Lsi10G003840 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G003840
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionNADPH-dependent 1-acyldihydroxyacetone phosphate reductase-like
Locationchr10: 5873002 .. 5873841 (+)
RNA-Seq ExpressionLsi10G003840
SyntenyLsi10G003840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACAATACAATCGTAGTTCTAGTCACCGGCTGCGCCAAAGGCGGCATTGGCTACGAATATTGCAAAGCATTTGCCGAAAATGGCTGCATTGTCTTCGCCACCGACCTTCCTCAACGTGCCCACCAACTGTTCGACCAAAGTCCCCACACAGTCCAAACACTTGAACTCGACGTGACATCTGATGAAAGTGTAGAAAAAGCCGTAGAGACCATCATTTCAAAGCATGGGCGCATTGATATCTTGGTAAACAATGCCGGGATCGGTAGCAGCGGCCCGCTCGCCGAGCTTCCATTGCAGTTTGTGAGGAAGGCTTGGGAAGTCAACACACTTGGCCAGCTGAGGATGGTGCAGCAAGTGGTGCCCCATATGGCCTCTCGCCGCAGCGGAGTTATCGTTAACATCGGCAGCGTTGTAGGGAATGTACCGACGCCATGGGCAGGATCGTATTGTGCTAGCAAGGCAGCTGTCCATGCTATGTCGCACGTGCTGCGGCTCGAGCTGAGGCCATTCGGTATCGACGTAGTGATGGTGATGCCTGGGGCTATAAGGTCGAATTTCGGGAGTGCCGCAGTTGAGAATGCGAGAAGTCAAGAATGGAAGCTTTACAAGAAATTTAAAGAAAGCATAGAAGAGAGGGCAAATGCATCACAGAGTGGGAAATCAACTGATGCAGGGGTTTTTGCAAGAGATGTTGTGAAGAAAGTGCTGAGGAAGAAGCCACCAAGGCAAATTGTTTTGGGGCATTTGAGTGGCTTGTTATCCTTGCTTTCTTGGCTTCCACTTTGGGTGAGAGATCTTTACTTCTCCTATCGCTTCAACTTGAATAAAAAGGTATGA

mRNA sequence

ATGGACAATACAATCGTAGTTCTAGTCACCGGCTGCGCCAAAGGCGGCATTGGCTACGAATATTGCAAAGCATTTGCCGAAAATGGCTGCATTGTCTTCGCCACCGACCTTCCTCAACGTGCCCACCAACTGTTCGACCAAAGTCCCCACACAGTCCAAACACTTGAACTCGACGTGACATCTGATGAAAGTGTAGAAAAAGCCGTAGAGACCATCATTTCAAAGCATGGGCGCATTGATATCTTGGTAAACAATGCCGGGATCGGTAGCAGCGGCCCGCTCGCCGAGCTTCCATTGCAGTTTGTGAGGAAGGCTTGGGAAGTCAACACACTTGGCCAGCTGAGGATGGTGCAGCAAGTGGTGCCCCATATGGCCTCTCGCCGCAGCGGAGTTATCGTTAACATCGGCAGCGTTGTAGGGAATGTACCGACGCCATGGGCAGGATCGTATTGTGCTAGCAAGGCAGCTGTCCATGCTATGTCGCACGTGCTGCGGCTCGAGCTGAGGCCATTCGGTATCGACGTAGTGATGGTGATGCCTGGGGCTATAAGGTCGAATTTCGGGAGTGCCGCAGTTGAGAATGCGAGAAGTCAAGAATGGAAGCTTTACAAGAAATTTAAAGAAAGCATAGAAGAGAGGGCAAATGCATCACAGAGTGGGAAATCAACTGATGCAGGGGTTTTTGCAAGAGATGTTGTGAAGAAAGTGCTGAGGAAGAAGCCACCAAGGCAAATTGTTTTGGGGCATTTGAGTGGCTTGTTATCCTTGCTTTCTTGGCTTCCACTTTGGGTGAGAGATCTTTACTTCTCCTATCGCTTCAACTTGAATAAAAAGGTATGA

Coding sequence (CDS)

ATGGACAATACAATCGTAGTTCTAGTCACCGGCTGCGCCAAAGGCGGCATTGGCTACGAATATTGCAAAGCATTTGCCGAAAATGGCTGCATTGTCTTCGCCACCGACCTTCCTCAACGTGCCCACCAACTGTTCGACCAAAGTCCCCACACAGTCCAAACACTTGAACTCGACGTGACATCTGATGAAAGTGTAGAAAAAGCCGTAGAGACCATCATTTCAAAGCATGGGCGCATTGATATCTTGGTAAACAATGCCGGGATCGGTAGCAGCGGCCCGCTCGCCGAGCTTCCATTGCAGTTTGTGAGGAAGGCTTGGGAAGTCAACACACTTGGCCAGCTGAGGATGGTGCAGCAAGTGGTGCCCCATATGGCCTCTCGCCGCAGCGGAGTTATCGTTAACATCGGCAGCGTTGTAGGGAATGTACCGACGCCATGGGCAGGATCGTATTGTGCTAGCAAGGCAGCTGTCCATGCTATGTCGCACGTGCTGCGGCTCGAGCTGAGGCCATTCGGTATCGACGTAGTGATGGTGATGCCTGGGGCTATAAGGTCGAATTTCGGGAGTGCCGCAGTTGAGAATGCGAGAAGTCAAGAATGGAAGCTTTACAAGAAATTTAAAGAAAGCATAGAAGAGAGGGCAAATGCATCACAGAGTGGGAAATCAACTGATGCAGGGGTTTTTGCAAGAGATGTTGTGAAGAAAGTGCTGAGGAAGAAGCCACCAAGGCAAATTGTTTTGGGGCATTTGAGTGGCTTGTTATCCTTGCTTTCTTGGCTTCCACTTTGGGTGAGAGATCTTTACTTCTCCTATCGCTTCAACTTGAATAAAAAGGTATGA

Protein sequence

MDNTIVVLVTGCAKGGIGYEYCKAFAENGCIVFATDLPQRAHQLFDQSPHTVQTLELDVTSDESVEKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQFVRKAWEVNTLGQLRMVQQVVPHMASRRSGVIVNIGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELRPFGIDVVMVMPGAIRSNFGSAAVENARSQEWKLYKKFKESIEERANASQSGKSTDAGVFARDVVKKVLRKKPPRQIVLGHLSGLLSLLSWLPLWVRDLYFSYRFNLNKKV
Homology
BLAST of Lsi10G003840 vs. ExPASy Swiss-Prot
Match: G4N286 (Short-chain dehydrogenase RED1 OS=Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) OX=242507 GN=RED1 PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.1e-30
Identity = 91/277 (32.85%), Postives = 146/277 (52.71%), Query Frame = 0

Query: 8   LVTGCAKGGIGYEYCKAFAENGCIVFATDLPQRAHQLFDQSPHTVQTLELDVTSDESV-- 67
           L+TGC  GGIG+     F +NG IV AT LP  +    + +   +  ++LDVT ++ +  
Sbjct: 10  LITGCGDGGIGHALANNFVQNGFIVIATLLPHESRTHLEHA--KIHVIDLDVTKEDQMIP 69

Query: 68  -EKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQFVRKAWEVNTLGQLRMVQQVVPHM 127
               +E I    G +D+LVNNAGI  +   A+  ++ V K + VN  G +R+V  +   +
Sbjct: 70  FRSTLEEITG--GTLDVLVNNAGICYTMTAADTDVKQVEKMFAVNVFGPMRLVHHLHRML 129

Query: 128 ASRRSGVIVNIGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELRPFGIDVVMVMPGAIR 187
            +   GVIVNIGS+ G  P  +  SY A+KAA+H   + LR+E++PFG+ VV ++ G + 
Sbjct: 130 IAAPRGVIVNIGSIGGVCPYVFGASYNATKAALHHWGNTLRVEMKPFGVHVVNIISGEVA 189

Query: 188 SNFGSAAVENARS-QEWKLYKKFKESIEERANASQSGKSTDAGVFARDVVKKVLRKKPPR 247
           +N   + V + R+  E  +Y    +  ++  N +    S D   +AR VV  V R+  P 
Sbjct: 190 TNILKSDVRDNRTLPEDSVYAPLAQLFKDHVNRTPDAMSPDD--YARGVVAMVQRRSLPA 249

Query: 248 QIVLGHLSGLL-SLLSWLPLWVRDLYFSYRFNLNKKV 280
               G+ +G + +L S+ P  + D  F+  F L   V
Sbjct: 250 WFWHGNATGFIWTLDSFFPRTIWDWLFTRWFKLENLV 280

BLAST of Lsi10G003840 vs. ExPASy Swiss-Prot
Match: A0A140JWS5 (Short-chain dehydrogenase ptmH OS=Penicillium simplicissimum OX=69488 GN=ptmH PE=3 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 9.7e-30
Identity = 97/268 (36.19%), Postives = 142/268 (52.99%), Query Frame = 0

Query: 7   VLVTGCAKGGIGYEYCKAFAENGCIVFAT-DLPQRAHQLFDQSPHTVQTLELDVTSDESV 66
           VL+TGC++GGIG    +AF + G  VFAT    ++   L D    T+  + LDVT +  +
Sbjct: 6   VLITGCSQGGIGSALAEAFHQRGFHVFATARKTEKMTHLRDLDWMTL--IPLDVTQESQI 65

Query: 67  EKAVETIISKH--GRIDILVNNAGIGSSGPLAELPLQFVRKAWEVNTLGQLRMVQQVVPH 126
             AVE +I KH  G +D LVNNAG G   P+ +      R+ +EVN  G LRM+Q+  P 
Sbjct: 66  SAAVE-LIQKHTGGTLDYLVNNAGDGYIIPVLDCDQVHGRQIFEVNFWGPLRMIQEFSPL 125

Query: 127 MASRRSGVIVNIGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELRPFGIDVVMVMPGAI 186
           + + R G IVNI SV       W G Y +SKAA+ A+S  LRLEL+PFG+ V+ VM GA+
Sbjct: 126 LIAAR-GTIVNINSVASETLPLWLGIYSSSKAALLALSETLRLELKPFGVQVLSVMTGAV 185

Query: 187 RSNFGSAAVENARSQEWKLYKKFKESIEERANASQSGKSTDAGVFARDVVKKVLRKKPPR 246
           ++        N R      Y  +++ I  +A  S+      A V+A  VV  +L ++   
Sbjct: 186 QTMIFQT---NYRLPPDSAYVAWEKQIAAQAEGSEQASRMSATVYAERVVGDILNRRGG- 245

Query: 247 QIVLGHLSGLLSLLSW----LPLWVRDL 268
              + +   + S   W    +P ++RDL
Sbjct: 246 ---ITYRGQMASFAYWVVALMPRFLRDL 262

BLAST of Lsi10G003840 vs. ExPASy Swiss-Prot
Match: A0A0E3D8L9 (Short-chain dehydrogenase PC-15 OS=Penicillium crustosum OX=36656 GN=PC-15 PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.3e-29
Identity = 90/237 (37.97%), Postives = 129/237 (54.43%), Query Frame = 0

Query: 7   VLVTGCAKGGIGYEYCKAFAENGCIVFAT-DLPQRAHQLFDQSPHTVQTLELDVTSDESV 66
           VL+TGC++GGIG    + F + G  VFAT    ++   L D    T+  + LDVT +  +
Sbjct: 6   VLITGCSQGGIGSALAEVFHQRGFHVFATARKTEKMKHLRDLDRMTL--IPLDVTQESQI 65

Query: 67  EKAVETIISKH--GRIDILVNNAGIGSSGPLAELPLQFVRKAWEVNTLGQLRMVQQVVPH 126
             AVE +I KH  G +D LVNNAG G   P+ +      R+ +EVN  G LRM+Q+  P 
Sbjct: 66  SAAVE-LIQKHTGGTLDYLVNNAGDGYIIPVLDCDQLHGRQIFEVNFWGPLRMIQEFSPL 125

Query: 127 MASRRSGVIVNIGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELRPFGIDVVMVMPGAI 186
           + + R G IVNI SV       W G Y +SKAA+ A+S  LRLEL+PFG+ V+ VM GA+
Sbjct: 126 LIAAR-GTIVNINSVASETLPLWLGIYSSSKAALLALSETLRLELKPFGVQVLSVMTGAV 185

Query: 187 RSNFGSAAVENARSQEWKLYKKFKESIEERANASQSGKSTDAGVFARDVVKKVLRKK 241
           ++        N R      Y  +++ I  +A  S+      A V+A  VV  +L ++
Sbjct: 186 QTMIFQT---NYRLPPDSAYMTWEKQIAAQAEGSEKASRMSATVYAERVVGDILNRQ 235

BLAST of Lsi10G003840 vs. ExPASy Swiss-Prot
Match: G9N4A9 (Short-chain dehydrogenase virD OS=Hypocrea virens (strain Gv29-8 / FGSC 10586) OX=413071 GN=virD PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 8.2e-29
Identity = 94/278 (33.81%), Postives = 139/278 (50.00%), Query Frame = 0

Query: 8   LVTGCAKGGIGYEYCKAFAENGCIVFATDLPQRAHQLFDQSPHT----VQTLELDVTSDE 67
           LVTGC KGGIG      +   G    AT LP       + S H     +    LDVT ++
Sbjct: 9   LVTGCGKGGIGEALILEYTRRGIYAIATVLPT------ENSDHLTAAGITWFPLDVTDEQ 68

Query: 68  SVEKAVETIIS-KHGRIDILVNNAGIGSSGPLAELPLQFVRKAWEVNTLGQLRMVQQVVP 127
           SV    ++I S   G +D LVNNAGI  +    +  +  V++ ++VN  G +RMV     
Sbjct: 69  SVVDLKKSIASVTDGYLDFLVNNAGICYTMTAIDTDVSAVKRMFDVNVFGPMRMVHH-FH 128

Query: 128 HMASRRSGVIVNIGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELRPFGIDVVMVMPGA 187
            M  + +G IVNIGS+ G VP  +  SY ASKAA+H  S+ LRLE+ PF + V+ V+ G 
Sbjct: 129 DMLIQATGTIVNIGSIGGVVPYMYGASYNASKAALHHYSNTLRLEMSPFNVKVLTVISGE 188

Query: 188 IRSNFGSAAVENARSQEWKLYKKFKESIEERANASQSGKSTDAGVFARDVVKKVLRKKPP 247
           + +N     V +    E   Y     ++E R +  ++ K+T    +A +VV + L+  P 
Sbjct: 189 VGTNILKNDV-HRNLPEGSYYSPL--AVEFRDHVQRTPKTTSRFEYAENVVAQSLKSSPA 248

Query: 248 RQIVLGHLSGL---LSLLSWLPLWVRDLYFSYRFNLNK 278
                G  +G+   L + +W  +W  D +F   FNL K
Sbjct: 249 AWFWTGSATGIIRFLDMFAWRTIW--DFFFYREFNLGK 274

BLAST of Lsi10G003840 vs. ExPASy Swiss-Prot
Match: Q7SHI2 (Short-chain dehydrogenase srdE OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=srdE PE=2 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.8e-28
Identity = 93/278 (33.45%), Postives = 147/278 (52.88%), Query Frame = 0

Query: 3   NTIVVLVTGCAKGGIGYEYCKAFAENGCIVFATDLPQRAHQLFDQSPHTVQTLELDVTSD 62
           +T   L+TGC  GGIG      +   G    AT LP  + +  D++   +   +LDVT++
Sbjct: 5   STKFALITGCGAGGIGEALILEYLRRGIHPIATLLPFESSEHLDKA--GITWFKLDVTNE 64

Query: 63  ESVEKAVETIIS-KHGRIDILVNNAGIGSSGPLAELPLQFVRKAWEVNTLGQLRMVQQVV 122
           ESV +  + +     GR+D LVNNAGI  +    +  ++ V++ ++VN  G +RMV    
Sbjct: 65  ESVVQLKKDVSELTKGRLDFLVNNAGICYTMTAIDTDVKSVQRMFDVNLFGPMRMVHH-F 124

Query: 123 PHMASRRSGVIVNIGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELRPFGIDVVMVMPG 182
             M    SG+IVNIGS+ G VP  +  SY ASKAA+    + LR+EL P G+ V++++ G
Sbjct: 125 HDMLIASSGIIVNIGSIGGVVPFVYGSSYNASKAALAHWGNSLRVELAPLGVRVLVIISG 184

Query: 183 AIRSNFGSAAVENARS-QEWKLYKKFKESIEERANASQSGKSTDAGVFARDVVKKVLRKK 242
            + +N      ++ R+  E   Y    E  +   + +    +TD  V+A++VV + L+K 
Sbjct: 185 EVGTNI--LKNDHGRTLPEGSYYSPMAEEFKNHVHRTPDA-ATDRFVYAKNVVGESLKKS 244

Query: 243 PPRQIVLGHLSGLLSLL-SWLPLWVRDLYFSYRFNLNK 278
           P      G  SG++  L ++ P  V D +FS  FNL K
Sbjct: 245 PTTWFWTGSYSGVIRFLHTFFPKTVFDRWFSSLFNLAK 276

BLAST of Lsi10G003840 vs. ExPASy TrEMBL
Match: A0A0A0LL75 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G248710 PE=3 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 1.4e-140
Identity = 251/279 (89.96%), Postives = 267/279 (95.70%), Query Frame = 0

Query: 1   MDNTIVVLVTGCAKGGIGYEYCKAFAENGCIVFATDLPQRAHQLFDQSPHTVQTLELDVT 60
           MDNTIVVLVTGCAKGGIGYEYCKAFAE+GCIVFATD+P RAHQLF++S  T+QTLELDVT
Sbjct: 1   MDNTIVVLVTGCAKGGIGYEYCKAFAEHGCIVFATDVPHRAHQLFNESFSTIQTLELDVT 60

Query: 61  SDESVEKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQFVRKAWEVNTLGQLRMVQQV 120
           SDESVEKAV+TIISKHGRIDILVNNAGIGSSGPLAELPL+ VRK WEVNTLGQLRMVQQV
Sbjct: 61  SDESVEKAVQTIISKHGRIDILVNNAGIGSSGPLAELPLEAVRKTWEVNTLGQLRMVQQV 120

Query: 121 VPHMASRRSGVIVNIGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELRPFGIDVVMVMP 180
           VPHMASR SGVIVN+GSVVGNVPTPWAGSYCASKA VHAMSHVLRLELRPFGIDVV+VMP
Sbjct: 121 VPHMASRGSGVIVNVGSVVGNVPTPWAGSYCASKATVHAMSHVLRLELRPFGIDVVLVMP 180

Query: 181 GAIRSNFGSAAVENARSQEWKLYKKFKESIEERANASQSGKSTDAGVFARDVVKKVLRKK 240
           GAIRSNFGSA VEN +SQEWKLYKK+K+SIEERANASQSG STDAG+FARDVV KVLRKK
Sbjct: 181 GAIRSNFGSATVENVKSQEWKLYKKYKKSIEERANASQSGNSTDAGIFARDVVNKVLRKK 240

Query: 241 PPRQIVLGHLSGLLSLLSWLPLWVRDLYFSYRFNLNKKV 280
           PP+QIV GHLSGLLS+LSWLPLWVRDLYFSYRFNLN+KV
Sbjct: 241 PPKQIVSGHLSGLLSILSWLPLWVRDLYFSYRFNLNQKV 279

BLAST of Lsi10G003840 vs. ExPASy TrEMBL
Match: A0A5A7TRV0 (NADPH-dependent 1-acyldihydroxyacetone phosphate reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold303G00310 PE=3 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 1.8e-140
Identity = 251/279 (89.96%), Postives = 266/279 (95.34%), Query Frame = 0

Query: 1   MDNTIVVLVTGCAKGGIGYEYCKAFAENGCIVFATDLPQRAHQLFDQSPHTVQTLELDVT 60
           MDNT+VVLVTGCAKGGIGYEYCKAFAE+GCIVFATD+P RAHQL D+S H +QTLELDVT
Sbjct: 1   MDNTVVVLVTGCAKGGIGYEYCKAFAEHGCIVFATDVPHRAHQLLDESFHKIQTLELDVT 60

Query: 61  SDESVEKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQFVRKAWEVNTLGQLRMVQQV 120
           SDESVEKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQ +RKAWEVNTLGQLRMVQQV
Sbjct: 61  SDESVEKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQDIRKAWEVNTLGQLRMVQQV 120

Query: 121 VPHMASRRSGVIVNIGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELRPFGIDVVMVMP 180
           VPHMASRRSGVIVN+GS+VGNVPTPWAGSYCASKAAVHAMSH LRLEL PFGIDVV+VMP
Sbjct: 121 VPHMASRRSGVIVNVGSIVGNVPTPWAGSYCASKAAVHAMSHALRLELMPFGIDVVLVMP 180

Query: 181 GAIRSNFGSAAVENARSQEWKLYKKFKESIEERANASQSGKSTDAGVFARDVVKKVLRKK 240
           GAIRSNFGSA VEN +SQEWKLYKKFKESIEERANASQSGKSTDA VFARDVV KVLRK+
Sbjct: 181 GAIRSNFGSATVENVKSQEWKLYKKFKESIEERANASQSGKSTDAEVFARDVVNKVLRKR 240

Query: 241 PPRQIVLGHLSGLLSLLSWLPLWVRDLYFSYRFNLNKKV 280
           PP+QIV GHLSGLLS+LSWLPLW+RDLYFS RFNLN+KV
Sbjct: 241 PPKQIVSGHLSGLLSILSWLPLWLRDLYFSNRFNLNQKV 279

BLAST of Lsi10G003840 vs. ExPASy TrEMBL
Match: A0A1S4E3R6 (NADPH-dependent 1-acyldihydroxyacetone phosphate reductase-like OS=Cucumis melo OX=3656 GN=LOC103499688 PE=3 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 1.8e-140
Identity = 251/279 (89.96%), Postives = 266/279 (95.34%), Query Frame = 0

Query: 1   MDNTIVVLVTGCAKGGIGYEYCKAFAENGCIVFATDLPQRAHQLFDQSPHTVQTLELDVT 60
           MDNT+VVLVTGCAKGGIGYEYCKAFAE+GCIVFATD+P RAHQL D+S H +QTLELDVT
Sbjct: 1   MDNTVVVLVTGCAKGGIGYEYCKAFAEHGCIVFATDVPHRAHQLLDESFHKIQTLELDVT 60

Query: 61  SDESVEKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQFVRKAWEVNTLGQLRMVQQV 120
           SDESVEKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQ +RKAWEVNTLGQLRMVQQV
Sbjct: 61  SDESVEKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQDIRKAWEVNTLGQLRMVQQV 120

Query: 121 VPHMASRRSGVIVNIGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELRPFGIDVVMVMP 180
           VPHMASRRSGVIVN+GS+VGNVPTPWAGSYCASKAAVHAMSH LRLEL PFGIDVV+VMP
Sbjct: 121 VPHMASRRSGVIVNVGSIVGNVPTPWAGSYCASKAAVHAMSHALRLELMPFGIDVVLVMP 180

Query: 181 GAIRSNFGSAAVENARSQEWKLYKKFKESIEERANASQSGKSTDAGVFARDVVKKVLRKK 240
           GAIRSNFGSA VEN +SQEWKLYKKFKESIEERANASQSGKSTDA VFARDVV KVLRK+
Sbjct: 181 GAIRSNFGSATVENVKSQEWKLYKKFKESIEERANASQSGKSTDAEVFARDVVNKVLRKR 240

Query: 241 PPRQIVLGHLSGLLSLLSWLPLWVRDLYFSYRFNLNKKV 280
           PP+QIV GHLSGLLS+LSWLPLW+RDLYFS RFNLN+KV
Sbjct: 241 PPKQIVSGHLSGLLSILSWLPLWLRDLYFSNRFNLNQKV 279

BLAST of Lsi10G003840 vs. ExPASy TrEMBL
Match: A0A6J1JGF1 (uncharacterized protein LOC111484276 OS=Cucurbita maxima OX=3661 GN=LOC111484276 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 3.5e-139
Identity = 248/279 (88.89%), Postives = 268/279 (96.06%), Query Frame = 0

Query: 1   MDNTIVVLVTGCAKGGIGYEYCKAFAENGCIVFATDLPQRAHQLFDQSPHTVQTLELDVT 60
           MDNTIVVLVTGCAKGGIGYEYCKAF+E GC VFATDLPQRAHQ+FD+SPH V+TLELDVT
Sbjct: 32  MDNTIVVLVTGCAKGGIGYEYCKAFSEQGCHVFATDLPQRAHQVFDESPHRVETLELDVT 91

Query: 61  SDESVEKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQFVRKAWEVNTLGQLRMVQQV 120
           SDESV KAVET+ISK GRIDILVNNAGIGSSGPLAELPLQ V+KAWEVNTLGQLRMVQQV
Sbjct: 92  SDESVAKAVETVISKCGRIDILVNNAGIGSSGPLAELPLQAVKKAWEVNTLGQLRMVQQV 151

Query: 121 VPHMASRRSGVIVNIGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELRPFGIDVVMVMP 180
           VPHMASR SGVIVN+GS+VGNVPTPWAGSYCASKAAVHAMSHVLRLEL+PFGI+VVMV+P
Sbjct: 152 VPHMASRGSGVIVNVGSIVGNVPTPWAGSYCASKAAVHAMSHVLRLELKPFGINVVMVVP 211

Query: 181 GAIRSNFGSAAVENARSQEWKLYKKFKESIEERANASQSGKSTDAGVFARDVVKKVLRKK 240
           GAIRSNFGSA VEN RSQEWKLYKKFKE+IEERANASQSG+S+DAG FARDVVKKVLRK+
Sbjct: 212 GAIRSNFGSATVENVRSQEWKLYKKFKEAIEERANASQSGRSSDAGDFARDVVKKVLRKR 271

Query: 241 PPRQIVLGHLSGLLSLLSWLPLWVRDLYFSYRFNLNKKV 280
           PPR+IVLGHLSGLL+++SWLPLWV+DLYFSY FNLNKKV
Sbjct: 272 PPRRIVLGHLSGLLTIMSWLPLWVQDLYFSYLFNLNKKV 310

BLAST of Lsi10G003840 vs. ExPASy TrEMBL
Match: A0A6J1FXU5 (uncharacterized protein LOC111448738 OS=Cucurbita moschata OX=3662 GN=LOC111448738 PE=3 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.3e-138
Identity = 246/279 (88.17%), Postives = 267/279 (95.70%), Query Frame = 0

Query: 1   MDNTIVVLVTGCAKGGIGYEYCKAFAENGCIVFATDLPQRAHQLFDQSPHTVQTLELDVT 60
           MDN IVVLVTGCAKGGIGYEYCKAF+E GC VFATDLPQRAHQ+FD+SPH V+TLELDVT
Sbjct: 32  MDNRIVVLVTGCAKGGIGYEYCKAFSEQGCHVFATDLPQRAHQVFDESPHRVETLELDVT 91

Query: 61  SDESVEKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQFVRKAWEVNTLGQLRMVQQV 120
           SDESV KAVET+ISK GRIDILVNNAGIGSSGPLAELPLQ VRKAWEVNTLGQLRMVQQV
Sbjct: 92  SDESVAKAVETVISKCGRIDILVNNAGIGSSGPLAELPLQDVRKAWEVNTLGQLRMVQQV 151

Query: 121 VPHMASRRSGVIVNIGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELRPFGIDVVMVMP 180
           VPHMASR SG IVN+GSVVGNVPTPWAGSYCASKAAVHAMSHVLRLEL+PFGIDVVMV+P
Sbjct: 152 VPHMASRGSGAIVNVGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELKPFGIDVVMVVP 211

Query: 181 GAIRSNFGSAAVENARSQEWKLYKKFKESIEERANASQSGKSTDAGVFARDVVKKVLRKK 240
           GAIRSNFGSA VEN RSQEWKLYK+FK++IEERANASQSG+S+DAG FARDVVKKV+RK+
Sbjct: 212 GAIRSNFGSATVENVRSQEWKLYKRFKDAIEERANASQSGRSSDAGAFARDVVKKVMRKR 271

Query: 241 PPRQIVLGHLSGLLSLLSWLPLWVRDLYFSYRFNLNKKV 280
           PPR+IVLGHLSGLL+++SWLPLWV+DLYFS+RFNLNKKV
Sbjct: 272 PPRRIVLGHLSGLLTIMSWLPLWVQDLYFSHRFNLNKKV 310

BLAST of Lsi10G003840 vs. NCBI nr
Match: XP_038902164.1 (short-chain dehydrogenase PC-15-like [Benincasa hispida])

HSP 1 Score: 518.8 bits (1335), Expect = 2.8e-143
Identity = 260/279 (93.19%), Postives = 267/279 (95.70%), Query Frame = 0

Query: 1   MDNTIVVLVTGCAKGGIGYEYCKAFAENGCIVFATDLPQRAHQLFDQSPHTVQTLELDVT 60
           MDN+IVVL+TGCAKGGIGYEYCKAFAE GCIVFATDLPQRAHQL D SPH VQTLELDVT
Sbjct: 1   MDNSIVVLITGCAKGGIGYEYCKAFAEQGCIVFATDLPQRAHQLLDGSPHRVQTLELDVT 60

Query: 61  SDESVEKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQFVRKAWEVNTLGQLRMVQQV 120
           SDESV KAVETIISKHGRIDILVNNAGIGSSGPLAELPLQ VRKAWEVNTLGQLRMVQQV
Sbjct: 61  SDESVAKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQAVRKAWEVNTLGQLRMVQQV 120

Query: 121 VPHMASRRSGVIVNIGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELRPFGIDVVMVMP 180
           VPHMASR SGVIVN+GS+VGNVPTPWAGSYCASKAAVHAMSH LRLELRPFGI+VVMVMP
Sbjct: 121 VPHMASRCSGVIVNVGSIVGNVPTPWAGSYCASKAAVHAMSHALRLELRPFGINVVMVMP 180

Query: 181 GAIRSNFGSAAVENARSQEWKLYKKFKESIEERANASQSGKSTDAGVFARDVVKKVLRKK 240
           GAIRSNFGSA VEN RSQEWKLYKKFKESIEERANASQSGKSTDAGVFAR+VVKKVLRKK
Sbjct: 181 GAIRSNFGSATVENVRSQEWKLYKKFKESIEERANASQSGKSTDAGVFAREVVKKVLRKK 240

Query: 241 PPRQIVLGHLSGLLSLLSWLPLWVRDLYFSYRFNLNKKV 280
           PPRQIVLGHLSG LSLLSWLPLW+RDL FSYRFNLNKKV
Sbjct: 241 PPRQIVLGHLSGWLSLLSWLPLWLRDLCFSYRFNLNKKV 279

BLAST of Lsi10G003840 vs. NCBI nr
Match: XP_004148859.2 (short-chain dehydrogenase ptmH isoform X2 [Cucumis sativus] >KAE8651877.1 hypothetical protein Csa_006073 [Cucumis sativus])

HSP 1 Score: 508.8 bits (1309), Expect = 2.9e-140
Identity = 251/279 (89.96%), Postives = 267/279 (95.70%), Query Frame = 0

Query: 1   MDNTIVVLVTGCAKGGIGYEYCKAFAENGCIVFATDLPQRAHQLFDQSPHTVQTLELDVT 60
           MDNTIVVLVTGCAKGGIGYEYCKAFAE+GCIVFATD+P RAHQLF++S  T+QTLELDVT
Sbjct: 1   MDNTIVVLVTGCAKGGIGYEYCKAFAEHGCIVFATDVPHRAHQLFNESFSTIQTLELDVT 60

Query: 61  SDESVEKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQFVRKAWEVNTLGQLRMVQQV 120
           SDESVEKAV+TIISKHGRIDILVNNAGIGSSGPLAELPL+ VRK WEVNTLGQLRMVQQV
Sbjct: 61  SDESVEKAVQTIISKHGRIDILVNNAGIGSSGPLAELPLEAVRKTWEVNTLGQLRMVQQV 120

Query: 121 VPHMASRRSGVIVNIGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELRPFGIDVVMVMP 180
           VPHMASR SGVIVN+GSVVGNVPTPWAGSYCASKA VHAMSHVLRLELRPFGIDVV+VMP
Sbjct: 121 VPHMASRGSGVIVNVGSVVGNVPTPWAGSYCASKATVHAMSHVLRLELRPFGIDVVLVMP 180

Query: 181 GAIRSNFGSAAVENARSQEWKLYKKFKESIEERANASQSGKSTDAGVFARDVVKKVLRKK 240
           GAIRSNFGSA VEN +SQEWKLYKK+K+SIEERANASQSG STDAG+FARDVV KVLRKK
Sbjct: 181 GAIRSNFGSATVENVKSQEWKLYKKYKKSIEERANASQSGNSTDAGIFARDVVNKVLRKK 240

Query: 241 PPRQIVLGHLSGLLSLLSWLPLWVRDLYFSYRFNLNKKV 280
           PP+QIV GHLSGLLS+LSWLPLWVRDLYFSYRFNLN+KV
Sbjct: 241 PPKQIVSGHLSGLLSILSWLPLWVRDLYFSYRFNLNQKV 279

BLAST of Lsi10G003840 vs. NCBI nr
Match: XP_016902620.1 (PREDICTED: NADPH-dependent 1-acyldihydroxyacetone phosphate reductase-like [Cucumis melo] >KAA0045568.1 NADPH-dependent 1-acyldihydroxyacetone phosphate reductase-like [Cucumis melo var. makuwa] >TYK28826.1 NADPH-dependent 1-acyldihydroxyacetone phosphate reductase-like [Cucumis melo var. makuwa])

HSP 1 Score: 508.4 bits (1308), Expect = 3.8e-140
Identity = 251/279 (89.96%), Postives = 266/279 (95.34%), Query Frame = 0

Query: 1   MDNTIVVLVTGCAKGGIGYEYCKAFAENGCIVFATDLPQRAHQLFDQSPHTVQTLELDVT 60
           MDNT+VVLVTGCAKGGIGYEYCKAFAE+GCIVFATD+P RAHQL D+S H +QTLELDVT
Sbjct: 1   MDNTVVVLVTGCAKGGIGYEYCKAFAEHGCIVFATDVPHRAHQLLDESFHKIQTLELDVT 60

Query: 61  SDESVEKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQFVRKAWEVNTLGQLRMVQQV 120
           SDESVEKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQ +RKAWEVNTLGQLRMVQQV
Sbjct: 61  SDESVEKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQDIRKAWEVNTLGQLRMVQQV 120

Query: 121 VPHMASRRSGVIVNIGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELRPFGIDVVMVMP 180
           VPHMASRRSGVIVN+GS+VGNVPTPWAGSYCASKAAVHAMSH LRLEL PFGIDVV+VMP
Sbjct: 121 VPHMASRRSGVIVNVGSIVGNVPTPWAGSYCASKAAVHAMSHALRLELMPFGIDVVLVMP 180

Query: 181 GAIRSNFGSAAVENARSQEWKLYKKFKESIEERANASQSGKSTDAGVFARDVVKKVLRKK 240
           GAIRSNFGSA VEN +SQEWKLYKKFKESIEERANASQSGKSTDA VFARDVV KVLRK+
Sbjct: 181 GAIRSNFGSATVENVKSQEWKLYKKFKESIEERANASQSGKSTDAEVFARDVVNKVLRKR 240

Query: 241 PPRQIVLGHLSGLLSLLSWLPLWVRDLYFSYRFNLNKKV 280
           PP+QIV GHLSGLLS+LSWLPLW+RDLYFS RFNLN+KV
Sbjct: 241 PPKQIVSGHLSGLLSILSWLPLWLRDLYFSNRFNLNQKV 279

BLAST of Lsi10G003840 vs. NCBI nr
Match: XP_031736558.1 (short-chain dehydrogenase ptmH isoform X1 [Cucumis sativus])

HSP 1 Score: 506.9 bits (1304), Expect = 1.1e-139
Identity = 250/278 (89.93%), Postives = 266/278 (95.68%), Query Frame = 0

Query: 1   MDNTIVVLVTGCAKGGIGYEYCKAFAENGCIVFATDLPQRAHQLFDQSPHTVQTLELDVT 60
           MDNTIVVLVTGCAKGGIGYEYCKAFAE+GCIVFATD+P RAHQLF++S  T+QTLELDVT
Sbjct: 1   MDNTIVVLVTGCAKGGIGYEYCKAFAEHGCIVFATDVPHRAHQLFNESFSTIQTLELDVT 60

Query: 61  SDESVEKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQFVRKAWEVNTLGQLRMVQQV 120
           SDESVEKAV+TIISKHGRIDILVNNAGIGSSGPLAELPL+ VRK WEVNTLGQLRMVQQV
Sbjct: 61  SDESVEKAVQTIISKHGRIDILVNNAGIGSSGPLAELPLEAVRKTWEVNTLGQLRMVQQV 120

Query: 121 VPHMASRRSGVIVNIGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELRPFGIDVVMVMP 180
           VPHMASR SGVIVN+GSVVGNVPTPWAGSYCASKA VHAMSHVLRLELRPFGIDVV+VMP
Sbjct: 121 VPHMASRGSGVIVNVGSVVGNVPTPWAGSYCASKATVHAMSHVLRLELRPFGIDVVLVMP 180

Query: 181 GAIRSNFGSAAVENARSQEWKLYKKFKESIEERANASQSGKSTDAGVFARDVVKKVLRKK 240
           GAIRSNFGSA VEN +SQEWKLYKK+K+SIEERANASQSG STDAG+FARDVV KVLRKK
Sbjct: 181 GAIRSNFGSATVENVKSQEWKLYKKYKKSIEERANASQSGNSTDAGIFARDVVNKVLRKK 240

Query: 241 PPRQIVLGHLSGLLSLLSWLPLWVRDLYFSYRFNLNKK 279
           PP+QIV GHLSGLLS+LSWLPLWVRDLYFSYRFNLN+K
Sbjct: 241 PPKQIVSGHLSGLLSILSWLPLWVRDLYFSYRFNLNQK 278

BLAST of Lsi10G003840 vs. NCBI nr
Match: XP_022986574.1 (uncharacterized protein LOC111484276 [Cucurbita maxima])

HSP 1 Score: 504.2 bits (1297), Expect = 7.1e-139
Identity = 248/279 (88.89%), Postives = 268/279 (96.06%), Query Frame = 0

Query: 1   MDNTIVVLVTGCAKGGIGYEYCKAFAENGCIVFATDLPQRAHQLFDQSPHTVQTLELDVT 60
           MDNTIVVLVTGCAKGGIGYEYCKAF+E GC VFATDLPQRAHQ+FD+SPH V+TLELDVT
Sbjct: 32  MDNTIVVLVTGCAKGGIGYEYCKAFSEQGCHVFATDLPQRAHQVFDESPHRVETLELDVT 91

Query: 61  SDESVEKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQFVRKAWEVNTLGQLRMVQQV 120
           SDESV KAVET+ISK GRIDILVNNAGIGSSGPLAELPLQ V+KAWEVNTLGQLRMVQQV
Sbjct: 92  SDESVAKAVETVISKCGRIDILVNNAGIGSSGPLAELPLQAVKKAWEVNTLGQLRMVQQV 151

Query: 121 VPHMASRRSGVIVNIGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELRPFGIDVVMVMP 180
           VPHMASR SGVIVN+GS+VGNVPTPWAGSYCASKAAVHAMSHVLRLEL+PFGI+VVMV+P
Sbjct: 152 VPHMASRGSGVIVNVGSIVGNVPTPWAGSYCASKAAVHAMSHVLRLELKPFGINVVMVVP 211

Query: 181 GAIRSNFGSAAVENARSQEWKLYKKFKESIEERANASQSGKSTDAGVFARDVVKKVLRKK 240
           GAIRSNFGSA VEN RSQEWKLYKKFKE+IEERANASQSG+S+DAG FARDVVKKVLRK+
Sbjct: 212 GAIRSNFGSATVENVRSQEWKLYKKFKEAIEERANASQSGRSSDAGDFARDVVKKVLRKR 271

Query: 241 PPRQIVLGHLSGLLSLLSWLPLWVRDLYFSYRFNLNKKV 280
           PPR+IVLGHLSGLL+++SWLPLWV+DLYFSY FNLNKKV
Sbjct: 272 PPRRIVLGHLSGLLTIMSWLPLWVQDLYFSYLFNLNKKV 310

BLAST of Lsi10G003840 vs. TAIR 10
Match: AT5G10050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 240.7 bits (613), Expect = 1.4e-63
Identity = 124/268 (46.27%), Postives = 170/268 (63.43%), Query Frame = 0

Query: 2   DNTIVVLVTGCAKGGIGYEYCKAFAENGCIVFATDLPQRAHQLFDQSPHTVQTLELDVTS 61
           D + VVL+TGC++GGIG+   + F E GC V AT   +      +Q    +   ELDV S
Sbjct: 5   DESPVVLITGCSQGGIGHALAREFTEKGCRVVATSRSRSTMTDLEQDSR-LFVKELDVQS 64

Query: 62  DESVEKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQFVRKAWEVNTLGQLRMVQQVV 121
           D++V K +  +I K G+ID+LVNNAG+   GPLAE P+  +   +  N  G +RM Q VV
Sbjct: 65  DQNVSKVLSEVIDKFGKIDVLVNNAGVQCVGPLAETPISAMENTFNTNVFGSMRMTQAVV 124

Query: 122 PHMASRRSGVIVNIGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELRPFGIDVVMVMPG 181
           PHM S++ G IVN+GS+    P PWAG Y A+KAA+HA++  LRLELRPFGIDV+ V+PG
Sbjct: 125 PHMVSKKKGKIVNVGSITVMAPGPWAGVYTATKAAIHALTDTLRLELRPFGIDVINVVPG 184

Query: 182 AIRSNFGSAAVEN-ARSQEWKLYKKFKESIEERANASQSGKSTDAGVFARDVVKKVLRKK 241
            IR+N  ++AV    +  E KLYK ++E+I ERA  SQ    T A  FARD V  VL+K 
Sbjct: 185 GIRTNIANSAVATFNKMPELKLYKPYEEAIRERAFISQRMNPTPAETFARDTVAAVLKKN 244

Query: 242 PPRQIVLGHLSGLLSLLSWLPLWVRDLY 269
           PP     G  S L++++  +PLW++D +
Sbjct: 245 PPAWFSSGRYSTLMAVMYHMPLWLKDFF 271

BLAST of Lsi10G003840 vs. TAIR 10
Match: AT5G65205.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 237.7 bits (605), Expect = 1.2e-62
Identity = 126/274 (45.99%), Postives = 179/274 (65.33%), Query Frame = 0

Query: 2   DNTIVVLVTGCAKGGIGYEYCKAFAENGCIVFATDLPQRA-HQLFDQSPHTVQTLELDVT 61
           D T VVL+TGC++GGIG+   + F+ NGC V AT   Q+   +L   S   VQ  ELDV 
Sbjct: 6   DETPVVLITGCSQGGIGHALAREFSANGCRVVATSRSQKTMTELEKDSKFFVQ--ELDVQ 65

Query: 62  SDESVEKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQFVRKAWEVNTLGQLRMVQQV 121
           S++SV K V  +I K G+ID+LVNNAG+   GPLAE+P+  +   +  N LG +RM Q V
Sbjct: 66  SEQSVSKVVSKVIDKFGQIDVLVNNAGVQCIGPLAEIPISAMDYTFNTNVLGSMRMTQAV 125

Query: 122 VPHMASRRSGVIVNIGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELRPFGIDVVMVMP 181
           VPHMAS++ G IVNIGS+    P PWAG Y ASKAA+HA++  LRLEL+PFGIDV+ ++P
Sbjct: 126 VPHMASKKKGKIVNIGSISIMAPGPWAGVYTASKAALHALTDTLRLELKPFGIDVINIVP 185

Query: 182 GAIRSNFGSAAVENARS-QEWKLYKKFKESIEERANASQSGKSTDAGVFARDVVKKVLRK 241
           G I+SN  ++ + +  +  E KLYK F+++I ERA  SQ+ K      FA++ V  VL+K
Sbjct: 186 GGIQSNIANSGISSFNNLPELKLYKPFEDAIRERAFLSQNIKPIPTETFAKETVSVVLKK 245

Query: 242 KPPRQIVLGHLSGLLSLLSWLPLWVRDLYFSYRF 274
            PP     G LS +++++  +P++++D   +  F
Sbjct: 246 NPPAWYSTGRLSTVMAIMHHIPIFMKDFLLTKSF 277

BLAST of Lsi10G003840 vs. TAIR 10
Match: AT1G10310.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 79.7 bits (195), Expect = 4.1e-15
Identity = 60/196 (30.61%), Postives = 92/196 (46.94%), Query Frame = 0

Query: 7   VLVTGCAKG---GIGYEYCK-AFAENGCIVFATDLPQRAHQLFDQSPHTVQTLELDVTSD 66
           VL+TG +KG    +  E  K      GC      L     +L   + H +  L  DV S+
Sbjct: 20  VLITGVSKGLGRALALELAKRGHTVIGCARSQEKLTALQSELSSSTNHLL--LTADVKSN 79

Query: 67  ESVEKAVETIISKHGRIDILVNNAG-IGSSGPLAELPLQFVRKAWEVNTLGQLRMVQQVV 126
            SVE+   TI+ K G  DI+VNNAG I  +  + E+  +      + N  G   +++  +
Sbjct: 80  SSVEEMAHTIVEKKGVPDIIVNNAGTINKNSKIWEVSAEDFDNVMDTNVKGVANVLRHFI 139

Query: 127 PHMASRRSGVIVNIGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELRPFGIDVVMVMPG 186
           P M  R+ G+IVN+ S  G         YCASK A+  +S  +  E+   G+ VV + PG
Sbjct: 140 PLMLPRKQGIIVNMSSGWGRSGAALVAPYCASKWAIEGLSRAVAKEVVE-GMAVVALNPG 199

Query: 187 AIRSNFGSAAVENARS 198
            I +   ++   N+ S
Sbjct: 200 VINTELLTSCFGNSAS 212

BLAST of Lsi10G003840 vs. TAIR 10
Match: AT3G26760.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 75.1 bits (183), Expect = 1.0e-13
Identity = 50/182 (27.47%), Postives = 86/182 (47.25%), Query Frame = 0

Query: 6   VVLVTGCAKGGIGYEYCKAFAENGCIVFATDLPQRA-HQLFDQSPHTVQTLELDVTSDES 65
           V ++TG A  GIG    + F   G  V   D+ + A H +  +       L  DVT +E 
Sbjct: 40  VAVITGGA-SGIGKATAEEFVSQGAQVIIVDIDEEAGHMVATELGSAAHFLRCDVTEEEQ 99

Query: 66  VEKAVETIISKHGRIDILVNNAGIG---SSGPLAELPLQFVRKAWEVNTLGQLRMVQQVV 125
           + KAVET +++HG++D+++N+AGI    S   +A+L +    K   +N  G +  ++   
Sbjct: 100 IAKAVETAVTRHGKLDVMLNSAGISCSISPPSIADLDMDTYDKVMRLNVRGTVLGIKHAA 159

Query: 126 PHMASRRSGVIVNIGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELRPFGIDVVMVMPG 184
             M    SG I+ + S+ G +      +Y  SK  +  +   +  EL   G+ +  + P 
Sbjct: 160 RAMIPAGSGSILCLSSISGLMGGLGPHAYSISKFTIPGVVKTVASELCKHGLRINCISPA 219

BLAST of Lsi10G003840 vs. TAIR 10
Match: AT1G24360.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 70.1 bits (170), Expect = 3.2e-12
Identity = 56/187 (29.95%), Postives = 86/187 (45.99%), Query Frame = 0

Query: 6   VVLVTGCAKGGIGYEYCKAFAENGCIVF------ATDLPQRAHQLFDQSPHTVQTLELDV 65
           VV++TG ++ GIG     A  + GC V       A +  + A Q+ +     + T   DV
Sbjct: 78  VVVITGASR-GIGKAIALALGKAGCKVLVNYARSAKEAEEVAKQIEEYGGQAI-TFGGDV 137

Query: 66  TSDESVEKAVETIISKHGRIDILVNNAGIGSSGPLAELPLQFVRKAWEVNTLGQLRMVQQ 125
           +    V+  ++T + K G ID++VNNAGI     L  +      +   +N  G     Q 
Sbjct: 138 SKATDVDAMMKTALDKWGTIDVVVNNAGITRDTLLIRMKQSQWDEVIALNLTGVFLCTQA 197

Query: 126 VVPHMASRRSGVIVNIGSVVGNVPTPWAGSYCASKAAVHAMSHVLRLELRPFGIDVVMVM 185
            V  M  ++ G I+NI SVVG +      +Y A+K  V + S     E     I+V +V 
Sbjct: 198 AVKIMMKKKRGRIINISSVVGLIGNIGQANYAAAKGGVISFSKTAAREGASRNINVNVVC 257

Query: 186 PGAIRSN 187
           PG I S+
Sbjct: 258 PGFIASD 262

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
G4N2861.1e-3032.85Short-chain dehydrogenase RED1 OS=Magnaporthe oryzae (strain 70-15 / ATCC MYA-46... [more]
A0A140JWS59.7e-3036.19Short-chain dehydrogenase ptmH OS=Penicillium simplicissimum OX=69488 GN=ptmH PE... [more]
A0A0E3D8L91.3e-2937.97Short-chain dehydrogenase PC-15 OS=Penicillium crustosum OX=36656 GN=PC-15 PE=3 ... [more]
G9N4A98.2e-2933.81Short-chain dehydrogenase virD OS=Hypocrea virens (strain Gv29-8 / FGSC 10586) O... [more]
Q7SHI21.8e-2833.45Short-chain dehydrogenase srdE OS=Neurospora crassa (strain ATCC 24698 / 74-OR23... [more]
Match NameE-valueIdentityDescription
A0A0A0LL751.4e-14089.96Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G248710 PE=3 SV=1[more]
A0A5A7TRV01.8e-14089.96NADPH-dependent 1-acyldihydroxyacetone phosphate reductase-like OS=Cucumis melo ... [more]
A0A1S4E3R61.8e-14089.96NADPH-dependent 1-acyldihydroxyacetone phosphate reductase-like OS=Cucumis melo ... [more]
A0A6J1JGF13.5e-13988.89uncharacterized protein LOC111484276 OS=Cucurbita maxima OX=3661 GN=LOC111484276... [more]
A0A6J1FXU51.3e-13888.17uncharacterized protein LOC111448738 OS=Cucurbita moschata OX=3662 GN=LOC1114487... [more]
Match NameE-valueIdentityDescription
XP_038902164.12.8e-14393.19short-chain dehydrogenase PC-15-like [Benincasa hispida][more]
XP_004148859.22.9e-14089.96short-chain dehydrogenase ptmH isoform X2 [Cucumis sativus] >KAE8651877.1 hypoth... [more]
XP_016902620.13.8e-14089.96PREDICTED: NADPH-dependent 1-acyldihydroxyacetone phosphate reductase-like [Cucu... [more]
XP_031736558.11.1e-13989.93short-chain dehydrogenase ptmH isoform X1 [Cucumis sativus][more]
XP_022986574.17.1e-13988.89uncharacterized protein LOC111484276 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G10050.11.4e-6346.27NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G65205.11.2e-6245.99NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G10310.14.1e-1530.61NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G26760.11.0e-1327.47NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G24360.13.2e-1229.95NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 171..188
score: 30.77
coord: 150..169
score: 35.38
coord: 124..140
score: 35.71
coord: 77..88
score: 66.54
coord: 7..24
score: 24.56
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 77..88
score: 63.14
coord: 130..138
score: 62.38
coord: 150..169
score: 36.32
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 6..191
e-value: 1.4E-50
score: 171.4
NoneNo IPR availableGENE3D3.40.50.720coord: 5..253
e-value: 3.7E-65
score: 221.9
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 8..238
e-value: 1.6E-40
score: 136.8
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 8..186
e-value: 3.5E-4
score: 17.6
NoneNo IPR availablePANTHERPTHR44169NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASEcoord: 3..278
NoneNo IPR availablePANTHERPTHR44169:SF5NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEINcoord: 3..278
NoneNo IPR availableCDDcd0537417beta-HSD-like_SDR_ccoord: 6..246
e-value: 2.05684E-86
score: 256.389
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 137..165
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 6..250

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G003840.1Lsi10G003840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0016491 oxidoreductase activity