Lsi10G006600 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi10G006600 vs. ExPASy Swiss-Prot
Match: H6U1I8 (GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 1.7e-33
Identity = 83/189 (43.92%), Postives = 118/189 (62.43%), Query Frame = 0

Query: 35  IQLPDDVLSSGKQFGFFIFGDSYVDVGNNNYINATSDGQANFLPYGETFFPIPTGRFCDG 94
           + LP   LSS +    FIFGDS  D GNNN+IN   + +ANF PYG+++F  PTGRF DG
Sbjct: 19  LSLPTGCLSSQQAAALFIFGDSVFDPGNNNHINTHVNFKANFWPYGQSYFSSPTGRFSDG 78

Query: 95  RIIPDF-------------LDPNNNNYMNGVNFGSGGGGALVETNQQWVGIGLKTQMKFF 154
           RIIPDF             L+P NN++ +G NF S G GAL+  +   + +GL+TQ+++F
Sbjct: 79  RIIPDFIAEYASLPIIPAYLEP-NNDFTHGANFASAGAGALI-ASHAGLAVGLQTQLRYF 138

Query: 155 QEVEKSLRKKLGNESSQRFLSNSVFFFMFGGNDYQNPFDISYDIFKTIEAQEQYVNMVIG 211
            ++    R+ LG+  S++ LS++V+ F  GGNDYQ+P+   Y        QEQYV++VIG
Sbjct: 139 GDLVDHYRQNLGDIKSRQLLSDAVYLFSCGGNDYQSPY---YPY-----TQEQYVDIVIG 197

BLAST of Lsi10G006600 vs. ExPASy Swiss-Prot
Match: Q9FLN0 (GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.4e-32
Identity = 80/179 (44.69%), Postives = 110/179 (61.45%), Query Frame = 0

Query: 47  QFGFFIFGDSYVDVGNNNYINATSDGQANFLPYGETFFPIPTGRFCDGRIIPDF------ 106
           Q   F+FGDS  D GNNNYI+  S  ++N+ PYG+T F  PTGR  DGR+IPDF      
Sbjct: 35  QSALFVFGDSVFDAGNNNYIDTLSSVRSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYAW 94

Query: 107 ---LDPN------NNNYMNGVNFGSGGGGALVETNQQWVGIGLKTQMKFFQEVEKSLRKK 166
              + PN      N+ +  GVNF SGG GALV T    V I L+TQ+  F++VE+ LR K
Sbjct: 95  LPLIPPNLQPFNGNSQFAYGVNFASGGAGALVGTFSGLV-INLRTQLNNFKKVEEMLRSK 154

Query: 167 LGNESSQRFLSNSVFFFMFGGNDYQNPFDISYDIFKTIEAQEQYVNMVIGNITIALKVI 211
           LG+   +R +S +V+ F  G NDYQ PF  +  +F++I + E+YV+ V+GN+T   K +
Sbjct: 155 LGDAEGKRVISRAVYLFHIGLNDYQYPFTTNSSLFQSI-SNEKYVDYVVGNMTDVFKEV 211

BLAST of Lsi10G006600 vs. ExPASy Swiss-Prot
Match: Q9SSA7 (GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2)

HSP 1 Score: 132.9 bits (333), Expect = 5.0e-30
Identity = 89/216 (41.20%), Postives = 121/216 (56.02%), Query Frame = 0

Query: 12  FVFFFFYIFILFGIAAAQSSSRKIQLPDDVLSSGKQFGFFIFGDSYVDVGNNNYINATSD 71
           F+FF     ILF    A  SS KI    D          F+FGDS++D GNNNYIN T+ 
Sbjct: 18  FIFFIVSSTILF---LAGKSSAKISHNGD----NNVTALFLFGDSFLDAGNNNYINTTTL 77

Query: 72  GQANFLPYGETFFPIPTGRFCDGR-------------IIPDFLDP-NNNNYMNGVNFGSG 131
            QANF PYG+TFF +PTGRF DGR             +IP FL+P N+   + GVNF S 
Sbjct: 78  DQANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPLIPPFLEPGNSQKKLYGVNFASA 137

Query: 132 GGGALVETNQQWVGIGLKTQMKFFQEVEKSLRKKLGNESSQRFLSNSVFFFMFGGNDYQN 191
           G GALVET Q  V I L+TQ+  +++VE+  R   G E S++ +S +V+    G NDY +
Sbjct: 138 GAGALVETFQGSV-INLRTQLDHYKKVERLWRTNFGKEESKKRISRAVYLISIGSNDYSS 197

Query: 192 PFDISYDIFKTIEAQEQYVNMVIGNITIALKVISSV 214
            F  +  +     +  Q+V++VIGN+T  +  I  +
Sbjct: 198 IFLTNQSL---PISMSQHVDIVIGNLTTFIHEIYKI 222

BLAST of Lsi10G006600 vs. ExPASy Swiss-Prot
Match: Q9SYF5 (GDSL esterase/lipase 3 OS=Arabidopsis thaliana OX=3702 GN=GLIP3 PE=2 SV=2)

HSP 1 Score: 130.6 bits (327), Expect = 2.5e-29
Identity = 85/206 (41.26%), Postives = 113/206 (54.85%), Query Frame = 0

Query: 13  VFFFFYIFILFGIAAAQSSSRKIQLPDDVLSSGKQFGFFIFGDSYVDVGNNNYINATSDG 72
           + FF Y  IL        S   I   D+      Q   F+FGDS  D GNNNYIN  S  
Sbjct: 7   IIFFVYTIIL--------SIGSINCIDNNNLVTNQAALFVFGDSLFDAGNNNYINTVSSF 66

Query: 73  QANFLPYGETFFPIPTGRFCDG------RIIPDFLDPN--NNNYMNGVNFGSGGGGALVE 132
           ++N  PYG+T F  PTGR  DG        IP  L PN  NN +  GV+F S G GAL E
Sbjct: 67  RSNIWPYGQTNFKFPTGRLSDGPEKAWLPSIPPNLQPNNGNNQFTYGVSFASAGAGALAE 126

Query: 133 TNQQWVGIGLKTQMKFFQEVEKSLRKKLGNESSQRFLSNSVFFFMFGGNDYQNPFDISYD 192
           +    V I L TQ+  F++VEKSLR +LG+  ++R  S +V+ F  G NDY  PF  +  
Sbjct: 127 SFLGMV-INLGTQLNNFKDVEKSLRSELGDAETKRVFSRAVYLFHIGANDYFYPFSANSS 186

Query: 193 IFKTIEAQEQYVNMVIGNITIALKVI 211
            FK+  ++E++V+ VIGNIT  ++ +
Sbjct: 187 TFKS-NSKEKFVDFVIGNITFVIEEV 202

BLAST of Lsi10G006600 vs. ExPASy Swiss-Prot
Match: Q9SYF0 (GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 9.4e-29
Identity = 84/221 (38.01%), Postives = 116/221 (52.49%), Query Frame = 0

Query: 8   RCFFFVFFFFYIFILFGIAAAQSSSRKIQLPDDVLSSGKQFGFFIFGDSYVDVGNNNYIN 67
           R    +FF +   IL G    + ++    + +       Q   F+FGDS  D GNNNYI+
Sbjct: 5   RSTLIIFFAYTTIILIGSINCRDNNNNNLVTN-------QSALFVFGDSVFDAGNNNYID 64

Query: 68  ATSDGQANFLPYGETFFPIPTGRFCDGRIIPDF-------------LDPNN--NNYMNGV 127
                ++N+ PYG+T F  PTGR  DGR IPDF             L P+N  N +  GV
Sbjct: 65  TLPSFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPLIPAYLQPSNGKNQFPYGV 124

Query: 128 NFGSGGGGALVETNQQWVGIGLKTQMKFFQEVEKSLRKKLGNESSQRFLSNSVFFFMFGG 187
           +F S G GALV T    V I LK+Q+  F++VEK LR  LG    +  +S +V+ F  G 
Sbjct: 125 SFASAGAGALVGTFPGMV-INLKSQLNNFKKVEKLLRSTLGEAQGKMVISRAVYLFHIGV 184

Query: 188 NDYQNPFDISYDIFKTIEAQEQYVNMVIGNITIALKVISSV 214
           NDYQ PF  +  IF++   QE YV+ V+GN T  +K +  +
Sbjct: 185 NDYQYPFSTNSSIFQS-SPQEIYVDFVVGNTTAVIKEVYKI 216

BLAST of Lsi10G006600 vs. ExPASy TrEMBL
Match: A0A6J1EJ18 (GDSL esterase/lipase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111434897 PE=3 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 1.4e-64
Identity = 142/223 (63.68%), Postives = 159/223 (71.30%), Query Frame = 0

Query: 1   MKISNLGRCFFFVFFFFYIFILFGIAAAQSSSRKIQLPDDVLSSGKQFGFFIFGDSYVDV 60
           MKIS +G       FFF+ FIL GIAAA S    IQL  D+ SS  + GFFIFGDSYVD 
Sbjct: 1   MKISKMGS------FFFFFFILLGIAAAGSGFPGIQL-SDIPSSENRSGFFIFGDSYVDA 60

Query: 61  GNNNYINATSDGQANFLPYGETFFPIPTGRFCDGRIIPDF-------------LDPNNNN 120
           GNNNYIN T D  ANF PYGETFF  PTGRF DGRIIPDF             LDP NN 
Sbjct: 61  GNNNYINTTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPKNNL 120

Query: 121 YMNGVNFGSGGGGALVETNQQWVGIGLKTQMKFFQEVEKSLRKKLGNESSQRFLSNSVFF 180
           Y+NGVNF SGGGGAL  ++ Q   IGL+TQMKFF+ V+ SL K+LGN  +Q F SNSVF 
Sbjct: 121 YLNGVNFASGGGGALAGSH-QGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFL 180

Query: 181 FMFGGNDYQNPFDISYDIFKTIEAQEQYVNMVIGNITIALKVI 211
           F FGGNDY NPFDISYDIFKT++AQEQ+ +MVIGNITIALK +
Sbjct: 181 FNFGGNDYLNPFDISYDIFKTLDAQEQFADMVIGNITIALKEV 215

BLAST of Lsi10G006600 vs. ExPASy TrEMBL
Match: A0A6J1I3Y7 (GDSL esterase/lipase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111470363 PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 7.2e-64
Identity = 141/223 (63.23%), Postives = 159/223 (71.30%), Query Frame = 0

Query: 1   MKISNLGRCFFFVFFFFYIFILFGIAAAQSSSRKIQLPDDVLSSGKQFGFFIFGDSYVDV 60
           MKIS +G  FFF       FIL G AAA S    IQLP D+ SS  + GFFIFGDSYVD 
Sbjct: 1   MKISKMGSFFFF-------FILLGNAAAGSGFPGIQLP-DIPSSENRSGFFIFGDSYVDA 60

Query: 61  GNNNYINATSDGQANFLPYGETFFPIPTGRFCDGRIIPDF-------------LDPNNNN 120
           GNNNYIN T D  AN+ PYGETFF  PTGRF DGRIIPDF             LDP NN 
Sbjct: 61  GNNNYINTTGDFLANYPPYGETFFTTPTGRFSDGRIIPDFLAEYAKLPLIQPYLDPKNNL 120

Query: 121 YMNGVNFGSGGGGALVETNQQWVGIGLKTQMKFFQEVEKSLRKKLGNESSQRFLSNSVFF 180
           Y+NGVNF SGGGGAL E++ Q   IGL+TQMKFF+ V+ SL K+LG+  ++ F SNSVF 
Sbjct: 121 YLNGVNFASGGGGALAESH-QGQAIGLQTQMKFFKRVKNSLTKRLGSARTRSFFSNSVFL 180

Query: 181 FMFGGNDYQNPFDISYDIFKTIEAQEQYVNMVIGNITIALKVI 211
           F FGGNDY NPFDISYDIFKT++AQEQ+ NMVIGNITIALK +
Sbjct: 181 FNFGGNDYLNPFDISYDIFKTLDAQEQFANMVIGNITIALKEV 214

BLAST of Lsi10G006600 vs. ExPASy TrEMBL
Match: A0A6J1EIS0 (GDSL esterase/lipase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111434936 PE=3 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 2.7e-63
Identity = 143/229 (62.45%), Postives = 161/229 (70.31%), Query Frame = 0

Query: 1   MKISNLGRCFFFVFFFFYIFILFGIAAAQ------SSSRKIQLPDDVLSSGKQFGFFIFG 60
           MK  NLG CFFF+ F           AAQ      S +  IQLP    SS  +FGFFIFG
Sbjct: 1   MKFPNLGSCFFFILF-----------AAQCGFPTISEAVGIQLP-GAPSSENRFGFFIFG 60

Query: 61  DSYVDVGNNNYINATSDGQANFLPYGETFFPIPTGRFCDGRIIPDF-------------L 120
           DSYVDVGNNNYIN TSD QANF PYGE+FFP+ TGRF DGR IPDF             L
Sbjct: 61  DSYVDVGNNNYINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYL 120

Query: 121 DPNNNNYMNGVNFGSGGGGALVETNQQWVGIGLKTQMKFFQEVEKSLRKKLGNESSQRFL 180
           DP+N+ Y  GVNF SGGGGAL  ++Q+   +GL+TQMKFF+ V+KSLRKKLGN  +Q F 
Sbjct: 121 DPHNDLYDYGVNFASGGGGALALSHQE-QALGLQTQMKFFKRVDKSLRKKLGNARTQSFF 180

Query: 181 SNSVFFFMFGGNDYQNPFDISYDIFKTIEAQEQYVNMVIGNITIALKVI 211
           SNSVF F FGGNDY NPFDISYDIFKTI+AQEQ+VNMVIGNIT ALK +
Sbjct: 181 SNSVFLFNFGGNDYLNPFDISYDIFKTIDAQEQFVNMVIGNITKALKEV 216

BLAST of Lsi10G006600 vs. ExPASy TrEMBL
Match: A0A0A0LJN7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G296100 PE=3 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 2.3e-62
Identity = 137/224 (61.16%), Postives = 163/224 (72.77%), Query Frame = 0

Query: 1   MKISNLGRCFFFVFFFFYIFILFGIAAAQSSSRKIQLPDDVLSSGKQFGFFIFGDSYVDV 60
           MK  NLG CFFF       FILF IAAAQS+  KIQLP+  LS  K+FGFFIFG+S+VD 
Sbjct: 1   MKFPNLGICFFF------FFILFDIAAAQSAFLKIQLPNVPLSE-KRFGFFIFGNSFVDA 60

Query: 61  GNNNYINATSDGQANFLPYGETFFPIPTGRFCDGRIIPD-------------FLDPNNNN 120
           GNNNY+N T   ++NF PYGE+FFPIPTGR+CDGRIIPD             FLDPNN+N
Sbjct: 61  GNNNYLNGTIRTRSNFPPYGESFFPIPTGRYCDGRIIPDFLAEYAGMPFLPPFLDPNNSN 120

Query: 121 YMNGVNFGSGGGGALVETNQQWVGIGLKTQMKFFQEVEKSLRKKLGNES-SQRFLSNSVF 180
           YMNGVNFGSGG   L E+  +   + L+TQ++FF+ VEKS+RK +GNE+ SQ FLSNSVF
Sbjct: 121 YMNGVNFGSGGAPILPESTNE-TALSLQTQIEFFKIVEKSIRKDMGNETLSQTFLSNSVF 180

Query: 181 FFMFGGNDYQNPFDISYDIFKTIEAQEQYVNMVIGNITIALKVI 211
            F  GG D  +PF+ S+DIF TIE+QEQY NMVI N+TIALK I
Sbjct: 181 LFNIGGGDILHPFESSFDIFNTIESQEQYANMVINNMTIALKEI 216

BLAST of Lsi10G006600 vs. ExPASy TrEMBL
Match: A0A6J1KYX0 (GDSL esterase/lipase 2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499513 PE=3 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 3.7e-60
Identity = 137/231 (59.31%), Postives = 159/231 (68.83%), Query Frame = 0

Query: 1   MKISNLGRCFFFVFFFFYIFILFGIAAAQSSSRKIQLPDDVLSSGKQFGFFIFGDSYVDV 60
           MK+ NLG CF   FFFF +F L   +     +  I LP+        FGFF+FGDSYVD 
Sbjct: 1   MKLPNLGFCF---FFFFILFALQTASPTFPEASTISLPN-------CFGFFLFGDSYVDA 60

Query: 61  GNNNYINATSDGQANFLPYGETFFPIPTGRFCDGRIIPDF-------------LDPNNNN 120
           GNNNYIN T+D QANF PYG+TFF +PTGRF DGR IPDF             LDP+N+ 
Sbjct: 61  GNNNYINTTADFQANFPPYGKTFFHLPTGRFTDGRTIPDFLAEYAGLPLIPPYLDPHNDF 120

Query: 121 YMNGVNFGSGGGGALVETNQQWVGIGLKTQMKFFQEVEKSLRKKLGNESSQRFLSNSVFF 180
           Y NGVNF S G GAL E++ +   IGL+TQMKFF++V KSLRKKLGN S+Q  +SNSVF 
Sbjct: 121 YDNGVNFASSGSGALAESH-KGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSVFL 180

Query: 181 FMFGGNDYQNPFDISYDIFKTIEAQEQYVNMVIGNITIALKVISSVSKYVG 219
           F FGGNDY NPFDISYDIF T  AQE++VNMV+GNITIALK    V KY G
Sbjct: 181 FNFGGNDYLNPFDISYDIFNTSSAQERFVNMVVGNITIALK---EVYKYGG 217

BLAST of Lsi10G006600 vs. NCBI nr
Match: XP_038901398.1 (GDSL lipase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 305.4 bits (781), Expect = 4.3e-79
Identity = 167/223 (74.89%), Postives = 177/223 (79.37%), Query Frame = 0

Query: 1   MKISNLGRCFFFVFFFFYIFILFGIAAAQSSSRKIQLPDDVLSSGKQFGFFIFGDSYVDV 60
           MKISNLG C     FFFYIFILFGIAA QS+  KIQLP  V S GK+FGFFIFGDSYVD 
Sbjct: 1   MKISNLGIC----VFFFYIFILFGIAAPQSAFLKIQLP-HVQSWGKRFGFFIFGDSYVDA 60

Query: 61  GNNNYINATSDGQANFLPYGETFFPIPTGRFCDGRIIPDF-------------LDPNNNN 120
           GNNNYIN TSD +ANF PYGETFFPIPTGRF DGRIIPDF             LDPNNNN
Sbjct: 61  GNNNYINLTSDCRANFPPYGETFFPIPTGRFSDGRIIPDFLAEYAQMPFIQAYLDPNNNN 120

Query: 121 YMNGVNFGSGGGGALVETNQQWVGIGLKTQMKFFQEVEKSLRKKLGNESSQRFLSNSVFF 180
           Y NGVNF SGG GALVETNQ+   IGL+TQM FF+ V+KSLRKKLGNE SQ FLSNSVF 
Sbjct: 121 YTNGVNFASGGAGALVETNQK-KAIGLQTQMMFFKRVKKSLRKKLGNERSQSFLSNSVFL 180

Query: 181 FMFGGNDYQNPFDISYDIFKTIEAQEQYVNMVIGNITIALKVI 211
           F FGGNDY NP DI+YDIFKTIEAQEQ+VNMVIGNITIALK +
Sbjct: 181 FHFGGNDYLNPLDINYDIFKTIEAQEQFVNMVIGNITIALKEV 217

BLAST of Lsi10G006600 vs. NCBI nr
Match: XP_038901400.1 (GDSL lipase-like isoform X3 [Benincasa hispida])

HSP 1 Score: 305.4 bits (781), Expect = 4.3e-79
Identity = 167/223 (74.89%), Postives = 177/223 (79.37%), Query Frame = 0

Query: 1   MKISNLGRCFFFVFFFFYIFILFGIAAAQSSSRKIQLPDDVLSSGKQFGFFIFGDSYVDV 60
           MKISNLG C     FFFYIFILFGIAA QS+  KIQLP  V S GK+FGFFIFGDSYVD 
Sbjct: 1   MKISNLGIC----VFFFYIFILFGIAAPQSAFLKIQLP-HVQSWGKRFGFFIFGDSYVDA 60

Query: 61  GNNNYINATSDGQANFLPYGETFFPIPTGRFCDGRIIPDF-------------LDPNNNN 120
           GNNNYIN TSD +ANF PYGETFFPIPTGRF DGRIIPDF             LDPNNNN
Sbjct: 61  GNNNYINLTSDCRANFPPYGETFFPIPTGRFSDGRIIPDFLAEYAQMPFIQAYLDPNNNN 120

Query: 121 YMNGVNFGSGGGGALVETNQQWVGIGLKTQMKFFQEVEKSLRKKLGNESSQRFLSNSVFF 180
           Y NGVNF SGG GALVETNQ+   IGL+TQM FF+ V+KSLRKKLGNE SQ FLSNSVF 
Sbjct: 121 YTNGVNFASGGAGALVETNQK-KAIGLQTQMMFFKRVKKSLRKKLGNERSQSFLSNSVFL 180

Query: 181 FMFGGNDYQNPFDISYDIFKTIEAQEQYVNMVIGNITIALKVI 211
           F FGGNDY NP DI+YDIFKTIEAQEQ+VNMVIGNITIALK +
Sbjct: 181 FHFGGNDYLNPLDINYDIFKTIEAQEQFVNMVIGNITIALKEV 217

BLAST of Lsi10G006600 vs. NCBI nr
Match: XP_038901399.1 (GDSL esterase/lipase 3-like isoform X2 [Benincasa hispida])

HSP 1 Score: 295.4 bits (755), Expect = 4.4e-76
Identity = 161/215 (74.88%), Postives = 172/215 (80.00%), Query Frame = 0

Query: 1   MKISNLGRCFFFVFFFFYIFILFGIAAAQSSSRKIQLPDDVLSSGKQFGFFIFGDSYVDV 60
           MKISNLG C     FFFYIFILFGIAA QS+  KIQLP  V S GK+FGFFIFGDSYVD 
Sbjct: 1   MKISNLGIC----VFFFYIFILFGIAAPQSAFLKIQLP-HVQSWGKRFGFFIFGDSYVDA 60

Query: 61  GNNNYINATSDGQANFLPYGETFFPIPTGRFCDGR-----IIPDFLDPNNNNYMNGVNFG 120
           GNNNYIN TSD +ANF PYGETFFPIPTGRF D        I  +LDPNNNNY NGVNF 
Sbjct: 61  GNNNYINLTSDCRANFPPYGETFFPIPTGRFSDAEYAQMPFIQAYLDPNNNNYTNGVNFA 120

Query: 121 SGGGGALVETNQQWVGIGLKTQMKFFQEVEKSLRKKLGNESSQRFLSNSVFFFMFGGNDY 180
           SGG GALVETNQ+   IGL+TQM FF+ V+KSLRKKLGNE SQ FLSNSVF F FGGNDY
Sbjct: 121 SGGAGALVETNQK-KAIGLQTQMMFFKRVKKSLRKKLGNERSQSFLSNSVFLFHFGGNDY 180

Query: 181 QNPFDISYDIFKTIEAQEQYVNMVIGNITIALKVI 211
            NP DI+YDIFKTIEAQEQ+VNMVIGNITIALK +
Sbjct: 181 LNPLDINYDIFKTIEAQEQFVNMVIGNITIALKEV 209

BLAST of Lsi10G006600 vs. NCBI nr
Match: KAG6571388.1 (GDSL lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 258.1 bits (658), Expect = 7.9e-65
Identity = 139/221 (62.90%), Postives = 160/221 (72.40%), Query Frame = 0

Query: 12  FVFFFFYIFILFGIAAAQSSSRKIQLPDDVLSSGKQFGFFIFGDSYVDVGNNNYINATSD 71
           F FFFF+ F+L GI AA S    IQLP  + SS  +FGFFIFGDSYVD GNNNYIN T D
Sbjct: 4   FFFFFFFFFVLLGIGAAGSGFPGIQLP-GIPSSENRFGFFIFGDSYVDAGNNNYINTTGD 63

Query: 72  GQANFLPYGETFFPIPTGRFCDGRIIPDF-------------LDPNNNNYMNGVNFGSGG 131
             AN+ PYG+TFF  PTGRF DGRIIPDF             LDP NN Y+NGVNF SGG
Sbjct: 64  FLANYPPYGQTFFLTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPKNNLYLNGVNFASGG 123

Query: 132 GGALVETNQQWVGIGLKTQMKFFQEVEKSLRKKLGNESSQRFLSNSVFFFMFGGNDYQNP 191
           GGAL E++ Q   IGL+TQMKFF+ V+ SL K+LGN  +Q F SNSVF F FGGNDY NP
Sbjct: 124 GGALAESH-QGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFNFGGNDYLNP 183

Query: 192 FDISYDIFKTIEAQEQYVNMVIGNITIALKVISSVSKYVGS 220
           FDISYDIFKT++AQEQ+ +MVIGNITIALK    ++K + S
Sbjct: 184 FDISYDIFKTLDAQEQFADMVIGNITIALKTFEQLAKEMWS 222

BLAST of Lsi10G006600 vs. NCBI nr
Match: XP_023512753.1 (GDSL esterase/lipase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 256.5 bits (654), Expect = 2.3e-64
Identity = 137/210 (65.24%), Postives = 157/210 (74.76%), Query Frame = 0

Query: 14  FFFFYIFILFGIAAAQSSSRKIQLPDDVLSSGKQFGFFIFGDSYVDVGNNNYINATSDGQ 73
           FFFF++FIL GIAAA+S    IQLP  + SS  + GFFIFGDSYVD GNNNYIN T D  
Sbjct: 4   FFFFFVFILLGIAAAESGFPGIQLP-GIPSSENRSGFFIFGDSYVDAGNNNYINTTGDFL 63

Query: 74  ANFLPYGETFFPIPTGRFCDGRIIPDF-------------LDPNNNNYMNGVNFGSGGGG 133
           AN+ PYGETFFP PTGRF DGRIIPDF             LDP NN Y+NGVNF SGGGG
Sbjct: 64  ANYPPYGETFFPTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPTNNLYLNGVNFASGGGG 123

Query: 134 ALVETNQQWVGIGLKTQMKFFQEVEKSLRKKLGNESSQRFLSNSVFFFMFGGNDYQNPFD 193
           AL  ++ +   IGL+TQMKFF+ V+ SL K+LGN  +Q F SNSVF F FGGNDY NPFD
Sbjct: 124 ALAGSH-KGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFNFGGNDYLNPFD 183

Query: 194 ISYDIFKTIEAQEQYVNMVIGNITIALKVI 211
           ISYDIFKT++AQEQ+ +MVIGNITIALK +
Sbjct: 184 ISYDIFKTLDAQEQFADMVIGNITIALKEV 211

BLAST of Lsi10G006600 vs. TAIR 10
Match: AT5G40990.1 (GDSL lipase 1 )

HSP 1 Score: 140.6 bits (353), Expect = 1.7e-33
Identity = 80/179 (44.69%), Postives = 110/179 (61.45%), Query Frame = 0

Query: 47  QFGFFIFGDSYVDVGNNNYINATSDGQANFLPYGETFFPIPTGRFCDGRIIPDF------ 106
           Q   F+FGDS  D GNNNYI+  S  ++N+ PYG+T F  PTGR  DGR+IPDF      
Sbjct: 35  QSALFVFGDSVFDAGNNNYIDTLSSVRSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYAW 94

Query: 107 ---LDPN------NNNYMNGVNFGSGGGGALVETNQQWVGIGLKTQMKFFQEVEKSLRKK 166
              + PN      N+ +  GVNF SGG GALV T    V I L+TQ+  F++VE+ LR K
Sbjct: 95  LPLIPPNLQPFNGNSQFAYGVNFASGGAGALVGTFSGLV-INLRTQLNNFKKVEEMLRSK 154

Query: 167 LGNESSQRFLSNSVFFFMFGGNDYQNPFDISYDIFKTIEAQEQYVNMVIGNITIALKVI 211
           LG+   +R +S +V+ F  G NDYQ PF  +  +F++I + E+YV+ V+GN+T   K +
Sbjct: 155 LGDAEGKRVISRAVYLFHIGLNDYQYPFTTNSSLFQSI-SNEKYVDYVVGNMTDVFKEV 211

BLAST of Lsi10G006600 vs. TAIR 10
Match: AT1G53920.1 (GDSL-motif lipase 5 )

HSP 1 Score: 132.9 bits (333), Expect = 3.6e-31
Identity = 89/216 (41.20%), Postives = 121/216 (56.02%), Query Frame = 0

Query: 12  FVFFFFYIFILFGIAAAQSSSRKIQLPDDVLSSGKQFGFFIFGDSYVDVGNNNYINATSD 71
           F+FF     ILF    A  SS KI    D          F+FGDS++D GNNNYIN T+ 
Sbjct: 18  FIFFIVSSTILF---LAGKSSAKISHNGD----NNVTALFLFGDSFLDAGNNNYINTTTL 77

Query: 72  GQANFLPYGETFFPIPTGRFCDGR-------------IIPDFLDP-NNNNYMNGVNFGSG 131
            QANF PYG+TFF +PTGRF DGR             +IP FL+P N+   + GVNF S 
Sbjct: 78  DQANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPLIPPFLEPGNSQKKLYGVNFASA 137

Query: 132 GGGALVETNQQWVGIGLKTQMKFFQEVEKSLRKKLGNESSQRFLSNSVFFFMFGGNDYQN 191
           G GALVET Q  V I L+TQ+  +++VE+  R   G E S++ +S +V+    G NDY +
Sbjct: 138 GAGALVETFQGSV-INLRTQLDHYKKVERLWRTNFGKEESKKRISRAVYLISIGSNDYSS 197

Query: 192 PFDISYDIFKTIEAQEQYVNMVIGNITIALKVISSV 214
            F  +  +     +  Q+V++VIGN+T  +  I  +
Sbjct: 198 IFLTNQSL---PISMSQHVDIVIGNLTTFIHEIYKI 222

BLAST of Lsi10G006600 vs. TAIR 10
Match: AT1G53990.1 (GDSL-motif lipase 3 )

HSP 1 Score: 130.6 bits (327), Expect = 1.8e-30
Identity = 85/206 (41.26%), Postives = 113/206 (54.85%), Query Frame = 0

Query: 13  VFFFFYIFILFGIAAAQSSSRKIQLPDDVLSSGKQFGFFIFGDSYVDVGNNNYINATSDG 72
           + FF Y  IL        S   I   D+      Q   F+FGDS  D GNNNYIN  S  
Sbjct: 7   IIFFVYTIIL--------SIGSINCIDNNNLVTNQAALFVFGDSLFDAGNNNYINTVSSF 66

Query: 73  QANFLPYGETFFPIPTGRFCDG------RIIPDFLDPN--NNNYMNGVNFGSGGGGALVE 132
           ++N  PYG+T F  PTGR  DG        IP  L PN  NN +  GV+F S G GAL E
Sbjct: 67  RSNIWPYGQTNFKFPTGRLSDGPEKAWLPSIPPNLQPNNGNNQFTYGVSFASAGAGALAE 126

Query: 133 TNQQWVGIGLKTQMKFFQEVEKSLRKKLGNESSQRFLSNSVFFFMFGGNDYQNPFDISYD 192
           +    V I L TQ+  F++VEKSLR +LG+  ++R  S +V+ F  G NDY  PF  +  
Sbjct: 127 SFLGMV-INLGTQLNNFKDVEKSLRSELGDAETKRVFSRAVYLFHIGANDYFYPFSANSS 186

Query: 193 IFKTIEAQEQYVNMVIGNITIALKVI 211
            FK+  ++E++V+ VIGNIT  ++ +
Sbjct: 187 TFKS-NSKEKFVDFVIGNITFVIEEV 202

BLAST of Lsi10G006600 vs. TAIR 10
Match: AT1G53940.1 (GDSL-motif lipase 2 )

HSP 1 Score: 128.6 bits (322), Expect = 6.7e-30
Identity = 84/221 (38.01%), Postives = 116/221 (52.49%), Query Frame = 0

Query: 8   RCFFFVFFFFYIFILFGIAAAQSSSRKIQLPDDVLSSGKQFGFFIFGDSYVDVGNNNYIN 67
           R    +FF +   IL G    + ++    + +       Q   F+FGDS  D GNNNYI+
Sbjct: 5   RSTLIIFFAYTTIILIGSINCRDNNNNNLVTN-------QSALFVFGDSVFDAGNNNYID 64

Query: 68  ATSDGQANFLPYGETFFPIPTGRFCDGRIIPDF-------------LDPNN--NNYMNGV 127
                ++N+ PYG+T F  PTGR  DGR IPDF             L P+N  N +  GV
Sbjct: 65  TLPSFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPLIPAYLQPSNGKNQFPYGV 124

Query: 128 NFGSGGGGALVETNQQWVGIGLKTQMKFFQEVEKSLRKKLGNESSQRFLSNSVFFFMFGG 187
           +F S G GALV T    V I LK+Q+  F++VEK LR  LG    +  +S +V+ F  G 
Sbjct: 125 SFASAGAGALVGTFPGMV-INLKSQLNNFKKVEKLLRSTLGEAQGKMVISRAVYLFHIGV 184

Query: 188 NDYQNPFDISYDIFKTIEAQEQYVNMVIGNITIALKVISSV 214
           NDYQ PF  +  IF++   QE YV+ V+GN T  +K +  +
Sbjct: 185 NDYQYPFSTNSSIFQS-SPQEIYVDFVVGNTTAVIKEVYKI 216

BLAST of Lsi10G006600 vs. TAIR 10
Match: AT3G14225.1 (GDSL-motif lipase 4 )

HSP 1 Score: 109.8 bits (273), Expect = 3.2e-24
Identity = 73/211 (34.60%), Postives = 108/211 (51.18%), Query Frame = 0

Query: 16  FFYIFILFGIAAAQSSSRKIQLPDDVLSSGKQFGFFIFGDSYVDVGNNNYINATSDGQAN 75
           F  I I+  I     S   I   +D+ ++  Q   F FGDS  + GNNNY ++ S  ++N
Sbjct: 6   FNSIIIILFICTISLSIVSISCKEDLKTN--QAALFAFGDSLFEAGNNNYFDSISSFRSN 65

Query: 76  FLPYGETFFPIPTGRFCDGRIIPDF---------LDPN------NNNYMNGVNFGSGGGG 135
           F PYG+T F  PTGR  DGRI+ DF         + PN      N+    G+NF +   G
Sbjct: 66  FWPYGKTTFKFPTGRVSDGRIMIDFIAEYAWLPLIPPNLQPGYSNSQLTYGLNFATTAAG 125

Query: 136 ALVET---NQQWVGIGLKTQMKFFQEVEKSLRKKLGNESSQRFLSNSVFFFMFGGNDYQN 195
               T   +   +   L TQ+  F+ VEK+LR  LG+  ++R +S +V+ F  G NDYQ 
Sbjct: 126 VFAGTFPGSVTNLSKDLGTQLNNFKNVEKTLRSNLGDAEARRVISKAVYLFHIGANDYQY 185

Query: 196 PFDISYDIFKTIEAQEQYVNMVIGNITIALK 209
           PF  +   F     +E++++ VIGN T  ++
Sbjct: 186 PFFANTSTFSN-TTKERFIDFVIGNTTTVIE 213