Lsi10G006260 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G006260
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionDisease resistance protein
Locationchr10: 8745381 .. 8746307 (-)
RNA-Seq ExpressionLsi10G006260
SyntenyLsi10G006260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGCTATAGTTGGAGTATTGGCAGATTATACAATTAAACCTATTTTACATCAATTAGGTTACCTCTTTCTCTACAACATAAATCTTAAGGAAGTTGAAAAGAAACTTGAAACACTTAGGACTACTAAAGATAGCGTGCAAATTTTGGTTAGTCAAGATAAAGGCAAAGGGAATGCAATCTTCGATGAAGTTACCAAGTGGTTGGACAATGTAGAGAATGTGCTTGACATGGCACAACAAAACGAAGGCCCTAATCCCTCGTGCTTTAATTTTGTCAAACGACATCAGTCATCCAGAAAAGCAAAGAAGAGAGTTGAAAATATTATTGAACTCATAAATGAAGAAAGACAATTTAACAAAGATAATGTCGGTTATCCTGTACCTTCTCCAGATACTAATTCTCCTACTCTCCCTGCAGATTACCAAATTATAAAATCAAGAACATCAATGCCTAAGAAAATCAAGGATGCACTTGCAGATCCTAATGTCAACAAGGTTGGAGTATGTGGTATGGGAGGTGTTGGAAAAACTGCACTCTTGAATGAAGTTAAGAAGTTAGTATTGGAAAACAATTTGTTTGATCGAGTAATTCAGGTGGAAGTGGGTCAATCCAAGAGTGTAATCCAAATTCAAGAAGGAATTGGAGGGAAGTTAAATATAGATTTAAGTACGCAGTGTAAGGAGGTAAGAGCATCTCGTCTACGAACTCACATTGCTGAGAGGAAGGAGAATATCCTCATTATGCTGGATGATTTATGGAAGGAATATGATCTAGAAAATGATTTTAGAATTTCGTGTCATTCAGAATCAAGGATAGGAGGGTGCAAGATACTTATGACAAGTCGATCTCAACATACATTAAAGAATGAAATGAATACAGAAAAGTTATTTGAAGTGAATTGTCTAACTAAAGAAGAGTCTTGA

mRNA sequence

ATGGATGCTATAGTTGGAGTATTGGCAGATTATACAATTAAACCTATTTTACATCAATTAGGTTACCTCTTTCTCTACAACATAAATCTTAAGGAAGTTGAAAAGAAACTTGAAACACTTAGGACTACTAAAGATAGCGTGCAAATTTTGGTTAGTCAAGATAAAGGCAAAGGGAATGCAATCTTCGATGAAGTTACCAAGTGGTTGGACAATGTAGAGAATGTGCTTGACATGGCACAACAAAACGAAGGCCCTAATCCCTCGTGCTTTAATTTTGTCAAACGACATCAGTCATCCAGAAAAGCAAAGAAGAGAGTTGAAAATATTATTGAACTCATAAATGAAGAAAGACAATTTAACAAAGATAATGTCGGTTATCCTGTACCTTCTCCAGATACTAATTCTCCTACTCTCCCTGCAGATTACCAAATTATAAAATCAAGAACATCAATGCCTAAGAAAATCAAGGATGCACTTGCAGATCCTAATGTCAACAAGGTTGGAGTATGTGGTATGGGAGGTGTTGGAAAAACTGCACTCTTGAATGAAGTTAAGAAGTTAGTATTGGAAAACAATTTGTTTGATCGAGTAATTCAGGTGGAAGTGGGTCAATCCAAGAGTGTAATCCAAATTCAAGAAGGAATTGGAGGGAAGTTAAATATAGATTTAAGTACGCAGTGTAAGGAGGTAAGAGCATCTCGTCTACGAACTCACATTGCTGAGAGGAAGGAGAATATCCTCATTATGCTGGATGATTTATGGAAGGAATATGATCTAGAAAATGATTTTAGAATTTCGTGTCATTCAGAATCAAGGATAGGAGGGTGCAAGATACTTATGACAAGTCGATCTCAACATACATTAAAGAATGAAATGAATACAGAAAAGTTATTTGAAGTGAATTGTCTAACTAAAGAAGAGTCTTGA

Coding sequence (CDS)

ATGGATGCTATAGTTGGAGTATTGGCAGATTATACAATTAAACCTATTTTACATCAATTAGGTTACCTCTTTCTCTACAACATAAATCTTAAGGAAGTTGAAAAGAAACTTGAAACACTTAGGACTACTAAAGATAGCGTGCAAATTTTGGTTAGTCAAGATAAAGGCAAAGGGAATGCAATCTTCGATGAAGTTACCAAGTGGTTGGACAATGTAGAGAATGTGCTTGACATGGCACAACAAAACGAAGGCCCTAATCCCTCGTGCTTTAATTTTGTCAAACGACATCAGTCATCCAGAAAAGCAAAGAAGAGAGTTGAAAATATTATTGAACTCATAAATGAAGAAAGACAATTTAACAAAGATAATGTCGGTTATCCTGTACCTTCTCCAGATACTAATTCTCCTACTCTCCCTGCAGATTACCAAATTATAAAATCAAGAACATCAATGCCTAAGAAAATCAAGGATGCACTTGCAGATCCTAATGTCAACAAGGTTGGAGTATGTGGTATGGGAGGTGTTGGAAAAACTGCACTCTTGAATGAAGTTAAGAAGTTAGTATTGGAAAACAATTTGTTTGATCGAGTAATTCAGGTGGAAGTGGGTCAATCCAAGAGTGTAATCCAAATTCAAGAAGGAATTGGAGGGAAGTTAAATATAGATTTAAGTACGCAGTGTAAGGAGGTAAGAGCATCTCGTCTACGAACTCACATTGCTGAGAGGAAGGAGAATATCCTCATTATGCTGGATGATTTATGGAAGGAATATGATCTAGAAAATGATTTTAGAATTTCGTGTCATTCAGAATCAAGGATAGGAGGGTGCAAGATACTTATGACAAGTCGATCTCAACATACATTAAAGAATGAAATGAATACAGAAAAGTTATTTGAAGTGAATTGTCTAACTAAAGAAGAGTCTTGA

Protein sequence

MDAIVGVLADYTIKPILHQLGYLFLYNINLKEVEKKLETLRTTKDSVQILVSQDKGKGNAIFDEVTKWLDNVENVLDMAQQNEGPNPSCFNFVKRHQSSRKAKKRVENIIELINEERQFNKDNVGYPVPSPDTNSPTLPADYQIIKSRTSMPKKIKDALADPNVNKVGVCGMGGVGKTALLNEVKKLVLENNLFDRVIQVEVGQSKSVIQIQEGIGGKLNIDLSTQCKEVRASRLRTHIAERKENILIMLDDLWKEYDLENDFRISCHSESRIGGCKILMTSRSQHTLKNEMNTEKLFEVNCLTKEES
Homology
BLAST of Lsi10G006260 vs. ExPASy Swiss-Prot
Match: O22727 (Probable disease resistance protein At1g61190 OS=Arabidopsis thaliana OX=3702 GN=At1g61190 PE=3 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 6.5e-19
Identity = 86/299 (28.76%), Postives = 138/299 (46.15%), Query Frame = 0

Query: 21  GYLFLYNINLKEVEKKLETLRTTKDSVQILVSQDKGKGNAIFDEVTKWLDNVENVLDMAQ 80
           GY+     NL+ +++++E LR T+  VQ  V++++ +     + V  WLD V ++    +
Sbjct: 26  GYIRNLEKNLRALQREMEDLRATQHEVQNKVAREESRHQQRLEAVQVWLDRVNSIDIECK 85

Query: 81  QNEGPNP-------SCFNFVKRHQSSRKAKKRVENIIELINE-ERQFNKDNVGYPVPSPD 140
                +P        C    K   SS K  KRV  ++E + + + + N D V  P P  +
Sbjct: 86  DLLSVSPVELQKLCLCGLCSKYVCSSYKYGKRVFLLLEEVTKLKSEGNFDEVSQPPPRSE 145

Query: 141 TNS-PTLPADYQIIKSRTSMPKKIKDALADPNVNKVGVCGMGGVGKTALLNEV-KKLVLE 200
               PT P   Q       M KK  + L +  V  +G+ GMGGVGKT L  ++  K    
Sbjct: 146 VEERPTQPTIGQ-----EEMLKKAWNRLMEDGVGIMGLHGMGGVGKTTLFKKIHNKFAET 205

Query: 201 NNLFDRVIQVEVGQSKSVIQIQEGIGGKLNI-DLSTQCKEVRASRLRTHIAERKENILIM 260
              FD VI + V Q   + ++QE I  KL++ D   + K         H   + +  ++M
Sbjct: 206 GGTFDIVIWIVVSQGAKLSKLQEDIAEKLHLCDDLWKNKNESDKATDIHRVLKGKRFVLM 265

Query: 261 LDDLWKEYDLENDFRISCHSESRIGGCKILMTSRSQHTLKNEMNTEKLFEVNCLTKEES 309
           LDD+W++ DLE    I     S +  CK+  T+R Q     +M   K  +V CL  E++
Sbjct: 266 LDDIWEKVDLE---AIGIPYPSEVNKCKVAFTTRDQKVC-GQMGDHKPMQVKCLEPEDA 315

BLAST of Lsi10G006260 vs. ExPASy Swiss-Prot
Match: O64789 (Probable disease resistance protein At1g61310 OS=Arabidopsis thaliana OX=3702 GN=At1g61310 PE=2 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 2.5e-18
Identity = 85/298 (28.52%), Postives = 138/298 (46.31%), Query Frame = 0

Query: 22  YLFLYNINLKEVEKKLETLRTTKDSVQILVSQDKGKGNAIFDEVTKWLDNVENVLDMAQQ 81
           Y+     NL+ +++++E LR T+  VQ  V++++ +     + V  WLD V ++    + 
Sbjct: 28  YIRTLEKNLRALQREMEDLRATQHEVQNKVAREESRHQQRLEAVQVWLDRVNSIDIECKD 87

Query: 82  NEGPNP-------SCFNFVKRHQSSRKAKKRVENIIELIN-EERQFNKDNVGYPVPSPDT 141
               +P        C    K   SS K  K+V  ++E +   + + N D V  P P  + 
Sbjct: 88  LLSVSPVELQKLCLCGLCTKYVCSSYKYGKKVFLLLEEVKILKSEGNFDEVSQPPPRSEV 147

Query: 142 NS-PTLPADYQIIKSRTSMPKKIKDALADPNVNKVGVCGMGGVGKTALLNEVKKLVLE-N 201
              PT P   Q       M +K  + L +  V  +G+ GMGGVGKT L  ++     E  
Sbjct: 148 EERPTQPTIGQ-----EEMLEKAWNRLMEDGVGIMGLHGMGGVGKTTLFKKIHNKFAEIG 207

Query: 202 NLFDRVIQVEVGQSKSVIQIQEGIGGKLNI-DLSTQCKEVRASRLRTHIAERKENILIML 261
             FD VI + V Q   + ++QE I  KL++ D   + K         H   + +  ++ML
Sbjct: 208 GTFDIVIWIVVSQGAKLSKLQEDIAEKLHLCDDLWKNKNESDKATDIHRVLKGKRFVLML 267

Query: 262 DDLWKEYDLENDFRISCHSESRIGGCKILMTSRSQHTLKNEMNTEKLFEVNCLTKEES 309
           DD+W++ DLE    I     S +  CK+  T+RS+     EM   K  +VNCL  E++
Sbjct: 268 DDIWEKVDLE---AIGIPYPSEVNKCKVAFTTRSREVC-GEMGDHKPMQVNCLEPEDA 316

BLAST of Lsi10G006260 vs. ExPASy Swiss-Prot
Match: Q940K0 (Disease resistance protein UNI OS=Arabidopsis thaliana OX=3702 GN=UNI PE=1 SV=2)

HSP 1 Score: 94.4 bits (233), Expect = 2.5e-18
Identity = 89/315 (28.25%), Postives = 144/315 (45.71%), Query Frame = 0

Query: 8   LADYTIKPILHQL---GYLFLYNINLKEVEKKLETLRTTKDSVQILVSQDKGKGNAIFDE 67
           ++D T+  I + L    Y+     NL+ +++++E LR  +  VQ  V++D+ +     + 
Sbjct: 9   VSDQTLNRIFNCLIGKSYIRTLEKNLRALQREMEDLRAIQHEVQNKVARDEARHQRRLEA 68

Query: 68  VTKWLDNVENVLDMAQQNEGPNP-------SCFNFVKRHQSSRKAKKRVENIIELINE-E 127
           V  WLD V +V    +      P        C    K   SS K  K+V  ++E + +  
Sbjct: 69  VQVWLDRVNSVDIECKDLLSVTPVELQKLCLCGLCSKYVCSSYKYGKKVFLLLEEVKKLN 128

Query: 128 RQFNKDNVGYPVPSPDTNS-PTLPADYQIIKSRTSMPKKIKDALADPNVNKVGVCGMGGV 187
            + N D V  P P  +    PT P   Q       M +K  + L +  V  +G+ GMGGV
Sbjct: 129 SEGNFDEVSQPPPRSEVEERPTQPTIGQ-----EDMLEKAWNRLMEDGVGIMGLHGMGGV 188

Query: 188 GKTALLNEVKKLVLE-NNLFDRVIQVEVGQSKSVIQIQEGIGGKLNI-DLSTQCKEVRAS 247
           GKT L  ++     E    FD VI + V +   + ++QE I  KL++ D   + K     
Sbjct: 189 GKTTLFKKIHNKFAEIGGTFDIVIWIVVSKGVMISKLQEDIAEKLHLCDDLWKNKNESDK 248

Query: 248 RLRTHIAERKENILIMLDDLWKEYDLENDFRISCHSESRIGGCKILMTSRSQHTLKNEMN 307
               H   + +  ++MLDD+W++ DLE    I     S +  CK+  T+RS+     EM 
Sbjct: 249 ATDIHRVLKGKRFVLMLDDIWEKVDLE---AIGIPYPSEVNKCKVAFTTRSREVC-GEMG 308

Query: 308 TEKLFEVNCLTKEES 309
             K  +VNCL  E++
Sbjct: 309 DHKPMQVNCLEPEDA 314

BLAST of Lsi10G006260 vs. ExPASy Swiss-Prot
Match: O82484 (Putative disease resistance protein At4g10780 OS=Arabidopsis thaliana OX=3702 GN=At4g10780 PE=3 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 2.3e-16
Identity = 84/297 (28.28%), Postives = 134/297 (45.12%), Query Frame = 0

Query: 22  YLFLYNINLKEVEKKLETLRTTKDSVQILVSQDKGKGNAIFDEVTKWLDNVENVLD---- 81
           Y+     N+  +EK +E L  T+D V   V  ++GKG     +V  WL  VE + +    
Sbjct: 28  YIHKLKDNIVALEKAIEDLTATRDDVLRRVQMEEGKGLERLQQVQVWLKRVEIIRNQFYD 87

Query: 82  -MAQQNEGPNPSCF--NFVKRHQSSRKAKKRVENIIELINEERQFNKDNVGYPVPSPDTN 141
            ++ +N      CF  N      SS    +RV     +I E    N +     V +P   
Sbjct: 88  LLSARNIEIQRLCFYSNCSTNLSSSYTYGQRV---FLMIKEVENLNSNGFFEIVAAPAPK 147

Query: 142 SPTLPADYQIIKSRTSMPKKIKDALADPNVNKVGVCGMGGVGKTALLNEVKKLVLE-NNL 201
               P     I  R ++ ++  + L D  V  +G+ GMGGVGKT LL ++   + +  N 
Sbjct: 148 LEMRPIQ-PTIMGRETIFQRAWNRLMDDGVGTMGLYGMGGVGKTTLLTQIHNTLHDTKNG 207

Query: 202 FDRVIQVEVGQSKSVIQIQEGIGGKLNIDLSTQCKEVRASRLRTHIAE--RKENILIMLD 261
            D VI V V     + +IQE IG KL   +  +  + + S+    I     K+  +++LD
Sbjct: 208 VDIVIWVVVSSDLQIHKIQEDIGEKLGF-IGKEWNKKQESQKAVDILNCLSKKRFVLLLD 267

Query: 262 DLWKEYDLENDFRISCHSESRIGGCKILMTSRSQHTLKNEMNTEKLFEVNCLTKEES 309
           D+WK+ DL    +I   S++R   CK++ T+RS       M      EV CL+  ++
Sbjct: 268 DIWKKVDLT---KIGIPSQTRENKCKVVFTTRSLDVCA-RMGVHDPMEVQCLSTNDA 315

BLAST of Lsi10G006260 vs. ExPASy Swiss-Prot
Match: Q9FG91 (Probable disease resistance protein At5g43730 OS=Arabidopsis thaliana OX=3702 GN=At5g43730 PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 8.8e-16
Identity = 83/306 (27.12%), Postives = 138/306 (45.10%), Query Frame = 0

Query: 17  LHQLGYLFLYNINLKEVEKKLETLRTTKDSVQILVSQDKGKGNAIFDEVTKWLDNVENV- 76
           L    Y+ L   NL  ++K +E L+  +D +   VS ++ KG      V  WL  V+ V 
Sbjct: 22  LSDSNYIHLMESNLDALQKTMEELKNGRDDLLARVSIEEDKGLQRLALVNGWLSRVQIVE 81

Query: 77  -----LDMAQQNEGPNPSCFNF-----VKRHQSSRKAKKRVENIIELINEERQFNKDNVG 136
                L  A   E      F +     +  +    K  K +E + EL++++         
Sbjct: 82  SEFKDLLEAMSIETGRLCLFGYCSEDCISSYNYGGKVMKNLEEVKELLSKK--------N 141

Query: 137 YPVPSPDTNSPTLPADYQIIKSRTSMPKKIKDALADPNVNKVGVCGMGGVGKTALLNEV- 196
           + V +            Q      +M     ++L D  +  +G+ GMGG+GKT LL  + 
Sbjct: 142 FEVVAQKIIPKAEKKHIQTTVGLDTMVGIAWESLIDDEIRTLGLYGMGGIGKTTLLESLN 201

Query: 197 KKLVLENNLFDRVIQVEVGQSKSVIQIQEGIGGKLNID--LSTQCKEVRASRLRTHIAER 256
            K V   + FD VI V V +   +  IQ+ I G+L  D     + +  +AS +  ++  +
Sbjct: 202 NKFVELESEFDVVIWVVVSKDFQLEGIQDQILGRLRPDKEWERETESKKASLINNNLKRK 261

Query: 257 KENILIMLDDLWKEYDLENDFRISCHSESRIGGCKILMTSRSQHTLKNEMNTEKLFEVNC 309
           K   +++LDDLW E DL    +I     SR  G KI+ T+RS+   K+ M  +K  +V+C
Sbjct: 262 K--FVLLLDDLWSEVDL---IKIGVPPPSRENGSKIVFTTRSKEVCKH-MKADKQIKVDC 313

BLAST of Lsi10G006260 vs. ExPASy TrEMBL
Match: A0A6J1DLB8 (disease resistance protein RPS5-like OS=Momordica charantia OX=3673 GN=LOC111022189 PE=4 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 8.1e-89
Identity = 180/316 (56.96%), Postives = 234/316 (74.05%), Query Frame = 0

Query: 3   AIVGVLADYTIKPILHQLGYLFLYNINLKEVEKKLETLRTTKDSVQILVSQDKGKGNAIF 62
           +IV  +A+YT+ PI  QLGYLF Y+ N+K+VEK+LE L+TT++ VQILV++ +     I+
Sbjct: 6   SIVAKVAEYTVAPIGRQLGYLFFYHKNIKDVEKQLENLKTTREKVQILVNEARSNAYEIY 65

Query: 63  DEVTKWL--DNVENVLDMAQQN------EGPNPSCFNFVKRHQSSRKAKKRVENIIELIN 122
           DEV+ WL  +    + +M  +N      +G N SC NFV+RH+ SR++KKRV++I+E+I 
Sbjct: 66  DEVSTWLLAEVDVELRNMPLENKGVSNRKGSNTSCLNFVQRHKLSRRSKKRVKDILEIIE 125

Query: 123 EERQFNKDNVGYPVPSPDTNSPTLPADYQIIKSRTSMPKKIKDALADPNVNKVGVCGMGG 182
           + ++F  D VGYP P PDT S TLPADYQI++SRT + +KIKD+LADPNVNKVGVCGMGG
Sbjct: 126 KGKKF--DKVGYPAPLPDTKSSTLPADYQILESRTLLAEKIKDSLADPNVNKVGVCGMGG 185

Query: 183 VGKTALLNEVKKLVLENNLFDRVIQVEVGQSKSVIQIQEGIGGKLNIDLSTQC--KEVRA 242
           VGKT LL+EVKKLVLEN LFDRVI V +GQS  V++IQ+ IGGKLN+ L+ +   KE RA
Sbjct: 186 VGKTKLLDEVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAGTKEGRA 245

Query: 243 SRLRTHIAERKENILIMLDDLWKEYDLENDFRISCHSESRIGGCKILMTSRSQHTLKNEM 302
             LR  + E K++ILIMLDDLW EYDL+ +  I C S S   GCKILMTSRS+  L N M
Sbjct: 246 PFLRNKLVEMKDDILIMLDDLWDEYDLQKEVGIPCRSRSSKEGCKILMTSRSRDILTNRM 305

Query: 303 NTEKLFEVNCLTKEES 309
           + EK FEVN L++EES
Sbjct: 306 DAEKCFEVNSLSEEES 319

BLAST of Lsi10G006260 vs. ExPASy TrEMBL
Match: A0A0A0LT62 (SAC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G201260 PE=4 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 3.4e-87
Identity = 168/224 (75.00%), Postives = 195/224 (87.05%), Query Frame = 0

Query: 52  SQDKGKGNAIFDEVTKWLDNVENVLDMAQQNEGPNPSCFNFVKRHQSSRKAKKRVENIIE 111
           S+ K KGNA+FD V+KWL  V++VL++AQQNE  NPSCFNFV+R+Q SRKAKKRVENIIE
Sbjct: 131 SRIKAKGNAVFDGVSKWLTIVKDVLEIAQQNE--NPSCFNFVERYQLSRKAKKRVENIIE 190

Query: 112 LINEERQFNKDNVGYPVPSPDTNSPTLPADYQIIKSRTSMPKKIKDALADPNVNKVGVCG 171
           LINE   FNKDNVGYPVPSPDTNSPTLP DYQII SRTS+ ++IK+ALA+PNV+ VGVCG
Sbjct: 191 LINEGNGFNKDNVGYPVPSPDTNSPTLPTDYQIIASRTSIVEEIKEALANPNVDTVGVCG 250

Query: 172 MGGVGKTALLNEVKKLVLENNLFDRVIQVEVGQSKSVIQIQEGIGGKLNIDLSTQCKEVR 231
           MGGVGKTALLNEVKKLVLE NLFDRVIQVEVG+SKSV  IQE I  +LN++L+ +C+EVR
Sbjct: 251 MGGVGKTALLNEVKKLVLEKNLFDRVIQVEVGESKSVFNIQEQIKDELNMELNIECEEVR 310

Query: 232 ASRLRTHIAERKENILIMLDDLWKEYDLENDFRISCHSESRIGG 276
           A RLRTHIAERKEN+L MLDD+WKE+D+E +F I CHSESR  G
Sbjct: 311 ACRLRTHIAERKENMLFMLDDIWKEHDVEKEFGIPCHSESRKEG 352

BLAST of Lsi10G006260 vs. ExPASy TrEMBL
Match: A0A6J1CPP4 (probable disease resistance protein At4g27220 OS=Momordica charantia OX=3673 GN=LOC111013433 PE=4 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 4.9e-86
Identity = 175/321 (54.52%), Postives = 227/321 (70.72%), Query Frame = 0

Query: 1   MDAIVGV---LADYTIKPILHQLGYLFLYNINLKEVEKKLETLRTTKDSVQILVSQDKGK 60
           MD  + V   +  YT+ PI  QL YLF YN N+K++EK+LE L+ T++ VQ+LV++ +  
Sbjct: 1   MDVFISVGAKVVQYTVAPIGRQLAYLFFYNRNIKDLEKQLENLKATRERVQMLVNEARSN 60

Query: 61  GNAIFDEVTKW--------LDNVENVLDMAQQNEGPNPSCFNFVKRHQSSRKAKKRVENI 120
              I+DEV+ W        L N+    +        N SC NFV+RH+ SRK+KKRV++I
Sbjct: 61  AYEIYDEVSTWLLVEVDVELPNMPRQKEDGSHRNSSNLSCLNFVQRHKLSRKSKKRVKDI 120

Query: 121 IELINEERQFNKDNVGYPVPSPDTNSPTLPADYQIIKSRTSMPKKIKDALADPNVNKVGV 180
           ++LI E ++F K  VG+P P PDT S TLPADYQ+++SRT M +KIKDAL++PNVNKVGV
Sbjct: 121 LQLIEEGKKFEK--VGHPAPLPDTKSSTLPADYQVLESRTLMAEKIKDALSNPNVNKVGV 180

Query: 181 CGMGGVGKTALLNEVKKLVLENNLFDRVIQVEVGQSKSVIQIQEGIGGKLNIDLS--TQC 240
           CGM GVGKT LL+E+KKLVLEN LFDRVI V VG+S  V++IQ+ IGGKLN+ LS   + 
Sbjct: 181 CGMAGVGKTKLLDEIKKLVLENKLFDRVIDVTVGRSNGVVEIQDQIGGKLNMGLSMKAET 240

Query: 241 KEVRASRLRTHIAERKENILIMLDDLWKEYDLENDFRISCHSESRIGGCKILMTSRSQHT 300
           KE RA  LR  + E K+ ILIMLDDLW EYDLE +  I C SES   GCKILMTSRS+  
Sbjct: 241 KEGRAPFLRNKLVEMKDKILIMLDDLWNEYDLEKEVGIPCRSESSKEGCKILMTSRSRDI 300

Query: 301 LKNEMNTEKLFEVNCLTKEES 309
           L N+MNT+K F+VN L+++ES
Sbjct: 301 LTNKMNTKKCFQVNSLSEDES 319

BLAST of Lsi10G006260 vs. ExPASy TrEMBL
Match: A0A5A7TP99 (Disease resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold75G001320 PE=4 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 8.1e-73
Identity = 164/310 (52.90%), Postives = 212/310 (68.39%), Query Frame = 0

Query: 1   MDAIVGVLADYTIKPILHQLGYLFLYNINLKEVEKKLETLRTTKDSVQILVSQDKGKGNA 60
           MD +V V+A  TIKPI HQLGYL  YN N KE+  +LE L TTK  V   V + KGK   
Sbjct: 1   MDILVSVIA-ATIKPIGHQLGYLVCYNRNKKELRDQLENLETTKRDVNQRVQEAKGKSYT 60

Query: 61  IFDEVTKWLDNVENVLDMAQQNEGPNPSCFNFVKRHQSSRKAKKRVENIIELINEERQFN 120
           I +EV+KWL +V+N + +  +    NPSCFN  +R+Q SRK +K+V  I++L+N+   F 
Sbjct: 61  ISEEVSKWLADVDNAI-LHDELSNSNPSCFNLARRYQLSRKREKQVNYILQLMNKRNSFV 120

Query: 121 KDNVGYPVPSPDTNSPTLPADYQIIKSRTSMPKKIKDALADPNVNKVGVCGMGGVGKTAL 180
           +  VGY  P PDT +  +P DYQ+++S+TS+ K IK+ALA P V KVGV GM GVGKT L
Sbjct: 121 E--VGYRAPLPDTENIVVPGDYQVLESKTSLAKDIKNALAKPEVKKVGVYGMAGVGKTYL 180

Query: 181 LNEVKKLVL--ENNLFDRVIQVEVGQSKSVIQIQEGIGGKLNIDLSTQCKEVRASRLRTH 240
           LNEVKKLVL  E+ LFDRVI V VG+   V +IQE IG +LNI+L  + KE RAS LR +
Sbjct: 181 LNEVKKLVLKGEDRLFDRVIDVRVGRFNDVTEIQEQIGDQLNIEL-PKSKEGRASFLRNN 240

Query: 241 IAERKENILIMLDDLWKEYDLENDFRISCHSESRIGGCKILMTSRSQHTLKNEMNTEKLF 300
           + + + NILI+LDDLWKEYDL  +  I    E    GCK+LMTSRSQ  L N MNT++ F
Sbjct: 241 LVKMEGNILILLDDLWKEYDLLKEIGIPLSKE----GCKVLMTSRSQDILTNNMNTQECF 300

Query: 301 EVNCLTKEES 309
           +V+ L++EES
Sbjct: 301 QVSSLSEEES 301

BLAST of Lsi10G006260 vs. ExPASy TrEMBL
Match: A0A5D3BQI3 (Disease resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold38G00050 PE=4 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 2.4e-72
Identity = 163/310 (52.58%), Postives = 211/310 (68.06%), Query Frame = 0

Query: 1   MDAIVGVLADYTIKPILHQLGYLFLYNINLKEVEKKLETLRTTKDSVQILVSQDKGKGNA 60
           MD +V V+A  TIKPI HQLGYL  YN N KE+  +LE L TTK  V   V + KGK   
Sbjct: 1   MDILVSVIA-ATIKPIGHQLGYLVCYNRNKKELRDQLENLETTKRDVNQRVQEAKGKSYT 60

Query: 61  IFDEVTKWLDNVENVLDMAQQNEGPNPSCFNFVKRHQSSRKAKKRVENIIELINEERQFN 120
           I +EV+KWL +V+N + +  +    NPSCFN  +R+Q SRK +K+V  I++L+N+   F 
Sbjct: 61  ISEEVSKWLADVDNAI-LHDELSNSNPSCFNLARRYQLSRKREKQVNYILQLMNKRNSFV 120

Query: 121 KDNVGYPVPSPDTNSPTLPADYQIIKSRTSMPKKIKDALADPNVNKVGVCGMGGVGKTAL 180
           +  VGY  P PDT +  +P DYQ+++S+TS+ K IK+ALA P V KVGV GM GVGKT L
Sbjct: 121 E--VGYRAPLPDTENIVVPGDYQVLESKTSLAKDIKNALAKPEVKKVGVYGMAGVGKTYL 180

Query: 181 LNEVKKLVL--ENNLFDRVIQVEVGQSKSVIQIQEGIGGKLNIDLSTQCKEVRASRLRTH 240
           LNEVKKLVL  E+ LFDR I V VG+   V +IQE IG +LNI+L  + KE RAS LR +
Sbjct: 181 LNEVKKLVLKGEDRLFDRAIDVRVGRFNDVTEIQEQIGDQLNIEL-PKSKEGRASFLRNN 240

Query: 241 IAERKENILIMLDDLWKEYDLENDFRISCHSESRIGGCKILMTSRSQHTLKNEMNTEKLF 300
           + + + NILI+LDDLWKEYDL  +  I    E    GCK+LMTSRSQ  L N MNT++ F
Sbjct: 241 LVKMEGNILILLDDLWKEYDLLKEIGIPLSKE----GCKVLMTSRSQDILTNNMNTQECF 300

Query: 301 EVNCLTKEES 309
           +V+ L++EES
Sbjct: 301 QVSSLSEEES 301

BLAST of Lsi10G006260 vs. NCBI nr
Match: XP_038887230.1 (probable disease resistance protein At5g63020 [Benincasa hispida])

HSP 1 Score: 462.6 bits (1189), Expect = 2.6e-126
Identity = 241/309 (77.99%), Postives = 266/309 (86.08%), Query Frame = 0

Query: 1   MDAIVGVLADYTIKPILHQLGYLFLYNINLKEVEKKLETLRTTKDSVQILVSQDKGKGNA 60
           MDA+VGVLADYTIKPILHQL YLF Y  N+KEVEKKL  LRTT+ +VQILV+QDKGKGNA
Sbjct: 73  MDAVVGVLADYTIKPILHQLDYLFHYKGNIKEVEKKLGILRTTERTVQILVNQDKGKGNA 132

Query: 61  IFDEVTKWLDNVENVLDMAQQNEGPNPSCFNFVKRHQSSRKAKKRVENIIELINEERQFN 120
           IFD VT WLDNV+NVLDMAQQNE PNPSCFN V+RHQ SRKAKKR+E+I ELINE RQFN
Sbjct: 133 IFDGVTNWLDNVKNVLDMAQQNEAPNPSCFNCVQRHQLSRKAKKRLEDINELINEGRQFN 192

Query: 121 KDNVGYPVPSPDTNSPTLPADYQIIKSRTSMPKKIKDALADPNVNKVGVCGMGGVGKTAL 180
           KDNVGYPVPSPDT+S TLPADYQ  +SRTSM +KIKDALA+PNVNKVGVCGMGGVGKT L
Sbjct: 193 KDNVGYPVPSPDTSSSTLPADYQFFESRTSMAEKIKDALANPNVNKVGVCGMGGVGKTTL 252

Query: 181 LNEVKKLVLENNLFDRVIQVEVGQSKSVIQIQEGIGGKLNID-LSTQCKEVRASRLRTHI 240
           L EVKKLVLEN LFDRVIQVEVGQSKSVI IQE I  KLN++ L+ + +EVR SRL+THI
Sbjct: 253 LKEVKKLVLENKLFDRVIQVEVGQSKSVIHIQEEIRAKLNMEYLNMEFEEVRTSRLQTHI 312

Query: 241 AERKENILIMLDDLWKEYDLENDFRISCHSESRIGGCKILMTSRSQHTLKNEMNTEKLFE 300
           AE+KENIL MLDDLWKEYD+E +F I   S+SRI GCKILMTSR +    N+MN EKLFE
Sbjct: 313 AEKKENILFMLDDLWKEYDVEKEFGIPYRSDSRIEGCKILMTSRRRDLFTNQMNIEKLFE 372

Query: 301 VNCLTKEES 309
           VN LTKEES
Sbjct: 373 VNSLTKEES 381

BLAST of Lsi10G006260 vs. NCBI nr
Match: XP_038887227.1 (disease resistance protein At4g27190-like [Benincasa hispida])

HSP 1 Score: 450.3 bits (1157), Expect = 1.4e-122
Identity = 228/308 (74.03%), Postives = 265/308 (86.04%), Query Frame = 0

Query: 1   MDAIVGVLADYTIKPILHQLGYLFLYNINLKEVEKKLETLRTTKDSVQILVSQDKGKGNA 60
           MDA+VGV+ +YTI+PILHQLGYLF YN N+K++++K +TL TT+D+VQILVSQDKGKGNA
Sbjct: 1   MDAVVGVVVEYTIRPILHQLGYLFRYNGNVKKLQEKHKTLETTRDTVQILVSQDKGKGNA 60

Query: 61  IFDEVTKWLDNVENVLDMAQQNEGPNPSCFNFVKRHQSSRKAKKRVENIIELINEERQFN 120
           IFDEVTKWLDNV+ V +M +QN+  NPSCFNFVKRH+ SRK+KK VE+I ELINE R+FN
Sbjct: 61  IFDEVTKWLDNVKEVSNMTKQNKELNPSCFNFVKRHRLSRKSKKMVEDIAELINEGRRFN 120

Query: 121 KDNVGYPVPSPDTNSPTLPADYQIIKSRTSMPKKIKDALADPNVNKVGVCGMGGVGKTAL 180
           KD +GYPVPS DTN PT+P DYQII+SRT + + IKDALA+PNVNKVGVCGMGGVGKT+L
Sbjct: 121 KDRIGYPVPSSDTNCPTIPTDYQIIESRTLLVENIKDALANPNVNKVGVCGMGGVGKTSL 180

Query: 181 LNEVKKLVLENNLFDRVIQVEVGQSKSVIQIQEGIGGKLNIDLSTQCKEVRASRLRTHIA 240
           LNEVKKLVLENNLFDRVIQVEVGQSKSVI IQE I G LN+    QC ++ AS L+THI 
Sbjct: 181 LNEVKKLVLENNLFDRVIQVEVGQSKSVIDIQEEIRGALNM----QCGKITASLLQTHII 240

Query: 241 ERKENILIMLDDLWKEYDLENDFRISCHSESRIGGCKILMTSRSQHTLKNEMNTEKLFEV 300
           ERKENIL MLDDLWK+YDLE +F I C SESR  GCKILMTSRS+HTL N+MN EKLFEV
Sbjct: 241 ERKENILFMLDDLWKDYDLEKEFGIPCRSESRTEGCKILMTSRSRHTLTNQMNIEKLFEV 300

Query: 301 NCLTKEES 309
           N +TK+ES
Sbjct: 301 NPVTKKES 304

BLAST of Lsi10G006260 vs. NCBI nr
Match: XP_038887228.1 (probable disease resistance protein At5g43740 [Benincasa hispida])

HSP 1 Score: 446.0 bits (1146), Expect = 2.6e-121
Identity = 227/308 (73.70%), Postives = 260/308 (84.42%), Query Frame = 0

Query: 1   MDAIVGVLADYTIKPILHQLGYLFLYNINLKEVEKKLETLRTTKDSVQILVSQDKGKGNA 60
           MDA+VGV+ +YTI PILHQLGYLF YN N+K++++K  TL TTKD+VQILVSQDKGKGNA
Sbjct: 1   MDAVVGVVVEYTIGPILHQLGYLFRYNGNIKKLQEKHRTLETTKDTVQILVSQDKGKGNA 60

Query: 61  IFDEVTKWLDNVENVLDMAQQNEGPNPSCFNFVKRHQSSRKAKKRVENIIELINEERQFN 120
           IF+EVTKWLDNV+ V DM +QNE  NPSCFNFVKRHQ SRKAKK VE+I ELINE RQFN
Sbjct: 61  IFNEVTKWLDNVKEVSDMTKQNEELNPSCFNFVKRHQLSRKAKKMVEDIAELINEGRQFN 120

Query: 121 KDNVGYPVPSPDTNSPTLPADYQIIKSRTSMPKKIKDALADPNVNKVGVCGMGGVGKTAL 180
           KDN+ YPVPS D N PT+P DYQII+SRT + + IKDALA+PNVNK+GVCGM GVGKT+L
Sbjct: 121 KDNIDYPVPSSDINCPTIPTDYQIIESRTLLVENIKDALANPNVNKLGVCGMAGVGKTSL 180

Query: 181 LNEVKKLVLENNLFDRVIQVEVGQSKSVIQIQEGIGGKLNIDLSTQCKEVRASRLRTHIA 240
           LNEVKKLVLE+NLFDRVIQVEVGQSK+VI IQE I G LN+    QC ++ AS L+THI 
Sbjct: 181 LNEVKKLVLESNLFDRVIQVEVGQSKNVIDIQEEIRGALNM----QCGKITASLLQTHIT 240

Query: 241 ERKENILIMLDDLWKEYDLENDFRISCHSESRIGGCKILMTSRSQHTLKNEMNTEKLFEV 300
           ERKENIL M DDLWK+YDLE +F I C SESR  GCKILMTSRS+HTL N+MN EKLFE+
Sbjct: 241 ERKENILFMFDDLWKDYDLEKEFGIPCRSESRTEGCKILMTSRSRHTLTNQMNIEKLFEL 300

Query: 301 NCLTKEES 309
           N LTK+ES
Sbjct: 301 NSLTKKES 304

BLAST of Lsi10G006260 vs. NCBI nr
Match: XP_022155050.1 (disease resistance protein RPS5-like [Momordica charantia])

HSP 1 Score: 337.0 bits (863), Expect = 1.7e-88
Identity = 180/316 (56.96%), Postives = 234/316 (74.05%), Query Frame = 0

Query: 3   AIVGVLADYTIKPILHQLGYLFLYNINLKEVEKKLETLRTTKDSVQILVSQDKGKGNAIF 62
           +IV  +A+YT+ PI  QLGYLF Y+ N+K+VEK+LE L+TT++ VQILV++ +     I+
Sbjct: 6   SIVAKVAEYTVAPIGRQLGYLFFYHKNIKDVEKQLENLKTTREKVQILVNEARSNAYEIY 65

Query: 63  DEVTKWL--DNVENVLDMAQQN------EGPNPSCFNFVKRHQSSRKAKKRVENIIELIN 122
           DEV+ WL  +    + +M  +N      +G N SC NFV+RH+ SR++KKRV++I+E+I 
Sbjct: 66  DEVSTWLLAEVDVELRNMPLENKGVSNRKGSNTSCLNFVQRHKLSRRSKKRVKDILEIIE 125

Query: 123 EERQFNKDNVGYPVPSPDTNSPTLPADYQIIKSRTSMPKKIKDALADPNVNKVGVCGMGG 182
           + ++F  D VGYP P PDT S TLPADYQI++SRT + +KIKD+LADPNVNKVGVCGMGG
Sbjct: 126 KGKKF--DKVGYPAPLPDTKSSTLPADYQILESRTLLAEKIKDSLADPNVNKVGVCGMGG 185

Query: 183 VGKTALLNEVKKLVLENNLFDRVIQVEVGQSKSVIQIQEGIGGKLNIDLSTQC--KEVRA 242
           VGKT LL+EVKKLVLEN LFDRVI V +GQS  V++IQ+ IGGKLN+ L+ +   KE RA
Sbjct: 186 VGKTKLLDEVKKLVLENKLFDRVIDVTIGQSNGVVEIQDQIGGKLNMGLNMKAGTKEGRA 245

Query: 243 SRLRTHIAERKENILIMLDDLWKEYDLENDFRISCHSESRIGGCKILMTSRSQHTLKNEM 302
             LR  + E K++ILIMLDDLW EYDL+ +  I C S S   GCKILMTSRS+  L N M
Sbjct: 246 PFLRNKLVEMKDDILIMLDDLWDEYDLQKEVGIPCRSRSSKEGCKILMTSRSRDILTNRM 305

Query: 303 NTEKLFEVNCLTKEES 309
           + EK FEVN L++EES
Sbjct: 306 DAEKCFEVNSLSEEES 319

BLAST of Lsi10G006260 vs. NCBI nr
Match: XP_022143564.1 (probable disease resistance protein At4g27220 [Momordica charantia])

HSP 1 Score: 327.8 bits (839), Expect = 1.0e-85
Identity = 175/321 (54.52%), Postives = 227/321 (70.72%), Query Frame = 0

Query: 1   MDAIVGV---LADYTIKPILHQLGYLFLYNINLKEVEKKLETLRTTKDSVQILVSQDKGK 60
           MD  + V   +  YT+ PI  QL YLF YN N+K++EK+LE L+ T++ VQ+LV++ +  
Sbjct: 1   MDVFISVGAKVVQYTVAPIGRQLAYLFFYNRNIKDLEKQLENLKATRERVQMLVNEARSN 60

Query: 61  GNAIFDEVTKW--------LDNVENVLDMAQQNEGPNPSCFNFVKRHQSSRKAKKRVENI 120
              I+DEV+ W        L N+    +        N SC NFV+RH+ SRK+KKRV++I
Sbjct: 61  AYEIYDEVSTWLLVEVDVELPNMPRQKEDGSHRNSSNLSCLNFVQRHKLSRKSKKRVKDI 120

Query: 121 IELINEERQFNKDNVGYPVPSPDTNSPTLPADYQIIKSRTSMPKKIKDALADPNVNKVGV 180
           ++LI E ++F K  VG+P P PDT S TLPADYQ+++SRT M +KIKDAL++PNVNKVGV
Sbjct: 121 LQLIEEGKKFEK--VGHPAPLPDTKSSTLPADYQVLESRTLMAEKIKDALSNPNVNKVGV 180

Query: 181 CGMGGVGKTALLNEVKKLVLENNLFDRVIQVEVGQSKSVIQIQEGIGGKLNIDLS--TQC 240
           CGM GVGKT LL+E+KKLVLEN LFDRVI V VG+S  V++IQ+ IGGKLN+ LS   + 
Sbjct: 181 CGMAGVGKTKLLDEIKKLVLENKLFDRVIDVTVGRSNGVVEIQDQIGGKLNMGLSMKAET 240

Query: 241 KEVRASRLRTHIAERKENILIMLDDLWKEYDLENDFRISCHSESRIGGCKILMTSRSQHT 300
           KE RA  LR  + E K+ ILIMLDDLW EYDLE +  I C SES   GCKILMTSRS+  
Sbjct: 241 KEGRAPFLRNKLVEMKDKILIMLDDLWNEYDLEKEVGIPCRSESSKEGCKILMTSRSRDI 300

Query: 301 LKNEMNTEKLFEVNCLTKEES 309
           L N+MNT+K F+VN L+++ES
Sbjct: 301 LTNKMNTKKCFQVNSLSEDES 319

BLAST of Lsi10G006260 vs. TAIR 10
Match: AT1G61190.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 96.3 bits (238), Expect = 4.6e-20
Identity = 86/299 (28.76%), Postives = 138/299 (46.15%), Query Frame = 0

Query: 21  GYLFLYNINLKEVEKKLETLRTTKDSVQILVSQDKGKGNAIFDEVTKWLDNVENVLDMAQ 80
           GY+     NL+ +++++E LR T+  VQ  V++++ +     + V  WLD V ++    +
Sbjct: 26  GYIRNLEKNLRALQREMEDLRATQHEVQNKVAREESRHQQRLEAVQVWLDRVNSIDIECK 85

Query: 81  QNEGPNP-------SCFNFVKRHQSSRKAKKRVENIIELINE-ERQFNKDNVGYPVPSPD 140
                +P        C    K   SS K  KRV  ++E + + + + N D V  P P  +
Sbjct: 86  DLLSVSPVELQKLCLCGLCSKYVCSSYKYGKRVFLLLEEVTKLKSEGNFDEVSQPPPRSE 145

Query: 141 TNS-PTLPADYQIIKSRTSMPKKIKDALADPNVNKVGVCGMGGVGKTALLNEV-KKLVLE 200
               PT P   Q       M KK  + L +  V  +G+ GMGGVGKT L  ++  K    
Sbjct: 146 VEERPTQPTIGQ-----EEMLKKAWNRLMEDGVGIMGLHGMGGVGKTTLFKKIHNKFAET 205

Query: 201 NNLFDRVIQVEVGQSKSVIQIQEGIGGKLNI-DLSTQCKEVRASRLRTHIAERKENILIM 260
              FD VI + V Q   + ++QE I  KL++ D   + K         H   + +  ++M
Sbjct: 206 GGTFDIVIWIVVSQGAKLSKLQEDIAEKLHLCDDLWKNKNESDKATDIHRVLKGKRFVLM 265

Query: 261 LDDLWKEYDLENDFRISCHSESRIGGCKILMTSRSQHTLKNEMNTEKLFEVNCLTKEES 309
           LDD+W++ DLE    I     S +  CK+  T+R Q     +M   K  +V CL  E++
Sbjct: 266 LDDIWEKVDLE---AIGIPYPSEVNKCKVAFTTRDQKVC-GQMGDHKPMQVKCLEPEDA 315

BLAST of Lsi10G006260 vs. TAIR 10
Match: AT1G61180.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 94.4 bits (233), Expect = 1.8e-19
Identity = 89/315 (28.25%), Postives = 144/315 (45.71%), Query Frame = 0

Query: 8   LADYTIKPILHQL---GYLFLYNINLKEVEKKLETLRTTKDSVQILVSQDKGKGNAIFDE 67
           ++D T+  I + L    Y+     NL+ +++++E LR  +  VQ  V++D+ +     + 
Sbjct: 9   VSDQTLNRIFNCLIGKSYIRTLEKNLRALQREMEDLRAIQHEVQNKVARDEARHQRRLEA 68

Query: 68  VTKWLDNVENVLDMAQQNEGPNP-------SCFNFVKRHQSSRKAKKRVENIIELINE-E 127
           V  WLD V +V    +      P        C    K   SS K  K+V  ++E + +  
Sbjct: 69  VQVWLDRVNSVDIECKDLLSVTPVELQKLCLCGLCSKYVCSSYKYGKKVFLLLEEVKKLN 128

Query: 128 RQFNKDNVGYPVPSPDTNS-PTLPADYQIIKSRTSMPKKIKDALADPNVNKVGVCGMGGV 187
            + N D V  P P  +    PT P   Q       M +K  + L +  V  +G+ GMGGV
Sbjct: 129 SEGNFDEVSQPPPRSEVEERPTQPTIGQ-----EDMLEKAWNRLMEDGVGIMGLHGMGGV 188

Query: 188 GKTALLNEVKKLVLE-NNLFDRVIQVEVGQSKSVIQIQEGIGGKLNI-DLSTQCKEVRAS 247
           GKT L  ++     E    FD VI + V +   + ++QE I  KL++ D   + K     
Sbjct: 189 GKTTLFKKIHNKFAEIGGTFDIVIWIVVSKGVMISKLQEDIAEKLHLCDDLWKNKNESDK 248

Query: 248 RLRTHIAERKENILIMLDDLWKEYDLENDFRISCHSESRIGGCKILMTSRSQHTLKNEMN 307
               H   + +  ++MLDD+W++ DLE    I     S +  CK+  T+RS+     EM 
Sbjct: 249 ATDIHRVLKGKRFVLMLDDIWEKVDLE---AIGIPYPSEVNKCKVAFTTRSREVC-GEMG 308

Query: 308 TEKLFEVNCLTKEES 309
             K  +VNCL  E++
Sbjct: 309 DHKPMQVNCLEPEDA 314

BLAST of Lsi10G006260 vs. TAIR 10
Match: AT1G61310.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 94.4 bits (233), Expect = 1.8e-19
Identity = 85/298 (28.52%), Postives = 138/298 (46.31%), Query Frame = 0

Query: 22  YLFLYNINLKEVEKKLETLRTTKDSVQILVSQDKGKGNAIFDEVTKWLDNVENVLDMAQQ 81
           Y+     NL+ +++++E LR T+  VQ  V++++ +     + V  WLD V ++    + 
Sbjct: 28  YIRTLEQNLRALQREMEDLRATQHEVQNKVAREESRHQQRLEAVQVWLDRVNSIDIECKD 87

Query: 82  NEGPNP-------SCFNFVKRHQSSRKAKKRVENIIELIN-EERQFNKDNVGYPVPSPDT 141
               +P        C    K   SS K  K+V  ++E +   + + N D V  P P  + 
Sbjct: 88  LLSVSPVELQKLCLCGLCTKYVCSSYKYGKKVFLLLEEVKILKSEGNFDEVSQPPPRSEV 147

Query: 142 NS-PTLPADYQIIKSRTSMPKKIKDALADPNVNKVGVCGMGGVGKTALLNEVKKLVLE-N 201
              PT P   Q       M +K  + L +  V  +G+ GMGGVGKT L  ++     E  
Sbjct: 148 EERPTQPTIGQ-----EEMLEKAWNRLMEDGVGIMGLHGMGGVGKTTLFKKIHNKFAEIG 207

Query: 202 NLFDRVIQVEVGQSKSVIQIQEGIGGKLNI-DLSTQCKEVRASRLRTHIAERKENILIML 261
             FD VI + V Q   + ++QE I  KL++ D   + K         H   + +  ++ML
Sbjct: 208 GTFDIVIWIVVSQGAKLSKLQEDIAEKLHLCDDLWKNKNESDKATDIHRVLKGKRFVLML 267

Query: 262 DDLWKEYDLENDFRISCHSESRIGGCKILMTSRSQHTLKNEMNTEKLFEVNCLTKEES 309
           DD+W++ DLE    I     S +  CK+  T+RS+     EM   K  +VNCL  E++
Sbjct: 268 DDIWEKVDLE---AIGIPYPSEVNKCKVAFTTRSREVC-GEMGDHKPMQVNCLEPEDA 316

BLAST of Lsi10G006260 vs. TAIR 10
Match: AT1G61180.2 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 94.4 bits (233), Expect = 1.8e-19
Identity = 89/315 (28.25%), Postives = 144/315 (45.71%), Query Frame = 0

Query: 8   LADYTIKPILHQL---GYLFLYNINLKEVEKKLETLRTTKDSVQILVSQDKGKGNAIFDE 67
           ++D T+  I + L    Y+     NL+ +++++E LR  +  VQ  V++D+ +     + 
Sbjct: 9   VSDQTLNRIFNCLIGKSYIRTLEKNLRALQREMEDLRAIQHEVQNKVARDEARHQRRLEA 68

Query: 68  VTKWLDNVENVLDMAQQNEGPNP-------SCFNFVKRHQSSRKAKKRVENIIELINE-E 127
           V  WLD V +V    +      P        C    K   SS K  K+V  ++E + +  
Sbjct: 69  VQVWLDRVNSVDIECKDLLSVTPVELQKLCLCGLCSKYVCSSYKYGKKVFLLLEEVKKLN 128

Query: 128 RQFNKDNVGYPVPSPDTNS-PTLPADYQIIKSRTSMPKKIKDALADPNVNKVGVCGMGGV 187
            + N D V  P P  +    PT P   Q       M +K  + L +  V  +G+ GMGGV
Sbjct: 129 SEGNFDEVSQPPPRSEVEERPTQPTIGQ-----EDMLEKAWNRLMEDGVGIMGLHGMGGV 188

Query: 188 GKTALLNEVKKLVLE-NNLFDRVIQVEVGQSKSVIQIQEGIGGKLNI-DLSTQCKEVRAS 247
           GKT L  ++     E    FD VI + V +   + ++QE I  KL++ D   + K     
Sbjct: 189 GKTTLFKKIHNKFAEIGGTFDIVIWIVVSKGVMISKLQEDIAEKLHLCDDLWKNKNESDK 248

Query: 248 RLRTHIAERKENILIMLDDLWKEYDLENDFRISCHSESRIGGCKILMTSRSQHTLKNEMN 307
               H   + +  ++MLDD+W++ DLE    I     S +  CK+  T+RS+     EM 
Sbjct: 249 ATDIHRVLKGKRFVLMLDDIWEKVDLE---AIGIPYPSEVNKCKVAFTTRSREVC-GEMG 308

Query: 308 TEKLFEVNCLTKEES 309
             K  +VNCL  E++
Sbjct: 309 DHKPMQVNCLEPEDA 314

BLAST of Lsi10G006260 vs. TAIR 10
Match: AT4G10780.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 87.8 bits (216), Expect = 1.6e-17
Identity = 84/297 (28.28%), Postives = 134/297 (45.12%), Query Frame = 0

Query: 22  YLFLYNINLKEVEKKLETLRTTKDSVQILVSQDKGKGNAIFDEVTKWLDNVENVLD---- 81
           Y+     N+  +EK +E L  T+D V   V  ++GKG     +V  WL  VE + +    
Sbjct: 28  YIHKLKDNIVALEKAIEDLTATRDDVLRRVQMEEGKGLERLQQVQVWLKRVEIIRNQFYD 87

Query: 82  -MAQQNEGPNPSCF--NFVKRHQSSRKAKKRVENIIELINEERQFNKDNVGYPVPSPDTN 141
            ++ +N      CF  N      SS    +RV     +I E    N +     V +P   
Sbjct: 88  LLSARNIEIQRLCFYSNCSTNLSSSYTYGQRV---FLMIKEVENLNSNGFFEIVAAPAPK 147

Query: 142 SPTLPADYQIIKSRTSMPKKIKDALADPNVNKVGVCGMGGVGKTALLNEVKKLVLE-NNL 201
               P     I  R ++ ++  + L D  V  +G+ GMGGVGKT LL ++   + +  N 
Sbjct: 148 LEMRPIQ-PTIMGRETIFQRAWNRLMDDGVGTMGLYGMGGVGKTTLLTQIHNTLHDTKNG 207

Query: 202 FDRVIQVEVGQSKSVIQIQEGIGGKLNIDLSTQCKEVRASRLRTHIAE--RKENILIMLD 261
            D VI V V     + +IQE IG KL   +  +  + + S+    I     K+  +++LD
Sbjct: 208 VDIVIWVVVSSDLQIHKIQEDIGEKLGF-IGKEWNKKQESQKAVDILNCLSKKRFVLLLD 267

Query: 262 DLWKEYDLENDFRISCHSESRIGGCKILMTSRSQHTLKNEMNTEKLFEVNCLTKEES 309
           D+WK+ DL    +I   S++R   CK++ T+RS       M      EV CL+  ++
Sbjct: 268 DIWKKVDLT---KIGIPSQTRENKCKVVFTTRSLDVCA-RMGVHDPMEVQCLSTNDA 315

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O227276.5e-1928.76Probable disease resistance protein At1g61190 OS=Arabidopsis thaliana OX=3702 GN... [more]
O647892.5e-1828.52Probable disease resistance protein At1g61310 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q940K02.5e-1828.25Disease resistance protein UNI OS=Arabidopsis thaliana OX=3702 GN=UNI PE=1 SV=2[more]
O824842.3e-1628.28Putative disease resistance protein At4g10780 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9FG918.8e-1627.12Probable disease resistance protein At5g43730 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1DLB88.1e-8956.96disease resistance protein RPS5-like OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A0A0LT623.4e-8775.00SAC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G201260 PE=4 SV... [more]
A0A6J1CPP44.9e-8654.52probable disease resistance protein At4g27220 OS=Momordica charantia OX=3673 GN=... [more]
A0A5A7TP998.1e-7352.90Disease resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A5D3BQI32.4e-7252.58Disease resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
Match NameE-valueIdentityDescription
XP_038887230.12.6e-12677.99probable disease resistance protein At5g63020 [Benincasa hispida][more]
XP_038887227.11.4e-12274.03disease resistance protein At4g27190-like [Benincasa hispida][more]
XP_038887228.12.6e-12173.70probable disease resistance protein At5g43740 [Benincasa hispida][more]
XP_022155050.11.7e-8856.96disease resistance protein RPS5-like [Momordica charantia][more]
XP_022143564.11.0e-8554.52probable disease resistance protein At4g27220 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G61190.14.6e-2028.76LRR and NB-ARC domains-containing disease resistance protein [more]
AT1G61180.11.8e-1928.25LRR and NB-ARC domains-containing disease resistance protein [more]
AT1G61310.11.8e-1928.52LRR and NB-ARC domains-containing disease resistance protein [more]
AT1G61180.21.8e-1928.25LRR and NB-ARC domains-containing disease resistance protein [more]
AT4G10780.11.6e-1728.28LRR and NB-ARC domains-containing disease resistance protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 30..50
NoneNo IPR availablePRINTSPR00364DISEASERSISTcoord: 166..181
score: 53.44
coord: 240..254
score: 32.83
NoneNo IPR availablePANTHERPTHR33463:SF57PHOSPHATASE, PUTATIVE-RELATEDcoord: 2..307
NoneNo IPR availablePANTHERPTHR33463FAMILY NOT NAMEDcoord: 2..307
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 132..302
e-value: 1.0E-24
score: 89.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 137..308
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 151..308
e-value: 4.1E-24
score: 85.1

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G006260.1Lsi10G006260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0043531 ADP binding