Lsi10G002160 (gene) Bottle gourd (USVL1VR-Ls) v1

BLAST of Lsi10G002160 vs. ExPASy Swiss-Prot
Match: Q9XIQ7 (Probable folate-biopterin transporter 7 OS=Arabidopsis thaliana OX=3702 GN=At1g64890 PE=2 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 1.4e-147
Identity = 271/429 (63.17%), Postives = 342/429 (79.72%), Query Frame = 0

Query: 9   NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILS 68
           NPIR +LG G++VQGFR FPW+  NFFL + L V+PS LQLLQNSANLPMVAKP+YG++S
Sbjct: 17  NPIRWLLGFGFFVQGFRGFPWLGANFFLTEQLRVNPSVLQLLQNSANLPMVAKPIYGVVS 76

Query: 69  DAVYISGQHRIPYIAIGAFLQAVSWIPVALMSPSRISVFTISVYLLLSNLGASIAEVAND 128
           D+VY  GQHRIPYIA+GA LQA+SW+ +A +S S +S+  +S+YLLLSNLGAS+ EVAND
Sbjct: 77  DSVYFFGQHRIPYIAVGALLQAISWLAIAFLSRSNVSILALSIYLLLSNLGASLVEVAND 136

Query: 129 AIVAEAGKQPTSSKNSQGSSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQEMFLFFG 188
           AIVAE+GKQ TS      + SGEL +FVW+ SS GGILGNLLGGIAI  FS Q  FL FG
Sbjct: 137 AIVAESGKQKTSE-----TQSGELPSFVWMVSSLGGILGNLLGGIAIKTFSAQSTFLVFG 196

Query: 189 LLLLLQFFLTIIVRERSLNLPRSSTNA-GINKQLSELSAVLKRPEIAYSIIWFAASYSII 248
           +L LLQF +TI +RE+SLNLP + + A GI K LS+LS VL++PEI+YSI W A S +++
Sbjct: 197 ILALLQFLVTINIREKSLNLPENPSPAGGIRKHLSDLSHVLRKPEISYSIAWIAVSTAVV 256

Query: 249 PALTGTMFFYQTEHLKIESSVLGISKVLGQAALLLWGVIYNRHLKSVPSRKLISSIQVLM 308
           P LTGTMFFYQT+ LKI++S+LGISKV GQ A+LLWG  YNR LK++  RKL+++IQV +
Sbjct: 257 PVLTGTMFFYQTKFLKIDASLLGISKVFGQIAMLLWGFAYNRWLKAMRPRKLLTAIQVTI 316

Query: 309 AVFMISDVLFVKGVYREMGMPDFIYVVLFSGLSEVLLFFKMLPFSVLIAQLCPPGCEGSL 368
           A F+ISD+LFVKGVYR++G+ D +YV+ FSG  E L +FK+LPF+VL+A+LCPPGCEGSL
Sbjct: 317 AFFVISDLLFVKGVYRDLGVSDSVYVLFFSGFLETLFYFKILPFTVLMARLCPPGCEGSL 376

Query: 369 MAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLAHGLIIQAVCTLVPLSCSWCIPDD 428
           MAFVMSAIALAFIVSG LG+ LAS+VGVT ++FSG   GL I+A C  +PL  +  I D+
Sbjct: 377 MAFVMSAIALAFIVSGYLGIVLASFVGVTEDDFSGFTRGLAIEACCVGIPLILTSWIYDE 436

Query: 429 IKAKGRRKE 437
            + K + K+
Sbjct: 437 AETKEKSKK 440

BLAST of Lsi10G002160 vs. ExPASy Swiss-Prot
Match: F4I5Q2 (Probable folate-biopterin transporter 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g04570 PE=2 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 4.8e-92
Identity = 191/438 (43.61%), Postives = 284/438 (64.84%), Query Frame = 0

Query: 11  IRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILSDA 70
           I  + GLGYWVQG RCFPW+A+NF +   L + PSTLQL+Q S +LPMVAKPLYG+LSD 
Sbjct: 87  ISVLCGLGYWVQGSRCFPWLALNFHMVHSLALQPSTLQLVQYSCSLPMVAKPLYGVLSDV 146

Query: 71  VYISGQHRIPYIAIGAFLQAVSWIPVALMSPSRISVFTISVYLLLSNLGASIAEVANDAI 130
           +YI    R+PYIAIG FLQ ++W  + +   +R  + ++   +LLSNLGASI EVA DA+
Sbjct: 147 LYIGSGRRVPYIAIGVFLQVLAWGSMGIFQGAREVLPSLVACVLLSNLGASITEVAKDAL 206

Query: 131 VAEAGKQPTSSKNSQGSSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQEMFLFFGLL 190
           VAE G +              LQ++  +AS+AGG+LGNLLGG  +    P+  FL F  L
Sbjct: 207 VAEYGLR---------YRINGLQSYALMASAAGGVLGNLLGGYLLLTTPPKISFLVFSAL 266

Query: 191 LLLQFFLTIIVRERSLNLPR----SSTNAGINKQLSELSAVLKRPEIAYSIIWFAASYSI 250
           L LQ  +++  +E S  LPR    SS    + KQ+S L   ++  EI+  +IW   S ++
Sbjct: 267 LSLQLVVSLSSKEESFGLPRIAETSSVLESVKKQISNLKEAIQADEISQPLIWAVVSIAM 326

Query: 251 IPALTGTMFFYQTEHLKIESSVLGISKVLGQAALLLWGVIYNRHLKSVPSRKLISSIQVL 310
           +P L+G++F YQT+ L ++ SV+G+SKV+GQ  LL   V+Y+R+LK++P R LI  IQ+L
Sbjct: 327 VPLLSGSVFCYQTQVLNLDPSVIGMSKVIGQLMLLCLTVVYDRYLKTLPMRPLIHIIQLL 386

Query: 311 MAVFMISDVLFVKGVYREMGMPDFIYVVLFSGLSEVLLFFKMLPFSVLIAQLCPPGCEGS 370
             + ++ D + VK +    G+ + +YV+ FS L+E+L  FK+LPF+V +A +CP GCEGS
Sbjct: 387 YGLSILLDYILVKQINLGFGISNEVYVLCFSSLAEILAQFKILPFAVRLASMCPQGCEGS 446

Query: 371 LMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLAHGLIIQAVCTLVPLSCSWCIPD 430
           + +F+ S + L+ IVS  LGV LA+ +G+T++ +S L+ G++IQ++  L PL     +P 
Sbjct: 447 VTSFLASTLCLSQIVSAFLGVGLANLIGITSSNYSNLSSGILIQSLAALAPLCFMHLVPM 506

Query: 431 D---IKAKGRRKESKKQK 442
               I+ +G+R  SK+ +
Sbjct: 507 SEPVIEKEGKRGISKRSR 515

BLAST of Lsi10G002160 vs. ExPASy Swiss-Prot
Match: O22780 (Probable folate-biopterin transporter 9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g33280 PE=3 SV=2)

HSP 1 Score: 335.9 bits (860), Expect = 7.0e-91
Identity = 185/436 (42.43%), Postives = 284/436 (65.14%), Query Frame = 0

Query: 17  LGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILSDAVYISGQ 76
           LGYWVQG RCF W+A+NF +   LN+ PSTLQL+Q +A+LPMVAKPLYG+LSD +YI G 
Sbjct: 90  LGYWVQGLRCFSWLALNFHMAHCLNLKPSTLQLVQYTASLPMVAKPLYGVLSDVLYIGGA 149

Query: 77  HRIPYIAIGAFLQAVSWIPVALMSPSRISVFTISVYLLLSNLGASIAEVANDAIVAEAGK 136
            R+PYI++G  LQ ++W  +A+   +R  + ++  ++LLSNLGASI EV+ DA+VAE G 
Sbjct: 150 RRVPYISVGVLLQGLAWGSLAIFPGAREVLPSLMAFILLSNLGASITEVSQDALVAEYGL 209

Query: 137 QPTSSKNSQGSSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQEMFLFFGLLLLLQFF 196
           +              LQ++  +AS+ GGILGNLLGG  + +  P+ +FL F  LL LQ  
Sbjct: 210 R---------YQINGLQSYALMASAVGGILGNLLGGYCLLKTPPRILFLAFTALLSLQLI 269

Query: 197 LTIIVRERSLNLPR--------SSTNAGINKQLSELSAVLKRPEIAYSIIWFAASYSIIP 256
           +++  +E S+NLPR        SS    + KQ  +L  +++  EI+  +IW  +S +++P
Sbjct: 270 VSLSSKEESVNLPRIGEVTPEISSVLGIVKKQFLDLKGIVQVDEISQPLIWIVSSIALVP 329

Query: 257 ALTGTMFFYQTEHLKIESSVLGISKVLGQAALLLWGVIYNRHLKSVPSRKLISSIQVLMA 316
            L+G++F YQT+ L ++ SV+G+SKV+GQ  LL   V+Y+R+ K +P R LI  +Q+L A
Sbjct: 330 LLSGSVFCYQTQVLNLDPSVIGMSKVIGQLMLLCLTVVYDRYWKKLPMRALIHIVQLLYA 389

Query: 317 VFMISDVLFVKGVYREMGMPDFIYVVLFSGLSEVLLFFKMLPFSVLIAQLCPPGCEGSLM 376
             ++ D + VK +    G+ +  +V+ FS ++E+L  FK+LPFSVL+A +CP GCEGS+ 
Sbjct: 390 FSLLFDYILVKQINLAFGISNTAFVLCFSSVAEILAQFKILPFSVLLANMCPGGCEGSIT 449

Query: 377 AFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLAHGLIIQAVCTLVPLSCSWCIP--- 436
           +F+ S + L+ +VSG  GV +A+ +G+T+  +S L  G++IQ++  LVPL     +P   
Sbjct: 450 SFLASTLCLSSVVSGFTGVGMANMIGITSKNYSNLPAGILIQSLAALVPLWFIHYVPMLE 509

Query: 437 DDIKAKGRRKESKKQK 442
              + +G+R  SKK +
Sbjct: 510 PGFEREGKRAMSKKSR 516

BLAST of Lsi10G002160 vs. ExPASy Swiss-Prot
Match: Q8RWQ5 (Probable folate-biopterin transporter 4 OS=Arabidopsis thaliana OX=3702 GN=At5g54860 PE=2 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 3.6e-55
Identity = 147/467 (31.48%), Postives = 230/467 (49.25%), Query Frame = 0

Query: 19  YWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILSDAVYISGQHR 78
           Y+ QGFR F W AV++ LKD L + PS  Q + + A  P   KPLYGI+SD + I G+ R
Sbjct: 24  YFTQGFRSFVWTAVSYQLKDRLQLSPSASQFVFSVAFFPWSIKPLYGIISDCIPIGGKKR 83

Query: 79  IPYIAIGAFLQAVSWIPVALMSPSRISVFTISVYLLLSNLGASIAEVANDAIVAEAGKQP 138
            PY+ I   L  V W+ + L S SR S   + ++L + NLG+++A+V  DA++AEA    
Sbjct: 84  TPYLVISTVLSLVPWLVLGLDSTSRSSSLYLMIFLTVQNLGSAMADVVIDAMIAEA---- 143

Query: 139 TSSKNSQGSSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQEMFLFFGLLLLLQFFLT 198
              +  + S +G+LQ+  W A + GG+ G+LLGG A+     + +FL F +L  LQ    
Sbjct: 144 --VRLEKASFAGDLQSVSWFAMAVGGVCGSLLGGYALSNLKIETIFLLFTVLPALQLLSC 203

Query: 199 IIVRERSLN---LP-------------------------------------------RSS 258
            +V E   N   LP                                           +S 
Sbjct: 204 ALVEESPANNEPLPELLDSNEFEEKSLTSNDNYPDTSKSNTRRRKGQKKGKKGDSNGKSE 263

Query: 259 TNAGINKQLSE------------LSAVLKRPEIAYSIIWFAASYSIIPALTGTMFFYQTE 318
           T    +K L+             L    K+P I   + WF  ++  +P L+  MF+YQTE
Sbjct: 264 TQKKQSKSLASQWFQSLKAATFGLGRAFKQPIILRPMAWFFIAHITVPNLSTVMFYYQTE 323

Query: 319 HLKIESSVLGISKVLGQAALLLWGVIYNRHLKSVPSRKLISSIQVLMAVFMISDVLFVKG 378
            L+++++ LG ++V+G   L+    IYNR+L+ +  RK +    + ++V ++ D++ V  
Sbjct: 324 VLQLDAAFLGTARVVGWLGLMFGTFIYNRYLQDMTLRKSLLFAHIGLSVTILLDMVLVSR 383

Query: 379 VYREMGMPDFIYVVLFSGLSEVLLFFKMLPFSVLIAQLCPPGCEGSLMAFVMSAIALAFI 428
                G+ D   V+  S L + +   K +PF +L  +LCPPG EG+L A  MS   L   
Sbjct: 384 ANVGYGVSDKTMVLFGSALGDAINQLKFMPFLILSGRLCPPGIEGTLFALFMSINNLGNT 443

BLAST of Lsi10G002160 vs. ExPASy Swiss-Prot
Match: Q9SKZ5 (Folate-biopterin transporter 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g32040 PE=1 SV=2)

HSP 1 Score: 160.2 bits (404), Expect = 5.2e-38
Identity = 119/430 (27.67%), Postives = 210/430 (48.84%), Query Frame = 0

Query: 9   NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILS 68
           +P    + + Y+VQG      +AV+F+LKD L++DP+   ++   ++LP + KPLYG +S
Sbjct: 124 SPDNVAVAMVYFVQGVLGLARLAVSFYLKDDLHLDPAETAVITGLSSLPWLVKPLYGFIS 183

Query: 69  DAVYISGQHRIPYIAIGAFLQAVSWIPVALMSPSRISVFTISVYLLLSNLGASIAEVAND 128
           D+V + G  R  Y+ +   L A SW   +LM+    S ++ +  +LL +L  + ++V  D
Sbjct: 184 DSVPLFGYRRRSYLVLSGLLGAFSW---SLMAGFVDSKYSAAFCILLGSLSVAFSDVVVD 243

Query: 129 AIVAEAGKQPTSSKNSQGSSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQEMFLFFG 188
           ++V E       ++    S SG LQ+  W +S+ GGI+ +   G  ++ +  + +F    
Sbjct: 244 SMVVE------RARGESQSVSGSLQSLCWGSSAFGGIVSSYFSGSLVESYGVRFVFGVTA 303

Query: 189 LLLLLQFFLTIIVRERSLNLPRSSTNAGI-----------NKQLSELSAVLKRPEIAYSI 248
           LL L+   + ++V E+ +  P S     I            + + +L   +K+P +    
Sbjct: 304 LLPLITSAVAVLVNEQRVVRPASGQKENITLLSPGFLQTSKQNMIQLWGAIKQPNVFLPT 363

Query: 249 IWFAASYSIIPALTGTMFFYQTEHLKIESSVLGISKVLGQAALLLWGVIYNRHLKSVPSR 308
           + F   +   P     MF++ T  L      LG  K++   A LL   +YN  LK+VP R
Sbjct: 364 L-FIFLWQATPHSDSAMFYFTTNKLGFTPEFLGRVKLVTSIASLLGVGLYNGFLKTVPLR 423

Query: 309 KLISSIQVLMAVFMISDVLFVKGVYREMGMPDFIYVVLFSGLSEVLLFFKMLPFSVLIAQ 368
           K+     +      ++ V+ V G  R++G+ D  + +  S +  VL     +P  VL A+
Sbjct: 424 KIFLVTTIFGTGLGMTQVILVSGFNRQLGISDEWFAIGDSLILTVLAQASFMPVLVLAAR 483

Query: 369 LCPPGCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLAHGLIIQAVCTLVP 428
           LCP G E +L A +MS      ++ G +G  L    G+T + F  L+  +I+  + +L+P
Sbjct: 484 LCPEGMEATLFATLMSISNGGSVLGGLMGAGLTQAFGITRDSFGNLSTLIILCNLSSLLP 543

BLAST of Lsi10G002160 vs. ExPASy TrEMBL
Match: A0A5A7VD92 (Putative folate-biopterin transporter 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602G001470 PE=3 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 2.1e-223
Identity = 408/439 (92.94%), Postives = 424/439 (96.58%), Query Frame = 0

Query: 2   MAASKVDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 61
           MA S  DNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MAKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 62  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWIPVALMSPSRISVFTISVYLLLSNLGAS 121
           PLYGILSDAVYISGQHRIPYIAIGAFLQAVSW+P+ALM PSRISVFTIS+YLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120

Query: 122 IAEVANDAIVAEAGKQPTSSKNSQGSSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQ 181
           IAEVANDAIVAEAGKQPTSSKNS+ SSSGELQAFVWVASSAGGILGNLLGG+AIDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180

Query: 182 EMFLFFGLLLLLQFFLTIIVRERSLNLPRSSTNAGINKQLSELSAVLKRPEIAYSIIWFA 241
            MFLFFGLLL LQFFLTIIVRERSLNLP+SSTNAGINKQLSEL+AVLKRPEIAYSI+W A
Sbjct: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSTNAGINKQLSELAAVLKRPEIAYSIMWLA 240

Query: 242 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVLGQAALLLWGVIYNRHLKSVPSRKLIS 301
            SYSI PALTGTMFFYQTEHLKIESSVLGISKV GQAALLLWGVIYNRHLKS+PSRKLI+
Sbjct: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIA 300

Query: 302 SIQVLMAVFMISDVLFVKGVYREMGMPDFIYVVLFSGLSEVLLFFKMLPFSVLIAQLCPP 361
           SIQV MAVFMISD+LFVKGVYREMG+PDFIYVVLFSGLSEVLLFFK+LPFSVLIA LCPP
Sbjct: 301 SIQVSMAVFMISDMLFVKGVYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360

Query: 362 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLAHGLIIQAVCTLVPLSCS 421
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTAN+FSGLA GLIIQAVCTL+PLSCS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLSCS 420

Query: 422 WCIPDDIKAKGRRKESKKQ 441
           WCIPDDIKAK RRK+SKKQ
Sbjct: 421 WCIPDDIKAKDRRKDSKKQ 439

BLAST of Lsi10G002160 vs. ExPASy TrEMBL
Match: A0A1S3CHR5 (probable folate-biopterin transporter 7 OS=Cucumis melo OX=3656 GN=LOC103501083 PE=3 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 2.1e-223
Identity = 408/439 (92.94%), Postives = 424/439 (96.58%), Query Frame = 0

Query: 2   MAASKVDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 61
           MA S  DNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MAKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 62  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWIPVALMSPSRISVFTISVYLLLSNLGAS 121
           PLYGILSDAVYISGQHRIPYIAIGAFLQAVSW+P+ALM PSRISVFTIS+YLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120

Query: 122 IAEVANDAIVAEAGKQPTSSKNSQGSSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQ 181
           IAEVANDAIVAEAGKQPTSSKNS+ SSSGELQAFVWVASSAGGILGNLLGG+AIDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180

Query: 182 EMFLFFGLLLLLQFFLTIIVRERSLNLPRSSTNAGINKQLSELSAVLKRPEIAYSIIWFA 241
            MFLFFGLLL LQFFLTIIVRERSLNLP+SSTNAGINKQLSEL+AVLKRPEIAYSI+W A
Sbjct: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSTNAGINKQLSELAAVLKRPEIAYSIMWLA 240

Query: 242 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVLGQAALLLWGVIYNRHLKSVPSRKLIS 301
            SYSI PALTGTMFFYQTEHLKIESSVLGISKV GQAALLLWGVIYNRHLKS+PSRKLI+
Sbjct: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIA 300

Query: 302 SIQVLMAVFMISDVLFVKGVYREMGMPDFIYVVLFSGLSEVLLFFKMLPFSVLIAQLCPP 361
           SIQV MAVFMISD+LFVKGVYREMG+PDFIYVVLFSGLSEVLLFFK+LPFSVLIA LCPP
Sbjct: 301 SIQVSMAVFMISDMLFVKGVYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360

Query: 362 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLAHGLIIQAVCTLVPLSCS 421
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTAN+FSGLA GLIIQAVCTL+PLSCS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLSCS 420

Query: 422 WCIPDDIKAKGRRKESKKQ 441
           WCIPDDIKAK RRK+SKKQ
Sbjct: 421 WCIPDDIKAKDRRKDSKKQ 439

BLAST of Lsi10G002160 vs. ExPASy TrEMBL
Match: A0A0A0LTK8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G431200 PE=3 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 3.6e-223
Identity = 408/439 (92.94%), Postives = 423/439 (96.36%), Query Frame = 0

Query: 2   MAASKVDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 61
           M+ S  DNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MSKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 62  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWIPVALMSPSRISVFTISVYLLLSNLGAS 121
           PLYGILSDAVYISGQHRIPYIAIGAFLQAVSW+P+ALM PSRISVFTIS+YLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120

Query: 122 IAEVANDAIVAEAGKQPTSSKNSQGSSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQ 181
           IAEVANDAIVAEAGKQPTSSKNS+ SSSGELQAFVWVASSAGGILGNLLGG+AIDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180

Query: 182 EMFLFFGLLLLLQFFLTIIVRERSLNLPRSSTNAGINKQLSELSAVLKRPEIAYSIIWFA 241
            MFLFFGLLL LQFFLTIIVRERSLNLP+SS NAGINKQLSEL+AVLKRPEIAYSIIW A
Sbjct: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSMNAGINKQLSELAAVLKRPEIAYSIIWLA 240

Query: 242 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVLGQAALLLWGVIYNRHLKSVPSRKLIS 301
            SYSI PALTGTMFFYQTEHLKIESSVLGISKV GQAALLLWGVIYNRHLKS+PSRKLIS
Sbjct: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIS 300

Query: 302 SIQVLMAVFMISDVLFVKGVYREMGMPDFIYVVLFSGLSEVLLFFKMLPFSVLIAQLCPP 361
           SIQVLMAVFMISD+LFVKGVYREMG+PDFIYVVLFSGLSEVLLFFK+LPFSVLIA LCPP
Sbjct: 301 SIQVLMAVFMISDMLFVKGVYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360

Query: 362 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLAHGLIIQAVCTLVPLSCS 421
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTAN+FSGLA GLIIQAVCTL+PL CS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCS 420

Query: 422 WCIPDDIKAKGRRKESKKQ 441
           WCIPDDIKAK RRK+SKKQ
Sbjct: 421 WCIPDDIKAKDRRKDSKKQ 439

BLAST of Lsi10G002160 vs. ExPASy TrEMBL
Match: A0A6J1FV96 (probable folate-biopterin transporter 7 OS=Cucurbita moschata OX=3662 GN=LOC111448420 PE=3 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 2.3e-222
Identity = 405/440 (92.05%), Postives = 424/440 (96.36%), Query Frame = 0

Query: 2   MAASKVDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 61
           M+ S  +NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 62  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWIPVALMSPSRISVFTISVYLLLSNLGAS 121
           PLYGILSDAVYISGQHRIPYIAIGA LQAVSWIP+A MSPS ISVFTIS+YLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120

Query: 122 IAEVANDAIVAEAGKQPTSSKNSQGSSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQ 181
           IAEVANDAIVAEAGKQPTSSKNS+ SSSGELQAFVWVASSAGGILGNLLGGI IDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180

Query: 182 EMFLFFGLLLLLQFFLTIIVRERSLNLPRSSTNAGINKQLSELSAVLKRPEIAYSIIWFA 241
           EMFLFFGLLLLLQF LTIIVRERSLNLP+SST+AGI KQLS+L  VLKRPEI YSI WFA
Sbjct: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240

Query: 242 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVLGQAALLLWGVIYNRHLKSVPSRKLIS 301
           ASYSIIPALTGTMFFYQTEHLKIESSVLGISKV+GQAALLLWGVIYNRHLKS+PSRKLIS
Sbjct: 241 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300

Query: 302 SIQVLMAVFMISDVLFVKGVYREMGMPDFIYVVLFSGLSEVLLFFKMLPFSVLIAQLCPP 361
           SIQVLMAVFMISD+LFVKGVYREMGMPDF+YVVLFSGLSEVLLFFK+LPFSVLIAQLCPP
Sbjct: 301 SIQVLMAVFMISDMLFVKGVYREMGMPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360

Query: 362 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLAHGLIIQAVCTLVPLSCS 421
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGL +GLIIQAVCT++PLSCS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS 420

Query: 422 WCIPDDIKAKGRRKESKKQK 442
           WCIPDD+KAKGR K+SKKQ+
Sbjct: 421 WCIPDDVKAKGRDKDSKKQQ 440

BLAST of Lsi10G002160 vs. ExPASy TrEMBL
Match: A0A6J1JEB7 (probable folate-biopterin transporter 7 OS=Cucurbita maxima OX=3661 GN=LOC111484245 PE=3 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 6.7e-222
Identity = 405/440 (92.05%), Postives = 424/440 (96.36%), Query Frame = 0

Query: 2   MAASKVDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 61
           M+ S  +NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 62  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWIPVALMSPSRISVFTISVYLLLSNLGAS 121
           PLYGILSDAVYISGQHRIPYIAIGA LQAVSWIP+A MSPS ISVFTIS+YLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120

Query: 122 IAEVANDAIVAEAGKQPTSSKNSQGSSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQ 181
           IAEVANDAIVAEAGKQPTSSKNS+ SSSGELQAFVWVASSAGGILGNLLGGI IDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180

Query: 182 EMFLFFGLLLLLQFFLTIIVRERSLNLPRSSTNAGINKQLSELSAVLKRPEIAYSIIWFA 241
           EMFLFFGLLLLLQF LTIIVRERSLNLP+SST+AGI KQLS+L  VLKRPEI YSI WFA
Sbjct: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240

Query: 242 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVLGQAALLLWGVIYNRHLKSVPSRKLIS 301
           ASYSIIPALTGTMFFYQTE+LKIESSVLGISKV+GQAALLLWGVIYNRHLKS+PSRKLIS
Sbjct: 241 ASYSIIPALTGTMFFYQTENLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300

Query: 302 SIQVLMAVFMISDVLFVKGVYREMGMPDFIYVVLFSGLSEVLLFFKMLPFSVLIAQLCPP 361
           SIQVLMAVFMISD+LFVKGVYREMGMPDF+YVVLFSGLSEVLLFFK+LPFSVLIAQLCPP
Sbjct: 301 SIQVLMAVFMISDMLFVKGVYREMGMPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360

Query: 362 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLAHGLIIQAVCTLVPLSCS 421
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGL +GLIIQAVCTL+PLSCS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTLLPLSCS 420

Query: 422 WCIPDDIKAKGRRKESKKQK 442
           WCIPDD+KAKGR K+SKKQ+
Sbjct: 421 WCIPDDVKAKGRDKDSKKQQ 440

BLAST of Lsi10G002160 vs. NCBI nr
Match: XP_038900302.1 (probable folate-biopterin transporter 7 [Benincasa hispida] >XP_038900303.1 probable folate-biopterin transporter 7 [Benincasa hispida] >XP_038900304.1 probable folate-biopterin transporter 7 [Benincasa hispida])

HSP 1 Score: 792.7 bits (2046), Expect = 1.6e-225
Identity = 413/440 (93.86%), Postives = 428/440 (97.27%), Query Frame = 0

Query: 2   MAASKVDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 61
           MA S  DNPI+KVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MATSNSDNPIQKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 62  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWIPVALMSPSRISVFTISVYLLLSNLGAS 121
           PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWIP+ALM PSRISVFTIS+YLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWIPIALMPPSRISVFTISIYLLLSNLGAS 120

Query: 122 IAEVANDAIVAEAGKQPTSSKNSQGSSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQ 181
           IAEVANDAIVAEAGK+PTSSKNS+ SSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKRPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQ 180

Query: 182 EMFLFFGLLLLLQFFLTIIVRERSLNLPRSSTNAGINKQLSELSAVLKRPEIAYSIIWFA 241
            MFL FGLLL LQFFLTIIVRE SLNLPRSSTNAGINKQLSEL+AVLKRP+IAYSI+WFA
Sbjct: 181 AMFLSFGLLLSLQFFLTIIVRENSLNLPRSSTNAGINKQLSELAAVLKRPDIAYSILWFA 240

Query: 242 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVLGQAALLLWGVIYNRHLKSVPSRKLIS 301
           ASYSIIPALTGTMFFYQTEHLKIESSVLGISKV GQAALLLWGVIYNRHLKSVPSRKLIS
Sbjct: 241 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSVPSRKLIS 300

Query: 302 SIQVLMAVFMISDVLFVKGVYREMGMPDFIYVVLFSGLSEVLLFFKMLPFSVLIAQLCPP 361
           SIQV MAVFMISDVLFVKGVYREMG+PDFIYVVLFSGLSEVLLFFK+LPFSVLIAQLCPP
Sbjct: 301 SIQVSMAVFMISDVLFVKGVYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360

Query: 362 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLAHGLIIQAVCTLVPLSCS 421
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVG+TAN+FSGLAHGLIIQAVCTL+PLSCS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGITANDFSGLAHGLIIQAVCTLLPLSCS 420

Query: 422 WCIPDDIKAKGRRKESKKQK 442
           WCIPDDIKAK RRK+SKKQ+
Sbjct: 421 WCIPDDIKAKDRRKDSKKQQ 440

BLAST of Lsi10G002160 vs. NCBI nr
Match: XP_008462793.1 (PREDICTED: probable folate-biopterin transporter 7 [Cucumis melo] >KAA0066312.1 putative folate-biopterin transporter 7 [Cucumis melo var. makuwa] >TYK00965.1 putative folate-biopterin transporter 7 [Cucumis melo var. makuwa])

HSP 1 Score: 784.6 bits (2025), Expect = 4.3e-223
Identity = 408/439 (92.94%), Postives = 424/439 (96.58%), Query Frame = 0

Query: 2   MAASKVDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 61
           MA S  DNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MAKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 62  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWIPVALMSPSRISVFTISVYLLLSNLGAS 121
           PLYGILSDAVYISGQHRIPYIAIGAFLQAVSW+P+ALM PSRISVFTIS+YLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120

Query: 122 IAEVANDAIVAEAGKQPTSSKNSQGSSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQ 181
           IAEVANDAIVAEAGKQPTSSKNS+ SSSGELQAFVWVASSAGGILGNLLGG+AIDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180

Query: 182 EMFLFFGLLLLLQFFLTIIVRERSLNLPRSSTNAGINKQLSELSAVLKRPEIAYSIIWFA 241
            MFLFFGLLL LQFFLTIIVRERSLNLP+SSTNAGINKQLSEL+AVLKRPEIAYSI+W A
Sbjct: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSTNAGINKQLSELAAVLKRPEIAYSIMWLA 240

Query: 242 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVLGQAALLLWGVIYNRHLKSVPSRKLIS 301
            SYSI PALTGTMFFYQTEHLKIESSVLGISKV GQAALLLWGVIYNRHLKS+PSRKLI+
Sbjct: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIA 300

Query: 302 SIQVLMAVFMISDVLFVKGVYREMGMPDFIYVVLFSGLSEVLLFFKMLPFSVLIAQLCPP 361
           SIQV MAVFMISD+LFVKGVYREMG+PDFIYVVLFSGLSEVLLFFK+LPFSVLIA LCPP
Sbjct: 301 SIQVSMAVFMISDMLFVKGVYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360

Query: 362 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLAHGLIIQAVCTLVPLSCS 421
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTAN+FSGLA GLIIQAVCTL+PLSCS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLSCS 420

Query: 422 WCIPDDIKAKGRRKESKKQ 441
           WCIPDDIKAK RRK+SKKQ
Sbjct: 421 WCIPDDIKAKDRRKDSKKQ 439

BLAST of Lsi10G002160 vs. NCBI nr
Match: XP_004151911.1 (probable folate-biopterin transporter 7 [Cucumis sativus] >XP_011650057.1 probable folate-biopterin transporter 7 [Cucumis sativus] >KGN63351.1 hypothetical protein Csa_022392 [Cucumis sativus])

HSP 1 Score: 783.9 bits (2023), Expect = 7.4e-223
Identity = 408/439 (92.94%), Postives = 423/439 (96.36%), Query Frame = 0

Query: 2   MAASKVDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 61
           M+ S  DNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MSKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 62  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWIPVALMSPSRISVFTISVYLLLSNLGAS 121
           PLYGILSDAVYISGQHRIPYIAIGAFLQAVSW+P+ALM PSRISVFTIS+YLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120

Query: 122 IAEVANDAIVAEAGKQPTSSKNSQGSSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQ 181
           IAEVANDAIVAEAGKQPTSSKNS+ SSSGELQAFVWVASSAGGILGNLLGG+AIDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180

Query: 182 EMFLFFGLLLLLQFFLTIIVRERSLNLPRSSTNAGINKQLSELSAVLKRPEIAYSIIWFA 241
            MFLFFGLLL LQFFLTIIVRERSLNLP+SS NAGINKQLSEL+AVLKRPEIAYSIIW A
Sbjct: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSMNAGINKQLSELAAVLKRPEIAYSIIWLA 240

Query: 242 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVLGQAALLLWGVIYNRHLKSVPSRKLIS 301
            SYSI PALTGTMFFYQTEHLKIESSVLGISKV GQAALLLWGVIYNRHLKS+PSRKLIS
Sbjct: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIS 300

Query: 302 SIQVLMAVFMISDVLFVKGVYREMGMPDFIYVVLFSGLSEVLLFFKMLPFSVLIAQLCPP 361
           SIQVLMAVFMISD+LFVKGVYREMG+PDFIYVVLFSGLSEVLLFFK+LPFSVLIA LCPP
Sbjct: 301 SIQVLMAVFMISDMLFVKGVYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360

Query: 362 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLAHGLIIQAVCTLVPLSCS 421
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTAN+FSGLA GLIIQAVCTL+PL CS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCS 420

Query: 422 WCIPDDIKAKGRRKESKKQ 441
           WCIPDDIKAK RRK+SKKQ
Sbjct: 421 WCIPDDIKAKDRRKDSKKQ 439

BLAST of Lsi10G002160 vs. NCBI nr
Match: XP_022943764.1 (probable folate-biopterin transporter 7 [Cucurbita moschata] >XP_022943765.1 probable folate-biopterin transporter 7 [Cucurbita moschata])

HSP 1 Score: 781.2 bits (2016), Expect = 4.8e-222
Identity = 405/440 (92.05%), Postives = 424/440 (96.36%), Query Frame = 0

Query: 2   MAASKVDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 61
           M+ S  +NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 62  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWIPVALMSPSRISVFTISVYLLLSNLGAS 121
           PLYGILSDAVYISGQHRIPYIAIGA LQAVSWIP+A MSPS ISVFTIS+YLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120

Query: 122 IAEVANDAIVAEAGKQPTSSKNSQGSSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQ 181
           IAEVANDAIVAEAGKQPTSSKNS+ SSSGELQAFVWVASSAGGILGNLLGGI IDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180

Query: 182 EMFLFFGLLLLLQFFLTIIVRERSLNLPRSSTNAGINKQLSELSAVLKRPEIAYSIIWFA 241
           EMFLFFGLLLLLQF LTIIVRERSLNLP+SST+AGI KQLS+L  VLKRPEI YSI WFA
Sbjct: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240

Query: 242 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVLGQAALLLWGVIYNRHLKSVPSRKLIS 301
           ASYSIIPALTGTMFFYQTEHLKIESSVLGISKV+GQAALLLWGVIYNRHLKS+PSRKLIS
Sbjct: 241 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300

Query: 302 SIQVLMAVFMISDVLFVKGVYREMGMPDFIYVVLFSGLSEVLLFFKMLPFSVLIAQLCPP 361
           SIQVLMAVFMISD+LFVKGVYREMGMPDF+YVVLFSGLSEVLLFFK+LPFSVLIAQLCPP
Sbjct: 301 SIQVLMAVFMISDMLFVKGVYREMGMPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360

Query: 362 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLAHGLIIQAVCTLVPLSCS 421
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGL +GLIIQAVCT++PLSCS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS 420

Query: 422 WCIPDDIKAKGRRKESKKQK 442
           WCIPDD+KAKGR K+SKKQ+
Sbjct: 421 WCIPDDVKAKGRDKDSKKQQ 440

BLAST of Lsi10G002160 vs. NCBI nr
Match: XP_022986535.1 (probable folate-biopterin transporter 7 [Cucurbita maxima] >XP_022986536.1 probable folate-biopterin transporter 7 [Cucurbita maxima])

HSP 1 Score: 779.6 bits (2012), Expect = 1.4e-221
Identity = 405/440 (92.05%), Postives = 424/440 (96.36%), Query Frame = 0

Query: 2   MAASKVDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 61
           M+ S  +NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 62  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWIPVALMSPSRISVFTISVYLLLSNLGAS 121
           PLYGILSDAVYISGQHRIPYIAIGA LQAVSWIP+A MSPS ISVFTIS+YLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120

Query: 122 IAEVANDAIVAEAGKQPTSSKNSQGSSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQ 181
           IAEVANDAIVAEAGKQPTSSKNS+ SSSGELQAFVWVASSAGGILGNLLGGI IDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180

Query: 182 EMFLFFGLLLLLQFFLTIIVRERSLNLPRSSTNAGINKQLSELSAVLKRPEIAYSIIWFA 241
           EMFLFFGLLLLLQF LTIIVRERSLNLP+SST+AGI KQLS+L  VLKRPEI YSI WFA
Sbjct: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240

Query: 242 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVLGQAALLLWGVIYNRHLKSVPSRKLIS 301
           ASYSIIPALTGTMFFYQTE+LKIESSVLGISKV+GQAALLLWGVIYNRHLKS+PSRKLIS
Sbjct: 241 ASYSIIPALTGTMFFYQTENLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300

Query: 302 SIQVLMAVFMISDVLFVKGVYREMGMPDFIYVVLFSGLSEVLLFFKMLPFSVLIAQLCPP 361
           SIQVLMAVFMISD+LFVKGVYREMGMPDF+YVVLFSGLSEVLLFFK+LPFSVLIAQLCPP
Sbjct: 301 SIQVLMAVFMISDMLFVKGVYREMGMPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360

Query: 362 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLAHGLIIQAVCTLVPLSCS 421
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGL +GLIIQAVCTL+PLSCS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTLLPLSCS 420

Query: 422 WCIPDDIKAKGRRKESKKQK 442
           WCIPDD+KAKGR K+SKKQ+
Sbjct: 421 WCIPDDVKAKGRDKDSKKQQ 440

BLAST of Lsi10G002160 vs. TAIR 10
Match: AT1G64890.1 (Major facilitator superfamily protein )

HSP 1 Score: 524.2 bits (1349), Expect = 9.8e-149
Identity = 271/429 (63.17%), Postives = 342/429 (79.72%), Query Frame = 0

Query: 9   NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILS 68
           NPIR +LG G++VQGFR FPW+  NFFL + L V+PS LQLLQNSANLPMVAKP+YG++S
Sbjct: 17  NPIRWLLGFGFFVQGFRGFPWLGANFFLTEQLRVNPSVLQLLQNSANLPMVAKPIYGVVS 76

Query: 69  DAVYISGQHRIPYIAIGAFLQAVSWIPVALMSPSRISVFTISVYLLLSNLGASIAEVAND 128
           D+VY  GQHRIPYIA+GA LQA+SW+ +A +S S +S+  +S+YLLLSNLGAS+ EVAND
Sbjct: 77  DSVYFFGQHRIPYIAVGALLQAISWLAIAFLSRSNVSILALSIYLLLSNLGASLVEVAND 136

Query: 129 AIVAEAGKQPTSSKNSQGSSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQEMFLFFG 188
           AIVAE+GKQ TS      + SGEL +FVW+ SS GGILGNLLGGIAI  FS Q  FL FG
Sbjct: 137 AIVAESGKQKTSE-----TQSGELPSFVWMVSSLGGILGNLLGGIAIKTFSAQSTFLVFG 196

Query: 189 LLLLLQFFLTIIVRERSLNLPRSSTNA-GINKQLSELSAVLKRPEIAYSIIWFAASYSII 248
           +L LLQF +TI +RE+SLNLP + + A GI K LS+LS VL++PEI+YSI W A S +++
Sbjct: 197 ILALLQFLVTINIREKSLNLPENPSPAGGIRKHLSDLSHVLRKPEISYSIAWIAVSTAVV 256

Query: 249 PALTGTMFFYQTEHLKIESSVLGISKVLGQAALLLWGVIYNRHLKSVPSRKLISSIQVLM 308
           P LTGTMFFYQT+ LKI++S+LGISKV GQ A+LLWG  YNR LK++  RKL+++IQV +
Sbjct: 257 PVLTGTMFFYQTKFLKIDASLLGISKVFGQIAMLLWGFAYNRWLKAMRPRKLLTAIQVTI 316

Query: 309 AVFMISDVLFVKGVYREMGMPDFIYVVLFSGLSEVLLFFKMLPFSVLIAQLCPPGCEGSL 368
           A F+ISD+LFVKGVYR++G+ D +YV+ FSG  E L +FK+LPF+VL+A+LCPPGCEGSL
Sbjct: 317 AFFVISDLLFVKGVYRDLGVSDSVYVLFFSGFLETLFYFKILPFTVLMARLCPPGCEGSL 376

Query: 369 MAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLAHGLIIQAVCTLVPLSCSWCIPDD 428
           MAFVMSAIALAFIVSG LG+ LAS+VGVT ++FSG   GL I+A C  +PL  +  I D+
Sbjct: 377 MAFVMSAIALAFIVSGYLGIVLASFVGVTEDDFSGFTRGLAIEACCVGIPLILTSWIYDE 436

Query: 429 IKAKGRRKE 437
            + K + K+
Sbjct: 437 AETKEKSKK 440

BLAST of Lsi10G002160 vs. TAIR 10
Match: AT1G04570.1 (Major facilitator superfamily protein )

HSP 1 Score: 339.7 bits (870), Expect = 3.4e-93
Identity = 191/438 (43.61%), Postives = 284/438 (64.84%), Query Frame = 0

Query: 11  IRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILSDA 70
           I  + GLGYWVQG RCFPW+A+NF +   L + PSTLQL+Q S +LPMVAKPLYG+LSD 
Sbjct: 87  ISVLCGLGYWVQGSRCFPWLALNFHMVHSLALQPSTLQLVQYSCSLPMVAKPLYGVLSDV 146

Query: 71  VYISGQHRIPYIAIGAFLQAVSWIPVALMSPSRISVFTISVYLLLSNLGASIAEVANDAI 130
           +YI    R+PYIAIG FLQ ++W  + +   +R  + ++   +LLSNLGASI EVA DA+
Sbjct: 147 LYIGSGRRVPYIAIGVFLQVLAWGSMGIFQGAREVLPSLVACVLLSNLGASITEVAKDAL 206

Query: 131 VAEAGKQPTSSKNSQGSSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQEMFLFFGLL 190
           VAE G +              LQ++  +AS+AGG+LGNLLGG  +    P+  FL F  L
Sbjct: 207 VAEYGLR---------YRINGLQSYALMASAAGGVLGNLLGGYLLLTTPPKISFLVFSAL 266

Query: 191 LLLQFFLTIIVRERSLNLPR----SSTNAGINKQLSELSAVLKRPEIAYSIIWFAASYSI 250
           L LQ  +++  +E S  LPR    SS    + KQ+S L   ++  EI+  +IW   S ++
Sbjct: 267 LSLQLVVSLSSKEESFGLPRIAETSSVLESVKKQISNLKEAIQADEISQPLIWAVVSIAM 326

Query: 251 IPALTGTMFFYQTEHLKIESSVLGISKVLGQAALLLWGVIYNRHLKSVPSRKLISSIQVL 310
           +P L+G++F YQT+ L ++ SV+G+SKV+GQ  LL   V+Y+R+LK++P R LI  IQ+L
Sbjct: 327 VPLLSGSVFCYQTQVLNLDPSVIGMSKVIGQLMLLCLTVVYDRYLKTLPMRPLIHIIQLL 386

Query: 311 MAVFMISDVLFVKGVYREMGMPDFIYVVLFSGLSEVLLFFKMLPFSVLIAQLCPPGCEGS 370
             + ++ D + VK +    G+ + +YV+ FS L+E+L  FK+LPF+V +A +CP GCEGS
Sbjct: 387 YGLSILLDYILVKQINLGFGISNEVYVLCFSSLAEILAQFKILPFAVRLASMCPQGCEGS 446

Query: 371 LMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLAHGLIIQAVCTLVPLSCSWCIPD 430
           + +F+ S + L+ IVS  LGV LA+ +G+T++ +S L+ G++IQ++  L PL     +P 
Sbjct: 447 VTSFLASTLCLSQIVSAFLGVGLANLIGITSSNYSNLSSGILIQSLAALAPLCFMHLVPM 506

Query: 431 D---IKAKGRRKESKKQK 442
               I+ +G+R  SK+ +
Sbjct: 507 SEPVIEKEGKRGISKRSR 515

BLAST of Lsi10G002160 vs. TAIR 10
Match: AT2G33280.1 (Major facilitator superfamily protein )

HSP 1 Score: 277.7 bits (709), Expect = 1.6e-74
Identity = 159/395 (40.25%), Postives = 251/395 (63.54%), Query Frame = 0

Query: 58  MVAKPLYGILSDAVYISGQHRIPYIAIGAFLQAVSWIPVALMSPSRISVFTISVYLLLSN 117
           MVAKPLYG+LSD +YI G  R+PYI++G  LQ ++W  +A+   +R  + ++  ++LLSN
Sbjct: 1   MVAKPLYGVLSDVLYIGGARRVPYISVGVLLQGLAWGSLAIFPGAREVLPSLMAFILLSN 60

Query: 118 LGASIAEVANDAIVAEAGKQPTSSKNSQGSSSGELQAFVWVASSAGGILGNLLGGIAIDR 177
           LGASI EV+ DA+VAE G +              LQ++  +AS+ GGILGNLLGG  + +
Sbjct: 61  LGASITEVSQDALVAEYGLR---------YQINGLQSYALMASAVGGILGNLLGGYCLLK 120

Query: 178 FSPQEMFLFFGLLLLLQFFLTIIVRERSLNLPR--------SSTNAGINKQLSELSAVLK 237
             P+ +FL F  LL LQ  +++  +E S+NLPR        SS    + KQ  +L  +++
Sbjct: 121 TPPRILFLAFTALLSLQLIVSLSSKEESVNLPRIGEVTPEISSVLGIVKKQFLDLKGIVQ 180

Query: 238 RPEIAYSIIWFAASYSIIPALTGTMFFYQTEHLKIESSVLGISKVLGQAALLLWGVIYNR 297
             EI+  +IW  +S +++P L+G++F YQT+ L ++ SV+G+SKV+GQ  LL   V+Y+R
Sbjct: 181 VDEISQPLIWIVSSIALVPLLSGSVFCYQTQVLNLDPSVIGMSKVIGQLMLLCLTVVYDR 240

Query: 298 HLKSVPSRKLISSIQVLMAVFMISDVLFVKGVYREMGMPDFIYVVLFSGLSEVLLFFKML 357
           + K +P R LI  +Q+L A  ++ D + VK +    G+ +  +V+ FS ++E+L  FK+L
Sbjct: 241 YWKKLPMRALIHIVQLLYAFSLLFDYILVKQINLAFGISNTAFVLCFSSVAEILAQFKIL 300

Query: 358 PFSVLIAQLCPPGCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLAHGLII 417
           PFSVL+A +CP GCEGS+ +F+ S + L+ +VSG  GV +A+ +G+T+  +S L  G++I
Sbjct: 301 PFSVLLANMCPGGCEGSITSFLASTLCLSSVVSGFTGVGMANMIGITSKNYSNLPAGILI 360

Query: 418 QAVCTLVPLSCSWCIP---DDIKAKGRRKESKKQK 442
           Q++  LVPL     +P      + +G+R  SKK +
Sbjct: 361 QSLAALVPLWFIHYVPMLEPGFEREGKRAMSKKSR 386

BLAST of Lsi10G002160 vs. TAIR 10
Match: AT5G54860.1 (Major facilitator superfamily protein )

HSP 1 Score: 217.2 bits (552), Expect = 2.6e-56
Identity = 147/467 (31.48%), Postives = 230/467 (49.25%), Query Frame = 0

Query: 19  YWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILSDAVYISGQHR 78
           Y+ QGFR F W AV++ LKD L + PS  Q + + A  P   KPLYGI+SD + I G+ R
Sbjct: 24  YFTQGFRSFVWTAVSYQLKDRLQLSPSASQFVFSVAFFPWSIKPLYGIISDCIPIGGKKR 83

Query: 79  IPYIAIGAFLQAVSWIPVALMSPSRISVFTISVYLLLSNLGASIAEVANDAIVAEAGKQP 138
            PY+ I   L  V W+ + L S SR S   + ++L + NLG+++A+V  DA++AEA    
Sbjct: 84  TPYLVISTVLSLVPWLVLGLDSTSRSSSLYLMIFLTVQNLGSAMADVVIDAMIAEA---- 143

Query: 139 TSSKNSQGSSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQEMFLFFGLLLLLQFFLT 198
              +  + S +G+LQ+  W A + GG+ G+LLGG A+     + +FL F +L  LQ    
Sbjct: 144 --VRLEKASFAGDLQSVSWFAMAVGGVCGSLLGGYALSNLKIETIFLLFTVLPALQLLSC 203

Query: 199 IIVRERSLN---LP-------------------------------------------RSS 258
            +V E   N   LP                                           +S 
Sbjct: 204 ALVEESPANNEPLPELLDSNEFEEKSLTSNDNYPDTSKSNTRRRKGQKKGKKGDSNGKSE 263

Query: 259 TNAGINKQLSE------------LSAVLKRPEIAYSIIWFAASYSIIPALTGTMFFYQTE 318
           T    +K L+             L    K+P I   + WF  ++  +P L+  MF+YQTE
Sbjct: 264 TQKKQSKSLASQWFQSLKAATFGLGRAFKQPIILRPMAWFFIAHITVPNLSTVMFYYQTE 323

Query: 319 HLKIESSVLGISKVLGQAALLLWGVIYNRHLKSVPSRKLISSIQVLMAVFMISDVLFVKG 378
            L+++++ LG ++V+G   L+    IYNR+L+ +  RK +    + ++V ++ D++ V  
Sbjct: 324 VLQLDAAFLGTARVVGWLGLMFGTFIYNRYLQDMTLRKSLLFAHIGLSVTILLDMVLVSR 383

Query: 379 VYREMGMPDFIYVVLFSGLSEVLLFFKMLPFSVLIAQLCPPGCEGSLMAFVMSAIALAFI 428
                G+ D   V+  S L + +   K +PF +L  +LCPPG EG+L A  MS   L   
Sbjct: 384 ANVGYGVSDKTMVLFGSALGDAINQLKFMPFLILSGRLCPPGIEGTLFALFMSINNLGNT 443

BLAST of Lsi10G002160 vs. TAIR 10
Match: AT2G32040.1 (Major facilitator superfamily protein )

HSP 1 Score: 160.2 bits (404), Expect = 3.7e-39
Identity = 119/430 (27.67%), Postives = 210/430 (48.84%), Query Frame = 0

Query: 9   NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILS 68
           +P    + + Y+VQG      +AV+F+LKD L++DP+   ++   ++LP + KPLYG +S
Sbjct: 124 SPDNVAVAMVYFVQGVLGLARLAVSFYLKDDLHLDPAETAVITGLSSLPWLVKPLYGFIS 183

Query: 69  DAVYISGQHRIPYIAIGAFLQAVSWIPVALMSPSRISVFTISVYLLLSNLGASIAEVAND 128
           D+V + G  R  Y+ +   L A SW   +LM+    S ++ +  +LL +L  + ++V  D
Sbjct: 184 DSVPLFGYRRRSYLVLSGLLGAFSW---SLMAGFVDSKYSAAFCILLGSLSVAFSDVVVD 243

Query: 129 AIVAEAGKQPTSSKNSQGSSSGELQAFVWVASSAGGILGNLLGGIAIDRFSPQEMFLFFG 188
           ++V E       ++    S SG LQ+  W +S+ GGI+ +   G  ++ +  + +F    
Sbjct: 244 SMVVE------RARGESQSVSGSLQSLCWGSSAFGGIVSSYFSGSLVESYGVRFVFGVTA 303

Query: 189 LLLLLQFFLTIIVRERSLNLPRSSTNAGI-----------NKQLSELSAVLKRPEIAYSI 248
           LL L+   + ++V E+ +  P S     I            + + +L   +K+P +    
Sbjct: 304 LLPLITSAVAVLVNEQRVVRPASGQKENITLLSPGFLQTSKQNMIQLWGAIKQPNVFLPT 363

Query: 249 IWFAASYSIIPALTGTMFFYQTEHLKIESSVLGISKVLGQAALLLWGVIYNRHLKSVPSR 308
           + F   +   P     MF++ T  L      LG  K++   A LL   +YN  LK+VP R
Sbjct: 364 L-FIFLWQATPHSDSAMFYFTTNKLGFTPEFLGRVKLVTSIASLLGVGLYNGFLKTVPLR 423

Query: 309 KLISSIQVLMAVFMISDVLFVKGVYREMGMPDFIYVVLFSGLSEVLLFFKMLPFSVLIAQ 368
           K+     +      ++ V+ V G  R++G+ D  + +  S +  VL     +P  VL A+
Sbjct: 424 KIFLVTTIFGTGLGMTQVILVSGFNRQLGISDEWFAIGDSLILTVLAQASFMPVLVLAAR 483

Query: 369 LCPPGCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLAHGLIIQAVCTLVP 428
           LCP G E +L A +MS      ++ G +G  L    G+T + F  L+  +I+  + +L+P
Sbjct: 484 LCPEGMEATLFATLMSISNGGSVLGGLMGAGLTQAFGITRDSFGNLSTLIILCNLSSLLP 543