Lsi10G003700 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G003700
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionRNase H domain-containing protein
Locationchr10: 5711594 .. 5712564 (+)
RNA-Seq ExpressionLsi10G003700
SyntenyLsi10G003700
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTAAAGTCAATTTAAGTATAACTTAATAAACAAGACAGTAAATACGATTCTTAAGATCAAATATTTGTTTTTCATTCTACAATTAGTTCAACTCAAAGAAAAAAGAAGTTTATGTAATTGAGTTAAGAAATTGAGTGGACATTTTTATTCTCTGATCGTAGTCTAATAAAACCCACAAAATAAAGTAGGGAGAAAATAAGGAATCTCAAATTAAATTACTCTGGCAATACAAGTAACACATAAATTTCATAAACCAAATACATGGTTGCTATGATTTATTATAAAGACTATAAGTAAATTAGAAAGAAGTTTGGGCTCCCCTTGTGCCGATTGAGTGTAAGGTTAAATGGATCTCGAGGTACATTGATGATATTGTTGTTGTTCCTAGGCTATCAGGTGTGGAGACTTGTGGTGTTCGTGGAAAGGTTGTGTCTAGTCATCGAAGGGGGGAGTGAGTGTTCATTAGGGTGTGCACTGGTGTTTGCCACCACGTGGAGCTCTGAAATTAAATATCGACATTGCTTGTCATCCGGTGAACTCAAAGATGGGGATGGGAGCTGTTGTGCGAGATGATTGTAGGAGAGTCATTACTGCCCATGCTCTTGTTCGTCCAGGTTGTGTATCAGCCAAAGATGGTGAAATTTTTGCTTTTATAGCTGGTTTGAAGTTAGCTGAAGTTCACAGATGCTTAGGGTGTTGGGTGAAGATGGATGCTGAAATTGTGGTGAAGAAGATACAAGTTCTTTCCTTTTCATTAATTCCTAATGGTATATATTATTTATGTTGAAGCATTCTCTTTAATGCAAAGGTTGGGGTTTATGCTCATAGAAGGTGTAATAAGGTGACCCATAATTTGGTCACTCAAGTTATTGTAGCCAATTCTTCTTAGGTTTAGATTGACTGTATTCTCCCTTGGTTCTCAATCATTGTCGGGGCTGAGATGGGGGACTTGGATCTTATTGGATGA

mRNA sequence

ATGATTAAAAAAGAAGTTTGGGCTCCCCTTGTGCCGATTGAGTGTAAGGTTAAATGGATCTCGAGGTACATTGATGATATTGTTGTTGTTCCTAGGCTATCAGGTGTGGAGACTTGTGGTGTTCGTGGAAAGGGTGTGCACTGGTGTTTGCCACCACGTGGAGCTCTGAAATTAAATATCGACATTGCTTGTCATCCGGTGAACTCAAAGATGGGGATGGGAGCTGTTGTGCGAGATGATTGTAGGAGAGTCATTACTGCCCATGCTCTTGTTCGTCCAGGTTGTGTATCAGCCAAAGATGGTGAAATTTTTGCTTTTATAGCTGGTTTGAAGTTAGCTGAAGTTCACAGATGCTTAGGGTGTTGGGTGAAGATGGATGCTGAAATTGTGGTGAAGAAGATACAAATTGACTGTATTCTCCCTTGGTTCTCAATCATTGTCGGGGCTGAGATGGGGGACTTGGATCTTATTGGATGA

Coding sequence (CDS)

ATGATTAAAAAAGAAGTTTGGGCTCCCCTTGTGCCGATTGAGTGTAAGGTTAAATGGATCTCGAGGTACATTGATGATATTGTTGTTGTTCCTAGGCTATCAGGTGTGGAGACTTGTGGTGTTCGTGGAAAGGGTGTGCACTGGTGTTTGCCACCACGTGGAGCTCTGAAATTAAATATCGACATTGCTTGTCATCCGGTGAACTCAAAGATGGGGATGGGAGCTGTTGTGCGAGATGATTGTAGGAGAGTCATTACTGCCCATGCTCTTGTTCGTCCAGGTTGTGTATCAGCCAAAGATGGTGAAATTTTTGCTTTTATAGCTGGTTTGAAGTTAGCTGAAGTTCACAGATGCTTAGGGTGTTGGGTGAAGATGGATGCTGAAATTGTGGTGAAGAAGATACAAATTGACTGTATTCTCCCTTGGTTCTCAATCATTGTCGGGGCTGAGATGGGGGACTTGGATCTTATTGGATGA

Protein sequence

MIKKEVWAPLVPIECKVKWISRYIDDIVVVPRLSGVETCGVRGKGVHWCLPPRGALKLNIDIACHPVNSKMGMGAVVRDDCRRVITAHALVRPGCVSAKDGEIFAFIAGLKLAEVHRCLGCWVKMDAEIVVKKIQIDCILPWFSIIVGAEMGDLDLIG
Homology
BLAST of Lsi10G003700 vs. ExPASy TrEMBL
Match: M5WZF6 (RNase H domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa020664mg PE=4 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 3.6e-08
Identity = 38/101 (37.62%), Postives = 51/101 (50.50%), Query Frame = 0

Query: 44  KGVHWCLPPRGALKLNIDIACHPVNSKMGMGAVVRDDCRRVITAHALVRPGCVSAKDGEI 103
           K   W  PP G  KLN+D A  P     G+GAVVR+D   V+ A AL      S+K  EI
Sbjct: 31  KSTKWSPPPAGKYKLNVDAAFIPETGVGGIGAVVRNDKGEVMAAMALPLASATSSKHAEI 90

Query: 104 FAFIAGLKLAEVHRCLGCWVKMDAEIVVKKIQIDCILPWFS 145
            AF+ G+K A    C    ++ D++ VV  ++ D    W S
Sbjct: 91  MAFLFGMKFARDAGCSSILIESDSQGVVNDVKKDEEESWAS 131

BLAST of Lsi10G003700 vs. ExPASy TrEMBL
Match: A0A5D3DDI5 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G001030 PE=4 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 5.2e-07
Identity = 32/93 (34.41%), Postives = 52/93 (55.91%), Query Frame = 0

Query: 40  GVRGKGVHWCLPPRGALKLNIDIACHPVNSKMGMGAVVRDDCRRVITAHALVRPGCVSAK 99
           G R +   W       LKLN+ +ACH ++ K G+G V+R+D RR++T  +L + G ++ +
Sbjct: 73  GHRSRLQQWSPSVVSRLKLNVVVACHLLHDKFGVGIVIRNDKRRLVTVTSLCKVGSLNVE 132

Query: 100 DGEIFAFIAGLKLAEVHRCLGCWVKMDAEIVVK 133
            G + A + GL+       L   + MD E+VVK
Sbjct: 133 VGGVMALLKGLRFIGDGGFLNNVIGMDVEVVVK 165

BLAST of Lsi10G003700 vs. ExPASy TrEMBL
Match: A0A803PBY3 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.6e-06
Identity = 32/93 (34.41%), Postives = 48/93 (51.61%), Query Frame = 0

Query: 44  KGVH-WCLPPRGALKLNIDIACHPVNSKMGMGAVVRDDCRRVITAHALVRPGCVSAKDGE 103
           KG++ W  PP G   +N D A    +   G+ AV+RD+C R++ A A  + GCVS    E
Sbjct: 785 KGINGWLAPPTGTYMMNYDAALCQGHQGSGVAAVIRDNCGRLVAAEACFQAGCVSILLAE 844

Query: 104 IFAFIAGLKLAEVHRCLGCWVKMDAEIVVKKIQ 136
           + A I GL L +       +V  D    +K++Q
Sbjct: 845 VLAIILGLNLVQRLEARPFFVSSDNLTAIKQLQ 877

BLAST of Lsi10G003700 vs. ExPASy TrEMBL
Match: A0A5A7TDE1 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00810 PE=4 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 3.4e-06
Identity = 31/93 (33.33%), Postives = 51/93 (54.84%), Query Frame = 0

Query: 40  GVRGKGVHWCLPPRGALKLNIDIACHPVNSKMGMGAVVRDDCRRVITAHALVRPGCVSAK 99
           G R +   W       LKLN+ +ACH ++ K G+G V+R+D RR++T  +L + G ++ +
Sbjct: 24  GHRSRLQQWSPSVVSRLKLNVVVACHLLHDKFGVGIVIRNDKRRLVTVTSLCKVGSLNVE 83

Query: 100 DGEIFAFIAGLKLAEVHRCLGCWVKMDAEIVVK 133
            G + A +  L+       L   + MD E+VVK
Sbjct: 84  VGGVMALLKELRFIGEGGFLNNVIGMDVEVVVK 116

BLAST of Lsi10G003700 vs. ExPASy TrEMBL
Match: A0A6J5XRX0 (RNase H domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS37308 PE=4 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 2.8e-05
Identity = 30/67 (44.78%), Postives = 39/67 (58.21%), Query Frame = 0

Query: 47  HWCLPPRGALKLNIDIACHPVNSKMGMGAVVRDDCRRVITAHALVRPGCVSAKDGEIFAF 106
           HWC PP G LKLN+D AC     K G+G VVR+    +++A  +   GC SAK  E+ A 
Sbjct: 40  HWCPPPPGKLKLNVDGACSTSVWKCGLGTVVRNVHGDLLSAILVPITGCFSAKITELLAI 99

Query: 107 IAGLKLA 114
             GL+ A
Sbjct: 100 WEGLQFA 106

BLAST of Lsi10G003700 vs. NCBI nr
Match: XP_031091124.1 (uncharacterized protein LOC115996122 [Ipomoea triloba])

HSP 1 Score: 69.3 bits (168), Expect = 3.3e-08
Identity = 38/117 (32.48%), Postives = 58/117 (49.57%), Query Frame = 0

Query: 31  PRLSGVETCGVRGKGVHWCLPPRGALKLNIDIACHPVNSKMGMGAVVRDDCRRVITAHAL 90
           PR +G+ T        HWC P RG LKLN D+A H  N+ MG+G V+RDD  R + +  +
Sbjct: 205 PRYTGINTV-----ETHWCRPERGRLKLNTDVALHHENNLMGLGWVLRDDDGRFLASKNV 264

Query: 91  VRPGCVSAKDGEIFAFIAGLKLAEVHRCLGCWVKMDAEIVVKKIQIDCILPWFSIIV 148
             PG  +  + E F+    L   +        V+MD++IV   +     +  F ++V
Sbjct: 265 CMPGRFTVTEAEAFSLREALSWLKDSGMGSVDVEMDSQIVYNALHKPSFISAFGMLV 316

BLAST of Lsi10G003700 vs. NCBI nr
Match: TYK21731.1 (hypothetical protein E5676_scaffold859G001030 [Cucumis melo var. makuwa])

HSP 1 Score: 64.3 bits (155), Expect = 1.1e-06
Identity = 32/93 (34.41%), Postives = 52/93 (55.91%), Query Frame = 0

Query: 40  GVRGKGVHWCLPPRGALKLNIDIACHPVNSKMGMGAVVRDDCRRVITAHALVRPGCVSAK 99
           G R +   W       LKLN+ +ACH ++ K G+G V+R+D RR++T  +L + G ++ +
Sbjct: 73  GHRSRLQQWSPSVVSRLKLNVVVACHLLHDKFGVGIVIRNDKRRLVTVTSLCKVGSLNVE 132

Query: 100 DGEIFAFIAGLKLAEVHRCLGCWVKMDAEIVVK 133
            G + A + GL+       L   + MD E+VVK
Sbjct: 133 VGGVMALLKGLRFIGDGGFLNNVIGMDVEVVVK 165

BLAST of Lsi10G003700 vs. NCBI nr
Match: XP_008229542.1 (PREDICTED: uncharacterized protein LOC103328904 [Prunus mume])

HSP 1 Score: 62.8 bits (151), Expect = 3.1e-06
Identity = 42/121 (34.71%), Postives = 56/121 (46.28%), Query Frame = 0

Query: 22  RYIDDIVVVPRLSGVETCGVRGKGV--------HWCLPPRGALKLNIDIACHPVNSKMGM 81
           R+ DD+        VE   V    V        HWC PP G LKLN+D AC     K G+
Sbjct: 7   RHPDDLYATAHRLSVEFISVNTAPVHTHSPSSTHWCPPPPGKLKLNVDGACSTFVWKCGL 66

Query: 82  GAVVRDDCRRVITAHALVRPGCVSAKDGEIFAFIAGLKLAEVHRCLGCWVKMDAEIVVKK 135
           GAVVR+    ++ A ++   GC SAK  E+ A   GL+ A         V+ DA+  +  
Sbjct: 67  GAVVRNVHGDLLGAISVPITGCFSAKITELLAIRKGLQFAWEANYDSLVVETDAKNAIND 126

BLAST of Lsi10G003700 vs. NCBI nr
Match: XP_019163573.1 (PREDICTED: uncharacterized protein LOC109159918 [Ipomoea nil])

HSP 1 Score: 62.4 bits (150), Expect = 4.1e-06
Identity = 33/84 (39.29%), Postives = 47/84 (55.95%), Query Frame = 0

Query: 48  WCLPPRGALKLNIDIACHPVNSKMGMGAVVRDDCRRVITAHALVRPGCVSAKDGEIFAFI 107
           W  PP G LKLNID + H  +S +GMG ++RD     + A + VRPG +S ++ E  A  
Sbjct: 530 WAAPPNGKLKLNIDASIHHNSSHIGMGWIIRDHHGFFVAAISDVRPGPLSPREAEALAVR 589

Query: 108 AGLKLAEVHRCLGCWVKMDAEIVV 132
             L   + H   G  V+ DAEI++
Sbjct: 590 EALSWLKRHHWDGLVVETDAEILL 613

BLAST of Lsi10G003700 vs. NCBI nr
Match: KAA0040106.1 (hypothetical protein E6C27_scaffold366G00810 [Cucumis melo var. makuwa])

HSP 1 Score: 61.6 bits (148), Expect = 6.9e-06
Identity = 31/93 (33.33%), Postives = 51/93 (54.84%), Query Frame = 0

Query: 40  GVRGKGVHWCLPPRGALKLNIDIACHPVNSKMGMGAVVRDDCRRVITAHALVRPGCVSAK 99
           G R +   W       LKLN+ +ACH ++ K G+G V+R+D RR++T  +L + G ++ +
Sbjct: 24  GHRSRLQQWSPSVVSRLKLNVVVACHLLHDKFGVGIVIRNDKRRLVTVTSLCKVGSLNVE 83

Query: 100 DGEIFAFIAGLKLAEVHRCLGCWVKMDAEIVVK 133
            G + A +  L+       L   + MD E+VVK
Sbjct: 84  VGGVMALLKELRFIGEGGFLNNVIGMDVEVVVK 116

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
M5WZF63.6e-0837.62RNase H domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_... [more]
A0A5D3DDI55.2e-0734.41Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A803PBY32.6e-0634.41Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A5A7TDE13.4e-0633.33Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A6J5XRX02.8e-0544.78RNase H domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCU... [more]
Match NameE-valueIdentityDescription
XP_031091124.13.3e-0832.48uncharacterized protein LOC115996122 [Ipomoea triloba][more]
TYK21731.11.1e-0634.41hypothetical protein E5676_scaffold859G001030 [Cucumis melo var. makuwa][more]
XP_008229542.13.1e-0634.71PREDICTED: uncharacterized protein LOC103328904 [Prunus mume][more]
XP_019163573.14.1e-0639.29PREDICTED: uncharacterized protein LOC109159918 [Ipomoea nil][more]
KAA0040106.16.9e-0633.33hypothetical protein E6C27_scaffold366G00810 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 54..144
e-value: 1.3E-5
score: 27.2
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 61..135
e-value: 3.5E-6
score: 26.8
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 58..147
e-value: 1.37727E-9
score: 51.1608
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 56..142

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G003700.1Lsi10G003700.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity