Homology
BLAST of Lsi10G003310 vs. ExPASy Swiss-Prot
Match:
M4ISH1 (Probable CoA ligase CCL6 OS=Humulus lupulus OX=3486 GN=CCL6 PE=2 SV=1)
HSP 1 Score: 966.1 bits (2496), Expect = 2.5e-280
Identity = 480/720 (66.67%), Postives = 556/720 (77.22%), Query Frame = 0
Query: 6 TYTVAVEAATPESGGRPSAGPIYRSIYAKDGLMQLPDGLESPWQFFRFVFFGHFFLLKKG 65
+YTV VE A P + PSAGP+YRSIYA+DGL++LP+GL+SPW+F
Sbjct: 2 SYTVKVEEARPATEKMPSAGPVYRSIYARDGLLELPEGLQSPWEF--------------- 61
Query: 66 TIASHVYVIRLLLFVSNLISIATTVVPCGSFDLKSSNLSLIGGGELNSKSRRVNDSVKRN 125
++ SVKR+
Sbjct: 62 ----------------------------------------------------LSGSVKRS 121
Query: 126 PGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNCPEW 185
P MLGRRQ DS+AG YVWLTYQE +D AIRM SA+R RGVN GDRCGIYG+NCP+W
Sbjct: 122 PKTPMLGRRQIK-DSEAGPYVWLTYQEVHDEAIRMASAMRSRGVNPGDRCGIYGTNCPQW 181
Query: 186 IISMEVCNFVFLRMYMRPVIAKLSLMYLCANAVEFIINHAEVSIAFVQENKLASIFSCLP 245
I++M+ C Y + L NAVEFIINH EVSIAFVQENK+++I SCLP
Sbjct: 182 IVAMQAC-------YSHAITYVPLYDTLGPNAVEFIINHGEVSIAFVQENKISAILSCLP 241
Query: 246 RCSNQLKTIVSFTNVSSVHKKEAEELGVSFFSWEEFYQLVQYGSLDCELPTKQKTNICTI 305
CS+ LKTIVSF N++SV KKE E LGVS FSWEEF QL G+L ELP K +T+ICT+
Sbjct: 242 NCSSLLKTIVSFGNITSVQKKETEALGVSCFSWEEFSQL---GNLSGELPEKHRTDICTL 301
Query: 306 MYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPLSHVYDQIMET 365
MYTSG TGEPKGVIL N AIMAE+LS D++L LTDKV +EED YFS+LPL+HVYDQI+E
Sbjct: 302 MYTSGATGEPKGVILTNEAIMAEILSTDNMLELTDKVFSEEDVYFSYLPLAHVYDQIVEN 361
Query: 366 YCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLF 425
YCIYKGS+IG+WRGDIRFL+DD+QELKPTIF GVPRVYDRIY+GI +KVSSGG+L+KMLF
Sbjct: 362 YCIYKGSAIGYWRGDIRFLMDDLQELKPTIFCGVPRVYDRIYAGIFHKVSSGGTLKKMLF 421
Query: 426 DYAYNYKLGSLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPKHVEEFLRV 485
YAYNYK+ +++KGL +AAPL+DKL FDKIK+ FGGR+RL SGAAPLP HVEE+LRV
Sbjct: 422 QYAYNYKMANMEKGLPHGQAAPLMDKLFFDKIKQGFGGRIRLMFSGAAPLPHHVEEYLRV 481
Query: 486 TSCATLSQGYGLTESCGGCFTSIANVFSMMGTVGVPVTTIEARLESVPDMGYDALTGTPR 545
TSCA LSQGYGLTESCGGC TSIAN+F M+GTVGVP+TTIEARLESVP+MGYDAL+ PR
Sbjct: 482 TSCAALSQGYGLTESCGGCLTSIANIFPMIGTVGVPMTTIEARLESVPEMGYDALSDKPR 541
Query: 546 GEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGE 605
GEICLRG+TLFSGYHKR+DLT+DVL+DGWFHTGDIGEWQP+G+MKIIDRKKNIFKLSQGE
Sbjct: 542 GEICLRGTTLFSGYHKREDLTKDVLVDGWFHTGDIGEWQPNGAMKIIDRKKNIFKLSQGE 601
Query: 606 YVAVENIENIFLQCPLITSIWVYGNSFESFLVGVVVPDQKALEDWARNHNVTGDFKSLCE 665
YV VENIE I+LQCPLI SIWVYGNSFESFLV VVVP++ ALE+WA N N+T DFKSLCE
Sbjct: 602 YVPVENIEGIYLQCPLIASIWVYGNSFESFLVAVVVPERLALENWAANRNLTDDFKSLCE 643
Query: 666 NLEARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYK 725
N +A KYILDELNS +KH LRGFE+LKA++LEP PFDMERDLITPTFKLKRPQLLKYYK
Sbjct: 662 NPKASKYILDELNSTAKKHQLRGFEMLKAVYLEPNPFDMERDLITPTFKLKRPQLLKYYK 643
BLAST of Lsi10G003310 vs. ExPASy Swiss-Prot
Match:
Q9XIA9 (Long chain acyl-CoA synthetase 2 OS=Arabidopsis thaliana OX=3702 GN=LACS2 PE=2 SV=1)
HSP 1 Score: 858.6 bits (2217), Expect = 5.7e-248
Identity = 432/729 (59.26%), Postives = 530/729 (72.70%), Query Frame = 0
Query: 1 MTMPTTYTVAVEAATPESGGRPSAGPIYRSIYAKDGLMQLPDGLESPWQFFRFVFFGHFF 60
M++ + VE P + PSAGP+YR YAKDGL+ LP ++SPWQFF
Sbjct: 1 MSLAADNVLLVEEGRPATAEHPSAGPVYRCKYAKDGLLDLPTDIDSPWQFF--------- 60
Query: 61 LLKKGTIASHVYVIRLLLFVSNLISIATTVVPCGSFDLKSSNLSLIGGGELNSKSRRVND 120
++
Sbjct: 61 ----------------------------------------------------------SE 120
Query: 121 SVKRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGS 180
+VK+ P +MLG+R T DSK G Y W+TY+EA+DAAIR+GSAIR RGV+ G CGIYG+
Sbjct: 121 AVKKYPNEQMLGQRVTT-DSKVGPYTWITYKEAHDAAIRIGSAIRSRGVDPGHCCGIYGA 180
Query: 181 NCPEWIISMEVCNFVFLRMYMRPVIAKLSLM-YLCANAVEFIINHAEVSIAFVQENKLAS 240
NCPEWII+ME C M I + L L NAVEFIINHAEVS+ FVQE ++S
Sbjct: 181 NCPEWIIAMEAC--------MSQGITYVPLYDSLGVNAVEFIINHAEVSLVFVQEKTVSS 240
Query: 241 IFSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSFFSWEEFYQLVQYGSLD-CELPTKQ 300
I SC CS+ LKTIVSF VSS K+EA+ VS FSW EF + G+LD LP K+
Sbjct: 241 ILSCQKGCSSNLKTIVSFGEVSSTQKEEAKNQCVSLFSWNEFSLM---GNLDEANLPRKR 300
Query: 301 KTNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPLSHV 360
KT+ICTIMYTSGTTGEPKGVILNNAAI +VLS+D +L +TD+ D +FS+LPL+H
Sbjct: 301 KTDICTIMYTSGTTGEPKGVILNNAAISVQVLSIDKMLEVTDRSCDTSDVFFSYLPLAHC 360
Query: 361 YDQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGG 420
YDQ+ME Y + +GSS+G+WRGDIR+L+DD+Q LKPT+F GVPRVYD++Y+GI+ K+S+ G
Sbjct: 361 YDQVMEIYFLSRGSSVGYWRGDIRYLMDDVQALKPTVFCGVPRVYDKLYAGIMQKISASG 420
Query: 421 SLRKMLFDYAYNYKLGSLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPKH 480
+RK LFD+AYNYKLG+++KG QE+A+P LD+L+FDKIKEA GGR + LSGAAPLP+H
Sbjct: 421 LIRKKLFDFAYNYKLGNMRKGFSQEEASPRLDRLMFDKIKEALGGRAHMLLSGAAPLPRH 480
Query: 481 VEEFLRVTSCATLSQGYGLTESCGGCFTSIANVFSMMGTVGVPVTTIEARLESVPDMGYD 540
VEEFLR+ + LSQGYGLTESCGG FT++A VFSM+GTVGVP+ T+EARL SVP+MGYD
Sbjct: 481 VEEFLRIIPASNLSQGYGLTESCGGSFTTLAGVFSMVGTVGVPMPTVEARLVSVPEMGYD 540
Query: 541 ALTG-TPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKN 600
A + PRGEICLRG+++FSGYHKRQDLT+ VLIDGWFHTGDIGEWQ DGSMKIIDRKKN
Sbjct: 541 AFSADVPRGEICLRGNSMFSGYHKRQDLTDQVLIDGWFHTGDIGEWQEDGSMKIIDRKKN 600
Query: 601 IFKLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVVVPDQKALEDWAR-NHNV 660
IFKLSQGEYVAVEN+EN + +CPLI IWVYGNSFESFLVGVVVPD+KA+EDWA+ N+
Sbjct: 601 IFKLSQGEYVAVENLENTYSRCPLIAQIWVYGNSFESFLVGVVVPDRKAIEDWAKLNYQS 650
Query: 661 TGDFKSLCENLEARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLK 720
DF+SLC+NL+A+KY LDELNS +++ L+GFE+LKAIHLEP PFD+ERDLITPTFKLK
Sbjct: 661 PNDFESLCQNLKAQKYFLDELNSTAKQYQLKGFEMLKAIHLEPNPFDIERDLITPTFKLK 650
Query: 721 RPQLLKYYK 726
RPQLL++YK
Sbjct: 721 RPQLLQHYK 650
BLAST of Lsi10G003310 vs. ExPASy Swiss-Prot
Match:
Q9C7W4 (Long chain acyl-CoA synthetase 3 OS=Arabidopsis thaliana OX=3702 GN=LACS3 PE=2 SV=1)
HSP 1 Score: 731.9 bits (1888), Expect = 8.1e-210
Identity = 364/720 (50.56%), Postives = 479/720 (66.53%), Query Frame = 0
Query: 7 YTVAVEAATP-ESGGRPSAGPIYRSIYAKDGLMQLPDGLESPWQFFRFVFFGHFFLLKKG 66
Y V VE GG PS GP+YRSIYAKDG + PD L S W FR
Sbjct: 6 YIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRL------------ 65
Query: 67 TIASHVYVIRLLLFVSNLISIATTVVPCGSFDLKSSNLSLIGGGELNSKSRRVNDSVKRN 126
SV+++
Sbjct: 66 -------------------------------------------------------SVEKS 125
Query: 127 PGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNCPEW 186
P N MLGRR+ D KAG YVW TY+E ++ I++G++IR GV GD+CGIYG+N PEW
Sbjct: 126 PNNPMLGRREI-VDGKAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEW 185
Query: 187 IISMEVCNFVFLRMYMRPVIAKLSLMYLCANAVEFIINHAEVSIAFVQENKLASIFSCLP 246
IISME CN L Y P+ L A A+EFII HAEVS+AF +ENK++ + P
Sbjct: 186 IISMEACNAHGL--YCVPLYDTLG-----AGAIEFIICHAEVSLAFAEENKISELLKTAP 245
Query: 247 RCSNQLKTIVSFTNVSSVHKKEAEELGVSFFSWEEFYQLVQYGSLDCELPTKQKTNICTI 306
+ + LK IVSF V++ + EAE ++ +SW++F +L + ELP K+++++CTI
Sbjct: 246 KSTKYLKYIVSFGEVTNNQRVEAERHRLTIYSWDQFLKLGE--GKHYELPEKRRSDVCTI 305
Query: 307 MYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPLSHVYDQIMET 366
MYTSGTTG+PKGV+L N +I+ + V LL D+ T +D Y S+LPL+H++D+++E
Sbjct: 306 MYTSGTTGDPKGVLLTNESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEE 365
Query: 367 YCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLF 426
CIY+ +SIGFWRGD++ L++DI LKPT+F VPRV +RIY+G+ K+S GG ++K LF
Sbjct: 366 LCIYEAASIGFWRGDVKILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLF 425
Query: 427 DYAYNYKLGSLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPKHVEEFLRV 486
++A+ YK +++KG E+A+P+ DK+VF K+KE GG VRL LSGAAPL H+E FLRV
Sbjct: 426 NFAFKYKHKNMEKGQPHEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRV 485
Query: 487 TSCATLSQGYGLTESCGGCFTSIANVFSMMGTVGVPVTTIEARLESVPDMGYDALTGTPR 546
+CA + QGYGLTESCGG F SI N SM+GTVG PV ++ RLESVP+MGYDAL PR
Sbjct: 486 VACAHVLQGYGLTESCGGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPR 545
Query: 547 GEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGE 606
GEIC+RG TLFSGY+KR+DLT++V IDGW HTGD+GEWQPDG+MKIIDRKKNIFKLSQGE
Sbjct: 546 GEICIRGKTLFSGYYKREDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGE 605
Query: 607 YVAVENIENIFLQCPLITSIWVYGNSFESFLVGVVVPDQKALEDWARNHNVTGDFKSLCE 666
YVAVEN+ENI+ I SIWVYGNS+ES+LV VV P + +E WA+ H V+GDF+S+C
Sbjct: 606 YVAVENLENIYSHVAAIESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICR 648
Query: 667 NLEARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYK 726
N + ++++L E N + L+GFEL+K +HL+ PFDMERDLITP++K+KRPQLLKYY+
Sbjct: 666 NQKTKEFVLGEFNRVAKDKKLKGFELIKGVHLDTVPFDMERDLITPSYKMKRPQLLKYYQ 648
BLAST of Lsi10G003310 vs. ExPASy Swiss-Prot
Match:
Q9T0A0 (Long chain acyl-CoA synthetase 4 OS=Arabidopsis thaliana OX=3702 GN=LACS4 PE=2 SV=1)
HSP 1 Score: 728.4 bits (1879), Expect = 8.9e-209
Identity = 364/728 (50.00%), Postives = 480/728 (65.93%), Query Frame = 0
Query: 1 MTMPTTYTVAVEAATPESGGRPSAGPIYRSIYAKDGLMQLPDGLESPWQFFRFVFFGHFF 60
M+ Y VE S GRPS GP+YRSI+AKDG +G++S W FR
Sbjct: 1 MSQQKKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRM------- 60
Query: 61 LLKKGTIASHVYVIRLLLFVSNLISIATTVVPCGSFDLKSSNLSLIGGGELNSKSRRVND 120
Sbjct: 61 ------------------------------------------------------------ 120
Query: 121 SVKRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGS 180
SV++ P N MLGRR+ D K G YVW TYQE YD +++G+++R GV +CGIYG+
Sbjct: 121 SVEKYPNNPMLGRREI-VDGKPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGA 180
Query: 181 NCPEWIISMEVCNFVFLRMYMRPVIAKLSLMYLCANAVEFIINHAEVSIAFVQENKLASI 240
N PEWIISME CN L Y P+ L A+AVEFII+H+EVSI FV+E K++ +
Sbjct: 181 NSPEWIISMEACNAHGL--YCVPLYDTLG-----ADAVEFIISHSEVSIVFVEEKKISEL 240
Query: 241 FSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSFFSWEEFYQLVQYGSLDCELPTKQKT 300
F P + +KT+VSF VS K+EAE G+ ++W+EF +L + D LP K+K+
Sbjct: 241 FKTCPNSTEYMKTVVSFGGVSREQKEEAETFGLVIYAWDEFLKLGEGKQYD--LPIKKKS 300
Query: 301 NICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPLSHVYD 360
+ICTIMYTSGTTG+PKGV+++N +I+ + V LL ++ T +D Y S+LPL+H++D
Sbjct: 301 DICTIMYTSGTTGDPKGVMISNESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFD 360
Query: 361 QIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSL 420
+++E I G++IGFWRGD++ L++D+ ELKPTIF VPRV DR+YSG+ K+S GG L
Sbjct: 361 RVIEECFIQHGAAIGFWRGDVKLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFL 420
Query: 421 RKMLFDYAYNYKLGSLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPKHVE 480
+K +FD A++YK G +KKG +A+PL DKLVF K+K+ GG VR+ LSGAAPL HVE
Sbjct: 421 KKFIFDSAFSYKFGYMKKGQSHVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVE 480
Query: 481 EFLRVTSCATLSQGYGLTESCGGCFTSIANVFSMMGTVGVPVTTIEARLESVPDMGYDAL 540
FLRV +C + QGYGLTESC G F S+ + M+GTVG PV ++ RLESVP+M YDAL
Sbjct: 481 SFLRVVACCHVLQGYGLTESCAGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDAL 540
Query: 541 TGTPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFK 600
T RGEIC+RG TLFSGY+KR+DLT++VLIDGW HTGD+GEWQPDGSMKIIDRKKNIFK
Sbjct: 541 ASTARGEICIRGKTLFSGYYKREDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFK 600
Query: 601 LSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVVVPDQKALEDWARNHNVTGDF 660
LSQGEYVAVENIENI+ + + S+WVYGNSFESFL+ + P+Q LE WA + V+GD+
Sbjct: 601 LSQGEYVAVENIENIYGEVQAVDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDY 651
Query: 661 KSLCENLEARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQL 720
+LC+N +A+++IL EL ++ ++GFE++KAIHL+P PFDMERDL+TPTFK KRPQL
Sbjct: 661 DALCQNEKAKEFILGELVKMAKEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQL 651
Query: 721 LKYYKAAL 729
LKYY++ +
Sbjct: 721 LKYYQSVI 651
BLAST of Lsi10G003310 vs. ExPASy Swiss-Prot
Match:
Q9T009 (Long chain acyl-CoA synthetase 5 OS=Arabidopsis thaliana OX=3702 GN=LACS5 PE=2 SV=1)
HSP 1 Score: 719.2 bits (1855), Expect = 5.4e-206
Identity = 359/725 (49.52%), Postives = 473/725 (65.24%), Query Frame = 0
Query: 1 MTMPTTYTVAVEAATPESGGRPSAGPIYRSIYAKDGLMQLPDGLESPWQFFRFVFFGHFF 60
MT + VEAA + G PS GP+YRS +A++G DG++S W FR
Sbjct: 1 MTSQKRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFR-------- 60
Query: 61 LLKKGTIASHVYVIRLLLFVSNLISIATTVVPCGSFDLKSSNLSLIGGGELNSKSRRVND 120
Sbjct: 61 -----------------------------------------------------------T 120
Query: 121 SVKRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGS 180
+V++ P NRMLGRR+ + + KAG YVW TY+E YD I++G+++R G+ G++CGIYG
Sbjct: 121 AVEKYPNNRMLGRREIS-NGKAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGI 180
Query: 181 NCPEWIISMEVCNFVFLRMYMRPVIAKLSLMYLCANAVEFIINHAEVSIAFVQENKLASI 240
NC EWIISME CN L Y P+ L A AVEFII+HAEVSIAFV+E K+ +
Sbjct: 181 NCCEWIISMEACNAHGL--YCVPLYDTLG-----AGAVEFIISHAEVSIAFVEEKKIPEL 240
Query: 241 FSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSFFSWEEFYQLVQYGSLDCELPTKQKT 300
F P + +KT+VSF V K+EAE+LG+ SW+EF +L + ELP K+ +
Sbjct: 241 FKTCPNSTKYMKTVVSFGGVKPEQKEEAEKLGLVIHSWDEFLKLGE--GKQYELPIKKPS 300
Query: 301 NICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPLSHVYD 360
+ICTIMYTSGTTG+PKGV+++N +I+ V H L + +E+D Y S+LPL+HV+D
Sbjct: 301 DICTIMYTSGTTGDPKGVMISNESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFD 360
Query: 361 QIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSL 420
+ +E I G SIGFWRGD++ L++D+ ELKP+IF VPRV DR+Y+G+ K+S GG
Sbjct: 361 RAIEECIIQVGGSIGFWRGDVKLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFF 420
Query: 421 RKMLFDYAYNYKLGSLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPKHVE 480
+K +FD A++YK G++KKG A+P DKLVF+K+K+ GG VR+ LSGAAPL H+E
Sbjct: 421 KKKVFDVAFSYKFGNMKKGQSHVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIE 480
Query: 481 EFLRVTSCATLSQGYGLTESCGGCFTSIANVFSMMGTVGVPVTTIEARLESVPDMGYDAL 540
FLRV +C + QGYGLTESC G F + + M+GTVG PV ++ RLESVP+M YDAL
Sbjct: 481 SFLRVVACCNVLQGYGLTESCAGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDAL 540
Query: 541 TGTPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFK 600
TPRGEIC+RG TLFSGY+KR+DLT++V IDGW HTGD+GEWQP+GSMKIIDRKKNIFK
Sbjct: 541 GSTPRGEICIRGKTLFSGYYKREDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFK 600
Query: 601 LSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVVVPDQKALEDWARNHNVTGDF 660
L+QGEYVAVEN+EN++ Q +I SIWVYGNSFESFLV + P Q+ LE WA + V GDF
Sbjct: 601 LAQGEYVAVENLENVYSQVEVIESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDF 648
Query: 661 KSLCENLEARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQL 720
S+C+N +A+ +IL EL +++ L+GFE++K +HLEP FDMERDL+TPT+K KRPQL
Sbjct: 661 NSICQNAKAKAFILGELVKTAKENKLKGFEIIKDVHLEPVAFDMERDLLTPTYKKKRPQL 648
Query: 721 LKYYK 726
LKYY+
Sbjct: 721 LKYYQ 648
BLAST of Lsi10G003310 vs. ExPASy TrEMBL
Match:
A0A5A7TX35 (Long chain acyl-CoA synthetase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G005130 PE=4 SV=1)
HSP 1 Score: 1198.0 bits (3098), Expect = 0.0e+00
Identity = 607/723 (83.96%), Postives = 622/723 (86.03%), Query Frame = 0
Query: 3 MPTTYTVAVEAATPESGGRPSAGPIYRSIYAKDGLMQLPDGLESPWQFFRFVFFGHFFLL 62
M T YTV VEA TPE+GGRPSAGP+YRSIYAKDGL+QLPD LESPWQFF
Sbjct: 1 MSTPYTVVVEAETPETGGRPSAGPVYRSIYAKDGLLQLPDELESPWQFF----------- 60
Query: 63 KKGTIASHVYVIRLLLFVSNLISIATTVVPCGSFDLKSSNLSLIGGGELNSKSRRVNDSV 122
+DSV
Sbjct: 61 --------------------------------------------------------SDSV 120
Query: 123 KRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNC 182
KRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNC
Sbjct: 121 KRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNC 180
Query: 183 PEWIISMEVCNFVFLRMYMRPVIAKLSLMYLCANAVEFIINHAEVSIAFVQENKLASIFS 242
PEWIISMEVCNFV +A ANAVEFI+NHAEVSIAFVQENKLASIFS
Sbjct: 181 PEWIISMEVCNFVLSSCIDNQTVAG-------ANAVEFIVNHAEVSIAFVQENKLASIFS 240
Query: 243 CLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSFFSWEEFYQLVQYGSLDCELPTKQKTNI 302
CLPRCSNQLKTIVSFTNVSSVHKKEAEELGVS FSWEEFYQL GSLDCELP+KQKTNI
Sbjct: 241 CLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSCFSWEEFYQL---GSLDCELPSKQKTNI 300
Query: 303 CTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPLSHVYDQI 362
CTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKV TEEDSYFSFLPL+HVYDQI
Sbjct: 301 CTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVATEEDSYFSFLPLAHVYDQI 360
Query: 363 METYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRK 422
METYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRK
Sbjct: 361 METYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRK 420
Query: 423 MLFDYAYNYKLGSLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPKHVEEF 482
MLFDYAY YKLG LKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLP+ VEEF
Sbjct: 421 MLFDYAYTYKLGYLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRLVEEF 480
Query: 483 LRVTSCATLSQGYGLTESCGGCFTSIANVFSMMGTVGVPVTTIEARLESVPDMGYDALTG 542
LRVTSCATLSQGYGLTESCGGCFTSIANVF+M+GTVGVPVTTIEARLESVPDMGYDAL
Sbjct: 481 LRVTSCATLSQGYGLTESCGGCFTSIANVFTMVGTVGVPVTTIEARLESVPDMGYDALAT 540
Query: 543 TPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLS 602
TPRGEIC+RGSTLFSGYHKRQDLT+DVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLS
Sbjct: 541 TPRGEICIRGSTLFSGYHKRQDLTDDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLS 600
Query: 603 QGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVVVPDQKALEDWARNHNVTGDFKS 662
QGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGV VPDQKALEDWA+NHNVTGDFKS
Sbjct: 601 QGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVAVPDQKALEDWAQNHNVTGDFKS 646
Query: 663 LCENLEARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLK 722
LCENL+ARKYILDELNSNGQKH LRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLK
Sbjct: 661 LCENLKARKYILDELNSNGQKHCLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLK 646
Query: 723 YYK 726
YYK
Sbjct: 721 YYK 646
BLAST of Lsi10G003310 vs. ExPASy TrEMBL
Match:
A0A5D3CQ95 (Long chain acyl-CoA synthetase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G001240 PE=4 SV=1)
HSP 1 Score: 1191.8 bits (3082), Expect = 0.0e+00
Identity = 605/726 (83.33%), Postives = 622/726 (85.67%), Query Frame = 0
Query: 3 MPTTYTVAVEAATPESGGRPSAGPIYRSIYAKDGLMQLPDGLESPWQFFRFVFFGHFFLL 62
M T YTV VEA TPE+GGRPSAGP+YRSIYAKDGL+QLPD LESPWQFF
Sbjct: 1 MSTPYTVVVEAETPETGGRPSAGPVYRSIYAKDGLLQLPDELESPWQFF----------- 60
Query: 63 KKGTIASHVYVIRLLLFVSNLISIATTVVPCGSFDLKSSNLSLIGGGELNSKSRRVNDSV 122
+DSV
Sbjct: 61 --------------------------------------------------------SDSV 120
Query: 123 KRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNC 182
KRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNC
Sbjct: 121 KRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNC 180
Query: 183 PEWIISMEVCN---FVFLRMYMRPVIAKLSLMYLCANAVEFIINHAEVSIAFVQENKLAS 242
PEWIISME CN ++ +Y L ANAVEFI+NHAEVSIAFVQENKLAS
Sbjct: 181 PEWIISMEACNSQAITYVPLYDT----------LGANAVEFIVNHAEVSIAFVQENKLAS 240
Query: 243 IFSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSFFSWEEFYQLVQYGSLDCELPTKQK 302
IFSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVS FSWEEFYQL GSLDCELP+KQK
Sbjct: 241 IFSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSCFSWEEFYQL---GSLDCELPSKQK 300
Query: 303 TNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPLSHVY 362
TNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKV TEEDSYFSFLPL+HVY
Sbjct: 301 TNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVATEEDSYFSFLPLAHVY 360
Query: 363 DQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGS 422
DQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGS
Sbjct: 361 DQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGS 420
Query: 423 LRKMLFDYAYNYKLGSLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPKHV 482
LRKMLFDYAY YKLG LKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLP+ V
Sbjct: 421 LRKMLFDYAYTYKLGYLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRLV 480
Query: 483 EEFLRVTSCATLSQGYGLTESCGGCFTSIANVFSMMGTVGVPVTTIEARLESVPDMGYDA 542
EEFLRVTSCATLSQGYGLTESCGGCFTSIANVF+M+GTVGVPVTTIEARLESVPDMGYDA
Sbjct: 481 EEFLRVTSCATLSQGYGLTESCGGCFTSIANVFTMVGTVGVPVTTIEARLESVPDMGYDA 540
Query: 543 LTGTPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIF 602
L TPRGEIC+RGSTLFSGYHKRQDLT+DVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIF
Sbjct: 541 LATTPRGEICIRGSTLFSGYHKRQDLTDDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIF 600
Query: 603 KLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVVVPDQKALEDWARNHNVTGD 662
KLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGV VPDQKALEDWA+NHNVTGD
Sbjct: 601 KLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVAVPDQKALEDWAQNHNVTGD 646
Query: 663 FKSLCENLEARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQ 722
FKSLCENL+ARKYILDELNSNGQKH LRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQ
Sbjct: 661 FKSLCENLKARKYILDELNSNGQKHRLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQ 646
Query: 723 LLKYYK 726
LLKYYK
Sbjct: 721 LLKYYK 646
BLAST of Lsi10G003310 vs. ExPASy TrEMBL
Match:
A0A1S3CAY4 (long chain acyl-CoA synthetase 2 OS=Cucumis melo OX=3656 GN=LOC103498426 PE=4 SV=1)
HSP 1 Score: 1191.8 bits (3082), Expect = 0.0e+00
Identity = 605/726 (83.33%), Postives = 622/726 (85.67%), Query Frame = 0
Query: 3 MPTTYTVAVEAATPESGGRPSAGPIYRSIYAKDGLMQLPDGLESPWQFFRFVFFGHFFLL 62
M T YTV VEA TPE+GGRPSAGP+YRSIYAKDGL+QLPD LESPWQFF
Sbjct: 1 MSTPYTVVVEAETPETGGRPSAGPVYRSIYAKDGLLQLPDELESPWQFF----------- 60
Query: 63 KKGTIASHVYVIRLLLFVSNLISIATTVVPCGSFDLKSSNLSLIGGGELNSKSRRVNDSV 122
+DSV
Sbjct: 61 --------------------------------------------------------SDSV 120
Query: 123 KRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNC 182
KRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNC
Sbjct: 121 KRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNC 180
Query: 183 PEWIISMEVCN---FVFLRMYMRPVIAKLSLMYLCANAVEFIINHAEVSIAFVQENKLAS 242
PEWIISME CN ++ +Y L ANAVEFI+NHAEVSIAFVQENKLAS
Sbjct: 181 PEWIISMEACNSQAITYVPLYDT----------LGANAVEFIVNHAEVSIAFVQENKLAS 240
Query: 243 IFSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSFFSWEEFYQLVQYGSLDCELPTKQK 302
IFSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVS FSWEEFYQL GSLDCELP+KQK
Sbjct: 241 IFSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSCFSWEEFYQL---GSLDCELPSKQK 300
Query: 303 TNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPLSHVY 362
TNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKV TEEDSYFSFLPL+HVY
Sbjct: 301 TNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVATEEDSYFSFLPLAHVY 360
Query: 363 DQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGS 422
DQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGS
Sbjct: 361 DQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGS 420
Query: 423 LRKMLFDYAYNYKLGSLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPKHV 482
LRKMLFDYAY YKLG LKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLP+ V
Sbjct: 421 LRKMLFDYAYTYKLGYLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRLV 480
Query: 483 EEFLRVTSCATLSQGYGLTESCGGCFTSIANVFSMMGTVGVPVTTIEARLESVPDMGYDA 542
EEFLRVTSCATLSQGYGLTESCGGCFTSIANVF+M+GTVGVPVTTIEARLESVPDMGYDA
Sbjct: 481 EEFLRVTSCATLSQGYGLTESCGGCFTSIANVFTMVGTVGVPVTTIEARLESVPDMGYDA 540
Query: 543 LTGTPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIF 602
L TPRGEIC+RGSTLFSGYHKRQDLT+DVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIF
Sbjct: 541 LATTPRGEICIRGSTLFSGYHKRQDLTDDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIF 600
Query: 603 KLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVVVPDQKALEDWARNHNVTGD 662
KLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGV VPDQKALEDWA+NHNVTGD
Sbjct: 601 KLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVAVPDQKALEDWAQNHNVTGD 646
Query: 663 FKSLCENLEARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQ 722
FKSLCENL+ARKYILDELNSNGQKH LRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQ
Sbjct: 661 FKSLCENLKARKYILDELNSNGQKHRLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQ 646
Query: 723 LLKYYK 726
LLKYYK
Sbjct: 721 LLKYYK 646
BLAST of Lsi10G003310 vs. ExPASy TrEMBL
Match:
A0A6J1JE62 (long chain acyl-CoA synthetase 2 OS=Cucurbita maxima OX=3661 GN=LOC111484205 PE=4 SV=1)
HSP 1 Score: 1185.6 bits (3066), Expect = 0.0e+00
Identity = 601/728 (82.55%), Postives = 619/728 (85.03%), Query Frame = 0
Query: 1 MTMPTTYTVAVEAATPESGGRPSAGPIYRSIYAKDGLMQLPDGLESPWQFFRFVFFGHFF 60
MTMPT YTVAVE ATPESGG+PSAGP+YRSIYAKDGL+QLP G ESPWQFF
Sbjct: 1 MTMPTAYTVAVEDATPESGGQPSAGPVYRSIYAKDGLLQLPTGFESPWQFF--------- 60
Query: 61 LLKKGTIASHVYVIRLLLFVSNLISIATTVVPCGSFDLKSSNLSLIGGGELNSKSRRVND 120
+D
Sbjct: 61 ----------------------------------------------------------SD 120
Query: 121 SVKRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGS 180
SVKRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYD+AIRMGSAIRK GVN GD CGIYGS
Sbjct: 121 SVKRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDSAIRMGSAIRKCGVNPGDCCGIYGS 180
Query: 181 NCPEWIISMEVCN---FVFLRMYMRPVIAKLSLMYLCANAVEFIINHAEVSIAFVQENKL 240
NCPEWIISME CN ++ +Y L ANAVEFIINHAEVSIAFVQENKL
Sbjct: 181 NCPEWIISMEACNSQAITYVPLYDT----------LGANAVEFIINHAEVSIAFVQENKL 240
Query: 241 ASIFSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSFFSWEEFYQLVQYGSLDCELPTK 300
ASIFSCLPRCS QLKTIVSF NVSSVHKKEAEELGVS +SWEEFYQL GSLDCELP+K
Sbjct: 241 ASIFSCLPRCSTQLKTIVSFNNVSSVHKKEAEELGVSCYSWEEFYQL---GSLDCELPSK 300
Query: 301 QKTNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPLSH 360
QKTNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDH+LLLTDKV TEEDSYFSFLPL+H
Sbjct: 301 QKTNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAH 360
Query: 361 VYDQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSG 420
VYDQIMETYCIYKGSSIGFWRGDIRFLL+DIQELKPTIFSGVPRVYDRIYSGILNKVSSG
Sbjct: 361 VYDQIMETYCIYKGSSIGFWRGDIRFLLNDIQELKPTIFSGVPRVYDRIYSGILNKVSSG 420
Query: 421 GSLRKMLFDYAYNYKLGSLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPK 480
GSLRKMLFDYAYNYKLG L KG+ QEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLP+
Sbjct: 421 GSLRKMLFDYAYNYKLGYLSKGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPR 480
Query: 481 HVEEFLRVTSCATLSQGYGLTESCGGCFTSIANVFSMMGTVGVPVTTIEARLESVPDMGY 540
HVEEFLRVTSCATLSQGYGLTESCGGC TSIANVF+MMGTVGVPVTTIE RLESVPDMGY
Sbjct: 481 HVEEFLRVTSCATLSQGYGLTESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGY 540
Query: 541 DALTGTPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKN 600
DAL+ TPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKN
Sbjct: 541 DALSSTPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKN 600
Query: 601 IFKLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVVVPDQKALEDWARNHNVT 660
IFKLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGV VPDQKALEDWARNHNVT
Sbjct: 601 IFKLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVT 648
Query: 661 GDFKSLCENLEARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKR 720
GDFKSLCENL ARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKR
Sbjct: 661 GDFKSLCENLNARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKR 648
Query: 721 PQLLKYYK 726
P+LLKYYK
Sbjct: 721 PRLLKYYK 648
BLAST of Lsi10G003310 vs. ExPASy TrEMBL
Match:
A0A6J1FW80 (long chain acyl-CoA synthetase 2 OS=Cucurbita moschata OX=3662 GN=LOC111449056 PE=4 SV=1)
HSP 1 Score: 1185.2 bits (3065), Expect = 0.0e+00
Identity = 602/728 (82.69%), Postives = 619/728 (85.03%), Query Frame = 0
Query: 1 MTMPTTYTVAVEAATPESGGRPSAGPIYRSIYAKDGLMQLPDGLESPWQFFRFVFFGHFF 60
MTMPT YTVAVE ATPESGG+PSAGP+YRSIYAKDGL+QL G ESPWQFF
Sbjct: 1 MTMPTAYTVAVEDATPESGGKPSAGPVYRSIYAKDGLLQLLAGFESPWQFF--------- 60
Query: 61 LLKKGTIASHVYVIRLLLFVSNLISIATTVVPCGSFDLKSSNLSLIGGGELNSKSRRVND 120
+D
Sbjct: 61 ----------------------------------------------------------SD 120
Query: 121 SVKRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGS 180
SVKRNPGNRMLGRR+TNGDSKAGSYVWLTYQEAYDAAIRMGSAIRK GVN GD CGIYGS
Sbjct: 121 SVKRNPGNRMLGRRRTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGS 180
Query: 181 NCPEWIISMEVCN---FVFLRMYMRPVIAKLSLMYLCANAVEFIINHAEVSIAFVQENKL 240
NCPEWIISME CN ++ +Y L ANAVEFIINHAEVSIAFVQENKL
Sbjct: 181 NCPEWIISMEACNSQAITYVPLYDT----------LGANAVEFIINHAEVSIAFVQENKL 240
Query: 241 ASIFSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSFFSWEEFYQLVQYGSLDCELPTK 300
ASIFSCLPRCS +LKTIVSFTNVSSVHKKEAEELGVS +SWEEFYQL GSLDCELP+K
Sbjct: 241 ASIFSCLPRCSTRLKTIVSFTNVSSVHKKEAEELGVSCYSWEEFYQL---GSLDCELPSK 300
Query: 301 QKTNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPLSH 360
QKTNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDH+LLLTDKV TEEDSYFSFLPL+H
Sbjct: 301 QKTNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAH 360
Query: 361 VYDQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSG 420
VYDQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSG
Sbjct: 361 VYDQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSG 420
Query: 421 GSLRKMLFDYAYNYKLGSLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPK 480
GSLRKMLFDYAYNYKLG L KG+ QEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLP+
Sbjct: 421 GSLRKMLFDYAYNYKLGYLSKGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPR 480
Query: 481 HVEEFLRVTSCATLSQGYGLTESCGGCFTSIANVFSMMGTVGVPVTTIEARLESVPDMGY 540
HVEEFLRVTSCATLSQGYGLTESCGGC TSIANVF+MMGTVGVPVTTIE RLESVPDMGY
Sbjct: 481 HVEEFLRVTSCATLSQGYGLTESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGY 540
Query: 541 DALTGTPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKN 600
DAL+ TPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKN
Sbjct: 541 DALSSTPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKN 600
Query: 601 IFKLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVVVPDQKALEDWARNHNVT 660
IFKLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGV VPDQKALEDWARNHNVT
Sbjct: 601 IFKLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVT 648
Query: 661 GDFKSLCENLEARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKR 720
GDFKSLCENL ARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKR
Sbjct: 661 GDFKSLCENLSARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKR 648
Query: 721 PQLLKYYK 726
PQLLKYYK
Sbjct: 721 PQLLKYYK 648
BLAST of Lsi10G003310 vs. NCBI nr
Match:
KAA0045965.1 (long chain acyl-CoA synthetase 2 [Cucumis melo var. makuwa])
HSP 1 Score: 1198.0 bits (3098), Expect = 0.0e+00
Identity = 607/723 (83.96%), Postives = 622/723 (86.03%), Query Frame = 0
Query: 3 MPTTYTVAVEAATPESGGRPSAGPIYRSIYAKDGLMQLPDGLESPWQFFRFVFFGHFFLL 62
M T YTV VEA TPE+GGRPSAGP+YRSIYAKDGL+QLPD LESPWQFF
Sbjct: 1 MSTPYTVVVEAETPETGGRPSAGPVYRSIYAKDGLLQLPDELESPWQFF----------- 60
Query: 63 KKGTIASHVYVIRLLLFVSNLISIATTVVPCGSFDLKSSNLSLIGGGELNSKSRRVNDSV 122
+DSV
Sbjct: 61 --------------------------------------------------------SDSV 120
Query: 123 KRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNC 182
KRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNC
Sbjct: 121 KRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNC 180
Query: 183 PEWIISMEVCNFVFLRMYMRPVIAKLSLMYLCANAVEFIINHAEVSIAFVQENKLASIFS 242
PEWIISMEVCNFV +A ANAVEFI+NHAEVSIAFVQENKLASIFS
Sbjct: 181 PEWIISMEVCNFVLSSCIDNQTVAG-------ANAVEFIVNHAEVSIAFVQENKLASIFS 240
Query: 243 CLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSFFSWEEFYQLVQYGSLDCELPTKQKTNI 302
CLPRCSNQLKTIVSFTNVSSVHKKEAEELGVS FSWEEFYQL GSLDCELP+KQKTNI
Sbjct: 241 CLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSCFSWEEFYQL---GSLDCELPSKQKTNI 300
Query: 303 CTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPLSHVYDQI 362
CTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKV TEEDSYFSFLPL+HVYDQI
Sbjct: 301 CTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVATEEDSYFSFLPLAHVYDQI 360
Query: 363 METYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRK 422
METYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRK
Sbjct: 361 METYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRK 420
Query: 423 MLFDYAYNYKLGSLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPKHVEEF 482
MLFDYAY YKLG LKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLP+ VEEF
Sbjct: 421 MLFDYAYTYKLGYLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRLVEEF 480
Query: 483 LRVTSCATLSQGYGLTESCGGCFTSIANVFSMMGTVGVPVTTIEARLESVPDMGYDALTG 542
LRVTSCATLSQGYGLTESCGGCFTSIANVF+M+GTVGVPVTTIEARLESVPDMGYDAL
Sbjct: 481 LRVTSCATLSQGYGLTESCGGCFTSIANVFTMVGTVGVPVTTIEARLESVPDMGYDALAT 540
Query: 543 TPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLS 602
TPRGEIC+RGSTLFSGYHKRQDLT+DVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLS
Sbjct: 541 TPRGEICIRGSTLFSGYHKRQDLTDDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLS 600
Query: 603 QGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVVVPDQKALEDWARNHNVTGDFKS 662
QGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGV VPDQKALEDWA+NHNVTGDFKS
Sbjct: 601 QGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVAVPDQKALEDWAQNHNVTGDFKS 646
Query: 663 LCENLEARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLK 722
LCENL+ARKYILDELNSNGQKH LRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLK
Sbjct: 661 LCENLKARKYILDELNSNGQKHCLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLK 646
Query: 723 YYK 726
YYK
Sbjct: 721 YYK 646
BLAST of Lsi10G003310 vs. NCBI nr
Match:
XP_038901459.1 (long chain acyl-CoA synthetase 2 [Benincasa hispida])
HSP 1 Score: 1195.6 bits (3092), Expect = 0.0e+00
Identity = 607/728 (83.38%), Postives = 626/728 (85.99%), Query Frame = 0
Query: 1 MTMPTTYTVAVEAATPESGGRPSAGPIYRSIYAKDGLMQLPDGLESPWQFFRFVFFGHFF 60
MTMPTTY VAVEAATP SGGRPSAGP+YRSIYAKDGL+QLPDGLESPWQFF
Sbjct: 1 MTMPTTYAVAVEAATPGSGGRPSAGPVYRSIYAKDGLLQLPDGLESPWQFF--------- 60
Query: 61 LLKKGTIASHVYVIRLLLFVSNLISIATTVVPCGSFDLKSSNLSLIGGGELNSKSRRVND 120
+D
Sbjct: 61 ----------------------------------------------------------SD 120
Query: 121 SVKRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGS 180
SVKRNPGNRMLGRRQTN DSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGS
Sbjct: 121 SVKRNPGNRMLGRRQTNDDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGS 180
Query: 181 NCPEWIISMEVCN---FVFLRMYMRPVIAKLSLMYLCANAVEFIINHAEVSIAFVQENKL 240
NCPEWIISME CN ++ +Y L ANAVEFIINHAEVSIAFVQE+KL
Sbjct: 181 NCPEWIISMEACNSQAITYIPLYDT----------LGANAVEFIINHAEVSIAFVQESKL 240
Query: 241 ASIFSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSFFSWEEFYQLVQYGSLDCELPTK 300
ASIFSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVS FSWEEF+QL GSLDC+LP+K
Sbjct: 241 ASIFSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSCFSWEEFHQL---GSLDCKLPSK 300
Query: 301 QKTNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPLSH 360
QKTNICTIMYTSGTTGEPKGV+LNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPL+H
Sbjct: 301 QKTNICTIMYTSGTTGEPKGVVLNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPLAH 360
Query: 361 VYDQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSG 420
VYDQIMETYCIYKGS IGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSG
Sbjct: 361 VYDQIMETYCIYKGSCIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSG 420
Query: 421 GSLRKMLFDYAYNYKLGSLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPK 480
GSLRKMLFDYAYNYKLG LKKGLL +KAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLP
Sbjct: 421 GSLRKMLFDYAYNYKLGYLKKGLLHDKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPS 480
Query: 481 HVEEFLRVTSCATLSQGYGLTESCGGCFTSIANVFSMMGTVGVPVTTIEARLESVPDMGY 540
HVEEFLRVTSCATLSQGYGLTESCGGCFTSIANVF+MMGTVGVPVTTIEARLESVPDMGY
Sbjct: 481 HVEEFLRVTSCATLSQGYGLTESCGGCFTSIANVFTMMGTVGVPVTTIEARLESVPDMGY 540
Query: 541 DALTGTPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKN 600
DAL+ TPRGEICLRGSTLFSGYHKR+DLTE+VLIDGWFHTGDIGEWQPDGSMKIIDRKKN
Sbjct: 541 DALSSTPRGEICLRGSTLFSGYHKRKDLTENVLIDGWFHTGDIGEWQPDGSMKIIDRKKN 600
Query: 601 IFKLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVVVPDQKALEDWARNHNVT 660
IFKLSQGEYVAVENIENIF QC LITSIWVYGNSFESFLVGVVVPDQKALEDWA+NHNVT
Sbjct: 601 IFKLSQGEYVAVENIENIFSQCSLITSIWVYGNSFESFLVGVVVPDQKALEDWAQNHNVT 648
Query: 661 GDFKSLCENLEARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKR 720
GDFKSLCENLEARKYILDELNSNGQKH LRGFELLKAI+LEPYPFDMERDLITPTFKLKR
Sbjct: 661 GDFKSLCENLEARKYILDELNSNGQKHRLRGFELLKAIYLEPYPFDMERDLITPTFKLKR 648
Query: 721 PQLLKYYK 726
PQLLKYYK
Sbjct: 721 PQLLKYYK 648
BLAST of Lsi10G003310 vs. NCBI nr
Match:
TYK13620.1 (long chain acyl-CoA synthetase 2 [Cucumis melo var. makuwa])
HSP 1 Score: 1191.8 bits (3082), Expect = 0.0e+00
Identity = 605/726 (83.33%), Postives = 622/726 (85.67%), Query Frame = 0
Query: 3 MPTTYTVAVEAATPESGGRPSAGPIYRSIYAKDGLMQLPDGLESPWQFFRFVFFGHFFLL 62
M T YTV VEA TPE+GGRPSAGP+YRSIYAKDGL+QLPD LESPWQFF
Sbjct: 1 MSTPYTVVVEAETPETGGRPSAGPVYRSIYAKDGLLQLPDELESPWQFF----------- 60
Query: 63 KKGTIASHVYVIRLLLFVSNLISIATTVVPCGSFDLKSSNLSLIGGGELNSKSRRVNDSV 122
+DSV
Sbjct: 61 --------------------------------------------------------SDSV 120
Query: 123 KRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNC 182
KRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNC
Sbjct: 121 KRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNC 180
Query: 183 PEWIISMEVCN---FVFLRMYMRPVIAKLSLMYLCANAVEFIINHAEVSIAFVQENKLAS 242
PEWIISME CN ++ +Y L ANAVEFI+NHAEVSIAFVQENKLAS
Sbjct: 181 PEWIISMEACNSQAITYVPLYDT----------LGANAVEFIVNHAEVSIAFVQENKLAS 240
Query: 243 IFSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSFFSWEEFYQLVQYGSLDCELPTKQK 302
IFSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVS FSWEEFYQL GSLDCELP+KQK
Sbjct: 241 IFSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSCFSWEEFYQL---GSLDCELPSKQK 300
Query: 303 TNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPLSHVY 362
TNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKV TEEDSYFSFLPL+HVY
Sbjct: 301 TNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVATEEDSYFSFLPLAHVY 360
Query: 363 DQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGS 422
DQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGS
Sbjct: 361 DQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGS 420
Query: 423 LRKMLFDYAYNYKLGSLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPKHV 482
LRKMLFDYAY YKLG LKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLP+ V
Sbjct: 421 LRKMLFDYAYTYKLGYLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRLV 480
Query: 483 EEFLRVTSCATLSQGYGLTESCGGCFTSIANVFSMMGTVGVPVTTIEARLESVPDMGYDA 542
EEFLRVTSCATLSQGYGLTESCGGCFTSIANVF+M+GTVGVPVTTIEARLESVPDMGYDA
Sbjct: 481 EEFLRVTSCATLSQGYGLTESCGGCFTSIANVFTMVGTVGVPVTTIEARLESVPDMGYDA 540
Query: 543 LTGTPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIF 602
L TPRGEIC+RGSTLFSGYHKRQDLT+DVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIF
Sbjct: 541 LATTPRGEICIRGSTLFSGYHKRQDLTDDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIF 600
Query: 603 KLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVVVPDQKALEDWARNHNVTGD 662
KLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGV VPDQKALEDWA+NHNVTGD
Sbjct: 601 KLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVAVPDQKALEDWAQNHNVTGD 646
Query: 663 FKSLCENLEARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQ 722
FKSLCENL+ARKYILDELNSNGQKH LRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQ
Sbjct: 661 FKSLCENLKARKYILDELNSNGQKHRLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQ 646
Query: 723 LLKYYK 726
LLKYYK
Sbjct: 721 LLKYYK 646
BLAST of Lsi10G003310 vs. NCBI nr
Match:
XP_008459243.1 (PREDICTED: long chain acyl-CoA synthetase 2 [Cucumis melo])
HSP 1 Score: 1191.8 bits (3082), Expect = 0.0e+00
Identity = 605/726 (83.33%), Postives = 622/726 (85.67%), Query Frame = 0
Query: 3 MPTTYTVAVEAATPESGGRPSAGPIYRSIYAKDGLMQLPDGLESPWQFFRFVFFGHFFLL 62
M T YTV VEA TPE+GGRPSAGP+YRSIYAKDGL+QLPD LESPWQFF
Sbjct: 1 MSTPYTVVVEAETPETGGRPSAGPVYRSIYAKDGLLQLPDELESPWQFF----------- 60
Query: 63 KKGTIASHVYVIRLLLFVSNLISIATTVVPCGSFDLKSSNLSLIGGGELNSKSRRVNDSV 122
+DSV
Sbjct: 61 --------------------------------------------------------SDSV 120
Query: 123 KRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNC 182
KRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNC
Sbjct: 121 KRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNC 180
Query: 183 PEWIISMEVCN---FVFLRMYMRPVIAKLSLMYLCANAVEFIINHAEVSIAFVQENKLAS 242
PEWIISME CN ++ +Y L ANAVEFI+NHAEVSIAFVQENKLAS
Sbjct: 181 PEWIISMEACNSQAITYVPLYDT----------LGANAVEFIVNHAEVSIAFVQENKLAS 240
Query: 243 IFSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSFFSWEEFYQLVQYGSLDCELPTKQK 302
IFSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVS FSWEEFYQL GSLDCELP+KQK
Sbjct: 241 IFSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSCFSWEEFYQL---GSLDCELPSKQK 300
Query: 303 TNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPLSHVY 362
TNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKV TEEDSYFSFLPL+HVY
Sbjct: 301 TNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVATEEDSYFSFLPLAHVY 360
Query: 363 DQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGS 422
DQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGS
Sbjct: 361 DQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGS 420
Query: 423 LRKMLFDYAYNYKLGSLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPKHV 482
LRKMLFDYAY YKLG LKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLP+ V
Sbjct: 421 LRKMLFDYAYTYKLGYLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPRLV 480
Query: 483 EEFLRVTSCATLSQGYGLTESCGGCFTSIANVFSMMGTVGVPVTTIEARLESVPDMGYDA 542
EEFLRVTSCATLSQGYGLTESCGGCFTSIANVF+M+GTVGVPVTTIEARLESVPDMGYDA
Sbjct: 481 EEFLRVTSCATLSQGYGLTESCGGCFTSIANVFTMVGTVGVPVTTIEARLESVPDMGYDA 540
Query: 543 LTGTPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIF 602
L TPRGEIC+RGSTLFSGYHKRQDLT+DVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIF
Sbjct: 541 LATTPRGEICIRGSTLFSGYHKRQDLTDDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIF 600
Query: 603 KLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVVVPDQKALEDWARNHNVTGD 662
KLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGV VPDQKALEDWA+NHNVTGD
Sbjct: 601 KLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVAVPDQKALEDWAQNHNVTGD 646
Query: 663 FKSLCENLEARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQ 722
FKSLCENL+ARKYILDELNSNGQKH LRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQ
Sbjct: 661 FKSLCENLKARKYILDELNSNGQKHRLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQ 646
Query: 723 LLKYYK 726
LLKYYK
Sbjct: 721 LLKYYK 646
BLAST of Lsi10G003310 vs. NCBI nr
Match:
XP_023512617.1 (long chain acyl-CoA synthetase 2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1186.4 bits (3068), Expect = 0.0e+00
Identity = 602/728 (82.69%), Postives = 617/728 (84.75%), Query Frame = 0
Query: 1 MTMPTTYTVAVEAATPESGGRPSAGPIYRSIYAKDGLMQLPDGLESPWQFFRFVFFGHFF 60
MTMPT YTVAVE ATPESGG+PS GP+YRSIYAKDGL+QLP G ESPWQFF
Sbjct: 1 MTMPTAYTVAVEDATPESGGKPSGGPVYRSIYAKDGLLQLPTGFESPWQFF--------- 60
Query: 61 LLKKGTIASHVYVIRLLLFVSNLISIATTVVPCGSFDLKSSNLSLIGGGELNSKSRRVND 120
+D
Sbjct: 61 ----------------------------------------------------------SD 120
Query: 121 SVKRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGS 180
SVKRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRK GVN GD CGIYGS
Sbjct: 121 SVKRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKCGVNPGDCCGIYGS 180
Query: 181 NCPEWIISMEVCN---FVFLRMYMRPVIAKLSLMYLCANAVEFIINHAEVSIAFVQENKL 240
NCPEWIISME CN ++ +Y L ANAVEFIINHAEVSIAFVQENKL
Sbjct: 181 NCPEWIISMEACNSQAITYVPLYDT----------LGANAVEFIINHAEVSIAFVQENKL 240
Query: 241 ASIFSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSFFSWEEFYQLVQYGSLDCELPTK 300
ASIFSCLPRCS QLKTIVSF NVSSVHKKEAEELGVS +SWEEFYQL GSLDCELP+K
Sbjct: 241 ASIFSCLPRCSTQLKTIVSFNNVSSVHKKEAEELGVSCYSWEEFYQL---GSLDCELPSK 300
Query: 301 QKTNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPLSH 360
KTNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDH+LLLTDKV TEEDSYFSFLPL+H
Sbjct: 301 LKTNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHILLLTDKVATEEDSYFSFLPLAH 360
Query: 361 VYDQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSG 420
VYDQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSG
Sbjct: 361 VYDQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSG 420
Query: 421 GSLRKMLFDYAYNYKLGSLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPK 480
GSLRKMLFDYAYNYKLG L KG+ QEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLP+
Sbjct: 421 GSLRKMLFDYAYNYKLGYLSKGVPQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPR 480
Query: 481 HVEEFLRVTSCATLSQGYGLTESCGGCFTSIANVFSMMGTVGVPVTTIEARLESVPDMGY 540
HVEEFLRVTSCATLSQGYGLTESCGGC TSIANVF+MMGTVGVPVTTIE RLESVPDMGY
Sbjct: 481 HVEEFLRVTSCATLSQGYGLTESCGGCLTSIANVFTMMGTVGVPVTTIEVRLESVPDMGY 540
Query: 541 DALTGTPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKN 600
DAL+ TPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKN
Sbjct: 541 DALSSTPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKN 600
Query: 601 IFKLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVVVPDQKALEDWARNHNVT 660
IFKLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGV VPDQKALEDWARNHNVT
Sbjct: 601 IFKLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVAVPDQKALEDWARNHNVT 648
Query: 661 GDFKSLCENLEARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKR 720
GDFKSLCENL ARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKR
Sbjct: 661 GDFKSLCENLNARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKR 648
Query: 721 PQLLKYYK 726
PQLLKYYK
Sbjct: 721 PQLLKYYK 648
BLAST of Lsi10G003310 vs. TAIR 10
Match:
AT1G49430.1 (long-chain acyl-CoA synthetase 2 )
HSP 1 Score: 858.6 bits (2217), Expect = 4.1e-249
Identity = 432/729 (59.26%), Postives = 530/729 (72.70%), Query Frame = 0
Query: 1 MTMPTTYTVAVEAATPESGGRPSAGPIYRSIYAKDGLMQLPDGLESPWQFFRFVFFGHFF 60
M++ + VE P + PSAGP+YR YAKDGL+ LP ++SPWQFF
Sbjct: 1 MSLAADNVLLVEEGRPATAEHPSAGPVYRCKYAKDGLLDLPTDIDSPWQFF--------- 60
Query: 61 LLKKGTIASHVYVIRLLLFVSNLISIATTVVPCGSFDLKSSNLSLIGGGELNSKSRRVND 120
++
Sbjct: 61 ----------------------------------------------------------SE 120
Query: 121 SVKRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGS 180
+VK+ P +MLG+R T DSK G Y W+TY+EA+DAAIR+GSAIR RGV+ G CGIYG+
Sbjct: 121 AVKKYPNEQMLGQRVTT-DSKVGPYTWITYKEAHDAAIRIGSAIRSRGVDPGHCCGIYGA 180
Query: 181 NCPEWIISMEVCNFVFLRMYMRPVIAKLSLM-YLCANAVEFIINHAEVSIAFVQENKLAS 240
NCPEWII+ME C M I + L L NAVEFIINHAEVS+ FVQE ++S
Sbjct: 181 NCPEWIIAMEAC--------MSQGITYVPLYDSLGVNAVEFIINHAEVSLVFVQEKTVSS 240
Query: 241 IFSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSFFSWEEFYQLVQYGSLD-CELPTKQ 300
I SC CS+ LKTIVSF VSS K+EA+ VS FSW EF + G+LD LP K+
Sbjct: 241 ILSCQKGCSSNLKTIVSFGEVSSTQKEEAKNQCVSLFSWNEFSLM---GNLDEANLPRKR 300
Query: 301 KTNICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPLSHV 360
KT+ICTIMYTSGTTGEPKGVILNNAAI +VLS+D +L +TD+ D +FS+LPL+H
Sbjct: 301 KTDICTIMYTSGTTGEPKGVILNNAAISVQVLSIDKMLEVTDRSCDTSDVFFSYLPLAHC 360
Query: 361 YDQIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGG 420
YDQ+ME Y + +GSS+G+WRGDIR+L+DD+Q LKPT+F GVPRVYD++Y+GI+ K+S+ G
Sbjct: 361 YDQVMEIYFLSRGSSVGYWRGDIRYLMDDVQALKPTVFCGVPRVYDKLYAGIMQKISASG 420
Query: 421 SLRKMLFDYAYNYKLGSLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPKH 480
+RK LFD+AYNYKLG+++KG QE+A+P LD+L+FDKIKEA GGR + LSGAAPLP+H
Sbjct: 421 LIRKKLFDFAYNYKLGNMRKGFSQEEASPRLDRLMFDKIKEALGGRAHMLLSGAAPLPRH 480
Query: 481 VEEFLRVTSCATLSQGYGLTESCGGCFTSIANVFSMMGTVGVPVTTIEARLESVPDMGYD 540
VEEFLR+ + LSQGYGLTESCGG FT++A VFSM+GTVGVP+ T+EARL SVP+MGYD
Sbjct: 481 VEEFLRIIPASNLSQGYGLTESCGGSFTTLAGVFSMVGTVGVPMPTVEARLVSVPEMGYD 540
Query: 541 ALTG-TPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKN 600
A + PRGEICLRG+++FSGYHKRQDLT+ VLIDGWFHTGDIGEWQ DGSMKIIDRKKN
Sbjct: 541 AFSADVPRGEICLRGNSMFSGYHKRQDLTDQVLIDGWFHTGDIGEWQEDGSMKIIDRKKN 600
Query: 601 IFKLSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVVVPDQKALEDWAR-NHNV 660
IFKLSQGEYVAVEN+EN + +CPLI IWVYGNSFESFLVGVVVPD+KA+EDWA+ N+
Sbjct: 601 IFKLSQGEYVAVENLENTYSRCPLIAQIWVYGNSFESFLVGVVVPDRKAIEDWAKLNYQS 650
Query: 661 TGDFKSLCENLEARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLK 720
DF+SLC+NL+A+KY LDELNS +++ L+GFE+LKAIHLEP PFD+ERDLITPTFKLK
Sbjct: 661 PNDFESLCQNLKAQKYFLDELNSTAKQYQLKGFEMLKAIHLEPNPFDIERDLITPTFKLK 650
Query: 721 RPQLLKYYK 726
RPQLL++YK
Sbjct: 721 RPQLLQHYK 650
BLAST of Lsi10G003310 vs. TAIR 10
Match:
AT1G64400.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 731.9 bits (1888), Expect = 5.7e-211
Identity = 364/720 (50.56%), Postives = 479/720 (66.53%), Query Frame = 0
Query: 7 YTVAVEAATP-ESGGRPSAGPIYRSIYAKDGLMQLPDGLESPWQFFRFVFFGHFFLLKKG 66
Y V VE GG PS GP+YRSIYAKDG + PD L S W FR
Sbjct: 6 YIVEVEKGKQGVDGGSPSVGPVYRSIYAKDGFPEPPDDLVSAWDIFRL------------ 65
Query: 67 TIASHVYVIRLLLFVSNLISIATTVVPCGSFDLKSSNLSLIGGGELNSKSRRVNDSVKRN 126
SV+++
Sbjct: 66 -------------------------------------------------------SVEKS 125
Query: 127 PGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGSNCPEW 186
P N MLGRR+ D KAG YVW TY+E ++ I++G++IR GV GD+CGIYG+N PEW
Sbjct: 126 PNNPMLGRREI-VDGKAGKYVWQTYKEVHNVVIKLGNSIRTIGVGKGDKCGIYGANSPEW 185
Query: 187 IISMEVCNFVFLRMYMRPVIAKLSLMYLCANAVEFIINHAEVSIAFVQENKLASIFSCLP 246
IISME CN L Y P+ L A A+EFII HAEVS+AF +ENK++ + P
Sbjct: 186 IISMEACNAHGL--YCVPLYDTLG-----AGAIEFIICHAEVSLAFAEENKISELLKTAP 245
Query: 247 RCSNQLKTIVSFTNVSSVHKKEAEELGVSFFSWEEFYQLVQYGSLDCELPTKQKTNICTI 306
+ + LK IVSF V++ + EAE ++ +SW++F +L + ELP K+++++CTI
Sbjct: 246 KSTKYLKYIVSFGEVTNNQRVEAERHRLTIYSWDQFLKLGE--GKHYELPEKRRSDVCTI 305
Query: 307 MYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPLSHVYDQIMET 366
MYTSGTTG+PKGV+L N +I+ + V LL D+ T +D Y S+LPL+H++D+++E
Sbjct: 306 MYTSGTTGDPKGVLLTNESIIHLLEGVKKLLKTIDEELTSKDVYLSYLPLAHIFDRVIEE 365
Query: 367 YCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSLRKMLF 426
CIY+ +SIGFWRGD++ L++DI LKPT+F VPRV +RIY+G+ K+S GG ++K LF
Sbjct: 366 LCIYEAASIGFWRGDVKILIEDIAALKPTVFCAVPRVLERIYTGLQQKLSDGGFVKKKLF 425
Query: 427 DYAYNYKLGSLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPKHVEEFLRV 486
++A+ YK +++KG E+A+P+ DK+VF K+KE GG VRL LSGAAPL H+E FLRV
Sbjct: 426 NFAFKYKHKNMEKGQPHEQASPIADKIVFKKVKEGLGGNVRLILSGAAPLAAHIESFLRV 485
Query: 487 TSCATLSQGYGLTESCGGCFTSIANVFSMMGTVGVPVTTIEARLESVPDMGYDALTGTPR 546
+CA + QGYGLTESCGG F SI N SM+GTVG PV ++ RLESVP+MGYDAL PR
Sbjct: 486 VACAHVLQGYGLTESCGGTFVSIPNELSMLGTVGPPVPNVDIRLESVPEMGYDALASNPR 545
Query: 547 GEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFKLSQGE 606
GEIC+RG TLFSGY+KR+DLT++V IDGW HTGD+GEWQPDG+MKIIDRKKNIFKLSQGE
Sbjct: 546 GEICIRGKTLFSGYYKREDLTQEVFIDGWLHTGDVGEWQPDGAMKIIDRKKNIFKLSQGE 605
Query: 607 YVAVENIENIFLQCPLITSIWVYGNSFESFLVGVVVPDQKALEDWARNHNVTGDFKSLCE 666
YVAVEN+ENI+ I SIWVYGNS+ES+LV VV P + +E WA+ H V+GDF+S+C
Sbjct: 606 YVAVENLENIYSHVAAIESIWVYGNSYESYLVAVVCPSKIQIEHWAKEHKVSGDFESICR 648
Query: 667 NLEARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQLLKYYK 726
N + ++++L E N + L+GFEL+K +HL+ PFDMERDLITP++K+KRPQLLKYY+
Sbjct: 666 NQKTKEFVLGEFNRVAKDKKLKGFELIKGVHLDTVPFDMERDLITPSYKMKRPQLLKYYQ 648
BLAST of Lsi10G003310 vs. TAIR 10
Match:
AT4G23850.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 728.4 bits (1879), Expect = 6.3e-210
Identity = 364/728 (50.00%), Postives = 480/728 (65.93%), Query Frame = 0
Query: 1 MTMPTTYTVAVEAATPESGGRPSAGPIYRSIYAKDGLMQLPDGLESPWQFFRFVFFGHFF 60
M+ Y VE S GRPS GP+YRSI+AKDG +G++S W FR
Sbjct: 1 MSQQKKYIFQVEEGKEGSDGRPSVGPVYRSIFAKDGFPDPIEGMDSCWDVFRM------- 60
Query: 61 LLKKGTIASHVYVIRLLLFVSNLISIATTVVPCGSFDLKSSNLSLIGGGELNSKSRRVND 120
Sbjct: 61 ------------------------------------------------------------ 120
Query: 121 SVKRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGS 180
SV++ P N MLGRR+ D K G YVW TYQE YD +++G+++R GV +CGIYG+
Sbjct: 121 SVEKYPNNPMLGRREI-VDGKPGKYVWQTYQEVYDIVMKLGNSLRSVGVKDEAKCGIYGA 180
Query: 181 NCPEWIISMEVCNFVFLRMYMRPVIAKLSLMYLCANAVEFIINHAEVSIAFVQENKLASI 240
N PEWIISME CN L Y P+ L A+AVEFII+H+EVSI FV+E K++ +
Sbjct: 181 NSPEWIISMEACNAHGL--YCVPLYDTLG-----ADAVEFIISHSEVSIVFVEEKKISEL 240
Query: 241 FSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSFFSWEEFYQLVQYGSLDCELPTKQKT 300
F P + +KT+VSF VS K+EAE G+ ++W+EF +L + D LP K+K+
Sbjct: 241 FKTCPNSTEYMKTVVSFGGVSREQKEEAETFGLVIYAWDEFLKLGEGKQYD--LPIKKKS 300
Query: 301 NICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPLSHVYD 360
+ICTIMYTSGTTG+PKGV+++N +I+ + V LL ++ T +D Y S+LPL+H++D
Sbjct: 301 DICTIMYTSGTTGDPKGVMISNESIVTLIAGVIRLLKSANEALTVKDVYLSYLPLAHIFD 360
Query: 361 QIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSL 420
+++E I G++IGFWRGD++ L++D+ ELKPTIF VPRV DR+YSG+ K+S GG L
Sbjct: 361 RVIEECFIQHGAAIGFWRGDVKLLIEDLAELKPTIFCAVPRVLDRVYSGLQKKLSDGGFL 420
Query: 421 RKMLFDYAYNYKLGSLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPKHVE 480
+K +FD A++YK G +KKG +A+PL DKLVF K+K+ GG VR+ LSGAAPL HVE
Sbjct: 421 KKFIFDSAFSYKFGYMKKGQSHVEASPLFDKLVFSKVKQGLGGNVRIILSGAAPLASHVE 480
Query: 481 EFLRVTSCATLSQGYGLTESCGGCFTSIANVFSMMGTVGVPVTTIEARLESVPDMGYDAL 540
FLRV +C + QGYGLTESC G F S+ + M+GTVG PV ++ RLESVP+M YDAL
Sbjct: 481 SFLRVVACCHVLQGYGLTESCAGTFVSLPDELGMLGTVGPPVPNVDIRLESVPEMEYDAL 540
Query: 541 TGTPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFK 600
T RGEIC+RG TLFSGY+KR+DLT++VLIDGW HTGD+GEWQPDGSMKIIDRKKNIFK
Sbjct: 541 ASTARGEICIRGKTLFSGYYKREDLTKEVLIDGWLHTGDVGEWQPDGSMKIIDRKKNIFK 600
Query: 601 LSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVVVPDQKALEDWARNHNVTGDF 660
LSQGEYVAVENIENI+ + + S+WVYGNSFESFL+ + P+Q LE WA + V+GD+
Sbjct: 601 LSQGEYVAVENIENIYGEVQAVDSVWVYGNSFESFLIAIANPNQHILERWAAENGVSGDY 651
Query: 661 KSLCENLEARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQL 720
+LC+N +A+++IL EL ++ ++GFE++KAIHL+P PFDMERDL+TPTFK KRPQL
Sbjct: 661 DALCQNEKAKEFILGELVKMAKEKKMKGFEIIKAIHLDPVPFDMERDLLTPTFKKKRPQL 651
Query: 721 LKYYKAAL 729
LKYY++ +
Sbjct: 721 LKYYQSVI 651
BLAST of Lsi10G003310 vs. TAIR 10
Match:
AT4G11030.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 719.2 bits (1855), Expect = 3.8e-207
Identity = 359/725 (49.52%), Postives = 473/725 (65.24%), Query Frame = 0
Query: 1 MTMPTTYTVAVEAATPESGGRPSAGPIYRSIYAKDGLMQLPDGLESPWQFFRFVFFGHFF 60
MT + VEAA + G PS GP+YRS +A++G DG++S W FR
Sbjct: 1 MTSQKRFIFEVEAAKEATDGNPSVGPVYRSTFAQNGFPNPIDGIQSCWDIFR-------- 60
Query: 61 LLKKGTIASHVYVIRLLLFVSNLISIATTVVPCGSFDLKSSNLSLIGGGELNSKSRRVND 120
Sbjct: 61 -----------------------------------------------------------T 120
Query: 121 SVKRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGS 180
+V++ P NRMLGRR+ + + KAG YVW TY+E YD I++G+++R G+ G++CGIYG
Sbjct: 121 AVEKYPNNRMLGRREIS-NGKAGKYVWKTYKEVYDIVIKLGNSLRSCGIKEGEKCGIYGI 180
Query: 181 NCPEWIISMEVCNFVFLRMYMRPVIAKLSLMYLCANAVEFIINHAEVSIAFVQENKLASI 240
NC EWIISME CN L Y P+ L A AVEFII+HAEVSIAFV+E K+ +
Sbjct: 181 NCCEWIISMEACNAHGL--YCVPLYDTLG-----AGAVEFIISHAEVSIAFVEEKKIPEL 240
Query: 241 FSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSFFSWEEFYQLVQYGSLDCELPTKQKT 300
F P + +KT+VSF V K+EAE+LG+ SW+EF +L + ELP K+ +
Sbjct: 241 FKTCPNSTKYMKTVVSFGGVKPEQKEEAEKLGLVIHSWDEFLKLGE--GKQYELPIKKPS 300
Query: 301 NICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPLSHVYD 360
+ICTIMYTSGTTG+PKGV+++N +I+ V H L + +E+D Y S+LPL+HV+D
Sbjct: 301 DICTIMYTSGTTGDPKGVMISNESIVTITTGVMHFLGNVNASLSEKDVYISYLPLAHVFD 360
Query: 361 QIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSL 420
+ +E I G SIGFWRGD++ L++D+ ELKP+IF VPRV DR+Y+G+ K+S GG
Sbjct: 361 RAIEECIIQVGGSIGFWRGDVKLLIEDLGELKPSIFCAVPRVLDRVYTGLQQKLSGGGFF 420
Query: 421 RKMLFDYAYNYKLGSLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPKHVE 480
+K +FD A++YK G++KKG A+P DKLVF+K+K+ GG VR+ LSGAAPL H+E
Sbjct: 421 KKKVFDVAFSYKFGNMKKGQSHVAASPFCDKLVFNKVKQGLGGNVRIILSGAAPLASHIE 480
Query: 481 EFLRVTSCATLSQGYGLTESCGGCFTSIANVFSMMGTVGVPVTTIEARLESVPDMGYDAL 540
FLRV +C + QGYGLTESC G F + + M+GTVG PV ++ RLESVP+M YDAL
Sbjct: 481 SFLRVVACCNVLQGYGLTESCAGTFATFPDELDMLGTVGPPVPNVDIRLESVPEMNYDAL 540
Query: 541 TGTPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFK 600
TPRGEIC+RG TLFSGY+KR+DLT++V IDGW HTGD+GEWQP+GSMKIIDRKKNIFK
Sbjct: 541 GSTPRGEICIRGKTLFSGYYKREDLTKEVFIDGWLHTGDVGEWQPNGSMKIIDRKKNIFK 600
Query: 601 LSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVVVPDQKALEDWARNHNVTGDF 660
L+QGEYVAVEN+EN++ Q +I SIWVYGNSFESFLV + P Q+ LE WA + V GDF
Sbjct: 601 LAQGEYVAVENLENVYSQVEVIESIWVYGNSFESFLVAIANPAQQTLERWAVENGVNGDF 648
Query: 661 KSLCENLEARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQL 720
S+C+N +A+ +IL EL +++ L+GFE++K +HLEP FDMERDL+TPT+K KRPQL
Sbjct: 661 NSICQNAKAKAFILGELVKTAKENKLKGFEIIKDVHLEPVAFDMERDLLTPTYKKKRPQL 648
Query: 721 LKYYK 726
LKYY+
Sbjct: 721 LKYYQ 648
BLAST of Lsi10G003310 vs. TAIR 10
Match:
AT2G47240.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 567.0 bits (1460), Expect = 2.4e-161
Identity = 283/608 (46.55%), Postives = 394/608 (64.80%), Query Frame = 0
Query: 121 SVKRNPGNRMLGRRQTNGDSKAGSYVWLTYQEAYDAAIRMGSAIRKRGVNSGDRCGIYGS 180
SV++ P N MLG R+ D K G Y+W TY+E Y+ +++GSA+R G G R GIYG
Sbjct: 51 SVEKFPDNNMLGWRRI-VDEKVGPYMWKTYKEVYEEVLQIGSALRAAGAEPGSRVGIYGV 110
Query: 181 NCPEWIISMEVCNFVFLRMYMRPVIAKLSLMYLCANAVEFIINHAEVSIAFVQENKLASI 240
NCP+WII+ME C +I L + AV++I+ HAE+ FVQ+ K+ +
Sbjct: 111 NCPQWIIAMEAC-------AAHTLICVPLYDTLGSGAVDYIVEHAEIDFVFVQDTKIKGL 170
Query: 241 FSCLPRCSNQLKTIVSFTNVSSVHKKEAEELGVSFFSWEEFYQLVQYGSLDCELPTKQKT 300
+C+ +LK IVSFTNVS +A E+GV +SW +F + + D P +
Sbjct: 171 LEPDCKCAKRLKAIVSFTNVSDELSHKASEIGVKTYSWIDFLHMGREKPEDTNPP--KAF 230
Query: 301 NICTIMYTSGTTGEPKGVILNNAAIMAEVLSVDHLLLLTDKVGTEEDSYFSFLPLSHVYD 360
NICTIMYTSGT+G+PKGV+L + A+ V+ +D + + T +D Y SFLPL+H+ D
Sbjct: 231 NICTIMYTSGTSGDPKGVVLTHQAVATFVVGMDLYMDQFEDKMTHDDVYLSFLPLAHILD 290
Query: 361 QIMETYCIYKGSSIGFWRGDIRFLLDDIQELKPTIFSGVPRVYDRIYSGILNKVSSGGSL 420
++ E Y KG+S+G++ G++ L DDIQELKPT +GVPRV++RI+ GI +
Sbjct: 291 RMNEEYFFRKGASVGYYHGNLNVLRDDIQELKPTYLAGVPRVFERIHEGIQKALQELNPR 350
Query: 421 RKMLFDYAYNYKLGSLKKGLLQEKAAPLLDKLVFDKIKEAFGGRVRLFLSGAAPLPKHVE 480
R+ +F+ Y +KL L +G KA+P+ D + F KI++ GGR+RL +SG APL +E
Sbjct: 351 RRFIFNALYKHKLAWLNRGYSHSKASPMADFIAFRKIRDKLGGRIRLLVSGGAPLSPEIE 410
Query: 481 EFLRVTSCATLSQGYGLTESCGGCFTSIANVFSMMGTVGVPVTTIEARLESVPDMGYDAL 540
EFLRVT C + QGYGLTE+ GG + M+GTVG+P E RLE V +MGYD L
Sbjct: 411 EFLRVTCCCFVVQGYGLTETLGGTALGFPDEMCMLGTVGIPAVYNEIRLEEVSEMGYDPL 470
Query: 541 TGTPRGEICLRGSTLFSGYHKRQDLTEDVLIDGWFHTGDIGEWQPDGSMKIIDRKKNIFK 600
P GEIC+RG +FSGY+K +LTE+V+ DGWFHTGDIGE P+G +KIIDRKKN+ K
Sbjct: 471 GENPAGEICIRGQCMFSGYYKNPELTEEVMKDGWFHTGDIGEILPNGVLKIIDRKKNLIK 530
Query: 601 LSQGEYVAVENIENIFLQCPLITSIWVYGNSFESFLVGVVVPDQKALEDWARNHNVTGDF 660
LSQGEYVA+E++ENIF Q ++ IWVYG+SF+S LV VVVP+ + + WA++ T F
Sbjct: 531 LSQGEYVALEHLENIFGQNSVVQDIWVYGDSFKSMLVAVVVPNPETVNRWAKDLGFTKPF 590
Query: 661 KSLCENLEARKYILDELNSNGQKHHLRGFELLKAIHLEPYPFDMERDLITPTFKLKRPQL 720
+ LC E +++I+ EL S +K+ LR FE +KA+ +E PFD+ERDL+T T K +R L
Sbjct: 591 EELCSFPELKEHIISELKSTAEKNKLRKFEYIKAVTVETKPFDVERDLVTATLKNRRNNL 648
Query: 721 LKYYKAAL 729
LKYY+ +
Sbjct: 651 LKYYQVQI 648
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| M4ISH1 | 2.5e-280 | 66.67 | Probable CoA ligase CCL6 OS=Humulus lupulus OX=3486 GN=CCL6 PE=2 SV=1 | [more] |
| Q9XIA9 | 5.7e-248 | 59.26 | Long chain acyl-CoA synthetase 2 OS=Arabidopsis thaliana OX=3702 GN=LACS2 PE=2 S... | [more] |
| Q9C7W4 | 8.1e-210 | 50.56 | Long chain acyl-CoA synthetase 3 OS=Arabidopsis thaliana OX=3702 GN=LACS3 PE=2 S... | [more] |
| Q9T0A0 | 8.9e-209 | 50.00 | Long chain acyl-CoA synthetase 4 OS=Arabidopsis thaliana OX=3702 GN=LACS4 PE=2 S... | [more] |
| Q9T009 | 5.4e-206 | 49.52 | Long chain acyl-CoA synthetase 5 OS=Arabidopsis thaliana OX=3702 GN=LACS5 PE=2 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A5A7TX35 | 0.0e+00 | 83.96 | Long chain acyl-CoA synthetase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... | [more] |
| A0A5D3CQ95 | 0.0e+00 | 83.33 | Long chain acyl-CoA synthetase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... | [more] |
| A0A1S3CAY4 | 0.0e+00 | 83.33 | long chain acyl-CoA synthetase 2 OS=Cucumis melo OX=3656 GN=LOC103498426 PE=4 SV... | [more] |
| A0A6J1JE62 | 0.0e+00 | 82.55 | long chain acyl-CoA synthetase 2 OS=Cucurbita maxima OX=3661 GN=LOC111484205 PE=... | [more] |
| A0A6J1FW80 | 0.0e+00 | 82.69 | long chain acyl-CoA synthetase 2 OS=Cucurbita moschata OX=3662 GN=LOC111449056 P... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| KAA0045965.1 | 0.0e+00 | 83.96 | long chain acyl-CoA synthetase 2 [Cucumis melo var. makuwa] | [more] |
| XP_038901459.1 | 0.0e+00 | 83.38 | long chain acyl-CoA synthetase 2 [Benincasa hispida] | [more] |
| TYK13620.1 | 0.0e+00 | 83.33 | long chain acyl-CoA synthetase 2 [Cucumis melo var. makuwa] | [more] |
| XP_008459243.1 | 0.0e+00 | 83.33 | PREDICTED: long chain acyl-CoA synthetase 2 [Cucumis melo] | [more] |
| XP_023512617.1 | 0.0e+00 | 82.69 | long chain acyl-CoA synthetase 2 [Cucurbita pepo subsp. pepo] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT1G49430.1 | 4.1e-249 | 59.26 | long-chain acyl-CoA synthetase 2 | [more] |
| AT1G64400.1 | 5.7e-211 | 50.56 | AMP-dependent synthetase and ligase family protein | [more] |
| AT4G23850.1 | 6.3e-210 | 50.00 | AMP-dependent synthetase and ligase family protein | [more] |
| AT4G11030.1 | 3.8e-207 | 49.52 | AMP-dependent synthetase and ligase family protein | [more] |
| AT2G47240.1 | 2.4e-161 | 46.55 | AMP-dependent synthetase and ligase family protein | [more] |
