Lsi10G004300 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G004300
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionPentatricopeptide repeat-containing protein
Locationchr10: 6534116 .. 6535546 (-)
RNA-Seq ExpressionLsi10G004300
SyntenyLsi10G004300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGAGCCCTCACCATTTTCACATTAGACGAACTCTTGATTCCTTCGCCAGTTTCTTCCGCCATTCTTCTCAAATCAAAAATCTTAAAGCCATTAAGAAGATTCACGCCCATTTTCTTAGAACCCACTTCCAATTTGTCTCCCCCAATCTTCACTCTAAGCTTATACTATCCTACTCCCAAATCATCCCCAATTTTGATGCGCAAAGCTTAAACAGCATCTTCAAATGCATCATCCCCAGAAGTTCTTTAACCTTCAACGTAATAATTTCTGACTTTTCTCGACATGGGTTTCCAGATTTTGCGTTAATGGCGTTTTCTTTCATGCATTCTAATGGGGTTCCTATTGACACGTATGCCCTCTGCAGCTCTTTGAGTGCTTGTTCTCTTATTCAGAATGCGGTGTATGGAAAACAGATTCACGCATCCGTGGGGAAATCAGGATGGTTATGTAGTGTTTTTGTGGGGAGTGCGATTGTTGATATGTATGCAAAAAATTCGTTGATTAATGATGCAGTTGAAGTGTTTGATGAAATTCCCATGAAGAATACTGTGTGCGCAAATGCATTGTTGGCTGGCTACGGCGAGGCTAGGATGTGGGTTGAGGGACTTGAATTGCTACGGAAGATGCAGGCTCTGGATTTGAAGTACGACAAGTTCACTCTGTCTGCTTCCTTACGTGCATGCGCTGGTTTATCTGCAATTGCACTAGGTAAGCAAGTGCATGCTTATCTACTGCGAACGGTTTATGATATTACAAGTGATGTTCATCTGCAAAGCACATTAATTGAAATGTATGGGAAGAGTGGCATGGTGGAAAAAGCATGGAATGTCTTCGATTTCGCAGGACTTGGACAAAAAGAAGATGGGAAAAGGGATATTATAATGTGGACTTCTATGCTTAGTGTATATGGTAGAAACGGGTGTTACAAAAGAGTAATTGAACTGTACAACCAGATGTTGATGGAAGGGAACAAACCAGATAGGGTGGCTTTTGTGACAGTCATATCAGCTTGTGGTCATACTGGTGAGGTGAATCTTGGAATCAAGTATTTTGACTCCATGCAGAGTGACTTTGGGTTGGAACCTGGTCAAGAGCACTACAGTTGTCTGGTTGACTTGCTCTGTAGAGCTGGGGAATTGGAAAAAGCATGGAAGCTCGTAAATGATATCAAAAGTCGGGATTACAATATTTCCCTTTGGGGAGCTTTACTCCGTGCTTGCTTGGAACAGGGAAATATTAAGTTGGGTAATTTGGCAGCTCGTAGGGCACTTGAATTGGATCCTCAGAATGCTGGAATATTTGTTATGCTATCAAATCTGTATGCTGGTTTTGGCCTGTGGAATGAGATAGAGCATTTGAGGGAATTCGTGAGAAAGGAAGGGCTGTATAAAGGTGTTGGATGTAGCTGGATAGAGATCACAAGTTGA

mRNA sequence

ATGTCGAGCCCTCACCATTTTCACATTAGACGAACTCTTGATTCCTTCGCCAGTTTCTTCCGCCATTCTTCTCAAATCAAAAATCTTAAAGCCATTAAGAAGATTCACGCCCATTTTCTTAGAACCCACTTCCAATTTGTCTCCCCCAATCTTCACTCTAAGCTTATACTATCCTACTCCCAAATCATCCCCAATTTTGATGCGCAAAGCTTAAACAGCATCTTCAAATGCATCATCCCCAGAAGTTCTTTAACCTTCAACGTAATAATTTCTGACTTTTCTCGACATGGGTTTCCAGATTTTGCGTTAATGGCGTTTTCTTTCATGCATTCTAATGGGGTTCCTATTGACACGTATGCCCTCTGCAGCTCTTTGAGTGCTTGTTCTCTTATTCAGAATGCGGTGTATGGAAAACAGATTCACGCATCCGTGGGGAAATCAGGATGGTTATGTAGTGTTTTTGTGGGGAGTGCGATTGTTGATATGTATGCAAAAAATTCGTTGATTAATGATGCAGTTGAAGTGTTTGATGAAATTCCCATGAAGAATACTGTGTGCGCAAATGCATTGTTGGCTGGCTACGGCGAGGCTAGGATGTGGGTTGAGGGACTTGAATTGCTACGGAAGATGCAGGCTCTGGATTTGAAGTACGACAAGTTCACTCTGTCTGCTTCCTTACGTGCATGCGCTGGTTTATCTGCAATTGCACTAGGTAAGCAAGTGCATGCTTATCTACTGCGAACGGTTTATGATATTACAAGTGATGTTCATCTGCAAAGCACATTAATTGAAATGTATGGGAAGAGTGGCATGGTGGAAAAAGCATGGAATGTCTTCGATTTCGCAGGACTTGGACAAAAAGAAGATGGGAAAAGGGATATTATAATGTGGACTTCTATGCTTAGTGTATATGGTAGAAACGGGTGTTACAAAAGAGTAATTGAACTGTACAACCAGATGTTGATGGAAGGGAACAAACCAGATAGGGTGGCTTTTGTGACAGTCATATCAGCTTGTGGTCATACTGGTGAGGTGAATCTTGGAATCAAGTATTTTGACTCCATGCAGAGTGACTTTGGGTTGGAACCTGGTCAAGAGCACTACAGTTGTCTGGTTGACTTGCTCTGTAGAGCTGGGGAATTGGAAAAAGCATGGAAGCTCGTAAATGATATCAAAAGTCGGGATTACAATATTTCCCTTTGGGGAGCTTTACTCCGTGCTTGCTTGGAACAGGGAAATATTAAGTTGGGTAATTTGGCAGCTCGTAGGGCACTTGAATTGGATCCTCAGAATGCTGGAATATTTGTTATGCTATCAAATCTGTATGCTGGTTTTGGCCTGTGGAATGAGATAGAGCATTTGAGGGAATTCGTGAGAAAGGAAGGGCTGTATAAAGGTGTTGGATGTAGCTGGATAGAGATCACAAGTTGA

Coding sequence (CDS)

ATGTCGAGCCCTCACCATTTTCACATTAGACGAACTCTTGATTCCTTCGCCAGTTTCTTCCGCCATTCTTCTCAAATCAAAAATCTTAAAGCCATTAAGAAGATTCACGCCCATTTTCTTAGAACCCACTTCCAATTTGTCTCCCCCAATCTTCACTCTAAGCTTATACTATCCTACTCCCAAATCATCCCCAATTTTGATGCGCAAAGCTTAAACAGCATCTTCAAATGCATCATCCCCAGAAGTTCTTTAACCTTCAACGTAATAATTTCTGACTTTTCTCGACATGGGTTTCCAGATTTTGCGTTAATGGCGTTTTCTTTCATGCATTCTAATGGGGTTCCTATTGACACGTATGCCCTCTGCAGCTCTTTGAGTGCTTGTTCTCTTATTCAGAATGCGGTGTATGGAAAACAGATTCACGCATCCGTGGGGAAATCAGGATGGTTATGTAGTGTTTTTGTGGGGAGTGCGATTGTTGATATGTATGCAAAAAATTCGTTGATTAATGATGCAGTTGAAGTGTTTGATGAAATTCCCATGAAGAATACTGTGTGCGCAAATGCATTGTTGGCTGGCTACGGCGAGGCTAGGATGTGGGTTGAGGGACTTGAATTGCTACGGAAGATGCAGGCTCTGGATTTGAAGTACGACAAGTTCACTCTGTCTGCTTCCTTACGTGCATGCGCTGGTTTATCTGCAATTGCACTAGGTAAGCAAGTGCATGCTTATCTACTGCGAACGGTTTATGATATTACAAGTGATGTTCATCTGCAAAGCACATTAATTGAAATGTATGGGAAGAGTGGCATGGTGGAAAAAGCATGGAATGTCTTCGATTTCGCAGGACTTGGACAAAAAGAAGATGGGAAAAGGGATATTATAATGTGGACTTCTATGCTTAGTGTATATGGTAGAAACGGGTGTTACAAAAGAGTAATTGAACTGTACAACCAGATGTTGATGGAAGGGAACAAACCAGATAGGGTGGCTTTTGTGACAGTCATATCAGCTTGTGGTCATACTGGTGAGGTGAATCTTGGAATCAAGTATTTTGACTCCATGCAGAGTGACTTTGGGTTGGAACCTGGTCAAGAGCACTACAGTTGTCTGGTTGACTTGCTCTGTAGAGCTGGGGAATTGGAAAAAGCATGGAAGCTCGTAAATGATATCAAAAGTCGGGATTACAATATTTCCCTTTGGGGAGCTTTACTCCGTGCTTGCTTGGAACAGGGAAATATTAAGTTGGGTAATTTGGCAGCTCGTAGGGCACTTGAATTGGATCCTCAGAATGCTGGAATATTTGTTATGCTATCAAATCTGTATGCTGGTTTTGGCCTGTGGAATGAGATAGAGCATTTGAGGGAATTCGTGAGAAAGGAAGGGCTGTATAAAGGTGTTGGATGTAGCTGGATAGAGATCACAAGTTGA

Protein sequence

MSSPHHFHIRRTLDSFASFFRHSSQIKNLKAIKKIHAHFLRTHFQFVSPNLHSKLILSYSQIIPNFDAQSLNSIFKCIIPRSSLTFNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYALCSSLSACSLIQNAVYGKQIHASVGKSGWLCSVFVGSAIVDMYAKNSLINDAVEVFDEIPMKNTVCANALLAGYGEARMWVEGLELLRKMQALDLKYDKFTLSASLRACAGLSAIALGKQVHAYLLRTVYDITSDVHLQSTLIEMYGKSGMVEKAWNVFDFAGLGQKEDGKRDIIMWTSMLSVYGRNGCYKRVIELYNQMLMEGNKPDRVAFVTVISACGHTGEVNLGIKYFDSMQSDFGLEPGQEHYSCLVDLLCRAGELEKAWKLVNDIKSRDYNISLWGALLRACLEQGNIKLGNLAARRALELDPQNAGIFVMLSNLYAGFGLWNEIEHLREFVRKEGLYKGVGCSWIEITS
Homology
BLAST of Lsi10G004300 vs. ExPASy Swiss-Prot
Match: Q9SIT7 (Pentatricopeptide repeat-containing protein At2g13600 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E76 PE=3 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 2.4e-68
Identity = 164/497 (33.00%), Postives = 266/497 (53.52%), Query Frame = 0

Query: 15  SFASFFRHSSQIKNLKAIKKIHAHFLRTHFQFVSP-NLHSKLILSYSQIIPNFDAQSLNS 74
           SFAS     S + ++   K +  H L     F+S   + S L+  YS+     DAQ    
Sbjct: 154 SFASVLSACSGLNDMN--KGVQVHSLIAKSPFLSDVYIGSALVDMYSKCGNVNDAQ---R 213

Query: 75  IFKCIIPRSSLTFNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYALCSSLSACSLIQN 134
           +F  +  R+ +++N +I+ F ++G    AL  F  M  + V  D   L S +SAC+ +  
Sbjct: 214 VFDEMGDRNVVSWNSLITCFEQNGPAVEALDVFQMMLESRVEPDEVTLASVISACASLSA 273

Query: 135 AVYGKQIHASVGKSGWLCS-VFVGSAIVDMYAKNSLINDAVEVFDEIPMKNTVCA----- 194
              G+++H  V K+  L + + + +A VDMYAK S I +A  +FD +P++N +       
Sbjct: 274 IKVGQEVHGRVVKNDKLRNDIILSNAFVDMYAKCSRIKEARFIFDSMPIRNVIAETSMIS 333

Query: 195 --------------------------NALLAGYGEARMWVEGLELLRKMQALDLKYDKFT 254
                                     NAL+AGY +     E L L   ++   +    ++
Sbjct: 334 GYAMAASTKAARLMFTKMAERNVVSWNALIAGYTQNGENEEALSLFCLLKRESVCPTHYS 393

Query: 255 LSASLRACAGLSAIALGKQVHAYLLRTVYDITS----DVHLQSTLIEMYGKSGMVEKAWN 314
            +  L+ACA L+ + LG Q H ++L+  +   S    D+ + ++LI+MY K G VE+ + 
Sbjct: 394 FANILKACADLAELHLGMQAHVHVLKHGFKFQSGEEDDIFVGNSLIDMYVKCGCVEEGYL 453

Query: 315 VFDFAGLGQKEDGKRDIIMWTSMLSVYGRNGCYKRVIELYNQMLMEGNKPDRVAFVTVIS 374
           VF       ++  +RD + W +M+  + +NG     +EL+ +ML  G KPD +  + V+S
Sbjct: 454 VF-------RKMMERDCVSWNAMIIGFAQNGYGNEALELFREMLESGEKPDHITMIGVLS 513

Query: 375 ACGHTGEVNLGIKYFDSMQSDFGLEPGQEHYSCLVDLLCRAGELEKAWKLVNDIKSRDYN 434
           ACGH G V  G  YF SM  DFG+ P ++HY+C+VDLL RAG LE+A  ++ ++  +  +
Sbjct: 514 ACGHAGFVEEGRHYFSSMTRDFGVAPLRDHYTCMVDLLGRAGFLEEAKSMIEEMPMQPDS 573

Query: 435 ISLWGALLRACLEQGNIKLGNLAARRALELDPQNAGIFVMLSNLYAGFGLWNEIEHLREF 475
           + +WG+LL AC    NI LG   A + LE++P N+G +V+LSN+YA  G W ++ ++R+ 
Sbjct: 574 V-IWGSLLAACKVHRNITLGKYVAEKLLEVEPSNSGPYVLLSNMYAELGKWEDVMNVRKS 633

BLAST of Lsi10G004300 vs. ExPASy Swiss-Prot
Match: Q9SN39 (Pentatricopeptide repeat-containing protein DOT4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DOT4 PE=2 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 3.1e-68
Identity = 133/409 (32.52%), Postives = 236/409 (57.70%), Query Frame = 0

Query: 67  DAQSLNSIFKCIIPRSSLTFNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYALCSSLS 126
           D  S  ++F+ +  RS +++  +I+ ++R G    A+  F  M   G+  D Y + + L+
Sbjct: 346 DLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLN 405

Query: 127 ACSLIQNAVYGKQIHASVGKSGWLCSVFVGSAIVDMYAKNSLINDAVEVFDEIPMKNTVC 186
            C+  +    GK++H  + ++     +FV +A++DMYAK   + +A  VF E+ +K+ + 
Sbjct: 406 CCARYRLLDEGKRVHEWIKENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIIS 465

Query: 187 ANALLAGYGEARMWVEGLELLR-KMQALDLKYDKFTLSASLRACAGLSAIALGKQVHAYL 246
            N ++ GY +     E L L    ++      D+ T++  L ACA LSA   G+++H Y+
Sbjct: 466 WNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERTVACVLPACASLSAFDKGREIHGYI 525

Query: 247 LRTVYDITSDVHLQSTLIEMYGKSGMVEKAWNVFDFAGLGQKEDGKRDIIMWTSMLSVYG 306
           +R  Y   SD H+ ++L++MY K G +  A  +FD       +   +D++ WT M++ YG
Sbjct: 526 MRNGY--FSDRHVANSLVDMYAKCGALLLAHMLFD-------DIASKDLVSWTVMIAGYG 585

Query: 307 RNGCYKRVIELYNQMLMEGNKPDRVAFVTVISACGHTGEVNLGIKYFDSMQSDFGLEPGQ 366
            +G  K  I L+NQM   G + D ++FV+++ AC H+G V+ G ++F+ M+ +  +EP  
Sbjct: 586 MHGFGKEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWRFFNIMRHECKIEPTV 645

Query: 367 EHYSCLVDLLCRAGELEKAWKLVNDIKSRDYNISLWGALLRACLEQGNIKLGNLAARRAL 426
           EHY+C+VD+L R G+L KA++ + ++     + ++WGALL  C    ++KL    A +  
Sbjct: 646 EHYACIVDMLARTGDLIKAYRFIENMPIPP-DATIWGALLCGCRIHHDVKLAEKVAEKVF 705

Query: 427 ELDPQNAGIFVMLSNLYAGFGLWNEIEHLREFVRKEGLYKGVGCSWIEI 475
           EL+P+N G +V+++N+YA    W +++ LR+ + + GL K  GCSWIEI
Sbjct: 706 ELEPENTGYYVLMANIYAEAEKWEQVKRLRKRIGQRGLRKNPGCSWIEI 744

BLAST of Lsi10G004300 vs. ExPASy Swiss-Prot
Match: Q9LZ19 (Pentatricopeptide repeat-containing protein At5g04780, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PCMP-H16 PE=2 SV=2)

HSP 1 Score: 259.2 bits (661), Expect = 9.0e-68
Identity = 137/401 (34.16%), Postives = 246/401 (61.35%), Query Frame = 0

Query: 74  IFKCIIPRSSLTFNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYALCSSLSACSLIQN 133
           +F  ++ RS +++N +I  ++R+     AL  F  M + G     + + S LSAC +  +
Sbjct: 118 VFDGMLERSLVSWNTMIGLYTRNRMESEALDIFLEMRNEGFKFSEFTISSVLSACGVNCD 177

Query: 134 AVYGKQIHASVGKSGWLCSVFVGSAIVDMYAKNSLINDAVEVFDEIPMKNTVCANALLAG 193
           A+  K++H    K+    +++VG+A++D+YAK  +I DAV+VF+ +  K++V  ++++AG
Sbjct: 178 ALECKKLHCLSVKTCIDLNLYVGTALLDLYAKCGMIKDAVQVFESMQDKSSVTWSSMVAG 237

Query: 194 YGEARMWVEGLELLRKMQALDLKYDKFTLSASLRACAGLSAIALGKQVHAYLLRTVYDIT 253
           Y + + + E L L R+ Q + L+ ++FTLS+ + AC+ L+A+  GKQ+HA + ++ +   
Sbjct: 238 YVQNKNYEEALLLYRRAQRMSLEQNQFTLSSVICACSNLAALIEGKQMHAVICKSGFG-- 297

Query: 254 SDVHLQSTLIEMYGKSGMVEKAWNVFDFAGLGQKEDGKRDIIMWTSMLSVYGRNGCYKRV 313
           S+V + S+ ++MY K G + +++ +F        E  ++++ +W +++S + ++   K V
Sbjct: 298 SNVFVASSAVDMYAKCGSLRESYIIF-------SEVQEKNLELWNTIISGFAKHARPKEV 357

Query: 314 IELYNQMLMEGNKPDRVAFVTVISACGHTGEVNLGIKYFDSMQSDFGLEPGQEHYSCLVD 373
           + L+ +M  +G  P+ V F +++S CGHTG V  G ++F  M++ +GL P   HYSC+VD
Sbjct: 358 MILFEKMQQDGMHPNEVTFSSLLSVCGHTGLVEEGRRFFKLMRTTYGLSPNVVHYSCMVD 417

Query: 374 LLCRAGELEKAWKLVNDIKSRDYNISLWGALLRACLEQGNIKLGNLAARRALELDPQNAG 433
           +L RAG L +A++L+  I   D   S+WG+LL +C    N++L  +AA +  EL+P+NAG
Sbjct: 418 ILGRAGLLSEAYELIKSIPF-DPTASIWGSLLASCRVYKNLELAEVAAEKLFELEPENAG 477

Query: 434 IFVMLSNLYAGFGLWNEIEHLREFVRKEGLYKGVGCSWIEI 475
             V+LSN+YA    W EI   R+ +R   + K  G SWI+I
Sbjct: 478 NHVLLSNIYAANKQWEEIAKSRKLLRDCDVKKVRGKSWIDI 508

BLAST of Lsi10G004300 vs. ExPASy Swiss-Prot
Match: Q9LW63 (Putative pentatricopeptide repeat-containing protein At3g23330 OS=Arabidopsis thaliana OX=3702 GN=PCMP-H32 PE=3 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 9.3e-65
Identity = 135/409 (33.01%), Postives = 229/409 (55.99%), Query Frame = 0

Query: 66  FDAQSLNSIFKCIIPRSSLTFNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYALCSSL 125
           F   S+  +F+ +  +  +++N II+ +++ G  + AL     M +  +  D++ L S L
Sbjct: 190 FGIDSVRRVFEVMPRKDVVSYNTIIAGYAQSGMYEDALRMVREMGTTDLKPDSFTLSSVL 249

Query: 126 SACSLIQNAVYGKQIHASVGKSGWLCSVFVGSAIVDMYAKNSLINDAVEVFDEIPMKNTV 185
              S   + + GK+IH  V + G    V++GS++VDMYAK++ I D+  VF  +  ++ +
Sbjct: 250 PIFSEYVDVIKGKEIHGYVIRKGIDSDVYIGSSLVDMYAKSARIEDSERVFSRLYCRDGI 309

Query: 186 CANALLAGYGEARMWVEGLELLRKMQALDLKYDKFTLSASLRACAGLSAIALGKQVHAYL 245
             N+L+AGY +   + E L L R+M    +K      S+ + ACA L+ + LGKQ+H Y+
Sbjct: 310 SWNSLVAGYVQNGRYNEALRLFRQMVTAKVKPGAVAFSSVIPACAHLATLHLGKQLHGYV 369

Query: 246 LRTVYDITSDVHLQSTLIEMYGKSGMVEKAWNVFDFAGLGQKEDGKRDIIMWTSMLSVYG 305
           LR  +   S++ + S L++MY K G ++ A  +FD   +        D + WT+++  + 
Sbjct: 370 LRGGFG--SNIFIASALVDMYSKCGNIKAARKIFDRMNV-------LDEVSWTAIIMGHA 429

Query: 306 RNGCYKRVIELYNQMLMEGNKPDRVAFVTVISACGHTGEVNLGIKYFDSMQSDFGLEPGQ 365
            +G     + L+ +M  +G KP++VAFV V++AC H G V+    YF+SM   +GL    
Sbjct: 430 LHGHGHEAVSLFEEMKRQGVKPNQVAFVAVLTACSHVGLVDEAWGYFNSMTKVYGLNQEL 489

Query: 366 EHYSCLVDLLCRAGELEKAWKLVNDIKSRDYNISLWGALLRACLEQGNIKLGNLAARRAL 425
           EHY+ + DLL RAG+LE+A+  ++ +       S+W  LL +C    N++L    A +  
Sbjct: 490 EHYAAVADLLGRAGKLEEAYNFISKMCVEPTG-SVWSTLLSSCSVHKNLELAEKVAEKIF 549

Query: 426 ELDPQNAGIFVMLSNLYAGFGLWNEIEHLREFVRKEGLYKGVGCSWIEI 475
            +D +N G +V++ N+YA  G W E+  LR  +RK+GL K   CSWIE+
Sbjct: 550 TVDSENMGAYVLMCNMYASNGRWKEMAKLRLRMRKKGLRKKPACSWIEM 588

BLAST of Lsi10G004300 vs. ExPASy Swiss-Prot
Match: Q9SX45 (Pentatricopeptide repeat-containing protein At1g50270 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E42 PE=2 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 1.2e-64
Identity = 144/442 (32.58%), Postives = 248/442 (56.11%), Query Frame = 0

Query: 34  KIHAHFLRTHFQFVSPNLHSKLILSYSQIIPNFDAQSLNSIFKCIIPRSSLTFNVIISDF 93
           + HAH ++       P + + LI  YS     FD  S   +F     +  +T+  +I  F
Sbjct: 124 QFHAHIVKFGLD-SDPFVRNSLISGYSS-SGLFDFAS--RLFDGAEDKDVVTWTAMIDGF 183

Query: 94  SRHGFPDFALMAFSFMHSNGVPIDTYALCSSLSACSLIQNAVYGKQIHASVGKSGWL-CS 153
            R+G    A++ F  M   GV  +   + S L A   +++  +G+ +H    ++G + C 
Sbjct: 184 VRNGSASEAMVYFVEMKKTGVAANEMTVVSVLKAAGKVEDVRFGRSVHGLYLETGRVKCD 243

Query: 154 VFVGSAIVDMYAKNSLINDAVEVFDEIPMKNTVCANALLAGYGEARMWVEGLELLRKMQA 213
           VF+GS++VDMY K S  +DA +VFDE+P +N V   AL+AGY ++R + +G+ +  +M  
Sbjct: 244 VFIGSSLVDMYGKCSCYDDAQKVFDEMPSRNVVTWTALIAGYVQSRCFDKGMLVFEEMLK 303

Query: 214 LDLKYDKFTLSASLRACAGLSAIALGKQVHAYLLRTVYDITSDVHLQSTLIEMYGKSGMV 273
            D+  ++ TLS+ L ACA + A+  G++VH Y+++   +I +     +TLI++Y K G +
Sbjct: 304 SDVAPNEKTLSSVLSACAHVGALHRGRRVHCYMIKNSIEINTTA--GTTLIDLYVKCGCL 363

Query: 274 EKAWNVFDFAGLGQKEDGKRDIIMWTSMLSVYGRNGCYKRVIELYNQMLMEGNKPDRVAF 333
           E+A  VF+          ++++  WT+M++ +  +G  +   +L+  ML     P+ V F
Sbjct: 364 EEAILVFE-------RLHEKNVYTWTAMINGFAAHGYARDAFDLFYTMLSSHVSPNEVTF 423

Query: 334 VTVISACGHTGEVNLGIKYFDSMQSDFGLEPGQEHYSCLVDLLCRAGELEKAWKLVNDIK 393
           + V+SAC H G V  G + F SM+  F +EP  +HY+C+VDL  R G LE+A  L+  + 
Sbjct: 424 MAVLSACAHGGLVEEGRRLFLSMKGRFNMEPKADHYACMVDLFGRKGLLEEAKALIERMP 483

Query: 394 SRDYNISLWGALLRACLEQGNIKLGNLAARRALELDPQNAGIFVMLSNLYAGFGLWNEIE 453
               N+ +WGAL  +CL   + +LG  AA R ++L P ++G + +L+NLY+    W+E+ 
Sbjct: 484 MEPTNV-VWGALFGSCLLHKDYELGKYAASRVIKLQPSHSGRYTLLANLYSESQNWDEVA 543

Query: 454 HLREFVRKEGLYKGVGCSWIEI 475
            +R+ ++ + + K  G SWIE+
Sbjct: 544 RVRKQMKDQQVVKSPGFSWIEV 551

BLAST of Lsi10G004300 vs. ExPASy TrEMBL
Match: A0A1S3C6K8 (pentatricopeptide repeat-containing protein At2g13600-like OS=Cucumis melo OX=3656 GN=LOC103497272 PE=4 SV=1)

HSP 1 Score: 897.1 bits (2317), Expect = 3.1e-257
Identity = 435/466 (93.35%), Postives = 451/466 (96.78%), Query Frame = 0

Query: 11  RTLDSFASFFRHSSQIKNLKAIKKIHAHFLRTHFQFVSPNLHSKLILSYSQIIPNFDAQS 70
           R+LDSFASFF HSSQIKNLK IKKIHAH LRTHFQF SPNLHSKLILSYSQIIPNFDAQS
Sbjct: 4   RSLDSFASFFGHSSQIKNLKTIKKIHAHLLRTHFQFFSPNLHSKLILSYSQIIPNFDAQS 63

Query: 71  LNSIFKCIIPRSSLTFNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYALCSSLSACSL 130
           LNSIFKCIIPR+SLTFNV+ISDFSRHGFPDFALM FSFMHSNGVPIDTYALCSSL+ CS 
Sbjct: 64  LNSIFKCIIPRTSLTFNVMISDFSRHGFPDFALMGFSFMHSNGVPIDTYALCSSLNGCSF 123

Query: 131 IQNAVYGKQIHASVGKSGWLCSVFVGSAIVDMYAKNSLINDAVEVFDEIPMKNTVCANAL 190
           +QN VYGKQIHA VGKSGWLCSVFVGSAIVDMYAKNSLI DAVEVFDE+PMKNTVCANAL
Sbjct: 124 VQNVVYGKQIHAFVGKSGWLCSVFVGSAIVDMYAKNSLIYDAVEVFDEMPMKNTVCANAL 183

Query: 191 LAGYGEARMWVEGLELLRKMQALDLKYDKFTLSASLRACAGLSAIALGKQVHAYLLRTVY 250
           LAGYGEARMWVEGLELLRKMQ LDLKYD+FTLSASLRACAGLSAIALGKQ+HAY+LRTVY
Sbjct: 184 LAGYGEARMWVEGLELLRKMQGLDLKYDQFTLSASLRACAGLSAIALGKQLHAYILRTVY 243

Query: 251 DITSDVHLQSTLIEMYGKSGMVEKAWNVFDFAGLGQKEDGKRDIIMWTSMLSVYGRNGCY 310
           DITSDVHLQSTL+EMYGK+GMVEKAWNVF+FAGLG +EDGKRDIIMWTSMLSVYGRNGCY
Sbjct: 244 DITSDVHLQSTLVEMYGKNGMVEKAWNVFNFAGLGHREDGKRDIIMWTSMLSVYGRNGCY 303

Query: 311 KRVIELYNQMLMEGNKPDRVAFVTVISACGHTGEVNLGIKYFDSMQSDFGLEPGQEHYSC 370
           KRVIELYNQMLMEG KPDRVAFVTVISACGHTGEVNLG+KYFDSMQSDFGLEPGQEHYSC
Sbjct: 304 KRVIELYNQMLMEGIKPDRVAFVTVISACGHTGEVNLGVKYFDSMQSDFGLEPGQEHYSC 363

Query: 371 LVDLLCRAGELEKAWKLVNDIKSRDYNISLWGALLRACLEQGNIKLGNLAARRALELDPQ 430
           L DLLCRAGELEKAWKLVNDIKSRDYN+SLWGALLRACLEQGNIKLGNLAARRALELDPQ
Sbjct: 364 LADLLCRAGELEKAWKLVNDIKSRDYNVSLWGALLRACLEQGNIKLGNLAARRALELDPQ 423

Query: 431 NAGIFVMLSNLYAGFGLWNEIEHLREFVRKEGLYKGVGCSWIEITS 477
           NAGIF+MLSNLYAGFGLWNEIEHLREFVRKEGLYK VGCSWIEITS
Sbjct: 424 NAGIFIMLSNLYAGFGLWNEIEHLREFVRKEGLYKDVGCSWIEITS 469

BLAST of Lsi10G004300 vs. ExPASy TrEMBL
Match: A0A5D3BKX2 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001930 PE=4 SV=1)

HSP 1 Score: 897.1 bits (2317), Expect = 3.1e-257
Identity = 435/466 (93.35%), Postives = 451/466 (96.78%), Query Frame = 0

Query: 11  RTLDSFASFFRHSSQIKNLKAIKKIHAHFLRTHFQFVSPNLHSKLILSYSQIIPNFDAQS 70
           R+LDSFASFF HSSQIKNLK IKKIHAH LRTHFQF SPNLHSKLILSYSQIIPNFDAQS
Sbjct: 4   RSLDSFASFFGHSSQIKNLKTIKKIHAHLLRTHFQFFSPNLHSKLILSYSQIIPNFDAQS 63

Query: 71  LNSIFKCIIPRSSLTFNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYALCSSLSACSL 130
           LNSIFKCIIPR+SLTFNV+ISDFSRHGFPDFALM FSFMHSNGVPIDTYALCSSL+ CS 
Sbjct: 64  LNSIFKCIIPRTSLTFNVMISDFSRHGFPDFALMGFSFMHSNGVPIDTYALCSSLNGCSF 123

Query: 131 IQNAVYGKQIHASVGKSGWLCSVFVGSAIVDMYAKNSLINDAVEVFDEIPMKNTVCANAL 190
           +QN VYGKQIHA VGKSGWLCSVFVGSAIVDMYAKNSLI DAVEVFDE+PMKNTVCANAL
Sbjct: 124 VQNVVYGKQIHAFVGKSGWLCSVFVGSAIVDMYAKNSLIYDAVEVFDEMPMKNTVCANAL 183

Query: 191 LAGYGEARMWVEGLELLRKMQALDLKYDKFTLSASLRACAGLSAIALGKQVHAYLLRTVY 250
           LAGYGEARMWVEGLELLRKMQ LDLKYD+FTLSASLRACAGLSAIALGKQ+HAY+LRTVY
Sbjct: 184 LAGYGEARMWVEGLELLRKMQGLDLKYDQFTLSASLRACAGLSAIALGKQLHAYILRTVY 243

Query: 251 DITSDVHLQSTLIEMYGKSGMVEKAWNVFDFAGLGQKEDGKRDIIMWTSMLSVYGRNGCY 310
           DITSDVHLQSTL+EMYGK+GMVEKAWNVF+FAGLG +EDGKRDIIMWTSMLSVYGRNGCY
Sbjct: 244 DITSDVHLQSTLVEMYGKNGMVEKAWNVFNFAGLGHREDGKRDIIMWTSMLSVYGRNGCY 303

Query: 311 KRVIELYNQMLMEGNKPDRVAFVTVISACGHTGEVNLGIKYFDSMQSDFGLEPGQEHYSC 370
           KRVIELYNQMLMEG KPDRVAFVTVISACGHTGEVNLG+KYFDSMQSDFGLEPGQEHYSC
Sbjct: 304 KRVIELYNQMLMEGIKPDRVAFVTVISACGHTGEVNLGVKYFDSMQSDFGLEPGQEHYSC 363

Query: 371 LVDLLCRAGELEKAWKLVNDIKSRDYNISLWGALLRACLEQGNIKLGNLAARRALELDPQ 430
           L DLLCRAGELEKAWKLVNDIKSRDYN+SLWGALLRACLEQGNIKLGNLAARRALELDPQ
Sbjct: 364 LADLLCRAGELEKAWKLVNDIKSRDYNVSLWGALLRACLEQGNIKLGNLAARRALELDPQ 423

Query: 431 NAGIFVMLSNLYAGFGLWNEIEHLREFVRKEGLYKGVGCSWIEITS 477
           NAGIF+MLSNLYAGFGLWNEIEHLREFVRKEGLYK VGCSWIEITS
Sbjct: 424 NAGIFIMLSNLYAGFGLWNEIEHLREFVRKEGLYKDVGCSWIEITS 469

BLAST of Lsi10G004300 vs. ExPASy TrEMBL
Match: A0A0A0LLD6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G258600 PE=4 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 3.4e-251
Identity = 426/466 (91.42%), Postives = 443/466 (95.06%), Query Frame = 0

Query: 11  RTLDSFASFFRHSSQIKNLKAIKKIHAHFLRTHFQFVSPNLHSKLILSYSQIIPNFDAQS 70
           R+LDSFASFF HSSQ KNLK IKKIHAH LRTHFQF SPNLHSKLILSYSQIIPNFDA+S
Sbjct: 4   RSLDSFASFFHHSSQFKNLKTIKKIHAHLLRTHFQFFSPNLHSKLILSYSQIIPNFDAKS 63

Query: 71  LNSIFKCIIPRSSLTFNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYALCSSLSACSL 130
           LN IF+CIIPR+SLTFNV+ISDFSRHGF DFALM FSFMHSNGVPIDTYALCSSL+ CS 
Sbjct: 64  LNGIFQCIIPRTSLTFNVMISDFSRHGFQDFALMGFSFMHSNGVPIDTYALCSSLNGCSF 123

Query: 131 IQNAVYGKQIHASVGKSGWLCSVFVGSAIVDMYAKNSLINDAVEVFDEIPMKNTVCANAL 190
           +QN VYGKQIHA VGKSGWLCSVFVGSAIVDMYAKNSLI DAVEVFDE+PMKNTVCANAL
Sbjct: 124 VQNVVYGKQIHAFVGKSGWLCSVFVGSAIVDMYAKNSLIYDAVEVFDEMPMKNTVCANAL 183

Query: 191 LAGYGEARMWVEGLELLRKMQALDLKYDKFTLSASLRACAGLSAIALGKQVHAYLLRTVY 250
           LAGYGEARMWVEGLELLRKMQ LDLKYD+FTLSASLRAC GLSAIALGKQVHAY+LRTVY
Sbjct: 184 LAGYGEARMWVEGLELLRKMQGLDLKYDQFTLSASLRACTGLSAIALGKQVHAYILRTVY 243

Query: 251 DITSDVHLQSTLIEMYGKSGMVEKAWNVFDFAGLGQKEDGKRDIIMWTSMLSVYGRNGCY 310
           DITSDVHLQSTL+EMYGK+GMVEKAWNVF FAGLG++EDGKRDIIMWTSMLSVYGRNGCY
Sbjct: 244 DITSDVHLQSTLVEMYGKNGMVEKAWNVFKFAGLGRQEDGKRDIIMWTSMLSVYGRNGCY 303

Query: 311 KRVIELYNQMLMEGNKPDRVAFVTVISACGHTGEVNLGIKYFDSMQSDFGLEPGQEHYSC 370
           KRVIELYNQMLMEG KPDRVAFVTVISACGHTGEVNLG++YFDSMQSDFGLEPGQEHYSC
Sbjct: 304 KRVIELYNQMLMEGIKPDRVAFVTVISACGHTGEVNLGVQYFDSMQSDFGLEPGQEHYSC 363

Query: 371 LVDLLCRAGELEKAWKLVNDIKSRDYNISLWGALLRACLEQGNIKLGNLAARRALELDPQ 430
           L DLLCRAGELEKAWKLVNDIKSRDYN+SLWGALLRACLEQGNIKLGNLAARRALELDPQ
Sbjct: 364 LADLLCRAGELEKAWKLVNDIKSRDYNVSLWGALLRACLEQGNIKLGNLAARRALELDPQ 423

Query: 431 NAGIFVMLSNLYAGFGLWNEIEHLREFVRKEGLYKGVGCSWIEITS 477
           N GI VMLSNLYAGFGLWNEIEHLRE VRKEGLYK V CSWIEITS
Sbjct: 424 NTGILVMLSNLYAGFGLWNEIEHLRESVRKEGLYKDVACSWIEITS 469

BLAST of Lsi10G004300 vs. ExPASy TrEMBL
Match: A0A6J1FYF9 (pentatricopeptide repeat-containing protein At1g50270-like OS=Cucurbita moschata OX=3662 GN=LOC111448605 PE=4 SV=1)

HSP 1 Score: 873.2 bits (2255), Expect = 4.8e-250
Identity = 425/476 (89.29%), Postives = 450/476 (94.54%), Query Frame = 0

Query: 1   MSSPHHFHIRRTLDSFASFFRHSSQIKNLKAIKKIHAHFLRTHFQFVSPNLHSKLILSYS 60
           MSSPHHFHIRR+LDSFASF RH+SQI+NLKAIKK+HA  LRTHFQF SP+LHSKLILSYS
Sbjct: 1   MSSPHHFHIRRSLDSFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYS 60

Query: 61  QIIPNFDAQSLNSIFKCIIPRSSLTFNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYA 120
           QIIPNFDA SL+S FKCIIPRSSLTFNV+ISDF RHGFPDF+LMA SFMHSNGVPIDTYA
Sbjct: 61  QIIPNFDALSLSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYA 120

Query: 121 LCSSLSACSLIQNAVYGKQIHASVGKSGWLCSVFVGSAIVDMYAKNSLINDAVEVFDEIP 180
           LCSSLSACSL+QN VYGKQ+HA VGKSGWLCSVFVGSAIVDMYAKNSLINDAV+VFDEIP
Sbjct: 121 LCSSLSACSLVQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIP 180

Query: 181 MKNTVCANALLAGYGEARMWVEGLELLRKMQALDLKYDKFTLSASLRACAGLSAIALGKQ 240
           +KNTVCANALLAGYG+A MW++GLELLRKM  LDLKYD+FTLSASLRAC GLSAIALGKQ
Sbjct: 181 VKNTVCANALLAGYGDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQ 240

Query: 241 VHAYLLRTVYDITSDVHLQSTLIEMYGKSGMVEKAWNVFDFAGLGQKEDGKRDIIMWTSM 300
           VHAYLLRTVYDI+SDVHLQSTLIEMYGKSGMVEKAWNVF FAGLGQKEDGKRDIIMWTSM
Sbjct: 241 VHAYLLRTVYDISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSM 300

Query: 301 LSVYGRNGCYKRVIELYNQMLMEGNKPDRVAFVTVISACGHTGEVNLGIKYFDSMQSDFG 360
           LS YGRNGCY RVIELYNQMLMEG KPDRVAFVTVISACGHTG+VNLG+KYFDSMQS FG
Sbjct: 301 LSAYGRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFG 360

Query: 361 LEPGQEHYSCLVDLLCRAGELEKAWKLVNDIKSRDYNISLWGALLRACLEQGNIKLGNLA 420
           LEPGQEHYSCLVDLLCRAGELEKAWKLV+ +KS DYN+ LWGALLRACLE GNIKLGNLA
Sbjct: 361 LEPGQEHYSCLVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLA 420

Query: 421 ARRALELDPQNAGIFVMLSNLYAGFGLWNEIEHLREFVRKEGLYKGVGCSWIEITS 477
           ARRALELDP NAGIFVMLSNLY+GFGLWNEIEHLR FVR+EGL+KGVGCSWIE TS
Sbjct: 421 ARRALELDPTNAGIFVMLSNLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIETTS 476

BLAST of Lsi10G004300 vs. ExPASy TrEMBL
Match: A0A6J1CYG8 (pentatricopeptide repeat-containing protein At4g21300-like OS=Momordica charantia OX=3673 GN=LOC111015438 PE=4 SV=1)

HSP 1 Score: 818.1 bits (2112), Expect = 1.8e-233
Identity = 398/476 (83.61%), Postives = 437/476 (91.81%), Query Frame = 0

Query: 1   MSSPHHFHIRRTLDSFASFFRHSSQIKNLKAIKKIHAHFLRTHFQFVSPNLHSKLILSYS 60
           MS  HHFHI+++LD F+SF RHS++ KNL  IKK+HAH +RTHFQF SP+LH+KLILSYS
Sbjct: 1   MSRSHHFHIKQSLDFFSSFLRHSTETKNLTPIKKLHAHLIRTHFQFFSPSLHTKLILSYS 60

Query: 61  QIIPNFDAQSLNSIFKCIIPRSSLTFNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYA 120
           +  PNF AQSL+S+FK IIPRSSLTFN+II+DF R+G  DFALMAFSFMHSNGVPID+YA
Sbjct: 61  RANPNFGAQSLSSLFKFIIPRSSLTFNIIIADFCRNGSADFALMAFSFMHSNGVPIDSYA 120

Query: 121 LCSSLSACSLIQNAVYGKQIHASVGKSGWLCSVFVGSAIVDMYAKNSLINDAVEVFDEIP 180
           LCS+LSACSL+QN ++GKQ+HA VGKSGWL SVFVGSAIVD+YAKN LI DAVEVFDEIP
Sbjct: 121 LCSTLSACSLVQNVMFGKQMHAFVGKSGWLSSVFVGSAIVDLYAKNGLIGDAVEVFDEIP 180

Query: 181 MKNTVCANALLAGYGEARMWVEGLELLRKMQALDLKYDKFTLSASLRACAGLSAIALGKQ 240
           MKNTVCANALLAGYGEA+MWVEGLELLRKM  LDLKYD FTLSASLR CA LSAIALGKQ
Sbjct: 181 MKNTVCANALLAGYGEAKMWVEGLELLRKMYVLDLKYDHFTLSASLRTCACLSAIALGKQ 240

Query: 241 VHAYLLRTVYDITSDVHLQSTLIEMYGKSGMVEKAWNVFDFAGLGQKEDGKRDIIMWTSM 300
           VHAYLLR +YDI SDVHLQSTLIEMYGKSGMVEKAWNVF+FAGL QKEDGKRDII+WTS+
Sbjct: 241 VHAYLLRMIYDIPSDVHLQSTLIEMYGKSGMVEKAWNVFNFAGLEQKEDGKRDIIVWTSI 300

Query: 301 LSVYGRNGCYKRVIELYNQMLMEGNKPDRVAFVTVISACGHTGEVNLGIKYFDSMQSDFG 360
           LSVYGRNGCYKRVIELY+QMLMEG KPDRVAFVTVISACGHTGEV+LGIKYFDSMQSDFG
Sbjct: 301 LSVYGRNGCYKRVIELYSQMLMEGIKPDRVAFVTVISACGHTGEVDLGIKYFDSMQSDFG 360

Query: 361 LEPGQEHYSCLVDLLCRAGELEKAWKLVNDIKSRDYNISLWGALLRACLEQGNIKLGNLA 420
           L+PGQEHYSCLVDLLCRAGEL+KAWKLVND+KS+  N+SLWGALLRACLE G I+LGNLA
Sbjct: 361 LQPGQEHYSCLVDLLCRAGELDKAWKLVNDMKSQHCNVSLWGALLRACLEHGKIELGNLA 420

Query: 421 ARRALELDPQNAGIFVMLSNLYAGFGLWNEIEHLREFVRKEGLYKGVGCSWIEITS 477
           A++ALELDPQN GIFVMLSNLYA FGLWNEIEHLREFV KEG+ K VGCSWIEITS
Sbjct: 421 AQKALELDPQNTGIFVMLSNLYAIFGLWNEIEHLREFVSKEGMCKDVGCSWIEITS 476

BLAST of Lsi10G004300 vs. NCBI nr
Match: XP_038901137.1 (pentatricopeptide repeat-containing protein At5g04780, mitochondrial-like [Benincasa hispida] >XP_038901138.1 pentatricopeptide repeat-containing protein At5g04780, mitochondrial-like [Benincasa hispida] >XP_038901139.1 pentatricopeptide repeat-containing protein At5g04780, mitochondrial-like [Benincasa hispida] >XP_038901140.1 pentatricopeptide repeat-containing protein At5g04780, mitochondrial-like [Benincasa hispida] >XP_038901141.1 pentatricopeptide repeat-containing protein At5g04780, mitochondrial-like [Benincasa hispida] >XP_038901143.1 pentatricopeptide repeat-containing protein At5g04780, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 914.4 bits (2362), Expect = 3.9e-262
Identity = 449/476 (94.33%), Postives = 460/476 (96.64%), Query Frame = 0

Query: 1   MSSPHHFHIRRTLDSFASFFRHSSQIKNLKAIKKIHAHFLRTHFQFVSPNLHSKLILSYS 60
           MSSPHHF+IRR+LDSFASFFRHS Q KNLKAIK+IHAH LRT FQF SPNLHSKLILSYS
Sbjct: 1   MSSPHHFYIRRSLDSFASFFRHSCQSKNLKAIKRIHAHLLRTRFQFFSPNLHSKLILSYS 60

Query: 61  QIIPNFDAQSLNSIFKCIIPRSSLTFNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYA 120
           QI PNFDAQSLNSIFKCIIPR SL FNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYA
Sbjct: 61  QINPNFDAQSLNSIFKCIIPRRSLNFNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYA 120

Query: 121 LCSSLSACSLIQNAVYGKQIHASVGKSGWLCSVFVGSAIVDMYAKNSLINDAVEVFDEIP 180
           LCSSL++CSL+QN VYGKQIHA VGKSGWLCSVFVGSAIVDMYAKNSLINDAVEVFDEIP
Sbjct: 121 LCSSLTSCSLVQNGVYGKQIHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVEVFDEIP 180

Query: 181 MKNTVCANALLAGYGEARMWVEGLELLRKMQALDLKYDKFTLSASLRACAGLSAIALGKQ 240
           MKNTVCANALLAGYGEARMWVEGLELLR+MQALDLKYD+FTLSASLRACAGLSAIALGKQ
Sbjct: 181 MKNTVCANALLAGYGEARMWVEGLELLREMQALDLKYDQFTLSASLRACAGLSAIALGKQ 240

Query: 241 VHAYLLRTVYDITSDVHLQSTLIEMYGKSGMVEKAWNVFDFAGLGQKEDGKRDIIMWTSM 300
           VHAYLLRTVYDIT DVHLQSTLIEMYGKSGMVEKAWNVF+FAGL QKEDGKRDIIMWTSM
Sbjct: 241 VHAYLLRTVYDITCDVHLQSTLIEMYGKSGMVEKAWNVFNFAGLRQKEDGKRDIIMWTSM 300

Query: 301 LSVYGRNGCYKRVIELYNQMLMEGNKPDRVAFVTVISACGHTGEVNLGIKYFDSMQSDFG 360
           LSVYGRNGCYKRVIELYNQMLMEGNKPDRVAFVTVISACGHTGEVNLG+KYFDSMQSDFG
Sbjct: 301 LSVYGRNGCYKRVIELYNQMLMEGNKPDRVAFVTVISACGHTGEVNLGVKYFDSMQSDFG 360

Query: 361 LEPGQEHYSCLVDLLCRAGELEKAWKLVNDIKSRDYNISLWGALLRACLEQGNIKLGNLA 420
           LEPGQEHYSCLVDLLCRAGELEKAWKLVNDIKS DYN+SLWGALLRACLEQGNIKLGNLA
Sbjct: 361 LEPGQEHYSCLVDLLCRAGELEKAWKLVNDIKSWDYNVSLWGALLRACLEQGNIKLGNLA 420

Query: 421 ARRALELDPQNAGIFVMLSNLYAGFGLWNEIEHLREFVRKEGLYKGVGCSWIEITS 477
           A RALELDPQNAGIFVMLSNLYAGFGLWNEIE LREFVRKEGLYK VGCSWIEITS
Sbjct: 421 AHRALELDPQNAGIFVMLSNLYAGFGLWNEIERLREFVRKEGLYKDVGCSWIEITS 476

BLAST of Lsi10G004300 vs. NCBI nr
Match: XP_008457622.1 (PREDICTED: pentatricopeptide repeat-containing protein At2g13600-like [Cucumis melo] >XP_008457623.1 PREDICTED: pentatricopeptide repeat-containing protein At2g13600-like [Cucumis melo] >XP_016902170.1 PREDICTED: pentatricopeptide repeat-containing protein At2g13600-like [Cucumis melo] >KAA0045664.1 pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa] >TYJ99619.1 pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 897.1 bits (2317), Expect = 6.5e-257
Identity = 435/466 (93.35%), Postives = 451/466 (96.78%), Query Frame = 0

Query: 11  RTLDSFASFFRHSSQIKNLKAIKKIHAHFLRTHFQFVSPNLHSKLILSYSQIIPNFDAQS 70
           R+LDSFASFF HSSQIKNLK IKKIHAH LRTHFQF SPNLHSKLILSYSQIIPNFDAQS
Sbjct: 4   RSLDSFASFFGHSSQIKNLKTIKKIHAHLLRTHFQFFSPNLHSKLILSYSQIIPNFDAQS 63

Query: 71  LNSIFKCIIPRSSLTFNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYALCSSLSACSL 130
           LNSIFKCIIPR+SLTFNV+ISDFSRHGFPDFALM FSFMHSNGVPIDTYALCSSL+ CS 
Sbjct: 64  LNSIFKCIIPRTSLTFNVMISDFSRHGFPDFALMGFSFMHSNGVPIDTYALCSSLNGCSF 123

Query: 131 IQNAVYGKQIHASVGKSGWLCSVFVGSAIVDMYAKNSLINDAVEVFDEIPMKNTVCANAL 190
           +QN VYGKQIHA VGKSGWLCSVFVGSAIVDMYAKNSLI DAVEVFDE+PMKNTVCANAL
Sbjct: 124 VQNVVYGKQIHAFVGKSGWLCSVFVGSAIVDMYAKNSLIYDAVEVFDEMPMKNTVCANAL 183

Query: 191 LAGYGEARMWVEGLELLRKMQALDLKYDKFTLSASLRACAGLSAIALGKQVHAYLLRTVY 250
           LAGYGEARMWVEGLELLRKMQ LDLKYD+FTLSASLRACAGLSAIALGKQ+HAY+LRTVY
Sbjct: 184 LAGYGEARMWVEGLELLRKMQGLDLKYDQFTLSASLRACAGLSAIALGKQLHAYILRTVY 243

Query: 251 DITSDVHLQSTLIEMYGKSGMVEKAWNVFDFAGLGQKEDGKRDIIMWTSMLSVYGRNGCY 310
           DITSDVHLQSTL+EMYGK+GMVEKAWNVF+FAGLG +EDGKRDIIMWTSMLSVYGRNGCY
Sbjct: 244 DITSDVHLQSTLVEMYGKNGMVEKAWNVFNFAGLGHREDGKRDIIMWTSMLSVYGRNGCY 303

Query: 311 KRVIELYNQMLMEGNKPDRVAFVTVISACGHTGEVNLGIKYFDSMQSDFGLEPGQEHYSC 370
           KRVIELYNQMLMEG KPDRVAFVTVISACGHTGEVNLG+KYFDSMQSDFGLEPGQEHYSC
Sbjct: 304 KRVIELYNQMLMEGIKPDRVAFVTVISACGHTGEVNLGVKYFDSMQSDFGLEPGQEHYSC 363

Query: 371 LVDLLCRAGELEKAWKLVNDIKSRDYNISLWGALLRACLEQGNIKLGNLAARRALELDPQ 430
           L DLLCRAGELEKAWKLVNDIKSRDYN+SLWGALLRACLEQGNIKLGNLAARRALELDPQ
Sbjct: 364 LADLLCRAGELEKAWKLVNDIKSRDYNVSLWGALLRACLEQGNIKLGNLAARRALELDPQ 423

Query: 431 NAGIFVMLSNLYAGFGLWNEIEHLREFVRKEGLYKGVGCSWIEITS 477
           NAGIF+MLSNLYAGFGLWNEIEHLREFVRKEGLYK VGCSWIEITS
Sbjct: 424 NAGIFIMLSNLYAGFGLWNEIEHLREFVRKEGLYKDVGCSWIEITS 469

BLAST of Lsi10G004300 vs. NCBI nr
Match: KAG6571067.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 875.9 bits (2262), Expect = 1.5e-250
Identity = 426/476 (89.50%), Postives = 451/476 (94.75%), Query Frame = 0

Query: 1   MSSPHHFHIRRTLDSFASFFRHSSQIKNLKAIKKIHAHFLRTHFQFVSPNLHSKLILSYS 60
           MSSPHHFHIRR+LDSFASF RH+SQI+NLKAIKK+HA  LRTHFQF SP+LHSKLILSYS
Sbjct: 1   MSSPHHFHIRRSLDSFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYS 60

Query: 61  QIIPNFDAQSLNSIFKCIIPRSSLTFNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYA 120
           QIIPNFDA SL+S FKCIIPRSSLTFNV+ISDF RHGFPDF+LMA SFMHSNGVPIDTYA
Sbjct: 61  QIIPNFDALSLSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYA 120

Query: 121 LCSSLSACSLIQNAVYGKQIHASVGKSGWLCSVFVGSAIVDMYAKNSLINDAVEVFDEIP 180
           LCSSLSACSL+QN VYGKQ+HA VGKSGWLCSVFVGSAIVDMYAKNSLINDAV+VFDEIP
Sbjct: 121 LCSSLSACSLVQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIP 180

Query: 181 MKNTVCANALLAGYGEARMWVEGLELLRKMQALDLKYDKFTLSASLRACAGLSAIALGKQ 240
           +KNTVCANALLAGYG+A MW++GLELLRKM  LDLKYD+FTLSASLRAC GLSAIALGKQ
Sbjct: 181 VKNTVCANALLAGYGDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQ 240

Query: 241 VHAYLLRTVYDITSDVHLQSTLIEMYGKSGMVEKAWNVFDFAGLGQKEDGKRDIIMWTSM 300
           VHAYLLRTVYDI+SDVHLQSTLIEMYGKSGMVEKAWNVF FAGLGQKEDGKRDIIMWTSM
Sbjct: 241 VHAYLLRTVYDISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSM 300

Query: 301 LSVYGRNGCYKRVIELYNQMLMEGNKPDRVAFVTVISACGHTGEVNLGIKYFDSMQSDFG 360
           LS YGRNGCY RVIELYNQMLMEG KPDRVAFVTVISACGHTG+VNLG+KYFDSMQS FG
Sbjct: 301 LSAYGRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFG 360

Query: 361 LEPGQEHYSCLVDLLCRAGELEKAWKLVNDIKSRDYNISLWGALLRACLEQGNIKLGNLA 420
           LEPGQEHYSCLVDLLCRAGELEKAWKLV+ +KS DYN+ LWGALLRACLE GNIKLGNLA
Sbjct: 361 LEPGQEHYSCLVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLA 420

Query: 421 ARRALELDPQNAGIFVMLSNLYAGFGLWNEIEHLREFVRKEGLYKGVGCSWIEITS 477
           ARRALELDP NAGIFVMLSNLY+GFGLWNEIEHLREFVR+EGL+KGVGCSWIE TS
Sbjct: 421 ARRALELDPTNAGIFVMLSNLYSGFGLWNEIEHLREFVRREGLHKGVGCSWIETTS 476

BLAST of Lsi10G004300 vs. NCBI nr
Match: XP_022944055.1 (pentatricopeptide repeat-containing protein At1g50270-like [Cucurbita moschata] >KAG7010880.1 Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 873.2 bits (2255), Expect = 1.0e-249
Identity = 425/476 (89.29%), Postives = 450/476 (94.54%), Query Frame = 0

Query: 1   MSSPHHFHIRRTLDSFASFFRHSSQIKNLKAIKKIHAHFLRTHFQFVSPNLHSKLILSYS 60
           MSSPHHFHIRR+LDSFASF RH+SQI+NLKAIKK+HA  LRTHFQF SP+LHSKLILSYS
Sbjct: 1   MSSPHHFHIRRSLDSFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYS 60

Query: 61  QIIPNFDAQSLNSIFKCIIPRSSLTFNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYA 120
           QIIPNFDA SL+S FKCIIPRSSLTFNV+ISDF RHGFPDF+LMA SFMHSNGVPIDTYA
Sbjct: 61  QIIPNFDALSLSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYA 120

Query: 121 LCSSLSACSLIQNAVYGKQIHASVGKSGWLCSVFVGSAIVDMYAKNSLINDAVEVFDEIP 180
           LCSSLSACSL+QN VYGKQ+HA VGKSGWLCSVFVGSAIVDMYAKNSLINDAV+VFDEIP
Sbjct: 121 LCSSLSACSLVQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIP 180

Query: 181 MKNTVCANALLAGYGEARMWVEGLELLRKMQALDLKYDKFTLSASLRACAGLSAIALGKQ 240
           +KNTVCANALLAGYG+A MW++GLELLRKM  LDLKYD+FTLSASLRAC GLSAIALGKQ
Sbjct: 181 VKNTVCANALLAGYGDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQ 240

Query: 241 VHAYLLRTVYDITSDVHLQSTLIEMYGKSGMVEKAWNVFDFAGLGQKEDGKRDIIMWTSM 300
           VHAYLLRTVYDI+SDVHLQSTLIEMYGKSGMVEKAWNVF FAGLGQKEDGKRDIIMWTSM
Sbjct: 241 VHAYLLRTVYDISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSM 300

Query: 301 LSVYGRNGCYKRVIELYNQMLMEGNKPDRVAFVTVISACGHTGEVNLGIKYFDSMQSDFG 360
           LS YGRNGCY RVIELYNQMLMEG KPDRVAFVTVISACGHTG+VNLG+KYFDSMQS FG
Sbjct: 301 LSAYGRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFG 360

Query: 361 LEPGQEHYSCLVDLLCRAGELEKAWKLVNDIKSRDYNISLWGALLRACLEQGNIKLGNLA 420
           LEPGQEHYSCLVDLLCRAGELEKAWKLV+ +KS DYN+ LWGALLRACLE GNIKLGNLA
Sbjct: 361 LEPGQEHYSCLVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLA 420

Query: 421 ARRALELDPQNAGIFVMLSNLYAGFGLWNEIEHLREFVRKEGLYKGVGCSWIEITS 477
           ARRALELDP NAGIFVMLSNLY+GFGLWNEIEHLR FVR+EGL+KGVGCSWIE TS
Sbjct: 421 ARRALELDPTNAGIFVMLSNLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIETTS 476

BLAST of Lsi10G004300 vs. NCBI nr
Match: XP_023512242.1 (pentatricopeptide repeat-containing protein At2g13600-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 870.9 bits (2249), Expect = 5.0e-249
Identity = 423/476 (88.87%), Postives = 451/476 (94.75%), Query Frame = 0

Query: 1   MSSPHHFHIRRTLDSFASFFRHSSQIKNLKAIKKIHAHFLRTHFQFVSPNLHSKLILSYS 60
           MS+ HHFHIRR+LDSFASF RHSSQI+NLKAIKK+HA  LRTHFQF SP+LHSKLILSYS
Sbjct: 14  MSNLHHFHIRRSLDSFASFLRHSSQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYS 73

Query: 61  QIIPNFDAQSLNSIFKCIIPRSSLTFNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYA 120
           QI+PNFDAQSL+S FKCIIPRSSLTFNV+ISDF RHGFPDF+L+A SFMHSNGVPIDTYA
Sbjct: 74  QIVPNFDAQSLSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLIALSFMHSNGVPIDTYA 133

Query: 121 LCSSLSACSLIQNAVYGKQIHASVGKSGWLCSVFVGSAIVDMYAKNSLINDAVEVFDEIP 180
           LCSSLSACSL+QNAVYG+Q+HA VGKSGWLCSVFVGSAIVDMYAKNSLINDAV+VFDEIP
Sbjct: 134 LCSSLSACSLVQNAVYGQQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIP 193

Query: 181 MKNTVCANALLAGYGEARMWVEGLELLRKMQALDLKYDKFTLSASLRACAGLSAIALGKQ 240
           +KNTVCANALLAGYG+A MW++GLELLRKM  L+LKYD+FTLSASLRAC GLSAIALGKQ
Sbjct: 194 VKNTVCANALLAGYGDAGMWIDGLELLRKMHVLNLKYDQFTLSASLRACTGLSAIALGKQ 253

Query: 241 VHAYLLRTVYDITSDVHLQSTLIEMYGKSGMVEKAWNVFDFAGLGQKEDGKRDIIMWTSM 300
           VHAYLLRTVYDI+SDVHLQSTLIEMYGKSGMVEKAWNVF FAGLG KEDGKRDIIMWTSM
Sbjct: 254 VHAYLLRTVYDISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGHKEDGKRDIIMWTSM 313

Query: 301 LSVYGRNGCYKRVIELYNQMLMEGNKPDRVAFVTVISACGHTGEVNLGIKYFDSMQSDFG 360
           LS YGRNGCY RVIELYNQMLMEG KPDRVAFVTVISACGHTG+VNLG+KYFDSMQS FG
Sbjct: 314 LSAYGRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFG 373

Query: 361 LEPGQEHYSCLVDLLCRAGELEKAWKLVNDIKSRDYNISLWGALLRACLEQGNIKLGNLA 420
           LEPGQEHYSCLVDLLCRAGEL+KAWKLV+DIKS D+N+ LWGALLRACLE GNIKLGNLA
Sbjct: 374 LEPGQEHYSCLVDLLCRAGELDKAWKLVSDIKSSDHNVPLWGALLRACLEHGNIKLGNLA 433

Query: 421 ARRALELDPQNAGIFVMLSNLYAGFGLWNEIEHLREFVRKEGLYKGVGCSWIEITS 477
           AR ALELDP NAGIFVMLSNLYAGFGLWNEIEHLREFVR+EGLYKGVGCSWIE TS
Sbjct: 434 ARMALELDPTNAGIFVMLSNLYAGFGLWNEIEHLREFVRREGLYKGVGCSWIETTS 489

BLAST of Lsi10G004300 vs. TAIR 10
Match: AT2G13600.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 261.2 bits (666), Expect = 1.7e-69
Identity = 164/497 (33.00%), Postives = 266/497 (53.52%), Query Frame = 0

Query: 15  SFASFFRHSSQIKNLKAIKKIHAHFLRTHFQFVSP-NLHSKLILSYSQIIPNFDAQSLNS 74
           SFAS     S + ++   K +  H L     F+S   + S L+  YS+     DAQ    
Sbjct: 154 SFASVLSACSGLNDMN--KGVQVHSLIAKSPFLSDVYIGSALVDMYSKCGNVNDAQ---R 213

Query: 75  IFKCIIPRSSLTFNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYALCSSLSACSLIQN 134
           +F  +  R+ +++N +I+ F ++G    AL  F  M  + V  D   L S +SAC+ +  
Sbjct: 214 VFDEMGDRNVVSWNSLITCFEQNGPAVEALDVFQMMLESRVEPDEVTLASVISACASLSA 273

Query: 135 AVYGKQIHASVGKSGWLCS-VFVGSAIVDMYAKNSLINDAVEVFDEIPMKNTVCA----- 194
              G+++H  V K+  L + + + +A VDMYAK S I +A  +FD +P++N +       
Sbjct: 274 IKVGQEVHGRVVKNDKLRNDIILSNAFVDMYAKCSRIKEARFIFDSMPIRNVIAETSMIS 333

Query: 195 --------------------------NALLAGYGEARMWVEGLELLRKMQALDLKYDKFT 254
                                     NAL+AGY +     E L L   ++   +    ++
Sbjct: 334 GYAMAASTKAARLMFTKMAERNVVSWNALIAGYTQNGENEEALSLFCLLKRESVCPTHYS 393

Query: 255 LSASLRACAGLSAIALGKQVHAYLLRTVYDITS----DVHLQSTLIEMYGKSGMVEKAWN 314
            +  L+ACA L+ + LG Q H ++L+  +   S    D+ + ++LI+MY K G VE+ + 
Sbjct: 394 FANILKACADLAELHLGMQAHVHVLKHGFKFQSGEEDDIFVGNSLIDMYVKCGCVEEGYL 453

Query: 315 VFDFAGLGQKEDGKRDIIMWTSMLSVYGRNGCYKRVIELYNQMLMEGNKPDRVAFVTVIS 374
           VF       ++  +RD + W +M+  + +NG     +EL+ +ML  G KPD +  + V+S
Sbjct: 454 VF-------RKMMERDCVSWNAMIIGFAQNGYGNEALELFREMLESGEKPDHITMIGVLS 513

Query: 375 ACGHTGEVNLGIKYFDSMQSDFGLEPGQEHYSCLVDLLCRAGELEKAWKLVNDIKSRDYN 434
           ACGH G V  G  YF SM  DFG+ P ++HY+C+VDLL RAG LE+A  ++ ++  +  +
Sbjct: 514 ACGHAGFVEEGRHYFSSMTRDFGVAPLRDHYTCMVDLLGRAGFLEEAKSMIEEMPMQPDS 573

Query: 435 ISLWGALLRACLEQGNIKLGNLAARRALELDPQNAGIFVMLSNLYAGFGLWNEIEHLREF 475
           + +WG+LL AC    NI LG   A + LE++P N+G +V+LSN+YA  G W ++ ++R+ 
Sbjct: 574 V-IWGSLLAACKVHRNITLGKYVAEKLLEVEPSNSGPYVLLSNMYAELGKWEDVMNVRKS 633

BLAST of Lsi10G004300 vs. TAIR 10
Match: AT4G18750.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 260.8 bits (665), Expect = 2.2e-69
Identity = 133/409 (32.52%), Postives = 236/409 (57.70%), Query Frame = 0

Query: 67  DAQSLNSIFKCIIPRSSLTFNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYALCSSLS 126
           D  S  ++F+ +  RS +++  +I+ ++R G    A+  F  M   G+  D Y + + L+
Sbjct: 346 DLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLN 405

Query: 127 ACSLIQNAVYGKQIHASVGKSGWLCSVFVGSAIVDMYAKNSLINDAVEVFDEIPMKNTVC 186
            C+  +    GK++H  + ++     +FV +A++DMYAK   + +A  VF E+ +K+ + 
Sbjct: 406 CCARYRLLDEGKRVHEWIKENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIIS 465

Query: 187 ANALLAGYGEARMWVEGLELLR-KMQALDLKYDKFTLSASLRACAGLSAIALGKQVHAYL 246
            N ++ GY +     E L L    ++      D+ T++  L ACA LSA   G+++H Y+
Sbjct: 466 WNTIIGGYSKNCYANEALSLFNLLLEEKRFSPDERTVACVLPACASLSAFDKGREIHGYI 525

Query: 247 LRTVYDITSDVHLQSTLIEMYGKSGMVEKAWNVFDFAGLGQKEDGKRDIIMWTSMLSVYG 306
           +R  Y   SD H+ ++L++MY K G +  A  +FD       +   +D++ WT M++ YG
Sbjct: 526 MRNGY--FSDRHVANSLVDMYAKCGALLLAHMLFD-------DIASKDLVSWTVMIAGYG 585

Query: 307 RNGCYKRVIELYNQMLMEGNKPDRVAFVTVISACGHTGEVNLGIKYFDSMQSDFGLEPGQ 366
            +G  K  I L+NQM   G + D ++FV+++ AC H+G V+ G ++F+ M+ +  +EP  
Sbjct: 586 MHGFGKEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWRFFNIMRHECKIEPTV 645

Query: 367 EHYSCLVDLLCRAGELEKAWKLVNDIKSRDYNISLWGALLRACLEQGNIKLGNLAARRAL 426
           EHY+C+VD+L R G+L KA++ + ++     + ++WGALL  C    ++KL    A +  
Sbjct: 646 EHYACIVDMLARTGDLIKAYRFIENMPIPP-DATIWGALLCGCRIHHDVKLAEKVAEKVF 705

Query: 427 ELDPQNAGIFVMLSNLYAGFGLWNEIEHLREFVRKEGLYKGVGCSWIEI 475
           EL+P+N G +V+++N+YA    W +++ LR+ + + GL K  GCSWIEI
Sbjct: 706 ELEPENTGYYVLMANIYAEAEKWEQVKRLRKRIGQRGLRKNPGCSWIEI 744

BLAST of Lsi10G004300 vs. TAIR 10
Match: AT5G04780.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 259.2 bits (661), Expect = 6.4e-69
Identity = 137/401 (34.16%), Postives = 246/401 (61.35%), Query Frame = 0

Query: 74  IFKCIIPRSSLTFNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYALCSSLSACSLIQN 133
           +F  ++ RS +++N +I  ++R+     AL  F  M + G     + + S LSAC +  +
Sbjct: 118 VFDGMLERSLVSWNTMIGLYTRNRMESEALDIFLEMRNEGFKFSEFTISSVLSACGVNCD 177

Query: 134 AVYGKQIHASVGKSGWLCSVFVGSAIVDMYAKNSLINDAVEVFDEIPMKNTVCANALLAG 193
           A+  K++H    K+    +++VG+A++D+YAK  +I DAV+VF+ +  K++V  ++++AG
Sbjct: 178 ALECKKLHCLSVKTCIDLNLYVGTALLDLYAKCGMIKDAVQVFESMQDKSSVTWSSMVAG 237

Query: 194 YGEARMWVEGLELLRKMQALDLKYDKFTLSASLRACAGLSAIALGKQVHAYLLRTVYDIT 253
           Y + + + E L L R+ Q + L+ ++FTLS+ + AC+ L+A+  GKQ+HA + ++ +   
Sbjct: 238 YVQNKNYEEALLLYRRAQRMSLEQNQFTLSSVICACSNLAALIEGKQMHAVICKSGFG-- 297

Query: 254 SDVHLQSTLIEMYGKSGMVEKAWNVFDFAGLGQKEDGKRDIIMWTSMLSVYGRNGCYKRV 313
           S+V + S+ ++MY K G + +++ +F        E  ++++ +W +++S + ++   K V
Sbjct: 298 SNVFVASSAVDMYAKCGSLRESYIIF-------SEVQEKNLELWNTIISGFAKHARPKEV 357

Query: 314 IELYNQMLMEGNKPDRVAFVTVISACGHTGEVNLGIKYFDSMQSDFGLEPGQEHYSCLVD 373
           + L+ +M  +G  P+ V F +++S CGHTG V  G ++F  M++ +GL P   HYSC+VD
Sbjct: 358 MILFEKMQQDGMHPNEVTFSSLLSVCGHTGLVEEGRRFFKLMRTTYGLSPNVVHYSCMVD 417

Query: 374 LLCRAGELEKAWKLVNDIKSRDYNISLWGALLRACLEQGNIKLGNLAARRALELDPQNAG 433
           +L RAG L +A++L+  I   D   S+WG+LL +C    N++L  +AA +  EL+P+NAG
Sbjct: 418 ILGRAGLLSEAYELIKSIPF-DPTASIWGSLLASCRVYKNLELAEVAAEKLFELEPENAG 477

Query: 434 IFVMLSNLYAGFGLWNEIEHLREFVRKEGLYKGVGCSWIEI 475
             V+LSN+YA    W EI   R+ +R   + K  G SWI+I
Sbjct: 478 NHVLLSNIYAANKQWEEIAKSRKLLRDCDVKKVRGKSWIDI 508

BLAST of Lsi10G004300 vs. TAIR 10
Match: AT3G23330.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 249.2 bits (635), Expect = 6.6e-66
Identity = 135/409 (33.01%), Postives = 229/409 (55.99%), Query Frame = 0

Query: 66  FDAQSLNSIFKCIIPRSSLTFNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYALCSSL 125
           F   S+  +F+ +  +  +++N II+ +++ G  + AL     M +  +  D++ L S L
Sbjct: 190 FGIDSVRRVFEVMPRKDVVSYNTIIAGYAQSGMYEDALRMVREMGTTDLKPDSFTLSSVL 249

Query: 126 SACSLIQNAVYGKQIHASVGKSGWLCSVFVGSAIVDMYAKNSLINDAVEVFDEIPMKNTV 185
              S   + + GK+IH  V + G    V++GS++VDMYAK++ I D+  VF  +  ++ +
Sbjct: 250 PIFSEYVDVIKGKEIHGYVIRKGIDSDVYIGSSLVDMYAKSARIEDSERVFSRLYCRDGI 309

Query: 186 CANALLAGYGEARMWVEGLELLRKMQALDLKYDKFTLSASLRACAGLSAIALGKQVHAYL 245
             N+L+AGY +   + E L L R+M    +K      S+ + ACA L+ + LGKQ+H Y+
Sbjct: 310 SWNSLVAGYVQNGRYNEALRLFRQMVTAKVKPGAVAFSSVIPACAHLATLHLGKQLHGYV 369

Query: 246 LRTVYDITSDVHLQSTLIEMYGKSGMVEKAWNVFDFAGLGQKEDGKRDIIMWTSMLSVYG 305
           LR  +   S++ + S L++MY K G ++ A  +FD   +        D + WT+++  + 
Sbjct: 370 LRGGFG--SNIFIASALVDMYSKCGNIKAARKIFDRMNV-------LDEVSWTAIIMGHA 429

Query: 306 RNGCYKRVIELYNQMLMEGNKPDRVAFVTVISACGHTGEVNLGIKYFDSMQSDFGLEPGQ 365
            +G     + L+ +M  +G KP++VAFV V++AC H G V+    YF+SM   +GL    
Sbjct: 430 LHGHGHEAVSLFEEMKRQGVKPNQVAFVAVLTACSHVGLVDEAWGYFNSMTKVYGLNQEL 489

Query: 366 EHYSCLVDLLCRAGELEKAWKLVNDIKSRDYNISLWGALLRACLEQGNIKLGNLAARRAL 425
           EHY+ + DLL RAG+LE+A+  ++ +       S+W  LL +C    N++L    A +  
Sbjct: 490 EHYAAVADLLGRAGKLEEAYNFISKMCVEPTG-SVWSTLLSSCSVHKNLELAEKVAEKIF 549

Query: 426 ELDPQNAGIFVMLSNLYAGFGLWNEIEHLREFVRKEGLYKGVGCSWIEI 475
            +D +N G +V++ N+YA  G W E+  LR  +RK+GL K   CSWIE+
Sbjct: 550 TVDSENMGAYVLMCNMYASNGRWKEMAKLRLRMRKKGLRKKPACSWIEM 588

BLAST of Lsi10G004300 vs. TAIR 10
Match: AT1G50270.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 248.8 bits (634), Expect = 8.6e-66
Identity = 144/442 (32.58%), Postives = 248/442 (56.11%), Query Frame = 0

Query: 34  KIHAHFLRTHFQFVSPNLHSKLILSYSQIIPNFDAQSLNSIFKCIIPRSSLTFNVIISDF 93
           + HAH ++       P + + LI  YS     FD  S   +F     +  +T+  +I  F
Sbjct: 124 QFHAHIVKFGLD-SDPFVRNSLISGYSS-SGLFDFAS--RLFDGAEDKDVVTWTAMIDGF 183

Query: 94  SRHGFPDFALMAFSFMHSNGVPIDTYALCSSLSACSLIQNAVYGKQIHASVGKSGWL-CS 153
            R+G    A++ F  M   GV  +   + S L A   +++  +G+ +H    ++G + C 
Sbjct: 184 VRNGSASEAMVYFVEMKKTGVAANEMTVVSVLKAAGKVEDVRFGRSVHGLYLETGRVKCD 243

Query: 154 VFVGSAIVDMYAKNSLINDAVEVFDEIPMKNTVCANALLAGYGEARMWVEGLELLRKMQA 213
           VF+GS++VDMY K S  +DA +VFDE+P +N V   AL+AGY ++R + +G+ +  +M  
Sbjct: 244 VFIGSSLVDMYGKCSCYDDAQKVFDEMPSRNVVTWTALIAGYVQSRCFDKGMLVFEEMLK 303

Query: 214 LDLKYDKFTLSASLRACAGLSAIALGKQVHAYLLRTVYDITSDVHLQSTLIEMYGKSGMV 273
            D+  ++ TLS+ L ACA + A+  G++VH Y+++   +I +     +TLI++Y K G +
Sbjct: 304 SDVAPNEKTLSSVLSACAHVGALHRGRRVHCYMIKNSIEINTTA--GTTLIDLYVKCGCL 363

Query: 274 EKAWNVFDFAGLGQKEDGKRDIIMWTSMLSVYGRNGCYKRVIELYNQMLMEGNKPDRVAF 333
           E+A  VF+          ++++  WT+M++ +  +G  +   +L+  ML     P+ V F
Sbjct: 364 EEAILVFE-------RLHEKNVYTWTAMINGFAAHGYARDAFDLFYTMLSSHVSPNEVTF 423

Query: 334 VTVISACGHTGEVNLGIKYFDSMQSDFGLEPGQEHYSCLVDLLCRAGELEKAWKLVNDIK 393
           + V+SAC H G V  G + F SM+  F +EP  +HY+C+VDL  R G LE+A  L+  + 
Sbjct: 424 MAVLSACAHGGLVEEGRRLFLSMKGRFNMEPKADHYACMVDLFGRKGLLEEAKALIERMP 483

Query: 394 SRDYNISLWGALLRACLEQGNIKLGNLAARRALELDPQNAGIFVMLSNLYAGFGLWNEIE 453
               N+ +WGAL  +CL   + +LG  AA R ++L P ++G + +L+NLY+    W+E+ 
Sbjct: 484 MEPTNV-VWGALFGSCLLHKDYELGKYAASRVIKLQPSHSGRYTLLANLYSESQNWDEVA 543

Query: 454 HLREFVRKEGLYKGVGCSWIEI 475
            +R+ ++ + + K  G SWIE+
Sbjct: 544 RVRKQMKDQQVVKSPGFSWIEV 551

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SIT72.4e-6833.00Pentatricopeptide repeat-containing protein At2g13600 OS=Arabidopsis thaliana OX... [more]
Q9SN393.1e-6832.52Pentatricopeptide repeat-containing protein DOT4, chloroplastic OS=Arabidopsis t... [more]
Q9LZ199.0e-6834.16Pentatricopeptide repeat-containing protein At5g04780, mitochondrial OS=Arabidop... [more]
Q9LW639.3e-6533.01Putative pentatricopeptide repeat-containing protein At3g23330 OS=Arabidopsis th... [more]
Q9SX451.2e-6432.58Pentatricopeptide repeat-containing protein At1g50270 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
A0A1S3C6K83.1e-25793.35pentatricopeptide repeat-containing protein At2g13600-like OS=Cucumis melo OX=36... [more]
A0A5D3BKX23.1e-25793.35Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A0A0LLD63.4e-25191.42Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G258600 PE=4 SV=1[more]
A0A6J1FYF94.8e-25089.29pentatricopeptide repeat-containing protein At1g50270-like OS=Cucurbita moschata... [more]
A0A6J1CYG81.8e-23383.61pentatricopeptide repeat-containing protein At4g21300-like OS=Momordica charanti... [more]
Match NameE-valueIdentityDescription
XP_038901137.13.9e-26294.33pentatricopeptide repeat-containing protein At5g04780, mitochondrial-like [Benin... [more]
XP_008457622.16.5e-25793.35PREDICTED: pentatricopeptide repeat-containing protein At2g13600-like [Cucumis m... [more]
KAG6571067.11.5e-25089.50Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma sub... [more]
XP_022944055.11.0e-24989.29pentatricopeptide repeat-containing protein At1g50270-like [Cucurbita moschata] ... [more]
XP_023512242.15.0e-24988.87pentatricopeptide repeat-containing protein At2g13600-like [Cucurbita pepo subsp... [more]
Match NameE-valueIdentityDescription
AT2G13600.11.7e-6933.00Pentatricopeptide repeat (PPR) superfamily protein [more]
AT4G18750.12.2e-6932.52Pentatricopeptide repeat (PPR) superfamily protein [more]
AT5G04780.16.4e-6934.16Pentatricopeptide repeat (PPR) superfamily protein [more]
AT3G23330.16.6e-6633.01Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G50270.18.6e-6632.58Pentatricopeptide repeat (PPR) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 158..181
e-value: 0.18
score: 12.1
coord: 85..114
e-value: 0.0071
score: 16.5
coord: 260..280
e-value: 0.021
score: 15.1
coord: 186..212
e-value: 0.0069
score: 16.6
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 293..339
e-value: 1.6E-7
score: 31.4
IPR002885Pentatricopeptide repeatPFAMPF12854PPR_1coord: 360..390
e-value: 2.2E-6
score: 27.3
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 331..363
e-value: 2.1E-4
score: 19.3
coord: 368..396
e-value: 9.7E-6
score: 23.5
coord: 186..218
e-value: 1.8E-4
score: 19.4
coord: 260..280
e-value: 0.0027
score: 15.8
coord: 295..328
e-value: 4.6E-7
score: 27.6
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 82..116
score: 8.769097
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 364..398
score: 9.076014
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 293..327
score: 10.98328
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 258..473
e-value: 7.1E-37
score: 129.4
coord: 82..257
e-value: 1.9E-24
score: 88.6
NoneNo IPR availablePANTHERPTHR47928REPEAT-CONTAINING PROTEIN, PUTATIVE-RELATEDcoord: 29..472
NoneNo IPR availablePANTHERPTHR47928:SF115OS02G0126500 PROTEINcoord: 29..472

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G004300.1Lsi10G004300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0009416 response to light stimulus
biological_process GO:0009451 RNA modification
cellular_component GO:0009507 chloroplast
molecular_function GO:0005515 protein binding