Lsi10G001670 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview
NameLsi10G001670
Typegene
OrganismLagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
DescriptionNatural resistance-associated macrophage protein, putative
Locationchr10: 2689200 .. 2692617 (-)
RNA-Seq ExpressionLsi10G001670
SyntenyLsi10G001670
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCATCGCCAATTTCGAATTTCGATAACCCAAAAAAATCTCCCCATTTTCCCAATTCCCATTCCACGTCCCTTTCGCTTTATCTCCACGCTGTCTTCCCACCTCACACAGATTTTCCAGGTATCCCATCAACACGATGCCGTTTCCGGCTCGAGCTCGCTCGGTGGCCCCCCCTTTTGGCTTTTGCTCTTGTTCGGGGTTTTAAAACTTTAATCGAGGTCGCAAATCTTGGAGGACGAGAGTTTGATTCGAACCCCTTTGTTGGGTATTGTGAAATTTTTCATCTTTTCTGTGGATCAGTCAAGATTGATTTTGGTGACGAGCGAGCTGAGCTATTCTTTTCACACTTGGTGTTCTTTCTTTTTTATAATTGGGTCCCCAAATTCTATACGATCATCCTGCAAGTTTTTTATCATTCTGAAAAAGGAAAAGTGGAAATAAAAGAACGTTCAGTGCGAAGGCCACTGGCGGTTTCTTTCTTTTTCTTCCTCGCCTCGCTTCACGATAAAGCTTCTGGAAGAGGATTTTTGGGCAAGTACTCGAAACGCAGGCTAGAAAAGTCGAAGCTCAGCAATCCACTCCGTAGTTTGGATTTTTAGACACCCACTTCCGTTAATTGTCCAATCATGCATCCCGATGATCAGCAGCAACAGCCATTGATAGAGGAGGAAGATGAGGAAACTGCCTACGACTTGACGGAGAAGGTTGTGGTAATTGGGATCGACGAAGCGGAATCTGATTCGGACGTGGGTTTGCTGCCGTTTTCATGGAGGAAGCTCTGGCTGTTCACAGGGCCTGGGTTTTTGATGAGCATAGCTTTTCTAGATCCTGGGAATTTGGAGTCCAATCTTCAGGCTGGTGCTATAGCTGGATATTCTTTGTTGTGGCTTCTGTTGTGGGCTACGGCCATGGGGCTTTTGATCCAGTTGCTTTCGGCTAGGCTTGGCGTTGCCACTGGAAGGCACTTGGCCGAGCTGTGTCGAGAAGAGTATCCAACTTGGGCGAGGATTGTGCTGTGGGTCATGGCGGAGTTCGCCCTCATCGGGGCTGATATACAGGAAGTCATTGGGAGTGCTATTGCTATTAAGATTCTGAGTAATGGGGCTTTGCCTCTCTGGGCTGGAGTTATCATCACTGCTTTAGATTGGTAATTTTTTTATTCTATCCCAATTCCCAAATTGGGTTTGTTGTGTATGATTATGTGCTTCCCCAATTTGAAAGTGTTTTTGTTGATTCTTGTCAGTTTTATCTTCCTGTTTCTTGAGAATTATGGTGTGAGGAAACTGGAGGCTGTTTTTGCTGTTCTTATTGCTACAATGGCACTTTCATTTGCTTGGATGTTTGGTGAAACTAAGCCAGATGGGAAGGAGCTTCTATTGGGTGAGGACTGAGGAGACTTTTTATTTTTTTGGTCTTTCATTATGTTTTGTATGTAAGTTTTCTGCTCATTATTGTCTTTGCCACTTACACAATGGCCAACAATGGATCTTCTTTACAGGTATTTTGATTCCAAAACTTAGCTCCAAGACAATTAAACAGGCTGTTGCTGTTGTGGGGTGTATCATTATGCCTCATAATGTGTTTTTGCACTCTGCTCTTGTGCAGTCGCGAGATGTCGATCCGAGTAAGAAAGGGCGGGTCCAAGAAGCTCTGAGATACTACTCCATTGAGTCCACTCTTGCCCTTTTTGTCTCCTTCATCATCAACTTGTTTGTTACAACTGTGTTTGCTAAAGCTTTCTATGGCACAGACATAGCGAGTAGCATTGGTCTTGTAAATGCAGGCCAATATCTTCAAGATAAATATGGTGGAGGGATTTTCCCCATTCTGTACATCTGGGCTATTGGTTTATTAGCTGCTGGCCAAAGTAGCACCATCACTGGTACCTATGCAGGACAGTTTATCATGAGTGGATTCCTGAACTTGAAATTGAAAAAATGGCTAAGAGCTTTGATAACTCGAAGCTTTGCAATCGTGCCAACTATGATTGTCGCTCTCGTGTTCGAAACTTCTGACTCAATGGTCGATGTTCTGAATGAATGGCTCAATGTCCTCCAGTCAATTCAGATTCCCTTTGCTCTGATTCCTCTTCTCTGTTTGGCTTCGAAGGAACATCTAATGGGTAGTTTCAAAATTGGCCCCGTTTTAAAGGTATGTGTCTGATGATCACTATTCTACGTTTATGTTATACAGTCCATTGCAAACTAAATGATGAATCAATTACTTGGTCACAACTTTGTTTTTCTTTTGATTTGTAGATAATATCTAGTTTAGTTACGAGTACCTTGCATATTAATTTCATGTTTTGGGATCATGGATGGTAGCCTTGTTGATTTTGTTGAATCGAACCTTAAATCCAAAAATTTTAACGAGACCCCACAAGTGACTGCTAATACTGCAGAGGTTTGGACATTAGAATTCTAATTCCACTCTTAATTTTTGCTAGACTCGAACACTTAGTTGATAATTTAGGACATACACTATCAAGCAATGGAGGAAATTCTAGCTATATTATAATGGAATGCATTCCTATAGATTTAGAAGTCTTTGGCCTTCATCTTTGAATCTGTAAATGGCATTAATGGATTGAGAATCTTCAATTCTTTTTTACTCTAAGCACAAAAAAAAAAACATGCTGTTGTCTGTGGTTTAGGTCTAAGATCATCTAAAAGTCCATGTAGTTCAATAAATAGTTTGGAAACAAAACAATTGTAGTTCAAGAACTCACAACATGCCTTTCTGCATCTTCAGACGATCTCTTGGCTGGTGGCAGTCTTGGTGATGGCCATCAACGGGTATCTTTTGGTGAGCTTCTTCTCATCGGAAGTGAACGGCGTGCTCATTGCCATTTTCGTATTTGTCTTCATTGCTGCATATCTTGCCTTTGTTGTTTACCTCGTCTATCGAAGCATTTCGTTTTCAAGTTGGCACAACTTCATTAACCGCAAGACTTACACGGCCAGTGGAAACTGAGTGATCATCTTCAACTTCGACTCAGACTTATTTAGATTGCAGATCGATAATTGTCAAATAATTGCCTTGTCCTCGAGCATCTGTTAAAACGCAGTCGGAGAACACTTCCCAGGAGTAACATATCAGTTAGTTTTGAGATATGAGTTATGATTTGGCATGTTTTTCCTTGAGAAAAGCTGTTCTGGTTAACTTTGTATTATAATTGTTCATTTTTCTGTTTACGTACAGTCAAATGAAGCAGATTCAGCCTGAAATCTGGTGGGTAATTTAAGTTTATGTAGTCTTTGTAGTCTTAGGTTATATCAATGTCATTCAAGTGTTCTGAATTTGCTTGGCACAATTAATCAAATTTACTCATCACTTAGAACTGATGTACTGATTACCGACGGCAAATGATTAAGGCCACATATGGGGGATGGTTTTTTATTTATTTATTTATTTATTTA

mRNA sequence

TCCATCGCCAATTTCGAATTTCGATAACCCAAAAAAATCTCCCCATTTTCCCAATTCCCATTCCACGTCCCTTTCGCTTTATCTCCACGCTGTCTTCCCACCTCACACAGATTTTCCAGGTATCCCATCAACACGATGCCGTTTCCGGCTCGAGCTCGCTCGGTGGCCCCCCCTTTTGGCTTTTGCTCTTGTTCGGGGTTTTAAAACTTTAATCGAGGTCGCAAATCTTGGAGGACGAGAGTTTGATTCGAACCCCTTTGTTGGGTATTGTGAAATTTTTCATCTTTTCTGTGGATCAGTCAAGATTGATTTTGGTGACGAGCGAGCTGAGCTATTCTTTTCACACTTGGTGTTCTTTCTTTTTTATAATTGGGTCCCCAAATTCTATACGATCATCCTGCAAGTTTTTTATCATTCTGAAAAAGGAAAAGTGGAAATAAAAGAACGTTCAGTGCGAAGGCCACTGGCGGTTTCTTTCTTTTTCTTCCTCGCCTCGCTTCACGATAAAGCTTCTGGAAGAGGATTTTTGGGCAAGTACTCGAAACGCAGGCTAGAAAAGTCGAAGCTCAGCAATCCACTCCGTAGTTTGGATTTTTAGACACCCACTTCCGTTAATTGTCCAATCATGCATCCCGATGATCAGCAGCAACAGCCATTGATAGAGGAGGAAGATGAGGAAACTGCCTACGACTTGACGGAGAAGGTTGTGGTAATTGGGATCGACGAAGCGGAATCTGATTCGGACGTGGGTTTGCTGCCGTTTTCATGGAGGAAGCTCTGGCTGTTCACAGGGCCTGGGTTTTTGATGAGCATAGCTTTTCTAGATCCTGGGAATTTGGAGTCCAATCTTCAGGCTGGTGCTATAGCTGGATATTCTTTGTTGTGGCTTCTGTTGTGGGCTACGGCCATGGGGCTTTTGATCCAGTTGCTTTCGGCTAGGCTTGGCGTTGCCACTGGAAGGCACTTGGCCGAGCTGTGTCGAGAAGAGTATCCAACTTGGGCGAGGATTGTGCTGTGGGTCATGGCGGAGTTCGCCCTCATCGGGGCTGATATACAGGAAGTCATTGGGAGTGCTATTGCTATTAAGATTCTGAGTAATGGGGCTTTGCCTCTCTGGGCTGGAGTTATCATCACTGCTTTAGATTGTTTTATCTTCCTGTTTCTTGAGAATTATGGTGTGAGGAAACTGGAGGCTGTTTTTGCTGTTCTTATTGCTACAATGGCACTTTCATTTGCTTGGATGTTTGGTGAAACTAAGCCAGATGGGAAGGAGCTTCTATTGGGTATTTTGATTCCAAAACTTAGCTCCAAGACAATTAAACAGGCTGTTGCTGTTGTGGGGTGTATCATTATGCCTCATAATGTGTTTTTGCACTCTGCTCTTGTGCAGTCGCGAGATGTCGATCCGAGTAAGAAAGGGCGGGTCCAAGAAGCTCTGAGATACTACTCCATTGAGTCCACTCTTGCCCTTTTTGTCTCCTTCATCATCAACTTGTTTGTTACAACTGTGTTTGCTAAAGCTTTCTATGGCACAGACATAGCGAGTAGCATTGGTCTTGTAAATGCAGGCCAATATCTTCAAGATAAATATGGTGGAGGGATTTTCCCCATTCTGTACATCTGGGCTATTGGTTTATTAGCTGCTGGCCAAAGTAGCACCATCACTGGTACCTATGCAGGACAGTTTATCATGAGTGGATTCCTGAACTTGAAATTGAAAAAATGGCTAAGAGCTTTGATAACTCGAAGCTTTGCAATCGTGCCAACTATGATTGTCGCTCTCGTGTTCGAAACTTCTGACTCAATGGTCGATGTTCTGAATGAATGGCTCAATGTCCTCCAGTCAATTCAGATTCCCTTTGCTCTGATTCCTCTTCTCTGTTTGGCTTCGAAGGAACATCTAATGGGTAGTTTCAAAATTGGCCCCGTTTTAAAGACGATCTCTTGGCTGGTGGCAGTCTTGGTGATGGCCATCAACGGGTATCTTTTGGTGAGCTTCTTCTCATCGGAAGTGAACGGCGTGCTCATTGCCATTTTCGTATTTGTCTTCATTGCTGCATATCTTGCCTTTGTTGTTTACCTCGTCTATCGAAGCATTTCGTTTTCAAGTTGGCACAACTTCATTAACCGCAAGACTTACACGGCCAGTGGAAACTGAGTGATCATCTTCAACTTCGACTCAGACTTATTTAGATTGCAGATCGATAATTGTCAAATAATTGCCTTGTCCTCGAGCATCTGTTAAAACGCAGTCGGAGAACACTTCCCAGGAGTAACATATCAGTTAGTTTTGAGATATGAGTTATGATTTGGCATGTTTTTCCTTGAGAAAAGCTGTTCTGGTTAACTTTGTATTATAATTGTTCATTTTTCTGTTTACGTACAGTCAAATGAAGCAGATTCAGCCTGAAATCTGGTGGGTAATTTAAGTTTATGTAGTCTTTGTAGTCTTAGGTTATATCAATGTCATTCAAGTGTTCTGAATTTGCTTGGCACAATTAATCAAATTTACTCATCACTTAGAACTGATGTACTGATTACCGACGGCAAATGATTAAGGCCACATATGGGGGATGGTTTTTTATTTATTTATTTATTTATTTA

Coding sequence (CDS)

ATGCATCCCGATGATCAGCAGCAACAGCCATTGATAGAGGAGGAAGATGAGGAAACTGCCTACGACTTGACGGAGAAGGTTGTGGTAATTGGGATCGACGAAGCGGAATCTGATTCGGACGTGGGTTTGCTGCCGTTTTCATGGAGGAAGCTCTGGCTGTTCACAGGGCCTGGGTTTTTGATGAGCATAGCTTTTCTAGATCCTGGGAATTTGGAGTCCAATCTTCAGGCTGGTGCTATAGCTGGATATTCTTTGTTGTGGCTTCTGTTGTGGGCTACGGCCATGGGGCTTTTGATCCAGTTGCTTTCGGCTAGGCTTGGCGTTGCCACTGGAAGGCACTTGGCCGAGCTGTGTCGAGAAGAGTATCCAACTTGGGCGAGGATTGTGCTGTGGGTCATGGCGGAGTTCGCCCTCATCGGGGCTGATATACAGGAAGTCATTGGGAGTGCTATTGCTATTAAGATTCTGAGTAATGGGGCTTTGCCTCTCTGGGCTGGAGTTATCATCACTGCTTTAGATTGTTTTATCTTCCTGTTTCTTGAGAATTATGGTGTGAGGAAACTGGAGGCTGTTTTTGCTGTTCTTATTGCTACAATGGCACTTTCATTTGCTTGGATGTTTGGTGAAACTAAGCCAGATGGGAAGGAGCTTCTATTGGGTATTTTGATTCCAAAACTTAGCTCCAAGACAATTAAACAGGCTGTTGCTGTTGTGGGGTGTATCATTATGCCTCATAATGTGTTTTTGCACTCTGCTCTTGTGCAGTCGCGAGATGTCGATCCGAGTAAGAAAGGGCGGGTCCAAGAAGCTCTGAGATACTACTCCATTGAGTCCACTCTTGCCCTTTTTGTCTCCTTCATCATCAACTTGTTTGTTACAACTGTGTTTGCTAAAGCTTTCTATGGCACAGACATAGCGAGTAGCATTGGTCTTGTAAATGCAGGCCAATATCTTCAAGATAAATATGGTGGAGGGATTTTCCCCATTCTGTACATCTGGGCTATTGGTTTATTAGCTGCTGGCCAAAGTAGCACCATCACTGGTACCTATGCAGGACAGTTTATCATGAGTGGATTCCTGAACTTGAAATTGAAAAAATGGCTAAGAGCTTTGATAACTCGAAGCTTTGCAATCGTGCCAACTATGATTGTCGCTCTCGTGTTCGAAACTTCTGACTCAATGGTCGATGTTCTGAATGAATGGCTCAATGTCCTCCAGTCAATTCAGATTCCCTTTGCTCTGATTCCTCTTCTCTGTTTGGCTTCGAAGGAACATCTAATGGGTAGTTTCAAAATTGGCCCCGTTTTAAAGACGATCTCTTGGCTGGTGGCAGTCTTGGTGATGGCCATCAACGGGTATCTTTTGGTGAGCTTCTTCTCATCGGAAGTGAACGGCGTGCTCATTGCCATTTTCGTATTTGTCTTCATTGCTGCATATCTTGCCTTTGTTGTTTACCTCGTCTATCGAAGCATTTCGTTTTCAAGTTGGCACAACTTCATTAACCGCAAGACTTACACGGCCAGTGGAAACTGA

Protein sequence

MHPDDQQQQPLIEEEDEETAYDLTEKVVVIGIDEAESDSDVGLLPFSWRKLWLFTGPGFLMSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCREEYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFLENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGCIIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAFYGTDIASSIGLVNAGQYLQDKYGGGIFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFLNLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCLASKEHLMGSFKIGPVLKTISWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYLAFVVYLVYRSISFSSWHNFINRKTYTASGN
Homology
BLAST of Lsi10G001670 vs. ExPASy Swiss-Prot
Match: Q9SNV9 (Metal transporter Nramp3 OS=Arabidopsis thaliana OX=3702 GN=NRAMP3 PE=1 SV=2)

HSP 1 Score: 753.8 bits (1945), Expect = 1.2e-216
Identity = 387/501 (77.25%), Postives = 448/501 (89.42%), Query Frame = 0

Query: 3   PDDQQQQPLI--EEEDEETAYDLTEKVVVIGIDEAESDSDVGL-----LPFSWRKLWLFT 62
           P  +  +PL+  EEE+EETAYD TEKV ++  +E E D + G+      PFSW+KLWLFT
Sbjct: 2   PQLENNEPLLINEEEEEETAYDETEKVHIVR-NEEEDDLEHGVGCGGAPPFSWKKLWLFT 61

Query: 63  GPGFLMSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLA 122
           GPGFLMSIAFLDPGNLE +LQAGA+AGYSLLWLL+WATAMGLL+QLLSARLGVATGRHLA
Sbjct: 62  GPGFLMSIAFLDPGNLEGDLQAGAVAGYSLLWLLMWATAMGLLVQLLSARLGVATGRHLA 121

Query: 123 ELCREEYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCF 182
           ELCR+EYPTWAR+VLWVMAE ALIG+DIQEVIGSAIAIKILSNG LPLWAGV+ITALDCF
Sbjct: 122 ELCRDEYPTWARMVLWVMAELALIGSDIQEVIGSAIAIKILSNGILPLWAGVVITALDCF 181

Query: 183 IFLFLENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAV 242
           +FLFLENYG+RKLEAVFAVLIATM +SFAWMFG+ KP G ELL+GIL+PKLSS+TI++AV
Sbjct: 182 VFLFLENYGIRKLEAVFAVLIATMGVSFAWMFGQAKPSGSELLIGILVPKLSSRTIQKAV 241

Query: 243 AVVGCIIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTV 302
            VVGCIIMPHNVFLHSALVQSR+VD  +K RVQEAL YY+IEST+ALF+SF+INLFVTTV
Sbjct: 242 GVVGCIIMPHNVFLHSALVQSREVDKRQKYRVQEALNYYTIESTIALFISFLINLFVTTV 301

Query: 303 FAKAFYGTDIASSIGLVNAGQYLQDKYGGGIFPILYIWAIGLLAAGQSSTITGTYAGQFI 362
           FAK FY TD+A+SIGLVNAGQYLQ+KYGGG+FPILYIWAIGLLAAGQSSTITGTYAGQFI
Sbjct: 302 FAKGFYNTDLANSIGLVNAGQYLQEKYGGGVFPILYIWAIGLLAAGQSSTITGTYAGQFI 361

Query: 363 MSGFLNLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALI 422
           M GFLN K+KKWLRALITRS AI+PT+IVALVF++S++ +DVLNEWLNVLQSIQIPFALI
Sbjct: 362 MGGFLNFKMKKWLRALITRSCAIIPTIIVALVFDSSEATLDVLNEWLNVLQSIQIPFALI 421

Query: 423 PLLCLASKEHLMGSFKIGPVLKTISWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVF 482
           PLLCL SKE +MGSFKIGP+ KTI+WLVA LV+ INGYLL+ FFS+EV+G++   FV +F
Sbjct: 422 PLLCLVSKEQIMGSFKIGPLYKTIAWLVAALVIMINGYLLLEFFSNEVSGIVYTGFVTLF 481

Query: 483 IAAYLAFVVYLVYRSISFSSW 497
            A+Y AF++YL+ R I+F+ W
Sbjct: 482 TASYGAFILYLIARGITFTPW 501

BLAST of Lsi10G001670 vs. ExPASy Swiss-Prot
Match: Q9FN18 (Metal transporter Nramp4 OS=Arabidopsis thaliana OX=3702 GN=NRAMP4 PE=2 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 1.5e-214
Identity = 381/497 (76.66%), Postives = 444/497 (89.34%), Query Frame = 0

Query: 4   DDQQQQPLIEEEDEETAYDLTEKVVVIGIDE---AESDSDVGLLP-FSWRKLWLFTGPGF 63
           +  +++PL+    EE AY+ TEKV+++GIDE   A+ D D G  P FSW+KLWLFTGPGF
Sbjct: 3   ETDRERPLL--ASEERAYEETEKVLIVGIDEEEDADYDDDPGNSPKFSWKKLWLFTGPGF 62

Query: 64  LMSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCR 123
           LMSIAFLDPGNLES+LQAGAIAGYSL+WLL+WATA+GLLIQLLSARLGVATGRHLAELCR
Sbjct: 63  LMSIAFLDPGNLESDLQAGAIAGYSLIWLLMWATAIGLLIQLLSARLGVATGRHLAELCR 122

Query: 124 EEYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLF 183
           EEYPTWAR+VLW+MAE ALIGADIQEVIGSAIAIKILSNG +PLWAGV+ITALDCFIFLF
Sbjct: 123 EEYPTWARMVLWIMAEIALIGADIQEVIGSAIAIKILSNGLVPLWAGVVITALDCFIFLF 182

Query: 184 LENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVG 243
           LENYG+RKLEAVFA+LIATMAL+FAWMFG+TKP G ELL+G L+PKLSS+TIKQAV +VG
Sbjct: 183 LENYGIRKLEAVFAILIATMALAFAWMFGQTKPSGTELLVGALVPKLSSRTIKQAVGIVG 242

Query: 244 CIIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKA 303
           CIIMPHNVFLHSALVQSR+VDP K+ RV+EAL+YYSIEST AL VSFIIN+FVTTVFAK+
Sbjct: 243 CIIMPHNVFLHSALVQSREVDPKKRFRVKEALKYYSIESTGALAVSFIINVFVTTVFAKS 302

Query: 304 FYGTDIASSIGLVNAGQYLQDKYGGGIFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGF 363
           FYGT+IA +IGL NAGQYLQDKYGGG FPILYIWAIG+LAAGQSSTITGTYAGQFIM GF
Sbjct: 303 FYGTEIADTIGLANAGQYLQDKYGGGFFPILYIWAIGVLAAGQSSTITGTYAGQFIMGGF 362

Query: 364 LNLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLC 423
           LNLK+KKW+RALITRS AI+PTMIVALVF++SDSM+D LNEWLNVLQS+QIPFA+IPLLC
Sbjct: 363 LNLKMKKWVRALITRSCAIIPTMIVALVFDSSDSMLDELNEWLNVLQSVQIPFAVIPLLC 422

Query: 424 LASKEHLMGSFKIGPVLKTISWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAY 483
           L S E +MGSFKI P+++TISW+VA LV+AINGYL+V FFS     +++ + V +F  AY
Sbjct: 423 LVSNEQIMGSFKIQPLVQTISWIVAALVIAINGYLMVDFFSGAATNLILLVPVIIFAIAY 482

Query: 484 LAFVVYLVYRSISFSSW 497
           + FV+YL+ R ++++ W
Sbjct: 483 VVFVLYLISRGLTYTPW 497

BLAST of Lsi10G001670 vs. ExPASy Swiss-Prot
Match: Q9C6B2 (Metal transporter Nramp2 OS=Arabidopsis thaliana OX=3702 GN=NRAMP2 PE=2 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 2.2e-205
Identity = 371/495 (74.95%), Postives = 425/495 (85.86%), Query Frame = 0

Query: 3   PDDQQQQPLIEEEDEETAYDLTEKVVVIGIDEAESDSDVGLL--PFSWRKLWLFTGPGFL 62
           P   Q  P  + E  E A++  EK++++   E+  D   G    PFSWRKLWLFTGPGFL
Sbjct: 20  PPPSQSLPSTDSE-SEAAFETNEKILIVDF-ESPDDPTTGDTPPPFSWRKLWLFTGPGFL 79

Query: 63  MSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 122
           MSIAFLDPGNLE +LQAGAIAGYSLLWLL+WATAMGLLIQ+LSAR+GVATGRHLAELCR+
Sbjct: 80  MSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWATAMGLLIQMLSARVGVATGRHLAELCRD 139

Query: 123 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 182
           EYPTWAR VLW MAE ALIGADIQEVIGSAIAI+ILS G LPLWAGV+ITA DCF+FLFL
Sbjct: 140 EYPTWARYVLWSMAELALIGADIQEVIGSAIAIQILSRGFLPLWAGVVITASDCFLFLFL 199

Query: 183 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 242
           ENYGVRKLEAVFAVLIATM LSFAWMFGETKP GKEL++GIL+P+LSSKTI+QAV VVGC
Sbjct: 200 ENYGVRKLEAVFAVLIATMGLSFAWMFGETKPSGKELMIGILLPRLSSKTIRQAVGVVGC 259

Query: 243 IIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF 302
           +IMPHNVFLHSALVQSR +DP +K RVQEAL YY IES++ALF+SF+INLFVTTVFAK F
Sbjct: 260 VIMPHNVFLHSALVQSRKIDPKRKSRVQEALNYYLIESSVALFISFMINLFVTTVFAKGF 319

Query: 303 YGTDIASSIGLVNAGQYLQDKYGGGIFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 362
           YGT+ A++IGLVNAGQYLQ+K+GGG+ PILYIW IGLLAAGQSSTITGTYAGQFIM GFL
Sbjct: 320 YGTEKANNIGLVNAGQYLQEKFGGGLLPILYIWGIGLLAAGQSSTITGTYAGQFIMGGFL 379

Query: 363 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 422
           NL+LKKW+RA+ITRS AIVPTMIVA+VF TS++ +DVLNEWLNVLQS+QIPFAL+PLL L
Sbjct: 380 NLRLKKWMRAVITRSCAIVPTMIVAIVFNTSEASLDVLNEWLNVLQSVQIPFALLPLLTL 439

Query: 423 ASKEHLMGSFKIGPVLKTISWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL 482
            SKE +MG FKIGP+L+ I+W VA LVM INGYLL+ FF SEV+G L  + V V+  AY+
Sbjct: 440 VSKEEIMGDFKIGPILQRIAWTVAALVMIINGYLLLDFFVSEVDGFLFGVTVCVWTTAYI 499

Query: 483 AFVVYLVYRSISFSS 496
           AF+VYL+  S  F S
Sbjct: 500 AFIVYLISHSNFFPS 512

BLAST of Lsi10G001670 vs. ExPASy Swiss-Prot
Match: Q2QN30 (Metal transporter Nramp6 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP6 PE=2 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 8.4e-197
Identity = 361/495 (72.93%), Postives = 419/495 (84.65%), Query Frame = 0

Query: 10  PLIEEED---EETAYDLTEKVVVIGIDEAESDSD------------VGLLPFSWRKLWLF 69
           P  EE+D   EE AY+ TEKV+V   D  ++D D             G+ PFSWRKLWLF
Sbjct: 31  PSNEEDDDDLEERAYEATEKVIVSISDFPDADDDEEESGLATSTAASGIPPFSWRKLWLF 90

Query: 70  TGPGFLMSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHL 129
           TGPGFLMSIAFLDPGNLE +LQAGA+AG +LLWLLLWAT+MGLL+QLL+AR+GVATGRHL
Sbjct: 91  TGPGFLMSIAFLDPGNLEGDLQAGAVAGDTLLWLLLWATSMGLLVQLLAARVGVATGRHL 150

Query: 130 AELCREEYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDC 189
           AELCR+EYP+WAR  LW+MAE A++GADIQEVIGSAIAIKILS G LPLWAGV+ITALDC
Sbjct: 151 AELCRDEYPSWARRALWLMAEVAMVGADIQEVIGSAIAIKILSRGFLPLWAGVVITALDC 210

Query: 190 FIFLFLENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQA 249
           FIFL LENYGVRKLEAVFA+LIATMA+SFAWMF +TKP+ K L +GIL+PKLSS+TI+QA
Sbjct: 211 FIFLSLENYGVRKLEAVFAILIATMAVSFAWMFTDTKPNMKNLFIGILVPKLSSRTIRQA 270

Query: 250 VAVVGCIIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLALFVSFIINLFVTT 309
           V VVGC+IMPHNVFLHSALVQSR +DP+K+ +V+EALRYYSIEST+AL VSF+INLFVTT
Sbjct: 271 VGVVGCVIMPHNVFLHSALVQSRKIDPNKEHQVREALRYYSIESTIALAVSFMINLFVTT 330

Query: 310 VFAKAFYGTDIASSIGLVNAGQYLQDKYGGGIFPILYIWAIGLLAAGQSSTITGTYAGQF 369
           VFAK FYGT  A +IGL NAGQYLQ+K+GGG FPILYIW IGLLAAGQSSTITGTYAGQF
Sbjct: 331 VFAKGFYGTKEAGNIGLENAGQYLQEKFGGGFFPILYIWGIGLLAAGQSSTITGTYAGQF 390

Query: 370 IMSGFLNLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFAL 429
           IM GFLNLKLKKW+R+LITRSFAIVPT+IVAL F+ SDS+ DVLNEWLNVLQSIQIPFAL
Sbjct: 391 IMGGFLNLKLKKWIRSLITRSFAIVPTIIVALFFDKSDSL-DVLNEWLNVLQSIQIPFAL 450

Query: 430 IPLLCLASKEHLMGSFKIGPVLKTISWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFV 489
           IPL+ L SKE +MG FKIG   + ++W VA L++ INGYLL+ FFSSE+ G+L    + V
Sbjct: 451 IPLITLVSKEKVMGVFKIGRNTQAVTWTVATLLITINGYLLLDFFSSEIRGLLSGSILCV 510

BLAST of Lsi10G001670 vs. ExPASy Swiss-Prot
Match: Q10Q65 (Metal transporter Nramp2 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP2 PE=2 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 1.2e-190
Identity = 348/487 (71.46%), Postives = 398/487 (81.72%), Query Frame = 0

Query: 14  EEDEETAYDLTEKVVVIGIDEAESDSDVG----LLP-FSWRKLWLFTGPGFLMSIAFLDP 73
           +E +E AYD  +KV +   D    D   G    + P FSWRKLW FTGPGFLM IAFLDP
Sbjct: 28  DEYDERAYDSDDKVSIAVSDSDSEDGGGGGGDAMRPAFSWRKLWRFTGPGFLMCIAFLDP 87

Query: 74  GNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCREEYPTWARI 133
           GNLE +LQAGA AGY LLWLLLWAT MG L+QLLSARLGVATG+HLAELCREEYP WA  
Sbjct: 88  GNLEGDLQAGAAAGYQLLWLLLWATVMGALVQLLSARLGVATGKHLAELCREEYPPWATR 147

Query: 134 VLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFLENYGVRKL 193
            LW M E AL+GADIQEVIGSAIAIKILS G +PLW GV+ITA DCFIFLFLENYGVRKL
Sbjct: 148 ALWAMTELALVGADIQEVIGSAIAIKILSAGTVPLWGGVVITAFDCFIFLFLENYGVRKL 207

Query: 194 EAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGCIIMPHNVF 253
           EA F VLIA MA+SFA MFGETKP GKELL+G+++PKLSS+TIKQAV +VGCIIMPHNVF
Sbjct: 208 EAFFGVLIAVMAVSFAIMFGETKPSGKELLIGLVVPKLSSRTIKQAVGIVGCIIMPHNVF 267

Query: 254 LHSALVQSRDVDPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAFYGTDIASS 313
           LHSALVQSR +D +KK RVQEA+ YY+IES LAL VSF IN+ VTTVFAK FYG++ A  
Sbjct: 268 LHSALVQSRKIDTNKKSRVQEAVFYYNIESILALIVSFFINICVTTVFAKGFYGSEQADG 327

Query: 314 IGLVNAGQYLQDKYGGGIFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFLNLKLKKWL 373
           IGL NAGQYLQ KYG   FPILYIWAIGLLA+GQSSTITGTYAGQF+M GFLNL+LKKWL
Sbjct: 328 IGLENAGQYLQQKYGTAFFPILYIWAIGLLASGQSSTITGTYAGQFVMGGFLNLRLKKWL 387

Query: 374 RALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCLASKEHLMG 433
           RA+ITRSFAI+PTMIVAL F+T D  +D+LNE LNVLQSIQIPFALIPL+ L SKE +MG
Sbjct: 388 RAMITRSFAIIPTMIVALFFDTEDPTMDILNEALNVLQSIQIPFALIPLITLVSKEQVMG 447

Query: 434 SFKIGPVLKTISWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYLAFVVYLVY 493
           SF +GP+ K ISW+V V +M INGYL++SF+++EV G L+   + V +A YLAF+VYL+ 
Sbjct: 448 SFVVGPITKVISWIVTVFLMLINGYLILSFYATEVRGALVRSSLCVVLAVYLAFIVYLIM 507

Query: 494 RSISFSS 496
           R+ S  S
Sbjct: 508 RNTSLYS 514

BLAST of Lsi10G001670 vs. ExPASy TrEMBL
Match: A0A5A7SSI5 (Metal transporter Nramp3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold465G00300 PE=3 SV=1)

HSP 1 Score: 946.4 bits (2445), Expect = 4.8e-272
Identity = 494/510 (96.86%), Postives = 505/510 (99.02%), Query Frame = 0

Query: 1   MHPDDQQQQPLIEEEDEETAYDLTEKVVVIGIDEAESDSDVGLLPFSWRKLWLFTGPGFL 60
           MHPDDQQQQPLIEEE+EETAYDLTEKVVVIGIDE ESDSDVGLLPFSWRKLWLFTGPGFL
Sbjct: 1   MHPDDQQQQPLIEEEEEETAYDLTEKVVVIGIDETESDSDVGLLPFSWRKLWLFTGPGFL 60

Query: 61  MSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120
           MSIAFLDPGNLESNLQAGA AGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE
Sbjct: 61  MSIAFLDPGNLESNLQAGATAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120

Query: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180
           EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL
Sbjct: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180

Query: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240
           ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC
Sbjct: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240

Query: 241 IIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF 300
           IIMPHNVFLHSALVQSRD++PSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF
Sbjct: 241 IIMPHNVFLHSALVQSRDINPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF 300

Query: 301 YGTDIASSIGLVNAGQYLQDKYGGGIFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360
           YGTDIA+SIGLVNAG+YL+DKYGGG+FPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL
Sbjct: 301 YGTDIANSIGLVNAGKYLEDKYGGGLFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360

Query: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420
           NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL
Sbjct: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420

Query: 421 ASKEHLMGSFKIGPVLKTISWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL 480
           ASKEHLMG+F+IGPVLKT SWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL
Sbjct: 421 ASKEHLMGTFRIGPVLKTTSWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL 480

Query: 481 AFVVYLVYRSISFSSWHNFINRKTYTASGN 511
           AFVVYLVYRSISF+SWHNFINRKTY  +GN
Sbjct: 481 AFVVYLVYRSISFTSWHNFINRKTYLGNGN 510

BLAST of Lsi10G001670 vs. ExPASy TrEMBL
Match: A0A0A0LN03 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423700 PE=3 SV=1)

HSP 1 Score: 944.9 bits (2441), Expect = 1.4e-271
Identity = 493/510 (96.67%), Postives = 503/510 (98.63%), Query Frame = 0

Query: 1   MHPDDQQQQPLIEEEDEETAYDLTEKVVVIGIDEAESDSDVGLLPFSWRKLWLFTGPGFL 60
           MHPDDQQQQPLIEEE+EETAYDLTEKVVVIGIDE ESDSDVGLLPFSWRKLWLFTGPGFL
Sbjct: 1   MHPDDQQQQPLIEEEEEETAYDLTEKVVVIGIDETESDSDVGLLPFSWRKLWLFTGPGFL 60

Query: 61  MSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120
           MSIAFLDPGNLESNLQAGA AGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE
Sbjct: 61  MSIAFLDPGNLESNLQAGATAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120

Query: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180
           EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGV ITALDCFIFLFL
Sbjct: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVTITALDCFIFLFL 180

Query: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240
           ENYGVRKLEAVFAVLIATMALSFAWMFG+TKPDGKELLLGILIPKLSSKTIKQAVAVVGC
Sbjct: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGDTKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240

Query: 241 IIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF 300
           IIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLAL VSFIINLFVTTVFAKAF
Sbjct: 241 IIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLALLVSFIINLFVTTVFAKAF 300

Query: 301 YGTDIASSIGLVNAGQYLQDKYGGGIFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360
           YGTDIA+SIGLVNAGQYLQDKYGGG+FPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL
Sbjct: 301 YGTDIANSIGLVNAGQYLQDKYGGGLFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360

Query: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420
           NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL
Sbjct: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420

Query: 421 ASKEHLMGSFKIGPVLKTISWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL 480
           ASKEHLMG+F+IGPVLKT SWLVAVLVMAINGYLLVSFFSSEVNGV++AIFVFVFIAAYL
Sbjct: 421 ASKEHLMGTFRIGPVLKTTSWLVAVLVMAINGYLLVSFFSSEVNGVVVAIFVFVFIAAYL 480

Query: 481 AFVVYLVYRSISFSSWHNFINRKTYTASGN 511
           AFVVYLVYRSISF+SWHNFINRKTY  +GN
Sbjct: 481 AFVVYLVYRSISFTSWHNFINRKTYVGNGN 510

BLAST of Lsi10G001670 vs. ExPASy TrEMBL
Match: A0A5D3BH32 (Metal transporter Nramp3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold232G00750 PE=3 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 1.8e-271
Identity = 493/510 (96.67%), Postives = 505/510 (99.02%), Query Frame = 0

Query: 1   MHPDDQQQQPLIEEEDEETAYDLTEKVVVIGIDEAESDSDVGLLPFSWRKLWLFTGPGFL 60
           MHPDDQQQQPLIEEE+EETAYDLTEKVVVIGIDE ESDSDVGLLPFSWRKLWLFTGPGFL
Sbjct: 1   MHPDDQQQQPLIEEEEEETAYDLTEKVVVIGIDETESDSDVGLLPFSWRKLWLFTGPGFL 60

Query: 61  MSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120
           MSIAFLDPGNLESNLQAGA AGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE
Sbjct: 61  MSIAFLDPGNLESNLQAGATAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120

Query: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180
           EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL
Sbjct: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180

Query: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240
           ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC
Sbjct: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240

Query: 241 IIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF 300
           IIMPHNVFLHSALVQSRD++PSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF
Sbjct: 241 IIMPHNVFLHSALVQSRDINPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF 300

Query: 301 YGTDIASSIGLVNAGQYLQDKYGGGIFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360
           YGT+IA+SIGLVNAG+YL+DKYGGG+FPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL
Sbjct: 301 YGTNIANSIGLVNAGKYLEDKYGGGLFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360

Query: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420
           NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL
Sbjct: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420

Query: 421 ASKEHLMGSFKIGPVLKTISWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL 480
           ASKEHLMG+F+IGPVLKT SWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL
Sbjct: 421 ASKEHLMGTFRIGPVLKTTSWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL 480

Query: 481 AFVVYLVYRSISFSSWHNFINRKTYTASGN 511
           AFVVYLVYRSISF+SWHNFINRKTY  +GN
Sbjct: 481 AFVVYLVYRSISFTSWHNFINRKTYLGNGN 510

BLAST of Lsi10G001670 vs. ExPASy TrEMBL
Match: A0A1S3C1X3 (metal transporter Nramp3-like OS=Cucumis melo OX=3656 GN=LOC103495920 PE=3 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 1.8e-271
Identity = 493/510 (96.67%), Postives = 505/510 (99.02%), Query Frame = 0

Query: 1   MHPDDQQQQPLIEEEDEETAYDLTEKVVVIGIDEAESDSDVGLLPFSWRKLWLFTGPGFL 60
           MHPDDQQQQPLIEEE+EETAYDLTEKVVVIGIDE ESDSDVGLLPFSWRKLWLFTGPGFL
Sbjct: 1   MHPDDQQQQPLIEEEEEETAYDLTEKVVVIGIDETESDSDVGLLPFSWRKLWLFTGPGFL 60

Query: 61  MSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120
           MSIAFLDPGNLESNLQAGA AGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE
Sbjct: 61  MSIAFLDPGNLESNLQAGATAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120

Query: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180
           EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL
Sbjct: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180

Query: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240
           ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC
Sbjct: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240

Query: 241 IIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF 300
           IIMPHNVFLHSALVQSRD++PSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF
Sbjct: 241 IIMPHNVFLHSALVQSRDINPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF 300

Query: 301 YGTDIASSIGLVNAGQYLQDKYGGGIFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360
           YGT+IA+SIGLVNAG+YL+DKYGGG+FPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL
Sbjct: 301 YGTNIANSIGLVNAGKYLEDKYGGGLFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360

Query: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420
           NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL
Sbjct: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420

Query: 421 ASKEHLMGSFKIGPVLKTISWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL 480
           ASKEHLMG+F+IGPVLKT SWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL
Sbjct: 421 ASKEHLMGTFRIGPVLKTTSWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL 480

Query: 481 AFVVYLVYRSISFSSWHNFINRKTYTASGN 511
           AFVVYLVYRSISF+SWHNFINRKTY  +GN
Sbjct: 481 AFVVYLVYRSISFTSWHNFINRKTYLGNGN 510

BLAST of Lsi10G001670 vs. ExPASy TrEMBL
Match: A0A6J1JDR7 (metal transporter Nramp3-like OS=Cucurbita maxima OX=3661 GN=LOC111483617 PE=3 SV=1)

HSP 1 Score: 921.0 bits (2379), Expect = 2.2e-264
Identity = 481/510 (94.31%), Postives = 499/510 (97.84%), Query Frame = 0

Query: 1   MHPDDQQQQPLIEEEDEETAYDLTEKVVVIGIDEAESDSDVGLLPFSWRKLWLFTGPGFL 60
           MHPDDQ+ QPL+EEEDEETAY+LTEKVVV+GIDEAESD DVGL PFSWRKLWLFTGPGFL
Sbjct: 1   MHPDDQRWQPLLEEEDEETAYNLTEKVVVVGIDEAESDLDVGLPPFSWRKLWLFTGPGFL 60

Query: 61  MSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120
           MSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATG+HLAELCRE
Sbjct: 61  MSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGKHLAELCRE 120

Query: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180
           EYP+WARI+LWVMAE ALIGADIQEVIGSAIAIKILSNGALPLWAGVIITA DCFIFLFL
Sbjct: 121 EYPSWARILLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITAFDCFIFLFL 180

Query: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240
           ENYGVRKLEAVFAVLIATMALSFAW+FGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC
Sbjct: 181 ENYGVRKLEAVFAVLIATMALSFAWIFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240

Query: 241 IIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF 300
           IIMPHNVFLHSALVQSRDV+PSKKGRV+EALRYYSIESTLALFVSFIINLFVTTVFAKAF
Sbjct: 241 IIMPHNVFLHSALVQSRDVNPSKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF 300

Query: 301 YGTDIASSIGLVNAGQYLQDKYGGGIFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360
           YGTDIASSIGLVNAGQYLQDKYGGG+FPILYIWAIGLLAAGQSSTITGTYAGQFIM GFL
Sbjct: 301 YGTDIASSIGLVNAGQYLQDKYGGGLFPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL 360

Query: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420
           NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL
Sbjct: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420

Query: 421 ASKEHLMGSFKIGPVLKTISWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL 480
           ASKEHLMGSF+IGP+LK  SWLVAVLVMAINGYLLVSFFSSEVNGV++AIFVFVFIAAYL
Sbjct: 421 ASKEHLMGSFRIGPILKLSSWLVAVLVMAINGYLLVSFFSSEVNGVIVAIFVFVFIAAYL 480

Query: 481 AFVVYLVYRSISFSSWHNFINRKTYTASGN 511
           +FVVYLVYRSISFSSWH+FINRKT T + N
Sbjct: 481 SFVVYLVYRSISFSSWHSFINRKTSTDNEN 510

BLAST of Lsi10G001670 vs. NCBI nr
Match: XP_038901577.1 (metal transporter Nramp3-like [Benincasa hispida])

HSP 1 Score: 947.2 bits (2447), Expect = 5.8e-272
Identity = 496/510 (97.25%), Postives = 504/510 (98.82%), Query Frame = 0

Query: 1   MHPDDQQQQPLIEEEDEETAYDLTEKVVVIGIDEAESDSDVGLLPFSWRKLWLFTGPGFL 60
           MHPDDQQQQPLIEEEDEETAY++TEKVVVIGI+E ESDSDVGLLPFSWRKLWLFTGPGFL
Sbjct: 1   MHPDDQQQQPLIEEEDEETAYNVTEKVVVIGINEPESDSDVGLLPFSWRKLWLFTGPGFL 60

Query: 61  MSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120
           MSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE
Sbjct: 61  MSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120

Query: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180
           EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL
Sbjct: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180

Query: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240
           ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTI QAVAVVGC
Sbjct: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTITQAVAVVGC 240

Query: 241 IIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF 300
           IIMPHNVFLHSALVQSRDVDPSKKGRV+EALRYYSIESTLALFVSFIINLFVTTVFAKAF
Sbjct: 241 IIMPHNVFLHSALVQSRDVDPSKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF 300

Query: 301 YGTDIASSIGLVNAGQYLQDKYGGGIFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360
           YGTDIA+SIGLVNAGQYLQDKYGGG+FPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL
Sbjct: 301 YGTDIANSIGLVNAGQYLQDKYGGGLFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360

Query: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420
           NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL
Sbjct: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420

Query: 421 ASKEHLMGSFKIGPVLKTISWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL 480
           ASKEHLMGSFKIGP+LKT SWLVAVLVMAINGYLLVSF SSEVNGVL  IFVFVFIAAYL
Sbjct: 421 ASKEHLMGSFKIGPILKTTSWLVAVLVMAINGYLLVSFISSEVNGVLFTIFVFVFIAAYL 480

Query: 481 AFVVYLVYRSISFSSWHNFINRKTYTASGN 511
           AFVVYLVYRSISFSSWHNFINRKTYTA+GN
Sbjct: 481 AFVVYLVYRSISFSSWHNFINRKTYTANGN 510

BLAST of Lsi10G001670 vs. NCBI nr
Match: KAA0032521.1 (metal transporter Nramp3-like [Cucumis melo var. makuwa])

HSP 1 Score: 946.4 bits (2445), Expect = 1.0e-271
Identity = 494/510 (96.86%), Postives = 505/510 (99.02%), Query Frame = 0

Query: 1   MHPDDQQQQPLIEEEDEETAYDLTEKVVVIGIDEAESDSDVGLLPFSWRKLWLFTGPGFL 60
           MHPDDQQQQPLIEEE+EETAYDLTEKVVVIGIDE ESDSDVGLLPFSWRKLWLFTGPGFL
Sbjct: 1   MHPDDQQQQPLIEEEEEETAYDLTEKVVVIGIDETESDSDVGLLPFSWRKLWLFTGPGFL 60

Query: 61  MSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120
           MSIAFLDPGNLESNLQAGA AGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE
Sbjct: 61  MSIAFLDPGNLESNLQAGATAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120

Query: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180
           EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL
Sbjct: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180

Query: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240
           ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC
Sbjct: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240

Query: 241 IIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF 300
           IIMPHNVFLHSALVQSRD++PSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF
Sbjct: 241 IIMPHNVFLHSALVQSRDINPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF 300

Query: 301 YGTDIASSIGLVNAGQYLQDKYGGGIFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360
           YGTDIA+SIGLVNAG+YL+DKYGGG+FPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL
Sbjct: 301 YGTDIANSIGLVNAGKYLEDKYGGGLFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360

Query: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420
           NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL
Sbjct: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420

Query: 421 ASKEHLMGSFKIGPVLKTISWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL 480
           ASKEHLMG+F+IGPVLKT SWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL
Sbjct: 421 ASKEHLMGTFRIGPVLKTTSWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL 480

Query: 481 AFVVYLVYRSISFSSWHNFINRKTYTASGN 511
           AFVVYLVYRSISF+SWHNFINRKTY  +GN
Sbjct: 481 AFVVYLVYRSISFTSWHNFINRKTYLGNGN 510

BLAST of Lsi10G001670 vs. NCBI nr
Match: XP_004151871.1 (metal transporter Nramp3 [Cucumis sativus] >KGN63290.1 hypothetical protein Csa_022482 [Cucumis sativus])

HSP 1 Score: 944.9 bits (2441), Expect = 2.9e-271
Identity = 493/510 (96.67%), Postives = 503/510 (98.63%), Query Frame = 0

Query: 1   MHPDDQQQQPLIEEEDEETAYDLTEKVVVIGIDEAESDSDVGLLPFSWRKLWLFTGPGFL 60
           MHPDDQQQQPLIEEE+EETAYDLTEKVVVIGIDE ESDSDVGLLPFSWRKLWLFTGPGFL
Sbjct: 1   MHPDDQQQQPLIEEEEEETAYDLTEKVVVIGIDETESDSDVGLLPFSWRKLWLFTGPGFL 60

Query: 61  MSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120
           MSIAFLDPGNLESNLQAGA AGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE
Sbjct: 61  MSIAFLDPGNLESNLQAGATAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120

Query: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180
           EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGV ITALDCFIFLFL
Sbjct: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVTITALDCFIFLFL 180

Query: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240
           ENYGVRKLEAVFAVLIATMALSFAWMFG+TKPDGKELLLGILIPKLSSKTIKQAVAVVGC
Sbjct: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGDTKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240

Query: 241 IIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF 300
           IIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLAL VSFIINLFVTTVFAKAF
Sbjct: 241 IIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLALLVSFIINLFVTTVFAKAF 300

Query: 301 YGTDIASSIGLVNAGQYLQDKYGGGIFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360
           YGTDIA+SIGLVNAGQYLQDKYGGG+FPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL
Sbjct: 301 YGTDIANSIGLVNAGQYLQDKYGGGLFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360

Query: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420
           NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL
Sbjct: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420

Query: 421 ASKEHLMGSFKIGPVLKTISWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL 480
           ASKEHLMG+F+IGPVLKT SWLVAVLVMAINGYLLVSFFSSEVNGV++AIFVFVFIAAYL
Sbjct: 421 ASKEHLMGTFRIGPVLKTTSWLVAVLVMAINGYLLVSFFSSEVNGVVVAIFVFVFIAAYL 480

Query: 481 AFVVYLVYRSISFSSWHNFINRKTYTASGN 511
           AFVVYLVYRSISF+SWHNFINRKTY  +GN
Sbjct: 481 AFVVYLVYRSISFTSWHNFINRKTYVGNGN 510

BLAST of Lsi10G001670 vs. NCBI nr
Match: XP_008455821.1 (PREDICTED: metal transporter Nramp3-like [Cucumis melo] >TYJ98346.1 metal transporter Nramp3-like [Cucumis melo var. makuwa])

HSP 1 Score: 944.5 bits (2440), Expect = 3.8e-271
Identity = 493/510 (96.67%), Postives = 505/510 (99.02%), Query Frame = 0

Query: 1   MHPDDQQQQPLIEEEDEETAYDLTEKVVVIGIDEAESDSDVGLLPFSWRKLWLFTGPGFL 60
           MHPDDQQQQPLIEEE+EETAYDLTEKVVVIGIDE ESDSDVGLLPFSWRKLWLFTGPGFL
Sbjct: 1   MHPDDQQQQPLIEEEEEETAYDLTEKVVVIGIDETESDSDVGLLPFSWRKLWLFTGPGFL 60

Query: 61  MSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120
           MSIAFLDPGNLESNLQAGA AGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE
Sbjct: 61  MSIAFLDPGNLESNLQAGATAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120

Query: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180
           EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL
Sbjct: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180

Query: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240
           ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC
Sbjct: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240

Query: 241 IIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF 300
           IIMPHNVFLHSALVQSRD++PSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF
Sbjct: 241 IIMPHNVFLHSALVQSRDINPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF 300

Query: 301 YGTDIASSIGLVNAGQYLQDKYGGGIFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360
           YGT+IA+SIGLVNAG+YL+DKYGGG+FPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL
Sbjct: 301 YGTNIANSIGLVNAGKYLEDKYGGGLFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360

Query: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420
           NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL
Sbjct: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420

Query: 421 ASKEHLMGSFKIGPVLKTISWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL 480
           ASKEHLMG+F+IGPVLKT SWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL
Sbjct: 421 ASKEHLMGTFRIGPVLKTTSWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL 480

Query: 481 AFVVYLVYRSISFSSWHNFINRKTYTASGN 511
           AFVVYLVYRSISF+SWHNFINRKTY  +GN
Sbjct: 481 AFVVYLVYRSISFTSWHNFINRKTYLGNGN 510

BLAST of Lsi10G001670 vs. NCBI nr
Match: XP_023512741.1 (metal transporter Nramp3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 922.5 bits (2383), Expect = 1.5e-264
Identity = 482/510 (94.51%), Postives = 499/510 (97.84%), Query Frame = 0

Query: 1   MHPDDQQQQPLIEEEDEETAYDLTEKVVVIGIDEAESDSDVGLLPFSWRKLWLFTGPGFL 60
           MHPDDQ++QPL+EEEDEETAY+LTEKVVV+GIDEAESD DVGL PFSWRKLWLFTGPGFL
Sbjct: 1   MHPDDQRRQPLLEEEDEETAYNLTEKVVVVGIDEAESDLDVGLPPFSWRKLWLFTGPGFL 60

Query: 61  MSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 120
           MSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATG+HLAELCRE
Sbjct: 61  MSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGKHLAELCRE 120

Query: 121 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 180
           EYP+WARI+LWVMAE ALIGADIQEVIGSAIAIKILSNGALPLWAGVIITA DCFIFLFL
Sbjct: 121 EYPSWARILLWVMAELALIGADIQEVIGSAIAIKILSNGALPLWAGVIITAFDCFIFLFL 180

Query: 181 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240
           ENYGVRKLEAVFAVLIATMALSFAW+FGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC
Sbjct: 181 ENYGVRKLEAVFAVLIATMALSFAWIFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 240

Query: 241 IIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF 300
           IIMPHNVFLHSALVQSRDV+PSKKGRV+EALRYYSIESTLALFVSFIINLFVTTVFAKAF
Sbjct: 241 IIMPHNVFLHSALVQSRDVNPSKKGRVREALRYYSIESTLALFVSFIINLFVTTVFAKAF 300

Query: 301 YGTDIASSIGLVNAGQYLQDKYGGGIFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 360
           YGTDIASSIGLVNAGQYLQDKYGGG+FPILYIWAIGLLAAGQSSTITGTYAGQFIM GFL
Sbjct: 301 YGTDIASSIGLVNAGQYLQDKYGGGLFPILYIWAIGLLAAGQSSTITGTYAGQFIMGGFL 360

Query: 361 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420
           NLKLKKWLRALITRSFAIVPTMIVAL FETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL
Sbjct: 361 NLKLKKWLRALITRSFAIVPTMIVALAFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 420

Query: 421 ASKEHLMGSFKIGPVLKTISWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL 480
           ASKEHLMGSF+IGP+LK  SWLVAVLVMAINGYLLVSFFSSEVNGVL+AIFVFVFIAAYL
Sbjct: 421 ASKEHLMGSFRIGPILKLSSWLVAVLVMAINGYLLVSFFSSEVNGVLVAIFVFVFIAAYL 480

Query: 481 AFVVYLVYRSISFSSWHNFINRKTYTASGN 511
           +FVVYLVYRSISFSSWH+FINRKT T S N
Sbjct: 481 SFVVYLVYRSISFSSWHSFINRKTSTDSEN 510

BLAST of Lsi10G001670 vs. TAIR 10
Match: AT2G23150.1 (natural resistance-associated macrophage protein 3 )

HSP 1 Score: 753.8 bits (1945), Expect = 8.8e-218
Identity = 387/501 (77.25%), Postives = 448/501 (89.42%), Query Frame = 0

Query: 3   PDDQQQQPLI--EEEDEETAYDLTEKVVVIGIDEAESDSDVGL-----LPFSWRKLWLFT 62
           P  +  +PL+  EEE+EETAYD TEKV ++  +E E D + G+      PFSW+KLWLFT
Sbjct: 2   PQLENNEPLLINEEEEEETAYDETEKVHIVR-NEEEDDLEHGVGCGGAPPFSWKKLWLFT 61

Query: 63  GPGFLMSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLA 122
           GPGFLMSIAFLDPGNLE +LQAGA+AGYSLLWLL+WATAMGLL+QLLSARLGVATGRHLA
Sbjct: 62  GPGFLMSIAFLDPGNLEGDLQAGAVAGYSLLWLLMWATAMGLLVQLLSARLGVATGRHLA 121

Query: 123 ELCREEYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCF 182
           ELCR+EYPTWAR+VLWVMAE ALIG+DIQEVIGSAIAIKILSNG LPLWAGV+ITALDCF
Sbjct: 122 ELCRDEYPTWARMVLWVMAELALIGSDIQEVIGSAIAIKILSNGILPLWAGVVITALDCF 181

Query: 183 IFLFLENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAV 242
           +FLFLENYG+RKLEAVFAVLIATM +SFAWMFG+ KP G ELL+GIL+PKLSS+TI++AV
Sbjct: 182 VFLFLENYGIRKLEAVFAVLIATMGVSFAWMFGQAKPSGSELLIGILVPKLSSRTIQKAV 241

Query: 243 AVVGCIIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTV 302
            VVGCIIMPHNVFLHSALVQSR+VD  +K RVQEAL YY+IEST+ALF+SF+INLFVTTV
Sbjct: 242 GVVGCIIMPHNVFLHSALVQSREVDKRQKYRVQEALNYYTIESTIALFISFLINLFVTTV 301

Query: 303 FAKAFYGTDIASSIGLVNAGQYLQDKYGGGIFPILYIWAIGLLAAGQSSTITGTYAGQFI 362
           FAK FY TD+A+SIGLVNAGQYLQ+KYGGG+FPILYIWAIGLLAAGQSSTITGTYAGQFI
Sbjct: 302 FAKGFYNTDLANSIGLVNAGQYLQEKYGGGVFPILYIWAIGLLAAGQSSTITGTYAGQFI 361

Query: 363 MSGFLNLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALI 422
           M GFLN K+KKWLRALITRS AI+PT+IVALVF++S++ +DVLNEWLNVLQSIQIPFALI
Sbjct: 362 MGGFLNFKMKKWLRALITRSCAIIPTIIVALVFDSSEATLDVLNEWLNVLQSIQIPFALI 421

Query: 423 PLLCLASKEHLMGSFKIGPVLKTISWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVF 482
           PLLCL SKE +MGSFKIGP+ KTI+WLVA LV+ INGYLL+ FFS+EV+G++   FV +F
Sbjct: 422 PLLCLVSKEQIMGSFKIGPLYKTIAWLVAALVIMINGYLLLEFFSNEVSGIVYTGFVTLF 481

Query: 483 IAAYLAFVVYLVYRSISFSSW 497
            A+Y AF++YL+ R I+F+ W
Sbjct: 482 TASYGAFILYLIARGITFTPW 501

BLAST of Lsi10G001670 vs. TAIR 10
Match: AT5G67330.1 (natural resistance associated macrophage protein 4 )

HSP 1 Score: 746.9 bits (1927), Expect = 1.1e-215
Identity = 381/497 (76.66%), Postives = 444/497 (89.34%), Query Frame = 0

Query: 4   DDQQQQPLIEEEDEETAYDLTEKVVVIGIDE---AESDSDVGLLP-FSWRKLWLFTGPGF 63
           +  +++PL+    EE AY+ TEKV+++GIDE   A+ D D G  P FSW+KLWLFTGPGF
Sbjct: 3   ETDRERPLL--ASEERAYEETEKVLIVGIDEEEDADYDDDPGNSPKFSWKKLWLFTGPGF 62

Query: 64  LMSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCR 123
           LMSIAFLDPGNLES+LQAGAIAGYSL+WLL+WATA+GLLIQLLSARLGVATGRHLAELCR
Sbjct: 63  LMSIAFLDPGNLESDLQAGAIAGYSLIWLLMWATAIGLLIQLLSARLGVATGRHLAELCR 122

Query: 124 EEYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLF 183
           EEYPTWAR+VLW+MAE ALIGADIQEVIGSAIAIKILSNG +PLWAGV+ITALDCFIFLF
Sbjct: 123 EEYPTWARMVLWIMAEIALIGADIQEVIGSAIAIKILSNGLVPLWAGVVITALDCFIFLF 182

Query: 184 LENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVG 243
           LENYG+RKLEAVFA+LIATMAL+FAWMFG+TKP G ELL+G L+PKLSS+TIKQAV +VG
Sbjct: 183 LENYGIRKLEAVFAILIATMALAFAWMFGQTKPSGTELLVGALVPKLSSRTIKQAVGIVG 242

Query: 244 CIIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKA 303
           CIIMPHNVFLHSALVQSR+VDP K+ RV+EAL+YYSIEST AL VSFIIN+FVTTVFAK+
Sbjct: 243 CIIMPHNVFLHSALVQSREVDPKKRFRVKEALKYYSIESTGALAVSFIINVFVTTVFAKS 302

Query: 304 FYGTDIASSIGLVNAGQYLQDKYGGGIFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGF 363
           FYGT+IA +IGL NAGQYLQDKYGGG FPILYIWAIG+LAAGQSSTITGTYAGQFIM GF
Sbjct: 303 FYGTEIADTIGLANAGQYLQDKYGGGFFPILYIWAIGVLAAGQSSTITGTYAGQFIMGGF 362

Query: 364 LNLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLC 423
           LNLK+KKW+RALITRS AI+PTMIVALVF++SDSM+D LNEWLNVLQS+QIPFA+IPLLC
Sbjct: 363 LNLKMKKWVRALITRSCAIIPTMIVALVFDSSDSMLDELNEWLNVLQSVQIPFAVIPLLC 422

Query: 424 LASKEHLMGSFKIGPVLKTISWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAY 483
           L S E +MGSFKI P+++TISW+VA LV+AINGYL+V FFS     +++ + V +F  AY
Sbjct: 423 LVSNEQIMGSFKIQPLVQTISWIVAALVIAINGYLMVDFFSGAATNLILLVPVIIFAIAY 482

Query: 484 LAFVVYLVYRSISFSSW 497
           + FV+YL+ R ++++ W
Sbjct: 483 VVFVLYLISRGLTYTPW 497

BLAST of Lsi10G001670 vs. TAIR 10
Match: AT1G47240.1 (NRAMP metal ion transporter 2 )

HSP 1 Score: 716.5 bits (1848), Expect = 1.6e-206
Identity = 371/495 (74.95%), Postives = 425/495 (85.86%), Query Frame = 0

Query: 3   PDDQQQQPLIEEEDEETAYDLTEKVVVIGIDEAESDSDVGLL--PFSWRKLWLFTGPGFL 62
           P   Q  P  + E  E A++  EK++++   E+  D   G    PFSWRKLWLFTGPGFL
Sbjct: 20  PPPSQSLPSTDSE-SEAAFETNEKILIVDF-ESPDDPTTGDTPPPFSWRKLWLFTGPGFL 79

Query: 63  MSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCRE 122
           MSIAFLDPGNLE +LQAGAIAGYSLLWLL+WATAMGLLIQ+LSAR+GVATGRHLAELCR+
Sbjct: 80  MSIAFLDPGNLEGDLQAGAIAGYSLLWLLMWATAMGLLIQMLSARVGVATGRHLAELCRD 139

Query: 123 EYPTWARIVLWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFL 182
           EYPTWAR VLW MAE ALIGADIQEVIGSAIAI+ILS G LPLWAGV+ITA DCF+FLFL
Sbjct: 140 EYPTWARYVLWSMAELALIGADIQEVIGSAIAIQILSRGFLPLWAGVVITASDCFLFLFL 199

Query: 183 ENYGVRKLEAVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGC 242
           ENYGVRKLEAVFAVLIATM LSFAWMFGETKP GKEL++GIL+P+LSSKTI+QAV VVGC
Sbjct: 200 ENYGVRKLEAVFAVLIATMGLSFAWMFGETKPSGKELMIGILLPRLSSKTIRQAVGVVGC 259

Query: 243 IIMPHNVFLHSALVQSRDVDPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAF 302
           +IMPHNVFLHSALVQSR +DP +K RVQEAL YY IES++ALF+SF+INLFVTTVFAK F
Sbjct: 260 VIMPHNVFLHSALVQSRKIDPKRKSRVQEALNYYLIESSVALFISFMINLFVTTVFAKGF 319

Query: 303 YGTDIASSIGLVNAGQYLQDKYGGGIFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFL 362
           YGT+ A++IGLVNAGQYLQ+K+GGG+ PILYIW IGLLAAGQSSTITGTYAGQFIM GFL
Sbjct: 320 YGTEKANNIGLVNAGQYLQEKFGGGLLPILYIWGIGLLAAGQSSTITGTYAGQFIMGGFL 379

Query: 363 NLKLKKWLRALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCL 422
           NL+LKKW+RA+ITRS AIVPTMIVA+VF TS++ +DVLNEWLNVLQS+QIPFAL+PLL L
Sbjct: 380 NLRLKKWMRAVITRSCAIVPTMIVAIVFNTSEASLDVLNEWLNVLQSVQIPFALLPLLTL 439

Query: 423 ASKEHLMGSFKIGPVLKTISWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYL 482
            SKE +MG FKIGP+L+ I+W VA LVM INGYLL+ FF SEV+G L  + V V+  AY+
Sbjct: 440 VSKEEIMGDFKIGPILQRIAWTVAALVMIINGYLLLDFFVSEVDGFLFGVTVCVWTTAYI 499

Query: 483 AFVVYLVYRSISFSS 496
           AF+VYL+  S  F S
Sbjct: 500 AFIVYLISHSNFFPS 512

BLAST of Lsi10G001670 vs. TAIR 10
Match: AT4G18790.1 (NRAMP metal ion transporter family protein )

HSP 1 Score: 659.8 bits (1701), Expect = 1.7e-189
Identity = 334/488 (68.44%), Postives = 400/488 (81.97%), Query Frame = 0

Query: 10  PLIEEEDEETAYDLTEKVVVIGIDEAESDSDVGLLPFSWRKLWLFTGPGFLMSIAFLDPG 69
           P   + +E+  ++   +  ++ ++E    +   + PFSW KLW FTGPGFLMSIAFLDPG
Sbjct: 29  PETSQPEEDELHESPPENQILNVEEDRDKTYDSVPPFSWAKLWKFTGPGFLMSIAFLDPG 88

Query: 70  NLESNLQAGAIAGYSLLWLLLWATAMGLLIQLLSARLGVATGRHLAELCREEYPTWARIV 129
           N+E +LQAGA+AGYSLLWLLLWAT MGLL+QLLSAR+GVATGRHLAE+CR EYP+WARI+
Sbjct: 89  NIEGDLQAGAVAGYSLLWLLLWATLMGLLMQLLSARIGVATGRHLAEICRSEYPSWARIL 148

Query: 130 LWVMAEFALIGADIQEVIGSAIAIKILSNGALPLWAGVIITALDCFIFLFLENYGVRKLE 189
           LW MAE ALIGADIQEVIGSAIA++IL+ G LP+W GVIIT+ DCF+  +LE  G+RKLE
Sbjct: 149 LWFMAEVALIGADIQEVIGSAIALQILTRGFLPIWVGVIITSFDCFLISYLEKCGMRKLE 208

Query: 190 AVFAVLIATMALSFAWMFGETKPDGKELLLGILIPKLSSKTIKQAVAVVGCIIMPHNVFL 249
            +FAVLIATMALSFAWMF ETKP  +EL +GI+IPKL SKTI++AV VVGC+I PHNVFL
Sbjct: 209 GLFAVLIATMALSFAWMFNETKPSVEELFIGIIIPKLGSKTIREAVGVVGCVITPHNVFL 268

Query: 250 HSALVQSRDVDPSKKGRVQEALRYYSIESTLALFVSFIINLFVTTVFAKAFYGTDIASSI 309
           HSALVQSR  DP +  RVQEAL YY+IES+ ALFVSF+INLFVT VFAK FYGT  A SI
Sbjct: 269 HSALVQSRKTDPKEINRVQEALNYYTIESSAALFVSFMINLFVTAVFAKGFYGTKQADSI 328

Query: 310 GLVNAGQYLQDKYGGGIFPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFLNLKLKKWLR 369
           GLVNAG YLQ+KYGGG+FPILYIW IGLLAAGQSSTITGTYAGQFIM GFL+L++++WL 
Sbjct: 329 GLVNAGYYLQEKYGGGVFPILYIWGIGLLAAGQSSTITGTYAGQFIMEGFLDLQMEQWLS 388

Query: 370 ALITRSFAIVPTMIVALVFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCLASKEHLMGS 429
           A ITRSFAIVPTM VA++F TS+  +DVLNEWLN+LQS+QIPFA+IPLL + S EH+MG 
Sbjct: 389 AFITRSFAIVPTMFVAIMFNTSEGSLDVLNEWLNILQSMQIPFAVIPLLTMVSNEHIMGV 448

Query: 430 FKIGPVLKTISWLVAVLVMAINGYLLVSFFSSEVNGVLIAIFVFVFIAAYLAFVVYLV-Y 489
           FKIGP L+ ++W VAV VM INGYLL+ FF +EV G L+   VF  +  Y++F++YLV Y
Sbjct: 449 FKIGPSLEKLAWTVAVFVMMINGYLLLDFFMAEVEGFLVGFLVFGGVVGYISFIIYLVSY 508

Query: 490 RSISFSSW 497
           RS   SSW
Sbjct: 509 RSSQSSSW 516

BLAST of Lsi10G001670 vs. TAIR 10
Match: AT1G15960.1 (NRAMP metal ion transporter 6 )

HSP 1 Score: 330.5 bits (846), Expect = 2.4e-90
Identity = 209/483 (43.27%), Postives = 305/483 (63.15%), Query Frame = 0

Query: 37  SDSDVGLLP--FSWRKLWLFTGPGFLMSIAFLDPGNLESNLQAGAIAGYSLLWLLLWATA 96
           SDS+  L+P   SW+  + + GPGFL+SIA++DPGN E++LQ+GA   Y LLW++L A+ 
Sbjct: 22  SDSNQILVPEKKSWKNFFSYLGPGFLVSIAYIDPGNFETDLQSGAQYKYELLWIILVASC 81

Query: 97  MGLLIQLLSARLGVATGRHLAELCREEYPTWARIVLWVMAEFALIGADIQEVIGSAIAIK 156
             L+IQ L+A LGV TG+HLAE CR EY      +LWV+AE A++  DI EVIG+A A+ 
Sbjct: 82  AALVIQSLAANLGVVTGKHLAEHCRAEYSKVPNFMLWVVAEIAVVACDIPEVIGTAFALN 141

Query: 157 ILSNGALPLWAGVIITALDCFIFLFLENYGVRKLEAVFAVLIATMALSFAWMFGETKPDG 216
           +L N  +P+W GV++T L   I L L+ YG+RKLE + A L+ T+AL F      +KPD 
Sbjct: 142 MLFN--IPVWIGVLLTGLSTLILLALQQYGIRKLEFLIAFLVFTIALCFFVELHYSKPDP 201

Query: 217 KELLLGILIPKLSSK-TIKQAVAVVGCIIMPHNVFLHSALVQSRDVDPSKKGRVQEALRY 276
           KE+L G+ +P+L        A++++G ++MPHN+FLHSALV SR +  S  G ++EA RY
Sbjct: 202 KEVLYGLFVPQLKGNGATGLAISLLGAMVMPHNLFLHSALVLSRKIPRSVTG-IKEACRY 261

Query: 277 YSIESTLALFVSFIINLFVTTVFAKAFYGTDIA-------SSIGLVNAGQYLQDKYGGGI 336
           Y IES LAL V+F+IN+ V +V       +D++         + L  A   L++  G   
Sbjct: 262 YLIESGLALMVAFLINVSVISVSGAVCNASDLSPEDRASCQDLDLNKASFLLRNVVGKWS 321

Query: 337 FPILYIWAIGLLAAGQSSTITGTYAGQFIMSGFLNLKLKKWLRALITRSFAIVPTMIVAL 396
                ++AI LLA+GQSSTITGTYAGQ++M GFL+L+L+ WLR  +TR  AI+P++IVAL
Sbjct: 322 SK---LFAIALLASGQSSTITGTYAGQYVMQGFLDLRLEPWLRNFLTRCLAIIPSLIVAL 381

Query: 397 VFETSDSMVDVLNEWLNVLQSIQIPFALIPLLCLASKEHLMGSFKIGPVLKTISWLVAVL 456
           +  ++ +   ++    +++ S ++PFAL+PLL   S +  MGS     V+ +++W++  L
Sbjct: 382 IGGSAGAGKLII--IASMILSFELPFALVPLLKFTSSKTKMGSHANSLVISSVTWIIGGL 441

Query: 457 VMAINGYLLVSFF-----SSEVNGVLI---AIFVFVFIAAYLAFVVYLVYRSISFSSWHN 502
           +M IN Y LVS F      S +N V I    +  F  IA YLA + YLV R    SS  +
Sbjct: 442 IMGINIYYLVSSFIKLLLHSHMNLVAIVFLGVLGFSGIATYLAAISYLVLRKNRESSSTH 496

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SNV91.2e-21677.25Metal transporter Nramp3 OS=Arabidopsis thaliana OX=3702 GN=NRAMP3 PE=1 SV=2[more]
Q9FN181.5e-21476.66Metal transporter Nramp4 OS=Arabidopsis thaliana OX=3702 GN=NRAMP4 PE=2 SV=1[more]
Q9C6B22.2e-20574.95Metal transporter Nramp2 OS=Arabidopsis thaliana OX=3702 GN=NRAMP2 PE=2 SV=1[more]
Q2QN308.4e-19772.93Metal transporter Nramp6 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP6 PE=2... [more]
Q10Q651.2e-19071.46Metal transporter Nramp2 OS=Oryza sativa subsp. japonica OX=39947 GN=NRAMP2 PE=2... [more]
Match NameE-valueIdentityDescription
A0A5A7SSI54.8e-27296.86Metal transporter Nramp3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A0A0LN031.4e-27196.67Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423700 PE=3 SV=1[more]
A0A5D3BH321.8e-27196.67Metal transporter Nramp3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3C1X31.8e-27196.67metal transporter Nramp3-like OS=Cucumis melo OX=3656 GN=LOC103495920 PE=3 SV=1[more]
A0A6J1JDR72.2e-26494.31metal transporter Nramp3-like OS=Cucurbita maxima OX=3661 GN=LOC111483617 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_038901577.15.8e-27297.25metal transporter Nramp3-like [Benincasa hispida][more]
KAA0032521.11.0e-27196.86metal transporter Nramp3-like [Cucumis melo var. makuwa][more]
XP_004151871.12.9e-27196.67metal transporter Nramp3 [Cucumis sativus] >KGN63290.1 hypothetical protein Csa_... [more]
XP_008455821.13.8e-27196.67PREDICTED: metal transporter Nramp3-like [Cucumis melo] >TYJ98346.1 metal transp... [more]
XP_023512741.11.5e-26494.51metal transporter Nramp3-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G23150.18.8e-21877.25natural resistance-associated macrophage protein 3 [more]
AT5G67330.11.1e-21576.66natural resistance associated macrophage protein 4 [more]
AT1G47240.11.6e-20674.95NRAMP metal ion transporter 2 [more]
AT4G18790.11.7e-18968.44NRAMP metal ion transporter family protein [more]
AT1G15960.12.4e-9043.27NRAMP metal ion transporter 6 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001046NRAMP familyPRINTSPR00447NATRESASSCMPcoord: 369..386
score: 56.67
coord: 234..257
score: 67.67
coord: 130..156
score: 62.37
coord: 160..179
score: 57.0
coord: 341..360
score: 77.0
coord: 403..422
score: 57.4
coord: 185..206
score: 52.36
IPR001046NRAMP familyPFAMPF01566Nrampcoord: 72..433
e-value: 2.7E-123
score: 411.5
IPR001046NRAMP familyTIGRFAMTIGR01197TIGR01197coord: 49..427
e-value: 4.6E-129
score: 429.1
IPR001046NRAMP familyPANTHERPTHR11706SOLUTE CARRIER PROTEIN FAMILY 11 MEMBERcoord: 8..507
IPR001046NRAMP familyHAMAPMF_00221NRAMPcoord: 37..459
score: 38.186539
NoneNo IPR availablePANTHERPTHR11706:SF89METAL TRANSPORTER NRAMP3 PROTEINcoord: 8..507

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lsi10G001670.1Lsi10G001670.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070574 cadmium ion transmembrane transport
biological_process GO:0042742 defense response to bacterium
biological_process GO:0055072 iron ion homeostasis
biological_process GO:0071421 manganese ion transmembrane transport
biological_process GO:2000379 positive regulation of reactive oxygen species metabolic process
biological_process GO:0030001 metal ion transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005774 vacuolar membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015086 cadmium ion transmembrane transporter activity
molecular_function GO:0005384 manganese ion transmembrane transporter activity
molecular_function GO:0046873 metal ion transmembrane transporter activity