Lsi10G006240 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G006240
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPyridoxal phosphate-dependent transferases superfamily protein
Locationchr10: 8727252 .. 8730095 (-)
RNA-Seq ExpressionLsi10G006240
SyntenyLsi10G006240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATCATTCACTATGGAAGCCTCTATCTCACTGTGCTGCTTTGATTATGGATAAAAAGAGTAGAAAAAAAGATGGGTCCGATTCAGCGATGGAGATGGATATCAAGAAACACAAACTGATTCTTCGAAAACTTGAAGAACACAAACTTAGAGAAGCTCTTGAAGAAGCTTCTGAAGATGGGTCTCTCTTCAAGTCGCAAGATGTGGGCTCTGAGCCATTGCCTAACGATGACAACAATGGCTTGGGCCGATCTCGGTCGCTTGCTAGACTTCAAGCTCAGCGGGAGTTCCTCAAGGCCACAGCTATGGCAGCCGATCGTACATATGAATCTGATGATGCCATTCCTGATCTTCATGAAGCTTTCTCTAAGTTTCTCACAATGTACCCAAAGTATCAGTCCTCAGAGAAGATTGATCAGCTTCGTTCGAATGAATATTCTCATTTGATTAAGGTATGTCTTGATTACTGTGGATTTGGGCTGTTTTCTTATGTTCAGAGTCTTCATTATTGGGAGTCTTCTACATTTAGTTTGTCTGAGATTGCTGCAAATTTGAGTAATCAAGCTCTTTATGGTGGTGCTGAGAGAGGGACTGTAGAACATGATATAAAGAGTAGGATTATGGATCATTTGAACATACCTGAGCATGAATATGGCCTTGTTTTTACTGTTAGTAGAGGGTCTGCTTTTAAACTGCTGGCTGAATCATACCCTTTCAATTCCAACAAGAAATTGTTGACTATGTTTGATTATGAGAGCCAATCTGTGAATTGGATGGCTCAGTGTGCTAGGGAGAAGGGTGCTAAGGCATATAGTGCTTGGTTTAAATGGCCAACTTTGAAACTTTGCTCAACCGATTTGCGGAAACAGATAACGAACAAGAGGAGGAAGAAGAAGGATTCTGTTGGTCTGTTTGTGTTTCCTGTTCAGTCTAGAGTGACTGGTGCTAAGTATTCATACCAGTGGATGGCTTTGGCACAACAGAACAATTGGCATGTTTTGCTTGATGCTGGGTCGTTAGGTCCAAAGGACATGGATTCACTTGGTTTATCGCTTTTTCGACCAGATTTTATCATAACGTCGTTTTATAGGGTTTTCGGGTATGATCCTACCGGTTTTGGTTGTCTTCTGATAAAGAGATCAGTGATGGGAAGTTTACAAACTCGGTCTGGATGTACTGGCTCTGGAATGGTGAAGATAACCCCTGAGTATCCCATGTACCTGAGTGACTCGGTGGATGATCTTGACAGAGTGGGTCGTTTTGAAGACGACCAAGTTGCTGGGGTTGAGGATAAAACATCTGAAACTCGTCAGGGTTTGCAACTGCCTGCTTTCTCTGGTGCCTTCACGTCTGCTCAGGTGAGGGATGTTTATGAAACAGAGATGGATCAAGATAACAGCTCTGACAGAGATGGAACAAGCACCATACTTGAGGAAAGTGAGACTATTTCTCTTGGGGATGTGATGAAGAGCCCGATATTCAGTGAAGATGAATCGTCGGATTGTTCGATTTGGATTGACTTAGGACAGAGTCCACTAGGATCTGATAATGCAGGTCAATCGCACAAAGTGGAAATTGCTTCTCCCCTGCCTCAGCATTGGTTAAAAGGAAAGAAGAACAAACTACTCTCACCTAAGCCAACTTCTAAAATCCATAGTGAACCAACATACGACAATGACAAAGATTTTAACTTGGGGCCTTATGATGAGCAACCTGTAATATCTTTTGATGCTGCTGTTCAGTCAGTCTGCCAGGAACTGGACTGCGTCGAGGAGGTTCCCAGAGAATTATTTGCAGAAACAAGTGACACGTCGGCTAATAGCAAAAACGACTTCAATAACAGAGTTGTTACAGAGATCCACGAAGTGACAGAAGCTAGTAAACCACTTTCCAACGGTTCCTCAATGAATTCTACGTTAAACAATGGATTTCATGTTGACATTTCTGCCACCGATTTTCGTTATCGTGGACTAGAAAATGGTACAACATCAGAAATGTGTCCAGAGATGAAGGAGAGTGCCATTAGAAGGGAAACAGAAGGTGAATTCAGGTTGTTGGGGAGACGGGAAGGGAACAAACATGTTGGTGGAAGGTTTTTTGGTTTGGAAGAGAGTAATATCCAAAGCCGAGGAAGACGAGTTTCTTTTAGGATGGAAGAGAATGGTAAGGAGCACTTAAGCCATAACATCGAGCCTGGAGAAGTATCAGTGACGAGCTTGGATGATGAAGATTACACCAGCAATGGAGAATATGATGATGAGGAAGAGTGGAACAGGAGGGAGCCTGAGATTATATGTCGACATCTCGATCACATAAATATGTTAGGTCTGAACAAGACAACACTTCGACTTCGATTTTTGATCAATTGGCTCGTCACGTCATTGCTTCAACTAAAGTTTCCCGGTTCAGAAGGAAGCAACAAAGTGAACCTAGTACAAATTTATGGACCAAAAATAAAATATGAAAGGGGAGCAGCAGTAGCTTTCAATGTGAGAAACAGAAACAGGGGACTAATCAATCCAGAGTTTGTTCAGAAGCTGGCTGAAAGAGATGGCATATCTCTTGGCATTGGATTCCTCAGTCACATTTGGGTTTTGGACAGCTCGAAACGGCAACATGGTGTTTTAAATCTTGAAGAATCATCCCTGTGCAGGCAAACAAAAAATGGCAGGCGTGGGAAGCACGGATTTGCACGGCTTGAAGTCGTAACAGCTTCGTTGGGATTCTTGACGAACTTCGAAGACGTTTATAAACTGTGGGGATTTGTGGCCAAGTTCTTAAATCCTTCCTTTATCAGAGAGGGAACACTTGCTACTGTTGAGGAAGGTTCTGAAACAACCTGA

mRNA sequence

ATGCATCATTCACTATGGAAGCCTCTATCTCACTGTGCTGCTTTGATTATGGATAAAAAGAGTAGAAAAAAAGATGGGTCCGATTCAGCGATGGAGATGGATATCAAGAAACACAAACTGATTCTTCGAAAACTTGAAGAACACAAACTTAGAGAAGCTCTTGAAGAAGCTTCTGAAGATGGGTCTCTCTTCAAGTCGCAAGATGTGGGCTCTGAGCCATTGCCTAACGATGACAACAATGGCTTGGGCCGATCTCGGTCGCTTGCTAGACTTCAAGCTCAGCGGGAGTTCCTCAAGGCCACAGCTATGGCAGCCGATCGTACATATGAATCTGATGATGCCATTCCTGATCTTCATGAAGCTTTCTCTAAGTTTCTCACAATGTACCCAAAGTATCAGTCCTCAGAGAAGATTGATCAGCTTCGTTCGAATGAATATTCTCATTTGATTAAGGTATGTCTTGATTACTGTGGATTTGGGCTGTTTTCTTATGTTCAGAGTCTTCATTATTGGGAGTCTTCTACATTTAGTTTGTCTGAGATTGCTGCAAATTTGAGTAATCAAGCTCTTTATGGTGGTGCTGAGAGAGGGACTGTAGAACATGATATAAAGAGTAGGATTATGGATCATTTGAACATACCTGAGCATGAATATGGCCTTGTTTTTACTGTTAGTAGAGGGTCTGCTTTTAAACTGCTGGCTGAATCATACCCTTTCAATTCCAACAAGAAATTGTTGACTATGTTTGATTATGAGAGCCAATCTGTGAATTGGATGGCTCAGTGTGCTAGGGAGAAGGGTGCTAAGGCATATAGTGCTTGGTTTAAATGGCCAACTTTGAAACTTTGCTCAACCGATTTGCGGAAACAGATAACGAACAAGAGGAGGAAGAAGAAGGATTCTGTTGGTCTGTTTGTGTTTCCTGTTCAGTCTAGAGTGACTGGTGCTAAGTATTCATACCAGTGGATGGCTTTGGCACAACAGAACAATTGGCATGTTTTGCTTGATGCTGGGTCGTTAGGTCCAAAGGACATGGATTCACTTGGTTTATCGCTTTTTCGACCAGATTTTATCATAACGTCGTTTTATAGGGTTTTCGGGTATGATCCTACCGGTTTTGGTTGTCTTCTGATAAAGAGATCAGTGATGGGAAGTTTACAAACTCGGTCTGGATGTACTGGCTCTGGAATGGTGAAGATAACCCCTGAGTATCCCATGTACCTGAGTGACTCGGTGGATGATCTTGACAGAGTGGGTCGTTTTGAAGACGACCAAGTTGCTGGGGTTGAGGATAAAACATCTGAAACTCGTCAGGGTTTGCAACTGCCTGCTTTCTCTGGTGCCTTCACGTCTGCTCAGGTGAGGGATGTTTATGAAACAGAGATGGATCAAGATAACAGCTCTGACAGAGATGGAACAAGCACCATACTTGAGGAAAGTGAGACTATTTCTCTTGGGGATGTGATGAAGAGCCCGATATTCAGTGAAGATGAATCGTCGGATTGTTCGATTTGGATTGACTTAGGACAGAGTCCACTAGGATCTGATAATGCAGGTCAATCGCACAAAGTGGAAATTGCTTCTCCCCTGCCTCAGCATTGGTTAAAAGGAAAGAAGAACAAACTACTCTCACCTAAGCCAACTTCTAAAATCCATAGTGAACCAACATACGACAATGACAAAGATTTTAACTTGGGGCCTTATGATGAGCAACCTGTAATATCTTTTGATGCTGCTGTTCAGTCAGTCTGCCAGGAACTGGACTGCGTCGAGGAGGTTCCCAGAGAATTATTTGCAGAAACAAGTGACACGTCGGCTAATAGCAAAAACGACTTCAATAACAGAGTTGTTACAGAGATCCACGAAGTGACAGAAGCTAGTAAACCACTTTCCAACGGTTCCTCAATGAATTCTACGTTAAACAATGGATTTCATGTTGACATTTCTGCCACCGATTTTCGTTATCGTGGACTAGAAAATGGTACAACATCAGAAATGTGTCCAGAGATGAAGGAGAGTGCCATTAGAAGGGAAACAGAAGGTGAATTCAGGTTGTTGGGGAGACGGGAAGGGAACAAACATGTTGGTGGAAGGTTTTTTGGTTTGGAAGAGAGTAATATCCAAAGCCGAGGAAGACGAGTTTCTTTTAGGATGGAAGAGAATGGTAAGGAGCACTTAAGCCATAACATCGAGCCTGGAGAAGTATCAGTGACGAGCTTGGATGATGAAGATTACACCAGCAATGGAGAATATGATGATGAGGAAGAGTGGAACAGGAGGGAGCCTGAGATTATATGTCGACATCTCGATCACATAAATATGTTAGGTCTGAACAAGACAACACTTCGACTTCGATTTTTGATCAATTGGCTCGTCACGTCATTGCTTCAACTAAAGTTTCCCGGTTCAGAAGGAAGCAACAAAGTGAACCTAGTACAAATTTATGGACCAAAAATAAAATATGAAAGGGGAGCAGCAGTAGCTTTCAATGTGAGAAACAGAAACAGGGGACTAATCAATCCAGAGTTTGTTCAGAAGCTGGCTGAAAGAGATGGCATATCTCTTGGCATTGGATTCCTCAGTCACATTTGGGTTTTGGACAGCTCGAAACGGCAACATGGTGTTTTAAATCTTGAAGAATCATCCCTGTGCAGGCAAACAAAAAATGGCAGGCGTGGGAAGCACGGATTTGCACGGCTTGAAGTCGTAACAGCTTCGTTGGGATTCTTGACGAACTTCGAAGACGTTTATAAACTGTGGGGATTTGTGGCCAAGTTCTTAAATCCTTCCTTTATCAGAGAGGGAACACTTGCTACTGTTGAGGAAGGTTCTGAAACAACCTGA

Coding sequence (CDS)

ATGCATCATTCACTATGGAAGCCTCTATCTCACTGTGCTGCTTTGATTATGGATAAAAAGAGTAGAAAAAAAGATGGGTCCGATTCAGCGATGGAGATGGATATCAAGAAACACAAACTGATTCTTCGAAAACTTGAAGAACACAAACTTAGAGAAGCTCTTGAAGAAGCTTCTGAAGATGGGTCTCTCTTCAAGTCGCAAGATGTGGGCTCTGAGCCATTGCCTAACGATGACAACAATGGCTTGGGCCGATCTCGGTCGCTTGCTAGACTTCAAGCTCAGCGGGAGTTCCTCAAGGCCACAGCTATGGCAGCCGATCGTACATATGAATCTGATGATGCCATTCCTGATCTTCATGAAGCTTTCTCTAAGTTTCTCACAATGTACCCAAAGTATCAGTCCTCAGAGAAGATTGATCAGCTTCGTTCGAATGAATATTCTCATTTGATTAAGGTATGTCTTGATTACTGTGGATTTGGGCTGTTTTCTTATGTTCAGAGTCTTCATTATTGGGAGTCTTCTACATTTAGTTTGTCTGAGATTGCTGCAAATTTGAGTAATCAAGCTCTTTATGGTGGTGCTGAGAGAGGGACTGTAGAACATGATATAAAGAGTAGGATTATGGATCATTTGAACATACCTGAGCATGAATATGGCCTTGTTTTTACTGTTAGTAGAGGGTCTGCTTTTAAACTGCTGGCTGAATCATACCCTTTCAATTCCAACAAGAAATTGTTGACTATGTTTGATTATGAGAGCCAATCTGTGAATTGGATGGCTCAGTGTGCTAGGGAGAAGGGTGCTAAGGCATATAGTGCTTGGTTTAAATGGCCAACTTTGAAACTTTGCTCAACCGATTTGCGGAAACAGATAACGAACAAGAGGAGGAAGAAGAAGGATTCTGTTGGTCTGTTTGTGTTTCCTGTTCAGTCTAGAGTGACTGGTGCTAAGTATTCATACCAGTGGATGGCTTTGGCACAACAGAACAATTGGCATGTTTTGCTTGATGCTGGGTCGTTAGGTCCAAAGGACATGGATTCACTTGGTTTATCGCTTTTTCGACCAGATTTTATCATAACGTCGTTTTATAGGGTTTTCGGGTATGATCCTACCGGTTTTGGTTGTCTTCTGATAAAGAGATCAGTGATGGGAAGTTTACAAACTCGGTCTGGATGTACTGGCTCTGGAATGGTGAAGATAACCCCTGAGTATCCCATGTACCTGAGTGACTCGGTGGATGATCTTGACAGAGTGGGTCGTTTTGAAGACGACCAAGTTGCTGGGGTTGAGGATAAAACATCTGAAACTCGTCAGGGTTTGCAACTGCCTGCTTTCTCTGGTGCCTTCACGTCTGCTCAGGTGAGGGATGTTTATGAAACAGAGATGGATCAAGATAACAGCTCTGACAGAGATGGAACAAGCACCATACTTGAGGAAAGTGAGACTATTTCTCTTGGGGATGTGATGAAGAGCCCGATATTCAGTGAAGATGAATCGTCGGATTGTTCGATTTGGATTGACTTAGGACAGAGTCCACTAGGATCTGATAATGCAGGTCAATCGCACAAAGTGGAAATTGCTTCTCCCCTGCCTCAGCATTGGTTAAAAGGAAAGAAGAACAAACTACTCTCACCTAAGCCAACTTCTAAAATCCATAGTGAACCAACATACGACAATGACAAAGATTTTAACTTGGGGCCTTATGATGAGCAACCTGTAATATCTTTTGATGCTGCTGTTCAGTCAGTCTGCCAGGAACTGGACTGCGTCGAGGAGGTTCCCAGAGAATTATTTGCAGAAACAAGTGACACGTCGGCTAATAGCAAAAACGACTTCAATAACAGAGTTGTTACAGAGATCCACGAAGTGACAGAAGCTAGTAAACCACTTTCCAACGGTTCCTCAATGAATTCTACGTTAAACAATGGATTTCATGTTGACATTTCTGCCACCGATTTTCGTTATCGTGGACTAGAAAATGGTACAACATCAGAAATGTGTCCAGAGATGAAGGAGAGTGCCATTAGAAGGGAAACAGAAGGTGAATTCAGGTTGTTGGGGAGACGGGAAGGGAACAAACATGTTGGTGGAAGGTTTTTTGGTTTGGAAGAGAGTAATATCCAAAGCCGAGGAAGACGAGTTTCTTTTAGGATGGAAGAGAATGGTAAGGAGCACTTAAGCCATAACATCGAGCCTGGAGAAGTATCAGTGACGAGCTTGGATGATGAAGATTACACCAGCAATGGAGAATATGATGATGAGGAAGAGTGGAACAGGAGGGAGCCTGAGATTATATGTCGACATCTCGATCACATAAATATGTTAGGTCTGAACAAGACAACACTTCGACTTCGATTTTTGATCAATTGGCTCGTCACGTCATTGCTTCAACTAAAGTTTCCCGGTTCAGAAGGAAGCAACAAAGTGAACCTAGTACAAATTTATGGACCAAAAATAAAATATGAAAGGGGAGCAGCAGTAGCTTTCAATGTGAGAAACAGAAACAGGGGACTAATCAATCCAGAGTTTGTTCAGAAGCTGGCTGAAAGAGATGGCATATCTCTTGGCATTGGATTCCTCAGTCACATTTGGGTTTTGGACAGCTCGAAACGGCAACATGGTGTTTTAAATCTTGAAGAATCATCCCTGTGCAGGCAAACAAAAAATGGCAGGCGTGGGAAGCACGGATTTGCACGGCTTGAAGTCGTAACAGCTTCGTTGGGATTCTTGACGAACTTCGAAGACGTTTATAAACTGTGGGGATTTGTGGCCAAGTTCTTAAATCCTTCCTTTATCAGAGAGGGAACACTTGCTACTGTTGAGGAAGGTTCTGAAACAACCTGA

Protein sequence

MHHSLWKPLSHCAALIMDKKSRKKDGSDSAMEMDIKKHKLILRKLEEHKLREALEEASEDGSLFKSQDVGSEPLPNDDNNGLGRSRSLARLQAQREFLKATAMAADRTYESDDAIPDLHEAFSKFLTMYPKYQSSEKIDQLRSNEYSHLIKVCLDYCGFGLFSYVQSLHYWESSTFSLSEIAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFNSNKKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKDSVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIITSFYRVFGYDPTGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSVDDLDRVGRFEDDQVAGVEDKTSETRQGLQLPAFSGAFTSAQVRDVYETEMDQDNSSDRDGTSTILEESETISLGDVMKSPIFSEDESSDCSIWIDLGQSPLGSDNAGQSHKVEIASPLPQHWLKGKKNKLLSPKPTSKIHSEPTYDNDKDFNLGPYDEQPVISFDAAVQSVCQELDCVEEVPRELFAETSDTSANSKNDFNNRVVTEIHEVTEASKPLSNGSSMNSTLNNGFHVDISATDFRYRGLENGTTSEMCPEMKESAIRRETEGEFRLLGRREGNKHVGGRFFGLEESNIQSRGRRVSFRMEENGKEHLSHNIEPGEVSVTSLDDEDYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNKTTLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLINPEFVQKLAERDGISLGIGFLSHIWVLDSSKRQHGVLNLEESSLCRQTKNGRRGKHGFARLEVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLATVEEGSETT
Homology
BLAST of Lsi10G006240 vs. ExPASy Swiss-Prot
Match: Q16P90 (Molybdenum cofactor sulfurase 3 OS=Aedes aegypti OX=7159 GN=mal3 PE=3 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 3.2e-16
Identity = 79/269 (29.37%), Postives = 113/269 (42.01%), Query Frame = 0

Query: 145 EYSHLIKVC-LDYCGFGLFSYVQSLHYWESSTFSLSEIAANLSNQALYGGAERGTVEHD- 204
           E+S L + C LD+ G  L        Y +S   S+ E  A    Q LY          D 
Sbjct: 22  EFSRLKEKCYLDHAGTTL--------YADSQIRSVCEGLA----QNLYCNPHTSRTTEDL 81

Query: 205 ---IKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFNSNKKLLTMFDYESQSVNWM 264
              ++ R++ H N    EY L+FT    ++ KLLAESY F      + + D  +  +   
Sbjct: 82  LDQVRYRVLRHFNTRSSEYSLIFTSGTTASLKLLAESYEFAPEGAFVYLKDSHTSVLGMR 141

Query: 265 AQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKDSVGLFVFPVQSRVTGAKYS 324
                E+          +P  +     L K++ +  R   +   L VFP Q    G KY 
Sbjct: 142 EIVGTER---------IYPVER---EQLLKELDSSERSDSEHSSLIVFPAQCNFNGVKYP 201

Query: 325 YQWMALAQQN--------NWHVLLDAGSLGPKDMDSLGLSLFRPDFIITSFYRVFGYDPT 384
            + +   Q+N         + V LDA S        L LS ++PDF+  SFY++FGY PT
Sbjct: 202 LELVRKIQRNGISGYGKERFRVCLDAASF--VSTSFLDLSKYQPDFVCLSFYKIFGY-PT 261

Query: 385 GFGCLLIKRSVMGSLQTRSGCTGSGMVKI 401
           G G LL+  +     Q R    G G VKI
Sbjct: 262 GLGALLVHHTAAD--QLRKKYYGGGTVKI 261

BLAST of Lsi10G006240 vs. ExPASy Swiss-Prot
Match: Q16GH0 (Molybdenum cofactor sulfurase 1 OS=Aedes aegypti OX=7159 GN=mal1 PE=3 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 4.2e-16
Identity = 85/297 (28.62%), Postives = 124/297 (41.75%), Query Frame = 0

Query: 144 NEYSHLIKVC-LDYCGFGLFSYVQSLHYWESSTFSLSEIAANLSNQALYGGAERGTVEHD 203
           NE+S L + C LD+ G  L        Y +S   S+ E  A    Q LY          D
Sbjct: 21  NEFSRLKEKCYLDHAGTTL--------YADSQIRSVCEGLA----QNLYCNPHTSRTTED 80

Query: 204 ----IKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFNSNKKLLTMFDYESQSVNW 263
               ++ R++ H N    EY L+FT    ++ KLLAES+ F      + + D  +  +  
Sbjct: 81  LLDQVRYRVLRHFNTRSSEYSLIFTSGTTASLKLLAESFEFAPEGAFVYLKDSHTSVLGM 140

Query: 264 MAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKDSVGLFVFPVQSRVTGAKY 323
                 E+          +P  +     L K++ +  R   +   L VFP Q    G KY
Sbjct: 141 REIVGTER---------IYPVER---EQLLKELDSSERSDNEHSSLIVFPAQCNFNGVKY 200

Query: 324 SYQWMALAQQN--------NWHVLLDAGSLGPKDMDSLGLSLFRPDFIITSFYRVFGYDP 383
             + +   Q++         + V LDA S        L LS ++PDF+  SFY++FGY P
Sbjct: 201 PLELVRKIQRDGISGYGKERFRVCLDAASF--VSTSFLDLSKYQPDFVCLSFYKIFGY-P 260

Query: 384 TGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSVDDLDRVGRFEDDQVA 428
           TG G LL+  +     Q R    G G VKI     ++    V     V RFED  +A
Sbjct: 261 TGLGALLVHHTAAD--QLRKKYYGGGTVKIAMAGRIF---HVKRDPLVERFEDGTLA 285

BLAST of Lsi10G006240 vs. ExPASy Swiss-Prot
Match: Q9C5X8 (Molybdenum cofactor sulfurase OS=Arabidopsis thaliana OX=3702 GN=ABA3 PE=1 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 7.1e-16
Identity = 76/295 (25.76%), Postives = 138/295 (46.78%), Query Frame = 0

Query: 120 EAFSKFLTMYPKYQSSEK-IDQLRSNEYSHLIK--VCLDYCGFGLFSYVQSLHYWESSTF 179
           EAF K    Y  Y    K I ++R  E+  L K  V LD+ G  L+S +Q  + ++  T 
Sbjct: 2   EAFLKEFGDYYGYPDGPKNIQEIRDTEFKRLDKGVVYLDHAGSTLYSELQMEYIFKDFT- 61

Query: 180 SLSEIAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAES 239
             S +  N  +Q+    A    +  D + +++++ N    +Y  +FT    +A KL+ E+
Sbjct: 62  --SNVFGNPHSQSDISSATSDLIA-DARHQVLEYFNASPEDYSCLFTSGATAALKLVGET 121

Query: 240 YPFNSNKKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFK------------WPTLKLCS 299
           +P+  +   L   +    SV  + + A  +GA A +   +             P++K+  
Sbjct: 122 FPWTQDSNFLYTME-NHNSVLGIREYALAQGASACAVDIEEAANQPGQLTNSGPSIKVKH 181

Query: 300 TDLRKQITNKRRKKK---DSVGLFVFPVQSRVTGAKYSYQWMALAQQN------------ 359
             ++ + T+K +K++   ++  LF FP +   +G +++   + L ++N            
Sbjct: 182 RAVQMRNTSKLQKEESRGNAYNLFAFPSECNFSGLRFNLDLVKLMKENTETVLQGSPFSK 241

Query: 360 --NWHVLLDAG---SLGPKDMDSLGLSLFRPDFIITSFYRVFGYDPTGFGCLLIK 380
              W VL+DA    +  P D     LS +  DF++ SFY++FGY PTG G LL++
Sbjct: 242 SKRWMVLIDAAKGCATLPPD-----LSEYPADFVVLSFYKLFGY-PTGLGALLVR 285

BLAST of Lsi10G006240 vs. ExPASy Swiss-Prot
Match: Q8LGM7 (Molybdenum cofactor sulfurase OS=Solanum lycopersicum OX=4081 GN=FLACCA PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 2.7e-15
Identity = 74/294 (25.17%), Postives = 134/294 (45.58%), Query Frame = 0

Query: 120 EAFSKFLTMYPKYQSSEK-IDQLRSNEYSHL-IKVCLDYCGFGLFSYVQSLHYWESSTFS 179
           E F K    Y  Y +S K ID++R+ E+  L   V LD+ G  L+S  Q    ++    +
Sbjct: 8   EQFLKEFGSYYGYANSPKNIDEIRATEFKRLNDTVYLDHAGATLYSESQMEAVFKDLNST 67

Query: 180 L-----SEIAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKL 239
           L     S+   +L+ + + G A         + +++   N    EY  +FT    +A KL
Sbjct: 68  LYGNPHSQSTCSLATEDIVGKA---------RQQVLSFFNASPREYSCIFTSGATAALKL 127

Query: 240 LAESYPFNSNKKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFK----------WPTLKL 299
           + E++P++SN   +   +    SV  + + A  KGA A++   +             LKL
Sbjct: 128 VGETFPWSSNSSFMYSME-NHNSVLGIREYALSKGAAAFAVDIEDTHVGESESPQSNLKL 187

Query: 300 CSTDLRKQITN---KRRKKKDSVGLFVFPVQSRVTGAKYSYQWMALAQQNN--------- 359
               ++++      K     ++  LF FP +   +G K+    + + ++ +         
Sbjct: 188 TQHHIQRRNEGGVLKEGMTGNTYNLFAFPSECNFSGRKFDPNLIKIIKEGSERILESSQY 247

Query: 360 ----WHVLLDAGSLGPKDMDSLGLSLFRPDFIITSFYRVFGYDPTGFGCLLIKR 381
               W VL+DA      +  +  LS+F+ DF++ SFY++FGY PTG G L++++
Sbjct: 248 SRGCWLVLIDAAKGCATNPPN--LSMFKADFVVFSFYKLFGY-PTGLGALIVRK 288

BLAST of Lsi10G006240 vs. ExPASy Swiss-Prot
Match: Q7QFL7 (Molybdenum cofactor sulfurase OS=Anopheles gambiae OX=7165 GN=mal PE=3 SV=5)

HSP 1 Score: 82.4 bits (202), Expect = 3.0e-14
Identity = 87/306 (28.43%), Postives = 123/306 (40.20%), Query Frame = 0

Query: 137 KIDQLRSNEYSHLIKVC-LDYCGFGLFSYVQSLHYWESSTFSLSEIAANLSNQALYGGAE 196
           KI+Q    ++S L   C LD+ G  L        Y ES   ++ E+ A      LY    
Sbjct: 14  KIEQ----DFSRLADKCYLDHAGTAL--------YGESQLRAVQELLAG----GLYCNPH 73

Query: 197 RGTVEHD----IKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFNSNKKLLTMFDY 256
                 D    ++ R++        +Y LVFT    ++ KL+AES+ F         F Y
Sbjct: 74  TSRTMEDLIDLVRYRVLRWFQTRPADYSLVFTSGTTASLKLVAESFEFGPGDAEPGSFVY 133

Query: 257 ---ESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKDSVGLFVFP 316
                 SV  M +  R    +        P  +        +  + RR+      L VFP
Sbjct: 134 LRDSHTSVLGMRELVRTGRVQ--------PIERAELLQALNEPEDPRRQHPHRPSLLVFP 193

Query: 317 VQSRVTGAKYSYQWMALAQQNN--------WHVLLDAGSLGPKDMDSLGLSLFRPDFIIT 376
            Q    GAKY  +   L ++N         +HV LDA S        L LS +RP F+  
Sbjct: 194 AQCNFNGAKYPLELCELIERNGLRGYGGDAFHVCLDAAS--HVSTSPLDLSRYRPSFVCL 253

Query: 377 SFYRVFGYDPTGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSVDDLDRVGR 427
           SFY++FGY PTG G LL++R     L+ +    G G VKI    P    +  D L    R
Sbjct: 254 SFYKIFGY-PTGLGALLVRRDAEPLLRGKR-YYGGGTVKIALSGPDRFHERRDALP--DR 289

BLAST of Lsi10G006240 vs. ExPASy TrEMBL
Match: A0A5D3CRB4 (Pyridoxal phosphate-dependent transferases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G002560 PE=4 SV=1)

HSP 1 Score: 1777.3 bits (4602), Expect = 0.0e+00
Identity = 888/947 (93.77%), Postives = 919/947 (97.04%), Query Frame = 0

Query: 1   MHHSLWKPLSHCAALIMDKKSRKKDGSDSAMEMDIKKHKLILRKLEEHKLREALEEASED 60
           MHHSLWKPLSHCAALIMDKKSRKKDGSDSA  MDIKKHKLILRKLEEHKLREALEEASED
Sbjct: 1   MHHSLWKPLSHCAALIMDKKSRKKDGSDSA--MDIKKHKLILRKLEEHKLREALEEASED 60

Query: 61  GSLFKSQDVGSEPLPNDDNNGLGRSRSLARLQAQREFLKATAMAADRTYESDDAIPDLHE 120
           GSL KSQDV SEPLPNDDNNGLGRSRSLARLQAQREFLKATAMAADRTYESD  IPDLHE
Sbjct: 61  GSLSKSQDVDSEPLPNDDNNGLGRSRSLARLQAQREFLKATAMAADRTYESDGDIPDLHE 120

Query: 121 AFSKFLTMYPKYQSSEKIDQLRSNEYSHLIKVCLDYCGFGLFSYVQSLHYWESSTFSLSE 180
           AFSKFLTMYPKYQSSEKIDQLRSNEYSHL+KVCLDYCGFGLFSYVQSLHYWESSTFSLSE
Sbjct: 121 AFSKFLTMYPKYQSSEKIDQLRSNEYSHLVKVCLDYCGFGLFSYVQSLHYWESSTFSLSE 180

Query: 181 IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFN 240
           IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFN
Sbjct: 181 IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFN 240

Query: 241 SNKKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKD 300
           +NKKLLTMFDYESQSVNWMAQCAR+KGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKD
Sbjct: 241 TNKKLLTMFDYESQSVNWMAQCARDKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKD 300

Query: 301 SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIIT 360
           SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIIT
Sbjct: 301 SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIIT 360

Query: 361 SFYRVFGYDPTGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSVDDLDRVGR 420
           SFYRVFGYDPTGFGCLLIK+SVMGSLQTRSGCTGSGMVKITPEYPMYLSDS+DDLD VG+
Sbjct: 361 SFYRVFGYDPTGFGCLLIKKSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSMDDLDGVGQ 420

Query: 421 FEDDQVAGVEDKTSETRQGLQLPAFSGAFTSAQVRDVYETEMDQDNSSDRDGTSTILEES 480
           FEDDQVAGV DKTSETRQG QLPAFSGAFTSAQVRD+YETEMD DNSSDRDGTSTILEES
Sbjct: 421 FEDDQVAGVVDKTSETRQGSQLPAFSGAFTSAQVRDIYETEMDHDNSSDRDGTSTILEES 480

Query: 481 ETISLGDVMKSPIFSEDESSDCSIWIDLGQSPLGSDNAGQSHKVEIASPLPQHWLKGKKN 540
           ETISLG+VMKSP+FSEDESSDCSIWIDLGQSPLGSDN GQ +K +IASPLPQHWLKG+KN
Sbjct: 481 ETISLGEVMKSPVFSEDESSDCSIWIDLGQSPLGSDNGGQLYKQKIASPLPQHWLKGRKN 540

Query: 541 KLLSPKPTSKIHSEPTYDNDKDFNLGPYDEQPVISFDAAVQSVCQELDCVEEVPRELFAE 600
           KLLSPKPTSKIHSEPTYDN+K+FN  P DEQPV+SFDAAVQSVCQELDC+EEVP +LFAE
Sbjct: 541 KLLSPKPTSKIHSEPTYDNEKEFNFRPCDEQPVLSFDAAVQSVCQELDCIEEVPGDLFAE 600

Query: 601 TSDTSANSKNDFNNRVVTEIHEVTEASKPLSNGSSMNSTLNNGFHVDISATDFRYRGLEN 660
           TS   AN+K + NNRV TEIHEVTEASKPLSNGSS + T+NNGFH+DIS +DFRYRGLEN
Sbjct: 601 TSTMPANTKINSNNRVDTEIHEVTEASKPLSNGSSKSYTMNNGFHLDISTSDFRYRGLEN 660

Query: 661 GTTSEMCPEMKESAIRRETEGEFRLLGRREGNKHVGGRFFGLEESNIQSRGRRVSFRMEE 720
           GTTSE+CPE+KESAIRRETEGEFRLLGRREG+KHVGGRFFGLEESN+QSRGRRVSFRMEE
Sbjct: 661 GTTSEICPEVKESAIRRETEGEFRLLGRREGSKHVGGRFFGLEESNMQSRGRRVSFRMEE 720

Query: 721 NGKEHLSHNIEPGEVSVTSLDDEDYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNKT 780
           NGKEHLSHNI+PGEVSVTSLDD+DYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNKT
Sbjct: 721 NGKEHLSHNIDPGEVSVTSLDDDDYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNKT 780

Query: 781 TLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLIN 840
           TLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLIN
Sbjct: 781 TLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLIN 840

Query: 841 PEFVQKLAERDGISLGIGFLSHIWVLDSSKRQHGVLNLEESSLCRQTKNGRRGKHGFARL 900
           PEFVQKLAERDGISLGIGFLSHI VLDSSKRQ+GVLNLEESSLCR+TKNGRRGKHGFARL
Sbjct: 841 PEFVQKLAERDGISLGIGFLSHIRVLDSSKRQYGVLNLEESSLCRETKNGRRGKHGFARL 900

Query: 901 EVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLATVEEGSETT 948
           EVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLA VEEGSETT
Sbjct: 901 EVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLAPVEEGSETT 945

BLAST of Lsi10G006240 vs. ExPASy TrEMBL
Match: A0A1S3C752 (uncharacterized protein LOC103497444 OS=Cucumis melo OX=3656 GN=LOC103497444 PE=4 SV=1)

HSP 1 Score: 1777.3 bits (4602), Expect = 0.0e+00
Identity = 888/947 (93.77%), Postives = 919/947 (97.04%), Query Frame = 0

Query: 1   MHHSLWKPLSHCAALIMDKKSRKKDGSDSAMEMDIKKHKLILRKLEEHKLREALEEASED 60
           MHHSLWKPLSHCAALIMDKKSRKKDGSDSA  MDIKKHKLILRKLEEHKLREALEEASED
Sbjct: 1   MHHSLWKPLSHCAALIMDKKSRKKDGSDSA--MDIKKHKLILRKLEEHKLREALEEASED 60

Query: 61  GSLFKSQDVGSEPLPNDDNNGLGRSRSLARLQAQREFLKATAMAADRTYESDDAIPDLHE 120
           GSL KSQDV SEPLPNDDNNGLGRSRSLARLQAQREFLKATAMAADRTYESD  IPDLHE
Sbjct: 61  GSLSKSQDVDSEPLPNDDNNGLGRSRSLARLQAQREFLKATAMAADRTYESDGDIPDLHE 120

Query: 121 AFSKFLTMYPKYQSSEKIDQLRSNEYSHLIKVCLDYCGFGLFSYVQSLHYWESSTFSLSE 180
           AFSKFLTMYPKYQSSEKIDQLRSNEYSHL+KVCLDYCGFGLFSYVQSLHYWESSTFSLSE
Sbjct: 121 AFSKFLTMYPKYQSSEKIDQLRSNEYSHLVKVCLDYCGFGLFSYVQSLHYWESSTFSLSE 180

Query: 181 IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFN 240
           IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFN
Sbjct: 181 IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFN 240

Query: 241 SNKKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKD 300
           +NKKLLTMFDYESQSVNWMAQCAR+KGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKD
Sbjct: 241 TNKKLLTMFDYESQSVNWMAQCARDKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKD 300

Query: 301 SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIIT 360
           SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIIT
Sbjct: 301 SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIIT 360

Query: 361 SFYRVFGYDPTGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSVDDLDRVGR 420
           SFYRVFGYDPTGFGCLLIK+SVMGSLQTRSGCTGSGMVKITPEYPMYLSDS+DDLD VG+
Sbjct: 361 SFYRVFGYDPTGFGCLLIKKSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSMDDLDGVGQ 420

Query: 421 FEDDQVAGVEDKTSETRQGLQLPAFSGAFTSAQVRDVYETEMDQDNSSDRDGTSTILEES 480
           FEDDQVAGV DKTSETRQG QLPAFSGAFTSAQVRD+YETEMD DNSSDRDGTSTILEES
Sbjct: 421 FEDDQVAGVVDKTSETRQGSQLPAFSGAFTSAQVRDIYETEMDHDNSSDRDGTSTILEES 480

Query: 481 ETISLGDVMKSPIFSEDESSDCSIWIDLGQSPLGSDNAGQSHKVEIASPLPQHWLKGKKN 540
           ETISLG+VMKSP+FSEDESSDCSIWIDLGQSPLGSDN GQ +K +IASPLPQHWLKG+KN
Sbjct: 481 ETISLGEVMKSPVFSEDESSDCSIWIDLGQSPLGSDNGGQLYKQKIASPLPQHWLKGRKN 540

Query: 541 KLLSPKPTSKIHSEPTYDNDKDFNLGPYDEQPVISFDAAVQSVCQELDCVEEVPRELFAE 600
           KLLSPKPTSKIHSEPTYDN+K+FN  P DEQPV+SFDAAVQSVCQELDC+EEVP +LFAE
Sbjct: 541 KLLSPKPTSKIHSEPTYDNEKEFNFRPCDEQPVLSFDAAVQSVCQELDCIEEVPGDLFAE 600

Query: 601 TSDTSANSKNDFNNRVVTEIHEVTEASKPLSNGSSMNSTLNNGFHVDISATDFRYRGLEN 660
           TS   AN+K + NNRV TEIHEVTEASKPLSNGSS + T+NNGFH+DIS +DFRYRGLEN
Sbjct: 601 TSTMPANTKINSNNRVDTEIHEVTEASKPLSNGSSKSYTMNNGFHLDISTSDFRYRGLEN 660

Query: 661 GTTSEMCPEMKESAIRRETEGEFRLLGRREGNKHVGGRFFGLEESNIQSRGRRVSFRMEE 720
           GTTSE+CPE+KESAIRRETEGEFRLLGRREG+KHVGGRFFGLEESN+QSRGRRVSFRMEE
Sbjct: 661 GTTSEICPEVKESAIRRETEGEFRLLGRREGSKHVGGRFFGLEESNMQSRGRRVSFRMEE 720

Query: 721 NGKEHLSHNIEPGEVSVTSLDDEDYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNKT 780
           NGKEHLSHNI+PGEVSVTSLDD+DYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNKT
Sbjct: 721 NGKEHLSHNIDPGEVSVTSLDDDDYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNKT 780

Query: 781 TLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLIN 840
           TLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLIN
Sbjct: 781 TLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLIN 840

Query: 841 PEFVQKLAERDGISLGIGFLSHIWVLDSSKRQHGVLNLEESSLCRQTKNGRRGKHGFARL 900
           PEFVQKLAERDGISLGIGFLSHI VLDSSKRQ+GVLNLEESSLCR+TKNGRRGKHGFARL
Sbjct: 841 PEFVQKLAERDGISLGIGFLSHIRVLDSSKRQYGVLNLEESSLCRETKNGRRGKHGFARL 900

Query: 901 EVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLATVEEGSETT 948
           EVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLA VEEGSETT
Sbjct: 901 EVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLAPVEEGSETT 945

BLAST of Lsi10G006240 vs. ExPASy TrEMBL
Match: A0A0A0LMR8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G292770 PE=4 SV=1)

HSP 1 Score: 1776.9 bits (4601), Expect = 0.0e+00
Identity = 890/947 (93.98%), Postives = 918/947 (96.94%), Query Frame = 0

Query: 1   MHHSLWKPLSHCAALIMDKKSRKKDGSDSAMEMDIKKHKLILRKLEEHKLREALEEASED 60
           MHHSLWKPLSHCAALIMDKKSRKKDGSDSA  MDIKKHKLILRKLEEHKLREALEEASED
Sbjct: 1   MHHSLWKPLSHCAALIMDKKSRKKDGSDSA--MDIKKHKLILRKLEEHKLREALEEASED 60

Query: 61  GSLFKSQDVGSEPLPNDDNNGLGRSRSLARLQAQREFLKATAMAADRTYESDDAIPDLHE 120
           GSLFKSQDV SEPLPNDD+NGLGRSRSLARLQAQREFLKATAMAADRTYESDD IPDLHE
Sbjct: 61  GSLFKSQDVDSEPLPNDDSNGLGRSRSLARLQAQREFLKATAMAADRTYESDDDIPDLHE 120

Query: 121 AFSKFLTMYPKYQSSEKIDQLRSNEYSHLIKVCLDYCGFGLFSYVQSLHYWESSTFSLSE 180
           AFSKFLTMYPKYQSSEKIDQLRSNEYSHL+KVCLDYCGFGLFSYVQSLHYWESSTFSLSE
Sbjct: 121 AFSKFLTMYPKYQSSEKIDQLRSNEYSHLVKVCLDYCGFGLFSYVQSLHYWESSTFSLSE 180

Query: 181 IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFN 240
           IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFN
Sbjct: 181 IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFN 240

Query: 241 SNKKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKD 300
           +NKKLLTMFDYESQSVNW+AQCAR+KGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKD
Sbjct: 241 TNKKLLTMFDYESQSVNWLAQCARDKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKD 300

Query: 301 SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIIT 360
           SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIIT
Sbjct: 301 SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIIT 360

Query: 361 SFYRVFGYDPTGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSVDDLDRVGR 420
           SFYRVFGYDPTGFGCLLIK+SVMGSLQTRSGCTGSGMVKITPEYPMYLSDS+DDLD V R
Sbjct: 361 SFYRVFGYDPTGFGCLLIKKSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSMDDLDGVSR 420

Query: 421 FEDDQVAGVEDKTSETRQGLQLPAFSGAFTSAQVRDVYETEMDQDNSSDRDGTSTILEES 480
           FEDDQVAGV DKTSETRQG QLPAFSGAFTSAQVRDVYETEMD DNSSDRDGTSTILEES
Sbjct: 421 FEDDQVAGVVDKTSETRQGSQLPAFSGAFTSAQVRDVYETEMDHDNSSDRDGTSTILEES 480

Query: 481 ETISLGDVMKSPIFSEDESSDCSIWIDLGQSPLGSDNAGQSHKVEIASPLPQHWLKGKKN 540
           ETISLG+VMKSP+FSEDESSDCSIWIDLGQSPLGSDN GQ +K +IASPLPQHWLKG+KN
Sbjct: 481 ETISLGEVMKSPVFSEDESSDCSIWIDLGQSPLGSDNGGQMYKQKIASPLPQHWLKGRKN 540

Query: 541 KLLSPKPTSKIHSEPTYDNDKDFNLGPYDEQPVISFDAAVQSVCQELDCVEEVPRELFAE 600
           KLLSPKPTSKIHSEPTYDN+KDFN  P DEQPV+SFDAAVQSVCQELDCVEEVP+ELFAE
Sbjct: 541 KLLSPKPTSKIHSEPTYDNEKDFNFRPCDEQPVLSFDAAVQSVCQELDCVEEVPKELFAE 600

Query: 601 TSDTSANSKNDFNNRVVTEIHEVTEASKPLSNGSSMNSTLNNGFHVDISATDFRYRGLEN 660
            S   ANSK   NNRVVTEI EVTEASKPLSNGSS + T+NNGFH+DIS +DFRYRGLEN
Sbjct: 601 ASTMPANSKIISNNRVVTEIDEVTEASKPLSNGSSKSYTVNNGFHLDISTSDFRYRGLEN 660

Query: 661 GTTSEMCPEMKESAIRRETEGEFRLLGRREGNKHVGGRFFGLEESNIQSRGRRVSFRMEE 720
           GTTSE+CPE+KESAIRRETEGEFRLLGRR+G+KHVGGRFFGLE+SN+QSRGRRVSFRMEE
Sbjct: 661 GTTSEICPEVKESAIRRETEGEFRLLGRRDGSKHVGGRFFGLEDSNMQSRGRRVSFRMEE 720

Query: 721 NGKEHLSHNIEPGEVSVTSLDDEDYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNKT 780
           NGKE LSHNI+PGEVSVTSLDDEDYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNKT
Sbjct: 721 NGKEQLSHNIDPGEVSVTSLDDEDYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNKT 780

Query: 781 TLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLIN 840
           TLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLIN
Sbjct: 781 TLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLIN 840

Query: 841 PEFVQKLAERDGISLGIGFLSHIWVLDSSKRQHGVLNLEESSLCRQTKNGRRGKHGFARL 900
           PEFVQKLAERDGISLGIGFLSHI VLDSSKRQ+GVLNLEESSLCR+TKNGRRGKHGFARL
Sbjct: 841 PEFVQKLAERDGISLGIGFLSHIRVLDSSKRQYGVLNLEESSLCRETKNGRRGKHGFARL 900

Query: 901 EVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLATVEEGSETT 948
           EVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLA VEEGSETT
Sbjct: 901 EVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLAPVEEGSETT 945

BLAST of Lsi10G006240 vs. ExPASy TrEMBL
Match: A0A6J1DVI9 (uncharacterized protein LOC111024771 OS=Momordica charantia OX=3673 GN=LOC111024771 PE=4 SV=1)

HSP 1 Score: 1636.7 bits (4237), Expect = 0.0e+00
Identity = 831/948 (87.66%), Postives = 881/948 (92.93%), Query Frame = 0

Query: 1   MHHSLWKPLSHCAALIMDKKSRKKDGSDSAMEMDIKKHKLILRKLEEHKLREALEEASED 60
           MH+SLWKPLSHCAALIMDKKSRKKDGSDSA+E  IKK KLILRKLEEHKLREALEEASED
Sbjct: 1   MHYSLWKPLSHCAALIMDKKSRKKDGSDSAIE--IKKKKLILRKLEEHKLREALEEASED 60

Query: 61  GSLFKSQDVGSEPLPNDDNNGLGRSRSLARLQAQREFLKATAMAADRTYESDDAIPDLHE 120
           G LFKSQDVGS+P+P+     LGRSRSLARLQAQREFL+ATAMAADRTYESDDAIP+LHE
Sbjct: 61  GCLFKSQDVGSDPVPS-----LGRSRSLARLQAQREFLQATAMAADRTYESDDAIPELHE 120

Query: 121 AFSKFLTMYPKYQSSEKIDQLRSNEYSHLIKVCLDYCGFGLFSYVQSLHYWESSTFSLSE 180
           AFSKFLTMYPKY+SSE IDQLRSNEYSHL+KVCLDYCGFGLFSYVQ+LHYWESSTFSLSE
Sbjct: 121 AFSKFLTMYPKYESSEMIDQLRSNEYSHLMKVCLDYCGFGLFSYVQTLHYWESSTFSLSE 180

Query: 181 IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFN 240
           IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLL+ESYPF+
Sbjct: 181 IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLSESYPFH 240

Query: 241 SNKKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKD 300
           +NKKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKR+KKKD
Sbjct: 241 TNKKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKRKKKKD 300

Query: 301 -SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFII 360
            + GLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFII
Sbjct: 301 LAAGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFII 360

Query: 361 TSFYRVFGYDPTGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSVDDLDRVG 420
           TSFYRVFGYDPTGFGCLLIKRSVMGSLQT+SGCTGSGMVKITPEYPMYLSDS+DDLD +G
Sbjct: 361 TSFYRVFGYDPTGFGCLLIKRSVMGSLQTQSGCTGSGMVKITPEYPMYLSDSIDDLDGLG 420

Query: 421 RFEDDQVAGVEDKTSETRQGLQLPAFSGAFTSAQVRDVYETEMDQDNSSDRDGTSTILEE 480
           R EDD+VAGV D+T ETRQG QLPAFSGAFTSAQVRDV+ETEMD  N+SDRDGTSTI EE
Sbjct: 421 RIEDDEVAGVVDQTFETRQGSQLPAFSGAFTSAQVRDVFETEMDHGNNSDRDGTSTIFEE 480

Query: 481 SETISLGDVMKSPIFSEDESSDCSIWIDLGQSPLGSDNAGQSHKVEIASPLPQHWLKGKK 540
           SETISLG+VMKSP+FSEDESSDCSIWIDLGQSPLGSDNA Q +K +IASPLPQ+WL GKK
Sbjct: 481 SETISLGEVMKSPVFSEDESSDCSIWIDLGQSPLGSDNANQLNKQKIASPLPQYWLNGKK 540

Query: 541 NKLLSPKPTSKIHSEPTYDNDKDFNLGPYDEQPVISFDAAVQSVCQELDCVEEVPRELFA 600
           NKLLS KP SKIHS  TYD+ KDFN GPYDE  V+SFDAAVQSV QELD VEEVPREL A
Sbjct: 541 NKLLSHKPNSKIHSHLTYDDHKDFNSGPYDEHRVLSFDAAVQSVYQELDSVEEVPRELSA 600

Query: 601 ETSDTSANSKNDFNNRVVTEIHEVTEASKPLSNGSSMNSTLNNGFHVDISATDFRYRGLE 660
           ETS TS +S+ D + +V+TEIHEVTE  KPLSNGSS+NSTLNNGFH+  S          
Sbjct: 601 ETSATSVSSEKDSDTKVITEIHEVTETRKPLSNGSSINSTLNNGFHLSGS---------- 660

Query: 661 NGTTSEMCPEMKESAIRRETEGEFRLLGRREGNKHVGGRFFGLEESNIQSRGRRVSFRME 720
             +TSE+C E+KESAIRRETEGEFRLLGRREG KHVGGR FGLEE+++QSRGRRVSFRME
Sbjct: 661 -NSTSEICSEVKESAIRRETEGEFRLLGRREGTKHVGGRIFGLEETSMQSRGRRVSFRME 720

Query: 721 ENGKEHLSHNIEPGEVSVTSLDDEDYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNK 780
           ENGKE L+HN+E GEVSVTSLD+EDYTSNGEY DEEEWNRREPEIICRHLDHINMLGLNK
Sbjct: 721 ENGKEQLNHNVETGEVSVTSLDNEDYTSNGEYGDEEEWNRREPEIICRHLDHINMLGLNK 780

Query: 781 TTLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLI 840
           TTLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVR+RNRGLI
Sbjct: 781 TTLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRDRNRGLI 840

Query: 841 NPEFVQKLAERDGISLGIGFLSHIWVLDSSKRQHGVLNLEESSLCRQTKNGRRGKHGFAR 900
           NPEFVQKLAERDGISLGIGFLSHI VLDS +RQHGVLNLE+SSLCRQT+NGRRGK+GFAR
Sbjct: 841 NPEFVQKLAERDGISLGIGFLSHIRVLDSPRRQHGVLNLEDSSLCRQTENGRRGKNGFAR 900

Query: 901 LEVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLATVEEGSETT 948
           LEVVTASLGFLTNFEDVYKLW FVAKFLNPSFIREG LA VEEGSETT
Sbjct: 901 LEVVTASLGFLTNFEDVYKLWAFVAKFLNPSFIREGALAPVEEGSETT 930

BLAST of Lsi10G006240 vs. ExPASy TrEMBL
Match: A0A6J1I9D0 (uncharacterized protein LOC111470388 OS=Cucurbita maxima OX=3661 GN=LOC111470388 PE=4 SV=1)

HSP 1 Score: 1610.9 bits (4170), Expect = 0.0e+00
Identity = 819/948 (86.39%), Postives = 858/948 (90.51%), Query Frame = 0

Query: 1   MHHSLWKPLSHCAALIMDKKSRKKDGSDSAMEMDIKKHKLILRKLEEHKLREALEEASED 60
           MHHSLWKPLSHC ALIMDK+SR KDG DSA  MD+ KH++ILRKLEEHKLREALEEASED
Sbjct: 1   MHHSLWKPLSHCVALIMDKRSRTKDGYDSA--MDVNKHQMILRKLEEHKLREALEEASED 60

Query: 61  GSLFKSQDVGSEPLPND-DNNGLGRSRSLARLQAQREFLKATAMAADRTYESDDAIPDLH 120
           GSLFKSQ+V SEPL ND D NGLGRSRSLARLQAQREFLKATAMAADRTYESDDAIPDL 
Sbjct: 61  GSLFKSQNVDSEPLRNDGDENGLGRSRSLARLQAQREFLKATAMAADRTYESDDAIPDLR 120

Query: 121 EAFSKFLTMYPKYQSSEKIDQLRSNEYSHLIKVCLDYCGFGLFSYVQSLHYWESSTFSLS 180
           EAFSKFLTMYPKYQSSEKID+LRSNEYSHLIKVCLDYCGFGLFSYVQSLHYWESSTFSLS
Sbjct: 121 EAFSKFLTMYPKYQSSEKIDELRSNEYSHLIKVCLDYCGFGLFSYVQSLHYWESSTFSLS 180

Query: 181 EIAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPF 240
           EIAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPF
Sbjct: 181 EIAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPF 240

Query: 241 NSNKKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKK 300
           ++NKKLLTMFDYESQSVNWMAQ A+EKGAKAY+AWFKWP+LKLCSTDLRK+ITNKRRKKK
Sbjct: 241 HTNKKLLTMFDYESQSVNWMAQFAKEKGAKAYNAWFKWPSLKLCSTDLRKRITNKRRKKK 300

Query: 301 DSVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFII 360
           +SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFII
Sbjct: 301 ESVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFII 360

Query: 361 TSFYRVFGYDPTGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSVDDLDRVG 420
           TSFYRVFGYDPTGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYP+YLSDS+DDLD+VG
Sbjct: 361 TSFYRVFGYDPTGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYPVYLSDSIDDLDQVG 420

Query: 421 RFEDDQVAGVEDKTSETRQGLQLPAFSGAFTSAQVRDVYETEMDQDNSSDRDGTSTILEE 480
           RFEDD+VAGV DKTSETRQG QLPAFSGAFTSAQVRDV ETEMD DN SDRDGTSTILEE
Sbjct: 421 RFEDDRVAGVVDKTSETRQGSQLPAFSGAFTSAQVRDVLETEMDHDNISDRDGTSTILEE 480

Query: 481 SETISLGDVMKSPIFSEDESSDCSIWIDLGQSPLGSDNAGQSHKVEIASPLPQHWLKGKK 540
           SETISLG+VMKSP+FSEDESSDCSIWIDLGQSPLGSDNAGQ H  ++ASPLPQHWLKGKK
Sbjct: 481 SETISLGEVMKSPVFSEDESSDCSIWIDLGQSPLGSDNAGQLHTQKLASPLPQHWLKGKK 540

Query: 541 NKLLSPKPTSKIHSEPTYDNDKDFNLGPYDEQPVISFDAAVQSVCQELDCVEEVPRELFA 600
           NKLLSPKPTSKIHSEP+YD D DFN GPYD+ PV+SFDAAVQS CQELD V+EVPREL A
Sbjct: 541 NKLLSPKPTSKIHSEPSYDKDNDFNSGPYDDHPVLSFDAAVQSACQELDFVDEVPRELLA 600

Query: 601 ETSDTSANSKNDFNNRVVTEIHEVTEASKPLSNGSSMNSTLNNGFHVDISATDFRYRGLE 660
           ETS  SANSK D NNRVVTEIHE TEASKPLSNG+                         
Sbjct: 601 ETSAMSANSKKDSNNRVVTEIHEATEASKPLSNGA------------------------- 660

Query: 661 NGTTSEMCPEMKESAIRRETEGEFRLLGRREGNKHVGGRFFGLEESNIQSRGRRVSFRME 720
               SE+CPE KESAIRRETEGEFRLLGRREGNKHV                RRVSFRME
Sbjct: 661 ----SEICPETKESAIRRETEGEFRLLGRREGNKHV----------------RRVSFRME 720

Query: 721 ENGKEHLSHNIEPGEVSVTSLDDEDYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNK 780
           +NG EHL+H+IEPGEV++TSLDDEDYTSNGEY+DEE WNRREPEIICRHLDHINMLGLNK
Sbjct: 721 DNGNEHLNHSIEPGEVTMTSLDDEDYTSNGEYEDEETWNRREPEIICRHLDHINMLGLNK 780

Query: 781 TTLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLI 840
           TTLRLRFLINWLVTSLLQLKF  SEG+NK NLVQIYGPKIKYERGAAVAFNVRNRNRGLI
Sbjct: 781 TTLRLRFLINWLVTSLLQLKFQDSEGNNKANLVQIYGPKIKYERGAAVAFNVRNRNRGLI 840

Query: 841 NPEFVQKLAERDGISLGIGFLSHIWVLDSSKRQHGVLNLEESSLCRQTKNGRRGKHGFAR 900
           NPEFVQK+AERDGISLGIGFLSHI VLDS KRQ GVLNLEE SLC+Q +NGRRG+HGFAR
Sbjct: 841 NPEFVQKVAERDGISLGIGFLSHIRVLDSPKRQRGVLNLEEPSLCKQAENGRRGEHGFAR 900

Query: 901 LEVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLATVEEGSETT 948
           LEVVTASLGFLTNFEDVYKLW FVAKFLNPSFIREGTLA VEEGS+TT
Sbjct: 901 LEVVTASLGFLTNFEDVYKLWAFVAKFLNPSFIREGTLALVEEGSQTT 901

BLAST of Lsi10G006240 vs. NCBI nr
Match: XP_038902184.1 (uncharacterized protein LOC120088814 [Benincasa hispida])

HSP 1 Score: 1800.0 bits (4661), Expect = 0.0e+00
Identity = 901/947 (95.14%), Postives = 923/947 (97.47%), Query Frame = 0

Query: 1   MHHSLWKPLSHCAALIMDKKSRKKDGSDSAMEMDIKKHKLILRKLEEHKLREALEEASED 60
           MHHSLWKPLSHCAALIMDKKSRKKDGSDSA  MDIKKHKLILRKLEEHKLREALEEASED
Sbjct: 1   MHHSLWKPLSHCAALIMDKKSRKKDGSDSA--MDIKKHKLILRKLEEHKLREALEEASED 60

Query: 61  GSLFKSQDVGSEPLPNDDNNGLGRSRSLARLQAQREFLKATAMAADRTYESDDAIPDLHE 120
           GSLFKSQDV SEPLPNDDN+GLGRSRSLARLQAQREFLKATAMAADRTYESDDAIPDLHE
Sbjct: 61  GSLFKSQDVDSEPLPNDDNSGLGRSRSLARLQAQREFLKATAMAADRTYESDDAIPDLHE 120

Query: 121 AFSKFLTMYPKYQSSEKIDQLRSNEYSHLIKVCLDYCGFGLFSYVQSLHYWESSTFSLSE 180
           AFSKFLTMYPKYQSSEKIDQLRSNEYSHLIKVCLDYCGFGLFSYVQSLHYWESSTFSLSE
Sbjct: 121 AFSKFLTMYPKYQSSEKIDQLRSNEYSHLIKVCLDYCGFGLFSYVQSLHYWESSTFSLSE 180

Query: 181 IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFN 240
           IAANLSNQALYGGAERGT+EHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFN
Sbjct: 181 IAANLSNQALYGGAERGTIEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFN 240

Query: 241 SNKKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKD 300
           S+KKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKRR+KKD
Sbjct: 241 SSKKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRRKKD 300

Query: 301 SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIIT 360
           SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIIT
Sbjct: 301 SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIIT 360

Query: 361 SFYRVFGYDPTGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSVDDLDRVGR 420
           SFYRVFGYDPTGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYPMYLSDS+DDLDRVG+
Sbjct: 361 SFYRVFGYDPTGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSMDDLDRVGQ 420

Query: 421 FEDDQVAGVEDKTSETRQGLQLPAFSGAFTSAQVRDVYETEMDQDNSSDRDGTSTILEES 480
           FE DQVAGV DKTSETRQG QLPAFSGAFTSAQVRDVYETEMD DNSSDRDGTSTILEES
Sbjct: 421 FEGDQVAGVVDKTSETRQGSQLPAFSGAFTSAQVRDVYETEMDHDNSSDRDGTSTILEES 480

Query: 481 ETISLGDVMKSPIFSEDESSDCSIWIDLGQSPLGSDNAGQSHKVEIASPLPQHWLKGKKN 540
           ETISLG+VMKSP+FSEDE SDCSIWIDLGQSPLGSDNAGQSHK +I SPLPQHWLKGKKN
Sbjct: 481 ETISLGEVMKSPVFSEDELSDCSIWIDLGQSPLGSDNAGQSHKQKITSPLPQHWLKGKKN 540

Query: 541 KLLSPKPTSKIHSEPTYDNDKDFNLGPYDEQPVISFDAAVQSVCQELDCVEEVPRELFAE 600
           KLLSPKPTSKIHSEPTY+NDKDFNLGPYDEQPV+SFDAAV SVCQEL C+EEVPR++FAE
Sbjct: 541 KLLSPKPTSKIHSEPTYNNDKDFNLGPYDEQPVLSFDAAVHSVCQELGCIEEVPRDIFAE 600

Query: 601 TSDTSANSKNDFNNRVVTEIHEVTEASKPLSNGSSMNSTLNNGFHVDISATDFRYRGLEN 660
           TS TSANSKND   RVVTEIHEVTE SKPLSNG S+ STLNNGFH+DIS ++F YRGLEN
Sbjct: 601 TSATSANSKNDSPYRVVTEIHEVTEDSKPLSNGPSIKSTLNNGFHLDISTSEFCYRGLEN 660

Query: 661 GTTSEMCPEMKESAIRRETEGEFRLLGRREGNKHVGGRFFGLEESNIQSRGRRVSFRMEE 720
           GTTSE+CPEMKESAIRRETEGEFRLLGRREGNKHVGGRFFGLEESN+QSRGRRVSFRMEE
Sbjct: 661 GTTSEICPEMKESAIRRETEGEFRLLGRREGNKHVGGRFFGLEESNMQSRGRRVSFRMEE 720

Query: 721 NGKEHLSHNIEPGEVSVTSLDDEDYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNKT 780
           NGKEHLSHNIEPGEVSVTSLDDEDYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNKT
Sbjct: 721 NGKEHLSHNIEPGEVSVTSLDDEDYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNKT 780

Query: 781 TLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLIN 840
           TLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLIN
Sbjct: 781 TLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLIN 840

Query: 841 PEFVQKLAERDGISLGIGFLSHIWVLDSSKRQHGVLNLEESSLCRQTKNGRRGKHGFARL 900
           PEFVQKLAERDGISLGIGFLSHI VLDSSKRQHGVLNLEESSLCR TK+GR GKHGFARL
Sbjct: 841 PEFVQKLAERDGISLGIGFLSHIRVLDSSKRQHGVLNLEESSLCRHTKDGRHGKHGFARL 900

Query: 901 EVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLATVEEGSETT 948
           EVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLA+VEEGSETT
Sbjct: 901 EVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLASVEEGSETT 945

BLAST of Lsi10G006240 vs. NCBI nr
Match: XP_008457860.1 (PREDICTED: uncharacterized protein LOC103497444 [Cucumis melo] >KAA0045860.1 Pyridoxal phosphate-dependent transferases superfamily protein [Cucumis melo var. makuwa] >TYK13728.1 Pyridoxal phosphate-dependent transferases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 1777.3 bits (4602), Expect = 0.0e+00
Identity = 888/947 (93.77%), Postives = 919/947 (97.04%), Query Frame = 0

Query: 1   MHHSLWKPLSHCAALIMDKKSRKKDGSDSAMEMDIKKHKLILRKLEEHKLREALEEASED 60
           MHHSLWKPLSHCAALIMDKKSRKKDGSDSA  MDIKKHKLILRKLEEHKLREALEEASED
Sbjct: 1   MHHSLWKPLSHCAALIMDKKSRKKDGSDSA--MDIKKHKLILRKLEEHKLREALEEASED 60

Query: 61  GSLFKSQDVGSEPLPNDDNNGLGRSRSLARLQAQREFLKATAMAADRTYESDDAIPDLHE 120
           GSL KSQDV SEPLPNDDNNGLGRSRSLARLQAQREFLKATAMAADRTYESD  IPDLHE
Sbjct: 61  GSLSKSQDVDSEPLPNDDNNGLGRSRSLARLQAQREFLKATAMAADRTYESDGDIPDLHE 120

Query: 121 AFSKFLTMYPKYQSSEKIDQLRSNEYSHLIKVCLDYCGFGLFSYVQSLHYWESSTFSLSE 180
           AFSKFLTMYPKYQSSEKIDQLRSNEYSHL+KVCLDYCGFGLFSYVQSLHYWESSTFSLSE
Sbjct: 121 AFSKFLTMYPKYQSSEKIDQLRSNEYSHLVKVCLDYCGFGLFSYVQSLHYWESSTFSLSE 180

Query: 181 IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFN 240
           IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFN
Sbjct: 181 IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFN 240

Query: 241 SNKKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKD 300
           +NKKLLTMFDYESQSVNWMAQCAR+KGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKD
Sbjct: 241 TNKKLLTMFDYESQSVNWMAQCARDKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKD 300

Query: 301 SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIIT 360
           SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIIT
Sbjct: 301 SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIIT 360

Query: 361 SFYRVFGYDPTGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSVDDLDRVGR 420
           SFYRVFGYDPTGFGCLLIK+SVMGSLQTRSGCTGSGMVKITPEYPMYLSDS+DDLD VG+
Sbjct: 361 SFYRVFGYDPTGFGCLLIKKSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSMDDLDGVGQ 420

Query: 421 FEDDQVAGVEDKTSETRQGLQLPAFSGAFTSAQVRDVYETEMDQDNSSDRDGTSTILEES 480
           FEDDQVAGV DKTSETRQG QLPAFSGAFTSAQVRD+YETEMD DNSSDRDGTSTILEES
Sbjct: 421 FEDDQVAGVVDKTSETRQGSQLPAFSGAFTSAQVRDIYETEMDHDNSSDRDGTSTILEES 480

Query: 481 ETISLGDVMKSPIFSEDESSDCSIWIDLGQSPLGSDNAGQSHKVEIASPLPQHWLKGKKN 540
           ETISLG+VMKSP+FSEDESSDCSIWIDLGQSPLGSDN GQ +K +IASPLPQHWLKG+KN
Sbjct: 481 ETISLGEVMKSPVFSEDESSDCSIWIDLGQSPLGSDNGGQLYKQKIASPLPQHWLKGRKN 540

Query: 541 KLLSPKPTSKIHSEPTYDNDKDFNLGPYDEQPVISFDAAVQSVCQELDCVEEVPRELFAE 600
           KLLSPKPTSKIHSEPTYDN+K+FN  P DEQPV+SFDAAVQSVCQELDC+EEVP +LFAE
Sbjct: 541 KLLSPKPTSKIHSEPTYDNEKEFNFRPCDEQPVLSFDAAVQSVCQELDCIEEVPGDLFAE 600

Query: 601 TSDTSANSKNDFNNRVVTEIHEVTEASKPLSNGSSMNSTLNNGFHVDISATDFRYRGLEN 660
           TS   AN+K + NNRV TEIHEVTEASKPLSNGSS + T+NNGFH+DIS +DFRYRGLEN
Sbjct: 601 TSTMPANTKINSNNRVDTEIHEVTEASKPLSNGSSKSYTMNNGFHLDISTSDFRYRGLEN 660

Query: 661 GTTSEMCPEMKESAIRRETEGEFRLLGRREGNKHVGGRFFGLEESNIQSRGRRVSFRMEE 720
           GTTSE+CPE+KESAIRRETEGEFRLLGRREG+KHVGGRFFGLEESN+QSRGRRVSFRMEE
Sbjct: 661 GTTSEICPEVKESAIRRETEGEFRLLGRREGSKHVGGRFFGLEESNMQSRGRRVSFRMEE 720

Query: 721 NGKEHLSHNIEPGEVSVTSLDDEDYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNKT 780
           NGKEHLSHNI+PGEVSVTSLDD+DYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNKT
Sbjct: 721 NGKEHLSHNIDPGEVSVTSLDDDDYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNKT 780

Query: 781 TLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLIN 840
           TLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLIN
Sbjct: 781 TLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLIN 840

Query: 841 PEFVQKLAERDGISLGIGFLSHIWVLDSSKRQHGVLNLEESSLCRQTKNGRRGKHGFARL 900
           PEFVQKLAERDGISLGIGFLSHI VLDSSKRQ+GVLNLEESSLCR+TKNGRRGKHGFARL
Sbjct: 841 PEFVQKLAERDGISLGIGFLSHIRVLDSSKRQYGVLNLEESSLCRETKNGRRGKHGFARL 900

Query: 901 EVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLATVEEGSETT 948
           EVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLA VEEGSETT
Sbjct: 901 EVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLAPVEEGSETT 945

BLAST of Lsi10G006240 vs. NCBI nr
Match: XP_004148049.1 (uncharacterized protein LOC101209057 [Cucumis sativus] >KGN62047.1 hypothetical protein Csa_006632 [Cucumis sativus])

HSP 1 Score: 1776.9 bits (4601), Expect = 0.0e+00
Identity = 890/947 (93.98%), Postives = 918/947 (96.94%), Query Frame = 0

Query: 1   MHHSLWKPLSHCAALIMDKKSRKKDGSDSAMEMDIKKHKLILRKLEEHKLREALEEASED 60
           MHHSLWKPLSHCAALIMDKKSRKKDGSDSA  MDIKKHKLILRKLEEHKLREALEEASED
Sbjct: 1   MHHSLWKPLSHCAALIMDKKSRKKDGSDSA--MDIKKHKLILRKLEEHKLREALEEASED 60

Query: 61  GSLFKSQDVGSEPLPNDDNNGLGRSRSLARLQAQREFLKATAMAADRTYESDDAIPDLHE 120
           GSLFKSQDV SEPLPNDD+NGLGRSRSLARLQAQREFLKATAMAADRTYESDD IPDLHE
Sbjct: 61  GSLFKSQDVDSEPLPNDDSNGLGRSRSLARLQAQREFLKATAMAADRTYESDDDIPDLHE 120

Query: 121 AFSKFLTMYPKYQSSEKIDQLRSNEYSHLIKVCLDYCGFGLFSYVQSLHYWESSTFSLSE 180
           AFSKFLTMYPKYQSSEKIDQLRSNEYSHL+KVCLDYCGFGLFSYVQSLHYWESSTFSLSE
Sbjct: 121 AFSKFLTMYPKYQSSEKIDQLRSNEYSHLVKVCLDYCGFGLFSYVQSLHYWESSTFSLSE 180

Query: 181 IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFN 240
           IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFN
Sbjct: 181 IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFN 240

Query: 241 SNKKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKD 300
           +NKKLLTMFDYESQSVNW+AQCAR+KGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKD
Sbjct: 241 TNKKLLTMFDYESQSVNWLAQCARDKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKD 300

Query: 301 SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIIT 360
           SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIIT
Sbjct: 301 SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIIT 360

Query: 361 SFYRVFGYDPTGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSVDDLDRVGR 420
           SFYRVFGYDPTGFGCLLIK+SVMGSLQTRSGCTGSGMVKITPEYPMYLSDS+DDLD V R
Sbjct: 361 SFYRVFGYDPTGFGCLLIKKSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSMDDLDGVSR 420

Query: 421 FEDDQVAGVEDKTSETRQGLQLPAFSGAFTSAQVRDVYETEMDQDNSSDRDGTSTILEES 480
           FEDDQVAGV DKTSETRQG QLPAFSGAFTSAQVRDVYETEMD DNSSDRDGTSTILEES
Sbjct: 421 FEDDQVAGVVDKTSETRQGSQLPAFSGAFTSAQVRDVYETEMDHDNSSDRDGTSTILEES 480

Query: 481 ETISLGDVMKSPIFSEDESSDCSIWIDLGQSPLGSDNAGQSHKVEIASPLPQHWLKGKKN 540
           ETISLG+VMKSP+FSEDESSDCSIWIDLGQSPLGSDN GQ +K +IASPLPQHWLKG+KN
Sbjct: 481 ETISLGEVMKSPVFSEDESSDCSIWIDLGQSPLGSDNGGQMYKQKIASPLPQHWLKGRKN 540

Query: 541 KLLSPKPTSKIHSEPTYDNDKDFNLGPYDEQPVISFDAAVQSVCQELDCVEEVPRELFAE 600
           KLLSPKPTSKIHSEPTYDN+KDFN  P DEQPV+SFDAAVQSVCQELDCVEEVP+ELFAE
Sbjct: 541 KLLSPKPTSKIHSEPTYDNEKDFNFRPCDEQPVLSFDAAVQSVCQELDCVEEVPKELFAE 600

Query: 601 TSDTSANSKNDFNNRVVTEIHEVTEASKPLSNGSSMNSTLNNGFHVDISATDFRYRGLEN 660
            S   ANSK   NNRVVTEI EVTEASKPLSNGSS + T+NNGFH+DIS +DFRYRGLEN
Sbjct: 601 ASTMPANSKIISNNRVVTEIDEVTEASKPLSNGSSKSYTVNNGFHLDISTSDFRYRGLEN 660

Query: 661 GTTSEMCPEMKESAIRRETEGEFRLLGRREGNKHVGGRFFGLEESNIQSRGRRVSFRMEE 720
           GTTSE+CPE+KESAIRRETEGEFRLLGRR+G+KHVGGRFFGLE+SN+QSRGRRVSFRMEE
Sbjct: 661 GTTSEICPEVKESAIRRETEGEFRLLGRRDGSKHVGGRFFGLEDSNMQSRGRRVSFRMEE 720

Query: 721 NGKEHLSHNIEPGEVSVTSLDDEDYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNKT 780
           NGKE LSHNI+PGEVSVTSLDDEDYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNKT
Sbjct: 721 NGKEQLSHNIDPGEVSVTSLDDEDYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNKT 780

Query: 781 TLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLIN 840
           TLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLIN
Sbjct: 781 TLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLIN 840

Query: 841 PEFVQKLAERDGISLGIGFLSHIWVLDSSKRQHGVLNLEESSLCRQTKNGRRGKHGFARL 900
           PEFVQKLAERDGISLGIGFLSHI VLDSSKRQ+GVLNLEESSLCR+TKNGRRGKHGFARL
Sbjct: 841 PEFVQKLAERDGISLGIGFLSHIRVLDSSKRQYGVLNLEESSLCRETKNGRRGKHGFARL 900

Query: 901 EVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLATVEEGSETT 948
           EVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLA VEEGSETT
Sbjct: 901 EVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLAPVEEGSETT 945

BLAST of Lsi10G006240 vs. NCBI nr
Match: XP_022158238.1 (uncharacterized protein LOC111024771 [Momordica charantia])

HSP 1 Score: 1636.7 bits (4237), Expect = 0.0e+00
Identity = 831/948 (87.66%), Postives = 881/948 (92.93%), Query Frame = 0

Query: 1   MHHSLWKPLSHCAALIMDKKSRKKDGSDSAMEMDIKKHKLILRKLEEHKLREALEEASED 60
           MH+SLWKPLSHCAALIMDKKSRKKDGSDSA+E  IKK KLILRKLEEHKLREALEEASED
Sbjct: 1   MHYSLWKPLSHCAALIMDKKSRKKDGSDSAIE--IKKKKLILRKLEEHKLREALEEASED 60

Query: 61  GSLFKSQDVGSEPLPNDDNNGLGRSRSLARLQAQREFLKATAMAADRTYESDDAIPDLHE 120
           G LFKSQDVGS+P+P+     LGRSRSLARLQAQREFL+ATAMAADRTYESDDAIP+LHE
Sbjct: 61  GCLFKSQDVGSDPVPS-----LGRSRSLARLQAQREFLQATAMAADRTYESDDAIPELHE 120

Query: 121 AFSKFLTMYPKYQSSEKIDQLRSNEYSHLIKVCLDYCGFGLFSYVQSLHYWESSTFSLSE 180
           AFSKFLTMYPKY+SSE IDQLRSNEYSHL+KVCLDYCGFGLFSYVQ+LHYWESSTFSLSE
Sbjct: 121 AFSKFLTMYPKYESSEMIDQLRSNEYSHLMKVCLDYCGFGLFSYVQTLHYWESSTFSLSE 180

Query: 181 IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFN 240
           IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLL+ESYPF+
Sbjct: 181 IAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLSESYPFH 240

Query: 241 SNKKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKD 300
           +NKKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKR+KKKD
Sbjct: 241 TNKKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKRKKKKD 300

Query: 301 -SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFII 360
            + GLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFII
Sbjct: 301 LAAGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFII 360

Query: 361 TSFYRVFGYDPTGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSVDDLDRVG 420
           TSFYRVFGYDPTGFGCLLIKRSVMGSLQT+SGCTGSGMVKITPEYPMYLSDS+DDLD +G
Sbjct: 361 TSFYRVFGYDPTGFGCLLIKRSVMGSLQTQSGCTGSGMVKITPEYPMYLSDSIDDLDGLG 420

Query: 421 RFEDDQVAGVEDKTSETRQGLQLPAFSGAFTSAQVRDVYETEMDQDNSSDRDGTSTILEE 480
           R EDD+VAGV D+T ETRQG QLPAFSGAFTSAQVRDV+ETEMD  N+SDRDGTSTI EE
Sbjct: 421 RIEDDEVAGVVDQTFETRQGSQLPAFSGAFTSAQVRDVFETEMDHGNNSDRDGTSTIFEE 480

Query: 481 SETISLGDVMKSPIFSEDESSDCSIWIDLGQSPLGSDNAGQSHKVEIASPLPQHWLKGKK 540
           SETISLG+VMKSP+FSEDESSDCSIWIDLGQSPLGSDNA Q +K +IASPLPQ+WL GKK
Sbjct: 481 SETISLGEVMKSPVFSEDESSDCSIWIDLGQSPLGSDNANQLNKQKIASPLPQYWLNGKK 540

Query: 541 NKLLSPKPTSKIHSEPTYDNDKDFNLGPYDEQPVISFDAAVQSVCQELDCVEEVPRELFA 600
           NKLLS KP SKIHS  TYD+ KDFN GPYDE  V+SFDAAVQSV QELD VEEVPREL A
Sbjct: 541 NKLLSHKPNSKIHSHLTYDDHKDFNSGPYDEHRVLSFDAAVQSVYQELDSVEEVPRELSA 600

Query: 601 ETSDTSANSKNDFNNRVVTEIHEVTEASKPLSNGSSMNSTLNNGFHVDISATDFRYRGLE 660
           ETS TS +S+ D + +V+TEIHEVTE  KPLSNGSS+NSTLNNGFH+  S          
Sbjct: 601 ETSATSVSSEKDSDTKVITEIHEVTETRKPLSNGSSINSTLNNGFHLSGS---------- 660

Query: 661 NGTTSEMCPEMKESAIRRETEGEFRLLGRREGNKHVGGRFFGLEESNIQSRGRRVSFRME 720
             +TSE+C E+KESAIRRETEGEFRLLGRREG KHVGGR FGLEE+++QSRGRRVSFRME
Sbjct: 661 -NSTSEICSEVKESAIRRETEGEFRLLGRREGTKHVGGRIFGLEETSMQSRGRRVSFRME 720

Query: 721 ENGKEHLSHNIEPGEVSVTSLDDEDYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNK 780
           ENGKE L+HN+E GEVSVTSLD+EDYTSNGEY DEEEWNRREPEIICRHLDHINMLGLNK
Sbjct: 721 ENGKEQLNHNVETGEVSVTSLDNEDYTSNGEYGDEEEWNRREPEIICRHLDHINMLGLNK 780

Query: 781 TTLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLI 840
           TTLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVR+RNRGLI
Sbjct: 781 TTLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRDRNRGLI 840

Query: 841 NPEFVQKLAERDGISLGIGFLSHIWVLDSSKRQHGVLNLEESSLCRQTKNGRRGKHGFAR 900
           NPEFVQKLAERDGISLGIGFLSHI VLDS +RQHGVLNLE+SSLCRQT+NGRRGK+GFAR
Sbjct: 841 NPEFVQKLAERDGISLGIGFLSHIRVLDSPRRQHGVLNLEDSSLCRQTENGRRGKNGFAR 900

Query: 901 LEVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLATVEEGSETT 948
           LEVVTASLGFLTNFEDVYKLW FVAKFLNPSFIREG LA VEEGSETT
Sbjct: 901 LEVVTASLGFLTNFEDVYKLWAFVAKFLNPSFIREGALAPVEEGSETT 930

BLAST of Lsi10G006240 vs. NCBI nr
Match: XP_023513272.1 (uncharacterized protein LOC111777789 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1615.9 bits (4183), Expect = 0.0e+00
Identity = 821/948 (86.60%), Postives = 861/948 (90.82%), Query Frame = 0

Query: 1   MHHSLWKPLSHCAALIMDKKSRKKDGSDSAMEMDIKKHKLILRKLEEHKLREALEEASED 60
           MHHSLWKPLSHC ALIMDKKSR KDG DSA  MDIKKH++ILRKLEEHKLREALEEASED
Sbjct: 1   MHHSLWKPLSHCVALIMDKKSRTKDGYDSA--MDIKKHQMILRKLEEHKLREALEEASED 60

Query: 61  GSLFKSQDVGSEPLPND-DNNGLGRSRSLARLQAQREFLKATAMAADRTYESDDAIPDLH 120
           GSLFKSQ+V SEPL ND D+NGLGRSRSLARLQAQREFLKATAMAADRTYESDDAIPDL 
Sbjct: 61  GSLFKSQNVDSEPLRNDGDDNGLGRSRSLARLQAQREFLKATAMAADRTYESDDAIPDLR 120

Query: 121 EAFSKFLTMYPKYQSSEKIDQLRSNEYSHLIKVCLDYCGFGLFSYVQSLHYWESSTFSLS 180
           EAFSKFLTMYPKYQSSEKID+LRSNEYSHLIKVCLDYCGFGLFSYVQSLHYWESSTFSLS
Sbjct: 121 EAFSKFLTMYPKYQSSEKIDELRSNEYSHLIKVCLDYCGFGLFSYVQSLHYWESSTFSLS 180

Query: 181 EIAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPF 240
           EIAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPF
Sbjct: 181 EIAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPF 240

Query: 241 NSNKKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKK 300
           ++NKKLLTMFDYESQSVNWMAQ A+EKGAKAY+AWFKWP+LKLCSTDLRK+ITNKRRKKK
Sbjct: 241 HTNKKLLTMFDYESQSVNWMAQFAKEKGAKAYNAWFKWPSLKLCSTDLRKRITNKRRKKK 300

Query: 301 DSVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFII 360
           +SVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFII
Sbjct: 301 ESVGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFII 360

Query: 361 TSFYRVFGYDPTGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSVDDLDRVG 420
           TSFYRVFGYDPTGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYP+YLSDS+DDLD+VG
Sbjct: 361 TSFYRVFGYDPTGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYPVYLSDSIDDLDQVG 420

Query: 421 RFEDDQVAGVEDKTSETRQGLQLPAFSGAFTSAQVRDVYETEMDQDNSSDRDGTSTILEE 480
           RFEDD+VAGV DKTSETRQG QLPAFSGAFTSAQVRDV ETEMD DN SDRDGTSTILEE
Sbjct: 421 RFEDDRVAGVVDKTSETRQGSQLPAFSGAFTSAQVRDVLETEMDHDNISDRDGTSTILEE 480

Query: 481 SETISLGDVMKSPIFSEDESSDCSIWIDLGQSPLGSDNAGQSHKVEIASPLPQHWLKGKK 540
           SETISLG+VMKSP+FSEDESSDCSIWIDLGQSPLGSDNAGQ H  ++ASPLPQHWLKGKK
Sbjct: 481 SETISLGEVMKSPVFSEDESSDCSIWIDLGQSPLGSDNAGQLHTQKLASPLPQHWLKGKK 540

Query: 541 NKLLSPKPTSKIHSEPTYDNDKDFNLGPYDEQPVISFDAAVQSVCQELDCVEEVPRELFA 600
           NKLLSPKPTSKIHSEP+YD D DFN GPYD+ PV+SFDAAVQS CQE+DC++EVPREL A
Sbjct: 541 NKLLSPKPTSKIHSEPSYDKDNDFNSGPYDDHPVLSFDAAVQSACQEIDCIKEVPRELLA 600

Query: 601 ETSDTSANSKNDFNNRVVTEIHEVTEASKPLSNGSSMNSTLNNGFHVDISATDFRYRGLE 660
           ETS  SANSK D NN+VVTEIHE TEASKPLSNG+                         
Sbjct: 601 ETSAMSANSKKDSNNQVVTEIHEATEASKPLSNGA------------------------- 660

Query: 661 NGTTSEMCPEMKESAIRRETEGEFRLLGRREGNKHVGGRFFGLEESNIQSRGRRVSFRME 720
               SE+C E KESAIRRETEGEFRLLGRREGNKHV                RRVSFRME
Sbjct: 661 ----SEICSETKESAIRRETEGEFRLLGRREGNKHV----------------RRVSFRME 720

Query: 721 ENGKEHLSHNIEPGEVSVTSLDDEDYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNK 780
           +NG EHL+H+IEPGEV++TSLDDEDYTSNGEYDDEE WNRREPEIICRHLDHINMLGLNK
Sbjct: 721 DNGNEHLNHSIEPGEVTMTSLDDEDYTSNGEYDDEETWNRREPEIICRHLDHINMLGLNK 780

Query: 781 TTLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLI 840
           TTLRLRFLINWLVTSLLQLKF GSEG+NK NLVQIYGPKIKYERGAAVAFNVRNRNRGLI
Sbjct: 781 TTLRLRFLINWLVTSLLQLKFQGSEGNNKANLVQIYGPKIKYERGAAVAFNVRNRNRGLI 840

Query: 841 NPEFVQKLAERDGISLGIGFLSHIWVLDSSKRQHGVLNLEESSLCRQTKNGRRGKHGFAR 900
           NPEFVQK+AERDGISLGIGFLSHI VLDS K Q GVLNLEESSLC+Q +NGRRG+HGFAR
Sbjct: 841 NPEFVQKVAERDGISLGIGFLSHIRVLDSPKWQRGVLNLEESSLCKQAENGRRGEHGFAR 900

Query: 901 LEVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLATVEEGSETT 948
           LEVVTASLGFLTNFEDVYKLW FVAKFLNPSFIREGTLA VEEGS+TT
Sbjct: 901 LEVVTASLGFLTNFEDVYKLWAFVAKFLNPSFIREGTLALVEEGSQTT 901

BLAST of Lsi10G006240 vs. TAIR 10
Match: AT2G23520.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 1122.5 bits (2902), Expect = 0.0e+00
Identity = 607/955 (63.56%), Postives = 738/955 (77.28%), Query Frame = 0

Query: 1   MHHSLWKPLSHCAALIMDK-KSRKKDGSDSAMEMDIKKHKLILRKLEEHKLREALEEASE 60
           MH  LWK + HCA LI+DK KSR++DGSDS   +D+++   +LRKL E KLR+ALEEASE
Sbjct: 1   MHFPLWKQIHHCATLILDKSKSRRRDGSDS--PIDVRRKASMLRKLYEDKLRDALEEASE 60

Query: 61  DGSLFKSQDVGSEPLPNDDNNGLGRSRSLARLQAQREFLKATAMAADRTYESDDAIPDLH 120
           +GSLFKSQDV +E    + +  LGRSRSLARL AQREFL+ATA+AA+R +ES+D IP+L 
Sbjct: 61  NGSLFKSQDVENE----NQDESLGRSRSLARLHAQREFLRATALAAERAFESEDDIPELL 120

Query: 121 EAFSKFLTMYPKYQSSEKIDQLRSNEYSHLI--KVCLDYCGFGLFSYVQSLHYWESSTFS 180
           EAF+KFLTMYPK+++SEK+DQLRS+EY HL+  KVCLDYCGFGLFSYVQ+LHYW+S TFS
Sbjct: 121 EAFNKFLTMYPKFETSEKVDQLRSDEYGHLLDSKVCLDYCGFGLFSYVQTLHYWDSCTFS 180

Query: 181 LSEIAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESY 240
           LSEI ANLSN ALYGGAE GTVEHD+K+RIMD+LNIPE EYGLVFT SRGSAF+LLAESY
Sbjct: 181 LSEITANLSNHALYGGAEIGTVEHDLKTRIMDYLNIPESEYGLVFTGSRGSAFRLLAESY 240

Query: 241 PFNSNKKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRK 300
           PF++NK+LLTMFD+ESQSVNWMAQ AREKGAKAY+AWFKWPTLKLCSTDL+K++++K+RK
Sbjct: 241 PFHTNKRLLTMFDHESQSVNWMAQTAREKGAKAYNAWFKWPTLKLCSTDLKKRLSHKKRK 300

Query: 301 KKDS-VGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPD 360
           KKDS VGLFVFP QSRVTG+KYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRP+
Sbjct: 301 KKDSAVGLFVFPAQSRVTGSKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPE 360

Query: 361 FIITSFYRVFGYDPTGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSVDDLD 420
           FIITSFY+VFG+DPTGFGCLLIK+SVMG+LQ++SG TGSG+VKITP+YP+YLSDS+D LD
Sbjct: 361 FIITSFYKVFGHDPTGFGCLLIKKSVMGNLQSQSGKTGSGIVKITPQYPLYLSDSIDGLD 420

Query: 421 RVGRFEDDQVAGVEDK---TSETRQGLQLPAFSGAFTSAQVRDVYETEMDQDNSSDRDGT 480
            +   ED  +    DK   T   R+G Q+P FSGA+TSAQVRDV+ET++ +DN+SDRDGT
Sbjct: 421 GLVGLEDHDIGTNGDKPATTDAARRGAQMPVFSGAYTSAQVRDVFETDLLEDNASDRDGT 480

Query: 481 -STILEESETISLGDVMKSPIFSEDESSDCSIWIDLGQSPLGSDNAGQSHKVEIASPLPQ 540
            STI EE+E++S+G++MKSP FSEDESSD S WIDLGQSPLGSD+AG  +  +IASPLP 
Sbjct: 481 SSTIFEENESVSVGELMKSPAFSEDESSDNSFWIDLGQSPLGSDSAGHLNHHKIASPLPP 540

Query: 541 HWLKGKKNKLLSPKPTSKIHSEPTYDNDKDFNLGPYDEQPVISFDAAVQSVCQELDCVEE 600
            W   K+    SPKP +K +S P YD            + V+SFDAAV SV QE++    
Sbjct: 541 FWFTSKRQ---SPKPVAKSYSSPMYDG-----------KDVLSFDAAVMSVTQEIN---- 600

Query: 601 VPRELFAETSDTSANSKNDFNNRVVTEIHEVTEASKPLSNGSSMNSTLNNGFHVDISATD 660
                    S  S N +N  NN  + EI E    +     GS   S              
Sbjct: 601 ---------STPSRNLRNS-NNLQIQEIQEENCGNIVYRAGSGFGS-------------- 660

Query: 661 FRYRGLENGTTSEMCPEMKESAIRRETEGEFRLLGRREGNKHVGGRFFGLEESNIQSRGR 720
                  NG++S++  +MK++AIRRETEGEFRLLGRR      GGR  GLE+    SRG 
Sbjct: 661 -------NGSSSKISSDMKDNAIRRETEGEFRLLGRR----GTGGRLLGLEDEQ-PSRGT 720

Query: 721 RVSFRMEENGKEHLSHNIEPGEVSVTSLDDEDYTSNGEYDDEEEWNRREPEIICRHLDHI 780
           RVSF M     + +SH+++ GE S+ S+ DE   S+GE  +E++W+RREPEI+C H+DH+
Sbjct: 721 RVSFNM-----DRVSHSLDQGEASLASVYDE---SDGENPNEDDWDRREPEIVCSHIDHV 780

Query: 781 NMLGLNKTTLRLRFLINWLVTSLLQLKF--PGSEGSNK-VNLVQIYGPKIKYERGAAVAF 840
           NMLGLNKTT RLRFLINWLV SLLQLK   PGS+GS++ +NLVQIYGPKIKYERGAAVAF
Sbjct: 781 NMLGLNKTTSRLRFLINWLVISLLQLKVPEPGSDGSSRYMNLVQIYGPKIKYERGAAVAF 840

Query: 841 NVRNRNRGLINPEFVQKLAERDGISLGIGFLSHIWVLDSSKRQHGVLNL-EESSLCRQTK 900
           NV+++++G ++PE V KLAER+G+SLGIG LSHI ++D  +   G   + E+SSL  Q +
Sbjct: 841 NVKDKSKGFVSPEIVLKLAEREGVSLGIGILSHIRIMDLPRNHRGGARIKEDSSLHLQRE 887

Query: 901 NGRR-GKHGFARLEVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLATVEE 943
            G+R GK+GF R EVVTASL FL+NFEDVYKLW FVAKFLNP F REG+L TV E
Sbjct: 901 AGKRGGKNGFVRFEVVTASLSFLSNFEDVYKLWAFVAKFLNPGFSREGSLPTVIE 887

BLAST of Lsi10G006240 vs. TAIR 10
Match: AT4G37100.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 1099.7 bits (2843), Expect = 0.0e+00
Identity = 614/968 (63.43%), Postives = 728/968 (75.21%), Query Frame = 0

Query: 1   MHHSLWKPLSHCAALIMDKKS---RKKDGSDSAMEMDIKKHKLILRKLEEHKLREALEEA 60
           MH SLWK + HCA+LI+DK     R++DGSDS+  +++KK   ++RKL E KLREALEEA
Sbjct: 1   MHFSLWKQIHHCASLILDKSKSSRRRRDGSDSS--LNVKKKAALIRKLYEDKLREALEEA 60

Query: 61  SEDGSLFKSQDVGSEPLPNDDNNGLGRSRSLARLQAQREFLKATAMAADRTYESDDAIPD 120
           SE+GSLFKSQD+  +    + +  LGRSRSLARL AQREFL+ATA+AA+R  ES+D+IP+
Sbjct: 61  SENGSLFKSQDIDQD----NGDGSLGRSRSLARLHAQREFLRATALAAERIIESEDSIPE 120

Query: 121 LHEAFSKFLTMYPKYQSSEKIDQLRSNEYSHL----IKVCLDYCGFGLFSYVQSLHYWES 180
           L EA +KFL+MYPKYQ+SEKIDQLRS+EYSHL     KVCLDYCGFGLFSYVQ+LHYW++
Sbjct: 121 LREALTKFLSMYPKYQASEKIDQLRSDEYSHLSSSASKVCLDYCGFGLFSYVQTLHYWDT 180

Query: 181 STFSLSEIAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLL 240
            TFSLSEI ANLSN ALYGGAE GTVEHDIK+RIMD+LNIPE+EYGLVFTVSRGSAF+LL
Sbjct: 181 CTFSLSEITANLSNHALYGGAESGTVEHDIKTRIMDYLNIPENEYGLVFTVSRGSAFRLL 240

Query: 241 AESYPFNSNKKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITN 300
           AESYPF SNK+LLTMFD+ESQSVNWMAQ AREKGAKAY+AWFKWPTLKLCSTDL+K+++ 
Sbjct: 241 AESYPFQSNKRLLTMFDHESQSVNWMAQTAREKGAKAYNAWFKWPTLKLCSTDLKKRLSY 300

Query: 301 KRRKKKDS-VGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSL 360
           K+RKKKDS VGLFVFP QSRVTG KYSYQWMALAQQN+WHVLLDAGSLGPKDMDSLGLSL
Sbjct: 301 KKRKKKDSAVGLFVFPAQSRVTGTKYSYQWMALAQQNHWHVLLDAGSLGPKDMDSLGLSL 360

Query: 361 FRPDFIITSFYRVFGYDPTGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSV 420
           FRP+FIITSFYRVFG+DPTGFGCLLIK+SVMGSLQ++SG TGSG+VKITPEYP+YLSDSV
Sbjct: 361 FRPEFIITSFYRVFGHDPTGFGCLLIKKSVMGSLQSQSGKTGSGIVKITPEYPLYLSDSV 420

Query: 421 DDLDRVGRFEDDQVAGVEDKTSET-RQGLQLPAFSGAFTSAQVRDVYETEMDQDN-SSDR 480
           D LD +  FED      +DKT E  R G Q+PAFSGA+TSAQVRDV+ETE+ +DN SSDR
Sbjct: 421 DGLDGLVGFEDHN----DDKTKEAHRPGTQMPAFSGAYTSAQVRDVFETELLEDNISSDR 480

Query: 481 DGT--STILEESETISLGDVMKSPIFSEDESSDCSIWIDLGQSPLGSDNAGQSHKVEIAS 540
           DGT  +TI EE+E++S+G++MKSP+FSEDESSD S WIDLGQSPLGSD        +IAS
Sbjct: 481 DGTTSTTIFEETESVSVGELMKSPVFSEDESSDNSFWIDLGQSPLGSDQHN-----KIAS 540

Query: 541 PLPQHWL--KGKKNKLLSPKPTSKIHSEPTYDNDKDFNLGPYDEQPVISFDAAVQSVCQE 600
           PLP  WL  K K+ +  SPKP  K +S P YD +            V+SFDAAV SV + 
Sbjct: 541 PLPPIWLTNKRKQKQRQSPKPIPKSYSSPLYDGN-----------DVLSFDAAVMSVTEH 600

Query: 601 LDCVEEVPRELFAETSDTSANSKNDFNNRVVTEIHEVTEASKPLSNGSSMNSTLNNGFHV 660
                          S  S N ++  N+  V EI E            +   +  NG   
Sbjct: 601 ------------GTNSTPSRNRRSSSNHLHVQEIQE-----------ENCGHSFANGL-- 660

Query: 661 DISATDFRYRGLENGTTSEMCPEMKESAIRRETEGEFRLLGRREGNKHVGGRFFGLEESN 720
                           +S +  E+KESAIRRETEGEFRLLG R+G +    R  G+E+ +
Sbjct: 661 ---------------KSSNISSEIKESAIRRETEGEFRLLGGRDGGR---SRLLGVEDEH 720

Query: 721 IQSRGRRVSFRMEENGKEHLSHNI-EPGEVSVTSLDDEDY--TSNGEYDDEE----EWNR 780
             S+GRRVSF M     E +SH+I EPGE S+ S+ DEDY  TS+ E  D+E    EW+R
Sbjct: 721 -PSKGRRVSFNM-----ERVSHSIVEPGEASLASVYDEDYINTSDVENGDDEGADDEWDR 780

Query: 781 R--EPEIICRHLDHINMLGLNKTTLRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGP 840
           R  E EI+CRH+DH+NMLGLNKTT RLRFLINWLV SLLQL+ P S G   +NLVQIYGP
Sbjct: 781 RDTETEIVCRHIDHVNMLGLNKTTTRLRFLINWLVISLLQLQVPES-GGRHMNLVQIYGP 840

Query: 841 KIKYERGAAVAFNVRNRNRGLINPEFVQKLAERDGISLGIGFLSHIWVLDSSKRQHGVLN 900
           KIKYERGAAVAFNVR++++G ++PE VQ+L +R+G+SLGIG LSHI ++D   R H    
Sbjct: 841 KIKYERGAAVAFNVRDKSKGFVSPEIVQRLGDREGVSLGIGILSHIRIVDEKPRNHRART 889

Query: 901 LEESSLCRQTKNGRRGKHGFARLEVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTL 946
            E+S+L  Q +    GK+GF R EVVTASL FLTNFEDVYKLW FVAKFLNP F REG+L
Sbjct: 901 KEDSALHLQNE---AGKNGFIRFEVVTASLSFLTNFEDVYKLWVFVAKFLNPGFSREGSL 889

BLAST of Lsi10G006240 vs. TAIR 10
Match: AT5G66950.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 1012.3 bits (2616), Expect = 2.6e-295
Identity = 567/954 (59.43%), Postives = 700/954 (73.38%), Query Frame = 0

Query: 1   MHHSLWKPLSHC-AALIMDKKSRKKDGSDSAMEMDIKKHKLILRKLEEHKLREALEEASE 60
           MH SLWKP+ HC AAL++DKKS     S S      +   +  RKL E KLREALE+ASE
Sbjct: 1   MHISLWKPIYHCAAALVLDKKSSGSSSSSS------RNRDVTQRKLHESKLREALEQASE 60

Query: 61  DGSLFKSQDVGSEPLPNDDNNGLGRSRSLARLQAQREFLKATAMAADRTYESDDAIPDLH 120
           DG L KSQD+  E    D    LGRSRSLARL AQREFL+AT++AA R +ES++ +P+L 
Sbjct: 61  DGLLVKSQDMEEEDESQDQI--LGRSRSLARLNAQREFLRATSLAAQRAFESEETLPELE 120

Query: 121 EAFSKFLTMYPKYQSSEKIDQLRSNEYSHLI--KVCLDYCGFGLFSYVQSLHYWESSTFS 180
           EA + FLTMYPKYQSSEK+D+LR++EY HL   KVCLDYCGFGLFSY+Q++HYW++ TFS
Sbjct: 121 EALTIFLTMYPKYQSSEKVDELRNDEYFHLSLPKVCLDYCGFGLFSYLQTVHYWDTCTFS 180

Query: 181 LSEIAANLSNQALYGGAERGTVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESY 240
           LSEI+ANLSN A+YGGAE+G++EHDIK RIMD+LNIPE+EYGLVFTVSRGSAFKLLAESY
Sbjct: 181 LSEISANLSNHAIYGGAEKGSIEHDIKIRIMDYLNIPENEYGLVFTVSRGSAFKLLAESY 240

Query: 241 PFNSNKKLLTMFDYESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRK 300
           PF++NKKLLTMFD+ESQSV+WM QCA+EKGAK  SAWFKWPTL+LCS DL+K+I +K+++
Sbjct: 241 PFHTNKKLLTMFDHESQSVSWMGQCAKEKGAKVGSAWFKWPTLRLCSMDLKKEILSKKKR 300

Query: 301 KKDS-VGLFVFPVQSRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPD 360
           KKDS  GLFVFPVQSRVTG+KYSYQWMALAQQNNWHVLLDAG+LGPKDMDSLGLSLFRPD
Sbjct: 301 KKDSATGLFVFPVQSRVTGSKYSYQWMALAQQNNWHVLLDAGALGPKDMDSLGLSLFRPD 360

Query: 361 FIITSFYRVFGYDPTGFGCLLIKRSVMGSLQTRSGCTGSGMVKITPEYPMYLSDSVDDLD 420
           FIITSFYRVFGYDPTGFGCLLIK+SV+  LQ++SG T SG+VKITPEYP+YLSDS+D L+
Sbjct: 361 FIITSFYRVFGYDPTGFGCLLIKKSVISCLQSQSGKTSSGIVKITPEYPLYLSDSMDGLE 420

Query: 421 RVGRFEDDQVAGVEDKTSETRQGLQLPAFSGAFTSAQVRDVYETEMDQDNSSDRDGTSTI 480
            +   +D+ +A   D  +    G QLPAFSGA+TSAQV+DV+ET+MD +  SDRD TS +
Sbjct: 421 GLTGIQDNGIAINGDNKA---LGTQLPAFSGAYTSAQVQDVFETDMDHEIGSDRDNTSAV 480

Query: 481 LEESETISLGDVMKSPIFSEDESSDCSIWIDLGQSPLGSDNAGQSHKVEIASPLPQHWLK 540
            EE+E+IS+G+++KSP+FSEDESSD S+WIDLGQSP  SDNAG  +K +  SPL    ++
Sbjct: 481 FEEAESISVGELIKSPVFSEDESSDSSLWIDLGQSPADSDNAGHLNKQK--SPL---LVR 540

Query: 541 GKKNKLLSPKPTSKIHSEPTYDNDKDFNLGPYDEQPVISFDAAVQSVCQELDCVEEVPRE 600
               +  SPKP SK             N G    + V+SFDAAV SV  E+   EEV  E
Sbjct: 541 KNHKRRSSPKPASKA------------NNGSNGGRHVLSFDAAVLSVSHEVG--EEVIEE 600

Query: 601 LFAETSDTSANSKNDFNNRV-VTEIHEVTEASKPLSNGSSMNSTLNNGFHVDISATDFRY 660
                 + S  ++ D + R+ VTEI E  E       G S   T +              
Sbjct: 601 ------ENSEMNQIDTSRRLRVTEIEEEEE------EGGSSKLTAH-------------- 660

Query: 661 RGLENGTTSEMCPEMKESAIRRETEGEFRLLGRREGNKHVGGRFFGLEESNIQSRGRRVS 720
               NG++S     +K+SAIRRETEGEFRLLGRRE +++ GGR    E+ +     RRVS
Sbjct: 661 ---ANGSSS----GIKDSAIRRETEGEFRLLGRREKSQYNGGRLLVNEDEHPSK--RRVS 720

Query: 721 FRMEENGKEHLSHNIEPGEVSVTSLDDEDYTSNGEYDDEEEWNRREPEIICRHLDHINML 780
           FR           +++ GE SV SL DED   +G    E + ++REPEI+CRH+DH+NML
Sbjct: 721 FR-----------SVDHGEASVISLGDEDEEEDGSNGVEWDDDQREPEIVCRHIDHVNML 780

Query: 781 GLNKTTLRLRFLINWLVTSLLQLKFP--GSEGSNKVNLVQIYGPKIKYERGAAVAFNVRN 840
           GLNKTT RLR+LINWLVTSLLQL+ P   S+G +K NLVQIYGPKIKYERG++VAFN+R+
Sbjct: 781 GLNKTTSRLRYLINWLVTSLLQLRLPRSDSDGEHK-NLVQIYGPKIKYERGSSVAFNIRD 840

Query: 841 RNRGLINPEFVQKLAERDGISLGIGFLSHIWVLDSSKRQHGVLNLEESSLCRQTKNGRRG 900
              G+++PE VQKLAER+GISLGIG+LSHI ++D+          + SS     + GR  
Sbjct: 841 LKSGMVHPEIVQKLAEREGISLGIGYLSHIKIIDNRSE-------DSSSWKPVDREGR-- 868

Query: 901 KHGFARLEVVTASLGFLTNFEDVYKLWGFVAKFLNPSFIREGTLATVEEGSETT 948
            +GF R+EVVTASLGFLTNFEDVY+LW FVAKFL+P F ++GTL TV E  +++
Sbjct: 901 NNGFIRVEVVTASLGFLTNFEDVYRLWNFVAKFLSPGFAKQGTLPTVIEEDDSS 868

BLAST of Lsi10G006240 vs. TAIR 10
Match: AT5G51920.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 295.0 bits (754), Expect = 2.1e-79
Identity = 149/326 (45.71%), Postives = 211/326 (64.72%), Query Frame = 0

Query: 94  QREFLKAT--AMAADRTYESDDAIPDLHEAFSKFLTMYPKYQSSEKIDQLRSNEYSHL-- 153
           +R F + T   +  D  +   +++P   E+FS F+  YP Y  + KID+LRS+ Y HL  
Sbjct: 46  RRNFAQTTVSTIFPDTEFTDPNSLPSHQESFSDFIQAYPNYSDTYKIDRLRSDHYFHLGL 105

Query: 154 -IKVCLDYCGFGLFSYVQSLHY-----------WESSTFSLSEIAANLSNQALYGGAERG 213
               CLDY G GL+SY Q L+Y            ES  FS+S    NL  + L  G +  
Sbjct: 106 SHYTCLDYIGIGLYSYSQLLNYDPSTYQISSSLSESPFFSVSPKIGNLKEKLLNDGGQET 165

Query: 214 TVEHDIKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFNSNKKLLTMFDYESQSVN 273
             E+ +K RIM  L I E +Y +VFT +R SAF+L+AESYPFNS +KLLT++DYES++V+
Sbjct: 166 EFEYSMKRRIMGFLKISEEDYSMVFTANRTSAFRLVAESYPFNSKRKLLTVYDYESEAVS 225

Query: 274 WMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQIT-NKRRKKKDSVGLFVFPVQSRVTGA 333
            + + + ++GAK  +A F WP LKLCS+ LRK +T  K   K    G++VFP+ SRVTG+
Sbjct: 226 EINRVSEKRGAKVAAAEFSWPRLKLCSSKLRKLVTAGKNGSKTKKKGIYVFPLHSRVTGS 285

Query: 334 KYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIITSFYRVFGYDPTGFGCL 393
           +Y Y WM++AQ+N WHV++DA  LGPKDMDS GLS++ PDF++ SFY+VFG +P+GFGCL
Sbjct: 286 RYPYLWMSVAQENGWHVMIDACGLGPKDMDSFGLSIYNPDFMVCSFYKVFGENPSGFGCL 345

Query: 394 LIKRSVMGSLQTRSGCTGSGMVKITP 403
            +K+S +  L++    TG GM+ + P
Sbjct: 346 FVKKSTISILES---STGPGMINLVP 368


HSP 2 Score: 121.3 bits (303), Expect = 4.1e-27
Identity = 84/211 (39.81%), Postives = 110/211 (52.13%), Query Frame = 0

Query: 726 LSHNIEPGEVSVTSLDD----EDYTSNGEYDDEEEWNRREPEIICRHLDHINMLGLNKTT 785
           L  +  PG +++   D+         N    D EE       +  + LDH++ LGL  T 
Sbjct: 355 LESSTGPGMINLVPTDNPISLHALEINRTQTDSEETYSFSSSVEYKGLDHVDSLGLVATG 414

Query: 786 LRLRFLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLINP 845
            R R LINWLV++L +LK      S    LV+IYGPK+ + RG AVAFN+ N     I P
Sbjct: 415 NRSRCLINWLVSALYKLKH-----STTSRLVKIYGPKVNFNRGPAVAFNLFNHKGEKIEP 474

Query: 846 EFVQKLAERDGISLGIGFLSHIWVLDSSKRQHGVLNLEESSLCRQTKNGRRGKHGFARLE 905
             VQKLAE   ISLG  FL +I   +      GV +       R  +  R       R+ 
Sbjct: 475 FIVQKLAECSNISLGKSFLKNILFQED---YEGVKD-------RVFEKKRNRDVDEPRIS 534

Query: 906 VVTASLGFLTNFEDVYKLWGFVAKFLNPSFI 933
           V+TA+LGFL NFEDVYKLW FVA+FL+  F+
Sbjct: 535 VLTAALGFLANFEDVYKLWIFVARFLDSEFV 550

BLAST of Lsi10G006240 vs. TAIR 10
Match: AT4G22980.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 13 plant structures; EXPRESSED DURING: 9 growth stages; BEST Arabidopsis thaliana protein match is: Pyridoxal phosphate (PLP)-dependent transferases superfamily protein (TAIR:AT5G51920.1); Has 520 Blast hits to 468 proteins in 130 species: Archae - 5; Bacteria - 23; Metazoa - 99; Fungi - 131; Plants - 231; Viruses - 0; Other Eukaryotes - 31 (source: NCBI BLink). )

HSP 1 Score: 220.3 bits (560), Expect = 6.5e-57
Identity = 126/312 (40.38%), Postives = 184/312 (58.97%), Query Frame = 0

Query: 85  SRSLARLQAQREFLKATA----MAADRTYESDDAIPDLHEAFSKFLTMYPKYQSSEKIDQ 144
           S S++    + EF   T     +  +  + S +++P L  +F   +T +P Y  + + D 
Sbjct: 24  SHSMSEKPEELEFSVTTTGTSFLTRNTKFTSQESLPRLRTSFYDLITAFPDYLQTNQADH 83

Query: 145 LRSNEYSHLIKVCLDYCGFG----LFSYVQSLHYWESSTFSLSEIAANLSNQALYGGAER 204
           LRS EY +L         FG    LFSY Q     ES +  L+     LS + +  G E 
Sbjct: 84  LRSTEYQNLSS---SSHVFGQQQPLFSYSQFREISESES-DLNHSLLTLSCKQVSSGKEL 143

Query: 205 GTVEHD------IKSRIMDHLNIPEHEYGLVFTVSRGSAFKLLAESYPFNSNKKLLTMFD 264
            + E +      I+ RI   +N+ E EY ++ T  R SAFK++AE Y F +N  LLT+++
Sbjct: 144 LSFEEESRFQSRIRKRITSFMNLEESEYHMILTQDRSSAFKIVAELYSFKTNPNLLTVYN 203

Query: 265 YESQSVNWMAQCAREKGAKAYSAWFKWPTLKLCSTDLRKQITNKRRKKKDSVGLFVFPVQ 324
           YE ++V  M + + +KG K  SA F WP+ ++ S  L+++IT  +R+ K   GLFVFP+Q
Sbjct: 204 YEDEAVEEMIRISEKKGIKPQSAEFSWPSTEILSEKLKRRITRSKRRGKR--GLFVFPLQ 263

Query: 325 SRVTGAKYSYQWMALAQQNNWHVLLDAGSLGPKDMDSLGLSLFRPDFIITSFYRVFGY-D 382
           S VTGA YSY WM+LA+++ WHVLLD  +LG KDM++LGLSLF+PDF+I SF  V G  D
Sbjct: 264 SLVTGASYSYSWMSLARESEWHVLLDTSALGSKDMETLGLSLFQPDFLICSFTEVLGQDD 323


HSP 2 Score: 99.4 bits (246), Expect = 1.7e-20
Identity = 68/210 (32.38%), Postives = 114/210 (54.29%), Query Frame = 0

Query: 731 EPGEVSVTSLDDEDY----TSNGEYDDEEEWNRREPEII-CRHLDHINMLGLNKTTLRLR 790
           + G   +T +D ED+    TS+ E  + E   +++  +I  + LDH + LGL   + R +
Sbjct: 360 QAGYNEITPVDHEDHKAASTSSSEIVEIESSVKQDKAMIEFQGLDHADSLGLILISRRSK 419

Query: 791 FLINWLVTSLLQLKFPGSEGSNKVNLVQIYGPKIKYERGAAVAFNVRNRNRGLINPEFVQ 850
            L  WL+ +L  L+ PG     ++ LV++YGPK K  RG +++FN+ +     ++P  V+
Sbjct: 420 SLTLWLLRALRTLQHPGYH-QTEMPLVKLYGPKTKPSRGPSISFNIFDWQGEKVDPLMVE 479

Query: 851 KLAERDGISLGIGFLSHIWVLDSSKRQHGVLNLEESSLCRQTKNGRRGKHGFARLEVVTA 910
           +LAER+ I L   +L H + + + +R    ++L                    RL VVT 
Sbjct: 480 RLAEREKIGLRCAYL-HKFRIGNKRRSDEAVSL--------------------RLSVVTV 539

Query: 911 SL-GFLTNFEDVYKLWGFVAKFLNPSFIRE 935
            L GF+TNFEDV+K+W FV++FL+  F+ +
Sbjct: 540 RLGGFMTNFEDVFKVWEFVSRFLDADFVEK 547

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q16P903.2e-1629.37Molybdenum cofactor sulfurase 3 OS=Aedes aegypti OX=7159 GN=mal3 PE=3 SV=1[more]
Q16GH04.2e-1628.62Molybdenum cofactor sulfurase 1 OS=Aedes aegypti OX=7159 GN=mal1 PE=3 SV=1[more]
Q9C5X87.1e-1625.76Molybdenum cofactor sulfurase OS=Arabidopsis thaliana OX=3702 GN=ABA3 PE=1 SV=1[more]
Q8LGM72.7e-1525.17Molybdenum cofactor sulfurase OS=Solanum lycopersicum OX=4081 GN=FLACCA PE=2 SV=... [more]
Q7QFL73.0e-1428.43Molybdenum cofactor sulfurase OS=Anopheles gambiae OX=7165 GN=mal PE=3 SV=5[more]
Match NameE-valueIdentityDescription
A0A5D3CRB40.0e+0093.77Pyridoxal phosphate-dependent transferases superfamily protein OS=Cucumis melo v... [more]
A0A1S3C7520.0e+0093.77uncharacterized protein LOC103497444 OS=Cucumis melo OX=3656 GN=LOC103497444 PE=... [more]
A0A0A0LMR80.0e+0093.98Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G292770 PE=4 SV=1[more]
A0A6J1DVI90.0e+0087.66uncharacterized protein LOC111024771 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A6J1I9D00.0e+0086.39uncharacterized protein LOC111470388 OS=Cucurbita maxima OX=3661 GN=LOC111470388... [more]
Match NameE-valueIdentityDescription
XP_038902184.10.0e+0095.14uncharacterized protein LOC120088814 [Benincasa hispida][more]
XP_008457860.10.0e+0093.77PREDICTED: uncharacterized protein LOC103497444 [Cucumis melo] >KAA0045860.1 Pyr... [more]
XP_004148049.10.0e+0093.98uncharacterized protein LOC101209057 [Cucumis sativus] >KGN62047.1 hypothetical ... [more]
XP_022158238.10.0e+0087.66uncharacterized protein LOC111024771 [Momordica charantia][more]
XP_023513272.10.0e+0086.60uncharacterized protein LOC111777789 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G23520.10.0e+0063.56Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT4G37100.10.0e+0063.43Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT5G66950.12.6e-29559.43Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT5G51920.12.1e-7945.71Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT4G22980.16.5e-5740.38FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 88..108
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 460..482
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 61..84
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 541..560
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 728..754
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 460..476
NoneNo IPR availablePANTHERPTHR14237:SF14PYRIDOXAL PHOSPHATE (PLP)-DEPENDENT TRANSFERASES SUPERFAMILY PROTEINcoord: 34..946
NoneNo IPR availablePANTHERPTHR14237MOLYBDOPTERIN COFACTOR SULFURASE MOSCcoord: 34..946
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 175..444
e-value: 1.9E-18
score: 68.5
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 189..391

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G006240.1Lsi10G006240.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016740 transferase activity
molecular_function GO:0003824 catalytic activity