Lsi10G005700 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi10G005700 vs. ExPASy Swiss-Prot
Match: Q9ZQE6 (Calmodulin-like protein 30 OS=Arabidopsis thaliana OX=3702 GN=CML30 PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 8.0e-31
Identity = 74/156 (47.44%), Postives = 102/156 (65.38%), Query Frame = 0

Query: 31  KQRSNSSSASSKHF-RPNLEEIKWAFDKFDSNKDGKISFEEYKAAHRGLAGSKEISDAEA 90
           +Q S  SS     F +P++ E++  F +FD +KDGKIS  EYK   R L   + I D   
Sbjct: 30  RQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLDKDGKISQTEYKVVLRALGQERAIED--V 89

Query: 91  AKSFKFVDADGDGFVDLKEFVELYTMSGGEVRVGDIESAFKVYDSNGDGKISAEEVMEIM 150
            K FK VD DGDGF+D +EF++ Y  SGG +R  DI ++F  +D NGDGKISAEEVM ++
Sbjct: 90  PKIFKAVDLDGDGFIDFREFIDAYKRSGG-IRSSDIRNSFWTFDLNGDGKISAEEVMSVL 149

Query: 151 NTLGERTTVKACRQMVKGVDMDGDGFIDIHEFSKLM 186
             LGER +++ C +MV+ VD DGDG +++ EF K+M
Sbjct: 150 WKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKMM 182

BLAST of Lsi10G005700 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 114.4 bits (285), Expect = 1.5e-24
Identity = 62/144 (43.06%), Postives = 92/144 (63.89%), Query Frame = 0

Query: 47  NLEEIKWAFDKFDSNKDGKISFEEYKAAHRGLAGSKEISDAEAAKSFKFVDADGDGFVDL 106
           ++++IK  F +FD N DGKIS +E K   R L  S   S  E     K  D DG+GF+DL
Sbjct: 14  SMDDIKKVFQRFDKNGDGKISVDELKEVIRAL--SPTASPEETVTMMKQFDLDGNGFIDL 73

Query: 107 KEFVELYTM---SGGEVR--VGDIESAFKVYDSNGDGKISAEEVMEIMNTLGERTTVKAC 166
            EFV L+ +    GG  R  V D++ AF++YD +G+G+ISA+E+  +M  LGE+ +V+ C
Sbjct: 74  DEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDC 133

Query: 167 RQMVKGVDMDGDGFIDIHEFSKLM 186
           ++M+  VD+DGDG ++  EF K+M
Sbjct: 134 KKMISKVDIDGDGCVNFDEFKKMM 155

BLAST of Lsi10G005700 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 4.7e-23
Identity = 59/148 (39.86%), Postives = 88/148 (59.46%), Query Frame = 0

Query: 41  SKHFRPNLEEIKWAFDKFDSNKDGKISFEEYKAAHRGLAGSKEISDAEAAKSFKFVDADG 100
           S++   ++E+IK  F +FD N DGKIS +E K     L  S   S  E     K  D DG
Sbjct: 6   SRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGAL--SPNASQEETKAMMKEFDLDG 65

Query: 101 DGFVDLKEFVELYTM---SGGEVRVGDIESAFKVYDSNGDGKISAEEVMEIMNTLGERTT 160
           +GF+DL EFV L+ +   S     + D++ AF +YD + +G+ISA E+  +M  LGE+ +
Sbjct: 66  NGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCS 125

Query: 161 VKACRQMVKGVDMDGDGFIDIHEFSKLM 186
           ++ C++M+  VD DGDG +D  EF K+M
Sbjct: 126 IQDCQRMINKVDSDGDGCVDFEEFKKMM 151

BLAST of Lsi10G005700 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 8.8e-22
Identity = 63/161 (39.13%), Postives = 92/161 (57.14%), Query Frame = 0

Query: 27  STQIKQRSNSSSASSKHFRPNLEEIKWAFDKFDSNKDGKISFEEYKAAHRGLAGSKEISD 86
           S  + Q   +S   S    P  EE++  F KFD+N DG+IS  E  A    L  +   +D
Sbjct: 18  SPPLPQADPASGGGSPAPTPE-EEMERVFRKFDANGDGRISRSELGALFESLGHA--ATD 77

Query: 87  AEAAKSFKFVDADGDGFVDLKEFVELYTMSGGEVRV--GDIESAFKVYDSNGDGKISAEE 146
            E A+     DADGDGF+ L EF  L   + G+      D+  AF+V+D++G+G ISA E
Sbjct: 78  DELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVFDADGNGTISAAE 137

Query: 147 VMEIMNTLGERTTVKACRQMVKGVDMDGDGFIDIHEFSKLM 186
           +  +++ LGE+ TV+ CR+M++GVD +GDG I   EF  +M
Sbjct: 138 LARVLHGLGEKATVQQCRRMIEGVDQNGDGLISFEEFKVMM 175

BLAST of Lsi10G005700 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 8.8e-22
Identity = 67/177 (37.85%), Postives = 104/177 (58.76%), Query Frame = 0

Query: 18  KRQPKNIPSSTQIKQRSNSS-SASSKHFRPNLEEIKWAFDKFDSNKDGKISFEEYKAAHR 77
           K Q  N  SS+ +   ++S     ++  +  + E++  F KFD N DGKIS +E  A   
Sbjct: 4   KNQGSNGGSSSNVGIGADSPYLQKARSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMT 63

Query: 78  GLAGSKEISDAEAAKSFKFVDADGDGFVDLKEFVELYT--MSGGEVRVGDIESAFKVYDS 137
            L    E+ + E  K+   +D  GDG+++ +EFVEL T  M   +V + +++ AF VYD 
Sbjct: 64  SL--GHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDV-LENLKDAFSVYDI 123

Query: 138 NGDGKISAEEVMEIMNTLGERTTVKACRQMVKGVDMDGDGFIDIHEFSKLMGMYSSK 192
           +G+G ISAEE+ E++ +LG+  ++  CR+M+ GVD DGDG ID  EF  +M M S +
Sbjct: 124 DGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMGSRR 177

BLAST of Lsi10G005700 vs. ExPASy TrEMBL
Match: A0A0A0LMP3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G286450 PE=4 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 1.1e-80
Identity = 159/189 (84.13%), Postives = 168/189 (88.89%), Query Frame = 0

Query: 6   SSVLRLFTSCSRKRQPKNIPSSTQIKQRSNSSSASSKHFRPNLEEIKWAFDKFDSNKDGK 65
           SS+L+LF SCS KR  KN   S  IK+RSNSSS SSKHFRPNLEEIKWAF+KFDSNKDGK
Sbjct: 2   SSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHFRPNLEEIKWAFEKFDSNKDGK 61

Query: 66  ISFEEYKAAHRGLAGSKEISDAEAAKSFKFVDADGDGFVDLKEFVELYTMSGGEVRVGDI 125
           ISFEEYK AHRGLAGSKEI+DAEA KSFK VD DGDGFVDLKEFVELYTMS GEV+VGDI
Sbjct: 62  ISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGDI 121

Query: 126 ESAFKVYDSNGDGKISAEEVMEIMNTLGERTTVKACRQMVKGVDMDGDGFIDIHEFSKLM 185
           ESAFKVYDSNGDGKISAEEVM IM  LGE TT+KAC+QMVKGVDMDGDGFID+ EFSKLM
Sbjct: 122 ESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKLM 181

Query: 186 GMYSSKFQN 195
           G YSSKFQN
Sbjct: 182 GKYSSKFQN 190

BLAST of Lsi10G005700 vs. ExPASy TrEMBL
Match: A0A5D3BJJ3 (Putative calcium-binding protein CML32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G00260 PE=4 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.3e-79
Identity = 158/188 (84.04%), Postives = 167/188 (88.83%), Query Frame = 0

Query: 6   SSVLRLFTSCSRKRQPKNIPSSTQIKQRSNSSSASSKHFRPNLEEIKWAFDKFDSNKDGK 65
           +S+L+LF SCS KR  KN   S QIK+RSNSSS SSKHFRPNLEEIKWAF+KFDSNKDGK
Sbjct: 2   TSLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDGK 61

Query: 66  ISFEEYKAAHRGLAGSKEISDAEAAKSFKFVDADGDGFVDLKEFVELYTMSGGEVRVGDI 125
           ISFEEYK AHRGLAGSKEI+DAEA KSFK VDADGDGFVDLKEFVELYTMS GEV+VGDI
Sbjct: 62  ISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGDI 121

Query: 126 ESAFKVYDSNGDGKISAEEVMEIMNTLGERTTVKACRQMVKGVDMDGDGFIDIHEFSKLM 185
           ESAFKVYDSNGDGKISAEEVM IM  LGE TT+ AC+QMVKGVDMDGDGFIDI EFSKLM
Sbjct: 122 ESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKLM 181

Query: 186 GMYSSKFQ 194
             YSSKFQ
Sbjct: 182 DTYSSKFQ 189

BLAST of Lsi10G005700 vs. ExPASy TrEMBL
Match: A0A1S3C6F1 (probable calcium-binding protein CML32 OS=Cucumis melo OX=3656 GN=LOC103497422 PE=4 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.3e-79
Identity = 158/188 (84.04%), Postives = 167/188 (88.83%), Query Frame = 0

Query: 6   SSVLRLFTSCSRKRQPKNIPSSTQIKQRSNSSSASSKHFRPNLEEIKWAFDKFDSNKDGK 65
           +S+L+LF SCS KR  KN   S QIK+RSNSSS SSKHFRPNLEEIKWAF+KFDSNKDGK
Sbjct: 2   TSLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDGK 61

Query: 66  ISFEEYKAAHRGLAGSKEISDAEAAKSFKFVDADGDGFVDLKEFVELYTMSGGEVRVGDI 125
           ISFEEYK AHRGLAGSKEI+DAEA KSFK VDADGDGFVDLKEFVELYTMS GEV+VGDI
Sbjct: 62  ISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGDI 121

Query: 126 ESAFKVYDSNGDGKISAEEVMEIMNTLGERTTVKACRQMVKGVDMDGDGFIDIHEFSKLM 185
           ESAFKVYDSNGDGKISAEEVM IM  LGE TT+ AC+QMVKGVDMDGDGFIDI EFSKLM
Sbjct: 122 ESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKLM 181

Query: 186 GMYSSKFQ 194
             YSSKFQ
Sbjct: 182 DTYSSKFQ 189

BLAST of Lsi10G005700 vs. ExPASy TrEMBL
Match: A0A6J1G5E9 (calmodulin-like protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111450966 PE=4 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 1.4e-78
Identity = 161/194 (82.99%), Postives = 169/194 (87.11%), Query Frame = 0

Query: 1   MTNKGSSVLRLFTSCSRKRQPKNIPSSTQIKQRSNS---SSASSKHFRPNLEEIKWAFDK 60
           M NKG SVL LFTSCS KRQ K IPSSTQ KQRS S   SSASSKH R NLEEIKW FDK
Sbjct: 1   MKNKGRSVLSLFTSCSPKRQLKQIPSSTQPKQRSKSISDSSASSKHVRVNLEEIKWVFDK 60

Query: 61  FDSNKDGKISFEEYKAAHRGLAGSKEISDAEAAKSFKFVDADGDGFVDLKEFVELYTMSG 120
           FD+NKDGKISFEEYKAAH  ++GSKEISDAEAAKSFK VDADGDGFV+LKEFVELYTMSG
Sbjct: 61  FDTNKDGKISFEEYKAAHGAMSGSKEISDAEAAKSFKLVDADGDGFVELKEFVELYTMSG 120

Query: 121 GEVRVGDIESAFKVYDSNGDGKISAEEVMEIMNTLGERTTVKACRQMVKGVDMDGDGFID 180
           GEV++GDI+SAFKVYDSNGDGKISAEEVMEIM  LGE TT+KACRQMVKGVD DGDGFID
Sbjct: 121 GEVKMGDIQSAFKVYDSNGDGKISAEEVMEIMKILGESTTLKACRQMVKGVDKDGDGFID 180

Query: 181 IHEFSKLMGMYSSK 192
           I EFS LMG YS K
Sbjct: 181 IDEFSSLMGSYSFK 194

BLAST of Lsi10G005700 vs. ExPASy TrEMBL
Match: A0A6J1L0C4 (calmodulin-like protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111499885 PE=4 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 1.5e-77
Identity = 157/194 (80.93%), Postives = 170/194 (87.63%), Query Frame = 0

Query: 1   MTNKGSSVLRLFTSCSRKRQPKNIPSSTQIKQRSNS---SSASSKHFRPNLEEIKWAFDK 60
           M NKG SVL LFTSCS KRQ K IPSSTQ KQR  S   SSASSKH + NLEEIKWAF+K
Sbjct: 1   MKNKGRSVLSLFTSCSPKRQLKQIPSSTQPKQRFKSISDSSASSKHVQVNLEEIKWAFEK 60

Query: 61  FDSNKDGKISFEEYKAAHRGLAGSKEISDAEAAKSFKFVDADGDGFVDLKEFVELYTMSG 120
           FD+NKDGKISFEEYKAAHR ++GS+EISD EAAKSFKFVDADGDGFV+LKEFVELYTMSG
Sbjct: 61  FDTNKDGKISFEEYKAAHRAMSGSQEISDVEAAKSFKFVDADGDGFVELKEFVELYTMSG 120

Query: 121 GEVRVGDIESAFKVYDSNGDGKISAEEVMEIMNTLGERTTVKACRQMVKGVDMDGDGFID 180
           GEV++GD++SAFKVYDSNGDG+ISAEEVMEIM  LGE TT+KACRQMVKGVD DGDGFID
Sbjct: 121 GEVKMGDVQSAFKVYDSNGDGRISAEEVMEIMKILGESTTLKACRQMVKGVDKDGDGFID 180

Query: 181 IHEFSKLMGMYSSK 192
           I EFS LMG YS K
Sbjct: 181 IDEFSSLMGTYSFK 194

BLAST of Lsi10G005700 vs. NCBI nr
Match: XP_038901754.1 (probable calcium-binding protein CML32 [Benincasa hispida])

HSP 1 Score: 347.8 bits (891), Expect = 6.0e-92
Identity = 176/194 (90.72%), Postives = 186/194 (95.88%), Query Frame = 0

Query: 1   MTNKGSSVLRLFTSCSRKRQPKNIPSSTQIKQRSNSSSASSKHFRPNLEEIKWAFDKFDS 60
           MTNKG SVL LFTSC+RKRQPKNIPSSTQIKQRSNSSS SSKHFRPNLEEIKWAF+KFDS
Sbjct: 1   MTNKGRSVLSLFTSCTRKRQPKNIPSSTQIKQRSNSSSTSSKHFRPNLEEIKWAFEKFDS 60

Query: 61  NKDGKISFEEYKAAHRGLAGSKEISDAEAAKSFKFVDADGDGFVDLKEFVELYTMSGGEV 120
           NKDGKISFEEY+AAHRG+AGSKEISD EAAKSFKFVD DGDGFVDLKEF+ELYTMSGGEV
Sbjct: 61  NKDGKISFEEYQAAHRGMAGSKEISDEEAAKSFKFVDVDGDGFVDLKEFLELYTMSGGEV 120

Query: 121 RVGDIESAFKVYDSNGDGKISAEEVMEIMNTLGERTTVKACRQMVKGVDMDGDGFIDIHE 180
           RVGDI+SAF+VYDSNGDGKISAEEVM IMNTLGERTT+KAC+QMVKGVD+DGDGFIDI E
Sbjct: 121 RVGDIKSAFQVYDSNGDGKISAEEVMAIMNTLGERTTLKACKQMVKGVDIDGDGFIDIDE 180

Query: 181 FSKLMGMYSSKFQN 195
           FSKLMG YSSKFQN
Sbjct: 181 FSKLMGTYSSKFQN 194

BLAST of Lsi10G005700 vs. NCBI nr
Match: XP_004148036.1 (probable calcium-binding protein CML32 [Cucumis sativus] >KGN62012.1 hypothetical protein Csa_006079 [Cucumis sativus])

HSP 1 Score: 309.3 bits (791), Expect = 2.3e-80
Identity = 159/189 (84.13%), Postives = 168/189 (88.89%), Query Frame = 0

Query: 6   SSVLRLFTSCSRKRQPKNIPSSTQIKQRSNSSSASSKHFRPNLEEIKWAFDKFDSNKDGK 65
           SS+L+LF SCS KR  KN   S  IK+RSNSSS SSKHFRPNLEEIKWAF+KFDSNKDGK
Sbjct: 2   SSLLKLFRSCSGKRPSKNTSFSIPIKERSNSSSPSSKHFRPNLEEIKWAFEKFDSNKDGK 61

Query: 66  ISFEEYKAAHRGLAGSKEISDAEAAKSFKFVDADGDGFVDLKEFVELYTMSGGEVRVGDI 125
           ISFEEYK AHRGLAGSKEI+DAEA KSFK VD DGDGFVDLKEFVELYTMS GEV+VGDI
Sbjct: 62  ISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDVDGDGFVDLKEFVELYTMSSGEVKVGDI 121

Query: 126 ESAFKVYDSNGDGKISAEEVMEIMNTLGERTTVKACRQMVKGVDMDGDGFIDIHEFSKLM 185
           ESAFKVYDSNGDGKISAEEVM IM  LGE TT+KAC+QMVKGVDMDGDGFID+ EFSKLM
Sbjct: 122 ESAFKVYDSNGDGKISAEEVMGIMKILGENTTLKACKQMVKGVDMDGDGFIDVQEFSKLM 181

Query: 186 GMYSSKFQN 195
           G YSSKFQN
Sbjct: 182 GKYSSKFQN 190

BLAST of Lsi10G005700 vs. NCBI nr
Match: XP_008457835.1 (PREDICTED: probable calcium-binding protein CML32 [Cucumis melo] >KAA0045818.1 putative calcium-binding protein CML32 [Cucumis melo var. makuwa] >TYJ99463.1 putative calcium-binding protein CML32 [Cucumis melo var. makuwa])

HSP 1 Score: 305.8 bits (782), Expect = 2.6e-79
Identity = 158/188 (84.04%), Postives = 167/188 (88.83%), Query Frame = 0

Query: 6   SSVLRLFTSCSRKRQPKNIPSSTQIKQRSNSSSASSKHFRPNLEEIKWAFDKFDSNKDGK 65
           +S+L+LF SCS KR  KN   S QIK+RSNSSS SSKHFRPNLEEIKWAF+KFDSNKDGK
Sbjct: 2   TSLLKLFRSCSGKRPSKNTSFSIQIKERSNSSSTSSKHFRPNLEEIKWAFEKFDSNKDGK 61

Query: 66  ISFEEYKAAHRGLAGSKEISDAEAAKSFKFVDADGDGFVDLKEFVELYTMSGGEVRVGDI 125
           ISFEEYK AHRGLAGSKEI+DAEA KSFK VDADGDGFVDLKEFVELYTMS GEV+VGDI
Sbjct: 62  ISFEEYKEAHRGLAGSKEITDAEAEKSFKLVDADGDGFVDLKEFVELYTMSSGEVKVGDI 121

Query: 126 ESAFKVYDSNGDGKISAEEVMEIMNTLGERTTVKACRQMVKGVDMDGDGFIDIHEFSKLM 185
           ESAFKVYDSNGDGKISAEEVM IM  LGE TT+ AC+QMVKGVDMDGDGFIDI EFSKLM
Sbjct: 122 ESAFKVYDSNGDGKISAEEVMGIMKILGENTTLNACKQMVKGVDMDGDGFIDIQEFSKLM 181

Query: 186 GMYSSKFQ 194
             YSSKFQ
Sbjct: 182 DTYSSKFQ 189

BLAST of Lsi10G005700 vs. NCBI nr
Match: KAG6605248.1 (Calmodulin-like protein 30, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 303.1 bits (775), Expect = 1.7e-78
Identity = 161/194 (82.99%), Postives = 170/194 (87.63%), Query Frame = 0

Query: 1   MTNKGSSVLRLFTSCSRKRQPKNIPSSTQIKQRSNS---SSASSKHFRPNLEEIKWAFDK 60
           M NKG SVL +FTSCS KRQ K IPSSTQ KQRS S   SSASSKH R NLEEIKWAFDK
Sbjct: 1   MKNKGRSVLSIFTSCSPKRQLKQIPSSTQPKQRSKSISDSSASSKHVRVNLEEIKWAFDK 60

Query: 61  FDSNKDGKISFEEYKAAHRGLAGSKEISDAEAAKSFKFVDADGDGFVDLKEFVELYTMSG 120
           FDSNKDGKISFEEYKAAH  ++GSKEISDAEAAKSFK VDADGDGFV+LKEFVELYTMSG
Sbjct: 61  FDSNKDGKISFEEYKAAHGAMSGSKEISDAEAAKSFKLVDADGDGFVELKEFVELYTMSG 120

Query: 121 GEVRVGDIESAFKVYDSNGDGKISAEEVMEIMNTLGERTTVKACRQMVKGVDMDGDGFID 180
           GEV++GDI+SAFKVYDSNGDGKISAEEVMEIM  LGE TT+KACRQMVKGVD DGDGFID
Sbjct: 121 GEVKMGDIQSAFKVYDSNGDGKISAEEVMEIMKILGESTTLKACRQMVKGVDKDGDGFID 180

Query: 181 IHEFSKLMGMYSSK 192
           + EFS LMG YS K
Sbjct: 181 MDEFSSLMGSYSFK 194

BLAST of Lsi10G005700 vs. NCBI nr
Match: XP_023532624.1 (calmodulin-like protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 302.8 bits (774), Expect = 2.2e-78
Identity = 160/194 (82.47%), Postives = 170/194 (87.63%), Query Frame = 0

Query: 1   MTNKGSSVLRLFTSCSRKRQPKNIPSSTQIKQRSNS---SSASSKHFRPNLEEIKWAFDK 60
           M NKG SVL LFTSCS KRQ K IPSSTQ KQRS S   SS SSKH R NLEEIKWAFDK
Sbjct: 1   MKNKGRSVLSLFTSCSPKRQLKQIPSSTQPKQRSKSISNSSTSSKHVRVNLEEIKWAFDK 60

Query: 61  FDSNKDGKISFEEYKAAHRGLAGSKEISDAEAAKSFKFVDADGDGFVDLKEFVELYTMSG 120
           FD+NKDGKISFEEYKAAH  ++GSKEISDAEAAKSF+FVDADGDGFV+LKEFVELYTMSG
Sbjct: 61  FDTNKDGKISFEEYKAAHGAMSGSKEISDAEAAKSFEFVDADGDGFVELKEFVELYTMSG 120

Query: 121 GEVRVGDIESAFKVYDSNGDGKISAEEVMEIMNTLGERTTVKACRQMVKGVDMDGDGFID 180
           GEV++GD++SAFKVYDSNGDGKISAEEVMEIM  LGE TT+KACRQMVKGVD DGDGFID
Sbjct: 121 GEVKMGDVQSAFKVYDSNGDGKISAEEVMEIMKILGESTTLKACRQMVKGVDKDGDGFID 180

Query: 181 IHEFSKLMGMYSSK 192
           I EFS LMG YS K
Sbjct: 181 IDEFSSLMGTYSFK 194

BLAST of Lsi10G005700 vs. TAIR 10
Match: AT2G15680.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 135.2 bits (339), Expect = 5.7e-32
Identity = 74/156 (47.44%), Postives = 102/156 (65.38%), Query Frame = 0

Query: 31  KQRSNSSSASSKHF-RPNLEEIKWAFDKFDSNKDGKISFEEYKAAHRGLAGSKEISDAEA 90
           +Q S  SS     F +P++ E++  F +FD +KDGKIS  EYK   R L   + I D   
Sbjct: 30  RQSSGLSSPGPGGFSQPSVNEMRRVFSRFDLDKDGKISQTEYKVVLRALGQERAIED--V 89

Query: 91  AKSFKFVDADGDGFVDLKEFVELYTMSGGEVRVGDIESAFKVYDSNGDGKISAEEVMEIM 150
            K FK VD DGDGF+D +EF++ Y  SGG +R  DI ++F  +D NGDGKISAEEVM ++
Sbjct: 90  PKIFKAVDLDGDGFIDFREFIDAYKRSGG-IRSSDIRNSFWTFDLNGDGKISAEEVMSVL 149

Query: 151 NTLGERTTVKACRQMVKGVDMDGDGFIDIHEFSKLM 186
             LGER +++ C +MV+ VD DGDG +++ EF K+M
Sbjct: 150 WKLGERCSLEDCNRMVRAVDADGDGLVNMEEFIKMM 182

BLAST of Lsi10G005700 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 114.4 bits (285), Expect = 1.0e-25
Identity = 62/144 (43.06%), Postives = 92/144 (63.89%), Query Frame = 0

Query: 47  NLEEIKWAFDKFDSNKDGKISFEEYKAAHRGLAGSKEISDAEAAKSFKFVDADGDGFVDL 106
           ++++IK  F +FD N DGKIS +E K   R L  S   S  E     K  D DG+GF+DL
Sbjct: 14  SMDDIKKVFQRFDKNGDGKISVDELKEVIRAL--SPTASPEETVTMMKQFDLDGNGFIDL 73

Query: 107 KEFVELYTM---SGGEVR--VGDIESAFKVYDSNGDGKISAEEVMEIMNTLGERTTVKAC 166
            EFV L+ +    GG  R  V D++ AF++YD +G+G+ISA+E+  +M  LGE+ +V+ C
Sbjct: 74  DEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDC 133

Query: 167 RQMVKGVDMDGDGFIDIHEFSKLM 186
           ++M+  VD+DGDG ++  EF K+M
Sbjct: 134 KKMISKVDIDGDGCVNFDEFKKMM 155

BLAST of Lsi10G005700 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 109.4 bits (272), Expect = 3.3e-24
Identity = 59/148 (39.86%), Postives = 88/148 (59.46%), Query Frame = 0

Query: 41  SKHFRPNLEEIKWAFDKFDSNKDGKISFEEYKAAHRGLAGSKEISDAEAAKSFKFVDADG 100
           S++   ++E+IK  F +FD N DGKIS +E K     L  S   S  E     K  D DG
Sbjct: 6   SRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGAL--SPNASQEETKAMMKEFDLDG 65

Query: 101 DGFVDLKEFVELYTM---SGGEVRVGDIESAFKVYDSNGDGKISAEEVMEIMNTLGERTT 160
           +GF+DL EFV L+ +   S     + D++ AF +YD + +G+ISA E+  +M  LGE+ +
Sbjct: 66  NGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCS 125

Query: 161 VKACRQMVKGVDMDGDGFIDIHEFSKLM 186
           ++ C++M+  VD DGDG +D  EF K+M
Sbjct: 126 IQDCQRMINKVDSDGDGCVDFEEFKKMM 151

BLAST of Lsi10G005700 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 105.1 bits (261), Expect = 6.3e-23
Identity = 67/177 (37.85%), Postives = 104/177 (58.76%), Query Frame = 0

Query: 18  KRQPKNIPSSTQIKQRSNSS-SASSKHFRPNLEEIKWAFDKFDSNKDGKISFEEYKAAHR 77
           K Q  N  SS+ +   ++S     ++  +  + E++  F KFD N DGKIS +E  A   
Sbjct: 4   KNQGSNGGSSSNVGIGADSPYLQKARSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMT 63

Query: 78  GLAGSKEISDAEAAKSFKFVDADGDGFVDLKEFVELYT--MSGGEVRVGDIESAFKVYDS 137
            L    E+ + E  K+   +D  GDG+++ +EFVEL T  M   +V + +++ AF VYD 
Sbjct: 64  SL--GHEVPEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDV-LENLKDAFSVYDI 123

Query: 138 NGDGKISAEEVMEIMNTLGERTTVKACRQMVKGVDMDGDGFIDIHEFSKLMGMYSSK 192
           +G+G ISAEE+ E++ +LG+  ++  CR+M+ GVD DGDG ID  EF  +M M S +
Sbjct: 124 DGNGSISAEELHEVLRSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMGSRR 177

BLAST of Lsi10G005700 vs. TAIR 10
Match: AT5G17470.1 (EF hand calcium-binding protein family )

HSP 1 Score: 104.4 bits (259), Expect = 1.1e-22
Identity = 59/143 (41.26%), Postives = 83/143 (58.04%), Query Frame = 0

Query: 55  FDKFDSNKDGKISFEEYKAAHRGLAGSKEISDAEAAKSFKFVDADGDGFVDLKEFVELYT 114
           F++ D NKDGKIS++E+  A R  A S  I+  E    F+ +D DGD  +D+ E+     
Sbjct: 7   FERVDKNKDGKISWDEFAEAIR--AFSPSITSEEIDNMFREIDVDGDNQIDVAEYASC-L 66

Query: 115 MSGGEVRVGD----IESAFKVYDSNGDGKISAEEVMEIMNTLGERTTVKACRQMVKGVDM 174
           M GGE    D    ++ AF +YD +GDGKISA E+  ++  LGE+ T+  C  MV+ VD 
Sbjct: 67  MLGGEGNKEDEDIVMKEAFDLYDIDGDGKISASEIHVVLKRLGEKQTIAECIAMVRAVDA 126

Query: 175 DGDGFIDIHEFSKLMGMYSSKFQ 194
           DGDGF+   EF  +M   + K Q
Sbjct: 127 DGDGFVSFEEFKTMMSCNNKKLQ 146