Lsi10G006250 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi10G006250 vs. ExPASy Swiss-Prot
Match: O81825 (Probable disease resistance protein At4g27220 OS=Arabidopsis thaliana OX=3702 GN=At4g27220 PE=2 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 5.7e-34
Identity = 163/637 (25.59%), Postives = 287/637 (45.05%), Query Frame = 0

Query: 5   IQEIAKDVVKECGGLPIALKILAKALKGK-RVEIWKDTCKKLKDPILVNIPGV--SDQLY 64
           ++ IAKDV  EC GLP+A+  + + L+GK +VE+WK T   LK     + P +   ++++
Sbjct: 294 VKPIAKDVSHECCGLPLAIITIGRTLRGKPQVEVWKHTLNLLKR----SAPSIDTEEKIF 353

Query: 65  SCLQFSYDSIEDEAKLILFLCCVFPDDHRINLKDLQMYALSMRLLNGISAWRDARNRVIK 124
             L+ SYD ++D  K     C +FP+D+ I + +L MY ++  LL+G   + D  N  + 
Sbjct: 354 GTLKLSYDFLQDNMKSCFLFCALFPEDYSIKVSELIMYWVAEGLLDGQHHYEDMMNEGVT 413

Query: 125 LVDDLKSSSLLLKSDNGNDCVQMHDVVHDVAKYIASK----IHKMPSLSYGSGESEWRDE 184
           LV+ LK S LL   D+  D V+MHDVV D A +  S      H +  +  G G  E+  +
Sbjct: 414 LVERLKDSCLLEDGDS-CDTVKMHDVVRDFAIWFMSSQGEGFHSL--VMAGRGLIEFPQD 473

Query: 185 DICGSYNAIYINCKKFLNFPPK-LEFPNLQLFILSGVSDFRSAIRIPDTFFEGMKELRVL 244
               S   + +   K    P   +E     + +L G S  +    +P+ F +    LR+L
Sbjct: 474 KFVSSVQRVSLMANKLERLPNNVIEGVETLVLLLQGNSHVK---EVPNGFLQAFPNLRIL 533

Query: 245 D-----VRTMCFAPSSWTSLTQSLKNLQTLCLTMREGSDFGTIGELKKLEILKIISSSFF 304
           D     +RT+   P S+++L  SL++L  +    ++  +  ++  L KL+ L +  S+  
Sbjct: 534 DLSGVRIRTL---PDSFSNL-HSLRSL--VLRNCKKLRNLPSLESLVKLQFLDLHESA-I 593

Query: 305 QVLPASMSELTQLKVLHIL-CPGLKLIHPNILTSMRRLEELIVGCDYKRWGDEVTYKNKL 364
           + LP  +  L+ L+ + +     L+ I    +  +  LE L +      WG +   +   
Sbjct: 594 RELPRGLEALSSLRYICVSNTYQLQSIPAGTILQLSSLEVLDMAGSAYSWGIKGEERE-- 653

Query: 365 IKNVKLSELDCLTRLLFLKLNIQNVDILSNELSLDR-AQSLEEFHIQVGNYVYS-LRSDK 424
                L E+ CL  L FL + +  +D+LS     D   + L +F      +++S +RS  
Sbjct: 654 -GQATLDEVTCLPHLQFLAIKL--LDVLSFSYEFDSLTKRLTKF-----QFLFSPIRSVS 713

Query: 425 FKTSLE-----LETKSPIGSIGGVLQII----LQKC-------ENLVVKDSVDFTKLFCN 484
              + E      +      SIG +LQ +    L  C       ENLV K    F  +   
Sbjct: 714 PPGTGEGCLAISDVNVSNASIGWLLQHVTSLDLNYCEGLNGMFENLVTKSKSSFVAM--K 773

Query: 485 PNGNYGYPRLKHLQIIIREQDEDMPHGIRNITPHFDLQLWHHNNGLTANSFCKLQRICIN 544
               + +P L  L      Q +  P+       + +L+     NG       KL+ + ++
Sbjct: 774 ALSIHYFPSLS-LASGCESQLDLFPNLEELSLDNVNLESIGELNGFLGMRLQKLKLLQVS 833

Query: 545 GCNKLTSVFPSHMVI-SLKFLNTLEVRYCKLVERIFEVEEQSNG--VNQVMPMVST-KLN 604
           GC +L  +F   ++  +L  L  ++V  C  +E +F             ++P ++  KL 
Sbjct: 834 GCRQLKRLFSDQILAGTLPNLQEIKVVSCLRLEELFNFSSVPVDFCAESLLPKLTVIKLK 893

Query: 605 FLPNLKHMWNNDSARELLIFPNLKEVIIEECPQLKTL 606
           +LP L+ + N     + ++  +L+ + +E C  LK L
Sbjct: 894 YLPQLRSLCN-----DRVVLESLEHLEVESCESLKNL 895

BLAST of Lsi10G006250 vs. ExPASy Swiss-Prot
Match: Q9T048 (Disease resistance protein At4g27190 OS=Arabidopsis thaliana OX=3702 GN=At4g27190 PE=2 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 9.7e-34
Identity = 158/651 (24.27%), Postives = 287/651 (44.09%), Query Frame = 0

Query: 4   YIQEIAKDVVKECGGLPIALKILAKALKGKR-VEIWKDTCKKLKDPILVNIPGVSDQLYS 63
           ++++IAK V +ECGGLP+A+  +  A++GK+ V++W     KL   +   I  + ++++ 
Sbjct: 323 HVRKIAKAVSQECGGLPLAIITVGTAMRGKKNVKLWNHVLSKLSKSV-PWIKSIEEKIFQ 382

Query: 64  CLQFSYDSIEDEAKLILFLCCVFPDDHRINLKDLQMYALSMRLLNGISAWRDARNRVIKL 123
            L+ SYD +ED+AK    LC +FP+D+ I + ++  Y ++   +  + +  D+ N  I  
Sbjct: 383 PLKLSYDFLEDKAKFCFLLCALFPEDYSIEVTEVVRYWMAEGFMEELGSQEDSMNEGITT 442

Query: 124 VDDLKSSSLLLKSDNGNDCVQMHDVVHDVAKYI--ASKIHKMPSLSYGSGESEWRDEDIC 183
           V+ LK   LL   D   D V+MHDVV D A +I  +S+      +  G+G  + R + + 
Sbjct: 443 VESLKDYCLLEDGDR-RDTVKMHDVVRDFAIWIMSSSQDDSHSLVMSGTGLQDIRQDKLA 502

Query: 184 GSYNAIYINCKKFLNFPPKLEFPNLQLFILSGVSDFRSAIRIPDTFFEGMKELRVLDV-- 243
            S   + +   K  + P  +E   ++  +L    +F     +P  F +    LR+L++  
Sbjct: 503 PSLRRVSLMNNKLESLPDLVEEFCVKTSVLLLQGNFLLK-EVPIGFLQAFPTLRILNLSG 562

Query: 244 -RTMCFAPSSWTSLTQSLKNLQTLCLTMREGSDFGTIGELKKLEILKIISSSFFQVLPAS 303
            R   F   S   L          C  + +     ++  L KLE+L +  +   +  P  
Sbjct: 563 TRIKSFPSCSLLRLFSLHSLFLRDCFKLVK---LPSLETLAKLELLDLCGTHILE-FPRG 622

Query: 304 MSELTQLKVLHI-LCPGLKLIHPNILTSMRRLEELIVGCDYKRWGDE-VTYKNKLIKNVK 363
           + EL + + L +     L+ I   +++ +  LE L +   + RW  +  T K +      
Sbjct: 623 LEELKRFRHLDLSRTLHLESIPARVVSRLSSLETLDMTSSHYRWSVQGETQKGQ----AT 682

Query: 364 LSELDCLTRLLFLKLNIQNVDILSNELSLDRAQSLEEFHIQVGN-YVYSLRSDKFKTSLE 423
           + E+ CL RL  L + + +   L N+ +    + L++F + VG+ Y+   R DK + ++ 
Sbjct: 683 VEEIGCLQRLQVLSIRLHSSPFLLNKRN-TWIKRLKKFQLVVGSRYILRTRHDKRRLTIS 742

Query: 424 LETKSPIGSIGGVL----QIILQKCENLVVKDSVDFTKLFCNPNGNYGYPRLKHLQI--- 483
               S + SIG +L     + L  C+ +         KL    + N G+  LK L I   
Sbjct: 743 HLNVSQV-SIGWLLAYTTSLALNHCQGI----EAMMKKLV---SDNKGFKNLKSLTIENV 802

Query: 484 ---------IIREQDEDMPHGIRNITPHFDLQLWHHNNGLTANSFCKLQR---------- 543
                    ++          I ++ P+ + +L  H   +   +F +LQ           
Sbjct: 803 IINTNSWVEMVSTNTSKQSSDILDLLPNLE-EL--HLRRVDLETFSELQTHLGLKLETLK 862

Query: 544 -ICINGCNKLTSVFPSHMVISLKFLNTLEVRYCKLVERIFEVEEQSNGVNQVMPMVSTKL 603
            I I  C KL ++      +++  L  +E+ YC  ++ + E          V  +   KL
Sbjct: 863 IIEITMCRKLRTLLDKRNFLTIPNLEEIEISYCDSLQNLHEALLYHQPF--VPNLRVLKL 922

Query: 604 NFLPNLKHMWNNDSARELLIFPNLKEVIIEECPQLKTLFPASFINHMKKIE 619
             LPNL  + N     E      L++V +  C QL  L  +S    +KKI+
Sbjct: 923 RNLPNLVSICNWGEVWEC-----LEQVEVIHCNQLNCLPISSTCGRIKKIK 943

BLAST of Lsi10G006250 vs. ExPASy Swiss-Prot
Match: Q8RXS5 (Probable disease resistance protein At5g63020 OS=Arabidopsis thaliana OX=3702 GN=At5g63020 PE=2 SV=2)

HSP 1 Score: 95.5 bits (236), Expect = 3.3e-18
Identity = 156/618 (25.24%), Postives = 260/618 (42.07%), Query Frame = 0

Query: 5   IQEIAKDVVKECGGLPIALKILAKALKGKR-VEIWKDTCKKLKDPILVNIPGVSDQLYSC 64
           I  +A+ V K+C GLP+AL ++ + +  KR V+ W+     L         G+ D++   
Sbjct: 335 IPTVARTVAKKCRGLPLALNVIGETMAYKRTVQEWRSAIDVLTSS-AAEFSGMEDEILPI 394

Query: 65  LQFSYDSIEDE-AKLILFLCCVFPDDHRINLKDLQMYALSMRLLNGISAWRDARNRVIKL 124
           L++SYD+++ E  KL    C +FP+DH I   DL  Y +    ++       A N+  ++
Sbjct: 395 LKYSYDNLKSEQLKLCFQYCALFPEDHNIEKNDLVDYWIGEGFIDRNKG--KAENQGYEI 454

Query: 125 VDDLKSSSLLLKSDNGNDCVQMHDVVHDVAKYIASKIHKMPS--------LSYGSGESE- 184
           +  L  S LL+  +   + V+MHDVV ++A +IAS   K            S    E E 
Sbjct: 455 IGILVRSCLLM--EENQETVKMHDVVREMALWIASDFGKQKENFIVQAGLQSRNIPEIEK 514

Query: 185 WRDEDICGSYNAIYINCKKFLNFPPKLEFPNLQLFILSGVSDFRSAIRIPDTFFEGMKEL 244
           W+   +    + ++ N +   + P   E P L   +L    +F     I  +FF  M  L
Sbjct: 515 WK---VARRVSLMFNNIESIRDAP---ESPQLITLLLR--KNFLG--HISSSFFRLMPML 574

Query: 245 RVLDV---RTMCFAPSSWTSLTQSLKNLQTLCLTMREGSDF-GTIGELKKLEILKIISSS 304
            VLD+   R +   P+  +       +LQ L L+      +   + EL+KL  L +  + 
Sbjct: 575 VVLDLSMNRDLRHLPNEISECV----SLQYLSLSRTRIRIWPAGLVELRKLLYLNLEYTR 634

Query: 305 FFQVLPASMSELTQLKVLHILCPGLKLIHPNILTSMRRLEEL-----IVGCDYKRWGDEV 364
             + +   +S LT LKVL +   G     P +L  ++ LE L      +G          
Sbjct: 635 MVESI-CGISGLTSLKVLRLFVSGFP-EDPCVLNELQLLENLQTLTITLGL--------A 694

Query: 365 TYKNKLIKNVKLSELDCLTRLLFLKLNIQNVDILSNELSLDRAQSLEEFHIQVGNYVYSL 424
           +   + + N +L+   C   L    LN Q+  ++S   ++D   SL+E H    + ++ +
Sbjct: 695 SILEQFLSNQRLA--SCTRALRIENLNPQS-SVISFVATMD---SLQELHF-ADSDIWEI 754

Query: 425 RSDKFKTSLELETKSPIGSIGGVLQIILQKCENLVVKDSVDFTKLFCNPNGNYGYPRLKH 484
           +  + +T L L   +       + Q+ L+ C  L      D T L   PN       L  
Sbjct: 755 KVKRNETVLPLHIPTTTTFFPNLSQVSLEFCTRL-----RDLTWLIFAPN-------LTV 814

Query: 485 LQII----IREQDEDMPHGIRNITPHFDLQ---------LWHHNNGLTANSFCKLQRICI 544
           L++I    ++E         +N+ P  +L+         L H + G     F  LQ+I +
Sbjct: 815 LRVISASDLKEVINKEKAEQQNLIPFQELKELRLENVQMLKHIHRG--PLPFPCLQKILV 874

Query: 545 NGCNKL-------TSVFPSHMVISLKFLNTLEVRYCKLVERIFEVEEQSNGVNQVMPMVS 583
           NGC++L       TSV    +VI           + K +E I E E++           +
Sbjct: 875 NGCSELRKLPLNFTSVPRGDLVIE---------AHKKWIE-ILEWEDE-----------A 881

BLAST of Lsi10G006250 vs. ExPASy Swiss-Prot
Match: Q9C8T9 (Putative disease resistance protein At1g63350 OS=Arabidopsis thaliana OX=3702 GN=At1g63350 PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.1e-16
Identity = 84/336 (25.00%), Postives = 157/336 (46.73%), Query Frame = 0

Query: 5   IQEIAKDVVKECGGLPIALKILAKALKGKR-VEIWKDTCKKLKDPILVNIPGVSDQLYSC 64
           I+E+++ V K+C GLP+AL ++++ +  KR V+ W+     L +       G+ D++   
Sbjct: 333 IRELSRVVAKKCCGLPLALNVVSETMSCKRTVQEWRHAIYVL-NSYAAKFSGMDDKILPL 392

Query: 65  LQFSYDSIE-DEAKLILFLCCVFPDDHRINLKDLQMYALSMRLLNGISAWRDARNRVIKL 124
           L++SYDS++ ++ K+ L  C +FP+D +I  ++L  Y +   +++G      A N+  ++
Sbjct: 393 LKYSYDSLKGEDVKMCLLYCALFPEDAKIRKENLIEYWICEEIIDGSEGIDKAENQGYEI 452

Query: 125 VDDLKSSSLLLKSD--NGNDCVQMHDVVHDVAKYIASKIHKMPSLSYGSGESEWRDEDIC 184
           +  L  +SLL++    +G + V +HDVV ++A +IAS + K             R+    
Sbjct: 453 IGSLVRASLLMEEVELDGANIVCLHDVVREMALWIASDLGKQNEAFIVRASVGLREILKV 512

Query: 185 GSYNAI---YINCKKFLNFPPKLEFPNLQLFILSGVSDFRSAIRIPDTFFEGMKELRVLD 244
            ++N +    +      +   +L+   L   +L          +I   FF  M +L VLD
Sbjct: 513 ENWNVVRRMSLMKNNIAHLDGRLDCMELTTLLLQST----HLEKISSEFFNSMPKLAVLD 572

Query: 245 VR----------------TMCFAPSSWTSLTQSLKNLQTL----CLTMREGSDFGT---I 304
           +                 ++ +   S T +    K LQ L     L +   S  G+   I
Sbjct: 573 LSGNYYLSELPNGISELVSLQYLNLSSTGIRHLPKGLQELKKLIHLYLERTSQLGSMVGI 632

Query: 305 GELKKLEILKIISSSFFQVLPASMSELTQLKVLHIL 311
             L  L++LK+  SS+   L  ++ EL  L+ L +L
Sbjct: 633 SCLHNLKVLKLSGSSYAWDLD-TVKELEALEHLEVL 662

BLAST of Lsi10G006250 vs. ExPASy Swiss-Prot
Match: Q9FG91 (Probable disease resistance protein At5g43730 OS=Arabidopsis thaliana OX=3702 GN=At5g43730 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 2.9e-14
Identity = 93/345 (26.96%), Postives = 157/345 (45.51%), Query Frame = 0

Query: 5   IQEIAKDVVKECGGLPIALKILAKALKGKR-VEIWKDTCKKLKDPILVNIPGVSDQLYSC 64
           I  +A+ V  +C GLP+AL ++ KA+  K  V+ W+     L  P     PG+ +++   
Sbjct: 332 IPALARIVAAKCHGLPLALNVIGKAMVCKETVQEWRHAINVLNSP-GHKFPGMEERILPI 391

Query: 65  LQFSYDSIED-EAKLILFLCCVFPDDHRINLKDLQMYALSMRLLNGISAWRDARNRVIKL 124
           L+FSYDS+++ E KL    C +FP+D  I    L  Y +    +N         N+   +
Sbjct: 392 LKFSYDSLKNGEIKLCFLYCSLFPEDFEIEKDKLIEYWICEGYINPNRYEDGGTNQGYDI 451

Query: 125 VDDLKSSSLLLKSDNGNDCVQMHDVVHDVAKYIASKI-HKMPSLSYGSG--------ESE 184
           +  L  + LL++ +   D V+MHDV+ ++A +I S   ++  ++   SG        +  
Sbjct: 452 IGLLVRAHLLIECEL-TDKVKMHDVIREMALWINSDFGNQQETICVKSGAHVRLIPNDIS 511

Query: 185 WRDEDICGSYNAIYINCKKFLNFPPKLEFPNLQLFILSGVSDFRSAIRIPDTFFEGMKEL 244
           W   +I    + I    +K    P     PNL   +L     +   + I   FF  M +L
Sbjct: 512 W---EIVRQMSLISTQVEKIACSP---NCPNLSTLLL----PYNKLVDISVGFFLFMPKL 571

Query: 245 RVLDVRTMCFAPSSWTSL-----TQSLKNLQTLCLTMREGSDFGTIGELKKLEILKIISS 304
            VLD+ T      +W+ +       +L +LQ L L++  G     +G LKKL  L  ++ 
Sbjct: 572 VVLDLST------NWSLIELPEEISNLGSLQYLNLSL-TGIKSLPVG-LKKLRKLIYLNL 631

Query: 305 SFFQVLPASMSELTQLKVLHI--LCPGLKLIHPNILTSMRRLEEL 332
            F  VL + +   T L  L +  L   L  +   I+  ++RL+ L
Sbjct: 632 EFTNVLESLVGIATTLPNLQVLKLFYSLFCVDDIIMEELQRLKHL 656

BLAST of Lsi10G006250 vs. ExPASy TrEMBL
Match: A0A6J1CPP4 (probable disease resistance protein At4g27220 OS=Momordica charantia OX=3673 GN=LOC111013433 PE=4 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 7.0e-221
Identity = 450/889 (50.62%), Postives = 575/889 (64.68%), Query Frame = 0

Query: 5    IQEIAKDVVKECGGLPIALKILAKALKGKRVEIWKDTCKKLKDPILVNIPGVSDQLYSCL 64
            I+ IAK+VVK+CGGLPIALKI+AKALKGK + IWKD  +KL+  +LV I GVSDQLYSCL
Sbjct: 335  IEHIAKNVVKKCGGLPIALKIIAKALKGKEIHIWKDAFEKLEKHVLVKIKGVSDQLYSCL 394

Query: 65   QFSYDSIED-EAKLILFLCCVFPDDHRINLKDLQMYALSMRLLNGISAWRDARNRVIKLV 124
            + SYD IED E +L+  LC VFPDDH I +KDLQMYA+ M LLN I+ W   +NRVI LV
Sbjct: 395  KLSYDWIEDEETRLLFLLCSVFPDDHNICVKDLQMYAMGMGLLNDINNWEATKNRVIDLV 454

Query: 125  DDLKSSSLLLKSDNGNDCVQMHDVVHDVAKYIASKIHKMPSLSYGSGESEWRDEDICGSY 184
            DDLKSS LLL+S++G++ VQMHDVV DVAKYIAS   KM SLSYG G+SEW +ED  GSY
Sbjct: 455  DDLKSSYLLLESNSGDNYVQMHDVVRDVAKYIASNDDKMSSLSYGCGQSEWLEEDRSGSY 514

Query: 185  NAIYINCKKFLNFPPKLEFPNLQLFILSGVSDF--RSAIRIPDTFFEGMKELRVLDVRTM 244
            NAI+++C  F + P   EFPNLQL IL   S F    AI+IP  FFEGM++LRVLD+  M
Sbjct: 515  NAIFVDCMDFSSLPSNFEFPNLQLLILGMPSFFGEGEAIQIPGAFFEGMEKLRVLDMTKM 574

Query: 245  CFAPSSWTSLTQSLKNLQTLCLTMREGSDFGTIGELKKLEILKIISSSFFQVLPASMSEL 304
            CF PS  TS+T SLKNLQTLC++  E  D  TIG+LKKL+ILK I+   F+ LPA+MS+L
Sbjct: 575  CFEPSR-TSVTHSLKNLQTLCMSYCEYYDIDTIGQLKKLKILK-INDCTFEELPANMSQL 634

Query: 305  TQLKVLHIL-CPGLKLIHPNILTSMRRLEELIVGCDYKRWGDEVTYKNKLIKNVKLSELD 364
            TQL++L +  C  LK+I  NI++SM +LEEL +   +KRWG+E++Y+NKLI+NVKLSEL+
Sbjct: 635  TQLRLLDLSNCSNLKVIPINIISSMTKLEELNLWRSFKRWGEEISYRNKLIQNVKLSELN 694

Query: 365  CLTRLLFLKLNIQNVDILSNELSLDRAQSLEEFHIQVGNYVYSLR-SDKFKTSLELETKS 424
             L+RL  L+L I NV+I S++LSL+R + LEEF I VGN  +  R  D +   L +  KS
Sbjct: 695  HLSRLSNLRLEIPNVNIFSDDLSLERVEKLEEFCICVGNDSWGFRICDGYARRLMVNIKS 754

Query: 425  PIGSIGGVLQIILQKCENLVVKDSVDFTKLFCNPNGNYGYPRLKHLQIIIREQDEDMPHG 484
             I +IGG LQI+L+ CE L ++D+V FT      NGNY  PRLKHL I   E + +MP  
Sbjct: 755  QIVAIGGTLQILLEGCEQLYIEDTVGFTNCLFKLNGNYCNPRLKHLHI---EGNSEMPRR 814

Query: 485  I-----------RNITPHFDLQLWHHNNGLTANSFCKLQRICINGCNKLTSVFPSHMVIS 544
            I             I    +L++  HNN L ANSFCKL+ ICI+ CNKLT +F S+MV S
Sbjct: 815  IDVQVSFPELKELTIQGGNNLEMLWHNNRLIANSFCKLRSICISCCNKLTYMFTSNMVTS 874

Query: 545  LKFLNTLEVRYCKLVERIFEVEEQSNGVNQVMPMVSTKLNFLPNLKHMWNNDSARELLIF 604
            L FLNTLE+  C+L+ERIFE+ +  +   +V+P+    L  LPNLK++WNND   E L F
Sbjct: 875  LVFLNTLEIDNCELLERIFEIGKLVDDA-KVLPLTDLSLRSLPNLKYVWNNDPG-EFLTF 934

Query: 605  PNLKEVIIEECPQLKTLFPASFINHMKKIEKLYVDGENQIFAEDEASHL-------RKLT 664
            PNLK V +  CP+LK LFPASFI H+++IE L     ++IF+EDEAS L       R L 
Sbjct: 935  PNLKNVNVSSCPRLKCLFPASFIKHIEEIESLCAYEVDEIFSEDEASKLGFPEIVFRSLQ 994

Query: 665  MHRKLVKEGSFWVISELFPKLHTFSLIGSKDDGTVSLPSEMSELVYRVKAIEIRNALELI 724
            +   +VK  SFW  S  FPKLH   L G +DD  V+LP EMS+ +Y              
Sbjct: 995  ILEMVVKR-SFWFRSGSFPKLHNLKLKGGEDDDLVTLPLEMSQELY-------------- 1054

Query: 725  LMFQNGKENVKNSHQIFTSIGLDKLPNFTHVWEDNNDQMMSRLKNIYVQGYDDLKCLVPS 784
                                                        NI V+  D LK L+PS
Sbjct: 1055 --------------------------------------------NIEVKKCDKLKFLLPS 1114

Query: 785  SITFLNLTSFMVINCKGMINLFSSSMVKNLVNLKVMDITGCEGMISIVIAEEGGRQREDI 844
            S TF NL S ++ +C GM+NLF++ + KNLVNL ++ I  C G+ SIV  E    + E+I
Sbjct: 1115 STTFFNLGSLIIEDCNGMMNLFNTLVAKNLVNLCLIKICDCRGITSIVAEEVEEEEGEEI 1157

Query: 845  IFNSLHILKLYKISRLTDFYSGKYMLKFPCSEKLSIKQCSEMKTFSYGI 871
            IFN L  L+L  + RL +FYSGK  LKFP  E+L I+ C +MKTFS+GI
Sbjct: 1175 IFNHLIRLELRDLPRLANFYSGKCALKFPYLERLIIEGCPKMKTFSFGI 1157

BLAST of Lsi10G006250 vs. ExPASy TrEMBL
Match: A0A1S3CHN4 (disease resistance protein At4g27190-like OS=Cucumis melo OX=3656 GN=LOC103501064 PE=4 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 2.3e-147
Identity = 363/995 (36.48%), Postives = 538/995 (54.07%), Query Frame = 0

Query: 4    YIQEIAKDVVKECGGLPIALKILAKALKGKRVEIWKDTCKKLKDPILVNIPGVSDQLYSC 63
            Y + IAK+V KECGGLP+AL  +AKALKGK +  W+D   KL+D I ++I GVSD++Y+ 
Sbjct: 317  YKKNIAKNVAKECGGLPLALDTIAKALKGKDMHHWEDALTKLRDSIGMDIKGVSDKVYAS 376

Query: 64   LQFSYDSIE-DEAKLILFLCCVFPDDHRINLKDLQMYALSMRLLNGISAWRDARNRVIKL 123
            L+ SY+ ++ +E KL+  LC VF DD++I +KDLQMYA+ MRLLN +  W D++NRV+KL
Sbjct: 377  LRLSYEHLDGEETKLLFLLCSVFADDYKIPIKDLQMYAMGMRLLNKVKTWEDSKNRVMKL 436

Query: 124  VDDLKSSSLLL--KSDNGNDCVQMHDVVHDVAKYIASKIHKMPSLSYGSGE-SEWRDEDI 183
            V+DL SSSLLL  +SD+ +  V+MH+VV DVA +IASK   M + + G  + +EW DE  
Sbjct: 437  VNDLISSSLLLEAESDSKDKYVKMHNVVRDVAIHIASKEGNMSTFNIGYNKVNEWEDEYR 496

Query: 184  CGSYNAIYINCKKFLNFPPKLEFPNLQLFILSGVSDF--RSAIRIPDTFFEGMKELRVLD 243
             GS+ AI+ NC  F N P K+ FP L+L IL  VS++   + ++IP  FF+GM++L+VLD
Sbjct: 497  SGSHRAIFANCDNFNNLPLKMNFPQLELLILR-VSNWLVENNLQIPYAFFDGMEKLKVLD 556

Query: 244  VRTMCFAPSSWTSLTQSLKNLQTLCLTMREGSDFGTIGELKKLEILKIISSSFFQVLPAS 303
            +  MC     WT  T SL NL+TLC+   E +D  TIGELKKLE+L+I+  +    LP +
Sbjct: 557  LTGMCCLRPLWT--TPSLNNLRTLCMLRCEFNDIDTIGELKKLEVLRIVKCNMLDHLPPT 616

Query: 304  MSELTQLKVLHIL-CPGLKLIHPNILTSMRRLEELIVGCDYKRWGDEVTYKNKLIKNVKL 363
            MS+LTQLKVL +L C  L+++  N+ +SM +LEEL +   + RWG+EV YK++L+KNV +
Sbjct: 617  MSQLTQLKVLEVLNCSKLEVVPANVFSSMTKLEELKLQDSFCRWGEEVWYKDRLVKNVTV 676

Query: 364  SELDCLTRLLFLKLNIQNVDILSNELSLDRAQSLEEFHI---QVGNYVYSLRSDKFKTSL 423
            SEL+CL  L  L L   NV ILS E++    + L+EF I   +  +++    S+++ T+L
Sbjct: 677  SELNCLPCLSNLSLESWNVKILS-EINSQTCKKLKEFWICSNESDDFLQPKASNEYATTL 736

Query: 424  ELETKSPIGSIGGVLQIILQKCENLVVKDSV-DFTKLFCNPNGNYGYPRLKHLQIIIREQ 483
             L  +S IGSI   L+I+LQ+ E L+V DS  +F      PNG  GYP LK+L II    
Sbjct: 737  MLNIESQIGSIDEGLEILLQRSERLIVSDSKGNFINAIFKPNGLNGYPYLKYLWIIDENG 796

Query: 484  DEDMPHGIRNITPHFDLQLWHHNNGLTANSFCKLQRICINGCNKLTSVFPSHMVIS-LKF 543
            + +MPH                   L  + F  L+ + I G  +L ++ P H+ IS  K 
Sbjct: 797  NSEMPH-------------------LIGSDFTSLKYLIIFGMKRLENIVPRHISISPFKS 856

Query: 544  LNTLEVRYCKLVERIF----------------------------EVEEQSN--------- 603
            + T+ +++C  +  +F                            E+E+Q N         
Sbjct: 857  VKTIAIQFCGQIRNLFSFSIFKDLLDLQEIEVINCGNMEGIVFMEIEDQLNICTSPLTSL 916

Query: 604  -------------------GVNQVMPMVSTKLNFLP----------NLKHMWNNDSAREL 663
                                   ++P    +++F            NL+ +W+ ++    
Sbjct: 917  QLENVDKLTSFCTKGLVQESSQSIIPFFDGQVSFPELNDLSIVGGYNLETLWHKNNNPTT 976

Query: 664  LIFPNLKEVIIEECPQLKTLFPASF---INHMKKIEKLYVDGENQIFAEDEASHLRKLTM 723
              F  L+ + IE+C QL+ +FP++    +  +  I+ +      +IF  +  S   K  +
Sbjct: 977  NSFCKLQSIRIEQCTQLRCMFPSNMFASLTSLHTIQIISCASLKRIFEIENQSFSVKTVV 1036

Query: 724  HRKLVKEGSFWVISELFPKLHTFSLIGSKDDGTVSLPSEMSELVYRVKAIEIRNALELIL 783
                      W ++E    LH   L   K              V+R   I+       IL
Sbjct: 1037 ----------WSLNE----LHLLDLPNLKH-------------VWRKDIIK-------IL 1096

Query: 784  MFQNGKENVKNSHQIFTSIGLDKLPNFTHVWEDNNDQMMS--RLKNIYVQGYDDLKCLVP 843
             F + K            + +      THVW+DN  +  S   L+ I V+   +LK L+P
Sbjct: 1097 TFPSLKR-----------VKIHGCTKLTHVWKDNTKETRSFDSLERIEVEKCKNLKYLLP 1156

Query: 844  SSITFLNLTSFMVINCKGMINLFSSSMVKNLVNLKVMDITGCEGMISIVIAEEGGRQRED 903
            SSITFLNL    +  C GM+NLFSS++ K LVNL+ + ++ C+ M  +V   EG ++  D
Sbjct: 1157 SSITFLNLKELHIKKCNGMMNLFSSTVTKKLVNLRSIKVSYCKRMRCMV---EGDQEEND 1216

Query: 904  --IIFNSLHILKLYKISRLTDFYSGKYMLKFPCSEKLSIKQCSEMKTFSYGIINTPKLKH 913
              I+F  L  L+L  + RL  F+SGK ML+FPC E L IK+C EMKTFSYG+I  P+L  
Sbjct: 1217 EIIVFKRLSRLELDYLPRLDSFHSGKCMLEFPCLESLVIKRCPEMKTFSYGVIIAPRLHS 1236

BLAST of Lsi10G006250 vs. ExPASy TrEMBL
Match: A0A0A0K5R3 (NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G239020 PE=4 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 5.7e-146
Identity = 387/1086 (35.64%), Postives = 555/1086 (51.10%), Query Frame = 0

Query: 5    IQEIAKDVVKECGGLPIALKILAKALKGKR--VEIWKDTCKKLKDPILVNIPGVSDQLYS 64
            ++ IAK+V K+CGGLP+AL I+AK LK  R     W+    KLK+ I VNI  V +++Y+
Sbjct: 325  MENIAKEVAKQCGGLPLALDIIAKTLKRSRHINYYWEGVLSKLKNSIPVNI-DVGEKVYA 384

Query: 65   CLQFSYDSIE-DEAKLILFLCCVFPDDHRINLKDLQMYALSMRLLNGISAWRDARNRVIK 124
             L+ SY+ ++ +E K +  LC VFPDDH I++ DLQMY + M LL  ++ W++AR     
Sbjct: 385  SLKLSYEHLDGEEVKSLFLLCSVFPDDHGISVNDLQMYVMGMGLLKMVNTWKEARAEAHY 444

Query: 125  LVDDLKSSSLLLKSDNGNDCVQMHDVVHDVAKYIASKIHKMPSLSYG-SGESEWRDEDIC 184
            LV+DL SSSLL +  N +  V+MHD+V DVA YI    + M +L YG S  S+  DED C
Sbjct: 445  LVEDLTSSSLLQRLKNRD--VKMHDIVRDVAIYIGPDFN-MSTLYYGYSTSSKGLDEDKC 504

Query: 185  GSYNAIYINCKKFLNFPPKLEFPNLQLFILSGVSDFRSA-IRIPDTFFEGMKELRVLDVR 244
             SY AI+++CKKF N  P L+ P L+L ILS     +   I I D +FEGM+ L+VLD+ 
Sbjct: 505  RSYRAIFVDCKKFCNLLPNLKLPKLELLILSFPFWGKDRNIDIMDAYFEGMENLKVLDIE 564

Query: 245  TMCFAPSSWTSLTQSLKNLQTLCLTMREGSDFGTIGELKKLEILKIISSSFFQVLPASMS 304
               F    WT     LKNL+TLC++     D  TIG LK+LEIL+I +      LP SMS
Sbjct: 565  GTSFLQPFWT----PLKNLRTLCMSYCWCEDIDTIGHLKQLEILRISNCRGITELPTSMS 624

Query: 305  ELTQLKVLHIL-CPGLKLIHPNILTSMRRLEELIVGCDYKRWGDEVTYKNKLIKNVKLSE 364
            EL QLKVL +  C  L +IH NI++SM +LEEL +   +K WG+EV YKN  I N +LSE
Sbjct: 625  ELKQLKVLVVSHCFKLVVIHTNIISSMTKLEELDIQDCFKEWGEEVRYKNTWIPNAQLSE 684

Query: 365  LDCLTRLLFLKLNIQNVDILSNELSLDRAQSLEEFHIQVGNY---VYSLRS----DKFKT 424
            L+CL+ L  L++ +  + ILS  LS    ++L EF I VG +    +  +S    DK++ 
Sbjct: 685  LNCLSHLSILRVRVLKLTILSEALSSQMLKNLREFFIYVGTHEPKFHPFKSWSSFDKYEK 744

Query: 425  SLELETKSPIGSIGGV-LQIILQKCENL-VVKDSVDFTKLFCNPNGNYGYPRLKHLQIII 484
            ++    KS I S+ G  L I+L+  + L ++ DS  F        GN GYP LK L+I  
Sbjct: 745  NMSFNMKSQIVSVNGTKLSILLEGTKRLMILNDSKGFANDIFKAIGN-GYPLLKCLEI-- 804

Query: 485  REQDEDMPH--------------------------------------------------- 544
               + + PH                                                   
Sbjct: 805  -HDNSETPHLRGNDFTSLKRLVLDRMVMLESIIPRHSPINPFNKLKFIKIGRCEQLRNFF 864

Query: 545  ------GIRN-------------------------------------------------- 604
                  G+ N                                                  
Sbjct: 865  PLSVFKGLSNLRQIEIYECNMMEEIVSIEIEDHITIYTSPLTSLRIERVNKLTSFCSTKS 924

Query: 605  -----ITPHFDLQ--------------------LWHHNNGLTANSFCKLQRICINGCNKL 664
                 I P FD +                    LWH N     +SF KLQ I I+ C +L
Sbjct: 925  SIQQTIVPLFDERRVSFPELKYLSIGRANNLEMLWHKN----GSSFSKLQTIEISDCKEL 984

Query: 665  TSVFPSHMVISLKFLNTLEVRYCKLVERIFEVEEQ-SNGVNQVMPMVSTKLNFLPNLKHM 724
              VFPS++  SL FL+TL++  C+L+E IFE+E+Q ++G  +V+P+    L FL NLK++
Sbjct: 985  RCVFPSNIATSLVFLDTLKIYGCELLEMIFEIEKQKTSGDTKVVPLRYLSLGFLKNLKYV 1044

Query: 725  WNNDSARELLIFPNLKEVIIEECPQLKTLFPASFINHMKKIEKL-YVDGEN-QIFAEDEA 784
            W+ D   +++ FPNLK+V +  CP+LK +FPASF  +MK+IE+L  V+  N +IF  DEA
Sbjct: 1045 WDKD-VDDVVAFPNLKKVKVGRCPKLKIIFPASFTKYMKEIEELEMVEPFNYEIFPVDEA 1104

Query: 785  SHLRKLTMHRKL----------VKEGSFWVISELFPKLHTFSLIGSKDDGTVSLPSEMSE 844
            S L+++ + + L          VKE  FWV+S+ F KL +  L G +D   +SLP EM+E
Sbjct: 1105 SKLKEVALFQSLETLRMSCKQAVKE-RFWVMSKFF-KLKSLELFGCEDGKMISLPMEMNE 1164

Query: 845  LVYRVKAIEIRNALELILMFQNGKENVKNSHQIFTSIGLDKLPNFTHVWEDNNDQMMSRL 904
            ++Y ++ + IR  L+L+       + + N + I     L KL  +      N  ++M  L
Sbjct: 1165 VLYSIEELTIRGCLQLV-------DVIGNDYYIQRCANLKKLKLY------NLPKLMYVL 1224

Query: 905  KNIYVQGYDDLKCLVPSSITFLNLTSFMVINCKGMINLFSSSMVKNLVNLKVMDITGCEG 922
            KN+             ++ TF  L    V  C GMINLFS S+ KNL NL  ++I  C  
Sbjct: 1225 KNMNQM----------TATTFSKLVYLQVGGCNGMINLFSPSVAKNLANLNSIEIYDCGE 1284

BLAST of Lsi10G006250 vs. ExPASy TrEMBL
Match: A0A0A0LTN4 (NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G433370 PE=4 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 9.7e-146
Identity = 352/923 (38.14%), Postives = 526/923 (56.99%), Query Frame = 0

Query: 4    YIQEIAKDVVKECGGLPIALKILAKALKGKRVEIWKDTCKKLKDPILVNIPGVSDQLYSC 63
            Y + IAK+V KECGGLP+AL  +AKALKGK +  W+D   KL++ I ++I GVSD++Y+ 
Sbjct: 317  YKKNIAKNVAKECGGLPLALDTIAKALKGKDMHHWEDALTKLRNSIGMDIKGVSDKVYAS 376

Query: 64   LQFSYDSIE-DEAKLILFLCCVFPDDHRINLKDLQMYALSMRLLNGISAWRDARNRVIKL 123
            L+ SYD ++ +E KLI  LC VFPDD++I++K+LQMYA+ MRLLN +  W D++NRV+KL
Sbjct: 377  LRLSYDHLDGEETKLIFLLCSVFPDDYKISIKNLQMYAMCMRLLNKVKTWEDSKNRVMKL 436

Query: 124  VDDLKSSSLLL--KSDNGNDCVQMHDVVHDVAKYIASKIHKMPSLSYGSGE-SEWRDEDI 183
            V+DL SSSLLL  +SD+ +  V+MHDVV DVA +IASK   M +L+ G  + +EW DE  
Sbjct: 437  VNDLISSSLLLEAESDSKDKYVKMHDVVRDVAIHIASKEGNMSTLNIGYNKVNEWEDECR 496

Query: 184  CGSYNAIYINCKKFLNFPPKLEFPNLQLFILSGVSDF--RSAIRIPDTFFEGMKELRVLD 243
             GS+ AI+ NC    N P K+ FP L+L IL  VS +     ++IP  FF+GM +L+VLD
Sbjct: 497  SGSHRAIFANCDNLNNLPLKMNFPQLELLILR-VSYWLVEDNLQIPYAFFDGMVKLKVLD 556

Query: 244  VRTMCFAPSSWTSLTQSLKNLQTLCLTMREGSDFGTIGELKKLEILKIISSSFFQVLPAS 303
            +  MC     WT  T SL NLQ LC+   E +D  TIGELKKLE+L+I+  +    LP +
Sbjct: 557  LTGMCCLRPLWT--TPSLNNLQALCMLRCEFNDIDTIGELKKLEVLRIVKCNMLDHLPPT 616

Query: 304  MSELTQLKVLHIL-CPGLKLIHPNILTSMRRLEELIVGCDYKRWGDEVTYKNKLIKNVKL 363
            MS+LT LKVL +L CP L+++  NI +SM +LEEL +   + RWG+EV YK++L+KNV +
Sbjct: 617  MSQLTHLKVLEVLNCPKLEVVPANIFSSMTKLEELKLQDSFCRWGEEVWYKDRLVKNVTV 676

Query: 364  SELDCLTRLLFLKLNIQNVDILSNELSLDRAQSLEEFHI---QVGNYVYSLRSDKFKTSL 423
            SEL+CL  L  L L   NV ILS E+S    + L+EF I   +  +++    S+++ T+L
Sbjct: 677  SELNCLPCLSNLSLESWNVKILS-EISSQTCKKLKEFWICSNESDDFIQPKVSNEYATTL 736

Query: 424  ELETKSPIGSIGGVLQIILQKCENLVVKDSV-DFTKLFCNPNGNYGYPRLKHLQIIIREQ 483
             L  +S +GSI   L+I+LQ+ E L+V DS  +F      PNGN GYP LK+L +I    
Sbjct: 737  MLNIESQVGSIDEGLEILLQRSERLIVSDSKGNFINAMFKPNGN-GYPCLKYLWMIDENG 796

Query: 484  DEDMPHGIRNITPHFDLQLWHHNNGLTANSFCKLQRICINGCNKLTSVFPSHMVIS-LKF 543
            + +M H                   L  + F  L+ + I G  +L ++ P H+ +S  K 
Sbjct: 797  NSEMAH-------------------LIGSDFTSLKYLIIFGMKRLENIVPRHISLSPFKK 856

Query: 544  LNTLEVRYCKLVERIFEVEEQSNGVNQVMPMVSTKLNFLPNLKHMWNNDSARELLIFPNL 603
            + T+ +++C  +  +F               +   L  L  ++ +  N    E +IF  +
Sbjct: 857  VKTIAIQFCGQIRNLFSFS------------IFKDLLDLQEIEVI--NCGKMEGIIFMEI 916

Query: 604  KEVI-IEECPQLKTLFPASFINHMKKIEKLYVDGENQIFAEDEASHLRKLTMHRKLVKEG 663
             + + I  CP L +L        ++ ++KL       +  E   S +             
Sbjct: 917  GDQLNICSCP-LTSL-------QLENVDKLTSFCTKDLIQESSQSII------------- 976

Query: 664  SFWVISELFPKLHTFSLIGSKDDGTV-SLPSEMSELVYRVKAIEIRNALELILMFQNGKE 723
             F+     FP+L+  S++G  +  T+    +  + +++ +  + + N   L  +++    
Sbjct: 977  PFFDGQVSFPELNDLSIVGGNNLETLWHKNNNPTTVLWSLNELHLLNLPNLKQVWR---- 1036

Query: 724  NVKNSHQIFTSIGLDKL-----PNFTHVWEDNN--DQMMSRLKNIYVQGYDDLKCLVPSS 783
              K+  +I T   L ++        THVW+DNN   +    L+ I V+   +LK L+PSS
Sbjct: 1037 --KDIIKILTFPSLKRVKIHGCTKLTHVWKDNNKVTRSFDSLERIEVEKCKNLKYLLPSS 1096

Query: 784  ITFLNLTSFMVINCKGMINLFSSSMVKNLVNLKVMDITGCEGMISIVIAEEGGRQREDII 843
            I FLNL    +  C GMINLFSS++ K LVNL  + ++ C+GM  +V  ++     E I 
Sbjct: 1097 IAFLNLKELHIKKCNGMINLFSSTVTKKLVNLSSIKVSYCKGMRCMVEVDQ-AENDEIIT 1156

Query: 844  FNSLHILKLYKISRLTDFYSGKYMLKFPCSEKLSIKQCSEMKTFSYGIINTPKLKHVEIQ 903
            F  L  L+L  + RL  FYSGK ML+FPC E L IK+C EMKTFSYG+I  P+L+ + + 
Sbjct: 1157 FKKLSTLELDYLPRLDSFYSGKCMLEFPCLESLVIKRCPEMKTFSYGVIIAPRLQTLWMN 1169

Query: 904  EGTQARGTVLVSQARGINVIIED 906
            +    +   + S A GIN  I++
Sbjct: 1217 D----KEFGVSSPACGINETIQN 1169

BLAST of Lsi10G006250 vs. ExPASy TrEMBL
Match: A0A6J1DLB8 (disease resistance protein RPS5-like OS=Momordica charantia OX=3673 GN=LOC111022189 PE=4 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 1.7e-134
Identity = 333/878 (37.93%), Postives = 430/878 (48.97%), Query Frame = 0

Query: 5   IQEIAKDVVKECGGLPIALKILAKALKGKRVEIWKDTCKKLKDPILVNIPGVSDQLYSCL 64
           I+ +A++VVK+CGGLPIALKI+AKALKGK V IWKD  +KL+  + V I GVSDQLYSCL
Sbjct: 335 IEHLARNVVKKCGGLPIALKIIAKALKGKDVRIWKDALEKLEKRVSVKIKGVSDQLYSCL 394

Query: 65  QFSYDSIED-EAKLILFLCCVFPDDHRINLKDLQMYALSMRLLNGISAWRDARNRVIKLV 124
           + SYD IED E +L+  LC VFPDDH+I++KDLQMYA+ M LLN I  W  +RN V++LV
Sbjct: 395 KLSYDRIEDEETRLLFLLCSVFPDDHKIHVKDLQMYAMGMGLLNDIDNWETSRNMVVELV 454

Query: 125 DDLKSSSLLLKSDNGNDCVQMHDVVHDVAKYIASKIHKMPSLSYGSGESEWRDEDICGSY 184
           D LKSSSLLL+S++G++ VQMHDVV DVAKYIA+K +KM SLSYG G+SEWR+++ CGSY
Sbjct: 455 DHLKSSSLLLESNSGDNYVQMHDVVRDVAKYIATKENKMSSLSYGRGQSEWREKERCGSY 514

Query: 185 NAIYINCKKFLNFPPKLEFPNLQLFILSGVSDFRS--AIRIPDTFFEGMKELRVLDVRTM 244
           N I+I+C  F N PP +EFP+LQ+ IL   S F    AI+IP+  FEGMK+LRVLD+  M
Sbjct: 515 NVIFIDCVDFPNLPPNMEFPDLQMLILRIQSFFGEGRAIQIPEALFEGMKKLRVLDMTKM 574

Query: 245 CFAPSSWTSLTQSLKNLQTLCLTMREGSDFGTIGELKKLEILKIISSSFFQVLPASMSEL 304
           CF PS  TS T SLKNLQTL ++  E +D  TI +LKKLEIL+I                
Sbjct: 575 CFEPSK-TSFTHSLKNLQTLRMSYCECNDILTIFQLKKLEILRI---------------- 634

Query: 305 TQLKVLHILCPGLKLIHPNILTSMRRLEELIVGCDYKRWGDEVTYKNKLIKNVKLSELDC 364
                      G+ +I   +  S   LE+                               
Sbjct: 635 ----------NGIMII--TLQVSFPELED------------------------------- 694

Query: 365 LTRLLFLKLNIQNVDILSNELSLDRAQSLEEFHIQVGNYVYSLRSDKFKTSLELETKSPI 424
                                                     LRS+     L LET    
Sbjct: 695 ------------------------------------------LRSE----GLSLET---- 754

Query: 425 GSIGGVLQIILQKCENLVVKDSVDFTKLFCNPNGNYGYPRLKHLQIIIREQDEDMPHGIR 484
                                                                       
Sbjct: 755 ------------------------------------------------------------ 814

Query: 485 NITPHFDLQLWHHNNGLTANSFCKLQRICINGCNKLTSVFPSHMVISLKFLNTLEVRYCK 544
                    LW HNNGL+ + FCKLQ I I  CNKL  VFPS++V SL FL  LE+  C+
Sbjct: 815 ---------LW-HNNGLSTHPFCKLQNIRIQYCNKLKYVFPSNVVTSLVFLKILEIYTCE 874

Query: 545 LVERIFEVEEQSNGVNQVMPMVSTKLNFLPNLKHMWNNDSARELLIFPNLKEVIIEECPQ 604
           L+E IFEVE+ ++     +P+    L  LP LK++W+ D   E L FPNL++V +  CP+
Sbjct: 875 LLEGIFEVEKLTDEARATVPLTKLILISLPKLKYVWDKDPG-EFLTFPNLRKVEVSGCPR 934

Query: 605 LKTLFPASFINHMKKIEKLYVDGENQIFAEDEASHLRKLTMHRKLVKEGSFWVISELFPK 664
           LK+  P SFI HM + + L  D                                      
Sbjct: 935 LKSFVPTSFIRHMDETKTLVTD-------------------------------------- 942

Query: 665 LHTFSLIGSKDDGTVSLPSEMSELVYRVKAIEIRNALELILMFQNGKENVKNSHQIFTSI 724
                              E S+LV            E++L    G EN+     +  S+
Sbjct: 995 -------------------EASKLV----------VPEMVLSKGFGDENI-----VTLSV 942

Query: 725 GLDKLPNFTHVWEDNNDQMMSRLKNIYVQGYDDLKCLVPSSITFLNLTSFMVINCKGMIN 784
              +  NF                 I V+  D + CL+PSSI+F NL S ++  C GMIN
Sbjct: 1055 ETSQESNF-----------------IQVKKCDKMNCLLPSSISFFNLNSLIIWECNGMIN 942

Query: 785 LFSSSMVKNLVNLKVMDITGCEGMISIVIAEEGGRQREDIIFNSLHILKLYKISRLTDFY 844
           LFSSS+ K+LV+L  + I+ CE M SI+  EE   + E+IIF  L  L+L  + RL +FY
Sbjct: 1115 LFSSSVAKSLVSLYCLTISNCEEMSSIIAREEEEEKDEEIIFKKLTDLELDDLPRLANFY 942

Query: 845 SGKYMLKFPCSEKLSIKQCSEMKTFSYGIINTPKLKHV 880
           SGKY LKFPC   L I +C +MK FSYGI    KL  V
Sbjct: 1175 SGKYSLKFPCLNNLRITKCPKMKPFSYGIEKQEKLNGV 942

BLAST of Lsi10G006250 vs. NCBI nr
Match: XP_038887230.1 (probable disease resistance protein At5g63020 [Benincasa hispida])

HSP 1 Score: 935.3 bits (2416), Expect = 4.2e-268
Identity = 539/981 (54.94%), Postives = 665/981 (67.79%), Query Frame = 0

Query: 2    NGYIQEIAKDVVKECGGLPIALKILAKALKGKRVEIWKDTCKKLKDPILVNIPGVSDQLY 61
            +GY+++IAK V+ ECGGLPIALKIL K LKG+RVEIWKD  KKLK+P++V+I GVSDQLY
Sbjct: 396  DGYMKQIAKYVINECGGLPIALKILGKTLKGRRVEIWKDALKKLKNPVVVDIHGVSDQLY 455

Query: 62   SCLQFSYDSIEDEAKLILFLCCVFPDDHRINLKDLQMYALSMRLLNGISAWRDARNRVIK 121
            SCLQFSYDSIE+EAKL+L LC VFPDDHRI + DLQ YA+ M LLN  + W DA+NRVIK
Sbjct: 456  SCLQFSYDSIENEAKLLLLLCSVFPDDHRIYVDDLQKYAMGMGLLNQTNTWEDAKNRVIK 515

Query: 122  LVDDLKSSSLLLKSDNGND-CVQMHDVVHDVAKYIASKIHKMPSLSYGSGESEWRDEDIC 181
            LVDDLKSS LLL     +D  V+MHDVVHD AKYIASKI KM S +YG+G+  W +++  
Sbjct: 516  LVDDLKSSYLLLAPPEWDDNYVKMHDVVHDFAKYIASKIDKMTSFTYGNGQRAWEEDNRH 575

Query: 182  GSYNAIYINCKKFLNFPPKLEFPNLQLFIL-SGVSDFRSAIRIPDTFFEGMKELRVLDVR 241
            GSYNAIY++   FLN PPKLEFPNLQLFIL S     R  I+  +T FEGMKELRVLDVR
Sbjct: 576  GSYNAIYVDPTTFLNLPPKLEFPNLQLFILISNGLGPRPTIQNLETVFEGMKELRVLDVR 635

Query: 242  TMCFAPSSWTSLTQSLKNLQTLCLTMREGSDFGTIGELKKLEILKIISSSFFQVLPASMS 301
               F P    SL  SLKNL+ LCL+  + +D   IGELK+L+IL+I      Q  P   S
Sbjct: 636  MWYFEP----SLIYSLKNLRVLCLSHCDSNDIDAIGELKRLKILEIHFCRGIQEFPECTS 695

Query: 302  ELTQLKVLHIL-CPGLKLIHPNILTSMRRLEELIVGCDYKRWGDEVTYKNKLIKNVKLSE 361
            +LTQLKVL +  C  LK+IHPNI++ M RLE+L     +  WG+EV YK KLI+N KLSE
Sbjct: 696  KLTQLKVLQVSHCMNLKVIHPNIISKMTRLEDLSFSHSFDGWGEEVWYKQKLIRNAKLSE 755

Query: 362  LDCLTRLLFLKL-NIQNVDILSNELSLDRAQSLEEFHIQVG-----------NYVYSLRS 421
            L+CLT L+ L+L N++NV+ILSNELSL+  Q LE   I VG           N  Y+ + 
Sbjct: 756  LNCLTHLVHLELCNLKNVEILSNELSLESTQKLETCRIFVGDERWNIHYNDRNNPYNRKD 815

Query: 422  DKFKTSLELETKSPIGSIGGVLQIILQKCENLVVKDSVDFTKLFCNPNGNYGYPRLKHLQ 481
             ++ T+L L   SPI SIGGV QI+LQKCE LV++DSV FT L CN   N  YP LKHL+
Sbjct: 816  KRYTTALNLYIDSPIDSIGGVFQILLQKCEKLVLRDSVGFTMLLCNTYANSCYPLLKHLE 875

Query: 482  IIIREQDEDMPHGIRNITP-HFDLQ-------------------LWHHNNGLTANSFCKL 541
            I      +  P  I NI+P +FDLQ                   LW  NNGLT N F KL
Sbjct: 876  I------DYPPAIIGNISPLNFDLQVAFPQLKSLYIGGRNNFETLW-RNNGLTENYFSKL 935

Query: 542  QRICINGCNKLTSVFPSHMVISLKFLNTLEVRYCKLVERIFEVEEQSN-GVNQVMPMVST 601
            +RI I GCNK T VFPS MV SL FLN L VRYC+LVERIFE+EE ++  VNQ +P+   
Sbjct: 936  ERIFIEGCNKFTYVFPSRMVRSLVFLNRLVVRYCELVERIFEIEESTSIIVNQDVPLTIL 995

Query: 602  KLNFLPNLKHMWNNDSARELLIFPNLKEVIIEECPQLKTLFPASFINHMKKIEKLYVDGE 661
            +L+ LPNLK++WNND A ELL FPNLK+V +EECPQLKTLFP SFINHMK+ E+L   GE
Sbjct: 996  ELSSLPNLKYVWNNDPALELLTFPNLKKVKVEECPQLKTLFPVSFINHMKEFEELDCVGE 1055

Query: 662  NQIFAE-DEASH---------LRKLTMHRKLVKEGSFWVISELFPKLHTFSLIGSKDDGT 721
             +IF E DEAS          LR LT+H  L+K  SFW   E F +L T  LIGS+DD  
Sbjct: 1056 TEIFGETDEASQLVSPQIFPSLRYLTVHYTLMKRRSFWFRPESFSELLTLRLIGSEDDDM 1115

Query: 722  VSLPSEMSE-LVYR--VKAIEIRNALELILMFQNGKENVKNSHQIFTS------IGLDKL 781
            VSLP EMSE  V+R  V+ ++I NA E + +F+NG+EN +N H   +       + L  L
Sbjct: 1116 VSLPFEMSESAVWRSVVRHLQIENAFEWVKIFRNGEENNQNYHASSSGGAKLKHLCLYNL 1175

Query: 782  PNFTHVWEDNNDQMMSR------LKNIYVQGYDDLKCLVPSSITFLNLTSFMVINCKGMI 841
            P  TH+WED+ DQM +       L++I V   D+LKCL+PSSITF NLT   V  C GM+
Sbjct: 1176 PKLTHIWEDSIDQMTATSCFVDILESIVVDRCDNLKCLLPSSITFSNLTFLDVERCNGMM 1235

Query: 842  NLFSSSMVKNLVNLKVMDITGCEGMISIVIAEEGGRQREDIIFNSLHILKLYKISRLTDF 901
            NLF SS+ KNLVNL+ M I  C  M SIV+  +G  + E+IIF+ L+ LKL  ++RL  F
Sbjct: 1236 NLFGSSVAKNLVNLREMWINECTEMRSIVVQPKGKEEGEEIIFDKLNELKLSNLTRLASF 1295

Query: 902  YSGKYMLKFPCSEKLSIKQCSEMKTFSYGIINTPKLKHVEIQEGTQARGTVLVSQARGIN 922
            +S K +LKFP  EKL I  C EMKTFSYGII+TPKL HVE  EG +    V VS A+G+N
Sbjct: 1296 HSEKCVLKFPSLEKLKISGCPEMKTFSYGIISTPKLSHVE-GEGNK----VSVSLAQGLN 1355

BLAST of Lsi10G006250 vs. NCBI nr
Match: XP_022143564.1 (probable disease resistance protein At4g27220 [Momordica charantia])

HSP 1 Score: 777.3 bits (2006), Expect = 1.5e-220
Identity = 450/889 (50.62%), Postives = 575/889 (64.68%), Query Frame = 0

Query: 5    IQEIAKDVVKECGGLPIALKILAKALKGKRVEIWKDTCKKLKDPILVNIPGVSDQLYSCL 64
            I+ IAK+VVK+CGGLPIALKI+AKALKGK + IWKD  +KL+  +LV I GVSDQLYSCL
Sbjct: 335  IEHIAKNVVKKCGGLPIALKIIAKALKGKEIHIWKDAFEKLEKHVLVKIKGVSDQLYSCL 394

Query: 65   QFSYDSIED-EAKLILFLCCVFPDDHRINLKDLQMYALSMRLLNGISAWRDARNRVIKLV 124
            + SYD IED E +L+  LC VFPDDH I +KDLQMYA+ M LLN I+ W   +NRVI LV
Sbjct: 395  KLSYDWIEDEETRLLFLLCSVFPDDHNICVKDLQMYAMGMGLLNDINNWEATKNRVIDLV 454

Query: 125  DDLKSSSLLLKSDNGNDCVQMHDVVHDVAKYIASKIHKMPSLSYGSGESEWRDEDICGSY 184
            DDLKSS LLL+S++G++ VQMHDVV DVAKYIAS   KM SLSYG G+SEW +ED  GSY
Sbjct: 455  DDLKSSYLLLESNSGDNYVQMHDVVRDVAKYIASNDDKMSSLSYGCGQSEWLEEDRSGSY 514

Query: 185  NAIYINCKKFLNFPPKLEFPNLQLFILSGVSDF--RSAIRIPDTFFEGMKELRVLDVRTM 244
            NAI+++C  F + P   EFPNLQL IL   S F    AI+IP  FFEGM++LRVLD+  M
Sbjct: 515  NAIFVDCMDFSSLPSNFEFPNLQLLILGMPSFFGEGEAIQIPGAFFEGMEKLRVLDMTKM 574

Query: 245  CFAPSSWTSLTQSLKNLQTLCLTMREGSDFGTIGELKKLEILKIISSSFFQVLPASMSEL 304
            CF PS  TS+T SLKNLQTLC++  E  D  TIG+LKKL+ILK I+   F+ LPA+MS+L
Sbjct: 575  CFEPSR-TSVTHSLKNLQTLCMSYCEYYDIDTIGQLKKLKILK-INDCTFEELPANMSQL 634

Query: 305  TQLKVLHIL-CPGLKLIHPNILTSMRRLEELIVGCDYKRWGDEVTYKNKLIKNVKLSELD 364
            TQL++L +  C  LK+I  NI++SM +LEEL +   +KRWG+E++Y+NKLI+NVKLSEL+
Sbjct: 635  TQLRLLDLSNCSNLKVIPINIISSMTKLEELNLWRSFKRWGEEISYRNKLIQNVKLSELN 694

Query: 365  CLTRLLFLKLNIQNVDILSNELSLDRAQSLEEFHIQVGNYVYSLR-SDKFKTSLELETKS 424
             L+RL  L+L I NV+I S++LSL+R + LEEF I VGN  +  R  D +   L +  KS
Sbjct: 695  HLSRLSNLRLEIPNVNIFSDDLSLERVEKLEEFCICVGNDSWGFRICDGYARRLMVNIKS 754

Query: 425  PIGSIGGVLQIILQKCENLVVKDSVDFTKLFCNPNGNYGYPRLKHLQIIIREQDEDMPHG 484
             I +IGG LQI+L+ CE L ++D+V FT      NGNY  PRLKHL I   E + +MP  
Sbjct: 755  QIVAIGGTLQILLEGCEQLYIEDTVGFTNCLFKLNGNYCNPRLKHLHI---EGNSEMPRR 814

Query: 485  I-----------RNITPHFDLQLWHHNNGLTANSFCKLQRICINGCNKLTSVFPSHMVIS 544
            I             I    +L++  HNN L ANSFCKL+ ICI+ CNKLT +F S+MV S
Sbjct: 815  IDVQVSFPELKELTIQGGNNLEMLWHNNRLIANSFCKLRSICISCCNKLTYMFTSNMVTS 874

Query: 545  LKFLNTLEVRYCKLVERIFEVEEQSNGVNQVMPMVSTKLNFLPNLKHMWNNDSARELLIF 604
            L FLNTLE+  C+L+ERIFE+ +  +   +V+P+    L  LPNLK++WNND   E L F
Sbjct: 875  LVFLNTLEIDNCELLERIFEIGKLVDDA-KVLPLTDLSLRSLPNLKYVWNNDPG-EFLTF 934

Query: 605  PNLKEVIIEECPQLKTLFPASFINHMKKIEKLYVDGENQIFAEDEASHL-------RKLT 664
            PNLK V +  CP+LK LFPASFI H+++IE L     ++IF+EDEAS L       R L 
Sbjct: 935  PNLKNVNVSSCPRLKCLFPASFIKHIEEIESLCAYEVDEIFSEDEASKLGFPEIVFRSLQ 994

Query: 665  MHRKLVKEGSFWVISELFPKLHTFSLIGSKDDGTVSLPSEMSELVYRVKAIEIRNALELI 724
            +   +VK  SFW  S  FPKLH   L G +DD  V+LP EMS+ +Y              
Sbjct: 995  ILEMVVKR-SFWFRSGSFPKLHNLKLKGGEDDDLVTLPLEMSQELY-------------- 1054

Query: 725  LMFQNGKENVKNSHQIFTSIGLDKLPNFTHVWEDNNDQMMSRLKNIYVQGYDDLKCLVPS 784
                                                        NI V+  D LK L+PS
Sbjct: 1055 --------------------------------------------NIEVKKCDKLKFLLPS 1114

Query: 785  SITFLNLTSFMVINCKGMINLFSSSMVKNLVNLKVMDITGCEGMISIVIAEEGGRQREDI 844
            S TF NL S ++ +C GM+NLF++ + KNLVNL ++ I  C G+ SIV  E    + E+I
Sbjct: 1115 STTFFNLGSLIIEDCNGMMNLFNTLVAKNLVNLCLIKICDCRGITSIVAEEVEEEEGEEI 1157

Query: 845  IFNSLHILKLYKISRLTDFYSGKYMLKFPCSEKLSIKQCSEMKTFSYGI 871
            IFN L  L+L  + RL +FYSGK  LKFP  E+L I+ C +MKTFS+GI
Sbjct: 1175 IFNHLIRLELRDLPRLANFYSGKCALKFPYLERLIIEGCPKMKTFSFGI 1157

BLAST of Lsi10G006250 vs. NCBI nr
Match: XP_038890209.1 (probable disease resistance protein At4g27220 [Benincasa hispida])

HSP 1 Score: 669.8 bits (1727), Expect = 3.3e-188
Identity = 421/1059 (39.75%), Postives = 603/1059 (56.94%), Query Frame = 0

Query: 2    NGYIQEIAKDVVKECGGLPIALKILAKALKGKRVEIWKDTCKKLKDPILVNIPGVSDQLY 61
            N  ++ I K++V++CGGLPIAL  + KALKGK   IWKD  K+LK+PI V++ GVSD +Y
Sbjct: 285  NKCVELIGKEMVRKCGGLPIALATIVKALKGKEEAIWKDALKQLKNPITVDVKGVSDVVY 344

Query: 62   SCLQFSYDSIE-DEAKLILFLCCVFPDDHRINLKDLQMYALSMRLLNGISAWRDARNRVI 121
            + L+ SYD ++ +EA+L+  LC VFPDD+ I+++DLQ+YA+ +RLLN ++ W +ARNRVI
Sbjct: 345  ASLKLSYDYLKGEEARLLFLLCSVFPDDYEISVEDLQIYAMGLRLLNQVNTWDEARNRVI 404

Query: 122  KLVDDLKSSSLLLKSDNGNDCVQMHDVVHDVAKYIASKIHKMPSLSYGSGESEWRDEDIC 181
            KLVDDLK+SSLLL+S++ ++ V+MHD V DVA YIASK   M +LSYG G SEW+++D  
Sbjct: 405  KLVDDLKASSLLLESNSRDNHVKMHDTVRDVAIYIASKEANMSTLSYGFGLSEWQEKDRH 464

Query: 182  GSYNAIYINCKKFLNFPPKLEFPNLQLFILSGVSDFRSAIRIPDTFFEGMKELRVLDVRT 241
            GSY AI+ NC  F N P  LEFP L+L IL G       ++I D FFEGMKEL+VL++  
Sbjct: 465  GSYRAIFGNCHNFYNLPQNLEFPKLELLILDGHDWRGEKLQICDPFFEGMKELKVLNLSR 524

Query: 242  MCFAPSSWTSLTQSLKNLQTLCLTMREGSDFGTIGELKKLEILKIISSSFFQVLPASMSE 301
            M F  + W S   SL+NLQTLC++    +D   I  LKKL+IL++   S   +LP SMS+
Sbjct: 525  MSF-QTLWRSSIYSLENLQTLCMSYCTFNDIDAISHLKKLQILRVDKCS-ITLLPISMSQ 584

Query: 302  LTQLKVLHILCPGLKLIHPNILTSMRRLEELIVGCDYKRWGDEVTYKNKLIKNVKLSELD 361
            LTQLKVL +    LK+I PNI++SM +L+ L +   +  WG+EV Y NKLI N + SEL 
Sbjct: 585  LTQLKVLQVSNCPLKVIPPNIISSMLKLQALDICTSFNGWGEEVLYNNKLINNARPSELK 644

Query: 362  CLTRLLFLKLNIQNVDILSNELSLDRAQSLEEFHIQVGNYVYSLR---SDKFKTSLELE- 421
            CL+ L  LKL+I +++IL+ +L   +   LE F I +G+   S R    +++ T+L L+ 
Sbjct: 645  CLSHLTNLKLHILDINILA-DLLFFKNLKLERFVIHIGDLKMSQRLQGCEQYATTLMLKI 704

Query: 422  --TKSPIGSIG--GVLQIILQKCENLVVKDSVDFTKLFCNPNGNYGYPRLKHLQII---- 481
              + S I SI    VL+I+L++ ENL V+ S+  T +   PN    YP L+HL +     
Sbjct: 705  ITSSSQIVSIDHHEVLEILLKQSENLCVEGSMMVTNIHFKPNNGNNYPYLRHLTLTKDSK 764

Query: 482  ----------------------IREQDEDMPHGIRNITPH-------------------- 541
                                    E  E++ H   +  P                     
Sbjct: 765  LRHLIGNGCFAYFPSLEFLSLEKMESLENIVHVQVSTNPFRKLRSIKVISCKRLRYLFSF 824

Query: 542  ------FDLQ------------------------------------------------LW 601
                   DLQ                                                LW
Sbjct: 825  SIFKGLVDLQKVFIYDCNMMDEIFFMDSEDSTIKAECNCVAFPQLKDLTIIGANNLKTLW 884

Query: 602  HHNNGLTANSFCKLQRICINGCNKLTSVFPSHMVISLKFLNTLEVRYCKLVERIFEVEEQ 661
             H NGL  N F KLQRI INGCN L  +FPS++V++L  LNTL V  C L++ IFE+E  
Sbjct: 885  -HKNGLAPNFFTKLQRISINGCNNLRYIFPSNVVVALVSLNTLVVESCYLLDGIFEMENT 944

Query: 662  SNGVN---------QVMPMVSTKLNFLPNLKHMWNNDSARELLIFPNLKEVIIEECPQLK 721
            SN            QV+P+V   L  LPNLK++WN D  +  L FP L++VI   CPQL+
Sbjct: 945  SNFKQKDDDDDDDAQVIPLVEVNLKHLPNLKYVWNKDVGK-FLKFPRLRDVIAINCPQLQ 1004

Query: 722  TLFPASFINHMKKIEKLYVDGENQIFAEDEAS----------HLRKLTMHRKLVKEGSFW 781
            TLFPAS I H+ +++ L V G +++F+EDE S           L  L+M  K +   +FW
Sbjct: 1005 TLFPASLIKHVHQLQNLVVLGIHKMFSEDETSKSMFHEILFPSLTGLSMDTKAMTT-NFW 1064

Query: 782  VI----SELFPKLHTFSLIGSKDDGTVSLPSEMSELVYRVKAIEIRNALELILMFQNGKE 841
            +     S  FP L   SL+GS DD   +LP EM++++Y +K + +    +L+ +F N +E
Sbjct: 1065 LTQFSKSRSFPNLRNLSLMGSYDDEMTTLPLEMTQILYNIKVLNVSFVSQLVQIFSN-EE 1124

Query: 842  NVKNSHQ---IFTSIGLDKLPNFTHVWEDNNDQ--MMSRLKNIYVQGYDDLKCLV-PSSI 901
             + N  Q       + L  LPN  HVW+DN     +   L+ I+V+    LKCLV PSSI
Sbjct: 1125 EISNPIQRCADLKKLTLSHLPNLKHVWKDNIQMTILFDNLEAIHVEECGKLKCLVPPSSI 1184

Query: 902  TFLNLTSFMVINCKGMINLFSSSMVKNLVNLKVMDITGCEGMISIVIAEEGGRQREDIIF 922
            TF NL    V  C  ++NL   S+ K+L  L+ + I+ C+GM SI+   EG   +++I+F
Sbjct: 1185 TFSNLKYLSVDRCHELMNLLCFSIAKSLSKLEKLRISECQGMSSILAKGEGEEGKDEIVF 1244

BLAST of Lsi10G006250 vs. NCBI nr
Match: XP_038900638.1 (LOW QUALITY PROTEIN: probable disease resistance protein At4g27220 [Benincasa hispida])

HSP 1 Score: 619.4 bits (1596), Expect = 5.1e-173
Identity = 406/1062 (38.23%), Postives = 558/1062 (52.54%), Query Frame = 0

Query: 4    YIQEIAKDVVKECGGLPIALKILAKALKGKRVEIWKDTCKKLKDPILVNIPGVSDQLYSC 63
            Y++ IAK+VV+ECGGLP+AL  +AKALK KR+  W+D   KLK    V I GV+ ++Y+ 
Sbjct: 324  YMEIIAKEVVRECGGLPLALDTIAKALKEKRINYWEDALSKLKTSNAVGIKGVTHKVYAS 383

Query: 64   LQFSYDSIE-DEAKLILFLCCVFPDDHRINLKDLQMYALSMRLLNGISAWRDARNRVIKL 123
            L+ SYD ++ DE+K++  LC VFPDD+ I+ KDLQ+YA+ MRLL  ++ W +A+N +IKL
Sbjct: 384  LRLSYDYLDGDESKILFLLCSVFPDDYMISTKDLQVYAMGMRLLRRVNTWGEAKNSIIKL 443

Query: 124  VDDLKSSSLL----LKSDNGNDCVQMHDVVHDVAKYIASKIHKMPSLSYGSGESEWRDED 183
            VDDL SS LL     K    +  V+MHD+V DVA YIAS    M +L YGS  SEW D D
Sbjct: 444  VDDLTSSXLLQVQVYKDTRYDQYVKMHDIVRDVAIYIASTTTNMSTLYYGSKHSEWPDTD 503

Query: 184  ICGSYNAIYINCKKFLNFPPKLEFPNLQLFILSGVSDFRS-AIRIPDTFFEGMKELRVLD 243
             CGSY AI+I+CK   NF P ++FP L+L IL   +      I   D FFEGMK L+VLD
Sbjct: 504  NCGSYRAIFIDCKNICNFLPSMKFPKLELLILRFSTKLSEVCIETIDAFFEGMKNLKVLD 563

Query: 244  VRTMCFAPSSWTSLTQSLKNLQTLCLTMREGSDFGTIGELKKLEILKIISSSFFQVLPAS 303
            + T    PS    L  SLK L+TLCL+  E  D   I  LK+LEIL+IIS      LPA+
Sbjct: 564  L-TKIHVPS---VLWSSLKKLRTLCLSQCECKDIDKISHLKELEILRIISCEGITELPAT 623

Query: 304  MSELTQLKVLHI-LCPGLKLIHPNILTSMRRLEELIVGCDYKRWGDEVTYKNKLIKNVKL 363
            MSEL QLKVL +  C  L +IHPNI++S+ +LEEL +   +++WG++V YKN  I+N +L
Sbjct: 624  MSELKQLKVLVVSKCYNLVVIHPNIISSLTKLEELDIQLCFQKWGEKVRYKNTWIQNARL 683

Query: 364  SELDCLTRLLFLKLNIQNVDILSNELSLDRAQSLEEFHIQVGNYVYSL-----RSDKFKT 423
             E++ L  L  LKL I   ++   +L     + L EF I VG   +        SDK++ 
Sbjct: 684  LEVNYLPHLSILKLQISKANVGLEDLGSQTLKKLREFFICVGTDHFKFESWKNNSDKYEK 743

Query: 424  SLELETKSPIGSIGG-VLQIILQKCENL-VVKDSVDFTKLFCNPNGNYGYPRLKHLQIII 483
            +  L  +  +  + G V   + +  E L +V DS  F  +    NGN GYP LK L I  
Sbjct: 744  TFLLNMELQMSLVDGMVFDKLFEGTEQLKIVNDSKGFANVILKANGN-GYPLLKCLMI-- 803

Query: 484  REQDEDMPHGIRN----------------------------------------------- 543
             + +  M H I N                                               
Sbjct: 804  -DGNSKMSHLIGNDFTSLECLSLSEMMLLESIVPKHASTNPFNKLIVIQIGSCKQLRNLF 863

Query: 544  ------------------------------------------------------------ 603
                                                                        
Sbjct: 864  SFSIFKGLSNLQEIKVTYCDMMDEIVSVVEIEDQTTICTFPLTTLHLEGLNKVTSFCTKP 923

Query: 604  --------ITPHFDLQ-------------------LWHHNNGLTANSFCKLQRICINGCN 663
                    I P FD Q                   LW HN+G TA+SFCKL+ I I  CN
Sbjct: 924  FIQQRPQTIIPFFDQQVSFPEMKNLTIKGGNNLEMLW-HNDGPTASSFCKLRSISIGNCN 983

Query: 664  KLTSVFPSHMVISLKFLNTLEVRYCKLVERIFEVEEQSNGVNQVMPMVSTKLNFLPNLKH 723
            KL  +FP ++V SL  L+TL +  C+L+E IFE+E+ S G    M +    LN LPNLK+
Sbjct: 984  KLRYIFPWNIVTSLALLDTLFIFSCELLESIFEIEKPSVGDTIAMSLRKVDLNGLPNLKY 1043

Query: 724  MWNNDSARELLIFPNLKEVIIEECPQLKTLFPASFINHMKKIEKLYVDGENQIFAEDEA- 783
            MWN D   +++ FP LKEV++ +CP++K++FPASF  +++ +E+L VD +N+IF  D+A 
Sbjct: 1044 MWNRDVC-DVMAFPKLKEVLVTDCPEMKSIFPASFPQYIEAVERLEVDEQNEIFPVDKAL 1103

Query: 784  --------SHLRKLTMHRKLVKEGSFWVISELFPKLHTFSLIGSKD-DGTVSLPSEMSEL 843
                     +LR L M  KL  + SFWVIS+ F KL + +L+G ++ D  VSLP EMSE+
Sbjct: 1104 KLGEVVLFRNLRSLKMTCKLATKESFWVISK-FLKLESITLVGYEEGDKMVSLPLEMSEV 1163

Query: 844  VYRVKAIEIRNALELILMFQNGKE--NVKNSHQIFTSIGLDKLPNFTHVWEDNNDQMMSR 903
            +Y ++ ++I    +L+ +F N  E  N++        + L  LP  T+VW++ N      
Sbjct: 1164 LYNIEELKIETCFQLVYIFGNETENDNIQRRCAHLKKLKLKNLPKLTNVWKNVNQM---- 1223

Query: 904  LKNIYVQGYDDLKCLVPSSITFLNLTSFMVINCKGMINLFSSSMVKNLVNLKVMDITGCE 906
                             ++ITF NL    V NC GM++LFS S+ KNLVNL+ + I  C 
Sbjct: 1224 -----------------TTITFFNLADLDVSNCNGMMSLFSYSVAKNLVNLERIKIYDCR 1283

BLAST of Lsi10G006250 vs. NCBI nr
Match: XP_038902150.1 (disease resistance protein At4g27190-like [Benincasa hispida])

HSP 1 Score: 552.7 bits (1423), Expect = 5.8e-153
Identity = 364/941 (38.68%), Postives = 534/941 (56.75%), Query Frame = 0

Query: 4    YIQEIAKDVVKECGGLPIALKILAKALKGKRVEIWKDTCKKLKDPILVNIPGVSDQLYSC 63
            Y++ IAK+V KECGGLP+AL  +AKALKGK +  W+D   KLK+ I ++I GVSD++Y+ 
Sbjct: 317  YMESIAKEVSKECGGLPLALDTIAKALKGKDMHYWEDALSKLKNSIGMDIKGVSDKVYAS 376

Query: 64   LQFSYDSIE-DEAKLILFLCCVFPDDHRINLKDLQMYALSMRLLNGISAWRDARNRVIKL 123
            L+ SY+ ++ +E KL+  LC VFPDD++I +KDLQMYA+ MRLLN +  W DA+NRV+KL
Sbjct: 377  LRLSYEYLDGEETKLLFLLCSVFPDDYKIGMKDLQMYAMGMRLLNKVKTWEDAKNRVMKL 436

Query: 124  VDDLKSSSLLLK--SDNGNDCVQMHDVVHDVAKYIASKIHKMPSLSYGSGESEWRDEDIC 183
            V DL SSSL L+  SD+ +  V+MHDVV DVA +IAS    M +L+ G   +EW DE   
Sbjct: 437  VHDLISSSLFLEADSDSKDKYVKMHDVVRDVAIHIASNEGNMSTLNIGYKLNEWEDEYRS 496

Query: 184  GSYNAIYINCKKFLNFPPKLEFPNLQLFILSGVSDF--RSAIRIPDTFFEGMKELRVLDV 243
            GS+ AI+ NC    N PPK++FP L+L IL  VS++   + ++IP  FFEGM++L+VLD+
Sbjct: 497  GSHRAIFANCDNLNNLPPKMKFPQLELLILR-VSNWLVENNLQIPYAFFEGMEKLKVLDM 556

Query: 244  RTMCFAPSSWTSLTQSLKNLQTLCLTMREGSDFGTIGELKKLEILKIISSSFFQVLPASM 303
              MCF    WT  T SL NL+TLC+   E +D  TIGELKKLEIL+I+  +    LP +M
Sbjct: 557  TGMCFHRPLWT--TSSLNNLRTLCMVCCEFNDIDTIGELKKLEILRIMKCNMLDHLPTTM 616

Query: 304  SELTQLKVLHIL-CPGLKLIHPNILTSMRRLEELIVGCDYKRWGDEVTYKNKLIKNVKLS 363
            S+LT LKVL +L C  L+++  NI +SM +LEEL +   + RWG+EV YK++LIKNVKLS
Sbjct: 617  SQLTHLKVLEVLNCSKLEVVPANIFSSMTKLEELKLQDSFCRWGEEVWYKDELIKNVKLS 676

Query: 364  ELDCLTRLLFLKLNIQNVDILSNELSLDRAQSLEEFHI---QVGNYVYSLRSDKFKTSLE 423
            EL+ L RL  L L    V ILS E+S    ++L+EF I   +  + +    S+++ T+L 
Sbjct: 677  ELNYLPRLSNLCLESWKVKILS-EISSQTCKNLKEFWICSNESDDIIQPKVSNEYATTLM 736

Query: 424  LETKSPIGSIGGVLQIILQKCENLVVKDSV-DFTKLFCNPNGNYGYPRLKHLQIIIREQD 483
            L  +S IGSI G L+I+LQ+ E L+V DS+ +F      PNGN G+P LK+L II    +
Sbjct: 737  LNIESQIGSIDGGLEILLQRSERLIVSDSMGNFVNGIFKPNGN-GFPLLKYLWIIDEHSN 796

Query: 484  EDMPHGIRNITPHFDLQLWHHNNGL--------TANSFCKLQRICINGCNKLTSVFPSHM 543
             +MPH I +        + +    L        T + F KL+ I I  C +L ++F   +
Sbjct: 797  SEMPHLIGSDFTSLKYLILYGMRRLENIVPKHITISPFKKLKTIAIQFCGQLRNLFSFSI 856

Query: 544  VISLKFLNTLEVRYCKLVERI--FEVEEQSN-----------------------GVNQVM 603
               L  L  +EV  C +++ I   E+E+Q N                       G+ Q  
Sbjct: 857  FKGLSDLQEIEVINCGMMDEIIFMEIEDQPNICTTPLASLILENVDKLSSFCTKGLIQES 916

Query: 604  P----------MVSTKLNFL-----PNLKHMWNNDSARELLIFPNLKEVIIEECPQLKTL 663
            P          ++  +LN L      NL+ +W+ ++      F  L+ + IE C +++ +
Sbjct: 917  PQSILPFFDQQVLFPELNDLSIIGSKNLETLWHKNNEPTTNSFCKLQSIRIEMCTKIRCM 976

Query: 664  FPASFINHMKKIEKLYVDGENQIFAEDEASHLRKLTMHRKLVKEGSF-WVISELFPKLHT 723
            FP++ +  +  ++ + +           AS  R   + ++   +    W + E    LH 
Sbjct: 977  FPSNMVTSLVSLDTIEI--------TSCASLKRIFEIEKESFGDTKVAWSLRE----LHL 1036

Query: 724  FSLIGSKDDGTVSLPSEMSELVYRVKAIEIRNALELILMFQNGKENVKNSHQIFTSIGLD 783
             +L   K              V+R   IE        + F + K            + + 
Sbjct: 1037 LNLPNLKH-------------VWRKDVIE-------FMKFPSLKR-----------VKIH 1096

Query: 784  KLPNFTHVWEDNNDQMMS--RLKNIYVQGYDDLKCLVPSSITFLNLTSFMVINCKGMINL 843
                  H+W++N     S   L++I V+  ++LK L+PSSITFLNL    V  C GM+NL
Sbjct: 1097 GCTKLAHIWKENTKMTTSFDSLESIEVERCENLKYLLPSSITFLNLNELHVKECNGMMNL 1156

Query: 844  FSSSMVKNLVNLKVMDITGCEGMISIVIAEEGGRQREDIIFNSLHILKLYKISRLTDFYS 884
            FSS++ KNLVNL+ ++++ CEGM  IV A+E     E +IF  L  L+   + RL  FYS
Sbjct: 1157 FSSTVAKNLVNLRSIEVSYCEGMSCIVEAKE--ENDESVIFKKLGKLEFDCLPRLDSFYS 1207

BLAST of Lsi10G006250 vs. TAIR 10
Match: AT4G27220.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 147.9 bits (372), Expect = 4.0e-35
Identity = 163/637 (25.59%), Postives = 287/637 (45.05%), Query Frame = 0

Query: 5   IQEIAKDVVKECGGLPIALKILAKALKGK-RVEIWKDTCKKLKDPILVNIPGV--SDQLY 64
           ++ IAKDV  EC GLP+A+  + + L+GK +VE+WK T   LK     + P +   ++++
Sbjct: 294 VKPIAKDVSHECCGLPLAIITIGRTLRGKPQVEVWKHTLNLLKR----SAPSIDTEEKIF 353

Query: 65  SCLQFSYDSIEDEAKLILFLCCVFPDDHRINLKDLQMYALSMRLLNGISAWRDARNRVIK 124
             L+ SYD ++D  K     C +FP+D+ I + +L MY ++  LL+G   + D  N  + 
Sbjct: 354 GTLKLSYDFLQDNMKSCFLFCALFPEDYSIKVSELIMYWVAEGLLDGQHHYEDMMNEGVT 413

Query: 125 LVDDLKSSSLLLKSDNGNDCVQMHDVVHDVAKYIASK----IHKMPSLSYGSGESEWRDE 184
           LV+ LK S LL   D+  D V+MHDVV D A +  S      H +  +  G G  E+  +
Sbjct: 414 LVERLKDSCLLEDGDS-CDTVKMHDVVRDFAIWFMSSQGEGFHSL--VMAGRGLIEFPQD 473

Query: 185 DICGSYNAIYINCKKFLNFPPK-LEFPNLQLFILSGVSDFRSAIRIPDTFFEGMKELRVL 244
               S   + +   K    P   +E     + +L G S  +    +P+ F +    LR+L
Sbjct: 474 KFVSSVQRVSLMANKLERLPNNVIEGVETLVLLLQGNSHVK---EVPNGFLQAFPNLRIL 533

Query: 245 D-----VRTMCFAPSSWTSLTQSLKNLQTLCLTMREGSDFGTIGELKKLEILKIISSSFF 304
           D     +RT+   P S+++L  SL++L  +    ++  +  ++  L KL+ L +  S+  
Sbjct: 534 DLSGVRIRTL---PDSFSNL-HSLRSL--VLRNCKKLRNLPSLESLVKLQFLDLHESA-I 593

Query: 305 QVLPASMSELTQLKVLHIL-CPGLKLIHPNILTSMRRLEELIVGCDYKRWGDEVTYKNKL 364
           + LP  +  L+ L+ + +     L+ I    +  +  LE L +      WG +   +   
Sbjct: 594 RELPRGLEALSSLRYICVSNTYQLQSIPAGTILQLSSLEVLDMAGSAYSWGIKGEERE-- 653

Query: 365 IKNVKLSELDCLTRLLFLKLNIQNVDILSNELSLDR-AQSLEEFHIQVGNYVYS-LRSDK 424
                L E+ CL  L FL + +  +D+LS     D   + L +F      +++S +RS  
Sbjct: 654 -GQATLDEVTCLPHLQFLAIKL--LDVLSFSYEFDSLTKRLTKF-----QFLFSPIRSVS 713

Query: 425 FKTSLE-----LETKSPIGSIGGVLQII----LQKC-------ENLVVKDSVDFTKLFCN 484
              + E      +      SIG +LQ +    L  C       ENLV K    F  +   
Sbjct: 714 PPGTGEGCLAISDVNVSNASIGWLLQHVTSLDLNYCEGLNGMFENLVTKSKSSFVAM--K 773

Query: 485 PNGNYGYPRLKHLQIIIREQDEDMPHGIRNITPHFDLQLWHHNNGLTANSFCKLQRICIN 544
               + +P L  L      Q +  P+       + +L+     NG       KL+ + ++
Sbjct: 774 ALSIHYFPSLS-LASGCESQLDLFPNLEELSLDNVNLESIGELNGFLGMRLQKLKLLQVS 833

Query: 545 GCNKLTSVFPSHMVI-SLKFLNTLEVRYCKLVERIFEVEEQSNG--VNQVMPMVST-KLN 604
           GC +L  +F   ++  +L  L  ++V  C  +E +F             ++P ++  KL 
Sbjct: 834 GCRQLKRLFSDQILAGTLPNLQEIKVVSCLRLEELFNFSSVPVDFCAESLLPKLTVIKLK 893

Query: 605 FLPNLKHMWNNDSARELLIFPNLKEVIIEECPQLKTL 606
           +LP L+ + N     + ++  +L+ + +E C  LK L
Sbjct: 894 YLPQLRSLCN-----DRVVLESLEHLEVESCESLKNL 895

BLAST of Lsi10G006250 vs. TAIR 10
Match: AT4G27190.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 147.1 bits (370), Expect = 6.9e-35
Identity = 158/651 (24.27%), Postives = 287/651 (44.09%), Query Frame = 0

Query: 4   YIQEIAKDVVKECGGLPIALKILAKALKGKR-VEIWKDTCKKLKDPILVNIPGVSDQLYS 63
           ++++IAK V +ECGGLP+A+  +  A++GK+ V++W     KL   +   I  + ++++ 
Sbjct: 323 HVRKIAKAVSQECGGLPLAIITVGTAMRGKKNVKLWNHVLSKLSKSV-PWIKSIEEKIFQ 382

Query: 64  CLQFSYDSIEDEAKLILFLCCVFPDDHRINLKDLQMYALSMRLLNGISAWRDARNRVIKL 123
            L+ SYD +ED+AK    LC +FP+D+ I + ++  Y ++   +  + +  D+ N  I  
Sbjct: 383 PLKLSYDFLEDKAKFCFLLCALFPEDYSIEVTEVVRYWMAEGFMEELGSQEDSMNEGITT 442

Query: 124 VDDLKSSSLLLKSDNGNDCVQMHDVVHDVAKYI--ASKIHKMPSLSYGSGESEWRDEDIC 183
           V+ LK   LL   D   D V+MHDVV D A +I  +S+      +  G+G  + R + + 
Sbjct: 443 VESLKDYCLLEDGDR-RDTVKMHDVVRDFAIWIMSSSQDDSHSLVMSGTGLQDIRQDKLA 502

Query: 184 GSYNAIYINCKKFLNFPPKLEFPNLQLFILSGVSDFRSAIRIPDTFFEGMKELRVLDV-- 243
            S   + +   K  + P  +E   ++  +L    +F     +P  F +    LR+L++  
Sbjct: 503 PSLRRVSLMNNKLESLPDLVEEFCVKTSVLLLQGNFLLK-EVPIGFLQAFPTLRILNLSG 562

Query: 244 -RTMCFAPSSWTSLTQSLKNLQTLCLTMREGSDFGTIGELKKLEILKIISSSFFQVLPAS 303
            R   F   S   L          C  + +     ++  L KLE+L +  +   +  P  
Sbjct: 563 TRIKSFPSCSLLRLFSLHSLFLRDCFKLVK---LPSLETLAKLELLDLCGTHILE-FPRG 622

Query: 304 MSELTQLKVLHI-LCPGLKLIHPNILTSMRRLEELIVGCDYKRWGDE-VTYKNKLIKNVK 363
           + EL + + L +     L+ I   +++ +  LE L +   + RW  +  T K +      
Sbjct: 623 LEELKRFRHLDLSRTLHLESIPARVVSRLSSLETLDMTSSHYRWSVQGETQKGQ----AT 682

Query: 364 LSELDCLTRLLFLKLNIQNVDILSNELSLDRAQSLEEFHIQVGN-YVYSLRSDKFKTSLE 423
           + E+ CL RL  L + + +   L N+ +    + L++F + VG+ Y+   R DK + ++ 
Sbjct: 683 VEEIGCLQRLQVLSIRLHSSPFLLNKRN-TWIKRLKKFQLVVGSRYILRTRHDKRRLTIS 742

Query: 424 LETKSPIGSIGGVL----QIILQKCENLVVKDSVDFTKLFCNPNGNYGYPRLKHLQI--- 483
               S + SIG +L     + L  C+ +         KL    + N G+  LK L I   
Sbjct: 743 HLNVSQV-SIGWLLAYTTSLALNHCQGI----EAMMKKLV---SDNKGFKNLKSLTIENV 802

Query: 484 ---------IIREQDEDMPHGIRNITPHFDLQLWHHNNGLTANSFCKLQR---------- 543
                    ++          I ++ P+ + +L  H   +   +F +LQ           
Sbjct: 803 IINTNSWVEMVSTNTSKQSSDILDLLPNLE-EL--HLRRVDLETFSELQTHLGLKLETLK 862

Query: 544 -ICINGCNKLTSVFPSHMVISLKFLNTLEVRYCKLVERIFEVEEQSNGVNQVMPMVSTKL 603
            I I  C KL ++      +++  L  +E+ YC  ++ + E          V  +   KL
Sbjct: 863 IIEITMCRKLRTLLDKRNFLTIPNLEEIEISYCDSLQNLHEALLYHQPF--VPNLRVLKL 922

Query: 604 NFLPNLKHMWNNDSARELLIFPNLKEVIIEECPQLKTLFPASFINHMKKIE 619
             LPNL  + N     E      L++V +  C QL  L  +S    +KKI+
Sbjct: 923 RNLPNLVSICNWGEVWEC-----LEQVEVIHCNQLNCLPISSTCGRIKKIK 943

BLAST of Lsi10G006250 vs. TAIR 10
Match: AT5G63020.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 95.5 bits (236), Expect = 2.4e-19
Identity = 156/618 (25.24%), Postives = 260/618 (42.07%), Query Frame = 0

Query: 5   IQEIAKDVVKECGGLPIALKILAKALKGKR-VEIWKDTCKKLKDPILVNIPGVSDQLYSC 64
           I  +A+ V K+C GLP+AL ++ + +  KR V+ W+     L         G+ D++   
Sbjct: 335 IPTVARTVAKKCRGLPLALNVIGETMAYKRTVQEWRSAIDVLTSS-AAEFSGMEDEILPI 394

Query: 65  LQFSYDSIEDE-AKLILFLCCVFPDDHRINLKDLQMYALSMRLLNGISAWRDARNRVIKL 124
           L++SYD+++ E  KL    C +FP+DH I   DL  Y +    ++       A N+  ++
Sbjct: 395 LKYSYDNLKSEQLKLCFQYCALFPEDHNIEKNDLVDYWIGEGFIDRNKG--KAENQGYEI 454

Query: 125 VDDLKSSSLLLKSDNGNDCVQMHDVVHDVAKYIASKIHKMPS--------LSYGSGESE- 184
           +  L  S LL+  +   + V+MHDVV ++A +IAS   K            S    E E 
Sbjct: 455 IGILVRSCLLM--EENQETVKMHDVVREMALWIASDFGKQKENFIVQAGLQSRNIPEIEK 514

Query: 185 WRDEDICGSYNAIYINCKKFLNFPPKLEFPNLQLFILSGVSDFRSAIRIPDTFFEGMKEL 244
           W+   +    + ++ N +   + P   E P L   +L    +F     I  +FF  M  L
Sbjct: 515 WK---VARRVSLMFNNIESIRDAP---ESPQLITLLLR--KNFLG--HISSSFFRLMPML 574

Query: 245 RVLDV---RTMCFAPSSWTSLTQSLKNLQTLCLTMREGSDF-GTIGELKKLEILKIISSS 304
            VLD+   R +   P+  +       +LQ L L+      +   + EL+KL  L +  + 
Sbjct: 575 VVLDLSMNRDLRHLPNEISECV----SLQYLSLSRTRIRIWPAGLVELRKLLYLNLEYTR 634

Query: 305 FFQVLPASMSELTQLKVLHILCPGLKLIHPNILTSMRRLEEL-----IVGCDYKRWGDEV 364
             + +   +S LT LKVL +   G     P +L  ++ LE L      +G          
Sbjct: 635 MVESI-CGISGLTSLKVLRLFVSGFP-EDPCVLNELQLLENLQTLTITLGL--------A 694

Query: 365 TYKNKLIKNVKLSELDCLTRLLFLKLNIQNVDILSNELSLDRAQSLEEFHIQVGNYVYSL 424
           +   + + N +L+   C   L    LN Q+  ++S   ++D   SL+E H    + ++ +
Sbjct: 695 SILEQFLSNQRLA--SCTRALRIENLNPQS-SVISFVATMD---SLQELHF-ADSDIWEI 754

Query: 425 RSDKFKTSLELETKSPIGSIGGVLQIILQKCENLVVKDSVDFTKLFCNPNGNYGYPRLKH 484
           +  + +T L L   +       + Q+ L+ C  L      D T L   PN       L  
Sbjct: 755 KVKRNETVLPLHIPTTTTFFPNLSQVSLEFCTRL-----RDLTWLIFAPN-------LTV 814

Query: 485 LQII----IREQDEDMPHGIRNITPHFDLQ---------LWHHNNGLTANSFCKLQRICI 544
           L++I    ++E         +N+ P  +L+         L H + G     F  LQ+I +
Sbjct: 815 LRVISASDLKEVINKEKAEQQNLIPFQELKELRLENVQMLKHIHRG--PLPFPCLQKILV 874

Query: 545 NGCNKL-------TSVFPSHMVISLKFLNTLEVRYCKLVERIFEVEEQSNGVNQVMPMVS 583
           NGC++L       TSV    +VI           + K +E I E E++           +
Sbjct: 875 NGCSELRKLPLNFTSVPRGDLVIE---------AHKKWIE-ILEWEDE-----------A 881

BLAST of Lsi10G006250 vs. TAIR 10
Match: AT1G63350.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 90.5 bits (223), Expect = 7.6e-18
Identity = 84/336 (25.00%), Postives = 157/336 (46.73%), Query Frame = 0

Query: 5   IQEIAKDVVKECGGLPIALKILAKALKGKR-VEIWKDTCKKLKDPILVNIPGVSDQLYSC 64
           I+E+++ V K+C GLP+AL ++++ +  KR V+ W+     L +       G+ D++   
Sbjct: 333 IRELSRVVAKKCCGLPLALNVVSETMSCKRTVQEWRHAIYVL-NSYAAKFSGMDDKILPL 392

Query: 65  LQFSYDSIE-DEAKLILFLCCVFPDDHRINLKDLQMYALSMRLLNGISAWRDARNRVIKL 124
           L++SYDS++ ++ K+ L  C +FP+D +I  ++L  Y +   +++G      A N+  ++
Sbjct: 393 LKYSYDSLKGEDVKMCLLYCALFPEDAKIRKENLIEYWICEEIIDGSEGIDKAENQGYEI 452

Query: 125 VDDLKSSSLLLKSD--NGNDCVQMHDVVHDVAKYIASKIHKMPSLSYGSGESEWRDEDIC 184
           +  L  +SLL++    +G + V +HDVV ++A +IAS + K             R+    
Sbjct: 453 IGSLVRASLLMEEVELDGANIVCLHDVVREMALWIASDLGKQNEAFIVRASVGLREILKV 512

Query: 185 GSYNAI---YINCKKFLNFPPKLEFPNLQLFILSGVSDFRSAIRIPDTFFEGMKELRVLD 244
            ++N +    +      +   +L+   L   +L          +I   FF  M +L VLD
Sbjct: 513 ENWNVVRRMSLMKNNIAHLDGRLDCMELTTLLLQST----HLEKISSEFFNSMPKLAVLD 572

Query: 245 VR----------------TMCFAPSSWTSLTQSLKNLQTL----CLTMREGSDFGT---I 304
           +                 ++ +   S T +    K LQ L     L +   S  G+   I
Sbjct: 573 LSGNYYLSELPNGISELVSLQYLNLSSTGIRHLPKGLQELKKLIHLYLERTSQLGSMVGI 632

Query: 305 GELKKLEILKIISSSFFQVLPASMSELTQLKVLHIL 311
             L  L++LK+  SS+   L  ++ EL  L+ L +L
Sbjct: 633 SCLHNLKVLKLSGSSYAWDLD-TVKELEALEHLEVL 662

BLAST of Lsi10G006250 vs. TAIR 10
Match: AT5G43730.1 (Disease resistance protein (CC-NBS-LRR class) family )

HSP 1 Score: 82.4 bits (202), Expect = 2.1e-15
Identity = 93/345 (26.96%), Postives = 157/345 (45.51%), Query Frame = 0

Query: 5   IQEIAKDVVKECGGLPIALKILAKALKGKR-VEIWKDTCKKLKDPILVNIPGVSDQLYSC 64
           I  +A+ V  +C GLP+AL ++ KA+  K  V+ W+     L  P     PG+ +++   
Sbjct: 332 IPALARIVAAKCHGLPLALNVIGKAMVCKETVQEWRHAINVLNSP-GHKFPGMEERILPI 391

Query: 65  LQFSYDSIED-EAKLILFLCCVFPDDHRINLKDLQMYALSMRLLNGISAWRDARNRVIKL 124
           L+FSYDS+++ E KL    C +FP+D  I    L  Y +    +N         N+   +
Sbjct: 392 LKFSYDSLKNGEIKLCFLYCSLFPEDFEIEKDKLIEYWICEGYINPNRYEDGGTNQGYDI 451

Query: 125 VDDLKSSSLLLKSDNGNDCVQMHDVVHDVAKYIASKI-HKMPSLSYGSG--------ESE 184
           +  L  + LL++ +   D V+MHDV+ ++A +I S   ++  ++   SG        +  
Sbjct: 452 IGLLVRAHLLIECEL-TDKVKMHDVIREMALWINSDFGNQQETICVKSGAHVRLIPNDIS 511

Query: 185 WRDEDICGSYNAIYINCKKFLNFPPKLEFPNLQLFILSGVSDFRSAIRIPDTFFEGMKEL 244
           W   +I    + I    +K    P     PNL   +L     +   + I   FF  M +L
Sbjct: 512 W---EIVRQMSLISTQVEKIACSP---NCPNLSTLLL----PYNKLVDISVGFFLFMPKL 571

Query: 245 RVLDVRTMCFAPSSWTSL-----TQSLKNLQTLCLTMREGSDFGTIGELKKLEILKIISS 304
            VLD+ T      +W+ +       +L +LQ L L++  G     +G LKKL  L  ++ 
Sbjct: 572 VVLDLST------NWSLIELPEEISNLGSLQYLNLSL-TGIKSLPVG-LKKLRKLIYLNL 631

Query: 305 SFFQVLPASMSELTQLKVLHI--LCPGLKLIHPNILTSMRRLEEL 332
            F  VL + +   T L  L +  L   L  +   I+  ++RL+ L
Sbjct: 632 EFTNVLESLVGIATTLPNLQVLKLFYSLFCVDDIIMEELQRLKHL 656