Lsi10G003000 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G003000
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionAbhydrolase_3 domain-containing protein
Locationchr10: 4821952 .. 4822215 (-)
RNA-Seq ExpressionLsi10G003000
SyntenyLsi10G003000
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTAATAGACGATAGAGCCTTCTCTGTCGCCTCTGCCGCGTCTCCAAATTATCACCGCCACTTGGTCTCCTTCGCCGCCGAGGCCAACATTGTCGCTATCTCCGTTGAATACCAACTTGCGCGGGAGAATTCTCTCTCGACGACATACGACGACGCGTGGGCGGTGGTGGAAGGGGCAGCCTCGCACTGCAATCGCGACGACGCTAAGATTTGGCTGAACGAACACACGAACTTTGACCATGTATTCATCGTTCAGGGATAA

mRNA sequence

ATGCTAATAGACGATAGAGCCTTCTCTGTCGCCTCTGCCGCGTCTCCAAATTATCACCGCCACTTGGTCTCCTTCGCCGCCGAGGCCAACATTGTCGCTATCTCCGTTGAATACCAACTTGCGCGGGAGAATTCTCTCTCGACGACATACGACGACGCGTGGGCGGTGGTGGAAGGGGCAGCCTCGCACTGCAATCGCGACGACGCTAAGATTTGGCTGAACGAACACACGAACTTTGACCATGTATTCATCGTTCAGGGATAA

Coding sequence (CDS)

ATGCTAATAGACGATAGAGCCTTCTCTGTCGCCTCTGCCGCGTCTCCAAATTATCACCGCCACTTGGTCTCCTTCGCCGCCGAGGCCAACATTGTCGCTATCTCCGTTGAATACCAACTTGCGCGGGAGAATTCTCTCTCGACGACATACGACGACGCGTGGGCGGTGGTGGAAGGGGCAGCCTCGCACTGCAATCGCGACGACGCTAAGATTTGGCTGAACGAACACACGAACTTTGACCATGTATTCATCGTTCAGGGATAA

Protein sequence

MLIDDRAFSVASAASPNYHRHLVSFAAEANIVAISVEYQLARENSLSTTYDDAWAVVEGAASHCNRDDAKIWLNEHTNFDHVFIVQG
Homology
BLAST of Lsi10G003000 vs. ExPASy Swiss-Prot
Match: A0A0H5BMX5 (Tuliposide B-converting enzyme 1, amyloplastic OS=Tulipa gesneriana OX=13306 GN=TCEB1 PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 9.1e-11
Identity = 32/78 (41.03%), Postives = 47/78 (60.26%), Query Frame = 0

Query: 7   AFSVASAASPNYHRHLVSFAAEANIVAISVEYQLARENSLSTTYDDAWAVVEGAASHCNR 66
           AF V  +ASP YH +L + A++ANI+ +SV Y+LA E  L   YDD    +     H + 
Sbjct: 151 AFLVEDSASPPYHNYLNNLASKANILIVSVNYRLAPEYPLPVAYDDCMEALNWVNKHSDG 210

Query: 67  DDAKIWLNEHTNFDHVFI 85
              + W+N+H +FDH+FI
Sbjct: 211 TGQEDWINKHGDFDHLFI 228

BLAST of Lsi10G003000 vs. ExPASy Swiss-Prot
Match: Q9SMM9 (Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 3.5e-10
Identity = 29/77 (37.66%), Postives = 48/77 (62.34%), Query Frame = 0

Query: 8   FSVASAASPNYHRHLVSFAAEANIVAISVEYQLARENSLSTTYDDAWAVVEGAASHCNRD 67
           F V +A SP YH  L +  + ++ VA+SV+Y+ A E+ + T+YDD+W  ++   SH    
Sbjct: 85  FLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHPIPTSYDDSWTALKWVFSHIAGS 144

Query: 68  DAKIWLNEHTNFDHVFI 85
            ++ WLN+H +F  VF+
Sbjct: 145 GSEDWLNKHADFSKVFL 161

BLAST of Lsi10G003000 vs. ExPASy Swiss-Prot
Match: Q9FX92 (Probable carboxylesterase 3 OS=Arabidopsis thaliana OX=3702 GN=CXE3 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 5.9e-10
Identity = 32/78 (41.03%), Postives = 48/78 (61.54%), Query Frame = 0

Query: 7   AFSVASAASPNYHRHLVSFAAEANIVAISVEYQLARENSLSTTYDDAWAVVEGAASHCNR 66
           A+ + S  SP YH +L      AN +A+SV+Y+LA E+ +   YDD+W+ ++   SH   
Sbjct: 84  AYIIQSPFSPVYHNYLTEVVITANCLAVSVQYRLAPEHPVPAAYDDSWSAIQWIFSH--S 143

Query: 67  DDAKIWLNEHTNFDHVFI 85
           DD   W+NE+ +FD VFI
Sbjct: 144 DD---WINEYADFDRVFI 156

BLAST of Lsi10G003000 vs. ExPASy Swiss-Prot
Match: Q5NUF3 (2-hydroxyisoflavanone dehydratase OS=Glycine max OX=3847 GN=HIDH PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 3.2e-08
Identity = 31/81 (38.27%), Postives = 47/81 (58.02%), Query Frame = 0

Query: 7   AFSVASAASPNYHRHLVSFAAEANIVAISVEYQLARENSLSTTYDDAWAVVEGAASHCNR 66
           AF V SA S   HR+L   A+EANI+AISV+++L   + +   Y+D W  ++  ASH N 
Sbjct: 80  AFCVESAFSFFVHRYLNILASEANIIAISVDFRLLPHHPIPAAYEDGWTTLKWIASHANN 139

Query: 67  DDA---KIWLNEHTNFDHVFI 85
            +    + WL  H +F  V++
Sbjct: 140 TNTTNPEPWLLNHADFTKVYV 160

BLAST of Lsi10G003000 vs. ExPASy Swiss-Prot
Match: Q9SX78 (Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 5.5e-08
Identity = 26/79 (32.91%), Postives = 48/79 (60.76%), Query Frame = 0

Query: 7   AFSVASAASPNYHRHLVSFAAEANIVAISVEYQLARENSLSTTYDDAWAVVEGAASHCNR 66
           AF ++S + P+YH  L     +AN++A+SV Y+LA E+ L T Y+D+W  ++   +    
Sbjct: 82  AFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPLPTAYEDSWTALKNIQA---- 141

Query: 67  DDAKIWLNEHTNFDHVFIV 86
              + W+N++ + D +F+V
Sbjct: 142 -INEPWINDYADLDSLFLV 155

BLAST of Lsi10G003000 vs. ExPASy TrEMBL
Match: A0A6J1G929 (probable carboxylesterase 2 OS=Cucurbita moschata OX=3662 GN=LOC111451823 PE=4 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 2.9e-20
Identity = 51/79 (64.56%), Postives = 59/79 (74.68%), Query Frame = 0

Query: 7   AFSVASAASPNYHRHLVSFAAEANIVAISVEYQLARENSLSTTYDDAWAVVEGAASHCNR 66
           AF++ S AS  YH HL+S AAEAN VA+SVEY+LA EN L   YDDAW  VE  ASH NR
Sbjct: 83  AFAIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPENPLPAAYDDAWKAVEWVASHSNR 142

Query: 67  DDAKIWLNEHTNFDHVFIV 86
           D A++WLNEH +FD VFIV
Sbjct: 143 DGAEMWLNEHADFDRVFIV 161

BLAST of Lsi10G003000 vs. ExPASy TrEMBL
Match: A0A6J1L361 (probable carboxylesterase 2 OS=Cucurbita maxima OX=3661 GN=LOC111499978 PE=4 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 5.0e-20
Identity = 51/79 (64.56%), Postives = 59/79 (74.68%), Query Frame = 0

Query: 7   AFSVASAASPNYHRHLVSFAAEANIVAISVEYQLARENSLSTTYDDAWAVVEGAASHCNR 66
           AF++ S AS  YH HL+S AAEAN VA+SVEY+LA EN L   YDDAW  VE  ASH NR
Sbjct: 83  AFTIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPENPLPAAYDDAWTAVEWVASHSNR 142

Query: 67  DDAKIWLNEHTNFDHVFIV 86
           D A++WLNEH +FD VFIV
Sbjct: 143 DGAEMWLNEHADFDCVFIV 161

BLAST of Lsi10G003000 vs. ExPASy TrEMBL
Match: A0A6J1D5P6 (probable carboxylesterase 2 OS=Momordica charantia OX=3673 GN=LOC111017169 PE=3 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.9e-19
Identity = 50/79 (63.29%), Postives = 59/79 (74.68%), Query Frame = 0

Query: 7   AFSVASAASPNYHRHLVSFAAEANIVAISVEYQLARENSLSTTYDDAWAVVEGAASHCNR 66
           AF++ SA S  YHRHL+S AAEAN VA+SV Y+LA E+ L   YDDAW  VE AASH NR
Sbjct: 82  AFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAPEHPLPAAYDDAWTSVEWAASHANR 141

Query: 67  DDAKIWLNEHTNFDHVFIV 86
           D A+IWLN H +FD VF+V
Sbjct: 142 DGAEIWLNNHADFDRVFLV 160

BLAST of Lsi10G003000 vs. ExPASy TrEMBL
Match: A0A7J7HGG9 (Abhydrolase_3 domain-containing protein OS=Camellia sinensis OX=4442 GN=HYC85_009659 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.8e-14
Identity = 38/78 (48.72%), Postives = 54/78 (69.23%), Query Frame = 0

Query: 8   FSVASAASPNYHRHLVSFAAEANIVAISVEYQLARENSLSTTYDDAWAVVEGAASHCNRD 67
           F + +A+SP YH +L+S  AEANIVA+SV+Y+ A E+ L   YDD+WA V+  ASH   +
Sbjct: 84  FCIETASSPTYHNYLISLVAEANIVAVSVDYRRAPEHPLPVAYDDSWAAVKWVASHSTSN 143

Query: 68  DAKIWLNEHTNFDHVFIV 86
             ++WL  + +FDHVF V
Sbjct: 144 GGEVWLRHYVDFDHVFFV 161

BLAST of Lsi10G003000 vs. ExPASy TrEMBL
Match: A0A4S4EGB9 (Abhydrolase_3 domain-containing protein OS=Camellia sinensis var. sinensis OX=542762 GN=TEA_010423 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.8e-14
Identity = 38/78 (48.72%), Postives = 54/78 (69.23%), Query Frame = 0

Query: 8   FSVASAASPNYHRHLVSFAAEANIVAISVEYQLARENSLSTTYDDAWAVVEGAASHCNRD 67
           F + +A+SP YH +L+S  AEANIVA+SV+Y+ A E+ L   YDD+WA V+  ASH   +
Sbjct: 84  FCIETASSPTYHNYLISLVAEANIVAVSVDYRRAPEHPLPVAYDDSWAAVKWVASHSTSN 143

Query: 68  DAKIWLNEHTNFDHVFIV 86
             ++WL  + +FDHVF V
Sbjct: 144 GGEVWLRHYVDFDHVFFV 161

BLAST of Lsi10G003000 vs. NCBI nr
Match: XP_023532126.1 (probable carboxylesterase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 108.6 bits (270), Expect = 2.7e-20
Identity = 52/79 (65.82%), Postives = 59/79 (74.68%), Query Frame = 0

Query: 7   AFSVASAASPNYHRHLVSFAAEANIVAISVEYQLARENSLSTTYDDAWAVVEGAASHCNR 66
           AFS+ S AS  YH HL+S AAEAN VA+SVEY+LA EN L   YDDAW  VE  ASH NR
Sbjct: 83  AFSIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPENPLPAAYDDAWKAVEWVASHSNR 142

Query: 67  DDAKIWLNEHTNFDHVFIV 86
           D A++WLNEH +FD VFIV
Sbjct: 143 DGAEMWLNEHADFDRVFIV 161

BLAST of Lsi10G003000 vs. NCBI nr
Match: XP_022948164.1 (probable carboxylesterase 2 [Cucurbita moschata])

HSP 1 Score: 107.5 bits (267), Expect = 6.1e-20
Identity = 51/79 (64.56%), Postives = 59/79 (74.68%), Query Frame = 0

Query: 7   AFSVASAASPNYHRHLVSFAAEANIVAISVEYQLARENSLSTTYDDAWAVVEGAASHCNR 66
           AF++ S AS  YH HL+S AAEAN VA+SVEY+LA EN L   YDDAW  VE  ASH NR
Sbjct: 83  AFAIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPENPLPAAYDDAWKAVEWVASHSNR 142

Query: 67  DDAKIWLNEHTNFDHVFIV 86
           D A++WLNEH +FD VFIV
Sbjct: 143 DGAEMWLNEHADFDRVFIV 161

BLAST of Lsi10G003000 vs. NCBI nr
Match: KAG7035128.1 (putative carboxylesterase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 107.5 bits (267), Expect = 6.1e-20
Identity = 51/79 (64.56%), Postives = 59/79 (74.68%), Query Frame = 0

Query: 7   AFSVASAASPNYHRHLVSFAAEANIVAISVEYQLARENSLSTTYDDAWAVVEGAASHCNR 66
           AF++ S AS  YH HL+S AAEAN VA+SVEY+LA EN L   YDDAW  VE  ASH NR
Sbjct: 83  AFAIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPENPLPAAYDDAWKAVEWVASHSNR 142

Query: 67  DDAKIWLNEHTNFDHVFIV 86
           D A++WLNEH +FD VFIV
Sbjct: 143 DGAEMWLNEHADFDRVFIV 161

BLAST of Lsi10G003000 vs. NCBI nr
Match: KAG6605131.1 (putative carboxylesterase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 107.5 bits (267), Expect = 6.1e-20
Identity = 51/79 (64.56%), Postives = 59/79 (74.68%), Query Frame = 0

Query: 7   AFSVASAASPNYHRHLVSFAAEANIVAISVEYQLARENSLSTTYDDAWAVVEGAASHCNR 66
           AF++ S AS  YH HL+S AAEAN VA+SVEY+LA EN L   YDDAW  VE  ASH NR
Sbjct: 83  AFAIGSVASSIYHSHLISLAAEANAVAVSVEYRLAPENPLPAAYDDAWKAVEWVASHSNR 142

Query: 67  DDAKIWLNEHTNFDHVFIV 86
           D A++WLNEH +FD VFIV
Sbjct: 143 DGAEMWLNEHADFDRVFIV 161

BLAST of Lsi10G003000 vs. NCBI nr
Match: XP_038900484.1 (probable carboxylesterase 2 [Benincasa hispida] >XP_038900485.1 probable carboxylesterase 2 [Benincasa hispida] >XP_038900486.1 probable carboxylesterase 2 [Benincasa hispida] >XP_038900487.1 probable carboxylesterase 2 [Benincasa hispida] >XP_038900488.1 probable carboxylesterase 2 [Benincasa hispida] >XP_038900490.1 probable carboxylesterase 2 [Benincasa hispida])

HSP 1 Score: 106.7 bits (265), Expect = 1.0e-19
Identity = 49/79 (62.03%), Postives = 60/79 (75.95%), Query Frame = 0

Query: 7   AFSVASAASPNYHRHLVSFAAEANIVAISVEYQLARENSLSTTYDDAWAVVEGAASHCNR 66
           AF++ S ASP YH HL+S AAE+N VA+SVEY+LA EN L   YDDAWA VE AASH N 
Sbjct: 85  AFAIGSVASPIYHPHLISLAAESNAVAVSVEYRLAPENPLPAAYDDAWAAVEWAASHSNG 144

Query: 67  DDAKIWLNEHTNFDHVFIV 86
           D A+IWLN+H +F  VF++
Sbjct: 145 DGAEIWLNQHADFSRVFVI 163

BLAST of Lsi10G003000 vs. TAIR 10
Match: AT3G48700.1 (carboxyesterase 13 )

HSP 1 Score: 65.5 bits (158), Expect = 2.5e-11
Identity = 29/77 (37.66%), Postives = 48/77 (62.34%), Query Frame = 0

Query: 8   FSVASAASPNYHRHLVSFAAEANIVAISVEYQLARENSLSTTYDDAWAVVEGAASHCNRD 67
           F V +A SP YH  L +  + ++ VA+SV+Y+ A E+ + T+YDD+W  ++   SH    
Sbjct: 85  FLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHPIPTSYDDSWTALKWVFSHIAGS 144

Query: 68  DAKIWLNEHTNFDHVFI 85
            ++ WLN+H +F  VF+
Sbjct: 145 GSEDWLNKHADFSKVFL 161

BLAST of Lsi10G003000 vs. TAIR 10
Match: AT1G49640.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 64.7 bits (156), Expect = 4.2e-11
Identity = 32/78 (41.03%), Postives = 48/78 (61.54%), Query Frame = 0

Query: 7   AFSVASAASPNYHRHLVSFAAEANIVAISVEYQLARENSLSTTYDDAWAVVEGAASHCNR 66
           A+ + S  SP YH +L      AN +A+SV+Y+LA E+ +   YDD+W+ ++   SH   
Sbjct: 84  AYIIQSPFSPVYHNYLTEVVITANCLAVSVQYRLAPEHPVPAAYDDSWSAIQWIFSH--S 143

Query: 67  DDAKIWLNEHTNFDHVFI 85
           DD   W+NE+ +FD VFI
Sbjct: 144 DD---WINEYADFDRVFI 156

BLAST of Lsi10G003000 vs. TAIR 10
Match: AT1G47480.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 58.2 bits (139), Expect = 3.9e-09
Identity = 26/79 (32.91%), Postives = 48/79 (60.76%), Query Frame = 0

Query: 7   AFSVASAASPNYHRHLVSFAAEANIVAISVEYQLARENSLSTTYDDAWAVVEGAASHCNR 66
           AF ++S + P+YH  L     +AN++A+SV Y+LA E+ L T Y+D+W  ++   +    
Sbjct: 82  AFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPLPTAYEDSWTALKNIQA---- 141

Query: 67  DDAKIWLNEHTNFDHVFIV 86
              + W+N++ + D +F+V
Sbjct: 142 -INEPWINDYADLDSLFLV 155

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A0H5BMX59.1e-1141.03Tuliposide B-converting enzyme 1, amyloplastic OS=Tulipa gesneriana OX=13306 GN=... [more]
Q9SMM93.5e-1037.66Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1[more]
Q9FX925.9e-1041.03Probable carboxylesterase 3 OS=Arabidopsis thaliana OX=3702 GN=CXE3 PE=2 SV=1[more]
Q5NUF33.2e-0838.272-hydroxyisoflavanone dehydratase OS=Glycine max OX=3847 GN=HIDH PE=1 SV=1[more]
Q9SX785.5e-0832.91Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G9292.9e-2064.56probable carboxylesterase 2 OS=Cucurbita moschata OX=3662 GN=LOC111451823 PE=4 S... [more]
A0A6J1L3615.0e-2064.56probable carboxylesterase 2 OS=Cucurbita maxima OX=3661 GN=LOC111499978 PE=4 SV=... [more]
A0A6J1D5P61.9e-1963.29probable carboxylesterase 2 OS=Momordica charantia OX=3673 GN=LOC111017169 PE=3 ... [more]
A0A7J7HGG91.8e-1448.72Abhydrolase_3 domain-containing protein OS=Camellia sinensis OX=4442 GN=HYC85_00... [more]
A0A4S4EGB91.8e-1448.72Abhydrolase_3 domain-containing protein OS=Camellia sinensis var. sinensis OX=54... [more]
Match NameE-valueIdentityDescription
XP_023532126.12.7e-2065.82probable carboxylesterase 2 [Cucurbita pepo subsp. pepo][more]
XP_022948164.16.1e-2064.56probable carboxylesterase 2 [Cucurbita moschata][more]
KAG7035128.16.1e-2064.56putative carboxylesterase 2 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6605131.16.1e-2064.56putative carboxylesterase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038900484.11.0e-1962.03probable carboxylesterase 2 [Benincasa hispida] >XP_038900485.1 probable carboxy... [more]
Match NameE-valueIdentityDescription
AT3G48700.12.5e-1137.66carboxyesterase 13 [more]
AT1G49640.14.2e-1141.03alpha/beta-Hydrolases superfamily protein [more]
AT1G47480.13.9e-0932.91alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 6..85
e-value: 1.5E-9
score: 39.8
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 8..67
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 8..85
e-value: 9.0E-10
score: 38.8
NoneNo IPR availablePANTHERPTHR23024:SF414CARBOXYLESTERASE 13-RELATEDcoord: 3..84
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 3..84

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G003000.1Lsi10G003000.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity