Lsi10G004570 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G004570
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionP-loop containing nucleoside triphosphate hydrolases superfamily protein
Locationchr10: 6637592 .. 6643330 (+)
RNA-Seq ExpressionLsi10G004570
SyntenyLsi10G004570
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGCAGAAAGGTAAAAATATAAAAGTTTATATAAAATGAATGGATTTACCTTTTTTTTTCAGGTCTTGATTTGAATTGATTTTGTTCAGAAAGTGGCAGCAAACCGAGTTTAGGGCAGATAGTTTGGGCTTCTTCGATTGGATTGATCATCGTTGCTGCTATGCATTATCGGATTCGAAAGCTAAGAGATCAAAAGATCATCCCTCGCTTGAAAGTTTCCGACACGGGTCGCGTTGAAAAGCTCGAGAGCTTTCCCCATTATGTAGGTAAAGATTATATACTTACGTTCTTTTCATAATTTACATTACTGAAATTGTTCCATTTCTTTAAGGAAATTTTTACACATGAAAAAAATGTCAAAATAGTAATATATATATATATATATTTTGATAGACATTGATAGACTTTTATCCGCATCTATCAGTGATAAACTAGACTTCTATCCGCATCTATCACTCATCTATCGCTGATAGACTTCTATCAGCATCTATAAGTGATAGACTAGATTTCTATCAGCATCTATCGCTCATCTATCACTTAGTGATAGACTTCTATCAGCATCTATCACTCAATAATAGATTTCTACATTTCTATCAGTGGTAGGCTCGATAGAAACGTCTATCACGATTCACAACTATCTAAAAAATTTTGTTATTTTGTGCAAATAGTTTCTTTATTTTTCTATTTTTAAAAAAATTCTCCATTTATTTAGCTTAGTTTTTCCTTTTTCTTTAGAATTTGTTTTTAAGAACCTCAAGCCTAAGTTTGTGAACTATGTTGGTAAATTATAGATAACTTAAATTACACATTTTGTCAGAAAATTAGAATTTAGTCCTAGAATTAGTCTCTATAAAGGTCTATTTATGAAATTTATCAAATTATAAATACCATTTATAAAGTTTTATCAAATCATAGGAACTAAATTTTAACTTTTAAAATAGTAAATTCTAACTTTCTCTCTACTATAAGAACCAAATTTGTAATTTGACCTTATAGATATTATTCTAATCTTATCCTCAATTTTTTAGTCAATCTGACCATGGCCCAACTAGTTAATTGGGGAGGAAATGGAATTCACAACTCTACTCTCAGTCCAAAACATCAATTTTATGTTTTATTAACTCTTTACATTGTGGATCCCACAACTTCACAACTCTTTTGACTTTATAACCCTCCAAACTTCACAACTCCATTCATTGTCCCAAACATCCCAAAGAGTATAGATAATTAGCCACTAACCATGAATTTGCATTTCTTTTTAGAAAATTATTTTTTAAGATGGGTAGGGGATGCTTGTTAAAAGTGTTTTTTGAGTGAAACTATTATTCTATAAGTACTTTTGACTAAAAACTAATTAAGTTTTTTTTTCTTTTTTAAAGTCGAAATGAGCTTAGTTCACTATCATAAAAGTATGTGTTAAGGACCAAGAGATTTATGATTCAAATCGTCCACCCTATTATACTAAAAATATATATATTTCCTCTTTTAAAAGTTAAAACAGTTTACCACTTTTGATTTCACAAATTAAAAAAAAATACATTTAACTAGCTTAAAACACTAATTAAAATTTTAACCAAACACAGCTCACTTTTATAAAATAATAATAATAATAAACCAACTTTTAAAAATCCATGAACAATTTGAAAGACATGTAAAAAAATTTAAGCCATTAAAAATGAAATTGTTATCAAGCAAGACTATCAGTTAGAGGATCGAGAAAATGATATCCACTTTTTTTTTGTTCAAATAAGACTGAAATAGAAATTAGAACAAAATTTTGAATCCAAAATAGTTGTTGCATTGACGTTCAGTCTCTAAACTTTTAAGTCTATGTTCATTTTATCTTTAAAATTTAAAAAGTGTTAATAGGCCTACGTCCAAAAGGTCCCTAAATTATCAAAAGTGTCTAATAACAAATCTTTATACTTTCAATTTTATGTCTATTAGGTGCATAAATTTTAAAAAATATTGGATAGGTTAATGACCTATTAAACACAAACTTTTTCCAATTTGACATTTTTTAAAATTCACGTGGATAATGTACACAAAACTAAAAGTTTATCCTCTTATTAGATATAAAATTCAATTTTTAATTTCACAAATCTATTATTATTTAAAAAATTTCAAACATGTCTAAGACTTGTAGACACAAATCGTAAAGTTGAGAGACCCATATAGGAATTCTTTAAAGTTGACCTACTAACCAACAAACTAAAACTCGGATGACCTAAAGTTTAAAGACTTTCTTTAAAAGTTCATATTCTAAACTTGTAACTTAACCCTAGTTGTTTTATTAAAAAAATTAAAACATAATATTAGAAATTTAATTCCAATTCCACAAATTATCATCTTGGTTGAATACCCATGTCTCATTCTCATTTCTTTTACATAAGCACCATCTAAAATTTCCAAACTCAATACCAATTTTGATACTTCTCTCTCTCAATATTTAACCTTTTATGTTAGATGATATAATATTAAATTTACCTTGACTCGTCAACTCAAACTTTTGGGTCAATTGGTAATTTAACATGGTGTCAACATAGGTGGTCCAAGAGATCCTGTATTTAAACTTAGTACTACGGTGGAATATTTTCCCTACTACCTTCATTTATTGTTTGCTACAAAAGTAGAATTTAAGAATTATATGGGGTCCACGAAATAAATGAATAATAAATTAATAACTCATTTGATGACGTTTCAAATAATGAATGGGAGTAGTAAGTACTATACTACTATAAATTTTTCCATTTCCTCCCCAATTAATATTGATGTTCACTCTCGAGTTCTCTACATATTTTAAGTCCACAAGTGAAAGGAAGTAAGATGATATAATATTAAATTTACCTTCATCTATCAACATAAGCTTTCAAGTCAATCGGTGACTTTAACATTCTATCGATAACTTGTCTACTTTTATTCTCAATTATCAGCAAGGCAAATGGGATTTAAAGATATACATGAATGCCCACATCTTTGCAAGTTGGCTTCTGAGTACATAGCTAAATCAGACGAATGTGAAGATGATATCTATTCCTTTTTCTCAAGTGAGCTGCATGCAGATTCACTTTTTGTCAAACTTGTGGAAGAGTTTGAGAGATGCATTCTCAGTTATTTTGCATTCCATTGGAATCAAACAGATATTATGATTAGTCAGGTGAAGTGTTTGATAAAATGTTTTTTAATTTTATCATAAATTGAGTTTCTTGTTTGTATAATTTGTGTATTGTTTGTATCTCTTTTTGTGTTTGTTCAGATTTTGAGTTCCGACCACCAGGAGCCAAAAAAGAAGCTCAGAAGCATTGTAATGGCTGCAACCAGGTGGGCATCTCTTGACCCTTCATTATTGTTGAAGTATCTAATATAATTAAATTTACCGTTACCCATCAACTTAGGCTTTGGGGTTGATTGGTGATTTAACACGGTATTAGAGCAGAAAATTTTGTATTCAAATCTTTGTAGTGTCATTGCTTTCTCAAATAATATTGATTTCCAATTTCGGGGGCTTCCATAAATTTTCAAGTCCCAAAAGTGAGGGGGGTGTTGAAATATTGATATAACTAAATTTACCCTAACTTACTAACTTAAACTTTTGGGTTGATTGGTGATTTAGTATGGTGTCAAAGCAAGAGATCCTATGTATGGACCTCATAAGATGTAATTTCCTATCCTATTAATATTCATTTTCCACTTGTTGAACTTTCTATAAATTTTCAAGTCCCACAAGTGAGAAAAAGTGTTGAAACATTGATATAATTGAATTTACCGTGTCCTATTATTTTTAAGCTTGTAGGTTGATTGGTGATTTAATTCTAAAAGTCAGAGACTAAATTTATAATTTAACTTTATATGAACTTATGGCCCCTCTACATGAATTTATGGATCTGGCACCGACTAGGATGTCACATTTTTTACATTAAATTCTTTATTAAAGAGATTATTGAGAAGAAAGCTATCGTATCAAGTCAAGTTTTTAAAAGTTGTGCTTTTGTTTTTTAAATTTGGCTAAGAACTCAAAGTTTTTAAGAAAGATGAAAATTGTTATCAAGAAATTATGAGAAAACAAGCACAATTTTTAAAGACGGAGTGATTATCAAACATACCTTAATCTTTTAAACTCATCTAAGAACAACCTTCACTTACATCCATATCATTTTTTGTGATATTGTTAGGGAACAGAGGTTTGAGAGGGTAACAAAGAACCTGAAAGTTGCAAGAGTATTTACAACTTTGGTTGAGGAGATGAAAGCAATGGGACTCGCATCCACCGACGACTCTCAATGCACAGAGGTGATGGCTCCAATGGCTCATGCCGATAGAAGTCCGATGCTCCTGTTCATGGGCGGCGGTATGGGGGCGGGGAAGAGTACTGTGCTTAAGGACATTCTGAAAGAGTAAGAATATTCATCTTGGGTTATGAAAATGAAGTCAAATTTCATCAGATATAGCTAAGTAAATTTGTACAATTATTAAGCATATATAATGGCATATATAATGTTAAACAGGCCATTCTGGGTAGGAGCAGGACCTAATGCAGTTGTAATTGAGGCAGATGCTTTTAAAGAATCAGATGTCATTTATAGAGCTCTTAACTCCACTGGTCACCATCATGACATGCTTCAAACTGCTGAACTGGTATCATTTCAGCTCTAAAATCTTCCATCAAAATCATTCCATGCTTTGAGATTATATATAAAATTTTCTCATTCTGCTCTTTGTGGCTTTCAGTTCATAAGATCATGGTTTTTACTTCTTTTATGACTTCGTGAAAACTAATTATCAGTAGGACCTGTTTGGAATGACTTTTCAAGTACTTAAAAACACTTCTTTTTTTTTTTGCTTAAAAACATTGTTAAGTAATTGAAAAGTCATTTCAAATCAGTTTATAGTCTCCAATACGTAACCAACATGCAGAATCATACACCCACCTAGTGCAGAGGCTGAGCATTTGCCTTACCACACCATGGTGTGTGGAGTTCGATCTTGCAACTATGCAGTTCGTTGTTTGTTTACGCAATAAGGTCATGGGTTTATTAAAGGACTTAGAGGGAATAGGTTTAATCCATAGTGGCCACATACCTAAGATTTAATATTCTATGAGTGTCCCAGACACCCAAATTTTGAGGGTCAAGTAGGTTGTCTCGTGAGATTAGTCGAGGTGTATGAAAGCTAACCCGAACACTCACGCATATAAAAAGAAAAAAGAAAACATACAGATGCTCCTTTTTCATTATTTTCCTAATGAAGGCAAAAGGCATTCTTATGAACCTCTTGTTTCTTTAGGCCGTGGGATTAAATCTTGAAATCTGTTATGAACCAGATATTCTTACACTTCACTTTTGATCCTTGCTGCACCATTTTACACCTTTTGATGTTTCAATTTAAAATATATGTTTCAAACAGGTGCACCAATCATCCACAGATGCAGCTTCATCACTTCTAGTGACAGCACTGAATGAAGGACGAGACGTAATCATGGATGGCACATTGTCGTGGGTACCGTTCGTAGTTCAGACGATTACGATGGCTAGGAATGTCCACCGTCGACGTTATCGAATGGGAGCTGGCTATAAGGTTGGTGATGATGGCACTGTAACCGAAAATTATTGGGAACGAATAGAAGATCAAGAACCAGATCAAGTTGGGGGCAAGAAAAGAAAGCCATACAGGATTGAACTTGTTGGAGTTGTTTGTGATGCTTATTTAGCTGTCATAAGAGGCATAAGGTACAGATAG

mRNA sequence

ATGATGCAGAAAGAAAGTGGCAGCAAACCGAGTTTAGGGCAGATAGTTTGGGCTTCTTCGATTGGATTGATCATCGTTGCTGCTATGCATTATCGGATTCGAAAGCTAAGAGATCAAAAGATCATCCCTCGCTTGAAAGTTTCCGACACGGGTCGCGTTGAAAAGCTCGAGAGCTTTCCCCATTATAGGTTTGAGAGGGTAACAAAGAACCTGAAAGTTGCAAGAGTATTTACAACTTTGGTTGAGGAGATGAAAGCAATGGGACTCGCATCCACCGACGACTCTCAATGCACAGAGGTGATGGCTCCAATGGCTCATGCCGATAGAAGTCCGATGCTCCTGTTCATGGGCGGCGGTATGGGGGCGGGGAAGAGTACTGTGCTTAAGGACATTCTGAAAGAGCCATTCTGGGTAGGAGCAGGACCTAATGCAGTTGTAATTGAGGCAGATGCTTTTAAAGAATCAGATGTCATTTATAGAGCTCTTAACTCCACTGGTCACCATCATGACATGCTTCAAACTGCTGAACTGGTGCACCAATCATCCACAGATGCAGCTTCATCACTTCTAGTGACAGCACTGAATGAAGGACGAGACGTAATCATGGATGGCACATTGTCGTGGGTACCGTTCGTAGTTCAGACGATTACGATGGCTAGGAATGTCCACCGTCGACGTTATCGAATGGGAGCTGGCTATAAGGTTGGTGATGATGGCACTGTAACCGAAAATTATTGGGAACGAATAGAAGATCAAGAACCAGATCAAGTTGGGGGCAAGAAAAGAAAGCCATACAGGATTGAACTTGTTGGAGTTGTTTGTGATGCTTATTTAGCTGTCATAAGAGGCATAAGGTACAGATAG

Coding sequence (CDS)

ATGATGCAGAAAGAAAGTGGCAGCAAACCGAGTTTAGGGCAGATAGTTTGGGCTTCTTCGATTGGATTGATCATCGTTGCTGCTATGCATTATCGGATTCGAAAGCTAAGAGATCAAAAGATCATCCCTCGCTTGAAAGTTTCCGACACGGGTCGCGTTGAAAAGCTCGAGAGCTTTCCCCATTATAGGTTTGAGAGGGTAACAAAGAACCTGAAAGTTGCAAGAGTATTTACAACTTTGGTTGAGGAGATGAAAGCAATGGGACTCGCATCCACCGACGACTCTCAATGCACAGAGGTGATGGCTCCAATGGCTCATGCCGATAGAAGTCCGATGCTCCTGTTCATGGGCGGCGGTATGGGGGCGGGGAAGAGTACTGTGCTTAAGGACATTCTGAAAGAGCCATTCTGGGTAGGAGCAGGACCTAATGCAGTTGTAATTGAGGCAGATGCTTTTAAAGAATCAGATGTCATTTATAGAGCTCTTAACTCCACTGGTCACCATCATGACATGCTTCAAACTGCTGAACTGGTGCACCAATCATCCACAGATGCAGCTTCATCACTTCTAGTGACAGCACTGAATGAAGGACGAGACGTAATCATGGATGGCACATTGTCGTGGGTACCGTTCGTAGTTCAGACGATTACGATGGCTAGGAATGTCCACCGTCGACGTTATCGAATGGGAGCTGGCTATAAGGTTGGTGATGATGGCACTGTAACCGAAAATTATTGGGAACGAATAGAAGATCAAGAACCAGATCAAGTTGGGGGCAAGAAAAGAAAGCCATACAGGATTGAACTTGTTGGAGTTGTTTGTGATGCTTATTTAGCTGTCATAAGAGGCATAAGGTACAGATAG

Protein sequence

MMQKESGSKPSLGQIVWASSIGLIIVAAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPHYRFERVTKNLKVARVFTTLVEEMKAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAGPNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVIMDGTLSWVPFVVQTITMARNVHRRRYRMGAGYKVGDDGTVTENYWERIEDQEPDQVGGKKRKPYRIELVGVVCDAYLAVIRGIRYR
Homology
BLAST of Lsi10G004570 vs. ExPASy Swiss-Prot
Match: Q0WUY1 (Calmodulin calcium-dependent NAD kinase OS=Arabidopsis thaliana OX=3702 GN=NADKC PE=1 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 9.8e-86
Identity = 174/269 (64.68%), Postives = 200/269 (74.35%), Query Frame = 0

Query: 17  WASSIGLIIVAAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPHYRFERVTKNLKVARV 76
           W  S  LI  A     +    DQK +P+LK        K       RFERVTK+LKV RV
Sbjct: 150 WTQSSNLISQA-----LSDESDQK-VPKLKDFVMAATRK------QRFERVTKDLKVKRV 209

Query: 77  FTTLVEEMKAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPF 136
            +TLVEEM+ +G  S+ +  CTEVM+P+AH  RSP+LL MGGGMGAGKSTVLKDI  E F
Sbjct: 210 ISTLVEEMRVIGSGSS-EPHCTEVMSPVAHNKRSPVLLLMGGGMGAGKSTVLKDIFLESF 269

Query: 137 WVGAGPNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNE 196
           W  A  +AVVIEADAFKE+DVIYRAL+S GHH DMLQTAELVHQSSTDAASSLLVTALN+
Sbjct: 270 WSEAQADAVVIEADAFKETDVIYRALSSRGHHDDMLQTAELVHQSSTDAASSLLVTALND 329

Query: 197 GRDVIMDGTLSWVPFVVQTITMARNVHRRRYRMGAGYKVGDDGTVTENYWERIEDQEPDQ 256
           GRDVIMDGTLSW PFV Q I MARNVH+++YRMG GYKV ++GT+TE YWE  E++  + 
Sbjct: 330 GRDVIMDGTLSWEPFVEQMIEMARNVHKQKYRMGEGYKVSEEGTITEKYWEEEEEETKEN 389

Query: 257 VGGKKRKPYRIELVGVVCDAYLAVIRGIR 286
              +  KPYRIELVGVVCDAYLAV RGIR
Sbjct: 390 GKQQNLKPYRIELVGVVCDAYLAVARGIR 405

BLAST of Lsi10G004570 vs. ExPASy TrEMBL
Match: A0A1S3C6U1 (uncharacterized protein LOC103497509 OS=Cucumis melo OX=3656 GN=LOC103497509 PE=4 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 2.6e-142
Identity = 279/381 (73.23%), Postives = 281/381 (73.75%), Query Frame = 0

Query: 5   ESGSKPSLGQIVWASSIGLIIVAAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPHY-- 64
           ESGSKPSLGQI+WASSIGLIIVAAMHYRIRKLRDQ+IIPRLKVSDTGRVEKLESFPHY  
Sbjct: 24  ESGSKPSLGQIIWASSIGLIIVAAMHYRIRKLRDQRIIPRLKVSDTGRVEKLESFPHYVA 83

Query: 65  ------------------------------------------------------------ 124
                                                                       
Sbjct: 84  RQMGFKDTHECPHLCKLASEYIAKSDECEDDIYSFFSSELHADSLFVKLVEEFERCILSY 143

Query: 125 --------------------------------------RFERVTKNLKVARVFTTLVEEM 184
                                                 RFERVTKNLKVARVFTTLVEEM
Sbjct: 144 FAFHWNQTDIMISQILSSDHQEPKKKLRSIVMAATREQRFERVTKNLKVARVFTTLVEEM 203

Query: 185 KAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAGPNA 244
           KAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAGPNA
Sbjct: 204 KAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAGPNA 263

Query: 245 VVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVIMDG 286
           VVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVIMDG
Sbjct: 264 VVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVIMDG 323

BLAST of Lsi10G004570 vs. ExPASy TrEMBL
Match: A0A0A0LJB0 (Zeta_toxin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G258840 PE=4 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 1.4e-140
Identity = 277/381 (72.70%), Postives = 279/381 (73.23%), Query Frame = 0

Query: 5   ESGSKPSLGQIVWASSIGLIIVAAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPHY-- 64
           ESGSKPSLGQIVWASSIGLIIVAAMHYRIRKLR Q+IIPRLKVSDTGRVEKLESFPHY  
Sbjct: 28  ESGSKPSLGQIVWASSIGLIIVAAMHYRIRKLRVQRIIPRLKVSDTGRVEKLESFPHYVA 87

Query: 65  ------------------------------------------------------------ 124
                                                                       
Sbjct: 88  RQMGFKDIHECPHLCKLASEYIAKSEECEDDIYSFFSSELHADSLFVKLVEEFERCILSY 147

Query: 125 --------------------------------------RFERVTKNLKVARVFTTLVEEM 184
                                                 RFERVTKNLKVARVFTTLVEEM
Sbjct: 148 FAFHWNQTDIMISQILSSDHQEPKKKLRSIVMAATREQRFERVTKNLKVARVFTTLVEEM 207

Query: 185 KAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAGPNA 244
           KAMGLASTDDSQCTEVM PMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAGPNA
Sbjct: 208 KAMGLASTDDSQCTEVMVPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAGPNA 267

Query: 245 VVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVIMDG 286
           VVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVIMDG
Sbjct: 268 VVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVIMDG 327

BLAST of Lsi10G004570 vs. ExPASy TrEMBL
Match: A0A6J1G6X9 (uncharacterized protein LOC111451387 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111451387 PE=4 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 5.1e-138
Identity = 271/384 (70.57%), Postives = 277/384 (72.14%), Query Frame = 0

Query: 2   MQKESGSKPSLGQIVWASSIGLIIVAAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPH 61
           MQK+SGSKPS  QIVWASSIGLII+AAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPH
Sbjct: 1   MQKDSGSKPSFAQIVWASSIGLIIIAAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPH 60

Query: 62  Y----------------------------------------------------------- 121
           Y                                                           
Sbjct: 61  YVARQMGFKDINECPHLCKLASEYIAKSDECEDDIYSFFSSELHADSLFVKLVEEFERCI 120

Query: 122 -----------------------------------------RFERVTKNLKVARVFTTLV 181
                                                    RF+RVTKNLKVARVFTTLV
Sbjct: 121 LSYFAFHWNQTDIMISQILSSDHQEPKKKLRNIVMAATREQRFQRVTKNLKVARVFTTLV 180

Query: 182 EEMKAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAG 241
           EEMKAMGLASTDDSQCTEVM PMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAG
Sbjct: 181 EEMKAMGLASTDDSQCTEVMVPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAG 240

Query: 242 PNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVI 286
           PNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVI
Sbjct: 241 PNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVI 300

BLAST of Lsi10G004570 vs. ExPASy TrEMBL
Match: A0A6J1KZI6 (uncharacterized protein LOC111499679 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499679 PE=4 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 6.7e-138
Identity = 270/384 (70.31%), Postives = 277/384 (72.14%), Query Frame = 0

Query: 2   MQKESGSKPSLGQIVWASSIGLIIVAAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPH 61
           MQK+SGSKPS  QIVWASSIGLII+AAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPH
Sbjct: 1   MQKDSGSKPSFAQIVWASSIGLIIIAAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPH 60

Query: 62  Y----------------------------------------------------------- 121
           Y                                                           
Sbjct: 61  YVARQMGFKDINECPHLCKLASEYIAKSDECEDDIYSFFSSELHADSLFVKLVEEFERCI 120

Query: 122 -----------------------------------------RFERVTKNLKVARVFTTLV 181
                                                    RF+RVTKNLKVARVFTTLV
Sbjct: 121 LSYFAFHWNQTDIMISQILSSDHQEPKKKLRNIVMAATREQRFQRVTKNLKVARVFTTLV 180

Query: 182 EEMKAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAG 241
           EEMKAMGLASTDDSQCTEVM PMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAG
Sbjct: 181 EEMKAMGLASTDDSQCTEVMVPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAG 240

Query: 242 PNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVI 286
           PNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVI
Sbjct: 241 PNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVI 300

BLAST of Lsi10G004570 vs. ExPASy TrEMBL
Match: A0A6J1KXL4 (uncharacterized protein LOC111499679 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499679 PE=4 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 6.7e-138
Identity = 270/384 (70.31%), Postives = 277/384 (72.14%), Query Frame = 0

Query: 2   MQKESGSKPSLGQIVWASSIGLIIVAAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPH 61
           MQK+SGSKPS  QIVWASSIGLII+AAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPH
Sbjct: 1   MQKDSGSKPSFAQIVWASSIGLIIIAAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPH 60

Query: 62  Y----------------------------------------------------------- 121
           Y                                                           
Sbjct: 61  YVARQMGFKDINECPHLCKLASEYIAKSDECEDDIYSFFSSELHADSLFVKLVEEFERCI 120

Query: 122 -----------------------------------------RFERVTKNLKVARVFTTLV 181
                                                    RF+RVTKNLKVARVFTTLV
Sbjct: 121 LSYFAFHWNQTDIMISQILSSDHQEPKKKLRNIVMAATREQRFQRVTKNLKVARVFTTLV 180

Query: 182 EEMKAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAG 241
           EEMKAMGLASTDDSQCTEVM PMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAG
Sbjct: 181 EEMKAMGLASTDDSQCTEVMVPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAG 240

Query: 242 PNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVI 286
           PNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVI
Sbjct: 241 PNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVI 300

BLAST of Lsi10G004570 vs. NCBI nr
Match: XP_038901153.1 (uncharacterized protein LOC120088136 isoform X1 [Benincasa hispida])

HSP 1 Score: 519.2 bits (1336), Expect = 2.2e-143
Identity = 281/385 (72.99%), Postives = 285/385 (74.03%), Query Frame = 0

Query: 1   MMQKESGSKPSLGQIVWASSIGLIIVAAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFP 60
           MMQKESGSKPSLGQIVWASSIGLIIVAAMHYRIRK+RDQ+IIPRLKVSDTGRV+KLESFP
Sbjct: 1   MMQKESGSKPSLGQIVWASSIGLIIVAAMHYRIRKIRDQRIIPRLKVSDTGRVKKLESFP 60

Query: 61  HY---------------------------------------------------------- 120
           HY                                                          
Sbjct: 61  HYVARQMGFKDTHECPHLCKLASEYIAKSEECEDDIYSFFSSELHADSLFVKLVEEFERC 120

Query: 121 ------------------------------------------RFERVTKNLKVARVFTTL 180
                                                     RFERVTKNLKVARVFTTL
Sbjct: 121 ILSYFAFHWNQTDIMISQILSSDHQEPKKKLRSIVMAATREQRFERVTKNLKVARVFTTL 180

Query: 181 VEEMKAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGA 240
           VEEMKAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGA
Sbjct: 181 VEEMKAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGA 240

Query: 241 GPNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDV 286
           GPNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDV
Sbjct: 241 GPNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDV 300

BLAST of Lsi10G004570 vs. NCBI nr
Match: XP_008457945.1 (PREDICTED: uncharacterized protein LOC103497509 [Cucumis melo])

HSP 1 Score: 514.6 bits (1324), Expect = 5.4e-142
Identity = 279/381 (73.23%), Postives = 281/381 (73.75%), Query Frame = 0

Query: 5   ESGSKPSLGQIVWASSIGLIIVAAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPHY-- 64
           ESGSKPSLGQI+WASSIGLIIVAAMHYRIRKLRDQ+IIPRLKVSDTGRVEKLESFPHY  
Sbjct: 24  ESGSKPSLGQIIWASSIGLIIVAAMHYRIRKLRDQRIIPRLKVSDTGRVEKLESFPHYVA 83

Query: 65  ------------------------------------------------------------ 124
                                                                       
Sbjct: 84  RQMGFKDTHECPHLCKLASEYIAKSDECEDDIYSFFSSELHADSLFVKLVEEFERCILSY 143

Query: 125 --------------------------------------RFERVTKNLKVARVFTTLVEEM 184
                                                 RFERVTKNLKVARVFTTLVEEM
Sbjct: 144 FAFHWNQTDIMISQILSSDHQEPKKKLRSIVMAATREQRFERVTKNLKVARVFTTLVEEM 203

Query: 185 KAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAGPNA 244
           KAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAGPNA
Sbjct: 204 KAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAGPNA 263

Query: 245 VVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVIMDG 286
           VVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVIMDG
Sbjct: 264 VVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVIMDG 323

BLAST of Lsi10G004570 vs. NCBI nr
Match: XP_011650155.2 (uncharacterized protein LOC101203899 [Cucumis sativus])

HSP 1 Score: 508.8 bits (1309), Expect = 3.0e-140
Identity = 277/381 (72.70%), Postives = 279/381 (73.23%), Query Frame = 0

Query: 5   ESGSKPSLGQIVWASSIGLIIVAAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPHY-- 64
           ESGSKPSLGQIVWASSIGLIIVAAMHYRIRKLR Q+IIPRLKVSDTGRVEKLESFPHY  
Sbjct: 39  ESGSKPSLGQIVWASSIGLIIVAAMHYRIRKLRVQRIIPRLKVSDTGRVEKLESFPHYVA 98

Query: 65  ------------------------------------------------------------ 124
                                                                       
Sbjct: 99  RQMGFKDIHECPHLCKLASEYIAKSEECEDDIYSFFSSELHADSLFVKLVEEFERCILSY 158

Query: 125 --------------------------------------RFERVTKNLKVARVFTTLVEEM 184
                                                 RFERVTKNLKVARVFTTLVEEM
Sbjct: 159 FAFHWNQTDIMISQILSSDHQEPKKKLRSIVMAATREQRFERVTKNLKVARVFTTLVEEM 218

Query: 185 KAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAGPNA 244
           KAMGLASTDDSQCTEVM PMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAGPNA
Sbjct: 219 KAMGLASTDDSQCTEVMVPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAGPNA 278

Query: 245 VVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVIMDG 286
           VVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVIMDG
Sbjct: 279 VVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVIMDG 338

BLAST of Lsi10G004570 vs. NCBI nr
Match: XP_022947558.1 (uncharacterized protein LOC111451387 isoform X2 [Cucurbita moschata])

HSP 1 Score: 500.4 bits (1287), Expect = 1.1e-137
Identity = 271/384 (70.57%), Postives = 277/384 (72.14%), Query Frame = 0

Query: 2   MQKESGSKPSLGQIVWASSIGLIIVAAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPH 61
           MQK+SGSKPS  QIVWASSIGLII+AAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPH
Sbjct: 1   MQKDSGSKPSFAQIVWASSIGLIIIAAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPH 60

Query: 62  Y----------------------------------------------------------- 121
           Y                                                           
Sbjct: 61  YVARQMGFKDINECPHLCKLASEYIAKSDECEDDIYSFFSSELHADSLFVKLVEEFERCI 120

Query: 122 -----------------------------------------RFERVTKNLKVARVFTTLV 181
                                                    RF+RVTKNLKVARVFTTLV
Sbjct: 121 LSYFAFHWNQTDIMISQILSSDHQEPKKKLRNIVMAATREQRFQRVTKNLKVARVFTTLV 180

Query: 182 EEMKAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAG 241
           EEMKAMGLASTDDSQCTEVM PMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAG
Sbjct: 181 EEMKAMGLASTDDSQCTEVMVPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAG 240

Query: 242 PNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVI 286
           PNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVI
Sbjct: 241 PNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVI 300

BLAST of Lsi10G004570 vs. NCBI nr
Match: XP_023533279.1 (uncharacterized protein LOC111795219 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 500.4 bits (1287), Expect = 1.1e-137
Identity = 271/384 (70.57%), Postives = 277/384 (72.14%), Query Frame = 0

Query: 2   MQKESGSKPSLGQIVWASSIGLIIVAAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPH 61
           MQK+SGSKPS  QIVWASSIGLII+AAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPH
Sbjct: 1   MQKDSGSKPSFAQIVWASSIGLIIIAAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPH 60

Query: 62  Y----------------------------------------------------------- 121
           Y                                                           
Sbjct: 61  YVARQMGFKDINECPHLCKLASEYIAKSDECEDDIYSFFSSELHADSLFVKLVEEFERCI 120

Query: 122 -----------------------------------------RFERVTKNLKVARVFTTLV 181
                                                    RF+RVTKNLKVARVFTTLV
Sbjct: 121 LSYFAFHWNQTDIMISQILSSDHQEPKKKLRNIVMAATREQRFQRVTKNLKVARVFTTLV 180

Query: 182 EEMKAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAG 241
           EEMKAMGLASTDDSQCTEVM PMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAG
Sbjct: 181 EEMKAMGLASTDDSQCTEVMVPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAG 240

Query: 242 PNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVI 286
           PNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVI
Sbjct: 241 PNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVI 300

BLAST of Lsi10G004570 vs. TAIR 10
Match: AT1G06750.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 384.0 bits (985), Expect = 1.0e-106
Identity = 225/386 (58.29%), Postives = 249/386 (64.51%), Query Frame = 0

Query: 1   MMQKESGSKPS-LGQIVWASSIGLIIVAAMHYRIRKLRDQKIIPRLKVSDTGR-VEKLES 60
           M ++ SG++ S   QI+  SSIGL+I AA+HYR+R+LRD KIIPRLK S   +  EKLE 
Sbjct: 1   MQREYSGNRTSTYSQILVVSSIGLMIAAAVHYRLRRLRDTKIIPRLKSSHKHKGHEKLER 60

Query: 61  FPHY-------------------------------------------------------- 120
           F HY                                                        
Sbjct: 61  FSHYVARQMGFKDRRECPNLCKLAAEYISKSECCEEDIYSFFSEEPDADTLFIKLVEEFE 120

Query: 121 -------------------------------------------RFERVTKNLKVARVFTT 180
                                                      R +RVTKNLKVARVF T
Sbjct: 121 RCILSYFAFHWSHADLMITQVLSADAEPKRKLKQIVMAATRELRIKRVTKNLKVARVFNT 180

Query: 181 LVEEMKAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVG 240
           LVEEM+AMGLAS DDSQCTEVMAP+AH DRSP+LL MGGGMGAGKSTVLKDILKE FW G
Sbjct: 181 LVEEMRAMGLASVDDSQCTEVMAPVAHKDRSPVLLLMGGGMGAGKSTVLKDILKEAFWAG 240

Query: 241 AGPNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRD 286
           A  ++VVIEADAFKESDVIYRAL+S G H DM+QTAELVHQSSTDAASSLLVTALNEGRD
Sbjct: 241 A--DSVVIEADAFKESDVIYRALSSRG-HVDMIQTAELVHQSSTDAASSLLVTALNEGRD 300

BLAST of Lsi10G004570 vs. TAIR 10
Match: AT1G06750.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 382.1 bits (980), Expect = 3.9e-106
Identity = 224/381 (58.79%), Postives = 246/381 (64.57%), Query Frame = 0

Query: 6   SGSKPS-LGQIVWASSIGLIIVAAMHYRIRKLRDQKIIPRLKVSDTGR-VEKLESFPHY- 65
           SG++ S   QI+  SSIGL+I AA+HYR+R+LRD KIIPRLK S   +  EKLE F HY 
Sbjct: 13  SGNRTSTYSQILVVSSIGLMIAAAVHYRLRRLRDTKIIPRLKSSHKHKGHEKLERFSHYV 72

Query: 66  ------------------------------------------------------------ 125
                                                                       
Sbjct: 73  ARQMGFKDRRECPNLCKLAAEYISKSECCEEDIYSFFSEEPDADTLFIKLVEEFERCILS 132

Query: 126 --------------------------------------RFERVTKNLKVARVFTTLVEEM 185
                                                 R +RVTKNLKVARVF TLVEEM
Sbjct: 133 YFAFHWSHADLMITQVLSADAEPKRKLKQIVMAATRELRIKRVTKNLKVARVFNTLVEEM 192

Query: 186 KAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAGPNA 245
           +AMGLAS DDSQCTEVMAP+AH DRSP+LL MGGGMGAGKSTVLKDILKE FW GA  ++
Sbjct: 193 RAMGLASVDDSQCTEVMAPVAHKDRSPVLLLMGGGMGAGKSTVLKDILKEAFWAGA--DS 252

Query: 246 VVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVIMDG 286
           VVIEADAFKESDVIYRAL+S G H DM+QTAELVHQSSTDAASSLLVTALNEGRDVIMDG
Sbjct: 253 VVIEADAFKESDVIYRALSSRG-HVDMIQTAELVHQSSTDAASSLLVTALNEGRDVIMDG 312

BLAST of Lsi10G004570 vs. TAIR 10
Match: AT2G30630.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 370.9 bits (951), Expect = 9.1e-103
Identity = 215/375 (57.33%), Postives = 240/375 (64.00%), Query Frame = 0

Query: 11  SLGQIVWASSIGLIIVAAMHYRIRKLRDQKIIPRLKVSDTGR-VEKLESFPHY------- 70
           +  QI+  SSIGL++  A+H+R+RKLR  K IPRL+ S   +  EKLE F HY       
Sbjct: 26  TFAQILVISSIGLLLALALHFRLRKLRHSKNIPRLRSSQKHKGHEKLERFSHYVVRQMGF 85

Query: 71  ------------------------------------------------------------ 130
                                                                       
Sbjct: 86  KDRRECPHLCKLANEYIRKSGSCEEDIYSFFSEEPGADSLFIKLVEEFERCILSYFAYHW 145

Query: 131 --------------------------------RFERVTKNLKVARVFTTLVEEMKAMGLA 190
                                           RFERVTKNLKVARVF TLVEEMKAMG+A
Sbjct: 146 SHADLMISQILSADVEPKKKLKHIVMAATREQRFERVTKNLKVARVFNTLVEEMKAMGIA 205

Query: 191 STDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPFWVGAGPNAVVIEAD 250
           S DDS+CTEVMAP+AH DRSP+LL MGGGMGAGKSTVLKDILKEPFW GA  +AVVIEAD
Sbjct: 206 SVDDSECTEVMAPVAHKDRSPVLLLMGGGMGAGKSTVLKDILKEPFWAGA--DAVVIEAD 265

Query: 251 AFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNEGRDVIMDGTLSWVP 286
           AFKESDVIYRAL++ G H DM++TAE VHQSSTDAASSLLVTALNEGRDVIMDGTLSWVP
Sbjct: 266 AFKESDVIYRALSARG-HVDMIKTAEFVHQSSTDAASSLLVTALNEGRDVIMDGTLSWVP 325

BLAST of Lsi10G004570 vs. TAIR 10
Match: AT2G30630.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 366.7 bits (940), Expect = 1.7e-101
Identity = 188/223 (84.30%), Postives = 204/223 (91.48%), Query Frame = 0

Query: 63  RFERVTKNLKVARVFTTLVEEMKAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGA 122
           RFERVTKNLKVARVF TLVEEMKAMG+AS DDS+CTEVMAP+AH DRSP+LL MGGGMGA
Sbjct: 199 RFERVTKNLKVARVFNTLVEEMKAMGIASVDDSECTEVMAPVAHKDRSPVLLLMGGGMGA 258

Query: 123 GKSTVLKDILKEPFWVGAGPNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSS 182
           GKSTVLKDILKEPFW GA  +AVVIEADAFKESDVIYRAL++ G H DM++TAE VHQSS
Sbjct: 259 GKSTVLKDILKEPFWAGA--DAVVIEADAFKESDVIYRALSARG-HVDMIKTAEFVHQSS 318

Query: 183 TDAASSLLVTALNEGRDVIMDGTLSWVPFVVQTITMARNVHRRRYRMGAGYKVGDDGTVT 242
           TDAASSLLVTALNEGRDVIMDGTLSWVPFVVQTITMARNVHR RYRMGAGYKVG++G V 
Sbjct: 319 TDAASSLLVTALNEGRDVIMDGTLSWVPFVVQTITMARNVHRHRYRMGAGYKVGENGDVI 378

Query: 243 ENYWERIEDQEPDQVGGKKRKPYRIELVGVVCDAYLAVIRGIR 286
           ENYWERI +++  Q  G++RKPYRIELVGVVCDAYLAVIRGIR
Sbjct: 379 ENYWERIGERQQLQEDGRERKPYRIELVGVVCDAYLAVIRGIR 418


HSP 2 Score: 51.6 bits (122), Expect = 1.2e-06
Identity = 27/53 (50.94%), Postives = 36/53 (67.92%), Query Frame = 0

Query: 11 SLGQIVWASSIGLIIVAAMHYRIRKLRDQKIIPRLKVSDTGR-VEKLESFPHY 63
          +  QI+  SSIGL++  A+H+R+RKLR  K IPRL+ S   +  EKLE F HY
Sbjct: 26 TFAQILVISSIGLLLALALHFRLRKLRHSKNIPRLRSSQKHKGHEKLERFSHY 78

BLAST of Lsi10G004570 vs. TAIR 10
Match: AT1G04280.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 318.2 bits (814), Expect = 7.0e-87
Identity = 174/269 (64.68%), Postives = 200/269 (74.35%), Query Frame = 0

Query: 17  WASSIGLIIVAAMHYRIRKLRDQKIIPRLKVSDTGRVEKLESFPHYRFERVTKNLKVARV 76
           W  S  LI  A     +    DQK +P+LK        K       RFERVTK+LKV RV
Sbjct: 150 WTQSSNLISQA-----LSDESDQK-VPKLKDFVMAATRK------QRFERVTKDLKVKRV 209

Query: 77  FTTLVEEMKAMGLASTDDSQCTEVMAPMAHADRSPMLLFMGGGMGAGKSTVLKDILKEPF 136
            +TLVEEM+ +G  S+ +  CTEVM+P+AH  RSP+LL MGGGMGAGKSTVLKDI  E F
Sbjct: 210 ISTLVEEMRVIGSGSS-EPHCTEVMSPVAHNKRSPVLLLMGGGMGAGKSTVLKDIFLESF 269

Query: 137 WVGAGPNAVVIEADAFKESDVIYRALNSTGHHHDMLQTAELVHQSSTDAASSLLVTALNE 196
           W  A  +AVVIEADAFKE+DVIYRAL+S GHH DMLQTAELVHQSSTDAASSLLVTALN+
Sbjct: 270 WSEAQADAVVIEADAFKETDVIYRALSSRGHHDDMLQTAELVHQSSTDAASSLLVTALND 329

Query: 197 GRDVIMDGTLSWVPFVVQTITMARNVHRRRYRMGAGYKVGDDGTVTENYWERIEDQEPDQ 256
           GRDVIMDGTLSW PFV Q I MARNVH+++YRMG GYKV ++GT+TE YWE  E++  + 
Sbjct: 330 GRDVIMDGTLSWEPFVEQMIEMARNVHKQKYRMGEGYKVSEEGTITEKYWEEEEEETKEN 389

Query: 257 VGGKKRKPYRIELVGVVCDAYLAVIRGIR 286
              +  KPYRIELVGVVCDAYLAV RGIR
Sbjct: 390 GKQQNLKPYRIELVGVVCDAYLAVARGIR 405

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0WUY19.8e-8664.68Calmodulin calcium-dependent NAD kinase OS=Arabidopsis thaliana OX=3702 GN=NADKC... [more]
Match NameE-valueIdentityDescription
A0A1S3C6U12.6e-14273.23uncharacterized protein LOC103497509 OS=Cucumis melo OX=3656 GN=LOC103497509 PE=... [more]
A0A0A0LJB01.4e-14072.70Zeta_toxin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G258840 ... [more]
A0A6J1G6X95.1e-13870.57uncharacterized protein LOC111451387 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1KZI66.7e-13870.31uncharacterized protein LOC111499679 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1KXL46.7e-13870.31uncharacterized protein LOC111499679 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
XP_038901153.12.2e-14372.99uncharacterized protein LOC120088136 isoform X1 [Benincasa hispida][more]
XP_008457945.15.4e-14273.23PREDICTED: uncharacterized protein LOC103497509 [Cucumis melo][more]
XP_011650155.23.0e-14072.70uncharacterized protein LOC101203899 [Cucumis sativus][more]
XP_022947558.11.1e-13770.57uncharacterized protein LOC111451387 isoform X2 [Cucurbita moschata][more]
XP_023533279.11.1e-13770.57uncharacterized protein LOC111795219 isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G06750.11.0e-10658.29P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G06750.23.9e-10658.79P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G30630.19.1e-10357.33P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G30630.21.7e-10184.30P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT1G04280.17.0e-8764.68P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010488Zeta toxin domainPFAMPF06414Zeta_toxincoord: 106..221
e-value: 1.1E-14
score: 54.2
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 94..287
e-value: 1.1E-26
score: 95.6
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 109..243
IPR044802Calmodulin calcium-dependent NAD kinase NADKc-likePANTHERPTHR31153UNCHARACTERIZEDcoord: 3..62
coord: 63..285
NoneNo IPR availablePANTHERPTHR31153:SF13P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASES SUPERFAMILY PROTEINcoord: 3..62
coord: 63..285

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G004570.1Lsi10G004570.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0006357 regulation of transcription by RNA polymerase II
cellular_component GO:0016020 membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0016301 kinase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding