYDDGFDAVKFIDEMDDEDPLLGRADLSRCFILGESAGGNLGH 188

Query: 189 HVAVRASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAG 248
           HVAVRASEYAFKRVKV+GFIASQPFFGGEERTESEIRLCN+P LTLK+ DWFWKAFLPAG
Sbjct: 189 HVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAG 248

Query: 249 ENRDHAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVF 308
           E+RDHAA NVNGPNGRD+ G+RNFPAT+I AG LD LIDR + YYE L +M KDVKLVVF
Sbjct: 249 EDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDFLIDRQRRYYEELKRMGKDVKLVVF 308

Query: 309 SNAVHGFSVFADLPEYSLMVKEIKDFI 335
           +N VHGF  F DLPEYSLM+KE++DFI
Sbjct: 309 TNGVHGFFSFEDLPEYSLMIKEMRDFI 333

BLAST of Lsi10G006950 vs. NCBI nr
Match: XP_022925491.1 (probable carboxylesterase 18 [Cucurbita moschata])

HSP 1 Score: 541.2 bits (1393), Expect = 6.4e-150
Identity = 269/336 (80.06%), Postives = 294/336 (87.50%), Query Frame = 0

Query: 1   MWASSSYLEISTSQLPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNH 60
           M  SS   EISTSQLPWKH+LTLR AALLFN  +RS+LTVNR LLNF DPKIPPNPN  H
Sbjct: 1   MQPSSVSSEISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIH 60

Query: 61  AVSSSDLTIDASRDLFLRIFTPTAAAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAAT 120
           AVSSSDLTID SRDLFLRIFTPT   AA DS+  PVIFYFHGGGFA GSAD+TSMD+AA 
Sbjct: 61  AVSSSDLTIDDSRDLFLRIFTPT--VAATDSVLPPVIFYFHGGGFAFGSADATSMDMAAR 120

Query: 121 RFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEM-DDSALMGRADLSHCFILGE 180
           RF+KEL AVVISVNYRLAP+FRFPCQYDDGFDA+KFIDEM DD  L+G+ADLS CFILGE
Sbjct: 121 RFSKELGAVVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWF 240
           SAGGNLGHHVAVRASEYAFKRVKV+GFIASQPFFGGEERTESEIRLCN+P LTLK+ DWF
Sbjct: 181 SAGGNLGHHVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWF 240

Query: 241 WKAFLPAGENRDHAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKME 300
           WKAFLPAGE+RDHAA NVNGPNGRD+ G+RNFPAT+I AG LDLLIDR + YYE L +M 
Sbjct: 241 WKAFLPAGEDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDLLIDRQRRYYEELKRMG 300

Query: 301 KDVKLVVFSNAVHGFSVFADLPEYSLMVKEIKDFIA 336
           K+VKLVVF+N VHGF  F D PEYSLM+KE++DFIA
Sbjct: 301 KEVKLVVFTNGVHGFFSFEDPPEYSLMIKEMRDFIA 334

BLAST of Lsi10G006950 vs. NCBI nr
Match: KAG6592331.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025153.1 putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 538.9 bits (1387), Expect = 3.2e-149
Identity = 265/328 (80.79%), Postives = 291/328 (88.72%), Query Frame = 0

Query: 9   EISTSQLPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLT 68
           EISTSQLPWKH+LTLR AALLFN  +RS+LTVNR LLNF DPKIPPNPN  HAVSSSDLT
Sbjct: 9   EISTSQLPWKHRLTLRFAALLFNASIRSDLTVNRRLLNFLDPKIPPNPNSIHAVSSSDLT 68

Query: 69  IDASRDLFLRIFTPTAAAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRA 128
           ID SRDLFLRIFTPT   AA DS+  PVIFYFHGGGFA GSA +TSMD+AA RF+KEL A
Sbjct: 69  IDDSRDLFLRIFTPT--VAATDSVLPPVIFYFHGGGFAFGSAAATSMDMAARRFSKELGA 128

Query: 129 VVISVNYRLAPEFRFPCQYDDGFDALKFIDEM-DDSALMGRADLSHCFILGESAGGNLGH 188
           VVISVNYRLAP+FRFPCQYDDGFDA+KFIDEM DD  L+G+ADLS CFILGESAGGNLGH
Sbjct: 129 VVISVNYRLAPQFRFPCQYDDGFDAVKFIDEMDDDDPLLGKADLSRCFILGESAGGNLGH 188

Query: 189 HVAVRASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAG 248
           HVAVRASEYAFKRVKV+GFIASQPFFGGEERTESEIRLCN+P LTLK+ DWFWKAFLPAG
Sbjct: 189 HVAVRASEYAFKRVKVIGFIASQPFFGGEERTESEIRLCNQPPLTLKLADWFWKAFLPAG 248

Query: 249 ENRDHAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVF 308
           E+RDHAA NVNGPNGRD+ G+RNFPAT+I AG LD+LIDR + YYE L +M K+VKLVVF
Sbjct: 249 EDRDHAAANVNGPNGRDVSGLRNFPATIILAGGLDILIDRQRRYYEELKRMGKEVKLVVF 308

Query: 309 SNAVHGFSVFADLPEYSLMVKEIKDFIA 336
           +N VHGF  F DLPEYSLM+KE++DFIA
Sbjct: 309 TNGVHGFFSFEDLPEYSLMIKEMRDFIA 334

BLAST of Lsi10G006950 vs. NCBI nr
Match: XP_004141618.1 (probable carboxylesterase 18 [Cucumis sativus] >KGN52616.1 hypothetical protein Csa_008680 [Cucumis sativus])

HSP 1 Score: 530.0 bits (1364), Expect = 1.5e-146
Identity = 263/338 (77.81%), Postives = 289/338 (85.50%), Query Frame = 0

Query: 1   MWASSSYLEISTSQLPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNH 60
           M +SS   E+ST QLPWKHK+TLR A LLFNT LRS+ TVNR LL F DPKIPPNPN  H
Sbjct: 1   MRSSSVCYELSTPQLPWKHKVTLRFATLLFNTSLRSDFTVNRRLLTFLDPKIPPNPNSAH 60

Query: 61  AVSSSDLTIDASRDLFLRIFTPTAAAAAADSLP-APVIFYFHGGGFAIGSADSTSMDLAA 120
           +VSSSDLTID SRDLFLRIFTP   AA  +SLP  P+IFYFHGGGFA GSAD+TS D+AA
Sbjct: 61  SVSSSDLTIDTSRDLFLRIFTPNPTAALDESLPLLPIIFYFHGGGFAFGSADATSTDMAA 120

Query: 121 TRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGE 180
             FA++LRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDD +L+ R DLS CFILGE
Sbjct: 121 RGFAEKLRAVVISVNYRLAPEFRFPCQYDDGFDALKFIDEMDDDSLLERVDLSRCFILGE 180

Query: 181 SAGGNLGHHVAVRASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWF 240
           SAGGNLGHHVAVRASEY FKRVK++GFIASQPFFGG+ERTESE RLC +  LTL +TDWF
Sbjct: 181 SAGGNLGHHVAVRASEYEFKRVKIIGFIASQPFFGGKERTESENRLCKQLPLTLYMTDWF 240

Query: 241 WKAFLPAGENRDHAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKME 300
           W+AFLPAGE+RDHAA NVNGPNGRDI G+ NFPATVIFAG LDLL+DR KSYYE L +M 
Sbjct: 241 WRAFLPAGEDRDHAAANVNGPNGRDISGLENFPATVIFAGGLDLLMDRQKSYYERLKRMG 300

Query: 301 KDVKLVVFSNAVHGFSVFADLPEYSLMVKEIKDFIAKL 338
           KDVKLVVFSNA HGF  F DLPEYSLM++E+ DFIAKL
Sbjct: 301 KDVKLVVFSNAFHGFFGFPDLPEYSLMIEEMSDFIAKL 338

BLAST of Lsi10G006950 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 290.4 bits (742), Expect = 1.8e-78
Identity = 153/323 (47.37%), Postives = 203/323 (62.85%), Query Frame = 0

Query: 15  LPWKHKLTLRVAALLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRD 74
           LP K ++ L V + + +   R + T+NR  L  FD + PPNP   + VS+SD  +D SRD
Sbjct: 13  LPLKTRIALTVISTMTDNAQRPDGTINRRFLRLFDFRAPPNPKPVNIVSTSDFVVDQSRD 72

Query: 75  LFLRIFTPTAAAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISVN 134
           L+ R++TP     + D +  PV+ +FHGGGFA  S ++   D    RFA++L A VISVN
Sbjct: 73  LWFRLYTP---HVSGDKI--PVVVFFHGGGFAFLSPNAYPYDNVCRRFARKLPAYVISVN 132

Query: 135 YRLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGESAGGNLGHHVAV--- 194
           YRLAPE R+P QYDDGFDALK+I+E   S L   ADLS CF  G+SAGGN+ H+VA+   
Sbjct: 133 YRLAPEHRYPAQYDDGFDALKYIEENHGSILPANADLSRCFFAGDSAGGNIAHNVAIRIC 192

Query: 195 RASEYAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRD 254
           R    +F  VK++G I+ QPFFGGEERTE+E +L   PL++   TDW WKA    G NRD
Sbjct: 193 REPRSSFTAVKLIGLISIQPFFGGEERTEAEKQLVGAPLVSPDRTDWCWKAM---GLNRD 252

Query: 255 HAAVNVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAV 314
           H AVNV GPN  DI G+ ++P T++     D L D  +SYYEWL    K   L+ + N  
Sbjct: 253 HEAVNVGGPNAVDISGL-DYPETMVVVAGFDPLKDWQRSYYEWLKLCGKKATLIEYPNMF 312

Query: 315 HGFSVFADLPEYSLMVKEIKDFI 335
           H F +F +LPE   ++  IKDF+
Sbjct: 313 HAFYIFPELPEAGQLIMRIKDFV 326

BLAST of Lsi10G006950 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 212.6 bits (540), Expect = 4.9e-55
Identity = 124/317 (39.12%), Postives = 175/317 (55.21%), Query Frame = 0

Query: 28  LLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRDLFLRIFTPTAAAA 87
           L +N   R + T NR L  F D K+P N N  + V S D+ ID   +L  R++ P  A  
Sbjct: 29  LAYNLLRRPDGTFNRHLAEFLDRKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGT 88

Query: 88  A---------ADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISVNYRLA 147
           +          D    PVI +FHGG FA  SA+S   D    R      AVV+SVNYR A
Sbjct: 89  SPSITDLQNPVDGEIVPVIVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRA 148

Query: 148 PEFRFPCQYDDGFDALKFIDEMDDSALMGRADLS-HCFILGESAGGNLGHHVAVRASEYA 207
           PE R+PC YDDG+  LK+++    S L  + D     F+ G+S+GGN+ H+VAVRA E  
Sbjct: 149 PENRYPCAYDDGWAVLKWVN--SSSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE-- 208

Query: 208 FKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRDHAAVNV 267
             R+ V+G I   P FGG ERTESE RL  +  +T++  DW+W+AFLP GE+R+H A + 
Sbjct: 209 -SRIDVLGNILLNPMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSP 268

Query: 268 NGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAVHGFSVF 327
            GP  + + G+ +FP +++    LDL+ D    Y E L K  ++VKL+    A  GF + 
Sbjct: 269 FGPRSKSLEGL-SFPKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYLL 328

Query: 328 ADLPEYSLMVKEIKDFI 335
            +   +  ++ EI  F+
Sbjct: 329 PNNNHFHTVMDEIAAFV 339

BLAST of Lsi10G006950 vs. TAIR 10
Match: AT3G05120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 202.6 bits (514), Expect = 5.0e-52
Identity = 120/317 (37.85%), Postives = 170/317 (53.63%), Query Frame = 0

Query: 30  FNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRDLFLRIFTPTAA---- 89
           +N   R + T NR L  + D K+  N N    V S D+ ID   +L  R++ P  A    
Sbjct: 31  YNILRRPDGTFNRHLAEYLDRKVTANANPVDGVFSFDVLIDRRINLLSRVYRPAYADQEQ 90

Query: 90  -------AAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISVNYRLA 149
                      D    PVI +FHGG FA  SA+S   D    R     + VV+SVNYR A
Sbjct: 91  PPSILDLEKPVDGDIVPVILFFHGGSFAHSSANSAIYDTLCRRLVGLCKCVVVSVNYRRA 150

Query: 150 PEFRFPCQYDDGFDALKFIDEMDDSALMGRADLS-HCFILGESAGGNLGHHVAVRASEYA 209
           PE  +PC YDDG+ AL +++    S L  + D   H F+ G+S+GGN+ H+VA+RA E  
Sbjct: 151 PENPYPCAYDDGWIALNWVNSR--SWLKSKKDSKVHIFLAGDSSGGNIAHNVALRAGE-- 210

Query: 210 FKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRDHAAVNV 269
              + V+G I   P FGG ERTESE  L  +  +T++  DW+WKAFLP GE+R+H A N 
Sbjct: 211 -SGIDVLGNILLNPMFGGNERTESEKSLDGKYFVTVRDRDWYWKAFLPEGEDREHPACNP 270

Query: 270 NGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAVHGFSVF 329
             P G+ + GV +FP +++    LDL+ D   +Y E L K  ++VKL+    A  GF + 
Sbjct: 271 FSPRGKSLEGV-SFPKSLVVVAGLDLIRDWQLAYAEGLKKAGQEVKLMHLEKATVGFYLL 330

Query: 330 ADLPEYSLMVKEIKDFI 335
            +   +  ++ EI  F+
Sbjct: 331 PNNNHFHNVMDEISAFV 341

BLAST of Lsi10G006950 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 189.9 bits (481), Expect = 3.4e-48
Identity = 110/319 (34.48%), Postives = 172/319 (53.92%), Query Frame = 0

Query: 28  LLFNTFLRSNLTVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRDLFLRIFTPTA--- 87
           L +    R + + NR L  F D K+P N      V S D  +D++ +L  RI+ P +   
Sbjct: 29  LAYKVLRRPDGSFNRDLAEFLDRKVPANSFPLDGVFSFD-HVDSTTNLLTRIYQPASLLH 88

Query: 88  ---------AAAAADSLPAPVIFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISVNY 147
                        + +   PV+ +FHGG F   SA+S   D    R       VV+SV+Y
Sbjct: 89  QTRHGTLELTKPLSTTEIVPVLIFFHGGSFTHSSANSAIYDTFCRRLVTICGVVVVSVDY 148

Query: 148 RLAPEFRFPCQYDDGFDALKFIDEMDDSALMGRADLSHCFILGESAGGNLGHHVAVRASE 207
           R +PE R+PC YDDG++AL ++ +       G+    + ++ G+S+GGN+ H+VAVRA+ 
Sbjct: 149 RRSPEHRYPCAYDDGWNALNWV-KSRVWLQSGKDSNVYVYLAGDSSGGNIAHNVAVRATN 208

Query: 208 YAFKRVKVVGFIASQPFFGGEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRDHAAV 267
              + VKV+G I   P FGG+ERT+SE  L  +  +T++  DW+W+A+LP GE+RDH A 
Sbjct: 209 ---EGVKVLGNILLHPMFGGQERTQSEKTLDGKYFVTIQDRDWYWRAYLPEGEDRDHPAC 268

Query: 268 NVNGPNGRDILGVRNFPATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAVHGFS 327
           N  GP G+ + GV NFP +++    LDL+ D   +Y + L K   +V L+    A  GF 
Sbjct: 269 NPFGPRGQSLKGV-NFPKSLVVVAGLDLVQDWQLAYVDGLKKTGLEVNLLYLKQATIGFY 328

Query: 328 VFADLPEYSLMVKEIKDFI 335
              +   +  +++E+  F+
Sbjct: 329 FLPNNDHFHCLMEELNKFV 341

BLAST of Lsi10G006950 vs. TAIR 10
Match: AT5G62180.1 (carboxyesterase 20 )

HSP 1 Score: 171.8 bits (434), Expect = 9.5e-43
Identity = 105/302 (34.77%), Postives = 158/302 (52.32%), Query Frame = 0

Query: 39  TVNRWLLNFFDPKIPPNPNLNHAVSSSDLTIDASRDLFLRIFTPTAAA--AAADSLPAPV 98
           ++ R L NF      P+P+  +   S DL ++  +  +LR++ P++A       S   P+
Sbjct: 23  SITRDLSNFPCTAATPDPSPLNPAVSKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQKLPI 82

Query: 99  IFYFHGGGFAIGSADSTSMDLAATRFAKELRAVVISVNYRLAPEFRFPCQYDDGFDALKF 158
           + Y+HGGGF + S D        +  A++L A+V+S +YRLAPE R P  YDDG +AL +
Sbjct: 83  VVYYHGGGFILCSVDMQLFHDFCSEVARDLNAIVVSPSYRLAPEHRLPAAYDDGVEALDW 142

Query: 159 IDEMDDSALMGRADLSHCFILGESAGGNLGHHVAVRA--SEYAFKRVKVVGFIASQPFFG 218
           I   DD  +   AD S+ F++G SAGGNL ++V +R+  S      +++ G I   PFFG
Sbjct: 143 IKTSDDEWIKSHADFSNVFLMGTSAGGNLAYNVGLRSVDSVSDLSPLQIRGLILHHPFFG 202

Query: 219 GEERTESEIRLCNEPLLTLKITDWFWKAFLPAGENRDHAAVNVNGPNGRDILGV--RNFP 278
           GEER+ESEIRL N+ +    +TD  W   LP G +RDH   N    +G + L    R   
Sbjct: 203 GEERSESEIRLMNDQVCPPIVTDVMWDLSLPVGVDRDHEYSNPTVGDGSEKLEKIGRLRW 262

Query: 279 ATVIFAGELDLLIDRHKSYYEWLMKMEKDVKLVVFSNAVHGFSVFADLPEYSLMVKEIKD 335
             ++  GE D +ID  K   + + K   +V        VHG  +  D  +   +   IK+
Sbjct: 263 KVMMIGGEDDPMIDLQKDVAKLMKKKGVEVVEHYTGGHVHGAEI-RDPSKRKTLFLSIKN 322

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LT102.6e-7747.37Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1[more]
Q940G66.8e-5439.12Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1[more]
Q6L5451.2e-5340.31Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 ... [more]
Q9MAA77.1e-5137.85Gibberellin receptor GID1A OS=Arabidopsis thaliana OX=3702 GN=GID1A PE=1 SV=1[more]
Q9LYC14.7e-4734.48Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1IER72.4e-15081.65probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111472040 PE=3 SV... [more]
A0A6J1ECC83.1e-15080.06probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111432777 PE=3 ... [more]
A0A0A0KXS87.1e-14777.81Acetyl esterase OS=Cucumis sativus OX=3659 GN=Csa_5G647390 PE=3 SV=1[more]
A0A1S3CBA41.8e-14576.85probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103498755 PE=3 SV=1[more]
A0A5D3BL918.2e-14375.67Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
Match NameE-valueIdentityDescription
XP_023536262.11.3e-15080.54probable carboxylesterase 18 [Cucurbita pepo subsp. pepo][more]
XP_022973499.14.9e-15081.65probable carboxylesterase 18 [Cucurbita maxima][more]
XP_022925491.16.4e-15080.06probable carboxylesterase 18 [Cucurbita moschata][more]
KAG6592331.13.2e-14980.79putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_004141618.11.5e-14677.81probable carboxylesterase 18 [Cucumis sativus] >KGN52616.1 hypothetical protein ... [more]
Match NameE-valueIdentityDescription
AT5G23530.11.8e-7847.37carboxyesterase 18 [more]
AT5G27320.14.9e-5539.12alpha/beta-Hydrolases superfamily protein [more]
AT3G05120.15.0e-5237.85alpha/beta-Hydrolases superfamily protein [more]
AT3G63010.13.4e-4834.48alpha/beta-Hydrolases superfamily protein [more]
AT5G62180.19.5e-4334.77carboxyesterase 20 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 4..335
e-value: 3.2E-83
score: 281.8
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 49..337
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 97..314
e-value: 1.9E-51
score: 174.9
NoneNo IPR availablePANTHERPTHR23024:SF434ACETYL ESTERASEcoord: 12..335
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 12..335
IPR002168Lipase, GDXG, putative histidine active sitePROSITEPS01173LIPASE_GDXG_HIScoord: 97..113

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G006950.1Lsi10G006950.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity