Lsi10G003400 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G003400
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionRING/U-box superfamily protein
Locationchr10: 5354460 .. 5359728 (+)
RNA-Seq ExpressionLsi10G003400
SyntenyLsi10G003400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCAGATATTCCATTGCACCAAAACGGCGTCGTTTTATGGAAGGATTAATACGACGTCGTATAACCTTTGCTCTCTTCTCTTTTTATCTTCTCTCTCACAAATTTTGCTCTCTTTTTCTCTTTTTATTTATTTATATTTATTTTATATTTATATTTTACTTATTTCATCGGAAGAACTCCCACCATTTAGTCGTTGTCCTTTCACTCTCTTCGCCGGATTTTCCAACGATCTTCCCGGAGGGAAACGTTCTCCGGCGTTTCCCGATTTCTCCGGATCCGATTTATTCATCGAATCTCGTTATCGATTGTTTCGTTTTGCTTTTCTTTTTCCCCTTTTTTGTATTACCCACTCAGCTTTGCCTTTCTTCTTTCAATCTGACTTCAATTTCTTCTTCTCTTTCCCCAATTCCCTTTCCCTATTTCTCAATGGACGACTGTATCAGCAGCGATGAGGACTACTATGACTCCGATCGAGATTCTCTACATGGTCTTGAGAACGAGGACTCTGAAATTCAGTGGGTTCCTAAAGGTCCCGCGACTAAGGTATTCTATTTCTTCCCAAATTCTAATTGCCTTTAATTACTTGATTTCTAGGGATTGCAAGTTCTAACTCTTGTTTATGGATTCAATTTTAGAGAGTTTCTTTGTTTCTTTTTGTTTTCTGATGGCAGGGAATGAATTCATTGTATTGAAATATAACGTTCTTTGTTTTTCCTTTTGGATTCTTATTGTTTTTGTTGTTATTATTACTAAAGAAAAAAAAGGAGAGGTTTTTCTCCAAGTTTGTGATGATGGGGCGGCGGAATTTGGTGATATAATGCTTGTAATTTCTTTCCTGTTGAGAATTACGGGAATTTTCTAATCGATCGTGTTTATGGAGACCCTTTTTGTTGTTTTCAGGTTATTACGAAAGAGTCTCTCCTTGCGGCACAGGTTTAGCACTGCTTCCTTCTTTCTTATAAACGATCTTTTGTCTGATTTTATCGTTTAGTATGTACCTGGATTCTTTTTTGTTGTTTGAATTTGATTTTAGGGTTGTTTTGTTTTCATGTAGAAGGAGGATATGCGTAGAGTAATGGACTTGCTATCGTTGAGGGAGTACCATGCTCGGACCTTACTCATTCATTACCGATGGGATGTAGAGAAATTGTTTGCAGTACTTGTGGAGAAAGGGAAAAGTCATTTGTTTGCTACAGCTGGTGTTACTATGATTGAGAATCGAAGTAATAGTTCCTCTGAGCCTTCTTCCATGGTGATGTGTGATATCTGCATGGAGGAAGTACATGACTACGATGCAACAAGAGTGGATTGTGGCCATTGCTTTTGTAATAACTGTAAGCATTTCCCCTCCTCTAAAATTAGATTGTCTTTTTTTAAAAGAATTTGTTCCCTTATTCTTTTTACATGCACAAACAGTGCAGTCTACTTTTGGCCTTGAGAACTGATATTGTAAAGATTGGTTGTGATGTTAGTTGTTAGCAATTGTTGTGGAATGAAATTGTGCTGAGGTCATATAAATTACCAATAATTGGCTGAGCTTTATTGTTCCTTATTTCGTTGAAAACTTGAAACAATTATCTGAGATCAAATTCACTTTATGTCTTGCTTCAGGTTGGACTGAACATTTCATTGTGAAAATAAATGAGGGTCAAAGTCGGAGAATCAGGTGCATGGCCCACAAATGTAATGCGATTTGTGATGAAGCTGTCGTTAGGAATCTAGTTAGTAAAAGGCATCCAGATCTTGCTAATAAATTTGATCGTTTTCTTCTTGAATCATACATTGAGGATAACAAAAGAGTCAAGTGGTGTCCAAGTACTCCTCATTGTGGCAATGCTATACGCGTGGAGGATGATGAGTTTTGTGAAGTAGAATGTTCGTGTGGTATTCAGTTTTGTTTCAGTTGCTTATCAGAAGCTCACTCACCGTGTTCATGTTTGATGTGGGAACTTTGGATTAAGAAGTGCCGAGATGAATCTGAAACTGTTAACTGGATTACAGTACATACAAAGCCCTGCCCAAAGTGCCATAAACCTGTCGAGAAGAATGGAGGTTGTAACCTTGTCAGCTGCATCTGTGGTCAGGCATTTTGGTAGGATCATACTATTTTTTTTTTCCCAATCATATTCTTTGTTATAGTTTTTTTCAATAAATTCATTTGGGTGAAATGGAGCATTCTGTTCAAAATTACTTAAATAGGAGACCTAAAAGAAAACTTTCAGATAGCATTAATCTCTTTCATATATTGCTGAGACTTCGAGGCAGATAGCTCATTTTCTTATATTGCTGAAACTGAAGCTGACACTGCAAGTATCAGCCAGTATGGTATTCTTCAGATTTGAATAGTTCATAGTTAGGTTCGATATAAGACAAATGGTTATATATTTAATCAGTTTTCAAAAAAGTTGTCCGACTTGTGCTTATTGGATTAATGTGATTGTCTGACTTGTTTGAGTTTTCTGTGAGCGCATGTCAGATGTTTTGCAGTTAATGCATGCCTAGTCTCTTATAGATTATCCTTTTTCAAGTGGTAGCTACCTTTTAAAATTTACTCCATGCCATGTTAGAGGTGTTTAATGTGGCTAATTTGAAGAAGTTCTGAAATTATGATATTTCCGCTTAAAATTTATATCTAAATTCTTCTCTTGAAAGCATACATGATTTTAGCTGCCTTGAATGAATAATTTTCTGCATTTTATTTGATGTGAAAATGTTCTGAAGGAACTAGACATTTGGTGCGCTTTCTGTCTCTTTCTAAGAACAATTTGTTTTTATTTTTTATTTTGCTATTGTCTTTGTGATGTACCATTGAGTCATACACTAATGTTCCACTTTTTGATGTCTCAAACTTCTCAAAACAATTAGTCAGTGGTCACTAATCCCCGTGTCTGGAAAACAAAAACAACATTTCTATTTTATTTTATTTTTTTAAATTTTTTTAAATTCTTGACCTTTTGGGGCTTTGCTAATTTATTATCAATAAATTTACATGATCTCATATCATGTTTTGTCTTTTTCTTATTAATGTTGGATAGTTTAAATGTAAATTGGAAAATTGGAACGAGTTCTGTGGAGTATTGCAGTAAGTTAATTAAGTAGTAATATCTAAGCCCTACTCTTTTGCTCTGATTAAAACTTAAAGTAGAAAATTTTCAATTGAATATTATGGACTGAAAATACTATATTTCAGTCTAATGTTTTTTTCAACATTCACTTGAGCAAGTAAAATATAAATGTATTTAATCATTTTAACCTTATGTTAGTTATAGATTCTGTAAGAATACTCATCTCCCTGATCTAAGGAAATAAAGGTAGTGAGGTGTGTGAAAAATGGGTATTTTTTTTTCTTTAACTTACCAACTAATAAATTAAACTTTTCAATTTGAATATAAATTCATAGAAGTTGAGCATCTAGTGGTTAATGCTTAAGTTTCTGATAGTAGAACTCTGTTTCTATTTCTAATTGTTATGATAAAAAACAATTTCACAGATACTTAGATATACTTGGACCCCAGTTGTTAATTTCATCTCTTAACCTGGTGCTTGGTTCTATTTTAGCTATTTGTTCTTCTCACCCTGTTTTAATTTTATCAAGTGTGTCTAAGTTTCTACCTCCCATCCTTCTGGTACAGTTGGCTGTGTGGTGGAGCCACGGGTCGAGAGCACACTTGGTCAAGTATTTCTGGTCATAGTTGTGGGCGTTACAAAGAAGAATCAGAACAGAAAGCTGAGCGAGCAAAACGGGATTTGTATCGGTATATGCACTATCATAATCGGTATAAAGCTCATACAGATTCTTTTAAGCTTGAAAGCAAATTGAAGGAATCTATACAAGAGAAAATCTCGATTTCGGAAGAGAGGGAATCCATGCTTAGAGATTTTAGTTGGGTAAACAATGGACTGTCTAGACTTTTCAGATCAAGGCGGGTTCTATCATACTCATACCCATTTGCATTCTATATGTTTGGGGATGAGCTATTCAAAGATGAGATGACTGAGGCGGAACGAGAGATAAAGCAGCATCTATTTGAGGACCAGCAACAGCAACTTGAGGCAAATGTTGAGAAGCTCTCCAAGTTTTTGGAGGAGCCGTTTGAGCAATATACTAAAGATAAGGTAATGGAGATACGGATGCAAGTTATCAATCTGTCAGTTATCACCGATACCCTCTGCAAGAAGATGTAAGTGCACTTGAACAATCTGTTCTTCTAAATGTGTGTAAAGCTCTTGGAACTCATGTCCTATTTTCATGCAGGTATGACTGTATTGAGAATGATCTTCTGGGTTCTCTTGAACTGGGTATCCACAATATTGCTCCTTACAAGTCGAAGGGCATCGAGAAGGCACTGGAGCTTTCTGCTTGTTGGAACAGCAAAGCAAATACTTCAGACAAATATCCACCATCAGACTGTGGTACAAGTGGTAAAGTGGAGAGTACTTTTCTACACTTTTCATATTCATCATTGTCAGTTCACAAATTTTGATTTTGATGTGTCAATCTCTTAACAATGCCCCTTATTGAATTGAAAATATGCATTTATTATCTACATCTCTTAATCAAGATTATTTCTCATTCATGGTCTTTCTTTGGGTTGTGCCTATTTGATGTGGAGTTTTTTGAATTCTTTTCAGACTATTTCTGAGACAGTATATTAGGGAAACTACCTGCAATACTGCTTTTAGATGAATACTGGGTATAAAAGTTTTACTGATGGTACAAATGCAGGAGGATTGTCGGAATGTGAACGACTGACTAGCTTGAATTCCGAGGAGAACGGATGCTCTTCTAGGAAACGTGCCAGAGCAGAAGTTGTTACTGGCAACTTTTTTGACCTTAACTTGCCTGCCGAGGTTGTTGATAGAAACTGATCTCTGGAAGTTACGTGGTTATAAAGTACAGTTGTACAGATCTGACATAAAACATTAGCTTCGTTAGCATGGGGACAGGCAATCGAGGATTAGTAATCCTAGAGGTGCTTTTTAACTCTTTTTTTACTCTTTGTTCATCATGTTTTGTCTCAGGTTGAATATATTGTCTTCGGCTGAGCTATATTTTGAAAGAGAATCTCTTTTTAAGTTCACGTTCTTTCTTTCCATTTTGCTTTTGAAAATCCGCAAATTGTACATAGCCGTGATATCGGGCTTTGATGGTATTTGGAATGTAAATGCTTTAATTCTCAGCCTGAGACAGACAAGAGAGTATATGCATTTTCAGATAGTCTAATATTTTCTTTGGCTGGAAGTAAAATTTCTATGCTGTCTTCATTTG

mRNA sequence

ACCAGATATTCCATTGCACCAAAACGGCGTCGTTTTATGGAAGGATTAATACGACGTCGTATAACCTTTGCTCTCTTCTCTTTTTATCTTCTCTCTCACAAATTTTGCTCTCTTTTTCTCTTTTTATTTATTTATATTTATTTTATATTTATATTTTACTTATTTCATCGGAAGAACTCCCACCATTTAGTCGTTGTCCTTTCACTCTCTTCGCCGGATTTTCCAACGATCTTCCCGGAGGGAAACGTTCTCCGGCGTTTCCCGATTTCTCCGGATCCGATTTATTCATCGAATCTCGTTATCGATTGTTTCGTTTTGCTTTTCTTTTTCCCCTTTTTTGTATTACCCACTCAGCTTTGCCTTTCTTCTTTCAATCTGACTTCAATTTCTTCTTCTCTTTCCCCAATTCCCTTTCCCTATTTCTCAATGGACGACTGTATCAGCAGCGATGAGGACTACTATGACTCCGATCGAGATTCTCTACATGGTCTTGAGAACGAGGACTCTGAAATTCAGTGGGTTCCTAAAGGTCCCGCGACTAAGGTTATTACGAAAGAGTCTCTCCTTGCGGCACAGAAGGAGGATATGCGTAGAGTAATGGACTTGCTATCGTTGAGGGAGTACCATGCTCGGACCTTACTCATTCATTACCGATGGGATGTAGAGAAATTGTTTGCAGTACTTGTGGAGAAAGGGAAAAGTCATTTGTTTGCTACAGCTGGTGTTACTATGATTGAGAATCGAAGTAATAGTTCCTCTGAGCCTTCTTCCATGGTGATGTGTGATATCTGCATGGAGGAAGTACATGACTACGATGCAACAAGAGTGGATTGTGGCCATTGCTTTTGTAATAACTGTTGGACTGAACATTTCATTGTGAAAATAAATGAGGGTCAAAGTCGGAGAATCAGGTGCATGGCCCACAAATGTAATGCGATTTGTGATGAAGCTGTCGTTAGGAATCTAGTTAGTAAAAGGCATCCAGATCTTGCTAATAAATTTGATCGTTTTCTTCTTGAATCATACATTGAGGATAACAAAAGAGTCAAGTGGTGTCCAAGTACTCCTCATTGTGGCAATGCTATACGCGTGGAGGATGATGAGTTTTGTGAAGTAGAATGTTCGTGTGGTATTCAGTTTTGTTTCAGTTGCTTATCAGAAGCTCACTCACCGTGTTCATGTTTGATGTGGGAACTTTGGATTAAGAAGTGCCGAGATGAATCTGAAACTGTTAACTGGATTACAGTACATACAAAGCCCTGCCCAAAGTGCCATAAACCTGTCGAGAAGAATGGAGGTTGTAACCTTGTCAGCTGCATCTGTGGTCAGGCATTTTGTTGGCTGTGTGGTGGAGCCACGGGTCGAGAGCACACTTGGTCAAGTATTTCTGGTCATAGTTGTGGGCGTTACAAAGAAGAATCAGAACAGAAAGCTGAGCGAGCAAAACGGGATTTGTATCGGTATATGCACTATCATAATCGGTATAAAGCTCATACAGATTCTTTTAAGCTTGAAAGCAAATTGAAGGAATCTATACAAGAGAAAATCTCGATTTCGGAAGAGAGGGAATCCATGCTTAGAGATTTTAGTTGGGTAAACAATGGACTGTCTAGACTTTTCAGATCAAGGCGGGTTCTATCATACTCATACCCATTTGCATTCTATATGTTTGGGGATGAGCTATTCAAAGATGAGATGACTGAGGCGGAACGAGAGATAAAGCAGCATCTATTTGAGGACCAGCAACAGCAACTTGAGGCAAATGTTGAGAAGCTCTCCAAGTTTTTGGAGGAGCCGTTTGAGCAATATACTAAAGATAAGGTAATGGAGATACGGATGCAAGTTATCAATCTGTCAGTTATCACCGATACCCTCTGCAAGAAGATGTATGACTGTATTGAGAATGATCTTCTGGGTTCTCTTGAACTGGGTATCCACAATATTGCTCCTTACAAGTCGAAGGGCATCGAGAAGGCACTGGAGCTTTCTGCTTGTTGGAACAGCAAAGCAAATACTTCAGACAAATATCCACCATCAGACTGTGGTACAAGTGGAGGATTGTCGGAATGTGAACGACTGACTAGCTTGAATTCCGAGGAGAACGGATGCTCTTCTAGGAAACGTGCCAGAGCAGAAGTTGTTACTGGCAACTTTTTTGACCTTAACTTGCCTGCCGAGGTTGTTGATAGAAACTGATCTCTGGAAGTTACGTGGTTATAAAGTACAGTTGTACAGATCTGACATAAAACATTAGCTTCGTTAGCATGGGGACAGGCAATCGAGGATTAGTAATCCTAGAGGTGCTTTTTAACTCTTTTTTTACTCTTTGTTCATCATGTTTTGTCTCAGGTTGAATATATTGTCTTCGGCTGAGCTATATTTTGAAAGAGAATCTCTTTTTAAGTTCACGTTCTTTCTTTCCATTTTGCTTTTGAAAATCCGCAAATTGTACATAGCCGTGATATCGGGCTTTGATGGTATTTGGAATGTAAATGCTTTAATTCTCAGCCTGAGACAGACAAGAGAGTATATGCATTTTCAGATAGTCTAATATTTTCTTTGGCTGGAAGTAAAATTTCTATGCTGTCTTCATTTG

Coding sequence (CDS)

ATGGAAGGATTAATACGACGTCGTATAACCTTTGCTCTCTTCTCTTTTTATCTTCTCTCTCACAAATTTTGCTCTCTTTTTCTCTTTTTATTTATTTATATTTATTTTATATTTATATTTTACTTATTTCATCGGAAGAACTCCCACCATTTAGTCGTTGTCCTTTCACTCTCTTCGCCGGATTTTCCAACGATCTTCCCGGAGGGAAACGTTCTCCGGCGTTTCCCGATTTCTCCGGATCCGATTTATTCATCGAATCTCGTTATCGATTGTTTCGTTTTGCTTTTCTTTTTCCCCTTTTTTGTATTACCCACTCAGCTTTGCCTTTCTTCTTTCAATCTGACTTCAATTTCTTCTTCTCTTTCCCCAATTCCCTTTCCCTATTTCTCAATGGACGACTGTATCAGCAGCGATGAGGACTACTATGACTCCGATCGAGATTCTCTACATGGTCTTGAGAACGAGGACTCTGAAATTCAGTGGGTTCCTAAAGGTCCCGCGACTAAGGTTATTACGAAAGAGTCTCTCCTTGCGGCACAGAAGGAGGATATGCGTAGAGTAATGGACTTGCTATCGTTGAGGGAGTACCATGCTCGGACCTTACTCATTCATTACCGATGGGATGTAGAGAAATTGTTTGCAGTACTTGTGGAGAAAGGGAAAAGTCATTTGTTTGCTACAGCTGGTGTTACTATGATTGAGAATCGAAGTAATAGTTCCTCTGAGCCTTCTTCCATGGTGATGTGTGATATCTGCATGGAGGAAGTACATGACTACGATGCAACAAGAGTGGATTGTGGCCATTGCTTTTGTAATAACTGTTGGACTGAACATTTCATTGTGAAAATAAATGAGGGTCAAAGTCGGAGAATCAGGTGCATGGCCCACAAATGTAATGCGATTTGTGATGAAGCTGTCGTTAGGAATCTAGTTAGTAAAAGGCATCCAGATCTTGCTAATAAATTTGATCGTTTTCTTCTTGAATCATACATTGAGGATAACAAAAGAGTCAAGTGGTGTCCAAGTACTCCTCATTGTGGCAATGCTATACGCGTGGAGGATGATGAGTTTTGTGAAGTAGAATGTTCGTGTGGTATTCAGTTTTGTTTCAGTTGCTTATCAGAAGCTCACTCACCGTGTTCATGTTTGATGTGGGAACTTTGGATTAAGAAGTGCCGAGATGAATCTGAAACTGTTAACTGGATTACAGTACATACAAAGCCCTGCCCAAAGTGCCATAAACCTGTCGAGAAGAATGGAGGTTGTAACCTTGTCAGCTGCATCTGTGGTCAGGCATTTTGTTGGCTGTGTGGTGGAGCCACGGGTCGAGAGCACACTTGGTCAAGTATTTCTGGTCATAGTTGTGGGCGTTACAAAGAAGAATCAGAACAGAAAGCTGAGCGAGCAAAACGGGATTTGTATCGGTATATGCACTATCATAATCGGTATAAAGCTCATACAGATTCTTTTAAGCTTGAAAGCAAATTGAAGGAATCTATACAAGAGAAAATCTCGATTTCGGAAGAGAGGGAATCCATGCTTAGAGATTTTAGTTGGGTAAACAATGGACTGTCTAGACTTTTCAGATCAAGGCGGGTTCTATCATACTCATACCCATTTGCATTCTATATGTTTGGGGATGAGCTATTCAAAGATGAGATGACTGAGGCGGAACGAGAGATAAAGCAGCATCTATTTGAGGACCAGCAACAGCAACTTGAGGCAAATGTTGAGAAGCTCTCCAAGTTTTTGGAGGAGCCGTTTGAGCAATATACTAAAGATAAGGTAATGGAGATACGGATGCAAGTTATCAATCTGTCAGTTATCACCGATACCCTCTGCAAGAAGATGTATGACTGTATTGAGAATGATCTTCTGGGTTCTCTTGAACTGGGTATCCACAATATTGCTCCTTACAAGTCGAAGGGCATCGAGAAGGCACTGGAGCTTTCTGCTTGTTGGAACAGCAAAGCAAATACTTCAGACAAATATCCACCATCAGACTGTGGTACAAGTGGAGGATTGTCGGAATGTGAACGACTGACTAGCTTGAATTCCGAGGAGAACGGATGCTCTTCTAGGAAACGTGCCAGAGCAGAAGTTGTTACTGGCAACTTTTTTGACCTTAACTTGCCTGCCGAGGTTGTTGATAGAAACTGA

Protein sequence

MEGLIRRRITFALFSFYLLSHKFCSLFLFLFIYIYFIFIFYLFHRKNSHHLVVVLSLSSPDFPTIFPEGNVLRRFPISPDPIYSSNLVIDCFVLLFFFPFFVLPTQLCLSSFNLTSISSSLSPIPFPYFSMDDCISSDEDYYDSDRDSLHGLENEDSEIQWVPKGPATKVITKESLLAAQKEDMRRVMDLLSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNSSSEPSSMVMCDICMEEVHDYDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGIQFCFSCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSFKLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELFKDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFEQYTKDKVMEIRMQVINLSVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCGTSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN
Homology
BLAST of Lsi10G003400 vs. ExPASy Swiss-Prot
Match: Q84RR2 (Probable E3 ubiquitin-protein ligase ARI2 OS=Arabidopsis thaliana OX=3702 GN=ARI2 PE=2 SV=1)

HSP 1 Score: 860.5 bits (2222), Expect = 1.3e-248
Identity = 426/597 (71.36%), Postives = 491/597 (82.24%), Query Frame = 0

Query: 131 MDDCISSDED--YYDSDRDSLHGLENEDS-EIQWVPKGPATKVITKESLLAAQKEDMRRV 190
           MDD +S +E+  YY SD++SL+G++N++S  I    +    KVITKESLLAAQ+ED+RRV
Sbjct: 1   MDDNLSGEEEDYYYSSDQESLNGIDNDESVSIPVSSRSNTVKVITKESLLAAQREDLRRV 60

Query: 191 MDLLSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRS--NSSSEPSS 250
           M+LLS++E+HARTLLIHYRWDVEKLFAVLVEKGK  LF+ AGVT++EN+S  +S S  SS
Sbjct: 61  MELLSVKEHHARTLLIHYRWDVEKLFAVLVEKGKDSLFSGAGVTLLENQSCDSSVSGSSS 120

Query: 251 MVMCDICMEEVHDYDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEA 310
           M+ CDIC+E+V  Y  TR+DCGH FCNNCWT HF VKINEGQS+RI CMAHKCNAICDE 
Sbjct: 121 MMSCDICVEDVPGYQLTRMDCGHSFCNNCWTGHFTVKINEGQSKRIICMAHKCNAICDED 180

Query: 311 VVRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCG 370
           VVR LVSK  PDLA KFDRFLLESYIEDNK VKWCPSTPHCGNAIRVEDDE CEVECSCG
Sbjct: 181 VVRALVSKSQPDLAEKFDRFLLESYIEDNKMVKWCPSTPHCGNAIRVEDDELCEVECSCG 240

Query: 371 IQFCFSCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLV 430
           +QFCFSC S+AHSPCSC+MWELW KKC DESETVNWITVHTKPCPKCHKPVEKNGGCNLV
Sbjct: 241 LQFCFSCSSQAHSPCSCVMWELWRKKCFDESETVNWITVHTKPCPKCHKPVEKNGGCNLV 300

Query: 431 SCICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKA 490
           +C+C Q+FCWLCG ATGR+HTW+ ISGHSCGR++E+ E++ ERAKRDL RYMHYHNRYKA
Sbjct: 301 TCLCRQSFCWLCGEATGRDHTWARISGHSCGRFQEDKEKQMERAKRDLKRYMHYHNRYKA 360

Query: 491 HTDSFKLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMF 550
           H DS KLE+KL  +I +K+SISE+RE  L+DFSW  NGL RLFRSRRVLSYSYPFAFYMF
Sbjct: 361 HIDSSKLEAKLSNNISKKVSISEKRELQLKDFSWATNGLHRLFRSRRVLSYSYPFAFYMF 420

Query: 551 GDELFKDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFEQYTKDKVMEIRMQVIN 610
           GDELFKDEM+  EREIKQ+LFEDQQQQLEANVEKLSKFLEEPF+Q+  DKVM+IR+QVIN
Sbjct: 421 GDELFKDEMSSEEREIKQNLFEDQQQQLEANVEKLSKFLEEPFDQFADDKVMQIRIQVIN 480

Query: 611 LSVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYP 670
           LSV  DTLC+ MY+CIENDLLGSL+LGIHNI PY+S GIE+A +  +  NSK        
Sbjct: 481 LSVAVDTLCENMYECIENDLLGSLQLGIHNITPYRSNGIERASDFYSSQNSKEAVGQS-- 540

Query: 671 PSDCGTSGGLSECERLTSLNSEENGCSSRKRARAEVVTGN----FFDLNLPAEVVDR 719
            SDCG +  L +   L S  SE+  CSS KRAR +    N      DLNLPAE ++R
Sbjct: 541 -SDCGWTSRLDQA--LESGKSEDTSCSSGKRARIDESYRNSQTTLLDLNLPAEAIER 592

BLAST of Lsi10G003400 vs. ExPASy Swiss-Prot
Match: Q949V6 (Probable E3 ubiquitin-protein ligase ARI1 OS=Arabidopsis thaliana OX=3702 GN=ARI1 PE=2 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 1.6e-246
Identity = 419/602 (69.60%), Postives = 505/602 (83.89%), Query Frame = 0

Query: 131 MDDCISSDED--YYDSDRDSLHGLENEDSEIQWV-PKGPATKVITKESLLAAQKEDMRRV 190
           MDD  S++E+  YY SD+DSL G++NE+SE+Q +  K   T+VIT+ESLLAAQ+ED+ RV
Sbjct: 1   MDDYFSAEEEACYYSSDQDSLDGIDNEESELQPLSSKRSNTQVITQESLLAAQREDLLRV 60

Query: 191 MDLLSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNSSSEP-SSM 250
           M+LLS++E+HARTLLIHY+WDVEKLFAV VEKGK  LF+ AGVT+ + +  +SS P SS 
Sbjct: 61  MELLSIKEHHARTLLIHYQWDVEKLFAVFVEKGKDSLFSGAGVTVFDYQYGNSSFPQSSQ 120

Query: 251 VMCDICMEEVHDYDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAV 310
           + CD+CME++     TR+DCGHCFCNNCWTEHF V+INEGQS+RIRCMAH+CNAICDE +
Sbjct: 121 MSCDVCMEDLPGDHMTRMDCGHCFCNNCWTEHFTVQINEGQSKRIRCMAHQCNAICDEDI 180

Query: 311 VRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGI 370
           VR+LVSK+ PDLA KFDR+LLESYIEDN+ VKWCPSTPHCGNAIR EDD+ CEVECSCG+
Sbjct: 181 VRSLVSKKRPDLAAKFDRYLLESYIEDNRMVKWCPSTPHCGNAIRAEDDKLCEVECSCGL 240

Query: 371 QFCFSCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVS 430
           QFCFSCL +AHSPCSCLMWELW KKCRDESET+NWITVHTK CPKC+KPVEKNGGCNLV 
Sbjct: 241 QFCFSCLCQAHSPCSCLMWELWRKKCRDESETINWITVHTKLCPKCYKPVEKNGGCNLVR 300

Query: 431 CICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAH 490
           CICGQ FCWLCGGATG +HT+ SI+GHSCGRY+++ E++ ERAKRDL RY HYH+RYKAH
Sbjct: 301 CICGQCFCWLCGGATGSDHTYRSIAGHSCGRYQDDKEKQMERAKRDLNRYTHYHHRYKAH 360

Query: 491 TDSFKLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFG 550
           TDS KLE KL+++I EK+S SE+RE  L+DFSWV NGL RLFRSRRVLSYSY FA+YMFG
Sbjct: 361 TDSSKLEDKLRDTIHEKVSKSEKRELKLKDFSWVTNGLDRLFRSRRVLSYSYAFAYYMFG 420

Query: 551 DELFKDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFEQYTKDKVMEIRMQVINL 610
           +E+FKDEMT  EREIK++LFEDQQQQLE+NVEKLS+FLEEPF++++ DKVM IR+Q+INL
Sbjct: 421 EEMFKDEMTPEEREIKKNLFEDQQQQLESNVEKLSQFLEEPFDEFSNDKVMAIRIQIINL 480

Query: 611 SVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPP 670
           SV  DTLCKKMY+CIENDLLGSL+LGIHNI+PY+SKGIE+A +  A WNSK   +DK+ P
Sbjct: 481 SVAVDTLCKKMYECIENDLLGSLQLGIHNISPYRSKGIEQAAQFYASWNSK--DADKFQP 540

Query: 671 SDCGTSGGLSECERLT-SLNSEENGC-SSRKRARAEVVTGNF-------FDLNLPAEVVD 720
            D GTSG  S  E+ + S +SE+  C SS+KR + E   G+F        DLNLPA+ VD
Sbjct: 541 LDSGTSGVTSRPEQASGSRSSEDTICSSSQKRPKKE---GSFLNNKVTLLDLNLPADFVD 597

BLAST of Lsi10G003400 vs. ExPASy Swiss-Prot
Match: Q9LVX0 (Probable E3 ubiquitin-protein ligase ARI3 OS=Arabidopsis thaliana OX=3702 GN=ARI3 PE=2 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 1.5e-183
Identity = 322/551 (58.44%), Postives = 419/551 (76.04%), Query Frame = 0

Query: 132 DDCISSDEDYYDSDRDSLHGLEN-EDSEIQWVP---KGPATKVITKESLLAAQKEDMRRV 191
           DD +  D+DY + + ++    +N  ++E+   P       ++VI KESL+AAQKE + RV
Sbjct: 3   DDYMMLDDDYGEEEDENYSEDDNYSEAEVDLQPVTSTKSTSQVIKKESLVAAQKEILVRV 62

Query: 192 MDLLSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNSSSEPSSMV 251
           M+LLS++E  ARTLLI+Y+W+VEKLF+V  ++GK  +F+ AG+T+      +S +    +
Sbjct: 63  MELLSVKENQARTLLIYYQWNVEKLFSVFADQGKDRMFSCAGLTVFVPSLVTSKK---TM 122

Query: 252 MCDICME-EVHDYDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAV 311
            CD+CME ++     TR++CGH FCN+CW  HF VKINEG+S+RI CMAH+C AICDE V
Sbjct: 123 KCDVCMEDDLPSNVMTRMECGHRFCNDCWIGHFTVKINEGESKRILCMAHECKAICDEDV 182

Query: 312 VRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDD--EFCEVECSC 371
           VR LVS   P+LA+++DRFL+ESY+EDN  VKWCPS PHCG+AIR  +D  +  EV CSC
Sbjct: 183 VRKLVS---PELADRYDRFLIESYVEDNNMVKWCPSKPHCGSAIRKIEDGHDVVEVGCSC 242

Query: 372 GIQFCFSCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNL 431
           G+QFCFSCLSE+HSPCSCLMW+LW KKC DESETVNWITV+TK CPKC KP++K  GCNL
Sbjct: 243 GLQFCFSCLSESHSPCSCLMWKLWKKKCEDESETVNWITVNTKLCPKCSKPIQKRDGCNL 302

Query: 432 VSCICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYK 491
           ++C CGQ FCWLCG ATGR+HT++SI+GHSCGRYK+E  ++ ERA+RDL RY HYH RYK
Sbjct: 303 MTCKCGQHFCWLCGQATGRDHTYTSIAGHSCGRYKDEKVRQLERAQRDLDRYTHYHYRYK 362

Query: 492 AHTDSFKLESKLKESIQEK-ISISEER-ESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAF 551
           AH DS KLE KL++SI EK +S SE + + + +++SWV + ++RLF SRR+LS SYPFAF
Sbjct: 363 AHIDSLKLEDKLRKSILEKAVSNSETKDQKVFKEYSWVTDAVNRLFISRRILSQSYPFAF 422

Query: 552 YMFGDELFKDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFEQYTKDKVMEIRMQ 611
           YMFG+ELFKDEM+E EREIK++LFEDQQQQLE NVEKLSK LEEPF++Y  +KV+E+   
Sbjct: 423 YMFGEELFKDEMSEKEREIKKNLFEDQQQQLEGNVEKLSKILEEPFDEYDHEKVVEMMRH 482

Query: 612 VINLSVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSD 671
           + NL+ + D LCK+MY+CIEN+LLG ++ G HNIAPY+SKGIE+A E  A          
Sbjct: 483 LTNLTAVVDNLCKEMYECIENELLGPIQFGNHNIAPYRSKGIEQATEFCA---------- 535

Query: 672 KYPPSDCGTSG 674
               SDCG+SG
Sbjct: 543 --ESSDCGSSG 535

BLAST of Lsi10G003400 vs. ExPASy Swiss-Prot
Match: Q9LVW9 (Putative E3 ubiquitin-protein ligase ARI4 OS=Arabidopsis thaliana OX=3702 GN=ARI4 PE=5 SV=2)

HSP 1 Score: 642.1 bits (1655), Expect = 7.4e-183
Identity = 317/526 (60.27%), Postives = 406/526 (77.19%), Query Frame = 0

Query: 132 DDCISSDEDYYDSDRDSLHGLENEDSEIQWVPKGPATKVITKESLLAAQKEDMRRVMDLL 191
           D+C  S+ D +D    +      E+S++Q   + P ++VITKE+L+AAQKE + +VM+ L
Sbjct: 13  DNCYPSEFDDHDQMCSNA-----EESDLQH-SREPTSQVITKEALVAAQKEVLVKVMEFL 72

Query: 192 SLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNSSSEPSSMVMCDI 251
           S+ E  ARTLLI Y+W+V+KLF+V  ++GK  LF+ AG+T+ +      S     + CDI
Sbjct: 73  SVTENQARTLLIQYQWNVDKLFSVYTDQGKDVLFSRAGLTVFD-----PSLTKKTMKCDI 132

Query: 252 CMEE-VHDYDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 311
           CMEE +  Y  TR++CGH FCN+CW EHF V+INEG+ +RIRCMA+KCN ICDEA  R L
Sbjct: 133 CMEEDLSKYAMTRMECGHRFCNDCWKEHFTVRINEGEGKRIRCMAYKCNTICDEA--RQL 192

Query: 312 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIR--VEDDEFCEVECSCGIQF 371
           VS    +LA KFDRFL+ESY+EDN  VKWCPSTPHCGNAIR   +D +  EVECSCG+QF
Sbjct: 193 VS---TELAEKFDRFLIESYVEDNNMVKWCPSTPHCGNAIRNIKDDGDVDEVECSCGLQF 252

Query: 372 CFSCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCI 431
           CFSCLSE+HSPCSCLMW+LW KKC DESETVNW+TV+TK CPKC KP++K  GCN ++C 
Sbjct: 253 CFSCLSESHSPCSCLMWKLWKKKCEDESETVNWMTVNTKLCPKCSKPIQKRDGCNHMTCK 312

Query: 432 CGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTD 491
           CGQ FCWLCG ATGR+H++SSI+GHSCGRYKEE  ++ ERA+RDL RY HYH RYKAH D
Sbjct: 313 CGQHFCWLCGQATGRDHSYSSIAGHSCGRYKEEKVRQLERAQRDLDRYTHYHYRYKAHID 372

Query: 492 SFKLESKLKESIQEKISISEER--ESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFG 551
           S KLE KLK+SI +K  ++ E   + + +++SW+ + ++RLFRSRR+LSYSYPF FYMFG
Sbjct: 373 SLKLEDKLKKSILKKAVLNSETKDQKVFKEYSWIIDAVNRLFRSRRILSYSYPFVFYMFG 432

Query: 552 DELFKDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFEQYTKDKVMEIRMQVINL 611
            ELFKD+M++ ER IK++LFEDQQQQLE NVE+LSK LEEPF++Y  +KV+E+   + NL
Sbjct: 433 KELFKDDMSDEERNIKKNLFEDQQQQLEGNVERLSKILEEPFDEYDHEKVVEMMRHLTNL 492

Query: 612 SVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSA 653
           + + D LCK+MY+CIEN+LLG L  GIHNIAPY+SKGIE+A E SA
Sbjct: 493 TAVVDNLCKEMYECIENELLGPLISGIHNIAPYRSKGIEQAAEFSA 522

BLAST of Lsi10G003400 vs. ExPASy Swiss-Prot
Match: Q94981 (E3 ubiquitin-protein ligase ariadne-1 OS=Drosophila melanogaster OX=7227 GN=ari-1 PE=1 SV=2)

HSP 1 Score: 243.0 bits (619), Expect = 1.0e-62
Identity = 146/448 (32.59%), Postives = 228/448 (50.89%), Query Frame = 0

Query: 169 KVITKESLLAAQKEDMRRVMDLLSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATA 228
           KV+T + ++  Q+E +     LL L     R LL H++WD EKL     +      F  A
Sbjct: 50  KVLTTDEIVQHQREIIDEANLLLKLPTPTTRILLNHFKWDKEKLLEKYFDDNTDEFFKCA 109

Query: 229 GVTMIENRSNSSSEPSSMVM---CDICMEEVHDYDATRVDCGHCFCNNCWTEHFIVKI-N 288
            V    N + +  + +S      C+IC  ++       ++CGH FC  CW E+   KI  
Sbjct: 110 HVINPFNATEAIKQKTSRSQCEECEICFSQLPPDSMAGLECGHRFCMPCWHEYLSTKIVA 169

Query: 289 EGQSRRIRCMAHKCNAICDEAVVRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTP 348
           EG  + I C AH C+ + D+  V NLV+     +  K+ + +  S++E N+ ++WCPS  
Sbjct: 170 EGLGQTISCAAHGCDILVDDVTVANLVTDAR--VRVKYQQLITNSFVECNQLLRWCPSV- 229

Query: 349 HCGNAIRVEDDEFCEVECSCGIQFCFSCLSEAHSPCSCLMWELWIKKCRDESETVNWITV 408
            C  A++V   E   V C CG  FCF+C    H P  C   + WIKKC D+SET NWI  
Sbjct: 230 DCTYAVKVPYAEPRRVHCKCGHVFCFACGENWHDPVKCRWLKKWIKKCDDDSETSNWIAA 289

Query: 409 HTKPCPKCHKPVEKNGGCNLVSC---ICGQAFCWLCGGATGREHTWS--SISGHSCGRYK 468
           +TK CP+C   +EK+GGCN + C    C   FCW+C G      +W     S ++C RY 
Sbjct: 290 NTKECPRCSVTIEKDGGCNHMVCKNQNCKNEFCWVCLG------SWEPHGSSWYNCNRYD 349

Query: 469 EESEQKA----ERAKRDLYRYMHYHNRYKAHTDSFKLESKLKESIQEKISISEERESMLR 528
           E+  + A    E+ +  L RY+HY+NRY  H  S K E+KL  S+++K+   ++      
Sbjct: 350 EDEAKTARDAQEKLRSSLARYLHYYNRYMNHMQSMKFENKLYASVKQKMEEMQQHNMSWI 409

Query: 529 DFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELFKDEMTEAEREIKQHLFEDQQQQLEA 588
           +  ++   +  L + R+ L Y+Y FA+Y+             ++  +  +FED Q+ LE+
Sbjct: 410 EVQFLKKAVDILCQCRQTLMYTYVFAYYL-------------KKNNQSMIFEDNQKDLES 469

Query: 589 NVEKLSKFLEEPFEQYTKDKVMEIRMQV 604
             E LS++LE      T + + +I+ +V
Sbjct: 470 ATEMLSEYLE---RDITSENLADIKQKV 472

BLAST of Lsi10G003400 vs. ExPASy TrEMBL
Match: A0A5D3CP89 (Putative E3 ubiquitin-protein ligase ARI2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G001360 PE=3 SV=1)

HSP 1 Score: 1192.2 bits (3083), Expect = 0.0e+00
Identity = 579/589 (98.30%), Postives = 583/589 (98.98%), Query Frame = 0

Query: 131 MDDCISSDEDYYDSDRDSLHGLENEDSEIQWVPKGPATKVITKESLLAAQKEDMRRVMDL 190
           MDDCISSDEDYYDSDRDSLHGLENEDSEIQ VPK P+TKVITKESLLAAQKEDMRRVMDL
Sbjct: 1   MDDCISSDEDYYDSDRDSLHGLENEDSEIQRVPKTPSTKVITKESLLAAQKEDMRRVMDL 60

Query: 191 LSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNSSSEPSSMVMCD 250
           LSLREYHARTLLIHYRWDVEKLFAVLVEKGK+HLFATAGVTMIENRSNSSSE SSMVMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLFAVLVEKGKNHLFATAGVTMIENRSNSSSEASSMVMCD 120

Query: 251 ICMEEVHDYDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 310
           ICMEEVH  DATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVR L
Sbjct: 121 ICMEEVHGNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRTL 180

Query: 311 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGIQFCF 370
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCG+QFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 371 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 430
           SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 431 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 490
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 491 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 550
           KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 551 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFEQYTKDKVMEIRMQVINLSVIT 610
           KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPF+QYTKDKVMEIRMQVINLSVIT
Sbjct: 421 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480

Query: 611 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 670
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 540

Query: 671 TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 720
           TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN
Sbjct: 541 TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 589

BLAST of Lsi10G003400 vs. ExPASy TrEMBL
Match: A0A1S3C9A1 (probable E3 ubiquitin-protein ligase ARI2 OS=Cucumis melo OX=3656 GN=LOC103498436 PE=3 SV=1)

HSP 1 Score: 1192.2 bits (3083), Expect = 0.0e+00
Identity = 579/589 (98.30%), Postives = 583/589 (98.98%), Query Frame = 0

Query: 131 MDDCISSDEDYYDSDRDSLHGLENEDSEIQWVPKGPATKVITKESLLAAQKEDMRRVMDL 190
           MDDCISSDEDYYDSDRDSLHGLENEDSEIQ VPK P+TKVITKESLLAAQKEDMRRVMDL
Sbjct: 1   MDDCISSDEDYYDSDRDSLHGLENEDSEIQRVPKTPSTKVITKESLLAAQKEDMRRVMDL 60

Query: 191 LSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNSSSEPSSMVMCD 250
           LSLREYHARTLLIHYRWDVEKLFAVLVEKGK+HLFATAGVTMIENRSNSSSE SSMVMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLFAVLVEKGKNHLFATAGVTMIENRSNSSSEASSMVMCD 120

Query: 251 ICMEEVHDYDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 310
           ICMEEVH  DATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVR L
Sbjct: 121 ICMEEVHGNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRTL 180

Query: 311 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGIQFCF 370
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCG+QFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 371 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 430
           SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 431 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 490
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 491 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 550
           KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 551 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFEQYTKDKVMEIRMQVINLSVIT 610
           KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPF+QYTKDKVMEIRMQVINLSVIT
Sbjct: 421 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480

Query: 611 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 670
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 540

Query: 671 TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 720
           TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN
Sbjct: 541 TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 589

BLAST of Lsi10G003400 vs. ExPASy TrEMBL
Match: A0A0A0LL32 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G238850 PE=3 SV=1)

HSP 1 Score: 1189.1 bits (3075), Expect = 0.0e+00
Identity = 577/589 (97.96%), Postives = 582/589 (98.81%), Query Frame = 0

Query: 131 MDDCISSDEDYYDSDRDSLHGLENEDSEIQWVPKGPATKVITKESLLAAQKEDMRRVMDL 190
           MDDCISSDEDYYDSDRDSLHGLENEDSEIQ VPK P+TKVITKESLLAAQKEDMRRVMDL
Sbjct: 1   MDDCISSDEDYYDSDRDSLHGLENEDSEIQRVPKTPSTKVITKESLLAAQKEDMRRVMDL 60

Query: 191 LSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNSSSEPSSMVMCD 250
           LSLREYHARTLLIHYRWDVEKLFAVLVEKGK+HLFATAGVTMIENRSN+SSE SSMVMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLFAVLVEKGKNHLFATAGVTMIENRSNTSSEASSMVMCD 120

Query: 251 ICMEEVHDYDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 310
           ICMEEVH  DATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVR L
Sbjct: 121 ICMEEVHGNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRTL 180

Query: 311 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGIQFCF 370
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCG+QFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 371 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 430
           SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 431 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 490
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 491 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 550
           KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 551 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFEQYTKDKVMEIRMQVINLSVIT 610
           KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPF+QYTKDKVMEIRMQVINLSVIT
Sbjct: 421 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480

Query: 611 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 670
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 540

Query: 671 TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 720
           TSGGLSECERLTSLNSEENGCSSRKRARAEVV GNFFDLNLPAEVVDRN
Sbjct: 541 TSGGLSECERLTSLNSEENGCSSRKRARAEVVAGNFFDLNLPAEVVDRN 589

BLAST of Lsi10G003400 vs. ExPASy TrEMBL
Match: A0A6J1J7N9 (probable E3 ubiquitin-protein ligase ARI2 OS=Cucurbita maxima OX=3661 GN=LOC111484213 PE=3 SV=1)

HSP 1 Score: 1156.0 bits (2989), Expect = 0.0e+00
Identity = 558/589 (94.74%), Postives = 574/589 (97.45%), Query Frame = 0

Query: 131 MDDCISSDEDYYDSDRDSLHGLENEDSEIQWVPKGPATKVITKESLLAAQKEDMRRVMDL 190
           M+DCISSDEDYYDSDR+SL GLENEDSEIQWV KGPATKVITKESLLAAQKEDMRRVM+L
Sbjct: 1   MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGPATKVITKESLLAAQKEDMRRVMEL 60

Query: 191 LSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNSSSEPSSMVMCD 250
           LSLRE+HARTLLIHYRWDVEKLFAVLVEKGK HLFATAGVTMIENRSN SSE SS+VMCD
Sbjct: 61  LSLREHHARTLLIHYRWDVEKLFAVLVEKGKGHLFATAGVTMIENRSNCSSETSSVVMCD 120

Query: 251 ICMEEVHDYDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 310
           ICMEEVH  DATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL
Sbjct: 121 ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180

Query: 311 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGIQFCF 370
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPS PHCGNAIRVEDDEFCEVECSCG+QFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 371 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 430
           SCLS+AHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 431 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 490
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 491 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 550
           KLESKLKESIQEKISISEE+ESMLRDFSWVNNGL RLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 551 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFEQYTKDKVMEIRMQVINLSVIT 610
           KDEMT+ EREI+QHLFEDQQQQLEANVEKLSKFLEEPF+QYTKD+VM+IRMQVINLSVIT
Sbjct: 421 KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT 480

Query: 611 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 670
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKAN +DKY PSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANNTDKYLPSDCG 540

Query: 671 TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 720
           TSGGLSE  RL+SLNS+ENGCSSRKRARAEVVTGN FDLNLPAEVVDRN
Sbjct: 541 TSGGLSERNRLSSLNSDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN 589

BLAST of Lsi10G003400 vs. ExPASy TrEMBL
Match: A0A6J1FVJ5 (probable E3 ubiquitin-protein ligase ARI2 OS=Cucurbita moschata OX=3662 GN=LOC111448849 PE=3 SV=1)

HSP 1 Score: 1155.2 bits (2987), Expect = 0.0e+00
Identity = 558/589 (94.74%), Postives = 575/589 (97.62%), Query Frame = 0

Query: 131 MDDCISSDEDYYDSDRDSLHGLENEDSEIQWVPKGPATKVITKESLLAAQKEDMRRVMDL 190
           M+DCISSDEDYYDSDR+SL GLENEDSEIQWV KG ATKVITKESLLAAQKEDMRRVM+L
Sbjct: 1   MEDCISSDEDYYDSDRESLQGLENEDSEIQWVSKGSATKVITKESLLAAQKEDMRRVMEL 60

Query: 191 LSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNSSSEPSSMVMCD 250
           LSLRE+HARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSN SSE SS+VMCD
Sbjct: 61  LSLREHHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNCSSETSSVVMCD 120

Query: 251 ICMEEVHDYDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 310
           ICMEEVH  DATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL
Sbjct: 121 ICMEEVHSNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180

Query: 311 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGIQFCF 370
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPS PHCGNAIRVEDDEFCEVECSCG+QFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSAPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 371 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 430
           SCLS+AHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSDAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 431 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 490
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 491 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 550
           KLESKLKESIQEKISISEE+ESMLRDFSWVNNGL RLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEEKESMLRDFSWVNNGLCRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 551 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFEQYTKDKVMEIRMQVINLSVIT 610
           KDEMT+ EREI+QHLFEDQQQQLEANVEKLSKFLEEPF+QYTKD+VM+IRMQVINLSVIT
Sbjct: 421 KDEMTQEEREIRQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMDIRMQVINLSVIT 480

Query: 611 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 670
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANT+DKY PSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTTDKYLPSDCG 540

Query: 671 TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 720
           TSGGLSE  RL+SLN++ENGCSSRKRARAEVVTGN FDLNLPAEVVDRN
Sbjct: 541 TSGGLSERNRLSSLNTDENGCSSRKRARAEVVTGNCFDLNLPAEVVDRN 589

BLAST of Lsi10G003400 vs. NCBI nr
Match: XP_038901258.1 (probable E3 ubiquitin-protein ligase ARI2 isoform X2 [Benincasa hispida])

HSP 1 Score: 1199.1 bits (3101), Expect = 0.0e+00
Identity = 579/589 (98.30%), Postives = 585/589 (99.32%), Query Frame = 0

Query: 131 MDDCISSDEDYYDSDRDSLHGLENEDSEIQWVPKGPATKVITKESLLAAQKEDMRRVMDL 190
           MDDC+SSDEDYYDSDRDSLHG+ENEDSEIQWVPK PATKVITKESLLAAQKEDMRRVMDL
Sbjct: 1   MDDCLSSDEDYYDSDRDSLHGIENEDSEIQWVPKAPATKVITKESLLAAQKEDMRRVMDL 60

Query: 191 LSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNSSSEPSSMVMCD 250
           LSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGV+MIENRSNSSSEPSSMVMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVSMIENRSNSSSEPSSMVMCD 120

Query: 251 ICMEEVHDYDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 310
           ICMEEVH  DATRVDC HCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL
Sbjct: 121 ICMEEVHGNDATRVDCDHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180

Query: 311 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGIQFCF 370
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCG+QFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 371 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 430
           SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 431 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 490
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 491 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 550
           KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 551 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFEQYTKDKVMEIRMQVINLSVIT 610
           KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPF+QYTKD+VMEIRMQVINLSVIT
Sbjct: 421 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMEIRMQVINLSVIT 480

Query: 611 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 670
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 540

Query: 671 TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 720
           TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN
Sbjct: 541 TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 589

BLAST of Lsi10G003400 vs. NCBI nr
Match: XP_038901257.1 (probable E3 ubiquitin-protein ligase ARI2 isoform X1 [Benincasa hispida])

HSP 1 Score: 1193.3 bits (3086), Expect = 0.0e+00
Identity = 579/593 (97.64%), Postives = 585/593 (98.65%), Query Frame = 0

Query: 131 MDDCISSDEDYYDSDRDSLHGLENEDSEIQWVPKGPATKVITKESLLAAQKEDMRRVMDL 190
           MDDC+SSDEDYYDSDRDSLHG+ENEDSEIQWVPK PATKVITKESLLAAQKEDMRRVMDL
Sbjct: 1   MDDCLSSDEDYYDSDRDSLHGIENEDSEIQWVPKAPATKVITKESLLAAQKEDMRRVMDL 60

Query: 191 LSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNSSSEPSSMVMCD 250
           LSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGV+MIENRSNSSSEPSSMVMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVSMIENRSNSSSEPSSMVMCD 120

Query: 251 ICMEEVHDYDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 310
           ICMEEVH  DATRVDC HCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL
Sbjct: 121 ICMEEVHGNDATRVDCDHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 180

Query: 311 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGIQFCF 370
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCG+QFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 371 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 430
           SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 431 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 490
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 491 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 550
           KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 551 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFEQYTKDKVMEIRMQVINLSVIT 610
           KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPF+QYTKD+VMEIRMQVINLSVIT
Sbjct: 421 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDRVMEIRMQVINLSVIT 480

Query: 611 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 670
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 540

Query: 671 TSG----GLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 720
           TSG    GLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN
Sbjct: 541 TSGKVERGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 593

BLAST of Lsi10G003400 vs. NCBI nr
Match: XP_008459262.1 (PREDICTED: probable E3 ubiquitin-protein ligase ARI2 [Cucumis melo] >KAA0045954.1 putative E3 ubiquitin-protein ligase ARI2 [Cucumis melo var. makuwa] >TYK13631.1 putative E3 ubiquitin-protein ligase ARI2 [Cucumis melo var. makuwa])

HSP 1 Score: 1192.2 bits (3083), Expect = 0.0e+00
Identity = 579/589 (98.30%), Postives = 583/589 (98.98%), Query Frame = 0

Query: 131 MDDCISSDEDYYDSDRDSLHGLENEDSEIQWVPKGPATKVITKESLLAAQKEDMRRVMDL 190
           MDDCISSDEDYYDSDRDSLHGLENEDSEIQ VPK P+TKVITKESLLAAQKEDMRRVMDL
Sbjct: 1   MDDCISSDEDYYDSDRDSLHGLENEDSEIQRVPKTPSTKVITKESLLAAQKEDMRRVMDL 60

Query: 191 LSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNSSSEPSSMVMCD 250
           LSLREYHARTLLIHYRWDVEKLFAVLVEKGK+HLFATAGVTMIENRSNSSSE SSMVMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLFAVLVEKGKNHLFATAGVTMIENRSNSSSEASSMVMCD 120

Query: 251 ICMEEVHDYDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 310
           ICMEEVH  DATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVR L
Sbjct: 121 ICMEEVHGNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRTL 180

Query: 311 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGIQFCF 370
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCG+QFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 371 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 430
           SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 431 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 490
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 491 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 550
           KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 551 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFEQYTKDKVMEIRMQVINLSVIT 610
           KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPF+QYTKDKVMEIRMQVINLSVIT
Sbjct: 421 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480

Query: 611 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 670
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 540

Query: 671 TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 720
           TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN
Sbjct: 541 TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 589

BLAST of Lsi10G003400 vs. NCBI nr
Match: XP_004153333.1 (probable E3 ubiquitin-protein ligase ARI2 isoform X1 [Cucumis sativus] >KGN61769.1 hypothetical protein Csa_005965 [Cucumis sativus])

HSP 1 Score: 1189.1 bits (3075), Expect = 0.0e+00
Identity = 577/589 (97.96%), Postives = 582/589 (98.81%), Query Frame = 0

Query: 131 MDDCISSDEDYYDSDRDSLHGLENEDSEIQWVPKGPATKVITKESLLAAQKEDMRRVMDL 190
           MDDCISSDEDYYDSDRDSLHGLENEDSEIQ VPK P+TKVITKESLLAAQKEDMRRVMDL
Sbjct: 1   MDDCISSDEDYYDSDRDSLHGLENEDSEIQRVPKTPSTKVITKESLLAAQKEDMRRVMDL 60

Query: 191 LSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNSSSEPSSMVMCD 250
           LSLREYHARTLLIHYRWDVEKLFAVLVEKGK+HLFATAGVTMIENRSN+SSE SSMVMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLFAVLVEKGKNHLFATAGVTMIENRSNTSSEASSMVMCD 120

Query: 251 ICMEEVHDYDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 310
           ICMEEVH  DATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVR L
Sbjct: 121 ICMEEVHGNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRTL 180

Query: 311 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGIQFCF 370
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCG+QFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 371 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 430
           SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 431 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 490
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 491 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 550
           KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 551 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFEQYTKDKVMEIRMQVINLSVIT 610
           KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPF+QYTKDKVMEIRMQVINLSVIT
Sbjct: 421 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480

Query: 611 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 670
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 540

Query: 671 TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 720
           TSGGLSECERLTSLNSEENGCSSRKRARAEVV GNFFDLNLPAEVVDRN
Sbjct: 541 TSGGLSECERLTSLNSEENGCSSRKRARAEVVAGNFFDLNLPAEVVDRN 589

BLAST of Lsi10G003400 vs. NCBI nr
Match: XP_031736976.1 (probable E3 ubiquitin-protein ligase ARI2 isoform X2 [Cucumis sativus])

HSP 1 Score: 1178.7 bits (3048), Expect = 0.0e+00
Identity = 574/589 (97.45%), Postives = 579/589 (98.30%), Query Frame = 0

Query: 131 MDDCISSDEDYYDSDRDSLHGLENEDSEIQWVPKGPATKVITKESLLAAQKEDMRRVMDL 190
           MDDCISSDEDYYDSDRDSLHGLENEDSEIQ VPK P+TKVITKESLLAAQKEDMRRVMDL
Sbjct: 1   MDDCISSDEDYYDSDRDSLHGLENEDSEIQRVPKTPSTKVITKESLLAAQKEDMRRVMDL 60

Query: 191 LSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNSSSEPSSMVMCD 250
           LSLREYHARTLLIHYRWDVEKLFAVLVEKGK+HLFATAGVTMIENRSN+SSE SSMVMCD
Sbjct: 61  LSLREYHARTLLIHYRWDVEKLFAVLVEKGKNHLFATAGVTMIENRSNTSSEASSMVMCD 120

Query: 251 ICMEEVHDYDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRNL 310
           ICMEEVH  DATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVR L
Sbjct: 121 ICMEEVHGNDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAVVRTL 180

Query: 311 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGIQFCF 370
           VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCG+QFCF
Sbjct: 181 VSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGLQFCF 240

Query: 371 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 430
           SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG
Sbjct: 241 SCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVSCICG 300

Query: 431 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 490
           QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF
Sbjct: 301 QAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAHTDSF 360

Query: 491 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 550
           KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF
Sbjct: 361 KLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFGDELF 420

Query: 551 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFEQYTKDKVMEIRMQVINLSVIT 610
           KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPF+QYTKDKVMEIRMQVINLSVIT
Sbjct: 421 KDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFDQYTKDKVMEIRMQVINLSVIT 480

Query: 611 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDCG 670
           DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDC 
Sbjct: 481 DTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPPSDC- 540

Query: 671 TSGGLSECERLTSLNSEENGCSSRKRARAEVVTGNFFDLNLPAEVVDRN 720
             GGLSECERLTSLNSEENGCSSRKRARAEVV GNFFDLNLPAEVVDRN
Sbjct: 541 --GGLSECERLTSLNSEENGCSSRKRARAEVVAGNFFDLNLPAEVVDRN 586

BLAST of Lsi10G003400 vs. TAIR 10
Match: AT2G16090.1 (RING/U-box superfamily protein )

HSP 1 Score: 860.5 bits (2222), Expect = 9.4e-250
Identity = 426/597 (71.36%), Postives = 491/597 (82.24%), Query Frame = 0

Query: 131 MDDCISSDED--YYDSDRDSLHGLENEDS-EIQWVPKGPATKVITKESLLAAQKEDMRRV 190
           MDD +S +E+  YY SD++SL+G++N++S  I    +    KVITKESLLAAQ+ED+RRV
Sbjct: 1   MDDNLSGEEEDYYYSSDQESLNGIDNDESVSIPVSSRSNTVKVITKESLLAAQREDLRRV 60

Query: 191 MDLLSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRS--NSSSEPSS 250
           M+LLS++E+HARTLLIHYRWDVEKLFAVLVEKGK  LF+ AGVT++EN+S  +S S  SS
Sbjct: 61  MELLSVKEHHARTLLIHYRWDVEKLFAVLVEKGKDSLFSGAGVTLLENQSCDSSVSGSSS 120

Query: 251 MVMCDICMEEVHDYDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEA 310
           M+ CDIC+E+V  Y  TR+DCGH FCNNCWT HF VKINEGQS+RI CMAHKCNAICDE 
Sbjct: 121 MMSCDICVEDVPGYQLTRMDCGHSFCNNCWTGHFTVKINEGQSKRIICMAHKCNAICDED 180

Query: 311 VVRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCG 370
           VVR LVSK  PDLA KFDRFLLESYIEDNK VKWCPSTPHCGNAIRVEDDE CEVECSCG
Sbjct: 181 VVRALVSKSQPDLAEKFDRFLLESYIEDNKMVKWCPSTPHCGNAIRVEDDELCEVECSCG 240

Query: 371 IQFCFSCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLV 430
           +QFCFSC S+AHSPCSC+MWELW KKC DESETVNWITVHTKPCPKCHKPVEKNGGCNLV
Sbjct: 241 LQFCFSCSSQAHSPCSCVMWELWRKKCFDESETVNWITVHTKPCPKCHKPVEKNGGCNLV 300

Query: 431 SCICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKA 490
           +C+C Q+FCWLCG ATGR+HTW+ ISGHSCGR++E+ E++ ERAKRDL RYMHYHNRYKA
Sbjct: 301 TCLCRQSFCWLCGEATGRDHTWARISGHSCGRFQEDKEKQMERAKRDLKRYMHYHNRYKA 360

Query: 491 HTDSFKLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMF 550
           H DS KLE+KL  +I +K+SISE+RE  L+DFSW  NGL RLFRSRRVLSYSYPFAFYMF
Sbjct: 361 HIDSSKLEAKLSNNISKKVSISEKRELQLKDFSWATNGLHRLFRSRRVLSYSYPFAFYMF 420

Query: 551 GDELFKDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFEQYTKDKVMEIRMQVIN 610
           GDELFKDEM+  EREIKQ+LFEDQQQQLEANVEKLSKFLEEPF+Q+  DKVM+IR+QVIN
Sbjct: 421 GDELFKDEMSSEEREIKQNLFEDQQQQLEANVEKLSKFLEEPFDQFADDKVMQIRIQVIN 480

Query: 611 LSVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYP 670
           LSV  DTLC+ MY+CIENDLLGSL+LGIHNI PY+S GIE+A +  +  NSK        
Sbjct: 481 LSVAVDTLCENMYECIENDLLGSLQLGIHNITPYRSNGIERASDFYSSQNSKEAVGQS-- 540

Query: 671 PSDCGTSGGLSECERLTSLNSEENGCSSRKRARAEVVTGN----FFDLNLPAEVVDR 719
            SDCG +  L +   L S  SE+  CSS KRAR +    N      DLNLPAE ++R
Sbjct: 541 -SDCGWTSRLDQA--LESGKSEDTSCSSGKRARIDESYRNSQTTLLDLNLPAEAIER 592

BLAST of Lsi10G003400 vs. TAIR 10
Match: AT4G34370.1 (RING/U-box superfamily protein )

HSP 1 Score: 853.6 bits (2204), Expect = 1.2e-247
Identity = 419/602 (69.60%), Postives = 505/602 (83.89%), Query Frame = 0

Query: 131 MDDCISSDED--YYDSDRDSLHGLENEDSEIQWV-PKGPATKVITKESLLAAQKEDMRRV 190
           MDD  S++E+  YY SD+DSL G++NE+SE+Q +  K   T+VIT+ESLLAAQ+ED+ RV
Sbjct: 1   MDDYFSAEEEACYYSSDQDSLDGIDNEESELQPLSSKRSNTQVITQESLLAAQREDLLRV 60

Query: 191 MDLLSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNSSSEP-SSM 250
           M+LLS++E+HARTLLIHY+WDVEKLFAV VEKGK  LF+ AGVT+ + +  +SS P SS 
Sbjct: 61  MELLSIKEHHARTLLIHYQWDVEKLFAVFVEKGKDSLFSGAGVTVFDYQYGNSSFPQSSQ 120

Query: 251 VMCDICMEEVHDYDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAV 310
           + CD+CME++     TR+DCGHCFCNNCWTEHF V+INEGQS+RIRCMAH+CNAICDE +
Sbjct: 121 MSCDVCMEDLPGDHMTRMDCGHCFCNNCWTEHFTVQINEGQSKRIRCMAHQCNAICDEDI 180

Query: 311 VRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGI 370
           VR+LVSK+ PDLA KFDR+LLESYIEDN+ VKWCPSTPHCGNAIR EDD+ CEVECSCG+
Sbjct: 181 VRSLVSKKRPDLAAKFDRYLLESYIEDNRMVKWCPSTPHCGNAIRAEDDKLCEVECSCGL 240

Query: 371 QFCFSCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVS 430
           QFCFSCL +AHSPCSCLMWELW KKCRDESET+NWITVHTK CPKC+KPVEKNGGCNLV 
Sbjct: 241 QFCFSCLCQAHSPCSCLMWELWRKKCRDESETINWITVHTKLCPKCYKPVEKNGGCNLVR 300

Query: 431 CICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAH 490
           CICGQ FCWLCGGATG +HT+ SI+GHSCGRY+++ E++ ERAKRDL RY HYH+RYKAH
Sbjct: 301 CICGQCFCWLCGGATGSDHTYRSIAGHSCGRYQDDKEKQMERAKRDLNRYTHYHHRYKAH 360

Query: 491 TDSFKLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFG 550
           TDS KLE KL+++I EK+S SE+RE  L+DFSWV NGL RLFRSRRVLSYSY FA+YMFG
Sbjct: 361 TDSSKLEDKLRDTIHEKVSKSEKRELKLKDFSWVTNGLDRLFRSRRVLSYSYAFAYYMFG 420

Query: 551 DELFKDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFEQYTKDKVMEIRMQVINL 610
           +E+FKDEMT  EREIK++LFEDQQQQLE+NVEKLS+FLEEPF++++ DKVM IR+Q+INL
Sbjct: 421 EEMFKDEMTPEEREIKKNLFEDQQQQLESNVEKLSQFLEEPFDEFSNDKVMAIRIQIINL 480

Query: 611 SVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPP 670
           SV  DTLCKKMY+CIENDLLGSL+LGIHNI+PY+SKGIE+A +  A WNSK   +DK+ P
Sbjct: 481 SVAVDTLCKKMYECIENDLLGSLQLGIHNISPYRSKGIEQAAQFYASWNSK--DADKFQP 540

Query: 671 SDCGTSGGLSECERLT-SLNSEENGC-SSRKRARAEVVTGNF-------FDLNLPAEVVD 720
            D GTSG  S  E+ + S +SE+  C SS+KR + E   G+F        DLNLPA+ VD
Sbjct: 541 LDSGTSGVTSRPEQASGSRSSEDTICSSSQKRPKKE---GSFLNNKVTLLDLNLPADFVD 597

BLAST of Lsi10G003400 vs. TAIR 10
Match: AT4G34370.2 (RING/U-box superfamily protein )

HSP 1 Score: 813.1 bits (2099), Expect = 1.7e-235
Identity = 407/602 (67.61%), Postives = 492/602 (81.73%), Query Frame = 0

Query: 131 MDDCISSDED--YYDSDRDSLHGLENEDSEIQWV-PKGPATKVITKESLLAAQKEDMRRV 190
           MDD  S++E+  YY SD+DSL G++NE+SE+Q +  K   T+VIT+ESLLAAQ+ED+ RV
Sbjct: 1   MDDYFSAEEEACYYSSDQDSLDGIDNEESELQPLSSKRSNTQVITQESLLAAQREDLLRV 60

Query: 191 MDLLSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNSSSEP-SSM 250
           M+LLS++E+HARTLLIHY+WDVEKLFAV VEKGK  LF+ AGVT+ + +  +SS P SS 
Sbjct: 61  MELLSIKEHHARTLLIHYQWDVEKLFAVFVEKGKDSLFSGAGVTVFDYQYGNSSFPQSSQ 120

Query: 251 VMCDICMEEVHDYDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAV 310
           + CD+CME++                  WTEHF V+INEGQS+RIRCMAH+CNAICDE +
Sbjct: 121 MSCDVCMEDLPG----------------WTEHFTVQINEGQSKRIRCMAHQCNAICDEDI 180

Query: 311 VRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDDEFCEVECSCGI 370
           VR+LVSK+ PDLA KFDR+LLESYIEDN+ VKWCPSTPHCGNAIR EDD+ CEVECSCG+
Sbjct: 181 VRSLVSKKRPDLAAKFDRYLLESYIEDNRMVKWCPSTPHCGNAIRAEDDKLCEVECSCGL 240

Query: 371 QFCFSCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNLVS 430
           QFCFSCL +AHSPCSCLMWELW KKCRDESET+NWITVHTK CPKC+KPVEKNGGCNLV 
Sbjct: 241 QFCFSCLCQAHSPCSCLMWELWRKKCRDESETINWITVHTKLCPKCYKPVEKNGGCNLVR 300

Query: 431 CICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYKAH 490
           CICGQ FCWLCGGATG +HT+ SI+GHSCGRY+++ E++ ERAKRDL RY HYH+RYKAH
Sbjct: 301 CICGQCFCWLCGGATGSDHTYRSIAGHSCGRYQDDKEKQMERAKRDLNRYTHYHHRYKAH 360

Query: 491 TDSFKLESKLKESIQEKISISEERESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAFYMFG 550
           TDS KLE KL+++I EK+S SE+RE  L+DFSWV NGL RLFRSRRVLSYSY FA+YMFG
Sbjct: 361 TDSSKLEDKLRDTIHEKVSKSEKRELKLKDFSWVTNGLDRLFRSRRVLSYSYAFAYYMFG 420

Query: 551 DELFKDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFEQYTKDKVMEIRMQVINL 610
           +E+FKDEMT  EREIK++LFEDQQQQLE+NVEKLS+FLEEPF++++ DKVM IR+Q+INL
Sbjct: 421 EEMFKDEMTPEEREIKKNLFEDQQQQLESNVEKLSQFLEEPFDEFSNDKVMAIRIQIINL 480

Query: 611 SVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSDKYPP 670
           SV  DTLCKKMY+CIENDLLGSL+LGIHNI+PY+SKGIE+A +  A WNSK   +DK+ P
Sbjct: 481 SVAVDTLCKKMYECIENDLLGSLQLGIHNISPYRSKGIEQAAQFYASWNSK--DADKFQP 540

Query: 671 SDCGTSGGLSECERLT-SLNSEENGC-SSRKRARAEVVTGNF-------FDLNLPAEVVD 720
            D GTSG  S  E+ + S +SE+  C SS+KR + E   G+F        DLNLPA+ VD
Sbjct: 541 LDSGTSGVTSRPEQASGSRSSEDTICSSSQKRPKKE---GSFLNNKVTLLDLNLPADFVD 581

BLAST of Lsi10G003400 vs. TAIR 10
Match: AT3G27710.1 (RING/U-box superfamily protein )

HSP 1 Score: 644.4 bits (1661), Expect = 1.1e-184
Identity = 322/551 (58.44%), Postives = 419/551 (76.04%), Query Frame = 0

Query: 132 DDCISSDEDYYDSDRDSLHGLEN-EDSEIQWVP---KGPATKVITKESLLAAQKEDMRRV 191
           DD +  D+DY + + ++    +N  ++E+   P       ++VI KESL+AAQKE + RV
Sbjct: 3   DDYMMLDDDYGEEEDENYSEDDNYSEAEVDLQPVTSTKSTSQVIKKESLVAAQKEILVRV 62

Query: 192 MDLLSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATAGVTMIENRSNSSSEPSSMV 251
           M+LLS++E  ARTLLI+Y+W+VEKLF+V  ++GK  +F+ AG+T+      +S +    +
Sbjct: 63  MELLSVKENQARTLLIYYQWNVEKLFSVFADQGKDRMFSCAGLTVFVPSLVTSKK---TM 122

Query: 252 MCDICME-EVHDYDATRVDCGHCFCNNCWTEHFIVKINEGQSRRIRCMAHKCNAICDEAV 311
            CD+CME ++     TR++CGH FCN+CW  HF VKINEG+S+RI CMAH+C AICDE V
Sbjct: 123 KCDVCMEDDLPSNVMTRMECGHRFCNDCWIGHFTVKINEGESKRILCMAHECKAICDEDV 182

Query: 312 VRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGNAIRVEDD--EFCEVECSC 371
           VR LVS   P+LA+++DRFL+ESY+EDN  VKWCPS PHCG+AIR  +D  +  EV CSC
Sbjct: 183 VRKLVS---PELADRYDRFLIESYVEDNNMVKWCPSKPHCGSAIRKIEDGHDVVEVGCSC 242

Query: 372 GIQFCFSCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHTKPCPKCHKPVEKNGGCNL 431
           G+QFCFSCLSE+HSPCSCLMW+LW KKC DESETVNWITV+TK CPKC KP++K  GCNL
Sbjct: 243 GLQFCFSCLSESHSPCSCLMWKLWKKKCEDESETVNWITVNTKLCPKCSKPIQKRDGCNL 302

Query: 432 VSCICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKAERAKRDLYRYMHYHNRYK 491
           ++C CGQ FCWLCG ATGR+HT++SI+GHSCGRYK+E  ++ ERA+RDL RY HYH RYK
Sbjct: 303 MTCKCGQHFCWLCGQATGRDHTYTSIAGHSCGRYKDEKVRQLERAQRDLDRYTHYHYRYK 362

Query: 492 AHTDSFKLESKLKESIQEK-ISISEER-ESMLRDFSWVNNGLSRLFRSRRVLSYSYPFAF 551
           AH DS KLE KL++SI EK +S SE + + + +++SWV + ++RLF SRR+LS SYPFAF
Sbjct: 363 AHIDSLKLEDKLRKSILEKAVSNSETKDQKVFKEYSWVTDAVNRLFISRRILSQSYPFAF 422

Query: 552 YMFGDELFKDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFLEEPFEQYTKDKVMEIRMQ 611
           YMFG+ELFKDEM+E EREIK++LFEDQQQQLE NVEKLSK LEEPF++Y  +KV+E+   
Sbjct: 423 YMFGEELFKDEMSEKEREIKKNLFEDQQQQLEGNVEKLSKILEEPFDEYDHEKVVEMMRH 482

Query: 612 VINLSVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGIEKALELSACWNSKANTSD 671
           + NL+ + D LCK+MY+CIEN+LLG ++ G HNIAPY+SKGIE+A E  A          
Sbjct: 483 LTNLTAVVDNLCKEMYECIENELLGPIQFGNHNIAPYRSKGIEQATEFCA---------- 535

Query: 672 KYPPSDCGTSG 674
               SDCG+SG
Sbjct: 543 --ESSDCGSSG 535

BLAST of Lsi10G003400 vs. TAIR 10
Match: AT3G27720.1 (IBR domain containing protein )

HSP 1 Score: 514.6 bits (1324), Expect = 1.3e-145
Identity = 267/488 (54.71%), Postives = 339/488 (69.47%), Query Frame = 0

Query: 173 KESLLAAQKEDMRRVMDLLSLREYHARTLLIHYRWDVEKLFAVLVEKGKSHLFATA---- 232
           +ES L   +E   +VM+ LS+ E  ARTLLI Y+W+V+KLF+V  ++GK  LF+ A    
Sbjct: 31  EESDLQHSREPTSQVMEFLSVTENQARTLLIQYQWNVDKLFSVYTDQGKDVLFSRAEEEE 90

Query: 233 GVTMIENRSNSSSEPSSMVMCDICMEEVHDYDATRVDCGHCFCNNCWTEHFIVKINEGQS 292
           G    E       E       +   E   D +A               E  +    E + 
Sbjct: 91  GEDKEEEEGGEDEEEEEGGEDEEAEEGGEDEEA---------------EEGVEDEEEEED 150

Query: 293 RRIRCMAHKCNAICDEAVVRNLVSKRHPDLANKFDRFLLESYIEDNKRVKWCPSTPHCGN 352
            +            D+ V   LVS    +LA KFDRFL+ESY+EDN  VKWCPSTPHCGN
Sbjct: 151 EK------------DDEV--QLVS---TELAEKFDRFLIESYVEDNNMVKWCPSTPHCGN 210

Query: 353 AIR--VEDDEFCEVECSCGIQFCFSCLSEAHSPCSCLMWELWIKKCRDESETVNWITVHT 412
           AIR   +D +  EVECSCG+QFCFSCLSE+HSPCSCLMW+LW KKC DESETVNW+TV+T
Sbjct: 211 AIRNIKDDGDVDEVECSCGLQFCFSCLSESHSPCSCLMWKLWKKKCEDESETVNWMTVNT 270

Query: 413 KPCPKCHKPVEKNGGCNLVSCICGQAFCWLCGGATGREHTWSSISGHSCGRYKEESEQKA 472
           K CPKC KP++K  GCN ++C CGQ FCWLCG ATGR+H++SSI+GHSCGRYKEE  ++ 
Sbjct: 271 KLCPKCSKPIQKRDGCNHMTCKCGQHFCWLCGQATGRDHSYSSIAGHSCGRYKEEKVRQL 330

Query: 473 ERAKRDLYRYMHYHNRYKAHTDSFKLESKLKESIQEKISISEER--ESMLRDFSWVNNGL 532
           ERA+RDL RY HYH RYKAH DS KLE KLK+SI +K  ++ E   + + +++SW+ + +
Sbjct: 331 ERAQRDLDRYTHYHYRYKAHIDSLKLEDKLKKSILKKAVLNSETKDQKVFKEYSWIIDAV 390

Query: 533 SRLFRSRRVLSYSYPFAFYMFGDELFKDEMTEAEREIKQHLFEDQQQQLEANVEKLSKFL 592
           +RLFRSRR+LSYSYPF FYMFG ELFKD+M++ ER IK++LFEDQQQQLE NVE+LSK L
Sbjct: 391 NRLFRSRRILSYSYPFVFYMFGKELFKDDMSDEERNIKKNLFEDQQQQLEGNVERLSKIL 450

Query: 593 EEPFEQYTKDKVMEIRMQVINLSVITDTLCKKMYDCIENDLLGSLELGIHNIAPYKSKGI 652
           EEPF++Y  +KV+E+   + NL+ + D LCK+MY+CIEN+LLG L  GIHNIAPY+SKGI
Sbjct: 451 EEPFDEYDHEKVVEMMRHLTNLTAVVDNLCKEMYECIENELLGPLISGIHNIAPYRSKGI 486

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84RR21.3e-24871.36Probable E3 ubiquitin-protein ligase ARI2 OS=Arabidopsis thaliana OX=3702 GN=ARI... [more]
Q949V61.6e-24669.60Probable E3 ubiquitin-protein ligase ARI1 OS=Arabidopsis thaliana OX=3702 GN=ARI... [more]
Q9LVX01.5e-18358.44Probable E3 ubiquitin-protein ligase ARI3 OS=Arabidopsis thaliana OX=3702 GN=ARI... [more]
Q9LVW97.4e-18360.27Putative E3 ubiquitin-protein ligase ARI4 OS=Arabidopsis thaliana OX=3702 GN=ARI... [more]
Q949811.0e-6232.59E3 ubiquitin-protein ligase ariadne-1 OS=Drosophila melanogaster OX=7227 GN=ari-... [more]
Match NameE-valueIdentityDescription
A0A5D3CP890.0e+0098.30Putative E3 ubiquitin-protein ligase ARI2 OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3C9A10.0e+0098.30probable E3 ubiquitin-protein ligase ARI2 OS=Cucumis melo OX=3656 GN=LOC10349843... [more]
A0A0A0LL320.0e+0097.96Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G238850 PE=3 SV=1[more]
A0A6J1J7N90.0e+0094.74probable E3 ubiquitin-protein ligase ARI2 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1FVJ50.0e+0094.74probable E3 ubiquitin-protein ligase ARI2 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
XP_038901258.10.0e+0098.30probable E3 ubiquitin-protein ligase ARI2 isoform X2 [Benincasa hispida][more]
XP_038901257.10.0e+0097.64probable E3 ubiquitin-protein ligase ARI2 isoform X1 [Benincasa hispida][more]
XP_008459262.10.0e+0098.30PREDICTED: probable E3 ubiquitin-protein ligase ARI2 [Cucumis melo] >KAA0045954.... [more]
XP_004153333.10.0e+0097.96probable E3 ubiquitin-protein ligase ARI2 isoform X1 [Cucumis sativus] >KGN61769... [more]
XP_031736976.10.0e+0097.45probable E3 ubiquitin-protein ligase ARI2 isoform X2 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT2G16090.19.4e-25071.36RING/U-box superfamily protein [more]
AT4G34370.11.2e-24769.60RING/U-box superfamily protein [more]
AT4G34370.21.7e-23567.61RING/U-box superfamily protein [more]
AT3G27710.11.1e-18458.44RING/U-box superfamily protein [more]
AT3G27720.11.3e-14554.71IBR domain containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002867IBR domainSMARTSM00647ibrneu5coord: 390..455
e-value: 0.012
score: 23.3
coord: 320..382
e-value: 3.6E-20
score: 83.0
IPR002867IBR domainPFAMPF01485IBRcoord: 398..440
e-value: 1.4E-5
score: 25.2
coord: 321..382
e-value: 2.2E-12
score: 47.0
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 243..334
e-value: 1.1E-17
score: 65.7
NoneNo IPR availableGENE3D1.20.120.1750coord: 378..626
e-value: 1.1E-63
score: 217.4
NoneNo IPR availablePANTHERPTHR11685:SF345RBR-TYPE E3 UBIQUITIN TRANSFERASEcoord: 135..645
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 244..332
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 321..389
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 390..447
IPR031127E3 ubiquitin ligase RBR familyPANTHERPTHR11685RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAININGcoord: 135..645
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 249..297
score: 9.774726
IPR044066TRIAD supradomainPROSITEPS51873TRIADcoord: 245..459
score: 45.358398

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G003400.1Lsi10G003400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0000151 ubiquitin ligase complex
molecular_function GO:0046872 metal ion binding
molecular_function GO:0031624 ubiquitin conjugating enzyme binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0004842 ubiquitin-protein transferase activity