Lsi10G002350 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G002350
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionNB-ARC domain-containing disease resistance protein, putative
Locationchr10: 3843673 .. 3844603 (-)
RNA-Seq ExpressionLsi10G002350
SyntenyLsi10G002350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGGTTTCATTTCCTGAATTGAATGATTTATCAATTATCGGAGGTGACAATTTGGAGACGTTATGGCATACAAATATGAAACCAACTGCAAATTCCTTTTGCAAACTCCAATCAATAAGAATTGAAATGTGCACCCAATTAAGATGCATATTTCCTTCGAATATAGTGACATCACTTGTTTCTTTAGATAAACTAGAAATTATTTCTTGTGCGTCATTAAAAAGGATATTTAAAATTGAAAACCAGAGTATTGGTGACACAAAGGTAGTGTGGTCATTGAGAGAGTTACATTTACTTAATTTGCCAAATCTAAAAACCGTATGGAGAAAAAATGTCCTTAAATTTTTAACATTTCCGAGTATAAAGAGAGTGCAAATTCGTGGTTGTACTAAGCTAACACACGTGTGGAAAGAGAGCATTAAAATGACTACAAACTTTAATACCTTAGAGAGAATTGATGTAGAAAGATGTGAGAAATTGAAGTATTTATTGCCATCATCAATCACATTCTTGAACTTGAATGAGCTTCACATCGAGAAATGCAATGGAATGGTGAATTTGTTTAGCTCTAAAGTGGCAAAAAGGCTGGTGAATCTGAGCTCCATTAAAGTATCTTCTTGTAAAGCAATGAGTTGCATAGTTGCAGCAGAAGAAGAAATTGTTGAAGTTATTATTTTCAATAAATTGGGTAAGTTGGAATTGGATTGTTTACCACGATTGACAAGCTTTTACTCTGGAAAATGCATGCTTGAGTTTATAAGTTTGGAGAGTTTGGTCATAAAAAGATGCCCTCAAATGGAGACATTTTCATATGGAGTAATAATCACACCGAGATTGCATTCTATGTGGATGGAAGATACAGAATTTGGAGTATCGTGGCCAACAAGTGGTGTAAATCAAATCATCAAGAACTTCCCAAACCGAGTG

mRNA sequence

ATGGCAGGTGACAATTTGGAGACGTTATGGCATACAAATATGAAACCAACTGCAAATTCCTTTTGCAAACTCCAATCAATAAGAATTGAAATGTGCACCCAATTAAGATGCATATTTCCTTCGAATATAGTGACATCACTTGTTTCTTTAGATAAACTAGAAATTATTTCTTGTGCGTCATTAAAAAGGATATTTAAAATTGAAAACCAGAGTATTGGTGACACAAAGGTAGTGTGGTCATTGAGAGAGTTACATTTACTTAATTTGCCAAATCTAAAAACCGTATGGAGAAAAAATGTCCTTAAATTTTTAACATTTCCGAGTATAAAGAGAGTGCAAATTCGTGGTTGTACTAAGCTAACACACGTGTGGAAAGAGAGCATTAAAATGACTACAAACTTTAATACCTTAGAGAGAATTGATGTAGAAAGATGTGAGAAATTGAAGTATTTATTGCCATCATCAATCACATTCTTGAACTTGAATGAGCTTCACATCGAGAAATGCAATGGAATGGTGAATTTGTTTAGCTCTAAAGTGGCAAAAAGGCTGGTGAATCTGAGCTCCATTAAAGTATCTTCTTGTAAAGCAATGAGTTGCATAGTTGCAGCAGAAGAAGAAATTGTTGAAGTTATTATTTTCAATAAATTGGGTAAGTTGGAATTGGATTGTTTACCACGATTGACAAGCTTTTACTCTGGAAAATGCATGCTTGAGTTTATAAGTTTGGAGAGTTTGGTCATAAAAAGATGCCCTCAAATGGAGACATTTTCATATGGAGTAATAATCACACCGAGATTGCATTCTATGTGGATGGAAGATACAGAATTTGGAGTATCGTGGCCAACAAGTGGTGTAAATCAAATCATCAAGAACTTCCCAAACCGAGTG

Coding sequence (CDS)

ATGGCAGGTGACAATTTGGAGACGTTATGGCATACAAATATGAAACCAACTGCAAATTCCTTTTGCAAACTCCAATCAATAAGAATTGAAATGTGCACCCAATTAAGATGCATATTTCCTTCGAATATAGTGACATCACTTGTTTCTTTAGATAAACTAGAAATTATTTCTTGTGCGTCATTAAAAAGGATATTTAAAATTGAAAACCAGAGTATTGGTGACACAAAGGTAGTGTGGTCATTGAGAGAGTTACATTTACTTAATTTGCCAAATCTAAAAACCGTATGGAGAAAAAATGTCCTTAAATTTTTAACATTTCCGAGTATAAAGAGAGTGCAAATTCGTGGTTGTACTAAGCTAACACACGTGTGGAAAGAGAGCATTAAAATGACTACAAACTTTAATACCTTAGAGAGAATTGATGTAGAAAGATGTGAGAAATTGAAGTATTTATTGCCATCATCAATCACATTCTTGAACTTGAATGAGCTTCACATCGAGAAATGCAATGGAATGGTGAATTTGTTTAGCTCTAAAGTGGCAAAAAGGCTGGTGAATCTGAGCTCCATTAAAGTATCTTCTTGTAAAGCAATGAGTTGCATAGTTGCAGCAGAAGAAGAAATTGTTGAAGTTATTATTTTCAATAAATTGGGTAAGTTGGAATTGGATTGTTTACCACGATTGACAAGCTTTTACTCTGGAAAATGCATGCTTGAGTTTATAAGTTTGGAGAGTTTGGTCATAAAAAGATGCCCTCAAATGGAGACATTTTCATATGGAGTAATAATCACACCGAGATTGCATTCTATGTGGATGGAAGATACAGAATTTGGAGTATCGTGGCCAACAAGTGGTGTAAATCAAATCATCAAGAACTTCCCAAACCGAGTG

Protein sequence

MAGDNLETLWHTNMKPTANSFCKLQSIRIEMCTQLRCIFPSNIVTSLVSLDKLEIISCASLKRIFKIENQSIGDTKVVWSLRELHLLNLPNLKTVWRKNVLKFLTFPSIKRVQIRGCTKLTHVWKESIKMTTNFNTLERIDVERCEKLKYLLPSSITFLNLNELHIEKCNGMVNLFSSKVAKRLVNLSSIKVSSCKAMSCIVAAEEEIVEVIIFNKLGKLELDCLPRLTSFYSGKCMLEFISLESLVIKRCPQMETFSYGVIITPRLHSMWMEDTEFGVSWPTSGVNQIIKNFPNRV
Homology
[Close]
BLAST of Lsi10G002350 vs. ExPASy Swiss-Prot
Match: Q9LVT3 (Probable disease resistance protein At5g47260 OS=Arabidopsis thaliana OX=3702 GN=At5g47260 PE=3 SV=2)

HSP 1 Score: 49.7 bits (117), Expect = 6.7e-05
Identity = 71/275 (25.82%), Postives = 122/275 (44.36%), Query Frame = 0 

Query: 42  NIVTSLVSLDKLEIISCASLKRIFKIENQSIGDTKVVWSLRELHLLNLPNLKTVWRKNVL 101
           +++ SL++L  L +    S+      + + + D +++ SL+EL L       TV   +VL
Sbjct: 608 DVIASLLNLQVLRLFHSVSM------DLKLMEDIQLLKSLKELSL-------TVRGSSVL 667

Query: 102 KFL-----TFPSIKRVQIRGCTKLTHVWKESIKMTTNFNTLERIDVERCEKLKYLLPSSI 161
           + L        SI+R+ +   T+ T V    + +   F+  E +D+  C  L+  +    
Sbjct: 668 QRLLSIQRLASSIRRLHL---TETTIVDGGILSLNAIFSLCE-LDILGCNILEITIDWRC 727

Query: 162 T--------FLNLNELHIEKCNGMVNLFSSKVAKRLVNLSSIKVSSCKAMSCIVAAEEEI 221
           T        F N+  + I +C  + +L    +A  L  LS   VS C  M  +++ ++ +
Sbjct: 728 TIQREIIPQFQNIRTMTIHRCEYLRDLTWLLLAPCLGELS---VSECPQMEEVISKDKAM 787

Query: 222 VEV-----IIFNKLGKLELDCLPRLTSFYSGKCMLEFISLESLVIKRCPQME--TFSYGV 281
            ++       F  L KL LD LP+L S Y     L F  LE LVI+RCP++    F+   
Sbjct: 788 AKLGNTSEQPFQNLTKLVLDGLPKLESIY--WTPLPFPVLEYLVIRRCPELRRLPFNSES 847

Query: 282 IITPRLHSMWMEDTEFGVSWPTSGVNQIIKNFPNR 297
            I  ++ ++  E     V W      Q   +F NR
Sbjct: 848 TIGNQVETIIEEQVIKIVEWEDEATKQRFSHFNNR 860

[Close]
BLAST of Lsi10G002350 vs. ExPASy TrEMBL
Match: A0A1S3CHN4 (disease resistance protein At4g27190-like OS=Cucumis melo OX=3656 GN=LOC103501064 PE=4 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 2.8e-115
Identity = 210/298 (70.47%), Postives = 246/298 (82.55%), Query Frame = 0 

Query: 1    MAGDNLETLWHTNMKPTANSFCKLQSIRIEMCTQLRCIFPSNIVTSLVSLDKLEIISCAS 60
            + G NLETLWH N  PT NSFCKLQSIRIE CTQLRC+FPSN+  SL SL  ++IISCAS
Sbjct: 936  VGGYNLETLWHKNNNPTTNSFCKLQSIRIEQCTQLRCMFPSNMFASLTSLHTIQIISCAS 995

Query: 61   LKRIFKIENQSIGDTKVVWSLRELHLLNLPNLKTVWRKNVLKFLTFPSIKRVQIRGCTKL 120
            LKRIF+IENQS     VVWSL ELHLL+LPNLK VWRK+++K LTFPS+KRV+I GCTKL
Sbjct: 996  LKRIFEIENQSFSVKTVVWSLNELHLLDLPNLKHVWRKDIIKILTFPSLKRVKIHGCTKL 1055

Query: 121  THVWKESIKMTTNFNTLERIDVERCEKLKYLLPSSITFLNLNELHIEKCNGMVNLFSSKV 180
            THVWK++ K T +F++LERI+VE+C+ LKYLLPSSITFLNL ELHI+KCNGM+NLFSS V
Sbjct: 1056 THVWKDNTKETRSFDSLERIEVEKCKNLKYLLPSSITFLNLKELHIKKCNGMMNLFSSTV 1115

Query: 181  AKRLVNLSSIKVSSCKAMSCIVAA-EEEIVEVIIFNKLGKLELDCLPRLTSFYSGKCMLE 240
             K+LVNL SIKVS CK M C+V   +EE  E+I+F +L +LELD LPRL SF+SGKCMLE
Sbjct: 1116 TKKLVNLRSIKVSYCKRMRCMVEGDQEENDEIIVFKRLSRLELDYLPRLDSFHSGKCMLE 1175

Query: 241  FISLESLVIKRCPQMETFSYGVIITPRLHSMWMEDTEFGV-SWPTSGVNQIIKNFPNR 297
            F  LESLVIKRCP+M+TFSYGVII PRLHSMWM D  FGV S PT G+N+ I+NFP +
Sbjct: 1176 FPCLESLVIKRCPEMKTFSYGVIIAPRLHSMWMNDKGFGVSSSPTCGINETIQNFPKQ 1233

[Close]
BLAST of Lsi10G002350 vs. ExPASy TrEMBL
Match: A0A0A0LTN4 (NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G433370 PE=4 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 1.6e-86
Identity = 179/355 (50.42%), Postives = 226/355 (63.66%), Query Frame = 0 

Query: 11   HTNMKPTANSFCKLQSIRIEMCTQLRCIFPSNIVTSLVSLDKLEIISCASLKRIFKIE-- 70
            H ++ P    F K+++I I+ C Q+R +F  +I   L+ L ++E+I+C  ++ I  +E  
Sbjct: 824  HISLSP----FKKVKTIAIQFCGQIRNLFSFSIFKDLLDLQEIEVINCGKMEGIIFMEIG 883

Query: 71   ---------------------------------NQSI----------------------- 130
                                             +QSI                       
Sbjct: 884  DQLNICSCPLTSLQLENVDKLTSFCTKDLIQESSQSIIPFFDGQVSFPELNDLSIVGGNN 943

Query: 131  ---------GDTKVVWSLRELHLLNLPNLKTVWRKNVLKFLTFPSIKRVQIRGCTKLTHV 190
                       T V+WSL ELHLLNLPNLK VWRK+++K LTFPS+KRV+I GCTKLTHV
Sbjct: 944  LETLWHKNNNPTTVLWSLNELHLLNLPNLKQVWRKDIIKILTFPSLKRVKIHGCTKLTHV 1003

Query: 191  WKESIKMTTNFNTLERIDVERCEKLKYLLPSSITFLNLNELHIEKCNGMVNLFSSKVAKR 250
            WK++ K+T +F++LERI+VE+C+ LKYLLPSSI FLNL ELHI+KCNGM+NLFSS V K+
Sbjct: 1004 WKDNNKVTRSFDSLERIEVEKCKNLKYLLPSSIAFLNLKELHIKKCNGMINLFSSTVTKK 1063

Query: 251  LVNLSSIKVSSCKAMSCIVAAEE-EIVEVIIFNKLGKLELDCLPRLTSFYSGKCMLEFIS 298
            LVNLSSIKVS CK M C+V  ++ E  E+I F KL  LELD LPRL SFYSGKCMLEF  
Sbjct: 1064 LVNLSSIKVSYCKGMRCMVEVDQAENDEIITFKKLSTLELDYLPRLDSFYSGKCMLEFPC 1123

[Close]
BLAST of Lsi10G002350 vs. ExPASy TrEMBL
Match: A0A6J1DLB8 (disease resistance protein RPS5-like OS=Momordica charantia OX=3673 GN=LOC111022189 PE=4 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 3.1e-53
Identity = 132/297 (44.44%), Postives = 182/297 (61.28%), Query Frame = 0 

Query: 3   GDNLETLWHTNMKPTANSFCKLQSIRIEMCTQLRCIFPSNIVTSLVSLDKLEIISCASLK 62
           G +LETLWH N   T + FCKLQ+IRI+ C +L+ +FPSN+VTSLV L  LEI +C  L+
Sbjct: 639 GLSLETLWHNNGLST-HPFCKLQNIRIQYCNKLKYVFPSNVVTSLVFLKILEIYTCELLE 698

Query: 63  RIFKIENQSIGDTKVVWSLRELHLLNLPNLKTVWRKNVLKFLTFPSIKRVQIRGCTKL-- 122
            IF++E  +  + +    L +L L++LP LK VW K+  +FLTFP++++V++ GC +L  
Sbjct: 699 GIFEVEKLT-DEARATVPLTKLILISLPKLKYVWDKDPGEFLTFPNLRKVEVSGCPRLKS 758

Query: 123 --------------THVWKESIKMTTNFNTLER--------------------IDVERCE 182
                         T V  E+ K+      L +                    I V++C+
Sbjct: 759 FVPTSFIRHMDETKTLVTDEASKLVVPEMVLSKGFGDENIVTLSVETSQESNFIQVKKCD 818

Query: 183 KLKYLLPSSITFLNLNELHIEKCNGMVNLFSSKVAKRLVNLSSIKVSSCKAMSCIVAAEE 242
           K+  LLPSSI+F NLN L I +CNGM+NLFSS VAK LV+L  + +S+C+ MS I+A EE
Sbjct: 819 KMNCLLPSSISFFNLNSLIIWECNGMINLFSSSVAKSLVSLYCLTISNCEEMSSIIAREE 878

Query: 243 EIV--EVIIFNKLGKLELDCLPRLTSFYSGKCMLEFISLESLVIKRCPQMETFSYGV 262
           E    E IIF KL  LELD LPRL +FYSGK  L+F  L +L I +CP+M+ FSYG+
Sbjct: 879 EEEKDEEIIFKKLTDLELDDLPRLANFYSGKYSLKFPCLNNLRITKCPKMKPFSYGI 933

[Close]
BLAST of Lsi10G002350 vs. ExPASy TrEMBL
Match: A0A6J1JB58 (probable disease resistance protein At4g27220 OS=Cucurbita maxima OX=3661 GN=LOC111484189 PE=4 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 1.3e-51
Identity = 124/310 (40.00%), Postives = 181/310 (58.39%), Query Frame = 0 

Query: 21   FCKLQSIRIEMCTQLRCIFPSNIVTSLVSLDKLEIISCASLKRIFKIENQSIG------- 80
            F KL+SI I+ C ++R +F  +++ ++ +L ++ +  C  +  I  IE +          
Sbjct: 827  FKKLKSISIQSCGEIRNLFSISVLKAVSNLQEISVTDCGMMDEIISIETEEQSSICSITS 886

Query: 81   -----------------------------DTKVVW-SLRELHLLNLPNLKTVWRKNVLKF 140
                                         D +V +  L+ L +    NL+T+W  + L  
Sbjct: 887  LTLEKVNKLASFCTKLFIQEDPQSKIPCFDQRVSFPELKHLSITKGNNLETLWHSDGLAS 946

Query: 141  LTFPSIKRVQIRGCTKLTHVWKESIKMTTNFNTLERIDVERCEKLKYLLPSSITFLNLNE 200
             +F  ++ V+I GC +L  ++  +   +  F++LERI+V +CE L  LLPSSI FL+LNE
Sbjct: 947  NSFGKLQTVRIEGCKRLRCMFPSN--TSARFDSLERIEVRKCENLNRLLPSSILFLSLNE 1006

Query: 201  LHIEKCNGMVNLFSSKVAKRLVNLSSIKVSSCKAMSCIVAAEEEIVEVIIFNKLGKLELD 260
            L +EKCNGM++LFS+ VAK LVNL SIKV  C+ M C+VAA EE    ++F KL  +ELD
Sbjct: 1007 LSVEKCNGMMSLFSATVAKGLVNLRSIKVRCCRGMRCVVAAGEE-QGGVVFGKLKTVELD 1066

Query: 261  CLPRLTSFYSGKCMLEFISLESLVIKRCPQMETFSYGVIITPRLHSMWMEDTEFGVSWPT 294
             LP L SFY GKC +EF  LE L+I RCP+M+ FSYGV++TPRL S+ M+  EFGV+   
Sbjct: 1067 LLPELGSFYCGKCRIEFPRLECLIIGRCPEMKAFSYGVVVTPRLDSILMDGAEFGVA-AA 1126

[Close]
BLAST of Lsi10G002350 vs. ExPASy TrEMBL
Match: A0A6J1FY21 (probable disease resistance protein At4g27220 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448887 PE=4 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 4.9e-51
Identity = 124/310 (40.00%), Postives = 179/310 (57.74%), Query Frame = 0 

Query: 21   FCKLQSIRIEMCTQLRCIFPSNIVTSLVSLDKLEIISCASLKRIFKIENQSIG------- 80
            F KL+SI I+ C ++R +F  +++  + +L ++ +  C  +  I  IE +          
Sbjct: 827  FKKLKSISIQSCGEIRNLFSISVLKVVSNLQEISVTDCGMMDEIISIETEEQSSICSITS 886

Query: 81   -----------------------------DTKVVW-SLRELHLLNLPNLKTVWRKNVLKF 140
                                         D +V +  L+ L +    NL+T+W  + L  
Sbjct: 887  LTLEKVDKLTSFCTKLFIQEDPQNTIPCFDQRVSFPELKHLSITKGNNLETLWHSDGLAS 946

Query: 141  LTFPSIKRVQIRGCTKLTHVWKESIKMTTNFNTLERIDVERCEKLKYLLPSSITFLNLNE 200
             +F  ++ V+I GC +L  ++  +   +  FN+LERI+V +C  LK LLPSS+ FL+LNE
Sbjct: 947  NSFSKLQTVRIEGCKRLRCMFPSN--TSARFNSLERIEVRKCGNLKQLLPSSMLFLSLNE 1006

Query: 201  LHIEKCNGMVNLFSSKVAKRLVNLSSIKVSSCKAMSCIVAAEEEIVEVIIFNKLGKLELD 260
            L +EKCNGM++LFS+ VAK LVNL SIKV  C+ M C+VAA EE    ++F KL  +ELD
Sbjct: 1007 LSVEKCNGMMSLFSATVAKGLVNLRSIKVRCCREMRCVVAAGEE-QGGVVFGKLKSVELD 1066

Query: 261  CLPRLTSFYSGKCMLEFISLESLVIKRCPQMETFSYGVIITPRLHSMWMEDTEFGVSWPT 294
             LP L SFY GKC +EF  LE L+I RCP M+ FSYGV++TPRL S+ M+  EFGV+   
Sbjct: 1067 LLPELGSFYCGKCRIEFPRLECLIIGRCPLMKAFSYGVVVTPRLDSILMDGAEFGVA-AA 1126

[Close]
BLAST of Lsi10G002350 vs. NCBI nr
Match: XP_038902150.1 (disease resistance protein At4g27190-like [Benincasa hispida])

HSP 1 Score: 449.5 bits (1155), Expect = 2.2e-122
Identity = 218/289 (75.43%), Postives = 253/289 (87.54%), Query Frame = 0 

Query: 5    NLETLWHTNMKPTANSFCKLQSIRIEMCTQLRCIFPSNIVTSLVSLDKLEIISCASLKRI 64
            NLETLWH N +PT NSFCKLQSIRIEMCT++RC+FPSN+VTSLVSLD +EI SCASLKRI
Sbjct: 938  NLETLWHKNNEPTTNSFCKLQSIRIEMCTKIRCMFPSNMVTSLVSLDTIEITSCASLKRI 997

Query: 65   FKIENQSIGDTKVVWSLRELHLLNLPNLKTVWRKNVLKFLTFPSIKRVQIRGCTKLTHVW 124
            F+IE +S GDTKV WSLRELHLLNLPNLK VWRK+V++F+ FPS+KRV+I GCTKL H+W
Sbjct: 998  FEIEKESFGDTKVAWSLRELHLLNLPNLKHVWRKDVIEFMKFPSLKRVKIHGCTKLAHIW 1057

Query: 125  KESIKMTTNFNTLERIDVERCEKLKYLLPSSITFLNLNELHIEKCNGMVNLFSSKVAKRL 184
            KE+ KMTT+F++LE I+VERCE LKYLLPSSITFLNLNELH+++CNGM+NLFSS VAK L
Sbjct: 1058 KENTKMTTSFDSLESIEVERCENLKYLLPSSITFLNLNELHVKECNGMMNLFSSTVAKNL 1117

Query: 185  VNLSSIKVSSCKAMSCIVAAEEEIVEVIIFNKLGKLELDCLPRLTSFYSGKCMLEFISLE 244
            VNL SI+VS C+ MSCIV A+EE  E +IF KLGKLE DCLPRL SFYSGKCMLEF SLE
Sbjct: 1118 VNLRSIEVSYCEGMSCIVEAKEENDESVIFKKLGKLEFDCLPRLDSFYSGKCMLEFPSLE 1177

Query: 245  SLVIKRCPQMETFSYGVIITPRLHSMWMEDTEFGVSWPTSGVNQIIKNF 294
            SLVIKRCP+M+TFS GVI+TP L SMW+EDTEF VS  T+ VN+ I+NF
Sbjct: 1178 SLVIKRCPKMKTFSLGVIMTPELQSMWVEDTEFEVSSTTNSVNRTIQNF 1226

[Close]
BLAST of Lsi10G002350 vs. NCBI nr
Match: XP_011650062.1 (probable disease resistance protein At5g63020 [Cucumis sativus] >KAE8652346.1 hypothetical protein Csa_022345 [Cucumis sativus])

HSP 1 Score: 437.6 bits (1124), Expect = 8.7e-119
Identity = 212/298 (71.14%), Postives = 249/298 (83.56%), Query Frame = 0 

Query: 1    MAGDNLETLWHTNMKPTANSFCKLQSIRIEMCTQLRCIFPSNIVTSLVSLDKLEIISCAS 60
            + G+NLETLWH N  PT  SFCKLQSIRIE CTQLRC+FPSN++TSL SL  ++IISCAS
Sbjct: 935  VGGNNLETLWHKNNNPTTGSFCKLQSIRIEQCTQLRCMFPSNMLTSLASLHTIQIISCAS 994

Query: 61   LKRIFKIENQSIGDTKVVWSLRELHLLNLPNLKTVWRKNVLKFLTFPSIKRVQIRGCTKL 120
            LKRIF+IENQS  DT V+WSL ELHLLNLPNLK VWRK+++K LTFPS+KRV+I GCTKL
Sbjct: 995  LKRIFEIENQSFNDTTVLWSLNELHLLNLPNLKQVWRKDIIKILTFPSLKRVKIHGCTKL 1054

Query: 121  THVWKESIKMTTNFNTLERIDVERCEKLKYLLPSSITFLNLNELHIEKCNGMVNLFSSKV 180
            THVWK++ K+T +F++LERI+VE+C+ LKYLLPSSI FLNL ELHI+KCNGM+NLFSS V
Sbjct: 1055 THVWKDNNKVTRSFDSLERIEVEKCKNLKYLLPSSIAFLNLKELHIKKCNGMINLFSSTV 1114

Query: 181  AKRLVNLSSIKVSSCKAMSCIVAAEE-EIVEVIIFNKLGKLELDCLPRLTSFYSGKCMLE 240
             K+LVNLSSIKVS CK M C+V  ++ E  E+I F KL  LELD LPRL SFYSGKCMLE
Sbjct: 1115 TKKLVNLSSIKVSYCKGMRCMVEVDQAENDEIITFKKLSTLELDYLPRLDSFYSGKCMLE 1174

Query: 241  FISLESLVIKRCPQMETFSYGVIITPRLHSMWMEDTEFGVSWPTSGVNQIIKNFPNRV 298
            F  LESLVIKRCP+M+TFSYGVII PRL ++WM D EFGVS P  G+N+ I+NFP RV
Sbjct: 1175 FPCLESLVIKRCPEMKTFSYGVIIAPRLQTLWMNDKEFGVSSPACGINETIQNFPRRV 1232

[Close]
BLAST of Lsi10G002350 vs. NCBI nr
Match: XP_008462777.1 (PREDICTED: disease resistance protein At4g27190-like [Cucumis melo])

HSP 1 Score: 424.9 bits (1091), Expect = 5.9e-115
Identity = 210/298 (70.47%), Postives = 246/298 (82.55%), Query Frame = 0 

Query: 1    MAGDNLETLWHTNMKPTANSFCKLQSIRIEMCTQLRCIFPSNIVTSLVSLDKLEIISCAS 60
            + G NLETLWH N  PT NSFCKLQSIRIE CTQLRC+FPSN+  SL SL  ++IISCAS
Sbjct: 936  VGGYNLETLWHKNNNPTTNSFCKLQSIRIEQCTQLRCMFPSNMFASLTSLHTIQIISCAS 995

Query: 61   LKRIFKIENQSIGDTKVVWSLRELHLLNLPNLKTVWRKNVLKFLTFPSIKRVQIRGCTKL 120
            LKRIF+IENQS     VVWSL ELHLL+LPNLK VWRK+++K LTFPS+KRV+I GCTKL
Sbjct: 996  LKRIFEIENQSFSVKTVVWSLNELHLLDLPNLKHVWRKDIIKILTFPSLKRVKIHGCTKL 1055

Query: 121  THVWKESIKMTTNFNTLERIDVERCEKLKYLLPSSITFLNLNELHIEKCNGMVNLFSSKV 180
            THVWK++ K T +F++LERI+VE+C+ LKYLLPSSITFLNL ELHI+KCNGM+NLFSS V
Sbjct: 1056 THVWKDNTKETRSFDSLERIEVEKCKNLKYLLPSSITFLNLKELHIKKCNGMMNLFSSTV 1115

Query: 181  AKRLVNLSSIKVSSCKAMSCIVAA-EEEIVEVIIFNKLGKLELDCLPRLTSFYSGKCMLE 240
             K+LVNL SIKVS CK M C+V   +EE  E+I+F +L +LELD LPRL SF+SGKCMLE
Sbjct: 1116 TKKLVNLRSIKVSYCKRMRCMVEGDQEENDEIIVFKRLSRLELDYLPRLDSFHSGKCMLE 1175

Query: 241  FISLESLVIKRCPQMETFSYGVIITPRLHSMWMEDTEFGV-SWPTSGVNQIIKNFPNR 297
            F  LESLVIKRCP+M+TFSYGVII PRLHSMWM D  FGV S PT G+N+ I+NFP +
Sbjct: 1176 FPCLESLVIKRCPEMKTFSYGVIIAPRLHSMWMNDKGFGVSSSPTCGINETIQNFPKQ 1233

[Close]
BLAST of Lsi10G002350 vs. NCBI nr
Match: XP_022155050.1 (disease resistance protein RPS5-like [Momordica charantia])

HSP 1 Score: 218.8 bits (556), Expect = 6.4e-53
Identity = 132/297 (44.44%), Postives = 182/297 (61.28%), Query Frame = 0 

Query: 3   GDNLETLWHTNMKPTANSFCKLQSIRIEMCTQLRCIFPSNIVTSLVSLDKLEIISCASLK 62
           G +LETLWH N   T + FCKLQ+IRI+ C +L+ +FPSN+VTSLV L  LEI +C  L+
Sbjct: 639 GLSLETLWHNNGLST-HPFCKLQNIRIQYCNKLKYVFPSNVVTSLVFLKILEIYTCELLE 698

Query: 63  RIFKIENQSIGDTKVVWSLRELHLLNLPNLKTVWRKNVLKFLTFPSIKRVQIRGCTKL-- 122
            IF++E  +  + +    L +L L++LP LK VW K+  +FLTFP++++V++ GC +L  
Sbjct: 699 GIFEVEKLT-DEARATVPLTKLILISLPKLKYVWDKDPGEFLTFPNLRKVEVSGCPRLKS 758

Query: 123 --------------THVWKESIKMTTNFNTLER--------------------IDVERCE 182
                         T V  E+ K+      L +                    I V++C+
Sbjct: 759 FVPTSFIRHMDETKTLVTDEASKLVVPEMVLSKGFGDENIVTLSVETSQESNFIQVKKCD 818

Query: 183 KLKYLLPSSITFLNLNELHIEKCNGMVNLFSSKVAKRLVNLSSIKVSSCKAMSCIVAAEE 242
           K+  LLPSSI+F NLN L I +CNGM+NLFSS VAK LV+L  + +S+C+ MS I+A EE
Sbjct: 819 KMNCLLPSSISFFNLNSLIIWECNGMINLFSSSVAKSLVSLYCLTISNCEEMSSIIAREE 878

Query: 243 EIV--EVIIFNKLGKLELDCLPRLTSFYSGKCMLEFISLESLVIKRCPQMETFSYGV 262
           E    E IIF KL  LELD LPRL +FYSGK  L+F  L +L I +CP+M+ FSYG+
Sbjct: 879 EEEKDEEIIFKKLTDLELDDLPRLANFYSGKYSLKFPCLNNLRITKCPKMKPFSYGI 933

[Close]
BLAST of Lsi10G002350 vs. NCBI nr
Match: KAG7011007.1 (Disease resistance protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 218.8 bits (556), Expect = 6.4e-53
Identity = 129/321 (40.19%), Postives = 186/321 (57.94%), Query Frame = 0 

Query: 13   NMKPTAN---SFCKLQSIRIEMCTQLRCIFPSNIVTSLVSLDKLEIISCASLKRIFKIEN 72
            N+ PT +    F KL+SI I+ C ++R +F  +++ ++ +L ++ +  C  +  I  IE 
Sbjct: 766  NLSPTNSPILPFKKLKSISIQSCGEIRNLFSISVLKAVSNLQEISVTDCGMMDEIISIET 825

Query: 73   QSIG------------------------------------DTKVVW-SLRELHLLNLPNL 132
            +                                       D +V +  L+ L +    NL
Sbjct: 826  EEQSSICSITSLTLEKVDKFTSFCTKLFIQEDPQSTIPCFDQRVSFPELKHLSITKGNNL 885

Query: 133  KTVWRKNVLKFLTFPSIKRVQIRGCTKLTHVWKESIKMTTNFNTLERIDVERCEKLKYLL 192
            +T+W  + L   +F  ++ V+I GC +L  ++  +   +  FN+LERI+V +C  LK LL
Sbjct: 886  ETLWHSDGLASNSFSKLQTVRIEGCKRLRCMFPSN--TSARFNSLERIEVRKCGNLKQLL 945

Query: 193  PSSITFLNLNELHIEKCNGMVNLFSSKVAKRLVNLSSIKVSSCKAMSCIVAAEEEIVEVI 252
            PSS+ FL+LNEL +EKCNGM++LFS+ VAK LVNL SIKV SC+ M C+VAA EE    +
Sbjct: 946  PSSMLFLSLNELSVEKCNGMMSLFSATVAKGLVNLRSIKVRSCRGMRCVVAAGEE-QGGV 1005

Query: 253  IFNKLGKLELDCLPRLTSFYSGKCMLEFISLESLVIKRCPQMETFSYGVIITPRLHSMWM 294
            +F KL  +ELD LP L SFY GKC +EF  LE L+I RCP M+ FSYGV++TPRL S+ M
Sbjct: 1006 VFGKLKTVELDLLPELGSFYCGKCRIEFPRLECLIIGRCPLMKAFSYGVVVTPRLDSILM 1065

[Close]
BLAST of Lsi10G002350 vs. TAIR 10
Match: AT1G69550.1 (disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 54.3 bits (129), Expect = 1.9e-07
Identity = 67/263 (25.48%), Postives = 114/263 (43.35%), Query Frame = 0 

Query: 40   PSNIVTSLVSLDKLEIISCASL----KRIFKIENQSIGDTKVVWSLREL--HLLNLPNLK 99
            PS+I  +L++L +L+++ C+SL      I  + N          SL EL   + NL +LK
Sbjct: 781  PSSI-GNLINLPRLDLMGCSSLVELPSSIGNLINLEAFYFHGCSSLLELPSSIGNLISLK 840

Query: 100  TVWRKNVLKFLTFPS-------IKRVQIRGCTKLTHVWKESIKMTTNFNTLERIDVERCE 159
             ++ K +   +  PS       +K + + GC+ L     E      N   L+++D+  C 
Sbjct: 841  ILYLKRISSLVEIPSSIGNLINLKLLNLSGCSSLV----ELPSSIGNLINLKKLDLSGCS 900

Query: 160  KLKYLLPSSITFLNLNELHIEKCNGMVNLFSSKVAKRLVNLSSIKVSSCKAMSCIVAAEE 219
             L  L  S    +NL EL++ +C+ +V L SS     L+NL ++ +S C   S +V    
Sbjct: 901  SLVELPLSIGNLINLQELYLSECSSLVELPSS--IGNLINLKTLNLSEC---SSLVELPS 960

Query: 220  EIVEVIIFNKLGKLELDCLPRLTSFYSGKCMLEFISLESLVIKRCPQMETFSYGVIITPR 279
             I  +I   +L   E   L  L S          I+L+ L +  C  +      +     
Sbjct: 961  SIGNLINLQELYLSECSSLVELPSSIG-----NLINLKKLDLSGCSSLVELPLSIGNLIN 1020

Query: 280  LHSMWMEDTEFGVSWPTSGVNQI 290
            L ++ + +    V  P+S  N I
Sbjct: 1021 LKTLNLSECSSLVELPSSIGNLI 1028

[Close]
BLAST of Lsi10G002350 vs. TAIR 10
Match: AT5G47260.1 (ATP binding;GTP binding;nucleotide binding;nucleoside-triphosphatases )

HSP 1 Score: 49.7 bits (117), Expect = 4.8e-06
Identity = 71/275 (25.82%), Postives = 122/275 (44.36%), Query Frame = 0 

Query: 42  NIVTSLVSLDKLEIISCASLKRIFKIENQSIGDTKVVWSLRELHLLNLPNLKTVWRKNVL 101
           +++ SL++L  L +    S+      + + + D +++ SL+EL L       TV   +VL
Sbjct: 608 DVIASLLNLQVLRLFHSVSM------DLKLMEDIQLLKSLKELSL-------TVRGSSVL 667

Query: 102 KFL-----TFPSIKRVQIRGCTKLTHVWKESIKMTTNFNTLERIDVERCEKLKYLLPSSI 161
           + L        SI+R+ +   T+ T V    + +   F+  E +D+  C  L+  +    
Sbjct: 668 QRLLSIQRLASSIRRLHL---TETTIVDGGILSLNAIFSLCE-LDILGCNILEITIDWRC 727

Query: 162 T--------FLNLNELHIEKCNGMVNLFSSKVAKRLVNLSSIKVSSCKAMSCIVAAEEEI 221
           T        F N+  + I +C  + +L    +A  L  LS   VS C  M  +++ ++ +
Sbjct: 728 TIQREIIPQFQNIRTMTIHRCEYLRDLTWLLLAPCLGELS---VSECPQMEEVISKDKAM 787

Query: 222 VEV-----IIFNKLGKLELDCLPRLTSFYSGKCMLEFISLESLVIKRCPQME--TFSYGV 281
            ++       F  L KL LD LP+L S Y     L F  LE LVI+RCP++    F+   
Sbjct: 788 AKLGNTSEQPFQNLTKLVLDGLPKLESIY--WTPLPFPVLEYLVIRRCPELRRLPFNSES 847

Query: 282 IITPRLHSMWMEDTEFGVSWPTSGVNQIIKNFPNR 297
            I  ++ ++  E     V W      Q   +F NR
Sbjct: 848 TIGNQVETIIEEQVIKIVEWEDEATKQRFSHFNNR 860

[Close]
BLAST of Lsi10G002350 vs. TAIR 10
Match: AT2G14080.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 43.1 bits (100), Expect = 4.5e-04
Identity = 66/250 (26.40%), Postives = 106/250 (42.40%), Query Frame = 0 

Query: 1   MAGDNLETLWH----------------TNMK--PTANSFCKLQSIRIEMCTQLRCIFPSN 60
           M G  LE LW                  N+K  P  +S   L+ + +  C+ L    P +
Sbjct: 658 MWGSKLEKLWEEIQPLRNLKRMDLFSSKNLKELPDLSSATNLEVLNLNGCSSL-VELPFS 717

Query: 61  IVTSLVSLDKLEIISCASLKRIFKIENQSIGDTKVVWSLRELHLLNLPNLKTVWRKNVLK 120
           I  +   L KLE+  C+SL  +      SIG+   + ++   H  NL  L +        
Sbjct: 718 I-GNATKLLKLELSGCSSLLEL----PSSIGNAINLQTIDFSHCENLVELPS-------S 777

Query: 121 FLTFPSIKRVQIRGCTKLTHVWKESIKMTTNFNTLERIDVERCEKLKYLLPSSI-TFLNL 180
                ++K + +  C+ L    KE      N   L+++ +  C  LK  LPSSI    NL
Sbjct: 778 IGNATNLKELDLSCCSSL----KELPSSIGNCTNLKKLHLICCSSLKE-LPSSIGNCTNL 837

Query: 181 NELHIEKCNGMVNLFSSKVAKRLVNLSSIKVSSCKAMSCIVAAEEEIVEVIIFNKLGKLE 232
            ELH+  C+ ++ L SS      +NL  + ++ C+++  + +   +   + I N LG   
Sbjct: 838 KELHLTCCSSLIKLPSS--IGNAINLEKLILAGCESLVELPSFIGKATNLKILN-LG--Y 884

[Close]
BLAST of Lsi10G002350 vs. TAIR 10
Match: AT3G44480.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 42.7 bits (99), Expect = 5.8e-04
Identity = 41/188 (21.81%), Postives = 80/188 (42.55%), Query Frame = 0 

Query: 85  HLLNLPNLKTVWRKNVLKFLTFPSIKRVQIRGCTKLTHVWKESIKMTTNFNTLERIDVER 144
           +L  LPNL T             +++ +++R C+ L  +     K+T    +L+ +D+E 
Sbjct: 726 YLKELPNLSTA-----------TNLEELKLRNCSSLVELPSSIEKLT----SLQILDLEN 785

Query: 145 CEKLKYLLPSSITFLNLNELHIEKCNGMVNLFSSKVAKRLVNLSSIKVSSCKAMSCIVAA 204
           C  L+  LP+      L EL ++ C+ ++ L  S       NL  + +S C ++  + ++
Sbjct: 786 CSSLE-KLPAIENATKLRELKLQNCSSLIELPLS--IGTATNLKQLNISGCSSLVKLPSS 845

Query: 205 EEEIVEVIIFNKLGKLELDCLPRLTSFYSGKCML---------------EFISLESLVIK 258
             +I ++ +F+      L  LP         C L                  SL++L + 
Sbjct: 846 IGDITDLEVFDLSNCSSLVTLPSSIGNLQNLCKLIMRGCSKLEALPININLKSLDTLNLT 895

The following BLAST results are available for this feature:
BLAST of Lsi10G002350 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LVT36.7e-0525.82Probable disease resistance protein At5g47260 OS=Arabidopsis thaliana OX=3702 GN... [more]
BLAST of Lsi10G002350 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S3CHN42.8e-11570.47disease resistance protein At4g27190-like OS=Cucumis melo OX=3656 GN=LOC10350106... [more]
A0A0A0LTN41.6e-8650.42NB-ARC domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G433370 PE=4... [more]
A0A6J1DLB83.1e-5344.44disease resistance protein RPS5-like OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A6J1JB581.3e-5140.00probable disease resistance protein At4g27220 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1FY214.9e-5140.00probable disease resistance protein At4g27220 isoform X1 OS=Cucurbita moschata O... [more]
BLAST of Lsi10G002350 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038902150.12.2e-12275.43disease resistance protein At4g27190-like [Benincasa hispida][more]
XP_011650062.18.7e-11971.14probable disease resistance protein At5g63020 [Cucumis sativus] >KAE8652346.1 hy... [more]
XP_008462777.15.9e-11570.47PREDICTED: disease resistance protein At4g27190-like [Cucumis melo][more]
XP_022155050.16.4e-5344.44disease resistance protein RPS5-like [Momordica charantia][more]
KAG7011007.16.4e-5340.19Disease resistance protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
BLAST of Lsi10G002350 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G69550.11.9e-0725.48disease resistance protein (TIR-NBS-LRR class) [more]
AT5G47260.14.8e-0625.82ATP binding;GTP binding;nucleotide binding;nucleoside-triphosphatases [more]
AT2G14080.14.5e-0426.40Disease resistance protein (TIR-NBS-LRR class) family [more]
AT3G44480.15.8e-0421.81Disease resistance protein (TIR-NBS-LRR class) family [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 3..285
e-value: 2.3E-12
score: 47.4
NoneNo IPR availablePANTHERPTHR33463FAMILY NOT NAMEDcoord: 75..269
NoneNo IPR availablePANTHERPTHR33463:SF89SUBFAMILY NOT NAMEDcoord: 75..269
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 20..283

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G002350.1Lsi10G002350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
molecular_function GO:0043531 ADP binding