Lsi10G006300 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G006300
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Descriptionphosphoglycerate mutase-like protein 4
Locationchr10: 8800155 .. 8806221 (+)
RNA-Seq ExpressionLsi10G006300
SyntenyLsi10G006300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATGGCTTCGGGCCTTACCTTGGACAACTCTCAACAAGTGTGCTACCTAAAAATTCTCAATAGAGAGGTTGAAGTGATAGAAATCTTTGGCACAAATTCGAACGGAACACATATTTTCAATGGGGCCAGATTCATCCTCATGAGCGCTCTGCGCTTCCATCTTCTTACTACTGCGACGCCCATTTCCGCTTCCAAGTCTTCATTGTTGTTCCTCAATTCTCCGCATCTAAAATCAACTTCATCTAAGAAGATCTTGGATCTCTTGATTTTCACTAGCCTCGAGACTTAACTCAGAAACGCAATCCCTTCCATGGCCGAGTCAGATTTCAGGTTCGATCATCATTTTTCTTTGCCCACCTTTCCTCCTATTGCCTATTTTTCTGGTTTCACCACAAATTTCGCAATTATTTTTCCTTTTTCTATTGTGAATTCTGAATTTTATAGATTGTCTTGAATTTGATTGCATGGTGACGTTGATATGCAGCTATATGAACCCAGCAGTTGCTGAAATTATTGTAATAAGGCACGGTGAAACAGCTTGGAATGCCGATGGAAGAATCCAGGTTCTACATCATATGCAAACCTATGAAATTTCGTTTAAATGAGTTTAATTAATTAATAACAAATAATCAAATGGGTATGATGATGATGTATTAAAGCTTCTGTTATGAGGAATAATTTCTCGGGTACAATGGCCTCATTTTTTTTTTAAACCTGGAATGGCTGAGTGCAGTAAGCTTGTAGGGTCGCATCACATATTACTTGTAAAAAAGGGGACAGAAGATTGGGGGGGGGGGGGAGGAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGGGGGGGGGGGGGAAGATAATAATTCAAAGTCATCTCAAATGAATTCAGTATAAGAGATTATGGAGTTTCTTTGAACTCTCACTGTTGTAATCTTTTTCATGATTGGTGAAATTTAGTGGTGAGTGGTTACACCTGTCAGGATTTTATGGGAGCAATTAGTTAATAGGTAATGTAAGCATAAAAGAAGATGAGGACAACAATAGAATCATGACGAGAAGGTTGGGGAGTTCAGTTTGCAGTCACAGAGGAACTTTCGCTAGTCAGTGATGAGTTGTCAAGTTCCATGGCAAGGATAGAATGTTTAAACAAGGAATAACAGTAGCAACAATATTTCAGGGTGACATGCAGAAGGGAAGGTGCAGATTGAAGGATCAGAAGAAACAACATAACCTGAAATTCTAAATGGATGATCCAGAAGCACATGAGCTAAGAACTGAGGAAAAATCATTAAGATGCTCATGTCTAAAGGAATTGACCCAAATGTGTGGACTTTTAGGAGTATTTCTCGATCAATTGATTTTCTAAAGAAGAAAAACTGGGGGTAGCCACTCTTTGCATGGAAGAGAAAAATCTGGCTTGGCATCAATGATCAATGTGAGGAAGGAAGGACAATGGCCCTTTCGGAGTTGGATTTACTTGAAATGGTAGCTAATTAAGTTAGAGCGACATAGGAAGGAAGCATTTGCAAGAAGTTCCTTTCTTTACCAAGTGTACATGTAGATAAATCTGCTGGAAATTTAAAACACTACCACACCATGCACCATTTGGAAGCATGTGTTAGAAGTGAAGTTTGTAAATGGGCTTAAAGGTGAGATTAAGGCACAAATGTGGGTCCTATAACCTGTGGATTTTGCCCAAAGATGGACCTACCCTAGATGGAATACAAGAATATCAAAATAGGCCTCCAATCAGGTTCATGTATATGAGGAGGAAAAAAGGTGAAGCAACGGGAAGGGGCTTAGCATGATTAAGAAAATTAGAGATGGGGCATCAAGGAAAATTGATGGAAAAAATGAATAAACGTGGCTTATGTAACACGTTGTTGTTTAGACCTTAGCTAGTCTGGTATGTTAGTTAGGGAGCCAATTAATTAAGCCTTGTACAGGTGGCAATCTAGTATTGGCTGTACCATATCAAAAAGGGAAAGGTTAGGGACGGAAAGATAGGCAAGGTTTTTGAGGAGATTGTACTTTCATTTTGGAAGAAGTGTAATATTAGAAGACAAAATCTTCTTCTATTTGGATTGATTGCTGTGGAAGAATGGTCAATTTTTAGGTATGGCAATTCTCTTACGGCAGGCATAATGCCATCAATCAATTTCAGAGGCATTCTTCCATTCTGTTGGTTGTTGGGGCTGCATTTTTGCGTTGTGTGTGAGAATGCTTGATCAAGTTTTGTCGAAGTGCTAACTTAACTTGCATGCTCAATTTGGAACAACTTTGATGATGGAGGTTTTCTTCTACCTGACGTTTTGAAATGGGGGAAAGGAAAGGCCTATTGGTATGTTGTAGCCTTCTCTACTACTTTATTGGATTATTGTGTGAATTAGTCCAGGTTCAAATCATTGAGTGAGCCTAATAAAGAAAACCAGTAATGTCTTGAGGGTTTGGGTTTTGAAGCAGGGGTAAACTTTGTAAGAAGAAAAAAGTTTTCAAATTTTGAAACTTAAAGAACCATACAGAATATTTAACTCAAACTCATCAACACTTAACACTTCACTTGTGGGTTTGAAATTTTTTTGAAGGCCTAACAAGTGGAATTCACTATTAATTAGGGAGGAAATGACTATAAAAATTTGAACTCAAGACCTTGCTTTGATCTCATGTTCAATCACCAATCAACCCAAAAGCTTAAGCTTATAGAGTAAATTTAATATTATATCAGTTATCAACACTCTAAGGCATGTATCCTTGATCAAAAAGTTAGAGGTTTGAATCTTCACCTTCACACCTTAACTAAAATCCTTACAAATGTTCTAGTGAATTGAATTTACCTATTATAACTTTTGTCACACTTGTTAATCTTGATTGTAAAATGTGCTTCTGATCATTTTCCTACTTCAACTTCGGGAAACGCTGCTATTACCATCTGAATTTTATGTTTTGAACAGGGACACCTGGACGTTGAATTAAATGAAGCTGGGCGACAGCAAGCAGTTGCAGTGAGTTAAAACTTATGTTTCATGTCTTAGTCTCAACATGTAACATAAAGGCTGTAAAACTATTTATATATTAAGGTATTTTGAACTTTTCAATCTTATTTTGCAGGTTGCTAATCGGCTGGCCAAGGAGCCTCCAATCTCTGCTGTATACTCTTCTGACTTGAAAAGAGCTCTTGAAACTGCACAGATAATTGCAACTACATGTGGTAATCTGGAGGTATGACTTCTGTCAGGCCATTTACAGTCACGGCTCTGTACTCAATTTTTCTGCTCTTTGTTCTTTTTTCTTCTCTTCCCTTTTCACCTAATTATATATAGTGGCTGTCACTCACTCGCTAACAGAAATGGTTGGTGGTCACTAACACTTATGTCCTGAAATCACTGAAATAACTTTGGACATTCTTGGGTTTTCAGTCTTCTTTTTAAGTTTTAACCCGGTATAATTGTTAATAAGTGAAATTTGATTGTTATTCTTTTTATTGTTGGAAATTTTATTGGAGATCACTTTTAGGTTATTAAAGATCCTGACTTGAGGGAAAGAAATTTAGGAGATCTTCAAGGTCTTGTGTATCGCGAGGCAGTCATAACAAACCTTGAAGCTACTGAGGCTCTGAGATCTCACAGGTCAGACCAAACAATTCCGGTAAGTAATAATATTTTATGCATATATCGATAGTAGGTATCTTGAAATCAGTGGAGCATTAAACCGGTTATTCATTAAATAAATCAGTTTTGAAGATCAAAGAACATTTTGGAAAGTCTACTGGGAGTAGCATGCAAAAAGCTTTTTTACTAACTGGTTCTGTCATTTTGTCTCCAAATTTATCCAGTATGCACACTTATTTTGCCAAAGTATTTAGTTATGAGCTGCATTTTTTTTTTTAAATTTTTAAGTGCCAGACTTTGGCCGCTTATGTTATGATGGGAGCTAAATTTTTTTTACAGGGATGGCCAAAGACCCCATTCCACGGTTCATCCCAAACCAAAATTAATTTAGAGCTGCATTTTTGTTGTTGTGGAGCCTAGGCAGTTGACCTCGGGTTTGGCACAAGATTTTTCCTGTTGTGAATTGACATATAAAGGAGGGGTTAACTTTTTCTTTAAAAAAAAATAATTTAGTCTAATTTTTTTTTGAAAAAATTAATTTAGTTTTAGAAAACTGAGAAACAAAAAGAAGAGATGAAGCGAAATAGATTATTACAAAACACGTTAGAAATTGAATCCCCCACAAAGGTGTTAAATGTAATGACCTCTCAAGACCTCTCTAAAAATATTGATTCTCTCTAACCAAACAACTGCTTGCGCCTGGCAAAAAAAAAAAAAAAAAAAGAAGAAGAAAAATCTACCTCTATCCTTGAAGGAAGGATGCAAGAGGACCTCCTTTAACATGACCCTCAAATTCCTATTACGTGGCAATCAAAATCTTGACGGTCTAAAAAGAAAAAATGATTTATAATTCCCATGTAAGATGGATGTGTTAAGTTACCACCTAACAAGAACACTCAAGAACAACAAAGAACACTAAAAGATAGAAATATATATTGTAATATCAAAAGATGCTACAATACCATAGCCTTTCGAAAGAGCTACACTCTCTCAAAATTTCCATCTTGAAAATTTTACTAAAATCATCACACCCTTTAAGGTCCTTAGCACACTATTTATAACCAAGACACCCCGGGCTAATGACCCTTATATCTTTACTATTATTATCCTACTAATAATCCTAATATATCTCTAACTAGGGCTCTCAGAGGATGTTTTAAGTTGACAACTATTGTCCACTACTGAACCTTTTATGTGCAGCAGAGTATGCATGTTATCGCTTGTAAATTTTGACCAATAGTGGAGAGATTAGGTCGTCTAGTAAATGTCTTAAGATATTTTGCATCTACAATATTCTGTCACATAGGATATTTACTCGAATTATTTACTTTACTATTGGCCCAAATGCAGGGTGGTGGAGAAAGTCTTGACCAACTTTATCAGCGTTGCACATCTTCATTGCAAAAGATTGGAAATAAGCATAGAGGTAATTACTCCCGTGGCTGAACATATTCACCTTGTCAAGGAAAATAAACAAGCATAAGAATAAGGAGAAAAACATCTCTTTGCAGTTGGGAAGTGCTCAATTTGAGGCTGAGTTTCTTTACTAATATTTGCTCGTTTAAAGCACAGGGCAACAAGTAGTTGTGGTCACTCATGGTGGTACCATCCGAGCGCTGTGTAAACGAGCTCATCCTCATAGCCACGGAGGGAAGATACTGAATACATCTGTCAATGTATTTCACTTGTCTGATGGAGACAAATGGAAGATAAAAACATGGGGTGATGTTAGCCATCTTGACCAGACAGATTATCTGCAGTCTGGATTTGGAGGAGATAAAAATTCTGGTTAGGTAGACGTTCCACATTGCATTATTGTGTCGTGTCGGTGCCATTTGATTCGAGCACAGGCAATTCACAATTCATTTTACTCGTCTGAGCGTTGACATGCTTGATCCTAGAGAGTTCATGCAAGGCCTGCCATTATCATACTATACTGGCCAAAATTAATAATGTTACATTTTTTTTTATTGTAATAACATTTAAAATGTGAACATTCGGATTCATGACCTATTAAGAAAGCGAAAATGAGCACTGTCATCAAGTGTATACTATTGGTTCAATCCTCCCACTCCACAATATTTGTC

mRNA sequence

TATGGCTTCGGGCCTTACCTTGGACAACTCTCAACAAGTGTGCTACCTAAAAATTCTCAATAGAGAGGTTGAAGTGATAGAAATCTTTGGCACAAATTCGAACGGAACACATATTTTCAATGGGGCCAGATTCATCCTCATGAGCGCTCTGCGCTTCCATCTTCTTACTACTGCGACGCCCATTTCCGCTTCCAAGTCTTCATTGTTGTTCCTCAATTCTCCGCATCTAAAATCAACTTCATCTAAGAAGATCTTGGATCTCTTGATTTTCACTAGCCTCGAGACTTAACTCAGAAACGCAATCCCTTCCATGGCCGAGTCAGATTTCAGCTATATGAACCCAGCAGTTGCTGAAATTATTGTAATAAGGCACGGTGAAACAGCTTGGAATGCCGATGGAAGAATCCAGGGACACCTGGACGTTGAATTAAATGAAGCTGGGCGACAGCAAGCAGTTGCAGTTGCTAATCGGCTGGCCAAGGAGCCTCCAATCTCTGCTGTATACTCTTCTGACTTGAAAAGAGCTCTTGAAACTGCACAGATAATTGCAACTACATGTGGTAATCTGGAGGTTATTAAAGATCCTGACTTGAGGGAAAGAAATTTAGGAGATCTTCAAGGTCTTGTGTATCGCGAGGCAGTCATAACAAACCTTGAAGCTACTGAGGCTCTGAGATCTCACAGGTCAGACCAAACAATTCCGGGTGGTGGAGAAAGTCTTGACCAACTTTATCAGCGTTGCACATCTTCATTGCAAAAGATTGGAAATAAGCATAGAGGGCAACAAGTAGTTGTGGTCACTCATGGTGGTACCATCCGAGCGCTGTGTAAACGAGCTCATCCTCATAGCCACGGAGGGAAGATACTGAATACATCTGTCAATGTATTTCACTTGTCTGATGGAGACAAATGGAAGATAAAAACATGGGGTGATGTTAGCCATCTTGACCAGACAGATTATCTGCAGTCTGGATTTGGAGGAGATAAAAATTCTGGTTAGGTAGACGTTCCACATTGCATTATTGTGTCGTGTCGGTGCCATTTGATTCGAGCACAGGCAATTCACAATTCATTTTACTCGTCTGAGCGTTGACATGCTTGATCCTAGAGAGTTCATGCAAGGCCTGCCATTATCATACTATACTGGCCAAAATTAATAATGTTACATTTTTTTTTATTGTAATAACATTTAAAATGTGAACATTCGGATTCATGACCTATTAAGAAAGCGAAAATGAGCACTGTCATCAAGTGTATACTATTGGTTCAATCCTCCCACTCCACAATATTTGTC

Coding sequence (CDS)

ATGGCCGAGTCAGATTTCAGCTATATGAACCCAGCAGTTGCTGAAATTATTGTAATAAGGCACGGTGAAACAGCTTGGAATGCCGATGGAAGAATCCAGGGACACCTGGACGTTGAATTAAATGAAGCTGGGCGACAGCAAGCAGTTGCAGTTGCTAATCGGCTGGCCAAGGAGCCTCCAATCTCTGCTGTATACTCTTCTGACTTGAAAAGAGCTCTTGAAACTGCACAGATAATTGCAACTACATGTGGTAATCTGGAGGTTATTAAAGATCCTGACTTGAGGGAAAGAAATTTAGGAGATCTTCAAGGTCTTGTGTATCGCGAGGCAGTCATAACAAACCTTGAAGCTACTGAGGCTCTGAGATCTCACAGGTCAGACCAAACAATTCCGGGTGGTGGAGAAAGTCTTGACCAACTTTATCAGCGTTGCACATCTTCATTGCAAAAGATTGGAAATAAGCATAGAGGGCAACAAGTAGTTGTGGTCACTCATGGTGGTACCATCCGAGCGCTGTGTAAACGAGCTCATCCTCATAGCCACGGAGGGAAGATACTGAATACATCTGTCAATGTATTTCACTTGTCTGATGGAGACAAATGGAAGATAAAAACATGGGGTGATGTTAGCCATCTTGACCAGACAGATTATCTGCAGTCTGGATTTGGAGGAGATAAAAATTCTGGTTAG

Protein sequence

MAESDFSYMNPAVAEIIVIRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPPISAVYSSDLKRALETAQIIATTCGNLEVIKDPDLRERNLGDLQGLVYREAVITNLEATEALRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQQVVVVTHGGTIRALCKRAHPHSHGGKILNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG
Homology
BLAST of Lsi10G006300 vs. ExPASy Swiss-Prot
Match: Q9SCS3 (Phosphoglycerate mutase-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At3g50520 PE=2 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 1.5e-81
Identity = 152/230 (66.09%), Postives = 184/230 (80.00%), Query Frame = 0

Query: 1   MAESDFSYMNPAVAEIIVIRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP 60
           M ES F   +   AEI+V+RHGET+WNA+ +IQGHLDVELN+AGRQQA  VA RL+KE  
Sbjct: 1   MEESRFDSEDVDYAEIVVVRHGETSWNAERKIQGHLDVELNDAGRQQAQRVAERLSKEQK 60

Query: 61  ISAVYSSDLKRALETAQIIATTCGNLEVIKDPDLRERNLGDLQGLVYREAVITNLEATEA 120
           IS VYSSDLKRA ETAQIIA  CG LEV+ D DLRER+LGD+QGLVY+EA     EA +A
Sbjct: 61  ISHVYSSDLKRAFETAQIIAAKCGKLEVLTDRDLRERHLGDMQGLVYQEASKIRPEAYKA 120

Query: 121 LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQQVVVVTHGGTIRALCKRAHPHS 180
             S+R+D  IPGGGESLD+LY RCT++LQ+IG+KH+G+++VVVTHGG IR+L +RA P +
Sbjct: 121 FSSNRTDVDIPGGGESLDKLYDRCTTALQRIGDKHKGERIVVVTHGGVIRSLYERARPSA 180

Query: 181 HG-GKILNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG 230
               KILNTSVNVF L DGDKW I+ WGDVSHL++T +LQSGFGGD+ SG
Sbjct: 181 RKVEKILNTSVNVFRLFDGDKWTIQVWGDVSHLEETGFLQSGFGGDRTSG 230

BLAST of Lsi10G006300 vs. ExPASy Swiss-Prot
Match: F4KI56 (Metal-independent phosphoserine phosphatase OS=Arabidopsis thaliana OX=3702 GN=IPSP PE=1 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 1.1e-58
Identity = 120/229 (52.40%), Postives = 153/229 (66.81%), Query Frame = 0

Query: 2   AESDFSY-----MNPAVAEIIVIRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLA 61
           AE +F +     +   V EI+++RHGET WNA GRIQG ++ +LNE G +QAVA+A RL 
Sbjct: 8   AEREFKWSEDVKVESEVTEIVLVRHGETTWNAAGRIQGQIESDLNEVGLKQAVAIAERLG 67

Query: 62  KEPPISAVYSSDLKRALETAQIIATTCGNLEVIKDPDLRERNLGDLQGLVYREAVITNLE 121
           KE    AVYSSDLKRA +TA +IA TC   EVI+ PDL+ER++G LQGL ++E      E
Sbjct: 68  KEERPVAVYSSDLKRAKDTALMIAKTCFCPEVIEVPDLKERHVGSLQGLYWKEGAEKEPE 127

Query: 122 ATEALRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQQVVVVTHGGTIRALCKRA 181
           A  A  S ++D  IPGGGES DQL  R   +L++I  KH+G++V+VVTHGG +RA+  R 
Sbjct: 128 AYSAFFSSQNDLEIPGGGESFDQLADRSMDALEQIAKKHKGERVIVVTHGGVLRAIYLRI 187

Query: 182 HPHSHGGKILNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGD 226
              S  GK+LN SVNV HL D  KW I +W DVSHL    +LQ GF GD
Sbjct: 188 TQASSAGKLLNASVNVVHLRD-QKWIIDSWSDVSHLSSVGFLQRGFDGD 235

BLAST of Lsi10G006300 vs. ExPASy Swiss-Prot
Match: A7ZVT7 (Probable phosphoglycerate mutase GpmB OS=Escherichia coli O139:H28 (strain E24377A / ETEC) OX=331111 GN=gpmB PE=3 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 8.8e-21
Identity = 74/213 (34.74%), Postives = 111/213 (52.11%), Query Frame = 0

Query: 15  EIIVIRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPPISAVYSSDLKRALE 74
           ++ ++RHGET WNA+ RIQG  D  L   G QQA+ VA R AKE  I+ + SSDL R   
Sbjct: 3   QVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATR-AKELGITHIISSDLGRTRR 62

Query: 75  TAQIIATTCGNLEVIKDPDLRERNLGDLQGLVYREAVITNLEATEALRSHRSDQTIPG-- 134
           TA+IIA  CG  ++I D  LRE N+G L+    +  + +  E  E  R    + T+ G  
Sbjct: 63  TAEIIAQACG-CDIIFDSRLRELNMGVLE----KRHIDSLTEEEENWRRQLVNGTVDGRI 122

Query: 135 -GGESLDQLYQRCTSSLQKIGNKHRGQQVVVVTHG-------GTIRALCKRAHPHSHGGK 194
             GES+ +L  R  ++L+   +  +G + ++V+HG        TI  L   A       +
Sbjct: 123 PEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVSTILGLPAWAERRL---R 182

Query: 195 ILNTSVNVFHLSD----GDKWKIKTWGDVSHLD 214
           + N S++     +       W ++T GD+SHLD
Sbjct: 183 LRNCSISRVDYQESLWLASGWVVETAGDISHLD 206

BLAST of Lsi10G006300 vs. ExPASy Swiss-Prot
Match: B7LEP1 (Probable phosphoglycerate mutase GpmB OS=Escherichia coli (strain 55989 / EAEC) OX=585055 GN=gpmB PE=3 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 8.8e-21
Identity = 74/213 (34.74%), Postives = 111/213 (52.11%), Query Frame = 0

Query: 15  EIIVIRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPPISAVYSSDLKRALE 74
           ++ ++RHGET WNA+ RIQG  D  L   G QQA+ VA R AKE  I+ + SSDL R   
Sbjct: 3   QVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATR-AKELGITHIISSDLGRTRR 62

Query: 75  TAQIIATTCGNLEVIKDPDLRERNLGDLQGLVYREAVITNLEATEALRSHRSDQTIPG-- 134
           TA+IIA  CG  ++I D  LRE N+G L+    +  + +  E  E  R    + T+ G  
Sbjct: 63  TAEIIAQACG-CDIIFDSRLRELNMGVLE----KRHIDSLTEEEENWRRQLVNGTVDGRI 122

Query: 135 -GGESLDQLYQRCTSSLQKIGNKHRGQQVVVVTHG-------GTIRALCKRAHPHSHGGK 194
             GES+ +L  R  ++L+   +  +G + ++V+HG        TI  L   A       +
Sbjct: 123 PEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVSTILGLPAWAERRL---R 182

Query: 195 ILNTSVNVFHLSD----GDKWKIKTWGDVSHLD 214
           + N S++     +       W ++T GD+SHLD
Sbjct: 183 LRNCSISRVDYQESLWLASGWVVETAGDISHLD 206

BLAST of Lsi10G006300 vs. ExPASy Swiss-Prot
Match: P0A7A3 (Probable phosphoglycerate mutase GpmB OS=Escherichia coli O157:H7 OX=83334 GN=gpmB PE=3 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 8.8e-21
Identity = 74/213 (34.74%), Postives = 111/213 (52.11%), Query Frame = 0

Query: 15  EIIVIRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPPISAVYSSDLKRALE 74
           ++ ++RHGET WNA+ RIQG  D  L   G QQA+ VA R AKE  I+ + SSDL R   
Sbjct: 3   QVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATR-AKELGITHIISSDLGRTRR 62

Query: 75  TAQIIATTCGNLEVIKDPDLRERNLGDLQGLVYREAVITNLEATEALRSHRSDQTIPG-- 134
           TA+IIA  CG  ++I D  LRE N+G L+    +  + +  E  E  R    + T+ G  
Sbjct: 63  TAEIIAQACG-CDIIFDSRLRELNMGVLE----KRHIDSLTEEEENWRRQLVNGTVDGRI 122

Query: 135 -GGESLDQLYQRCTSSLQKIGNKHRGQQVVVVTHG-------GTIRALCKRAHPHSHGGK 194
             GES+ +L  R  ++L+   +  +G + ++V+HG        TI  L   A       +
Sbjct: 123 PEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVSTILGLPAWAERRL---R 182

Query: 195 ILNTSVNVFHLSD----GDKWKIKTWGDVSHLD 214
           + N S++     +       W ++T GD+SHLD
Sbjct: 183 LRNCSISRVDYQESLWLASGWVVETAGDISHLD 206

BLAST of Lsi10G006300 vs. ExPASy TrEMBL
Match: A0A0A0LM12 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G292790 PE=4 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 1.1e-119
Identity = 215/231 (93.07%), Postives = 225/231 (97.40%), Query Frame = 0

Query: 1   MAESDFSYMNPAVAEIIVIRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP 60
           MA+ DFS++NPAVAEIIV+RHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP
Sbjct: 1   MADFDFSFVNPAVAEIIVVRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP 60

Query: 61  ISAVYSSDLKRALETAQIIATTCGNLEVIKDPDLRERNLGDLQGLVYREAVITNLEATEA 120
           +SAVYSSDLKRALETAQIIATTCGNLEVI DPDLRERNLGDLQGLVYREAV+TN EA+EA
Sbjct: 61  LSAVYSSDLKRALETAQIIATTCGNLEVITDPDLRERNLGDLQGLVYREAVLTNPEASEA 120

Query: 121 LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQQVVVVTHGGTIRALCKRAHPHS 180
           LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQ+VVVV+HGGTIRALCKRAHPH 
Sbjct: 121 LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQRVVVVSHGGTIRALCKRAHPHR 180

Query: 181 H--GGKILNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG 230
           H  GGK+LNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG
Sbjct: 181 HGGGGKVLNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG 231

BLAST of Lsi10G006300 vs. ExPASy TrEMBL
Match: A0A5D3CTF1 (Phosphoglycerate mutase-like protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G002540 PE=4 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 3.6e-118
Identity = 214/231 (92.64%), Postives = 224/231 (96.97%), Query Frame = 0

Query: 1   MAESDFSYMNPAVAEIIVIRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP 60
           MA+ D+S++NPAVAEIIV+RHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP
Sbjct: 61  MADFDYSFVNPAVAEIIVVRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP 120

Query: 61  ISAVYSSDLKRALETAQIIATTCGNLEVIKDPDLRERNLGDLQGLVYREAVITNLEATEA 120
           +SAVYSSDLKRALETAQIIATTCGNLEVI DPDLRERNLGDLQGLVYREAV+TN EA+EA
Sbjct: 121 LSAVYSSDLKRALETAQIIATTCGNLEVITDPDLRERNLGDLQGLVYREAVLTNPEASEA 180

Query: 121 LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQQVVVVTHGGTIRALCKRAHPHS 180
           LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQ+VVVV+HGGTIRAL KRAHPH 
Sbjct: 181 LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQRVVVVSHGGTIRALWKRAHPHR 240

Query: 181 H--GGKILNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG 230
           H  GGKILNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG
Sbjct: 241 HGGGGKILNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG 291

BLAST of Lsi10G006300 vs. ExPASy TrEMBL
Match: A0A1S3C624 (phosphoglycerate mutase-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103497447 PE=4 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 3.6e-118
Identity = 214/231 (92.64%), Postives = 224/231 (96.97%), Query Frame = 0

Query: 1   MAESDFSYMNPAVAEIIVIRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP 60
           MA+ D+S++NPAVAEIIV+RHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP
Sbjct: 61  MADFDYSFVNPAVAEIIVVRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP 120

Query: 61  ISAVYSSDLKRALETAQIIATTCGNLEVIKDPDLRERNLGDLQGLVYREAVITNLEATEA 120
           +SAVYSSDLKRALETAQIIATTCGNLEVI DPDLRERNLGDLQGLVYREAV+TN EA+EA
Sbjct: 121 LSAVYSSDLKRALETAQIIATTCGNLEVITDPDLRERNLGDLQGLVYREAVLTNPEASEA 180

Query: 121 LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQQVVVVTHGGTIRALCKRAHPHS 180
           LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQ+VVVV+HGGTIRAL KRAHPH 
Sbjct: 181 LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQRVVVVSHGGTIRALWKRAHPHR 240

Query: 181 H--GGKILNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG 230
           H  GGKILNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG
Sbjct: 241 HGGGGKILNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG 291

BLAST of Lsi10G006300 vs. ExPASy TrEMBL
Match: A0A6J1E0D3 (phosphoglycerate mutase-like protein 4 OS=Momordica charantia OX=3673 GN=LOC111024768 PE=4 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 8.6e-112
Identity = 205/230 (89.13%), Postives = 216/230 (93.91%), Query Frame = 0

Query: 1   MAESDFSYMNPAVAEIIVIRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP 60
           MA SDFSY+NPAVAEIIV+RHGETAWNADGRIQGHLDVELN+AGRQQAVAVANRLAKEP 
Sbjct: 62  MAGSDFSYVNPAVAEIIVVRHGETAWNADGRIQGHLDVELNDAGRQQAVAVANRLAKEPK 121

Query: 61  ISAVYSSDLKRALETAQIIATTCGNLEVIKDPDLRERNLGDLQGLVYREAVITNLEATEA 120
           ISAVYSSDLKRALETAQIIATTCGNLEVIKDPDLRER+LGDLQGLVYREAV T  EA  A
Sbjct: 122 ISAVYSSDLKRALETAQIIATTCGNLEVIKDPDLRERHLGDLQGLVYREAVTTKPEAWGA 181

Query: 121 LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQQVVVVTHGGTIRALCKRAHPH- 180
           LRSH SDQTIPGGGESLDQLYQRCTSSLQKIGNKHRG+QVVVVTHGGTIR+LCKRA  H 
Sbjct: 182 LRSHSSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGEQVVVVTHGGTIRSLCKRAQHHG 241

Query: 181 SHGGKILNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG 230
             GGKILNTSVN+FHLSDG+KWKIK+WGD+SHL QT+YLQSGFGGDKNSG
Sbjct: 242 DRGGKILNTSVNIFHLSDGEKWKIKSWGDISHLHQTEYLQSGFGGDKNSG 291

BLAST of Lsi10G006300 vs. ExPASy TrEMBL
Match: A0A6J1L413 (phosphoglycerate mutase-like protein 4 OS=Cucurbita maxima OX=3661 GN=LOC111499700 PE=4 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 1.4e-109
Identity = 197/229 (86.03%), Postives = 216/229 (94.32%), Query Frame = 0

Query: 1   MAESDFSYMNPAVAEIIVIRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP 60
           MAESDFSY+NPAVAEI+VIRHGETAWNA+ RIQGHLDVELNEAGRQQAVAVANRLAKE  
Sbjct: 1   MAESDFSYVNPAVAEIVVIRHGETAWNAEARIQGHLDVELNEAGRQQAVAVANRLAKERK 60

Query: 61  ISAVYSSDLKRALETAQIIATTCGNLEVIKDPDLRERNLGDLQGLVYREAVITNLEATEA 120
           ISAVYSSDLKRALETAQIIATTCG LEVIKDPDLRERNLGDLQGLVY E ++TN +A  A
Sbjct: 61  ISAVYSSDLKRALETAQIIATTCGILEVIKDPDLRERNLGDLQGLVYHEVIMTNPQAAAA 120

Query: 121 LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQQVVVVTHGGTIRALCKRAHPHS 180
           L+S R+DQTIPGGGESLDQLYQRCTSSLQ+IGNKHRG++VVVVTHGGTIRAL KR+HPH 
Sbjct: 121 LKSRRTDQTIPGGGESLDQLYQRCTSSLQQIGNKHRGERVVVVTHGGTIRALFKRSHPHG 180

Query: 181 HGGKILNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG 230
            GGKILNTSVN+FHLSDG++WK+K+WGDVSHL+QTDYLQSGFGGDK+SG
Sbjct: 181 IGGKILNTSVNIFHLSDGEEWKVKSWGDVSHLNQTDYLQSGFGGDKHSG 229

BLAST of Lsi10G006300 vs. NCBI nr
Match: XP_038900988.1 (phosphoglycerate mutase-like protein 4 isoform X1 [Benincasa hispida])

HSP 1 Score: 444.5 bits (1142), Expect = 5.5e-121
Identity = 218/229 (95.20%), Postives = 224/229 (97.82%), Query Frame = 0

Query: 1   MAESDFSYMNPAVAEIIVIRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP 60
           MAESDFSY+NPAVAEIIVIRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP
Sbjct: 1   MAESDFSYLNPAVAEIIVIRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP 60

Query: 61  ISAVYSSDLKRALETAQIIATTCGNLEVIKDPDLRERNLGDLQGLVYREAVITNLEATEA 120
           ISAVYSSDLKRALETAQIIATTCGNLEV KDPDLRERNLGDLQGLVYREAVITN EA+EA
Sbjct: 61  ISAVYSSDLKRALETAQIIATTCGNLEVTKDPDLRERNLGDLQGLVYREAVITNPEASEA 120

Query: 121 LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQQVVVVTHGGTIRALCKRAHPHS 180
           LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQ+VVVVTHGGTIRALCKRAHP  
Sbjct: 121 LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQRVVVVTHGGTIRALCKRAHPQK 180

Query: 181 HGGKILNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG 230
           HGGKILNTSVN+FHLSDGDKWKI+TWGDVSHL QT+YLQSGFGGDKNSG
Sbjct: 181 HGGKILNTSVNIFHLSDGDKWKIETWGDVSHLHQTNYLQSGFGGDKNSG 229

BLAST of Lsi10G006300 vs. NCBI nr
Match: XP_004148050.1 (phosphoglycerate mutase-like protein 4 [Cucumis sativus] >KGN62049.1 hypothetical protein Csa_006494 [Cucumis sativus])

HSP 1 Score: 439.1 bits (1128), Expect = 2.3e-119
Identity = 215/231 (93.07%), Postives = 225/231 (97.40%), Query Frame = 0

Query: 1   MAESDFSYMNPAVAEIIVIRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP 60
           MA+ DFS++NPAVAEIIV+RHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP
Sbjct: 1   MADFDFSFVNPAVAEIIVVRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP 60

Query: 61  ISAVYSSDLKRALETAQIIATTCGNLEVIKDPDLRERNLGDLQGLVYREAVITNLEATEA 120
           +SAVYSSDLKRALETAQIIATTCGNLEVI DPDLRERNLGDLQGLVYREAV+TN EA+EA
Sbjct: 61  LSAVYSSDLKRALETAQIIATTCGNLEVITDPDLRERNLGDLQGLVYREAVLTNPEASEA 120

Query: 121 LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQQVVVVTHGGTIRALCKRAHPHS 180
           LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQ+VVVV+HGGTIRALCKRAHPH 
Sbjct: 121 LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQRVVVVSHGGTIRALCKRAHPHR 180

Query: 181 H--GGKILNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG 230
           H  GGK+LNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG
Sbjct: 181 HGGGGKVLNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG 231

BLAST of Lsi10G006300 vs. NCBI nr
Match: XP_008457862.1 (PREDICTED: phosphoglycerate mutase-like protein 4 [Cucumis melo] >KAA0045862.1 phosphoglycerate mutase-like protein 4 [Cucumis melo var. makuwa] >TYK13726.1 phosphoglycerate mutase-like protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 434.1 bits (1115), Expect = 7.5e-118
Identity = 214/231 (92.64%), Postives = 224/231 (96.97%), Query Frame = 0

Query: 1   MAESDFSYMNPAVAEIIVIRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP 60
           MA+ D+S++NPAVAEIIV+RHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP
Sbjct: 61  MADFDYSFVNPAVAEIIVVRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP 120

Query: 61  ISAVYSSDLKRALETAQIIATTCGNLEVIKDPDLRERNLGDLQGLVYREAVITNLEATEA 120
           +SAVYSSDLKRALETAQIIATTCGNLEVI DPDLRERNLGDLQGLVYREAV+TN EA+EA
Sbjct: 121 LSAVYSSDLKRALETAQIIATTCGNLEVITDPDLRERNLGDLQGLVYREAVLTNPEASEA 180

Query: 121 LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQQVVVVTHGGTIRALCKRAHPHS 180
           LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQ+VVVV+HGGTIRAL KRAHPH 
Sbjct: 181 LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQRVVVVSHGGTIRALWKRAHPHR 240

Query: 181 H--GGKILNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG 230
           H  GGKILNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG
Sbjct: 241 HGGGGKILNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG 291

BLAST of Lsi10G006300 vs. NCBI nr
Match: XP_038900989.1 (phosphoglycerate mutase-like protein 4 isoform X2 [Benincasa hispida])

HSP 1 Score: 429.1 bits (1102), Expect = 2.4e-116
Identity = 218/258 (84.50%), Postives = 224/258 (86.82%), Query Frame = 0

Query: 1   MAESDFSYMNPAVAEIIVIRHGETAWNADGRIQ--------------------------- 60
           MAESDFSY+NPAVAEIIVIRHGETAWNADGRIQ                           
Sbjct: 1   MAESDFSYLNPAVAEIIVIRHGETAWNADGRIQVWQFSHWRHNAIDQFQRHSSILLAVGA 60

Query: 61  --GHLDVELNEAGRQQAVAVANRLAKEPPISAVYSSDLKRALETAQIIATTCGNLEVIKD 120
             GHLDVELNEAGRQQAVAVANRLAKEPPISAVYSSDLKRALETAQIIATTCGNLEV KD
Sbjct: 61  TWGHLDVELNEAGRQQAVAVANRLAKEPPISAVYSSDLKRALETAQIIATTCGNLEVTKD 120

Query: 121 PDLRERNLGDLQGLVYREAVITNLEATEALRSHRSDQTIPGGGESLDQLYQRCTSSLQKI 180
           PDLRERNLGDLQGLVYREAVITN EA+EALRSHRSDQTIPGGGESLDQLYQRCTSSLQKI
Sbjct: 121 PDLRERNLGDLQGLVYREAVITNPEASEALRSHRSDQTIPGGGESLDQLYQRCTSSLQKI 180

Query: 181 GNKHRGQQVVVVTHGGTIRALCKRAHPHSHGGKILNTSVNVFHLSDGDKWKIKTWGDVSH 230
           GNKHRGQ+VVVVTHGGTIRALCKRAHP  HGGKILNTSVN+FHLSDGDKWKI+TWGDVSH
Sbjct: 181 GNKHRGQRVVVVTHGGTIRALCKRAHPQKHGGKILNTSVNIFHLSDGDKWKIETWGDVSH 240

BLAST of Lsi10G006300 vs. NCBI nr
Match: XP_022158236.1 (phosphoglycerate mutase-like protein 4 [Momordica charantia])

HSP 1 Score: 412.9 bits (1060), Expect = 1.8e-111
Identity = 205/230 (89.13%), Postives = 216/230 (93.91%), Query Frame = 0

Query: 1   MAESDFSYMNPAVAEIIVIRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP 60
           MA SDFSY+NPAVAEIIV+RHGETAWNADGRIQGHLDVELN+AGRQQAVAVANRLAKEP 
Sbjct: 62  MAGSDFSYVNPAVAEIIVVRHGETAWNADGRIQGHLDVELNDAGRQQAVAVANRLAKEPK 121

Query: 61  ISAVYSSDLKRALETAQIIATTCGNLEVIKDPDLRERNLGDLQGLVYREAVITNLEATEA 120
           ISAVYSSDLKRALETAQIIATTCGNLEVIKDPDLRER+LGDLQGLVYREAV T  EA  A
Sbjct: 122 ISAVYSSDLKRALETAQIIATTCGNLEVIKDPDLRERHLGDLQGLVYREAVTTKPEAWGA 181

Query: 121 LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQQVVVVTHGGTIRALCKRAHPH- 180
           LRSH SDQTIPGGGESLDQLYQRCTSSLQKIGNKHRG+QVVVVTHGGTIR+LCKRA  H 
Sbjct: 182 LRSHSSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGEQVVVVTHGGTIRSLCKRAQHHG 241

Query: 181 SHGGKILNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG 230
             GGKILNTSVN+FHLSDG+KWKIK+WGD+SHL QT+YLQSGFGGDKNSG
Sbjct: 242 DRGGKILNTSVNIFHLSDGEKWKIKSWGDISHLHQTEYLQSGFGGDKNSG 291

BLAST of Lsi10G006300 vs. TAIR 10
Match: AT3G50520.1 (Phosphoglycerate mutase family protein )

HSP 1 Score: 303.9 bits (777), Expect = 1.1e-82
Identity = 152/230 (66.09%), Postives = 184/230 (80.00%), Query Frame = 0

Query: 1   MAESDFSYMNPAVAEIIVIRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLAKEPP 60
           M ES F   +   AEI+V+RHGET+WNA+ +IQGHLDVELN+AGRQQA  VA RL+KE  
Sbjct: 1   MEESRFDSEDVDYAEIVVVRHGETSWNAERKIQGHLDVELNDAGRQQAQRVAERLSKEQK 60

Query: 61  ISAVYSSDLKRALETAQIIATTCGNLEVIKDPDLRERNLGDLQGLVYREAVITNLEATEA 120
           IS VYSSDLKRA ETAQIIA  CG LEV+ D DLRER+LGD+QGLVY+EA     EA +A
Sbjct: 61  ISHVYSSDLKRAFETAQIIAAKCGKLEVLTDRDLRERHLGDMQGLVYQEASKIRPEAYKA 120

Query: 121 LRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQQVVVVTHGGTIRALCKRAHPHS 180
             S+R+D  IPGGGESLD+LY RCT++LQ+IG+KH+G+++VVVTHGG IR+L +RA P +
Sbjct: 121 FSSNRTDVDIPGGGESLDKLYDRCTTALQRIGDKHKGERIVVVTHGGVIRSLYERARPSA 180

Query: 181 HG-GKILNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGDKNSG 230
               KILNTSVNVF L DGDKW I+ WGDVSHL++T +LQSGFGGD+ SG
Sbjct: 181 RKVEKILNTSVNVFRLFDGDKWTIQVWGDVSHLEETGFLQSGFGGDRTSG 230

BLAST of Lsi10G006300 vs. TAIR 10
Match: AT5G04120.1 (Phosphoglycerate mutase family protein )

HSP 1 Score: 228.0 bits (580), Expect = 7.6e-60
Identity = 120/229 (52.40%), Postives = 153/229 (66.81%), Query Frame = 0

Query: 2   AESDFSY-----MNPAVAEIIVIRHGETAWNADGRIQGHLDVELNEAGRQQAVAVANRLA 61
           AE +F +     +   V EI+++RHGET WNA GRIQG ++ +LNE G +QAVA+A RL 
Sbjct: 8   AEREFKWSEDVKVESEVTEIVLVRHGETTWNAAGRIQGQIESDLNEVGLKQAVAIAERLG 67

Query: 62  KEPPISAVYSSDLKRALETAQIIATTCGNLEVIKDPDLRERNLGDLQGLVYREAVITNLE 121
           KE    AVYSSDLKRA +TA +IA TC   EVI+ PDL+ER++G LQGL ++E      E
Sbjct: 68  KEERPVAVYSSDLKRAKDTALMIAKTCFCPEVIEVPDLKERHVGSLQGLYWKEGAEKEPE 127

Query: 122 ATEALRSHRSDQTIPGGGESLDQLYQRCTSSLQKIGNKHRGQQVVVVTHGGTIRALCKRA 181
           A  A  S ++D  IPGGGES DQL  R   +L++I  KH+G++V+VVTHGG +RA+  R 
Sbjct: 128 AYSAFFSSQNDLEIPGGGESFDQLADRSMDALEQIAKKHKGERVIVVTHGGVLRAIYLRI 187

Query: 182 HPHSHGGKILNTSVNVFHLSDGDKWKIKTWGDVSHLDQTDYLQSGFGGD 226
              S  GK+LN SVNV HL D  KW I +W DVSHL    +LQ GF GD
Sbjct: 188 TQASSAGKLLNASVNVVHLRD-QKWIIDSWSDVSHLSSVGFLQRGFDGD 235

BLAST of Lsi10G006300 vs. TAIR 10
Match: AT5G22620.1 (phosphoglycerate/bisphosphoglycerate mutase family protein )

HSP 1 Score: 67.0 bits (162), Expect = 2.2e-11
Identity = 52/164 (31.71%), Postives = 84/164 (51.22%), Query Frame = 0

Query: 16  IIVIRHGETAWNADGRIQGHLDVE-LNEAGRQQAVAVANRLAKEPPISAVYSSDLKRALE 75
           ++++RHG++ WN +GRIQG  D   L + G  QA  ++ ++  +      ++S LKR+ +
Sbjct: 50  VVLVRHGQSTWNEEGRIQGSSDFSVLTKKGESQA-EISRQMLIDDSFDVCFTSPLKRSKK 109

Query: 76  TAQIIATTCGNLEVIKDPDLRERNLGDLQGLVYREAVITNLEATEALRSHRSDQT--IPG 135
           TA+II  +    E+I D DLRE +L   QGL+ +E      +  EA +  + D    I  
Sbjct: 110 TAEIIWGS-RESEMIFDYDLREIDLYSFQGLLKKEG---KEKFGEAFKQWQEDPANFIID 169

Query: 136 GGESLDQLYQRCTSSLQKIGNKHRGQQVVVVTHGGTIRALCKRA 177
           G   + +L+ R  S    I   H  + V+VV H    +AL   A
Sbjct: 170 GHYPVRELWSRARSCWPGI-LAHESKSVLVVAHNAVNQALLATA 207

BLAST of Lsi10G006300 vs. TAIR 10
Match: AT5G22620.2 (phosphoglycerate/bisphosphoglycerate mutase family protein )

HSP 1 Score: 67.0 bits (162), Expect = 2.2e-11
Identity = 52/164 (31.71%), Postives = 84/164 (51.22%), Query Frame = 0

Query: 16  IIVIRHGETAWNADGRIQGHLDVE-LNEAGRQQAVAVANRLAKEPPISAVYSSDLKRALE 75
           ++++RHG++ WN +GRIQG  D   L + G  QA  ++ ++  +      ++S LKR+ +
Sbjct: 50  VVLVRHGQSTWNEEGRIQGSSDFSVLTKKGESQA-EISRQMLIDDSFDVCFTSPLKRSKK 109

Query: 76  TAQIIATTCGNLEVIKDPDLRERNLGDLQGLVYREAVITNLEATEALRSHRSDQT--IPG 135
           TA+II  +    E+I D DLRE +L   QGL+ +E      +  EA +  + D    I  
Sbjct: 110 TAEIIWGS-RESEMIFDYDLREIDLYSFQGLLKKEG---KEKFGEAFKQWQEDPANFIID 169

Query: 136 GGESLDQLYQRCTSSLQKIGNKHRGQQVVVVTHGGTIRALCKRA 177
           G   + +L+ R  S    I   H  + V+VV H    +AL   A
Sbjct: 170 GHYPVRELWSRARSCWPGI-LAHESKSVLVVAHNAVNQALLATA 207

BLAST of Lsi10G006300 vs. TAIR 10
Match: AT5G22620.3 (phosphoglycerate/bisphosphoglycerate mutase family protein )

HSP 1 Score: 67.0 bits (162), Expect = 2.2e-11
Identity = 52/164 (31.71%), Postives = 84/164 (51.22%), Query Frame = 0

Query: 16  IIVIRHGETAWNADGRIQGHLDVE-LNEAGRQQAVAVANRLAKEPPISAVYSSDLKRALE 75
           ++++RHG++ WN +GRIQG  D   L + G  QA  ++ ++  +      ++S LKR+ +
Sbjct: 50  VVLVRHGQSTWNEEGRIQGSSDFSVLTKKGESQA-EISRQMLIDDSFDVCFTSPLKRSKK 109

Query: 76  TAQIIATTCGNLEVIKDPDLRERNLGDLQGLVYREAVITNLEATEALRSHRSDQT--IPG 135
           TA+II  +    E+I D DLRE +L   QGL+ +E      +  EA +  + D    I  
Sbjct: 110 TAEIIWGS-RESEMIFDYDLREIDLYSFQGLLKKEG---KEKFGEAFKQWQEDPANFIID 169

Query: 136 GGESLDQLYQRCTSSLQKIGNKHRGQQVVVVTHGGTIRALCKRA 177
           G   + +L+ R  S    I   H  + V+VV H    +AL   A
Sbjct: 170 GHYPVRELWSRARSCWPGI-LAHESKSVLVVAHNAVNQALLATA 207

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SCS31.5e-8166.09Phosphoglycerate mutase-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At3g50... [more]
F4KI561.1e-5852.40Metal-independent phosphoserine phosphatase OS=Arabidopsis thaliana OX=3702 GN=I... [more]
A7ZVT78.8e-2134.74Probable phosphoglycerate mutase GpmB OS=Escherichia coli O139:H28 (strain E2437... [more]
B7LEP18.8e-2134.74Probable phosphoglycerate mutase GpmB OS=Escherichia coli (strain 55989 / EAEC) ... [more]
P0A7A38.8e-2134.74Probable phosphoglycerate mutase GpmB OS=Escherichia coli O157:H7 OX=83334 GN=gp... [more]
Match NameE-valueIdentityDescription
A0A0A0LM121.1e-11993.07Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G292790 PE=4 SV=1[more]
A0A5D3CTF13.6e-11892.64Phosphoglycerate mutase-like protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3C6243.6e-11892.64phosphoglycerate mutase-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103497447 P... [more]
A0A6J1E0D38.6e-11289.13phosphoglycerate mutase-like protein 4 OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A6J1L4131.4e-10986.03phosphoglycerate mutase-like protein 4 OS=Cucurbita maxima OX=3661 GN=LOC1114997... [more]
Match NameE-valueIdentityDescription
XP_038900988.15.5e-12195.20phosphoglycerate mutase-like protein 4 isoform X1 [Benincasa hispida][more]
XP_004148050.12.3e-11993.07phosphoglycerate mutase-like protein 4 [Cucumis sativus] >KGN62049.1 hypothetica... [more]
XP_008457862.17.5e-11892.64PREDICTED: phosphoglycerate mutase-like protein 4 [Cucumis melo] >KAA0045862.1 p... [more]
XP_038900989.12.4e-11684.50phosphoglycerate mutase-like protein 4 isoform X2 [Benincasa hispida][more]
XP_022158236.11.8e-11189.13phosphoglycerate mutase-like protein 4 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT3G50520.11.1e-8266.09Phosphoglycerate mutase family protein [more]
AT5G04120.17.6e-6052.40Phosphoglycerate mutase family protein [more]
AT5G22620.12.2e-1131.71phosphoglycerate/bisphosphoglycerate mutase family protein [more]
AT5G22620.22.2e-1131.71phosphoglycerate/bisphosphoglycerate mutase family protein [more]
AT5G22620.32.2e-1131.71phosphoglycerate/bisphosphoglycerate mutase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013078Histidine phosphatase superfamily, clade-1SMARTSM00855PGAM_5coord: 15..172
e-value: 3.2E-25
score: 99.8
IPR013078Histidine phosphatase superfamily, clade-1PFAMPF00300His_Phos_1coord: 16..208
e-value: 8.3E-58
score: 195.3
IPR013078Histidine phosphatase superfamily, clade-1CDDcd07067HP_PGM_likecoord: 15..206
e-value: 1.9325E-41
score: 136.683
IPR029033Histidine phosphatase superfamilyGENE3D3.40.50.1240coord: 8..218
e-value: 9.8E-64
score: 217.2
IPR029033Histidine phosphatase superfamilySUPERFAMILY53254Phosphoglycerate mutase-likecoord: 15..214
NoneNo IPR availablePANTHERPTHR48100:SF35BNAC07G31810D PROTEINcoord: 1..227
NoneNo IPR availablePANTHERPTHR48100BROAD-SPECIFICITY PHOSPHATASE YOR283W-RELATEDcoord: 1..227
IPR001345Phosphoglycerate/bisphosphoglycerate mutase, active sitePROSITEPS00175PG_MUTASEcoord: 18..27

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G006300.1Lsi10G006300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016311 dephosphorylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016868 intramolecular transferase activity, phosphotransferases
molecular_function GO:0016791 phosphatase activity
molecular_function GO:0003824 catalytic activity