Lsi10G002560 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi10G002560 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.1e-133
Identity = 329/866 (37.99%), Postives = 454/866 (52.42%), Query Frame = 0

Query: 3   GEDTRKSFTGHLYEALVQVGINTFRDTEKLNRGMQISQELCEAINGSRFFVVVFSENYAS 62
           GEDTR +FT HLY AL + GI TFRD +KL RG  I+ EL +AI  SR  V+VFSENYA 
Sbjct: 35  GEDTRFNFTDHLYSALGRRGIRTFRD-DKLRRGEAIAPELLKAIEESRSSVIVFSENYAR 94

Query: 63  SSWCLDEIVQIMSCMERGK--GQMVWPVFYHVDPSEVRKQKAIFGEAFIVHQQRFKEDLK 122
           S WCLDE+V+IM C +  K  G  V+P+FYHVDPS VRKQ+  FGEAF  + +  K+ + 
Sbjct: 95  SRWCLDELVKIMECHKDKKDPGHAVFPIFYHVDPSHVRKQEGSFGEAFAGYGENLKDKIP 154

Query: 123 R--------------------------EIVQEIWTKLNKTLLTIARNPVGIDSHIENLKS 182
           R                          EI   I+ +L    L    N VGIDSH++ +  
Sbjct: 155 RWRTALTEAANLSGWPLQDGYESNQIKEITDSIFRRLKCKRLDAGANLVGIDSHVKEMIW 214

Query: 183 TLDLGSNDVRMIGIYGLGGIGKTTIAKAVYNCIAHLFEACSFLPDIKEKCEFSRDNELTK 242
            L + S+DVRM+G+YG+GGIGKTTIAK +YN ++  FE  SFL +I+EK        ++ 
Sbjct: 215 RLHMESSDVRMVGMYGVGGIGKTTIAKVIYNELSREFEYMSFLENIREK---FNTQGVSP 274

Query: 243 LQESLLQDLLLIKMRRSINFSDEGSNMLRDRLGKKKVLVVLDDVDHSNQLEKLAGHLSWF 302
           LQ  LL D+L  +  ++IN    G++M++D L  K V +VLDDVD  +QLE L  H  W 
Sbjct: 275 LQNQLLDDILKGEGSQNINSVAHGASMIKDILSSKIVFIVLDDVDDQSQLEYLLRHREWL 334

Query: 303 GSGSRIIITTRDLHLLNKHNIKWTYEVDKLNRREALELFCWNAFNNPVPDVSLEELSNVA 362
           G GSR+IITTR+ H+L+   +   YEV  LN  EA ELF   AF   +P      LS+  
Sbjct: 335 GEGSRVIITTRNKHVLDVQKVDDLYEVKGLNFEEACELFSLYAFEQNLPKSDYRNLSHRV 394

Query: 363 VEHANGLPLALAVVGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIF 422
           V +  GLPLAL V+G  L   +  EWES+L+KL + P  ++ + LK S+DGLG  EK+IF
Sbjct: 395 VGYCQGLPLALKVLGCLLLKKTIPEWESELRKLDREPEAEILSVLKRSYDGLGRTEKSIF 454

Query: 423 LDIACFFRGYEKEDVYSFFDACDFYPHYGIGVLIKKCLIYIDCNKFQMHDLIQLMGREIV 482
           LD+ACFF+G +++ V    DACDF+   GI  L  KCLI +  N+ +MHDLIQ MG EIV
Sbjct: 455 LDVACFFKGEDRDFVSKILDACDFHAEIGIKNLNDKCLITLQYNRIRMHDLIQQMGWEIV 514

Query: 483 RQESPSNLGRRSRLWYHQDVLQVLQE---------------------NEAKAFSRMKKLR 542
           R++ P    + SRLW   D  + L                       + + AF++M +LR
Sbjct: 515 REKFPDEPNKWSRLWDTCDFERALTAYKGIKRVETISLDLSKLKRVCSNSNAFAKMTRLR 574

Query: 543 LLIIRNNVNVIGTPRCL----------------------------SNELRWFECRRSSSS 602
           LL +++++++   P  +                            S ELR+         
Sbjct: 575 LLKVQSSLDIDFEPEYIDADDKVELYDVVMKNASKMRLGRGFKFPSYELRYLRWDGYPLD 634

Query: 603 LVPSTFHFKKLVLLSISHYPIEQLRANLK-------LSLESCETLTEVGDISLMPNLIKL 662
            +PS F   KLV L +    I+QLR   K       + L     L+++ + S MPNL +L
Sbjct: 635 FLPSNFDGGKLVELHLKCSNIKQLRLGNKDLEMLKVIDLSYSRKLSQMSEFSSMPNLERL 694

Query: 663 EIINCFNL-EYFSNVGLKSKTLQHLHLKSCCKLKKFPDIVEEMECLETLRLEGTGLTE-L 722
            +  C +L +   +VG   K L  L LKSC KLK  PD + ++E LE L L      E  
Sbjct: 695 FLRGCVSLIDIHPSVG-NMKKLTTLSLKSCKKLKNLPDSIGDLESLEILDLAYCSKFEKF 754

Query: 723 PTSIEKLSGLLYLELGLCDDLVQLPSSIYVLKMLKALTLSNCFKLQEFPQPSNSINSSLY 773
           P     +  L  L+L     +  LP SI  L+ LK L LS+C K ++FP+   ++ S   
Sbjct: 755 PEKGGNMKSLTELDL-QNTAIKDLPDSIGDLESLKYLDLSDCSKFEKFPEKGGNMKSLRE 814

BLAST of Lsi10G002560 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.1e-133
Identity = 324/902 (35.92%), Postives = 480/902 (53.22%), Query Frame = 0

Query: 3   GEDTRKSFTGHLYEALVQVGINTFRDTEKLNRGMQISQELCEAINGSRFFVVVFSENYAS 62
           GEDTRK+FT HLYE L   GI TF+D ++L  G  I  ELC+AI  S+F +VVFSENYA+
Sbjct: 20  GEDTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGATIPGELCKAIEESQFAIVVFSENYAT 79

Query: 63  SSWCLDEIVQIMSCMERGKGQMVWPVFYHVDPSEVRKQKAIFGEAFIVHQQRFKEDLK-- 122
           S WCL+E+V+IM C  R K Q V P+FY VDPS VR QK  F +AF  H+ ++K+D++  
Sbjct: 80  SRWCLNELVKIMECKTRFK-QTVIPIFYDVDPSHVRNQKESFAKAFEEHETKYKDDVEGI 139

Query: 123 ----------------------------REIVQEIWTKLNKTLLTIARNPVGIDSHIENL 182
                                       R+IV +I +KL K  L+  +N VGID+H+E +
Sbjct: 140 QRWRIALNEAANLKGSCDNRDKTDADCIRQIVDQISSKLCKISLSYLQNIVGIDTHLEKI 199

Query: 183 KSTLDLGSNDVRMIGIYGLGGIGKTTIAKAVYNCI------AHLFEACSFLPDIKEKCEF 242
           +S L++G N VR++GI+G+GG+GKTTIA+A+++ +      ++ F+   FL DIKE    
Sbjct: 200 ESLLEIGINGVRIMGIWGMGGVGKTTIARAIFDTLLGRMDSSYQFDGACFLKDIKE---- 259

Query: 243 SRDNELTKLQESLLQDLLLIKMRRSINFSDEGSNMLRDRLGKKKVLVVLDDVDHSNQ-LE 302
                +  LQ +LL +LL  + + + N  ++G + +  RL  KKVL+VLDD+D+ +  LE
Sbjct: 260 -NKRGMHSLQNALLSELL--REKANYNNEEDGKHQMASRLRSKKVLIVLDDIDNKDHYLE 319

Query: 303 KLAGHLSWFGSGSRIIITTRDLHLLNKHNIKWTYEVDKLNRREALELFCWNAFNNPVPDV 362
            LAG L WFG+GSRIIITTRD HL+ K++I   YEV  L   E+++LF  +AF   VP+ 
Sbjct: 320 YLAGDLDWFGNGSRIIITTRDKHLIEKNDI--IYEVTALPDHESIQLFKQHAFGKEVPNE 379

Query: 363 SLEELSNVAVEHANGLPLALAVVGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDG 422
           + E+LS   V +A GLPLAL V G+ L N    EW+S ++ +K      + +KLK+S+DG
Sbjct: 380 NFEKLSLEVVNYAKGLPLALKVWGSLLHNLRLTEWKSAIEHMKNNSYSGIIDKLKISYDG 439

Query: 423 LGDLEKAIFLDIACFFRGYEKEDVYSFFDACDFYPHYGIGVLIKKCLIYI-DCNKFQMHD 482
           L   ++ +FLDIACF RG EK+ +    ++C     YG+ +LI K L++I + N+ QMHD
Sbjct: 440 LEPKQQEMFLDIACFLRGEEKDYILQILESCHIGAEYGLRILIDKSLVFISEYNQVQMHD 499

Query: 483 LIQLMGREIVR-QESPSNLGRRSRLWYHQDVLQVLQENEA-------------------- 542
           LIQ MG+ IV  Q+ P   G RSRLW  ++V +V+  N                      
Sbjct: 500 LIQDMGKYIVNFQKDP---GERSRLWLAKEVEEVMSNNTGTMAMEAIWVSSYSSTLRFSN 559

Query: 543 KAFSRMKKLRLLIIRNNVNVIGTPRCLSNELRWFECRRSSSSLVPSTFHFKKLVLLSISH 602
           +A   MK+LR+  +  +         L N LR F C        PSTF  K LV L + H
Sbjct: 560 QAVKNMKRLRVFNMGRSSTHYAID-YLPNNLRCFVCTNYPWESFPSTFELKMLVHLQLRH 619

Query: 603 YPIEQLRANLK-------LSLESCETLTEVGDISLMPNLIKLEIINCFNL-EYFSNVGLK 662
             +  L    K       + L   + LT   D + MPNL  + +  C NL E   ++G  
Sbjct: 620 NSLRHLWTETKHLPSLRRIDLSWSKRLTRTPDFTGMPNLEYVNLYQCSNLEEVHHSLGCC 679

Query: 663 SK---------------------TLQHLHLKSCCKLKKFPDIVEEMECLETLRLEGTGLT 722
           SK                     +L++L L+SC  L+K P+I   M+    + ++G+G+ 
Sbjct: 680 SKVIGLYLNDCKSLKRFPCVNVESLEYLGLRSCDSLEKLPEIYGRMKPEIQIHMQGSGIR 739

Query: 723 ELPTSI-EKLSGLLYLELGLCDDLVQLPSSIYVLKMLKALTLSNCFKLQEFPQPSNSI-N 782
           ELP+SI +  + +  L L    +LV LPSSI  LK L +L++S C KL+  P+    + N
Sbjct: 740 ELPSSIFQYKTHVTKLLLWNMKNLVALPSSICRLKSLVSLSVSGCSKLESLPEEIGDLDN 799

Query: 783 SSLYGDDD---IQDLSTFVER----FIWSMNFKNSSIFE----------LDFI------- 785
             ++   D   ++  S+ +       +    FK+   FE          L+++       
Sbjct: 800 LRVFDASDTLILRPPSSIIRLNKLIILMFRGFKDGVHFEFPPVAEGLHSLEYLNLSYCNL 859

BLAST of Lsi10G002560 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 1.9e-130
Identity = 324/893 (36.28%), Postives = 479/893 (53.64%), Query Frame = 0

Query: 3   GEDTRKSFTGHLYEALVQVGINTFRDTEKLNRGMQISQELCEAINGSRFFVVVFSENYAS 62
           GEDTRK+F GHL+ AL++ GI+TF D ++L RG  IS EL +AI  SRF VVVFS+NYAS
Sbjct: 20  GEDTRKTFVGHLFNALIEKGIHTFMDDKELKRGKSISSELMKAIGESRFAVVVFSKNYAS 79

Query: 63  SSWCLDEIVQIMSCMERGKGQMVWPVFYHVDPSEVRKQKAIFG------EAFIVHQQ--- 122
           S+WCL+E+V+I+   E+ +  +V PVFY VDPS VRKQ   +       EA +V  +   
Sbjct: 80  STWCLEELVKILEIHEKFE-LIVVPVFYDVDPSTVRKQNGEYAVCFTKFEANLVDDRDKV 139

Query: 123 -RFKEDLKR----------------------EIVQEIWTKLNKTLLTIARNPVGIDSHIE 182
            R++E L +                      +I+++I+ K   ++    R+ VGI+S I+
Sbjct: 140 LRWREALTKVANISGHDLRNTYNGDESKCIQQILKDIFDKFCFSISITNRDLVGIESQIK 199

Query: 183 NLKSTLDLGSNDVRMIGIYGLGGIGKTTIAKAVYNCIAHLFEACSFLPDIKEKCEFSRDN 242
            L S L +    VR++GI+G+GG+GKTT A+A++N     FE+  FL D+K   E+ + +
Sbjct: 200 KLSSLLRMDLKGVRLVGIWGMGGVGKTTAARALFNRYYQNFESACFLEDVK---EYLQHH 259

Query: 243 ELTKLQESLLQDLLLIKMRRSINFSDEGSNMLRDRLGKKKVLVVLDDVDHSNQLEKLAGH 302
            L  LQ++LL  LL ++     + ++E   +L+ RL  KKVLVVLDDV+H++QL+KL G 
Sbjct: 260 TLLYLQKTLLSKLLKVEFVDCTD-TEEMCVILKRRLCSKKVLVVLDDVNHNDQLDKLVGA 319

Query: 303 LSWFGSGSRIIITTRDLHLLNKHNIKWTYEVDKLNRREALELFCWNAFNNPVPDVSLEEL 362
             WFGSGSRI+ITTRD+ LL  H++  TYE+  L + EA+ELF  +AF    P+   +EL
Sbjct: 320 EDWFGSGSRIVITTRDMKLLKNHDVHETYEIKVLEKDEAIELFNLHAFKRSSPEKEFKEL 379

Query: 363 SNVAVEHANGLPLALAVVGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDGLGDLE 422
            N+ V++  GLPLAL V+G+ L       W S + +LK  P  ++   LK+SFDGL D E
Sbjct: 380 LNLVVDYTGGLPLALKVLGSLLYKEDLDVWISTIDRLKDNPEGEIMATLKISFDGLRDYE 439

Query: 423 KAIFLDIACFFRGYEKEDVYSFFDACDFYPHYGIGVLIKKCLIYIDCNKFQMHDLIQLMG 482
           K+IFLDIACFFRGY + D+ + F A  F+P  G+  L++K LI+I  +K QMHDL+Q MG
Sbjct: 440 KSIFLDIACFFRGYNQRDMTALFHASGFHPVLGVKTLVEKSLIFILEDKIQMHDLMQEMG 499

Query: 483 REIVRQESPSNLGRRSRLWYHQDVL--------------------QVLQENE------AK 542
           R+I  QESP       R++  +DV                     +  +E E      A+
Sbjct: 500 RQIAVQESPMR-----RIYRPEDVKDACIGDMRKEAIEGLLLTEPEQFEEGELEYMYSAE 559

Query: 543 AFSRMKKLRLLIIRNNVNVIGTP-RCLSNELRWFECRRSSSSLVPSTFHFKKLVLLSISH 602
           A  + ++LR+L+          P   L N L W E R  SS+  PS F   KLV L++  
Sbjct: 560 ALKKTRRLRILVKEYYNRGFDEPVAYLPNSLLWLEWRNYSSNSFPSNFEPSKLVYLTMKG 619

Query: 603 YPIEQLRANLK------------------------------LSLESCETLTEV-GDISLM 662
             I +L    K                              L L SC+ L EV   +  +
Sbjct: 620 SSIIELWNGAKRLAFLTTLDLSYCHKLIQTPDFRMITNLERLILSSCDALVEVHPSVGFL 679

Query: 663 PNLIKLEIINCFNLEYFSNVGLKSKTLQHLHLKSCCKLKKFPDIVEEMECLETLRLEGTG 722
            NLI L + +C +LE    + ++S+ L+ L L  C  LK FP++   M  L+ L L  TG
Sbjct: 680 KNLILLNMDHCISLERLPAI-IQSECLEVLDLNYCFNLKMFPEVERNMTHLKKLDLTSTG 739

Query: 723 LTELPTSIEKLSGLLYLELGLCDDLVQLPSSIYVLKMLKALTLSNCFKLQEFPQ---PSN 782
           + ELP SIE LS L  L++  C+ LV LPSSI+  + LK   +S C KL   P+    SN
Sbjct: 740 IRELPASIEHLSSLENLQMHSCNQLVSLPSSIWRFRNLK---ISECEKLGSLPEIHGNSN 799

Query: 783 SINSSLYGDDDIQDLSTFVERFIWSMNFKNSSIFELDFIMAFNSFRSLTHLYLGGSNIKR 803
                +     I++L T +            ++  L+F+   N  ++++ L    S+I  
Sbjct: 800 CTRELILKLVSIKELPTSI-----------GNLTSLNFLEICNC-KTISSL---SSSI-- 859

BLAST of Lsi10G002560 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 2.5e-127
Identity = 319/864 (36.92%), Postives = 446/864 (51.62%), Query Frame = 0

Query: 3   GEDTRKSFTGHLYEALVQVGINTFRDTEKLNRGMQISQELCEAINGSRFFVVVFSENYAS 62
           GEDTR +FT HLY AL + GI TFRD ++L RG  I+ EL +AI  SR  V+VFSENYA 
Sbjct: 32  GEDTRYNFTDHLYSALGRRGIRTFRD-DRLRRGEAIAPELLKAIEESRSSVIVFSENYAH 91

Query: 63  SSWCLDEIVQIMSCMERGKGQMVWPVFYHVDPSEVRKQKAIFGEAFIVHQQRFKEDLKR- 122
           S WCLDE+V+IM C ++  G  V+P+FYHVDPS VRKQ+  FGEAF  +++ +K+ + R 
Sbjct: 92  SRWCLDELVKIMEC-QKDLGHAVFPIFYHVDPSHVRKQEGSFGEAFAGYEENWKDKIPRW 151

Query: 123 --------------------------EIVQEIWTKLNKTLLTIARNPVGIDSHIENLKST 182
                                     EI   I+ +L    L +  N VGIDSH++ +   
Sbjct: 152 RTALTEAANLSGWHLLDDRYESNQIKEITNSIFRQLKCKRLDVGANLVGIDSHVKEMILR 211

Query: 183 LDLGSNDVRMIGIYGLGGIGKTTIAKAVYNCIAHLFEACSFLPDIKEKCEFSRDNELTKL 242
           L L S+DVRM+GIYG+GGIGKTTIAK +YN ++  FE  SFL +I+E    S    L  L
Sbjct: 212 LHLESSDVRMVGIYGVGGIGKTTIAKVIYNELSCEFEYMSFLENIREG---SNPQVLFHL 271

Query: 243 QESLLQDLLLIKMRRSINFSDEGSNMLRDRLGKKKVLVVLDDVDHSNQLEKLAGHLSWFG 302
           Q  LL D+L  +  ++I+     ++M++D L  ++V +VLDDVD  +QLE L GH  W G
Sbjct: 272 QNQLLGDILEGEGSQNISSVAHRASMIKDILLSRRVFIVLDDVDDLSQLEYLLGHREWLG 331

Query: 303 SGSRIIITTRDLHLLNKHNIKWTYEVDKLNRREALELFCWNAFNNPVPDVSLEELSNVAV 362
            GSR+IITTR+ H+L    +   YEV+ LN  EA ELF   AF   +P      L+   V
Sbjct: 332 EGSRVIITTRNKHVLAVQEVDDLYEVEGLNFEEACELFSLYAFKQNLPKSDYRNLTCRVV 391

Query: 363 EHANGLPLALAVVGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFL 422
            +  GLPLAL V+G+ LC  +  +WE +LKKL   P  D+H  LK S+DGL  ++K IFL
Sbjct: 392 GYCQGLPLALKVLGSLLCKKTIPQWEGELKKLDSEPKADIHKVLKRSYDGLDRIDKNIFL 451

Query: 423 DIACFFRGYEKEDVYSFFDACDFYPHYGIGVLIKKCLIYIDCNKFQMHDLIQLMGREIVR 482
           D+ACFF+G  ++ V    D CDF    GI  L   CLI +  N+  MHDLIQ MG EIVR
Sbjct: 452 DLACFFKGEGRDFVLRILDGCDFPAETGISNLNDLCLITLPYNQICMHDLIQQMGWEIVR 511

Query: 483 QESPSNLGRRSRLWYHQDVLQVLQENE---------------------AKAFSRMKKLRL 542
           +  P    + SRLW   D  + L  +E                     +  F++M KLRL
Sbjct: 512 ENFPVEPNKWSRLWDPCDFERALTADEGIKSVETMSLDLSKLKRVCSNSNVFAKMTKLRL 571

Query: 543 LIIRNNVNV--------------------------IGTP-RCLSNELRWFECRRSSSSLV 602
           L + ++ ++                          +G   +  S ELR+          +
Sbjct: 572 LKVYSSSDIDSAHGDSDEDIEEVYDVVMKDASKMQLGQSFKFPSYELRYLRWDGYPLDSL 631

Query: 603 PSTFHFKKLVLLSISHYPIEQLRANLK-------LSLESCETLTEVGDISLMPNLIKLEI 662
           P  F   KLV L +    I+QL    K       + L     L+++ + S MPNL +L +
Sbjct: 632 PLNFDGGKLVELHLKCSNIKQLWQGHKDLERLKVIDLSYSRKLSQMSEFSSMPNLERLCL 691

Query: 663 INCFNL-EYFSNVGLKSKTLQHLHLKSCCKLKKFPDIVEEMECLETLRLEGTGLTE-LPT 722
             C +L +   +VG   K L  L L+SC KLK  PD + ++E LE+L L      E  P 
Sbjct: 692 SGCVSLIDIHPSVG-NMKKLTTLSLRSCNKLKNLPDSIGDLESLESLYLSNCSKFEKFPE 751

Query: 723 SIEKLSGLLYLELGLCDDLVQLPSSIYVLKMLKALTLSNCFKLQEFPQPSNSINSSLYGD 773
               +  L  L+L     +  LP SI  L+ L++L LSNC K ++FP+   ++ S    D
Sbjct: 752 KGGNMKSLTELDL-KNTAIKDLPDSIGDLESLESLYLSNCSKFEKFPEKGGNMKSLTELD 811

BLAST of Lsi10G002560 vs. ExPASy Swiss-Prot
Match: Q9FI14 (Disease resistance protein TAO1 OS=Arabidopsis thaliana OX=3702 GN=TAO1 PE=3 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 6.0e-97
Identity = 281/872 (32.22%), Postives = 426/872 (48.85%), Query Frame = 0

Query: 3   GEDTRKSFTGHLYEALVQVGINTFRDTEKLNRGMQISQELCEAINGSRFFVVVFSENYAS 62
           GED RK    H+ +   + GI  F D E + RG  I  EL +AI GS+  +++ S NY S
Sbjct: 48  GEDVRKGLLSHIQKEFQRNGITPFIDNE-MKRGGSIGPELLQAIRGSKIAIILLSRNYGS 107

Query: 63  SSWCLDEIVQIMSCMERGKGQMVWPVFYHVDPSEVRKQKAIFGEAF--------IVHQQR 122
           S WCLDE+V+IM C E   GQ V  VFY VDPS+VRKQK  FG+ F            QR
Sbjct: 108 SKWCLDELVEIMKCREE-LGQTVMTVFYDVDPSDVRKQKGDFGKVFKKTCVGRPEEMVQR 167

Query: 123 FKE--------------------DLKREIVQEIWTKLNKTLLTIARNPVGIDSHIENLKS 182
           +K+                    D+  +I +++   L+ T        VGI++H   + S
Sbjct: 168 WKQALTSAANILGEDSRNWENEADMIIKISKDVSDVLSFTPSKDFDEFVGIEAHTTEITS 227

Query: 183 TLDLGSNDVRMIGIYGLGGIGKTTIAKAVYNCIAHLFEACSFLPDIKEKC-EFSRDNELT 242
            L L   +VRMIGI+G  GIGKTTI++ +YN + H F+  + + +IK +      D    
Sbjct: 228 LLQLDLEEVRMIGIWGPAGIGKTTISRVLYNKLFHQFQLGAIIDNIKVRYPRPCHDEYSA 287

Query: 243 KLQESLLQDLLLIKMRRSINFSDEGSNMLRDRLGKKKVLVVLDDVDHSNQLEKLAGHLSW 302
           KLQ   LQ  LL +M    +       + ++RL  KKVL+VLDDVD   QL+ +A  + W
Sbjct: 288 KLQ---LQKELLSQMINQKDMVVPHLGVAQERLKDKKVLLVLDDVDGLVQLDAMAKDVQW 347

Query: 303 FGSGSRIIITTRDLHLLNKHNIKWTYEVDKLNRREALELFCWNAFNNPVPDVSLEELSNV 362
           FG GSRII+ T+DL LL  H IK+ Y+VD     EALE+FC  AF    P V  E+++  
Sbjct: 348 FGLGSRIIVVTQDLKLLKAHGIKYIYKVDFPTSDEALEIFCMYAFGEKSPKVGFEQIART 407

Query: 363 AVEHANGLPLALAVVGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAI 422
               A  LPL L V+G+ L   SK EW   + +L+   + D+ + LK S++ L + EK +
Sbjct: 408 VTTLAGKLPLGLRVMGSYLRRMSKQEWAKSIPRLRTSLDDDIESVLKFSYNSLAEQEKDL 467

Query: 423 FLDIACFFRGYEKEDVYSFFDACDFYPHYGIGVLIKKCLIYIDCNKFQMHDLIQLMGREI 482
           FL I CFFR    E +  F          G+ +L  K L+ ++    +MH+L+  +G +I
Sbjct: 468 FLHITCFFRRERIETLEVFLAKKSVDMRQGLQILADKSLLSLNLGNIEMHNLLVQLGLDI 527

Query: 483 VRQESPSNLGRRSRLWYHQDVLQVLQEN-----------------------EAKAFSRMK 542
           VR++S    G+R  L   +D+ +VL ++                         +AF RM 
Sbjct: 528 VRKQSIHKPGKRQFLVDTEDICEVLTDDTGTRTLIGIDLELSGVIEGVINISERAFERMC 587

Query: 543 KLRLLIIRNNV-----NVIGTPRCLSN---ELRWFECRRSSSSLVPSTFHFKKLVLLSIS 602
            L+ L   +       +++  P+ LS+   +LR     R   + +P  F+ + LV +++ 
Sbjct: 588 NLQFLRFHHPYGDRCHDILYLPQGLSHISRKLRLLHWERYPLTCLPPKFNPEFLVKINMR 647

Query: 603 HYPIEQL------RANLK-LSLESCETLTEVGDISLMPNLIKLEIINCFNL-EYFSNVGL 662
              +E+L        NLK + L  C  L E+ D S   NL +L +INC +L E  S++G 
Sbjct: 648 DSMLEKLWDGNEPIRNLKWMDLSFCVNLKELPDFSTATNLQELRLINCLSLVELPSSIG- 707

Query: 663 KSKTLQHLHLKSCCKLKKFPDIVEEMECLETLRLEG-TGLTELPTSIEKLSGLLYLELGL 722
            +  L  L L  C  L K P  +  +  L+ L L   + L +LP+S   ++ L  L L  
Sbjct: 708 NATNLLELDLIDCSSLVKLPSSIGNLTNLKKLFLNRCSSLVKLPSSFGNVTSLKELNLSG 767

Query: 723 CDDLVQLPSSIYVLKMLKALTLSNCFKLQEFPQPSNSINSSLYGDDDIQDLSTFVERFIW 782
           C  L+++PSSI  +  LK +    C  L +   PS+  N++   +  + + S+ +E    
Sbjct: 768 CSSLLEIPSSIGNIVNLKKVYADGCSSLVQL--PSSIGNNTNLKELHLLNCSSLMECPSS 827

Query: 783 SMNFKN------SSIFELDFIMAFNSFRSLTHLYLGG-SNIKRLPRGMRKFKYLQFIDCR 796
            +N         S    L  + +  +  +L  LYL   S++  LP  +     L  +   
Sbjct: 828 MLNLTRLEDLNLSGCLSLVKLPSIGNVINLQSLYLSDCSSLMELPFTIENATNLDTLYLD 887

BLAST of Lsi10G002560 vs. ExPASy TrEMBL
Match: A0A5D3C5B5 (TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold70G00650 PE=4 SV=1)

HSP 1 Score: 1132.9 bits (2929), Expect = 0.0e+00
Identity = 581/748 (77.67%), Postives = 623/748 (83.29%), Query Frame = 0

Query: 3   GEDTRKSFTGHLYEALVQVGINTFRDTEKLNRGMQISQELCEAINGSRFFVVVFSENYAS 62
           GEDTRKSF GHLYEALV+ GIN FRDTE+L RGMQISQELCEAINGSRFFVVVFS+NYAS
Sbjct: 19  GEDTRKSFMGHLYEALVRAGINVFRDTEELKRGMQISQELCEAINGSRFFVVVFSKNYAS 78

Query: 63  SSWCLDEIVQIMSCMERGKGQMVWPVFYHVDPSEVRKQKAIFGEAFIVHQQRFKEDLK-- 122
           SSWCLDE+VQIMSCME GKGQMVWP+FYH+DPSEVRKQ  +FGEAFIVH++RFKEDLK  
Sbjct: 79  SSWCLDEVVQIMSCMEGGKGQMVWPLFYHIDPSEVRKQTGLFGEAFIVHKERFKEDLKKV 138

Query: 123 -----------------------------REIVQEIWTKLNKTLLTIARNPVGIDSHIEN 182
                                        +EIV EI TKLNKTLLTIA+NPVGI SHIE 
Sbjct: 139 ERWREALTKAANLSSWHLYDSSCDEATLVKEIVHEISTKLNKTLLTIAKNPVGIGSHIEK 198

Query: 183 LKSTLDLGSNDVRMIGIYGLGGIGKTTIAKAVYNCIAHLFEACSFLPDIKEKCEFSRDNE 242
           L S LDL SNDVRMIGIYGLGGIGKTT+AKAVYNCIA+ FE CSFLP+IKEKC FSRD+E
Sbjct: 199 LNSALDLESNDVRMIGIYGLGGIGKTTVAKAVYNCIAYRFEGCSFLPNIKEKCNFSRDDE 258

Query: 243 LTKLQESLLQDLLLIKMRRSINFSDEGSNMLRDRLGKKKVLVVLDDVDHSNQLEKLAGHL 302
           LTKLQESLLQD+LLIKM RSI+FSDEGSN+LR RL  KKVL+VLDDVDHS+QLEKLAGHL
Sbjct: 259 LTKLQESLLQDILLIKMHRSISFSDEGSNVLRHRLRNKKVLIVLDDVDHSDQLEKLAGHL 318

Query: 303 SWFGSGSRIIITTRDLHLLNKHNIKWTYEVDKLNRREALELFCWNAFNNPVPDVSLEELS 362
           SWFGSGSRI+ITTRD HLLNK NIKWTY+VDKLN REALELFCWNAFNNPVPDVS EELS
Sbjct: 319 SWFGSGSRIMITTRDFHLLNKLNIKWTYKVDKLNHREALELFCWNAFNNPVPDVSFEELS 378

Query: 363 NVAVEHANGLPLALAVVGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDGLGDLEK 422
           NVAVEHANGLPLALAVVG SLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFD LGDL+K
Sbjct: 379 NVAVEHANGLPLALAVVGGSLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDKLGDLQK 438

Query: 423 AIFLDIACFFRGYEKEDVYSFFDACDFYPHYGIGVLIKKCLIYIDCNKFQMHDLIQLMGR 482
            IFLDIACFFRGY  EDVYSF DACDFYP   I VLIKKCLIYI+ N+FQMHDLIQLMG+
Sbjct: 439 TIFLDIACFFRGYPTEDVYSFLDACDFYPRSEIEVLIKKCLIYIEYNRFQMHDLIQLMGK 498

Query: 483 EIVRQESPSNLGRRSRLWYHQDVLQVLQENE----------------------AKAFSRM 542
           EIVRQESPS++GRRSRLWYHQDVLQVLQE+E                      AK+FS+M
Sbjct: 499 EIVRQESPSDVGRRSRLWYHQDVLQVLQEDEATEKVEGIVLELPKAEEVVWLDAKSFSKM 558

Query: 543 KKLRLLIIRNNVNVIGTPRCLSNELRWFECRRSSSSLVPSTFHFKKLVLLSISHYPIEQL 602
            KLRLLIIRNNVNVIGTPRC SN LRWFEC RSSSSLVP TF+ K LV+L ISHYPI  L
Sbjct: 559 TKLRLLIIRNNVNVIGTPRCFSNYLRWFECHRSSSSLVPYTFNPKNLVVLVISHYPIMHL 618

Query: 603 RANLK-------LSLESCETLTEVGDISLMPNLIKLEIINCFNLEYFSNVGLKSKTLQHL 662
            ANLK       LSLE+CE LTE+GDISLMPNLIKLEIINCFNLE FS+ GLKSKTLQ L
Sbjct: 619 VANLKCFESIRQLSLENCEMLTEIGDISLMPNLIKLEIINCFNLECFSSTGLKSKTLQCL 678

Query: 663 HLKSCCKLKKFPDIVEEMECLETLRLEGTGLTELPTSIEKLSGLLYLELGLCDDLVQLPS 691
           HLKSC KLKKFPDIVEEMECL+ L LE  G+TELP SIEKLSGL +LEL LC+DL+QLPS
Sbjct: 679 HLKSCYKLKKFPDIVEEMECLQILSLEEIGVTELPKSIEKLSGLRHLELSLCEDLLQLPS 738

BLAST of Lsi10G002560 vs. ExPASy TrEMBL
Match: A0A1S4E327 (TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498360 PE=4 SV=1)

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 590/792 (74.49%), Postives = 640/792 (80.81%), Query Frame = 0

Query: 3   GEDTRKSFTGHLYEALVQVGINTFRDTEKLNRGMQISQELCEAINGSRFFVVVFSENYAS 62
           GEDTRKSF GHLYEALV+ GIN FRDTE+L RGMQISQELCEAINGSRFFVVVFS+NYAS
Sbjct: 19  GEDTRKSFMGHLYEALVRAGINVFRDTEELKRGMQISQELCEAINGSRFFVVVFSKNYAS 78

Query: 63  SSWCLDEIVQIMSCMERGKGQMVWPVFYHVDPSEVRKQKAIFGEAFIVHQQRFKEDLK-- 122
           SSWCLDE+VQIMSCME GKGQMVWP+FYH+DPSEVRKQ  +FGEAFIVH++RFKEDLK  
Sbjct: 79  SSWCLDEVVQIMSCMEGGKGQMVWPLFYHIDPSEVRKQTGLFGEAFIVHKERFKEDLKKV 138

Query: 123 -----------------------------REIVQEIWTKLNKTLLTIARNPVGIDSHIEN 182
                                        +EIV EI TKLNKTLLTIA+NPVGI SHIE 
Sbjct: 139 ERWREALTKAANLSSWHLYDSSCDEATLVKEIVHEISTKLNKTLLTIAKNPVGIGSHIEK 198

Query: 183 LKSTLDLGSNDVRMIGIYGLGGIGKTTIAKAVYNCIAHLFEACSFLPDIKEKCEFSRDNE 242
           L S LDL SNDVRMIGIYGLGGIGKTT+AKAVYNCIA+ FE CSFLP+IKEKC FSRD+E
Sbjct: 199 LNSALDLESNDVRMIGIYGLGGIGKTTVAKAVYNCIAYRFEGCSFLPNIKEKCNFSRDDE 258

Query: 243 LTKLQESLLQDLLLIKMRRSINFSDEGSNMLRDRLGKKKVLVVLDDVDHSNQLEKLAGHL 302
           LTKLQESLLQD+LLIKM RSI+FSDEGSN+LR RL  KKVL+VLDDVDHS+QLEKLAGHL
Sbjct: 259 LTKLQESLLQDILLIKMHRSISFSDEGSNVLRHRLRNKKVLIVLDDVDHSDQLEKLAGHL 318

Query: 303 SWFGSGSRIIITTRDLHLLNKHNIKWTYEVDKLNRREALELFCWNAFNNPVPDVSLEELS 362
           SWFGSGSRI+ITTRD HLLNK NIKWTY+VDKLN REALELFCWNAFNNPVPDVS EELS
Sbjct: 319 SWFGSGSRIMITTRDFHLLNKLNIKWTYKVDKLNHREALELFCWNAFNNPVPDVSFEELS 378

Query: 363 NVAVEHANGLPLALAVVGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDGLGDLEK 422
           NVAVEHANGLPLALAVVG SLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFD LGDL+K
Sbjct: 379 NVAVEHANGLPLALAVVGGSLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDKLGDLQK 438

Query: 423 AIFLDIACFFRGYEKEDVYSFFDACDFYPHYGIGVLIKKCLIYIDCNKFQMHDLIQLMGR 482
            IFLDIACFFRGY  EDVYSF DACDFYP   I VLIKKCLIYI+ N+FQMHDLIQLMG+
Sbjct: 439 TIFLDIACFFRGYPTEDVYSFLDACDFYPRSEIEVLIKKCLIYIEYNRFQMHDLIQLMGK 498

Query: 483 EIVRQESPSNLGRRSRLWYHQDVLQVLQENE----------------------AKAFSRM 542
           EIVRQESPS++GRRSRLWYHQDVLQVLQE+E                      AK+FS+M
Sbjct: 499 EIVRQESPSDVGRRSRLWYHQDVLQVLQEDEATEKVEGIVLELPKAEEVVWLDAKSFSKM 558

Query: 543 KKLRLLIIRNNVNVIGTPRCLSNELRWFECRRSSSSLVPSTFHFKKLVLLSISHYPIEQL 602
            KLRLLIIRNNVNVIGTPRC SN LRWFEC RSSSSLVP TF+ K LV+L ISHYPI  L
Sbjct: 559 TKLRLLIIRNNVNVIGTPRCFSNYLRWFECHRSSSSLVPYTFNPKNLVVLVISHYPIMHL 618

Query: 603 RANLK-------LSLESCETLTEVGDISLMPNLIKLEIINCFNLEYFSNVGLKSKTLQHL 662
            ANLK       LSLE+CE LTE+GDISLMPNLIKLEIINCFNLE FS+ GLKSKTLQ L
Sbjct: 619 VANLKCFESIRQLSLENCEMLTEIGDISLMPNLIKLEIINCFNLECFSSTGLKSKTLQCL 678

Query: 663 HLKSCCKLKKFPDIVEEMECLETLRLEGTGLTELPTSIEKLSGLLYLELGLCDDLVQLPS 722
           HLKSC KLKKFPDIVEEMECL+ L LE  G+TELP SIEKLSGL +LEL LC+DL+QLPS
Sbjct: 679 HLKSCYKLKKFPDIVEEMECLQILSLEEIGVTELPKSIEKLSGLRHLELSLCEDLLQLPS 738

Query: 723 SIYVLKMLKALTLSNCFKLQEFPQPSNSINSSLYGDDDIQDLSTFVERF--IWSMNFKNS 733
            IY LKML +L LS C KLQEFPQPSNS              ST V++F  +  +N KN 
Sbjct: 739 IIYDLKMLNSLKLSKCLKLQEFPQPSNS--------------STNVKKFLPLRLLNQKNL 796

BLAST of Lsi10G002560 vs. ExPASy TrEMBL
Match: A0A6J1D4F5 (TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111017198 PE=4 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 2.8e-254
Identity = 483/837 (57.71%), Postives = 591/837 (70.61%), Query Frame = 0

Query: 3   GEDTRKSFTGHLYEALVQVGINTFRDTEKLNRGMQISQELCEAINGSRFFVVVFSENYAS 62
           GEDTR SFT HLYEAL Q GIN F+D+E+L RG++ISQE+ EAI+GSR  VVVFS+NYAS
Sbjct: 14  GEDTRNSFTSHLYEALAQAGINAFKDSEELKRGIEISQEIREAIDGSRILVVVFSKNYAS 73

Query: 63  SSWCLDEIVQIMSCMERGKGQMVWPVFYHVDPSEVRKQKAIFGEAFIVHQQRFKEDLK-- 122
           S+WCL+E+ +IM CMER  GQMV PVFY VDPS+V+KQ   FG AF  H++ FKEDL+  
Sbjct: 74  STWCLEEL-RIMGCMERPNGQMVLPVFYDVDPSQVQKQD--FGGAFTQHEETFKEDLEKV 133

Query: 123 ---------------------------REIVQEIWTKLNKTLLTIARNPVGIDSHIENLK 182
                                      ++IVQ+I TK+N+TLLTIA+NPVGID HI+ LK
Sbjct: 134 QKWRRALTKAANISGWHLNHRSESTLIKDIVQDILTKINRTLLTIAKNPVGIDFHIQQLK 193

Query: 183 STLDLGSNDVRMIGIYGLGGIGKTTIAKAVYNCIAHLFEACSFLPDIKEKCEFSRDNELT 242
           S L L  +DVRMIGIYGLGGIGKTTIAKA+YN IA+ F+ CSFLP IKEKC  S  + L 
Sbjct: 194 SALGLELDDVRMIGIYGLGGIGKTTIAKAIYNLIANRFDGCSFLPSIKEKCGSSMHDGLV 253

Query: 243 KLQESLLQDLLLIKMRRSINFSDEGSNMLRDRLGKKKVLVVLDDVDHSNQLEKLAGHLSW 302
           KLQE LLQD+LL KM  SINF D G+N+LR+RL KKKVL+VLDDVD+   LEKLAG+  W
Sbjct: 254 KLQELLLQDILLDKMHWSINFVDRGTNILRERLRKKKVLIVLDDVDNILHLEKLAGNGDW 313

Query: 303 FGSGSRIIITTRDLHLLNKHNIKWTYEVDKLNRREALELFCWNAFNNPVPDVSLEELSNV 362
           FG GSR+IITTR+  LL+ H I WTY+VD+LN  +ALELFCWNAF  P P+V+ EELS+ 
Sbjct: 314 FGRGSRVIITTRNKQLLDAHGIYWTYKVDELNHEDALELFCWNAFKKPHPNVNFEELSDA 373

Query: 363 AVEHANGLPLALAVVGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAI 422
            V +A+G PLAL VVGASLC CSK +WES+L+KL++IP +DVH  LKLSFDGL D+EK I
Sbjct: 374 VVRYADGHPLALTVVGASLCGCSKAQWESELQKLRRIPKKDVHEMLKLSFDGLDDVEKTI 433

Query: 423 FLDIACFFRGYEKEDVYSFFDACDFYPHYGIGVLIKKCLIYID----CNKFQMHDLIQLM 482
           FLDIACFF+G E   V SF DAC FYP  GI VL KKCLIY +     + FQMHD+IQLM
Sbjct: 434 FLDIACFFKGEEVGRVMSFLDACGFYPDSGIEVLKKKCLIYSEEMFLTSTFQMHDMIQLM 493

Query: 483 GREIVRQESPSNLGRRSRLWYHQDVLQVLQENE--------------------AKAFSRM 542
           GREIVRQESP +LGRRSRLW+H+DVLQ+L ENE                     + F +M
Sbjct: 494 GREIVRQESPFDLGRRSRLWFHEDVLQLLNENEGTLSVQGIYLNMPNVEVSLQTETFLKM 553

Query: 543 KKLRLLIIRNNVNVIGTPRCLSNELRWFECRRSSSSLVPSTFHFKKLVLLSISHYPIEQL 602
           KKLRLLIIR   NVIGTP+ LSNELRW E   + SS+VPSTF   KLV+L++    I++ 
Sbjct: 554 KKLRLLIIR-EANVIGTPQYLSNELRWLEW-PAPSSMVPSTFRANKLVVLNLQRCSIKKF 613

Query: 603 RANLK-------LSLESCETLTEVGDISLMPNLIKLEIINCFNLEYFSNVGLKSKTLQHL 662
              LK       L+   C+ LTE+ DIS+MPNL+ L++  C NLE+FS+ GLKSK L+ L
Sbjct: 614 EIGLKGFENLRYLNFNGCKMLTEICDISVMPNLVSLDLQRCSNLEFFSS-GLKSKFLKDL 673

Query: 663 HLKSCCKLKKFPDIVEEMECLETLRLEGTGLTELPTSIEKLSGLLYLELGLCDDLVQLPS 722
           +L  C KLKKFPDI+EEMECL+TL L  +G+TELP SIE L+GL+ L L  C+ LV+LPS
Sbjct: 674 NLDCCYKLKKFPDILEEMECLDTLSLRASGITELPASIENLTGLIDLNLDSCEHLVRLPS 733

Query: 723 SIYVLKMLKALTLSNCFKLQEFPQPSNS-INSSLYGDDDIQDLSTFVERFIWS----MNF 775
           SIY LK ++ + L  CF+L+ FPQP NS + SS      IQD  T ++    S       
Sbjct: 734 SIYRLKNVRFIVLDGCFRLETFPQPLNSVVVSSSSEGKRIQDSMTCLDNRYHSTLAEFRL 793

BLAST of Lsi10G002560 vs. ExPASy TrEMBL
Match: A0A5A7TN33 (TMV resistance protein N-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold75G00650 PE=4 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 1.7e-219
Identity = 391/511 (76.52%), Postives = 417/511 (81.60%), Query Frame = 0

Query: 3   GEDTRKSFTGHLYEALVQVGINTFRDTEKLNRGMQISQELCEAINGSRFFVVVFSENYAS 62
           GEDTRKSF GHLYEALV+ GIN FRDTE+L RGMQISQELCEAINGSRFFVV FS+NYAS
Sbjct: 19  GEDTRKSFMGHLYEALVRAGINVFRDTEELKRGMQISQELCEAINGSRFFVVAFSKNYAS 78

Query: 63  SSWCLDEIVQIMSCMERGKGQMVWPVFYHVDPSEVRKQKAIFGEAFIVHQQRFKEDLK-- 122
           SSWCLDE+VQIMSCME GKGQMVWP+FYH+DPSEVRKQ  +FGEA+IVH++RFKEDLK  
Sbjct: 79  SSWCLDEVVQIMSCMEGGKGQMVWPLFYHIDPSEVRKQTGVFGEAYIVHKERFKEDLKKV 138

Query: 123 -----------------------------REIVQEIWTKLNKTLLTIARNPVGIDSHIEN 182
                                        +EIV EI TKLNKTLLTIA+NPVGI SHIE 
Sbjct: 139 ERWREALTKAANLSSWHLYDSSCDEATLVKEIVHEISTKLNKTLLTIAKNPVGISSHIEK 198

Query: 183 LKSTLDLGSNDVRMIGIYGLGGIGKTTIAKAVYNCIAHLFEACSFLPDIKEKCEFSRDNE 242
           L S LDL +NDVRMIGIYGLGGIGKTT+AKAVYNCIA+ FE CSFLP+IKEKC FSRD+E
Sbjct: 199 LNSALDLETNDVRMIGIYGLGGIGKTTVAKAVYNCIAYRFEGCSFLPNIKEKCNFSRDDE 258

Query: 243 LTKLQESLLQDLLLIKMRRSINFSDEGSNMLRDRLGKKKVLVVLDDVDHSNQLEKLAGHL 302
           LTKLQESLLQD+LLIKM RSI+FSDEG                       NQ EKL GHL
Sbjct: 259 LTKLQESLLQDILLIKMHRSISFSDEG-----------------------NQQEKLVGHL 318

Query: 303 SWFGSGSRIIITTRDLHLLNKHNIKWTYEVDKLNRREALELFCWNAFNNPVPDVSLEELS 362
           SWFGSGSRIIITTRD HLLNK NIKWTY+VDKLN REALELFCWNAFNNPVPDVS EELS
Sbjct: 319 SWFGSGSRIIITTRDFHLLNKLNIKWTYKVDKLNHREALELFCWNAFNNPVPDVSFEELS 378

Query: 363 NVAVEHANGLPLALAVVGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDGLGDLEK 422
           NVAVE ANGLPLAL VVG SLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFD LGDL+K
Sbjct: 379 NVAVEQANGLPLALVVVGGSLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDRLGDLQK 438

Query: 423 AIFLDIACFFRGYEKEDVYSFFDACDFYPHYGIGVLIKKCLIYIDCNKFQMHDLIQLMGR 482
            IFLDIACFFRGY  EDVYSF DACDFY    I VLIKKCLIYI+ N+FQMHDLIQLMG+
Sbjct: 439 TIFLDIACFFRGYPTEDVYSFIDACDFYTRSEIEVLIKKCLIYIEYNRFQMHDLIQLMGK 498

BLAST of Lsi10G002560 vs. ExPASy TrEMBL
Match: A0A2K1WMI4 (TIR domain-containing protein OS=Populus trichocarpa OX=3694 GN=POPTR_019G002500 PE=4 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 8.2e-182
Identity = 396/983 (40.28%), Postives = 557/983 (56.66%), Query Frame = 0

Query: 3   GEDTRKSFTGHLYEALVQVGINTFRDTEKLNRGMQISQELCEAINGSRFFVVVFSENYAS 62
           G++TR +FT HLY AL   GIN F D +KL RG  I+ +L + I  SR  +V+FSENYA 
Sbjct: 21  GQETRNTFTAHLYHALCNKGINAFID-DKLERGEHITSQLNQIIEDSRISLVIFSENYAR 80

Query: 63  SSWCLDEIVQIMSCMERGKGQMVWPVFYHVDPSEVRKQKAIFGEAFIVHQ---------- 122
           S +CLDE+V+I+ C E  KGQ+V PVFY+VDPS+V +QK  FGE+   H+          
Sbjct: 81  SIYCLDELVKILECKE-SKGQVVLPVFYNVDPSDVEEQKGSFGESLDFHETYLGINAEQL 140

Query: 123 QRFKEDLK-------------------REIVQEIWTKLNKTLLTIARNPVGIDSHIENLK 182
           ++++E L                    R+IV+E+W +LN T L +A   VG+D  IE L 
Sbjct: 141 KQWREALTKAAQLSGWHLDRGNEAVFIRKIVEEVWAQLNHTSLHVAAYQVGLDQRIEELI 200

Query: 183 STLDLGSNDVRMIGIYGLGGIGKTTIAKAVYNCIAHLFEACSFLPDIKEKCEFSRDNELT 242
             L++GS++V M+GI GLGG GKTT+AKAVYN I + FEAC FL +++   EFS+   L 
Sbjct: 201 HMLNIGSSNVCMVGICGLGGSGKTTVAKAVYNLINNQFEACCFLSNVR---EFSKRYGLV 260

Query: 243 KLQESLLQDLLLIKMRRSINFSDEGSNMLRDRLGKKKVLVVLDDVDHSNQLEKLAGHLSW 302
            LQE LL ++L  K    +   D G N+++DRL  KKVL+V+DDVDH +QL+++AG   W
Sbjct: 261 HLQEKLLFEILGDK-TLVLGSVDRGINVIKDRLRHKKVLIVIDDVDHLDQLKQIAGERDW 320

Query: 303 FGSGSRIIITTRDLHLLNKHNIKWTYEVDKLNRREALELFCWNAFNNPVPDVSLEELSNV 362
           FG GS+IIITTRD  LL  H ++    V +L   +AL LFCW+AF N  P +   E+S+ 
Sbjct: 321 FGLGSKIIITTRDERLLVFHGVERLLRVKELCCDDALMLFCWHAFRNSHPPIDYLEISDQ 380

Query: 363 AVEHANGLPLALAVVGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAI 422
            V+++ GLPLAL V+G+ L   S  EWES+L KL++IPN+ ++  LK+SFDGL   E+AI
Sbjct: 381 VVKYSKGLPLALVVLGSFLYGRSIPEWESELDKLRRIPNKQIYEVLKISFDGLEHHERAI 440

Query: 423 FLDIACFFRGYEKEDVYSFFDACDFYPHYGIGVLIKKCLIYIDCNKFQMHDLIQLMGREI 482
           FLDIACFF+G EK+ V    DACDF P  GI VL++K L+YI+ NK QMHDL+Q MGR++
Sbjct: 441 FLDIACFFKGQEKDYVIKILDACDFDPVIGIQVLMEKSLVYIENNKLQMHDLLQWMGRQV 500

Query: 483 VRQESPSNLGRRSRLWYHQDVLQVLQEN---------------------EAKAFSRMKKL 542
           V QESP+  GRRSRLW+H+D+L VL EN                      AKAF +MK+L
Sbjct: 501 VHQESPNVPGRRSRLWFHEDILHVLTENMGSYEVEGLMLDLPEAEEVHLSAKAFKKMKRL 560

Query: 543 RLLIIRNNVNVIGTPRCLSNELRWFECRRSSSSLVPSTFHFKKLVLLSISHYPIEQLRAN 602
           R+LI+ +N  VIG P  L N LRW E        +PSTFH +KLV+L++ H  I+QL   
Sbjct: 561 RILIV-HNAQVIGGPENLPNALRWLEWPGCPLESMPSTFHARKLVVLNMHHSCIKQLGEG 620

Query: 603 LK-------LSLESCETLTEVGDISLMPNLIKLEIINCFNL------------------E 662
            K       +    CE LTE  D S +PNL +L +  C +L                  E
Sbjct: 621 FKNYTTLKYIDFRDCEFLTETPDFSTIPNLERLNLEGCTSLVKIHNSVGYLAKLVFLSVE 680

Query: 663 YFSNV-----GLKSKTLQHLHLKSCCKLKKFPDIVEEMECLETLRLEGTGLTELPTSIEK 722
           + SN+       + ++LQ L L  C KL+KFPDI ++M C+E + L  T + ELP SIE 
Sbjct: 681 FCSNLKSISSHFRLRSLQTLLLTGCSKLEKFPDIEDKMACIERVSLNSTAIKELPLSIEN 740

Query: 723 LSGLLYLELGLCDDLVQLPSSIYVLKMLKALTLSNCFKLQEFPQ------PSNSINSSLY 782
           L GL  + L  C +L ++PSSIY L+ LK L L  C +L  FP+      P    N S+ 
Sbjct: 741 LVGLKVMTLSFCRNLSRIPSSIYKLQHLKRLLLDGCSELTTFPENMPEETPPFCSNISMI 800

Query: 783 GDDDIQDLSTFVERFIWSMNFKNSSIFELDFIMAFNSFRSLTHLYLGGSNIKRLPRGMRK 842
            ++DI          +  ++ +N  + E+DF+M    F +L  L L G+N   LP  + +
Sbjct: 801 ANNDIMWFP-----MLTCLDLQNCKLSEVDFLMNLVCFSTLKDLDLSGNNFVNLPTCINR 860

Query: 843 FKYLQFIDCRNCKLLEHISDIPPLLQNLDASGCDSLSKID-------------ATSFFDI 878
           F  L+ ++  NC  LE I ++P  ++ + A  C SL                 +T   D+
Sbjct: 861 FTKLRRLELANCIWLEGIPELPRSIKRIGARNCTSLESYSELVRVFMFNTEDRSTKLHDL 920

BLAST of Lsi10G002560 vs. NCBI nr
Match: XP_038902439.1 (disease resistance protein RUN1-like [Benincasa hispida])

HSP 1 Score: 1191.8 bits (3082), Expect = 0.0e+00
Identity = 604/752 (80.32%), Postives = 649/752 (86.30%), Query Frame = 0

Query: 3   GEDTRKSFTGHLYEALVQVGINTFRDTEKLNRGMQISQELCEAINGSRFFVVVFSENYAS 62
           GEDTR+SFTGHLYEALVQVGINTFRDTE+L RG +ISQELCEAI  SRFF+V+FSENYAS
Sbjct: 18  GEDTRRSFTGHLYEALVQVGINTFRDTEELKRGTEISQELCEAIKESRFFLVIFSENYAS 77

Query: 63  SSWCLDEIVQIMSCMERGKGQMVWPVFYHVDPSEVRKQKAIFGEAFIVHQQRFKEDLK-- 122
           SSWCLDEIV+IM+C+E GKGQMVWPVFYHVDPS+VRKQK +FGEAFI+H+QRFKEDLK  
Sbjct: 78  SSWCLDEIVEIMNCVESGKGQMVWPVFYHVDPSQVRKQKGVFGEAFILHKQRFKEDLKKV 137

Query: 123 -----------------------------REIVQEIWTKLNKTLLTIARNPVGIDSHIEN 182
                                        +EIV EIWTKLNK LLT+A+NPVGIDSHIEN
Sbjct: 138 ERWREALTKAANLSGWHLDNRSCDEPTLIKEIVPEIWTKLNKILLTVAKNPVGIDSHIEN 197

Query: 183 LKSTLDLGSNDVRMIGIYGLGGIGKTTIAKAVYNCIAHLFEACSFLPDIKEKCEFSRDNE 242
           LKSTL L SNDVRMIGIYGLGGIGK+TIAKA+YNCIAH FEAC+FLP+IKEKC+FSRD+E
Sbjct: 198 LKSTLALESNDVRMIGIYGLGGIGKSTIAKAIYNCIAHRFEACTFLPNIKEKCKFSRDDE 257

Query: 243 LTKLQESLLQDLLLIKMRRSINFSDEGSNMLRDRLGKKKVLVVLDDVDHSNQLEKLAGHL 302
           LT+LQESLL+D+LLIK+ R + FSDEGSNMLRDRL  KKVLVVLDDVDHSNQLEKLAGHL
Sbjct: 258 LTQLQESLLEDILLIKIHRLVKFSDEGSNMLRDRLRNKKVLVVLDDVDHSNQLEKLAGHL 317

Query: 303 SWFGSGSRIIITTRDLHLLNKHNIKWTYEVDKLNRREALELFCWNAFNNPVPDVSLEELS 362
           SWFGSGSRIIITTRDLHLLNKHNIKWTY+VDKLN REALELFCWNAFNNPVPDVS EELS
Sbjct: 318 SWFGSGSRIIITTRDLHLLNKHNIKWTYKVDKLNHREALELFCWNAFNNPVPDVSFEELS 377

Query: 363 NVAVEHANGLPLALAVVGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDGLGDLEK 422
           NVAVE+ANGLPLAL VVGASLCNCSK+EWESQLKKLKKI NQDVHN+LKLSFDGLG+LEK
Sbjct: 378 NVAVEYANGLPLALVVVGASLCNCSKIEWESQLKKLKKIQNQDVHNRLKLSFDGLGELEK 437

Query: 423 AIFLDIACFFRGYEKEDVYSFFDACDFYPHYGIGVLIKKCLIYIDCNKFQMHDLIQLMGR 482
           AIFLDIACFFRGYE EDVYSF DACDFYP YGIGVLIKKCLIYI+ N+FQMHDLIQLMGR
Sbjct: 438 AIFLDIACFFRGYETEDVYSFLDACDFYPQYGIGVLIKKCLIYIEYNRFQMHDLIQLMGR 497

Query: 483 EIVRQESPSNLGRRSRLWYHQDVLQVLQENE---------------------AKAFSRMK 542
           EIVRQESPSN+GRRSRLWYHQDVLQVLQENE                     AKAFSRMK
Sbjct: 498 EIVRQESPSNIGRRSRLWYHQDVLQVLQENEGTKKVEGIILETPKAEVVWLNAKAFSRMK 557

Query: 543 KLRLLIIRNNVN--VIGTPRCLSNELRWFECRRSSSSLVPSTFHFKKLVLLSISHYPIEQ 602
           KLRLLIIRNNVN  VIGTPRCLS ELRWFECRRSSSSLVPSTFH +KLV+  ISHYPI+Q
Sbjct: 558 KLRLLIIRNNVNVIVIGTPRCLSKELRWFECRRSSSSLVPSTFHPQKLVVFGISHYPIKQ 617

Query: 603 LRANLK-------LSLESCETLTEVGDISLMPNLIKLEIINCFNLEYFSNVGLKSKTLQH 662
           L ANLK       LSLE+CETLTEVGDISLMPNLI+LEIINCFNLE FSNVGLKSKTLQ 
Sbjct: 618 LVANLKNFESIRQLSLENCETLTEVGDISLMPNLIRLEIINCFNLECFSNVGLKSKTLQR 677

Query: 663 LHLKSCCKLKKFPDIVEEMECLETLRLEGTGLTELPTSIEKLSGLLYLELGLCDDLVQLP 694
           LHLKSC KLKKFPDIVEEMEC+E L LEGTG+TELPTSIEKLSGL +LELGLC+DLV LP
Sbjct: 678 LHLKSCYKLKKFPDIVEEMECIEILSLEGTGVTELPTSIEKLSGLRHLELGLCEDLVHLP 737

BLAST of Lsi10G002560 vs. NCBI nr
Match: TYK06530.1 (TMV resistance protein N-like [Cucumis melo var. makuwa])

HSP 1 Score: 1132.9 bits (2929), Expect = 0.0e+00
Identity = 581/748 (77.67%), Postives = 623/748 (83.29%), Query Frame = 0

Query: 3   GEDTRKSFTGHLYEALVQVGINTFRDTEKLNRGMQISQELCEAINGSRFFVVVFSENYAS 62
           GEDTRKSF GHLYEALV+ GIN FRDTE+L RGMQISQELCEAINGSRFFVVVFS+NYAS
Sbjct: 19  GEDTRKSFMGHLYEALVRAGINVFRDTEELKRGMQISQELCEAINGSRFFVVVFSKNYAS 78

Query: 63  SSWCLDEIVQIMSCMERGKGQMVWPVFYHVDPSEVRKQKAIFGEAFIVHQQRFKEDLK-- 122
           SSWCLDE+VQIMSCME GKGQMVWP+FYH+DPSEVRKQ  +FGEAFIVH++RFKEDLK  
Sbjct: 79  SSWCLDEVVQIMSCMEGGKGQMVWPLFYHIDPSEVRKQTGLFGEAFIVHKERFKEDLKKV 138

Query: 123 -----------------------------REIVQEIWTKLNKTLLTIARNPVGIDSHIEN 182
                                        +EIV EI TKLNKTLLTIA+NPVGI SHIE 
Sbjct: 139 ERWREALTKAANLSSWHLYDSSCDEATLVKEIVHEISTKLNKTLLTIAKNPVGIGSHIEK 198

Query: 183 LKSTLDLGSNDVRMIGIYGLGGIGKTTIAKAVYNCIAHLFEACSFLPDIKEKCEFSRDNE 242
           L S LDL SNDVRMIGIYGLGGIGKTT+AKAVYNCIA+ FE CSFLP+IKEKC FSRD+E
Sbjct: 199 LNSALDLESNDVRMIGIYGLGGIGKTTVAKAVYNCIAYRFEGCSFLPNIKEKCNFSRDDE 258

Query: 243 LTKLQESLLQDLLLIKMRRSINFSDEGSNMLRDRLGKKKVLVVLDDVDHSNQLEKLAGHL 302
           LTKLQESLLQD+LLIKM RSI+FSDEGSN+LR RL  KKVL+VLDDVDHS+QLEKLAGHL
Sbjct: 259 LTKLQESLLQDILLIKMHRSISFSDEGSNVLRHRLRNKKVLIVLDDVDHSDQLEKLAGHL 318

Query: 303 SWFGSGSRIIITTRDLHLLNKHNIKWTYEVDKLNRREALELFCWNAFNNPVPDVSLEELS 362
           SWFGSGSRI+ITTRD HLLNK NIKWTY+VDKLN REALELFCWNAFNNPVPDVS EELS
Sbjct: 319 SWFGSGSRIMITTRDFHLLNKLNIKWTYKVDKLNHREALELFCWNAFNNPVPDVSFEELS 378

Query: 363 NVAVEHANGLPLALAVVGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDGLGDLEK 422
           NVAVEHANGLPLALAVVG SLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFD LGDL+K
Sbjct: 379 NVAVEHANGLPLALAVVGGSLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDKLGDLQK 438

Query: 423 AIFLDIACFFRGYEKEDVYSFFDACDFYPHYGIGVLIKKCLIYIDCNKFQMHDLIQLMGR 482
            IFLDIACFFRGY  EDVYSF DACDFYP   I VLIKKCLIYI+ N+FQMHDLIQLMG+
Sbjct: 439 TIFLDIACFFRGYPTEDVYSFLDACDFYPRSEIEVLIKKCLIYIEYNRFQMHDLIQLMGK 498

Query: 483 EIVRQESPSNLGRRSRLWYHQDVLQVLQENE----------------------AKAFSRM 542
           EIVRQESPS++GRRSRLWYHQDVLQVLQE+E                      AK+FS+M
Sbjct: 499 EIVRQESPSDVGRRSRLWYHQDVLQVLQEDEATEKVEGIVLELPKAEEVVWLDAKSFSKM 558

Query: 543 KKLRLLIIRNNVNVIGTPRCLSNELRWFECRRSSSSLVPSTFHFKKLVLLSISHYPIEQL 602
            KLRLLIIRNNVNVIGTPRC SN LRWFEC RSSSSLVP TF+ K LV+L ISHYPI  L
Sbjct: 559 TKLRLLIIRNNVNVIGTPRCFSNYLRWFECHRSSSSLVPYTFNPKNLVVLVISHYPIMHL 618

Query: 603 RANLK-------LSLESCETLTEVGDISLMPNLIKLEIINCFNLEYFSNVGLKSKTLQHL 662
            ANLK       LSLE+CE LTE+GDISLMPNLIKLEIINCFNLE FS+ GLKSKTLQ L
Sbjct: 619 VANLKCFESIRQLSLENCEMLTEIGDISLMPNLIKLEIINCFNLECFSSTGLKSKTLQCL 678

Query: 663 HLKSCCKLKKFPDIVEEMECLETLRLEGTGLTELPTSIEKLSGLLYLELGLCDDLVQLPS 691
           HLKSC KLKKFPDIVEEMECL+ L LE  G+TELP SIEKLSGL +LEL LC+DL+QLPS
Sbjct: 679 HLKSCYKLKKFPDIVEEMECLQILSLEEIGVTELPKSIEKLSGLRHLELSLCEDLLQLPS 738

BLAST of Lsi10G002560 vs. NCBI nr
Match: XP_016902380.1 (PREDICTED: TMV resistance protein N-like [Cucumis melo])

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 590/792 (74.49%), Postives = 640/792 (80.81%), Query Frame = 0

Query: 3   GEDTRKSFTGHLYEALVQVGINTFRDTEKLNRGMQISQELCEAINGSRFFVVVFSENYAS 62
           GEDTRKSF GHLYEALV+ GIN FRDTE+L RGMQISQELCEAINGSRFFVVVFS+NYAS
Sbjct: 19  GEDTRKSFMGHLYEALVRAGINVFRDTEELKRGMQISQELCEAINGSRFFVVVFSKNYAS 78

Query: 63  SSWCLDEIVQIMSCMERGKGQMVWPVFYHVDPSEVRKQKAIFGEAFIVHQQRFKEDLK-- 122
           SSWCLDE+VQIMSCME GKGQMVWP+FYH+DPSEVRKQ  +FGEAFIVH++RFKEDLK  
Sbjct: 79  SSWCLDEVVQIMSCMEGGKGQMVWPLFYHIDPSEVRKQTGLFGEAFIVHKERFKEDLKKV 138

Query: 123 -----------------------------REIVQEIWTKLNKTLLTIARNPVGIDSHIEN 182
                                        +EIV EI TKLNKTLLTIA+NPVGI SHIE 
Sbjct: 139 ERWREALTKAANLSSWHLYDSSCDEATLVKEIVHEISTKLNKTLLTIAKNPVGIGSHIEK 198

Query: 183 LKSTLDLGSNDVRMIGIYGLGGIGKTTIAKAVYNCIAHLFEACSFLPDIKEKCEFSRDNE 242
           L S LDL SNDVRMIGIYGLGGIGKTT+AKAVYNCIA+ FE CSFLP+IKEKC FSRD+E
Sbjct: 199 LNSALDLESNDVRMIGIYGLGGIGKTTVAKAVYNCIAYRFEGCSFLPNIKEKCNFSRDDE 258

Query: 243 LTKLQESLLQDLLLIKMRRSINFSDEGSNMLRDRLGKKKVLVVLDDVDHSNQLEKLAGHL 302
           LTKLQESLLQD+LLIKM RSI+FSDEGSN+LR RL  KKVL+VLDDVDHS+QLEKLAGHL
Sbjct: 259 LTKLQESLLQDILLIKMHRSISFSDEGSNVLRHRLRNKKVLIVLDDVDHSDQLEKLAGHL 318

Query: 303 SWFGSGSRIIITTRDLHLLNKHNIKWTYEVDKLNRREALELFCWNAFNNPVPDVSLEELS 362
           SWFGSGSRI+ITTRD HLLNK NIKWTY+VDKLN REALELFCWNAFNNPVPDVS EELS
Sbjct: 319 SWFGSGSRIMITTRDFHLLNKLNIKWTYKVDKLNHREALELFCWNAFNNPVPDVSFEELS 378

Query: 363 NVAVEHANGLPLALAVVGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDGLGDLEK 422
           NVAVEHANGLPLALAVVG SLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFD LGDL+K
Sbjct: 379 NVAVEHANGLPLALAVVGGSLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDKLGDLQK 438

Query: 423 AIFLDIACFFRGYEKEDVYSFFDACDFYPHYGIGVLIKKCLIYIDCNKFQMHDLIQLMGR 482
            IFLDIACFFRGY  EDVYSF DACDFYP   I VLIKKCLIYI+ N+FQMHDLIQLMG+
Sbjct: 439 TIFLDIACFFRGYPTEDVYSFLDACDFYPRSEIEVLIKKCLIYIEYNRFQMHDLIQLMGK 498

Query: 483 EIVRQESPSNLGRRSRLWYHQDVLQVLQENE----------------------AKAFSRM 542
           EIVRQESPS++GRRSRLWYHQDVLQVLQE+E                      AK+FS+M
Sbjct: 499 EIVRQESPSDVGRRSRLWYHQDVLQVLQEDEATEKVEGIVLELPKAEEVVWLDAKSFSKM 558

Query: 543 KKLRLLIIRNNVNVIGTPRCLSNELRWFECRRSSSSLVPSTFHFKKLVLLSISHYPIEQL 602
            KLRLLIIRNNVNVIGTPRC SN LRWFEC RSSSSLVP TF+ K LV+L ISHYPI  L
Sbjct: 559 TKLRLLIIRNNVNVIGTPRCFSNYLRWFECHRSSSSLVPYTFNPKNLVVLVISHYPIMHL 618

Query: 603 RANLK-------LSLESCETLTEVGDISLMPNLIKLEIINCFNLEYFSNVGLKSKTLQHL 662
            ANLK       LSLE+CE LTE+GDISLMPNLIKLEIINCFNLE FS+ GLKSKTLQ L
Sbjct: 619 VANLKCFESIRQLSLENCEMLTEIGDISLMPNLIKLEIINCFNLECFSSTGLKSKTLQCL 678

Query: 663 HLKSCCKLKKFPDIVEEMECLETLRLEGTGLTELPTSIEKLSGLLYLELGLCDDLVQLPS 722
           HLKSC KLKKFPDIVEEMECL+ L LE  G+TELP SIEKLSGL +LEL LC+DL+QLPS
Sbjct: 679 HLKSCYKLKKFPDIVEEMECLQILSLEEIGVTELPKSIEKLSGLRHLELSLCEDLLQLPS 738

Query: 723 SIYVLKMLKALTLSNCFKLQEFPQPSNSINSSLYGDDDIQDLSTFVERF--IWSMNFKNS 733
            IY LKML +L LS C KLQEFPQPSNS              ST V++F  +  +N KN 
Sbjct: 739 IIYDLKMLNSLKLSKCLKLQEFPQPSNS--------------STNVKKFLPLRLLNQKNL 796

BLAST of Lsi10G002560 vs. NCBI nr
Match: XP_038901313.1 (disease resistance protein RUN1-like [Benincasa hispida])

HSP 1 Score: 958.7 bits (2477), Expect = 3.5e-275
Identity = 482/605 (79.67%), Postives = 515/605 (85.12%), Query Frame = 0

Query: 3   GEDTRKSFTGHLYEALVQVGINTFRDTEKLNRGMQISQELCEAINGSRFFVVVFSENYAS 62
           GEDTRKSFTGHLYEALVQVGINTFRDTE+L RG +ISQELCEAI GSRFF+VVFSENYAS
Sbjct: 18  GEDTRKSFTGHLYEALVQVGINTFRDTEELKRGTEISQELCEAIKGSRFFLVVFSENYAS 77

Query: 63  SSWCLDEIVQIMSCMERGKGQMVWPVFYHVDPSEVRKQKAIFGEAFIVHQQRFKEDLKR- 122
           SSWCLDE+VQIM+C+E GKGQMVWPVFYHVDPS+VRKQK  FGE FI+ ++RFKEDLKR 
Sbjct: 78  SSWCLDEVVQIMNCVESGKGQMVWPVFYHVDPSQVRKQKGTFGEPFILQKERFKEDLKRV 137

Query: 123 ------------------------------EIVQEIWTKLNKTLLTIARNPVGIDSHIEN 182
                                         EIVQEIW KLNKTLL +A+NPVGI SHIE 
Sbjct: 138 ERWREALTKVANLSGTHLDNRSSDEPTIIKEIVQEIWIKLNKTLLIVAKNPVGIGSHIEK 197

Query: 183 LKSTLDLGSNDVRMIGIYGLGGIGKTTIAKAVYNCIAHLFEACSFLPDIKEKCEFSRDNE 242
           LKSTLD+ SNDVRMIGIYGLGGIGKTTIAKA+YNCIAH FEAC+FLP+IKEKC+FSRD+E
Sbjct: 198 LKSTLDIESNDVRMIGIYGLGGIGKTTIAKAIYNCIAHRFEACTFLPNIKEKCKFSRDDE 257

Query: 243 LTKLQESLLQDLLLIKMRRSINFSDEGSNMLRDRLGKKKVLVVLDDVDHSNQLEKLAGHL 302
           LT+LQESLL+D+LLIK+ RSI FSD+GSNMLRDRL  KKVLVVLDDVDHSNQLEKL GHL
Sbjct: 258 LTQLQESLLEDILLIKIHRSIKFSDKGSNMLRDRLRNKKVLVVLDDVDHSNQLEKLTGHL 317

Query: 303 SWFGSGSRIIITTRDLHLLNKHNIKWTYEVDKLNRREALELFCWNAFNNPVPDVSLEELS 362
           SWFGSGSRIIITTRDLHLLNKHNIKWTY+VD+LN REALELFCWNAFNNPVPDVS EELS
Sbjct: 318 SWFGSGSRIIITTRDLHLLNKHNIKWTYKVDELNHREALELFCWNAFNNPVPDVSFEELS 377

Query: 363 NVAVEHANGLPLALAVVGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDGLGDLEK 422
           NVAV+HA+GLPLAL VVGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDGLG+LEK
Sbjct: 378 NVAVKHADGLPLALEVVGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDGLGELEK 437

Query: 423 AIFLDIACFFRGYEKEDVYSFFDACDFYPHYGIGVLIKKCLIYIDCNKFQMHDLIQLMGR 482
           AIFLDIACFFRGY  EDV SFFD CDFYPHYGIGVLIKK LIYID + F+MHDLIQLMGR
Sbjct: 438 AIFLDIACFFRGYSTEDVNSFFDVCDFYPHYGIGVLIKKSLIYIDRDTFEMHDLIQLMGR 497

Query: 483 EIVRQESPSNLGRRSRLWYHQDVLQVLQENE----------------------AKAFSRM 542
           EIVRQESPS+ GRRSRLWYHQDVLQ+LQENE                      AKAFSRM
Sbjct: 498 EIVRQESPSDPGRRSRLWYHQDVLQILQENEGTKKVEGIVLEIPKAEEEVWLHAKAFSRM 557

Query: 543 KKLRLLIIRNNVNVIGTPRCLSNELRWFECRRSSSSLVPSTFHFKKLVLLSISHYPIEQL 555
           KKLR LIIRNNVNVIGTPR LSNELRWFECRRSSSSLVPSTFH  KLVLL  SHYPI+QL
Sbjct: 558 KKLRSLIIRNNVNVIGTPRYLSNELRWFECRRSSSSLVPSTFHPTKLVLLGTSHYPIQQL 617

BLAST of Lsi10G002560 vs. NCBI nr
Match: XP_022148563.1 (TMV resistance protein N-like [Momordica charantia])

HSP 1 Score: 888.3 bits (2294), Expect = 5.7e-254
Identity = 483/837 (57.71%), Postives = 591/837 (70.61%), Query Frame = 0

Query: 3   GEDTRKSFTGHLYEALVQVGINTFRDTEKLNRGMQISQELCEAINGSRFFVVVFSENYAS 62
           GEDTR SFT HLYEAL Q GIN F+D+E+L RG++ISQE+ EAI+GSR  VVVFS+NYAS
Sbjct: 14  GEDTRNSFTSHLYEALAQAGINAFKDSEELKRGIEISQEIREAIDGSRILVVVFSKNYAS 73

Query: 63  SSWCLDEIVQIMSCMERGKGQMVWPVFYHVDPSEVRKQKAIFGEAFIVHQQRFKEDLK-- 122
           S+WCL+E+ +IM CMER  GQMV PVFY VDPS+V+KQ   FG AF  H++ FKEDL+  
Sbjct: 74  STWCLEEL-RIMGCMERPNGQMVLPVFYDVDPSQVQKQD--FGGAFTQHEETFKEDLEKV 133

Query: 123 ---------------------------REIVQEIWTKLNKTLLTIARNPVGIDSHIENLK 182
                                      ++IVQ+I TK+N+TLLTIA+NPVGID HI+ LK
Sbjct: 134 QKWRRALTKAANISGWHLNHRSESTLIKDIVQDILTKINRTLLTIAKNPVGIDFHIQQLK 193

Query: 183 STLDLGSNDVRMIGIYGLGGIGKTTIAKAVYNCIAHLFEACSFLPDIKEKCEFSRDNELT 242
           S L L  +DVRMIGIYGLGGIGKTTIAKA+YN IA+ F+ CSFLP IKEKC  S  + L 
Sbjct: 194 SALGLELDDVRMIGIYGLGGIGKTTIAKAIYNLIANRFDGCSFLPSIKEKCGSSMHDGLV 253

Query: 243 KLQESLLQDLLLIKMRRSINFSDEGSNMLRDRLGKKKVLVVLDDVDHSNQLEKLAGHLSW 302
           KLQE LLQD+LL KM  SINF D G+N+LR+RL KKKVL+VLDDVD+   LEKLAG+  W
Sbjct: 254 KLQELLLQDILLDKMHWSINFVDRGTNILRERLRKKKVLIVLDDVDNILHLEKLAGNGDW 313

Query: 303 FGSGSRIIITTRDLHLLNKHNIKWTYEVDKLNRREALELFCWNAFNNPVPDVSLEELSNV 362
           FG GSR+IITTR+  LL+ H I WTY+VD+LN  +ALELFCWNAF  P P+V+ EELS+ 
Sbjct: 314 FGRGSRVIITTRNKQLLDAHGIYWTYKVDELNHEDALELFCWNAFKKPHPNVNFEELSDA 373

Query: 363 AVEHANGLPLALAVVGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAI 422
            V +A+G PLAL VVGASLC CSK +WES+L+KL++IP +DVH  LKLSFDGL D+EK I
Sbjct: 374 VVRYADGHPLALTVVGASLCGCSKAQWESELQKLRRIPKKDVHEMLKLSFDGLDDVEKTI 433

Query: 423 FLDIACFFRGYEKEDVYSFFDACDFYPHYGIGVLIKKCLIYID----CNKFQMHDLIQLM 482
           FLDIACFF+G E   V SF DAC FYP  GI VL KKCLIY +     + FQMHD+IQLM
Sbjct: 434 FLDIACFFKGEEVGRVMSFLDACGFYPDSGIEVLKKKCLIYSEEMFLTSTFQMHDMIQLM 493

Query: 483 GREIVRQESPSNLGRRSRLWYHQDVLQVLQENE--------------------AKAFSRM 542
           GREIVRQESP +LGRRSRLW+H+DVLQ+L ENE                     + F +M
Sbjct: 494 GREIVRQESPFDLGRRSRLWFHEDVLQLLNENEGTLSVQGIYLNMPNVEVSLQTETFLKM 553

Query: 543 KKLRLLIIRNNVNVIGTPRCLSNELRWFECRRSSSSLVPSTFHFKKLVLLSISHYPIEQL 602
           KKLRLLIIR   NVIGTP+ LSNELRW E   + SS+VPSTF   KLV+L++    I++ 
Sbjct: 554 KKLRLLIIR-EANVIGTPQYLSNELRWLEW-PAPSSMVPSTFRANKLVVLNLQRCSIKKF 613

Query: 603 RANLK-------LSLESCETLTEVGDISLMPNLIKLEIINCFNLEYFSNVGLKSKTLQHL 662
              LK       L+   C+ LTE+ DIS+MPNL+ L++  C NLE+FS+ GLKSK L+ L
Sbjct: 614 EIGLKGFENLRYLNFNGCKMLTEICDISVMPNLVSLDLQRCSNLEFFSS-GLKSKFLKDL 673

Query: 663 HLKSCCKLKKFPDIVEEMECLETLRLEGTGLTELPTSIEKLSGLLYLELGLCDDLVQLPS 722
           +L  C KLKKFPDI+EEMECL+TL L  +G+TELP SIE L+GL+ L L  C+ LV+LPS
Sbjct: 674 NLDCCYKLKKFPDILEEMECLDTLSLRASGITELPASIENLTGLIDLNLDSCEHLVRLPS 733

Query: 723 SIYVLKMLKALTLSNCFKLQEFPQPSNS-INSSLYGDDDIQDLSTFVERFIWS----MNF 775
           SIY LK ++ + L  CF+L+ FPQP NS + SS      IQD  T ++    S       
Sbjct: 734 SIYRLKNVRFIVLDGCFRLETFPQPLNSVVVSSSSEGKRIQDSMTCLDNRYHSTLAEFRL 793

BLAST of Lsi10G002560 vs. TAIR 10
Match: AT5G36930.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 433.0 bits (1112), Expect = 6.1e-121
Identity = 309/894 (34.56%), Postives = 457/894 (51.12%), Query Frame = 0

Query: 3   GEDTRKSFTGHLYEALVQVGINTFRDTEKLNRGMQISQELCEAINGSRFFVVVFSENYAS 62
           G D RK+F  HLY++L + GI+TF D  +L RG  IS EL  AI  S+  +VV +++YAS
Sbjct: 22  GADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKILIVVLTKDYAS 81

Query: 63  SSWCLDEIVQIMSCMERGKGQMVWPVFYHVDPSEVRKQKAIFGEAFIVHQ-----QRFKE 122
           S+WCLDE+V IM   +     MV+P+F +VDPS++R Q+  + ++F  H+      + K+
Sbjct: 82  SAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKHKNSHPLNKLKD 141

Query: 123 --------------DLKR--------EIVQEIWTKLNKTLLTIARNPVGIDSHIENLKST 182
                         D+K         +I +EI  +L    L +    VG+ S ++++ S 
Sbjct: 142 WREALTKVANISGWDIKNRNEAECIADITREILKRLPCQYLHVPSYAVGLRSRLQHISSL 201

Query: 183 LDLGSNDVRMIGIYGLGGIGKTTIAKAVYNCIAHLFEACSFLPDIKEKCEFSRDNE-LTK 242
           L +GS+ VR+I IYG+GGIGKTT+AK  +N  +HLFE  SFL + +   E+S+  E  T 
Sbjct: 202 LSIGSDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENFR---EYSKKPEGRTH 261

Query: 243 LQESLLQDLLLIKMRRSINFSDEGSNMLRDRLGKKKVLVVLDDVDHSNQLEKLAGHLSWF 302
           LQ  LL D+L    R  I F     + +++R   K+VL+V+DDVD  +QL   A     F
Sbjct: 262 LQHQLLSDIL---RRNDIEFKGL-DHAVKERFRSKRVLLVVDDVDDVHQLNSAAIDRDCF 321

Query: 303 GSGSRIIITTRDLHLLNKHNIKWTYEVDKLNRREALELFCWNAFNNPVPDVSLEELSNVA 362
           G GSRIIITTR++HLL +   + +Y   +L+  E+LELF W+AF    P     + S   
Sbjct: 322 GHGSRIIITTRNMHLLKQLRAEGSYSPKELDGDESLELFSWHAFRTSEPPKEFLQHSEEV 381

Query: 363 VEHANGLPLALAVVGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIF 422
           V +  GLPLA+ V+GA L   S  EWES LK LK+IPN ++  KL++SF+ L   +K +F
Sbjct: 382 VTYCAGLPLAVEVLGAFLIERSIREWESTLKLLKRIPNDNIQAKLQISFNALTIEQKDVF 441

Query: 423 LDIACFFRGYEKEDVYSFFDACDFYPHYGIGVLIKKCLIYIDCNKFQMHDLIQLMGREIV 482
           LDIACFF G +   V    D C+ YP   + +L+++CLI I  N   MHDL++ MGR+IV
Sbjct: 442 LDIACFFIGVDSYYVACILDGCNLYPDIVLSLLMERCLITISGNNIMMHDLLRDMGRQIV 501

Query: 483 RQESPSNLGRRSRLWYHQDVLQVLQEN---------------------EAKAFSRMKKLR 542
           R+ SP   G RSRLW H DV+ VL++                      E +AF++M++LR
Sbjct: 502 REISPKKCGERSRLWSHNDVVGVLKKKSGTNAIEGLSLKADVMDFQYFEVEAFAKMQELR 561

Query: 543 LLIIRNNVNVIGTPRCLSNELRWFECRRSSSSLVPSTFHFKKLVLLSISHYPIEQL---- 602
           LL +R  V++ G+      +LRW      S    P     + L  L + +  +++     
Sbjct: 562 LLELR-YVDLNGSYEHFPKDLRWLCWHGFSLECFPINLSLESLAALDLQYSNLKRFWKAQ 621

Query: 603 ----RANL--KLSLESCETLTEVGDISLMPNLIKLEIINCFNLE-YFSNVGLKSKTLQHL 662
                AN+   L L     L E  D S  PN+ KL +INC +L     ++G+  K L  L
Sbjct: 622 SPPQPANMVKYLDLSHSVYLRETPDFSYFPNVEKLILINCKSLVLVHKSIGILDKKLVLL 681

Query: 663 HLKSCCKLKKFPDIVEEMECLETLRLEG-TGLTELPTSIEKLSGLLYLELGLCDDLVQLP 722
           +L SC +L   P+ + +++ LE+L L   + L  L  ++ +L  L  L L     L ++P
Sbjct: 682 NLSSCIELDVLPEEIYKLKSLESLFLSNCSKLERLDDALGELESLTTL-LADFTALREIP 741

Query: 723 SSIYVLKMLKALTLSNCFKLQE------FPQPSNSINSSLYGDDDIQDLSTFVERFIWSM 782
           S+I  LK LK L+L+ C  L        + + S+S+  SL     +  L+      +   
Sbjct: 742 STINQLKKLKRLSLNGCKGLLSDDIDNLYSEKSHSV--SLLRPVSLSGLTYMRILSLGYC 801

Query: 783 NFKNSSIFELDFIMAFNSFRSLTHLYLGGSNIKRLPRGMRKFKYLQFIDCRNCKLLEHIS 830
           N  +  I E        S   L  L L G++   LP        L  +   +C  L+ I 
Sbjct: 802 NLSDELIPE-----DIGSLSFLRDLDLRGNSFCNLPTDFATLPNLGELLLSDCSKLQSIL 861

BLAST of Lsi10G002560 vs. TAIR 10
Match: AT5G36930.2 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 433.0 bits (1112), Expect = 6.1e-121
Identity = 309/894 (34.56%), Postives = 457/894 (51.12%), Query Frame = 0

Query: 3   GEDTRKSFTGHLYEALVQVGINTFRDTEKLNRGMQISQELCEAINGSRFFVVVFSENYAS 62
           G D RK+F  HLY++L + GI+TF D  +L RG  IS EL  AI  S+  +VV +++YAS
Sbjct: 25  GADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKILIVVLTKDYAS 84

Query: 63  SSWCLDEIVQIMSCMERGKGQMVWPVFYHVDPSEVRKQKAIFGEAFIVHQ-----QRFKE 122
           S+WCLDE+V IM   +     MV+P+F +VDPS++R Q+  + ++F  H+      + K+
Sbjct: 85  SAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKHKNSHPLNKLKD 144

Query: 123 --------------DLKR--------EIVQEIWTKLNKTLLTIARNPVGIDSHIENLKST 182
                         D+K         +I +EI  +L    L +    VG+ S ++++ S 
Sbjct: 145 WREALTKVANISGWDIKNRNEAECIADITREILKRLPCQYLHVPSYAVGLRSRLQHISSL 204

Query: 183 LDLGSNDVRMIGIYGLGGIGKTTIAKAVYNCIAHLFEACSFLPDIKEKCEFSRDNE-LTK 242
           L +GS+ VR+I IYG+GGIGKTT+AK  +N  +HLFE  SFL + +   E+S+  E  T 
Sbjct: 205 LSIGSDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENFR---EYSKKPEGRTH 264

Query: 243 LQESLLQDLLLIKMRRSINFSDEGSNMLRDRLGKKKVLVVLDDVDHSNQLEKLAGHLSWF 302
           LQ  LL D+L    R  I F     + +++R   K+VL+V+DDVD  +QL   A     F
Sbjct: 265 LQHQLLSDIL---RRNDIEFKGL-DHAVKERFRSKRVLLVVDDVDDVHQLNSAAIDRDCF 324

Query: 303 GSGSRIIITTRDLHLLNKHNIKWTYEVDKLNRREALELFCWNAFNNPVPDVSLEELSNVA 362
           G GSRIIITTR++HLL +   + +Y   +L+  E+LELF W+AF    P     + S   
Sbjct: 325 GHGSRIIITTRNMHLLKQLRAEGSYSPKELDGDESLELFSWHAFRTSEPPKEFLQHSEEV 384

Query: 363 VEHANGLPLALAVVGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIF 422
           V +  GLPLA+ V+GA L   S  EWES LK LK+IPN ++  KL++SF+ L   +K +F
Sbjct: 385 VTYCAGLPLAVEVLGAFLIERSIREWESTLKLLKRIPNDNIQAKLQISFNALTIEQKDVF 444

Query: 423 LDIACFFRGYEKEDVYSFFDACDFYPHYGIGVLIKKCLIYIDCNKFQMHDLIQLMGREIV 482
           LDIACFF G +   V    D C+ YP   + +L+++CLI I  N   MHDL++ MGR+IV
Sbjct: 445 LDIACFFIGVDSYYVACILDGCNLYPDIVLSLLMERCLITISGNNIMMHDLLRDMGRQIV 504

Query: 483 RQESPSNLGRRSRLWYHQDVLQVLQEN---------------------EAKAFSRMKKLR 542
           R+ SP   G RSRLW H DV+ VL++                      E +AF++M++LR
Sbjct: 505 REISPKKCGERSRLWSHNDVVGVLKKKSGTNAIEGLSLKADVMDFQYFEVEAFAKMQELR 564

Query: 543 LLIIRNNVNVIGTPRCLSNELRWFECRRSSSSLVPSTFHFKKLVLLSISHYPIEQL---- 602
           LL +R  V++ G+      +LRW      S    P     + L  L + +  +++     
Sbjct: 565 LLELR-YVDLNGSYEHFPKDLRWLCWHGFSLECFPINLSLESLAALDLQYSNLKRFWKAQ 624

Query: 603 ----RANL--KLSLESCETLTEVGDISLMPNLIKLEIINCFNLE-YFSNVGLKSKTLQHL 662
                AN+   L L     L E  D S  PN+ KL +INC +L     ++G+  K L  L
Sbjct: 625 SPPQPANMVKYLDLSHSVYLRETPDFSYFPNVEKLILINCKSLVLVHKSIGILDKKLVLL 684

Query: 663 HLKSCCKLKKFPDIVEEMECLETLRLEG-TGLTELPTSIEKLSGLLYLELGLCDDLVQLP 722
           +L SC +L   P+ + +++ LE+L L   + L  L  ++ +L  L  L L     L ++P
Sbjct: 685 NLSSCIELDVLPEEIYKLKSLESLFLSNCSKLERLDDALGELESLTTL-LADFTALREIP 744

Query: 723 SSIYVLKMLKALTLSNCFKLQE------FPQPSNSINSSLYGDDDIQDLSTFVERFIWSM 782
           S+I  LK LK L+L+ C  L        + + S+S+  SL     +  L+      +   
Sbjct: 745 STINQLKKLKRLSLNGCKGLLSDDIDNLYSEKSHSV--SLLRPVSLSGLTYMRILSLGYC 804

Query: 783 NFKNSSIFELDFIMAFNSFRSLTHLYLGGSNIKRLPRGMRKFKYLQFIDCRNCKLLEHIS 830
           N  +  I E        S   L  L L G++   LP        L  +   +C  L+ I 
Sbjct: 805 NLSDELIPE-----DIGSLSFLRDLDLRGNSFCNLPTDFATLPNLGELLLSDCSKLQSIL 864

BLAST of Lsi10G002560 vs. TAIR 10
Match: AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 420.6 bits (1080), Expect = 3.2e-117
Identity = 301/904 (33.30%), Postives = 453/904 (50.11%), Query Frame = 0

Query: 3   GEDTRKSFTGHLYEALVQVGINTFRDTEKLNRGMQISQELCEAINGSRFFVVVFSENYAS 62
           GED RK+F  HL+    ++GI  FRD   L RG  IS EL +AI GSRF +VV S NYA+
Sbjct: 26  GEDVRKTFVSHLFCEFDRMGIKAFRDDLDLQRGKSISPELIDAIKGSRFAIVVVSRNYAA 85

Query: 63  SSWCLDEIVQIMSCMERGKGQMVWPVFYHVDPSEVRKQKAIFGEAFIVHQQ-----RFKE 122
           SSWCLDE+++IM C +      + P+FY VDPS+VR+Q+  FGE    H       ++KE
Sbjct: 86  SSWCLDELLKIMECNK----DTIVPIFYEVDPSDVRRQRGSFGEDVESHSDKEKVGKWKE 145

Query: 123 DLK-------------------REIVQEIWTKLNKTLLTIARNPVGIDSHIENLKSTLDL 182
            LK                   ++IV++I  KL  T    ++  +G+ SH++ L+S + +
Sbjct: 146 ALKKLAAISGEDSRNWDDSKLIKKIVKDISDKLVSTSWDDSKGLIGMSSHMDFLQSMISI 205

Query: 183 GSNDVRMIGIYGLGGIGKTTIAKAVYNCIAHLFEACSFLPDIKEKCEFSRDNELTKLQES 242
              DVRM+GI+G+GG+GKTTIAK +YN ++  F+   F+ ++KE C       + +LQ  
Sbjct: 206 VDKDVRMLGIWGMGGVGKTTIAKYLYNQLSGQFQVHCFMENVKEVC---NRYGVRRLQVE 265

Query: 243 LLQDLLLIKMRRSINFSDEGSNMLRDRLGKKKVLVVLDDVDHSNQLEKLAGHLSWFGSGS 302
            L  +   + + + + S    N++++R   K V +VLDDVD S QL +L     WFG GS
Sbjct: 266 FLCRMFQERDKEAWS-SVSCCNIIKERFRHKMVFIVLDDVDRSEQLNELVKETGWFGPGS 325

Query: 303 RIIITTRDLHLLNKHNIKWTYEVDKLNRREALELFCWNAFNNP-VPDVSLEELSNVAVEH 362
           RII+TTRD HLL  H I   Y+V  L ++EAL+LFC  AF    +     EELS  AV +
Sbjct: 326 RIIVTTRDRHLLLSHGINLVYKVKCLPKKEALQLFCNYAFREEIILPHGFEELSVQAVNY 385

Query: 363 ANGLPLALAVVGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAIFLDI 422
           A+GLPLAL V+G+ L   S++EWES L +LK  P+ D+   L++S+DGL + EKAIFL I
Sbjct: 386 ASGLPLALRVLGSFLYRRSQIEWESTLARLKTYPHSDIMEVLRVSYDGLDEQEKAIFLYI 445

Query: 423 ACFFRGYEKEDVYSFFDACDFYPHYGIGVLIKKCLIYIDCNKFQMHDLIQLMGREIVRQE 482
           +CF+   + + V    D C +    GI +L +K LI       ++HDL++ MGRE+VRQ+
Sbjct: 446 SCFYNMKQVDYVRKLLDLCGYAAEIGITILTEKSLIVESNGCVKIHDLLEQMGRELVRQQ 505

Query: 483 SPSNLGRRSRLWYHQDVLQVLQENEA---------------------KAFSRMKKLRLLI 542
           + +N  +R  LW  +D+  +L EN                       +AF  +  L+LL 
Sbjct: 506 AVNNPAQRLLLWDPEDICHLLSENSGTQLVEGISLNLSEISEVFASDRAFEGLSNLKLLN 565

Query: 543 I-------RNNVNVIGTPRCLSNELRWFECRRSSSSLVPSTFHFKKLVLLSISHYPIEQ- 602
                      V++      L  +LR+          +PS F  + LV L +S+  +E+ 
Sbjct: 566 FYDLSFDGETRVHLPNGLSYLPRKLRYLRWDGYPLKTMPSRFFPEFLVELCMSNSNLEKL 625

Query: 603 ------LRANLKLSLESCETLTEVGDISLMPNLIKLEIINCFNLE--------------- 662
                 LR   K+ L  C+ L EV D+S   NL +L +  C +L                
Sbjct: 626 WDGIQPLRNLKKMDLSRCKYLVEVPDLSKATNLEELNLSYCQSLVEVTPSIKNLKGLSCF 685

Query: 663 YFSN--------VGLKSKTLQHLHLKSCCKLKKFPDIVEEMECLETLRLEGTGLTELPTS 722
           Y +N        +G+  K+L+ + +  C  LK FP+I         L L  T + ELP+S
Sbjct: 686 YLTNCIQLKDIPIGIILKSLETVGMSGCSSLKHFPEISWN---TRRLYLSSTKIEELPSS 745

Query: 723 IEKLSGLLYLELGLCDDLVQLPSSIYVLKMLKALTLSNCFKLQEFPQPSNSINSSLYGDD 782
           I +LS L+ L++  C  L  LPS +  L  LK+L L  C +L+  P             D
Sbjct: 746 ISRLSCLVKLDMSDCQRLRTLPSYLGHLVSLKSLNLDGCRRLENLP-------------D 805

Query: 783 DIQDLSTFVERFIWSMNFKNSSIFELDFIMAFNSF----RSLTHLYLGGSNIKRLPRGMR 808
            +Q+L++                 E+   +  N F     S+  L +  ++I+ +P  + 
Sbjct: 806 TLQNLTSL-------------ETLEVSGCLNVNEFPRVSTSIEVLRISETSIEEIPARIC 865

BLAST of Lsi10G002560 vs. TAIR 10
Match: AT5G18350.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 367.1 bits (941), Expect = 4.1e-101
Identity = 307/927 (33.12%), Postives = 432/927 (46.60%), Query Frame = 0

Query: 3   GEDTRKSFTGHLYEALVQVGINTFRDTEKLNRGMQISQELCEAINGSRFFVVVFSENYAS 62
           GED RK F  H+ +   + GI  F DT K+ RG  I   L +AI  S+  +V+ S+NYAS
Sbjct: 28  GEDVRKGFLSHIQKEFERKGIFPFVDT-KMKRGSSIGPVLSDAIIVSKIAIVLLSKNYAS 87

Query: 63  SSWCLDEIVQIMSCMERGKGQMVWPVFYHVDPSEVRKQKAIFGEAF-------------- 122
           S+WCL+E+V IM C E   GQ V  VFY VDPS+VRKQ   FG AF              
Sbjct: 88  STWCLNELVNIMKCREE-FGQTVMTVFYEVDPSDVRKQTGDFGIAFETTCVGKTEEVKQS 147

Query: 123 -------------IVHQQRFKE-DLKREIVQEIWTKLNKTLLTIARNPVGIDSHIENLKS 182
                         V++   KE DL  +I +++  +LN T+       VGI  H+  +KS
Sbjct: 148 WRQALIDVSNIVGEVYRIWSKESDLIDKIAEDVLDELNYTMSRDFDGYVGIGRHMRKMKS 207

Query: 183 TLDLGSNDVRMIGIYGLGGIGKTTIAKAVYNCIAHLFEACSFLPDIK----EKCE----- 242
            L L S DVRMIGI G  GIGKTTIA+A+ + I+  F+  +F+ DI+     +C      
Sbjct: 208 LLCLESGDVRMIGIVGPPGIGKTTIARALRDQISENFQLTAFIDDIRLTYPRRCYGESGL 267

Query: 243 ------FSRDNELTKLQESLLQDLLLIKMRRSINFSDEGSNMLRDRLGKKKVLVVLDDVD 302
                  + D     LQ + L ++L  K    I+  +   N L+DR    KVLV+LDDVD
Sbjct: 268 KPPTAFMNDDRRKIVLQTNFLSEILNQK-DIVIHNLNAAPNWLKDR----KVLVILDDVD 327

Query: 303 HSNQLEKLAGHLSWFGSGSRIIITTRDLHLLNKHNIKWTYEVDKLNRREALELFCWNAFN 362
           H  QL+ +A    WFG GSRIIITT+D  LL  HNI + YEV    + +AL++FC +AF 
Sbjct: 328 HLEQLDAMAKETGWFGYGSRIIITTQDRKLLKAHNIDYIYEVGLPRKDDALQIFCLSAFG 387

Query: 363 NPVPDVSLEELSNVAVEHANGLPLALAVVGASLCNCSKLEWESQLKKLKKIPNQDVHNKL 422
              P    + L+    + A  LPL L V+G+ L   S  EW++ L +LK   + D+   L
Sbjct: 388 QNFPHDDFQYLACEVTQLAGELPLGLKVLGSYLKGMSLEEWKNALPRLKTCLDGDIEKTL 447

Query: 423 KLSFDGLGDLEKAIFLDIACFFRGYEKEDVYSFFDACDFYPHYGIGVLIKKCLIYIDCNK 482
           + S+D L   ++A+FL IAC FRGYE   V  +    D    +G+ VL +K LI ID   
Sbjct: 448 RYSYDALSRKDQALFLHIACLFRGYEVGHVKQWLGKSDLDVDHGLDVLRQKSLISIDMGF 507

Query: 483 FQMHDLIQLMGREIVRQESPSNLGRRSRLWYHQDVLQVLQENEA---------------- 542
             MH L+Q +G EIVR +S      R  L    D+  V   N A                
Sbjct: 508 LNMHSLLQQLGVEIVRNQSSQEPRERQFLVDVNDISDVFTYNTAGTKSILGIRLNVPEIE 567

Query: 543 -------KAFSRMKKLRLLIIRNNV-NVIGTPR---CLSNELRWFECRRSSSSLVPSTFH 602
                    F  M  L+ L +     + +  PR   CL  +LR          L PS F 
Sbjct: 568 EKIVIDELVFDGMTNLQFLFVNEGFGDKLSLPRGLNCLPGKLRVLHWNYCPLRLWPSKFS 627

Query: 603 FKKLVLLSISHYPIEQ-------LRANLKLSLESCETLTEVGDISLMPNLIKLEIINCFN 662
              LV L +     E+       L++  ++ L   + L E+ D+S   NL +L++ +C  
Sbjct: 628 ANFLVELVMRGNNFEKLWEKILPLKSLKRMDLSHSKDLKEIPDLSNATNLEELDLSSCSG 687

Query: 663 -LEYFSNVGLKSKTLQHLHLKSCCKLKKFPDIVEEMECLETLRL-EGTGLTELPTSIEKL 722
            LE   ++G K+  L+ L L  C  LKK P  + +   L+ L L       ELP SI KL
Sbjct: 688 LLELTDSIG-KATNLKRLKLACCSLLKKLPSSIGDATNLQVLDLFHCESFEELPKSIGKL 747

Query: 723 SGLLYLELGLCDDLVQLPSSIYVLKMLKALTLSNCFKLQEFPQPSNSINSSLYGDDDIQD 782
           + L  LEL  C  LV LP+SI   K L  L++S C  LQ FP   N         +D   
Sbjct: 748 TNLKVLELMRCYKLVTLPNSIKTPK-LPVLSMSECEDLQAFPTYINL--------EDCTQ 807

Query: 783 LSTF--VERFIWSMNFKNSSI-------------FELDFIMAFNSFR------SLTHLYL 828
           L  F  +   +  ++ +N++I             + LD     N         S+  L L
Sbjct: 808 LKMFPEISTNVKELDLRNTAIENVPSSICSWSCLYRLDMSECRNLKEFPNVPVSIVELDL 867

BLAST of Lsi10G002560 vs. TAIR 10
Match: AT5G41750.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 360.9 bits (925), Expect = 3.0e-99
Identity = 292/924 (31.60%), Postives = 449/924 (48.59%), Query Frame = 0

Query: 3   GEDTRKSFTGHLYEALVQVGINTFRDTEKLNRGMQISQELCEAINGSRFFVVVFSENYAS 62
           G D RK F  HL+      GI TF D +K++RG  I  EL + I  +R  +VV S+ YAS
Sbjct: 21  GPDVRKGFLSHLHSVFASKGITTFND-QKIDRGQTIGPELIQGIREARVSIVVLSKKYAS 80

Query: 63  SSWCLDEIVQIMSCMERGKGQMVWPVFYHVDPSEVRKQKAIFGEAFIVHQQRFKEDLK-- 122
           SSWCLDE+V+I+ C E   GQ+V  VFY VDPS+V+KQ  +FGEAF    Q   E++K  
Sbjct: 81  SSWCLDELVEILKCKE-ALGQIVMTVFYEVDPSDVKKQSGVFGEAFEKTCQGKNEEVKIR 140

Query: 123 --------------------------REIVQEIWTKLNKTLLTIARNPVGIDSHIENLKS 182
                                     ++IV ++  KLN T        VG+++H++ L S
Sbjct: 141 WRNALAHVATIAGEHSLNWDNEAKMIQKIVTDVSDKLNLTPSRDFEGMVGMEAHLKRLNS 200

Query: 183 TLDLGSNDVRMIGIYGLGGIGKTTIAKAVYNCIAHLFEACSFLPDIKEKCEFSRDNELTK 242
            L L S++V+MIGI+G  GIGKTTIA+ ++N I+ +F    F+ ++K   +   ++  +K
Sbjct: 201 LLCLESDEVKMIGIWGPAGIGKTTIARTLFNKISSIFPFKCFMENLKGSIKGGAEH-YSK 260

Query: 243 LQESLLQDLLLIKMRRSINFSDEGSNMLRDRLGKKKVLVVLDDVDHSNQLEKLAGHLSWF 302
           L    LQ  LL ++ +  N        ++  L  +KVL++LDDVD   QLE LA   SWF
Sbjct: 261 LS---LQKQLLSEILKQENMKIHHLGTIKQWLHDQKVLIILDDVDDLEQLEVLAEDPSWF 320

Query: 303 GSGSRIIITTRDLHLLNKHNIKWTYEVDKLNRREALELFCWNAF-NNPVPDVSLEELSNV 362
           GSGSRII+TT D ++L  H I+  Y VD  +  EALE+ C +AF  + +PD   EEL+N 
Sbjct: 321 GSGSRIIVTTEDKNILKAHRIQDIYHVDFPSEEEALEILCLSAFKQSSIPD-GFEELANK 380

Query: 363 AVEHANGLPLALAVVGASLCNCSKLEWESQLKKLKKIPNQDVHNKLKLSFDGLGDLEKAI 422
             E    LPL L VVGASL   SK EWE  L +++   ++++ N L++ +D L   ++++
Sbjct: 381 VAELCGNLPLGLCVVGASLRRKSKNEWERLLSRIESSLDKNIDNILRIGYDRLSTEDQSL 440

Query: 423 FLDIACFFRGYEKEDVYSFFDACDFYPHYGIGVLIKKCLIYI--DCNKFQMHDLIQLMGR 482
           FL IACFF   + + + +           G  +L  + L+ I  D +    H L+Q +GR
Sbjct: 441 FLHIACFFNNEKVDYLTALLADRKLDVVNGFNILADRSLVRISTDGHVVMHHYLLQKLGR 500

Query: 483 EIVRQESPSNLGRRSRLWYHQDVLQVLQENEAK---------------------AFSRMK 542
            IV ++ P+  G+R  L   +++  VL +                         AF  M+
Sbjct: 501 RIVHEQWPNEPGKRQFLIEAEEIRDVLTKGTGTESVKGISFDTSNIEEVSVGKGAFEGMR 560

Query: 543 KLRLL-IIRNNVNVIGTPRCLSNELRWFECRRSSSSLVPSTFHFKKLV-LLSISHYPIEQ 602
            L+ L I R++ N  GT +                  +P    +   V LL   +YP + 
Sbjct: 561 NLQFLRIYRDSFNSEGTLQ------------------IPEDMEYIPPVRLLHWQNYPRKS 620

Query: 603 L--RAN----LKLSLESCETLTEVGDISLMPNLIKLEIINCFNLEYFSNVGLKSKTLQHL 662
           L  R N    +K+ + S +     G I  +PNL  +++   ++L+   N+  K+  L+ L
Sbjct: 621 LPQRFNPEHLVKIRMPSSKLKKLWGGIQPLPNLKSIDMSFSYSLKEIPNLS-KATNLEIL 680

Query: 663 HLKSCCKLKKFPDIVEEMECLETLRLEGTGLTELPTSIEKLSGLLYLELGLCDDLVQLPS 722
            L+ C                         L ELP SI  L  L  L +  C  L  +P+
Sbjct: 681 SLEFC-----------------------KSLVELPFSILNLHKLEILNVENCSMLKVIPT 740

Query: 723 SIYVLKMLKALTLSNCFKLQEFPQPSNSINSSLYGDDDIQDLSTFVERFIWSMNFKNSSI 782
           +I  L  L+ L ++ C +L+ FP  S++I     GD  I+D+   V    WS        
Sbjct: 741 NIN-LASLERLDMTGCSELRTFPDISSNIKKLNLGDTMIEDVPPSVG--CWS-------- 800

Query: 783 FELDFI-MAFNSFRSL------THLYLGGSNIKRLPRGMRKFKYLQFIDCRNCKLLEHIS 842
             LD + +   S + L      T L L  SNI+ +P  +     L +++  +C+ L+ I 
Sbjct: 801 -RLDHLYIGSRSLKRLHVPPCITSLVLWKSNIESIPESIIGLTRLDWLNVNSCRKLKSIL 860

Query: 843 DIPPLLQNLDASGCDSLSKIDATSFFDIDKLS-KNADYLCQDWTEVFLYQGICNNIGVES 858
            +P  LQ+LDA+ C SL ++  +    I  LS  N   L ++  +  + Q +   I    
Sbjct: 861 GLPSSLQDLDANDCVSLKRVCFSFHNPIRALSFNNCLNLDEEARKGIIQQSVYRYI---- 879