Lsi10G001460 (gene) Bottle gourd (USVL1VR-Ls) v1

Overview


Name	Lsi10G001460
Type	gene
Organism	Lagenaria siceraria (Bottle gourd (USVL1VR-Ls) v1)
Description	Isocitrate lyase
Location	chr10: 2379737 .. 2381980 (-)
RNA-Seq Expression	Lsi10G001460
Synteny	Lsi10G001460

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTATTCTATTCAGAAAGATGAGAGAAAGACAAGTATGGCAGCATAGAGAGGGATGGGTTTCCCTTACGTGCCCTGCAAATGCACCTCACAACAGACAAGTTTCCATCAACCTCTGGAGATAACAATGAACAAAAAATCAAAATGTATTTTCCTTCAAAATCCACTTATACATATTTTATTGGTTTCTGTAGCTTTCTTTTCTTATCTAAATCCATAATTAAATTCACTTTTACCCATCTCAAACTAGACTTTCCCCTTATAAATACGACCCCATCAACAAACTTCATGCATTCAGTTGAGCTTCTTTTCGTTATTGTCAAGGAGAAGGAAAACTAGTTCTATAAACAACAAAAAGAAGCAGTATAACTCAGTTCTAGCCATGGCTGCCTCCTTCTCTGTTCCTTCAATGGTTTTTACTTAATTTGAATGCAAACCCACTTGAATTTTGCTCTTAATTTTCAACTACACTAGGTGCTACTAATACTAATGCTTACAGTTTTGAGATTTCTTAATGGTACAGTTAATGGAAGAAGAAGGAAGATTTGAAGCAGAAGTTGCGGAGGTACAAGCATGGTGGAATTCAGAGAGGTTCAAGCTAACACGACGGCCGTACACGGCAAAAGATGTGGTGTCACTACGCGGCAGCCTGAGACAAAGCTATGCTTCAAATGAATTAGCTAAAAAGCTATGGCGAACCCTCAAAACCCACCAAGCCAATGGCACAGCCTCGAGAACATTCGGAGCTCTAGACCCTGTTCAAGTGACGATGATGGCTAAGCATTTGGACACCATTTATGTCTCCGGCTGGCAGTGTTCTTCAACTCATACCTCCACCAACGAACCAGGCCCGGACCTCGCCGATTACCCATACGACACTGTTCCGAACAAGGTCGAGCATCTGTTCTTCGCTCAACAGTACCATGATCGGAAACAGAGGGAAGCGAGGATGAGTATGAGTCGAGAGGAACGAGCCAAAACCCCTTATATTGACTATCTCAAACCGATTATCGCCGACGGTGACACCGGGTTCGGCGGCACCACCGCCACGGTGAAGCTCTGTAAGCTATTTGTGGAACGCGGTGCTGCCGGTGTTCACATTGAGGATCAGTCGTCGGTGACGAAGAAATGCGGTCATATGGCTGGGAAAGTGTTGGTGGCGGTTAGCGAGCACATCAATCGCCTCGTAGCGGCTCGATTGCAGTTCGATGTGATGGGGGTTGAGACGGTTCTCGTAGCTCGAACCGATGCAGTTGCAGCCACTTTGATTCAAACGAATGTGGATAAAAGAGATCATCAATTTATTTTGGGTGCGACGAACCCTAATTTGAGAGGGAAGAGTTTAGCTGGGGTTTTGGCAGAAGCCATGGCGGCTGGAAAACCAGGTGCGGAACTTCAAGCTCTAGAAGATCAATGGATTTCCATGGCGCAATTGAAGACATTTTCGGAATGTGTAACGGACGCAATCATGAATACAAACGCAACAGAGAGTGAAAAGAGGAGGAAATTAGACGAATGGATGAATCATTCCAGTTACGAGAAATGTATATCGAACGAACAAGGTCGAGAAATCGCAGAGAAATTAGGGCTGAAGAATCTCTTCTGGGATTGGGATTTGCCCAGAACCAGAGAAGGGTTTTACAGGTTCAAAGGTTCGGTAATGGCGGCGATTGTTCGTGGTTGGGCTTTCGCACCACATGCAGATCTAATCTGGATGGAAACTTCAAGCCCGGATTTAGTCGAATGCACAACATTCGCAAAAGGGGTGAAATCGACACACCCAGAAACAATGTTGGCTTATAATCTATCGCCATCTTTCAACTGGGATGCATCAGGAATGAGCGATAAACAAATGGAAGAGTTCATCCCGAGGATCGCGAGGCTCGGGTTCTGCTGGCAGTTCATAACGCTGGCCGGATTCCACGCAGATGCGCTGGTGATCGACACATTTGCGAGAGATTACGCGAGAAGGGGAATGTTGGCTTATGTAGAGAGAATCCAGAGAGAAGAAAGAAGCAATGGAGTGGACACATTGGCTCATCAGAAATGGTCCGGTGCTAACTACTACGATCGGTATCTGAAGACGGTTCAAGGCGGGATTTCGTCGACGGCCGCCATGGGAAAAGGCAAGCAAATTCATCATTATTGGATTGATATTACGAATTATGAATTTATAAAGTGTGTTTATGAAGTGTTTTGAATATTTGAAATGCAGGGGTTACAGAGGAGCAGTTCAAGGAGTC

mRNA sequence

ATGGCTATTCTATTCAGAAAGATGAGAGAAAGACAATTAATGGAAGAAGAAGGAAGATTTGAAGCAGAAGTTGCGGAGGTACAAGCATGGTGGAATTCAGAGAGGTTCAAGCTAACACGACGGCCGTACACGGCAAAAGATGTGGTGTCACTACGCGGCAGCCTGAGACAAAGCTATGCTTCAAATGAATTAGCTAAAAAGCTATGGCGAACCCTCAAAACCCACCAAGCCAATGGCACAGCCTCGAGAACATTCGGAGCTCTAGACCCTGTTCAAGTGACGATGATGGCTAAGCATTTGGACACCATTTATGTCTCCGGCTGGCAGTGTTCTTCAACTCATACCTCCACCAACGAACCAGGCCCGGACCTCGCCGATTACCCATACGACACTGTTCCGAACAAGGTCGAGCATCTGTTCTTCGCTCAACAGTACCATGATCGGAAACAGAGGGAAGCGAGGATGAGTATGAGTCGAGAGGAACGAGCCAAAACCCCTTATATTGACTATCTCAAACCGATTATCGCCGACGGTGACACCGGGTTCGGCGGCACCACCGCCACGGTGAAGCTCTGTAAGCTATTTGTGGAACGCGGTGCTGCCGGTGTTCACATTGAGGATCAGTCGTCGGTGACGAAGAAATGCGGTCATATGGCTGGGAAAGTGTTGGTGGCGGTTAGCGAGCACATCAATCGCCTCGTAGCGGCTCGATTGCAGTTCGATGTGATGGGGGTTGAGACGGTTCTCGTAGCTCGAACCGATGCAGTTGCAGCCACTTTGATTCAAACGAATGTGGATAAAAGAGATCATCAATTTATTTTGGGTGCGACGAACCCTAATTTGAGAGGGAAGAGTTTAGCTGGGGTTTTGGCAGAAGCCATGGCGGCTGGAAAACCAGGTGCGGAACTTCAAGCTCTAGAAGATCAATGGATTTCCATGGCGCAATTGAAGACATTTTCGGAATGTGTAACGGACGCAATCATGAATACAAACGCAACAGAGAGTGAAAAGAGGAGGAAATTAGACGAATGGATGAATCATTCCAGTTACGAGAAATGTATATCGAACGAACAAGGTCGAGAAATCGCAGAGAAATTAGGGCTGAAGAATCTCTTCTGGGATTGGGATTTGCCCAGAACCAGAGAAGGGTTTTACAGGTTCAAAGGTTCGGTAATGGCGGCGATTGTTCGTGGTTGGGCTTTCGCACCACATGCAGATCTAATCTGGATGGAAACTTCAAGCCCGGATTTAGTCGAATGCACAACATTCGCAAAAGGGGTGAAATCGACACACCCAGAAACAATGTTGGCTTATAATCTATCGCCATCTTTCAACTGGGATGCATCAGGAATGAGCGATAAACAAATGGAAGAGTTCATCCCGAGGATCGCGAGGCTCGGGTTCTGCTGGCAGTTCATAACGCTGGCCGGATTCCACGCAGATGCGCTGGTGATCGACACATTTGCGAGAGATTACGCGAGAAGGGGAATGTTGGCTTATGTAGAGAGAATCCAGAGAGAAGAAAGAAGCAATGGAGTGGACACATTGGCTCATCAGAAATGGTCCGGTGCTAACTACTACGATCGGTATCTGAAGACGGTTCAAGGCGGGATTTCGTCGACGGCCGCCATGGGAAAAGGCAAGCAAATTCATCATTATTGGATTGATATTACGAATTATGAATTTATAAAGTGTGTTTATGAAGTGTTTTGAATATTTGAAATGCAGGGGTTACAGAGGAGCAGTTCAAGGAGTC

Coding sequence (CDS)

ATGGCTATTCTATTCAGAAAGATGAGAGAAAGACAATTAATGGAAGAAGAAGGAAGATTTGAAGCAGAAGTTGCGGAGGTACAAGCATGGTGGAATTCAGAGAGGTTCAAGCTAACACGACGGCCGTACACGGCAAAAGATGTGGTGTCACTACGCGGCAGCCTGAGACAAAGCTATGCTTCAAATGAATTAGCTAAAAAGCTATGGCGAACCCTCAAAACCCACCAAGCCAATGGCACAGCCTCGAGAACATTCGGAGCTCTAGACCCTGTTCAAGTGACGATGATGGCTAAGCATTTGGACACCATTTATGTCTCCGGCTGGCAGTGTTCTTCAACTCATACCTCCACCAACGAACCAGGCCCGGACCTCGCCGATTACCCATACGACACTGTTCCGAACAAGGTCGAGCATCTGTTCTTCGCTCAACAGTACCATGATCGGAAACAGAGGGAAGCGAGGATGAGTATGAGTCGAGAGGAACGAGCCAAAACCCCTTATATTGACTATCTCAAACCGATTATCGCCGACGGTGACACCGGGTTCGGCGGCACCACCGCCACGGTGAAGCTCTGTAAGCTATTTGTGGAACGCGGTGCTGCCGGTGTTCACATTGAGGATCAGTCGTCGGTGACGAAGAAATGCGGTCATATGGCTGGGAAAGTGTTGGTGGCGGTTAGCGAGCACATCAATCGCCTCGTAGCGGCTCGATTGCAGTTCGATGTGATGGGGGTTGAGACGGTTCTCGTAGCTCGAACCGATGCAGTTGCAGCCACTTTGATTCAAACGAATGTGGATAAAAGAGATCATCAATTTATTTTGGGTGCGACGAACCCTAATTTGAGAGGGAAGAGTTTAGCTGGGGTTTTGGCAGAAGCCATGGCGGCTGGAAAACCAGGTGCGGAACTTCAAGCTCTAGAAGATCAATGGATTTCCATGGCGCAATTGAAGACATTTTCGGAATGTGTAACGGACGCAATCATGAATACAAACGCAACAGAGAGTGAAAAGAGGAGGAAATTAGACGAATGGATGAATCATTCCAGTTACGAGAAATGTATATCGAACGAACAAGGTCGAGAAATCGCAGAGAAATTAGGGCTGAAGAATCTCTTCTGGGATTGGGATTTGCCCAGAACCAGAGAAGGGTTTTACAGGTTCAAAGGTTCGGTAATGGCGGCGATTGTTCGTGGTTGGGCTTTCGCACCACATGCAGATCTAATCTGGATGGAAACTTCAAGCCCGGATTTAGTCGAATGCACAACATTCGCAAAAGGGGTGAAATCGACACACCCAGAAACAATGTTGGCTTATAATCTATCGCCATCTTTCAACTGGGATGCATCAGGAATGAGCGATAAACAAATGGAAGAGTTCATCCCGAGGATCGCGAGGCTCGGGTTCTGCTGGCAGTTCATAACGCTGGCCGGATTCCACGCAGATGCGCTGGTGATCGACACATTTGCGAGAGATTACGCGAGAAGGGGAATGTTGGCTTATGTAGAGAGAATCCAGAGAGAAGAAAGAAGCAATGGAGTGGACACATTGGCTCATCAGAAATGGTCCGGTGCTAACTACTACGATCGGTATCTGAAGACGGTTCAAGGCGGGATTTCGTCGACGGCCGCCATGGGAAAAGGCAAGCAAATTCATCATTATTGGATTGATATTACGAATTATGAATTTATAAAGTGTGTTTATGAAGTGTTTTGA

Protein sequence

MAILFRKMRERQLMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNELAKKLWRTLKTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTVPNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVARTDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGKPGAELQALEDQWISMAQLKTFSECVTDAIMNTNATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLFWDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSTHPETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVIDTFARDYARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGKQIHHYWIDITNYEFIKCVYEVF

Homology

[Close]

BLAST of Lsi10G001460 vs. ExPASy Swiss-Prot
Match: P49296 (Isocitrate lyase OS=Cucumis sativus OX=3659 PE=3 SV=1)

HSP 1 Score: 1071.6 bits (2770), Expect = 2.9e-312
Identity = 525/535 (98.13%), Postives = 532/535 (99.44%), Query Frame = 0


Query: 13  LMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNELAKKLWRTL 72
           +MEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 73  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 132
           KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 133 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 192
           PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 190

Query: 193 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 252
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVET+LVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETILVAR 250

Query: 253 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGKPGAELQALEDQWIS 312
           TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAG LAEAMAAGK GAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGALAEAMAAGKTGAELQALEDQWIS 310

Query: 313 MAQLKTFSECVTDAIMNTNATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 372
           MAQLKTFSECVTDAIMNTNATE+EKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 373 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSTHP 432
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKG+KS HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGMKSIHP 430

Query: 433 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVIDTFARDY 492
           ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALV+DTFARDY
Sbjct: 431 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 490

Query: 493 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 548
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 545

[Close]

BLAST of Lsi10G001460 vs. ExPASy Swiss-Prot
Match: P93110 (Isocitrate lyase OS=Cucurbita maxima OX=3661 PE=2 SV=1)

HSP 1 Score: 1062.8 bits (2747), Expect = 1.4e-309
Identity = 522/535 (97.57%), Postives = 528/535 (98.69%), Query Frame = 0


Query: 13  LMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNELAKKLWRTL 72
           +MEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 73  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 132
           KTHQAN TASRTFGALDPVQVTMMAKHLD+IYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANSTASRTFGALDPVQVTMMAKHLDSIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 133 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 192
           PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPY+DYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGFGGTTATVKLC 190

Query: 193 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 252
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 250

Query: 253 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGKPGAELQALEDQWIS 312
           TDAVAATLIQTNVD RDHQFILGATNPNLRGKSLAGVLAEAMAAGK GAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDSRDHQFILGATNPNLRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 310

Query: 313 MAQLKTFSECVTDAIMNTNATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 372
           MAQLKTFSECVTDAIMN+N TESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNSNGTESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 373 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSTHP 432
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKS HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSVHP 430

Query: 433 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVIDTFARDY 492
           E MLAYNLSPSFNWDASGMSDKQMEEFIP IARLGFCWQFITLAGFHADALVIDTFARDY
Sbjct: 431 EIMLAYNLSPSFNWDASGMSDKQMEEFIPTIARLGFCWQFITLAGFHADALVIDTFARDY 490

Query: 493 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 548
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 545

[Close]

BLAST of Lsi10G001460 vs. ExPASy Swiss-Prot
Match: P17069 (Isocitrate lyase OS=Gossypium hirsutum OX=3635 PE=2 SV=1)

HSP 1 Score: 998.8 bits (2581), Expect = 2.5e-290
Identity = 479/535 (89.53%), Postives = 514/535 (96.07%), Query Frame = 0


Query: 13  LMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNELAKKLWRTL 72
           +MEEEGRFE EVAEVQAWWNSERFKLTRRPY+A+DVV+LRGSL+QSY SNE+AKKLW TL
Sbjct: 11  IMEEEGRFETEVAEVQAWWNSERFKLTRRPYSARDVVALRGSLKQSYGSNEMAKKLWTTL 70

Query: 73  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 132
           KTHQANGTASRTFGALDPVQVTMMAKHLD+IYVSGWQCSSTHT+TNEPGPDLADYPYDTV
Sbjct: 71  KTHQANGTASRTFGALDPVQVTMMAKHLDSIYVSGWQCSSTHTTTNEPGPDLADYPYDTV 130

Query: 133 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 192
           PNKVEHLFFAQQYHDRKQREARMSMSREERA+TPY+DYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC 190

Query: 193 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 252
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 250

Query: 253 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGKPGAELQALEDQWIS 312
           TDAVAATLIQTNVD RDHQFILGATNPNLRGKSLA +LAE MAAGK G +LQA+ED W++
Sbjct: 251 TDAVAATLIQTNVDTRDHQFILGATNPNLRGKSLANMLAEGMAAGKNGPQLQAIEDNWLA 310

Query: 313 MAQLKTFSECVTDAIMNTNATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 372
           +AQLKTFSECV DAI + N TE EKRR+++EWMNHSSY+KC+SNEQ REIAE+LGL+NLF
Sbjct: 311 IAQLKTFSECVMDAIKSMNITEDEKRRRMNEWMNHSSYDKCLSNEQAREIAERLGLQNLF 370

Query: 373 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSTHP 432
           WDWDLPRTREGFYRF+GSVMAAIVRGWAFAPHADLIWMETSSPD+VECT FA+GVKS HP
Sbjct: 371 WDWDLPRTREGFYRFRGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTRFAEGVKSMHP 430

Query: 433 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVIDTFARDY 492
           E MLAYNLSPSFNWDASGM+D+ M +FIPRIA+LGFCWQFITLAGFHADALV DTFARD+
Sbjct: 431 EIMLAYNLSPSFNWDASGMTDEHMRDFIPRIAKLGFCWQFITLAGFHADALVTDTFARDF 490

Query: 493 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 548
           ARRGMLAYVE+IQREER+NGVDTLAHQKWSGAN+YDRYLKTVQGGISSTAAMGKG
Sbjct: 491 ARRGMLAYVEKIQREERNNGVDTLAHQKWSGANFYDRYLKTVQGGISSTAAMGKG 545

[Close]

BLAST of Lsi10G001460 vs. ExPASy Swiss-Prot
Match: P15479 (Isocitrate lyase OS=Ricinus communis OX=3988 PE=2 SV=1)

HSP 1 Score: 988.0 bits (2553), Expect = 4.4e-287
Identity = 475/535 (88.79%), Postives = 507/535 (94.77%), Query Frame = 0


Query: 13  LMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNELAKKLWRTL 72
           +MEEEGRFEAEVAEVQAWWNSERFKLTRRPYTA+DVV+LRG+L+QSYASNELAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTARDVVALRGNLKQSYASNELAKKLWRTL 70

Query: 73  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 132
           KTHQANGTASRTFGALDPVQVTMMAKHLD+IYVSGWQCSSTHT+TNEPGPDLADYPYDTV
Sbjct: 71  KTHQANGTASRTFGALDPVQVTMMAKHLDSIYVSGWQCSSTHTTTNEPGPDLADYPYDTV 130

Query: 133 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 192
           PNKVEHLFFAQQYHDRKQREARMSMSREERA+TPY+DYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERARTPYVDYLKPIIADGDTGFGGTTATVKLC 190

Query: 193 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 252
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVA+SEHINRLVAARLQFDVMGVET+LVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAISEHINRLVAARLQFDVMGVETLLVAR 250

Query: 253 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGKPGAELQALEDQWIS 312
           TDA AA LIQ+NVD RDHQFILG TNPNLRGKSLA +LA  MA GK GAELQA ED W++
Sbjct: 251 TDAEAANLIQSNVDTRDHQFILGVTNPNLRGKSLATLLATGMANGKTGAELQATEDNWLA 310

Query: 313 MAQLKTFSECVTDAIMNTNATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 372
           MAQLKTF ECV DAI N NA E EKRR+++EWMNH+SY+KC+S EQGREIA+++GLKNLF
Sbjct: 311 MAQLKTFPECVMDAIKNMNAGEDEKRRRMNEWMNHTSYDKCLSYEQGREIADRMGLKNLF 370

Query: 373 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSTHP 432
           WDWDLPRTREGFYRFKGSVMAA+VRG AFAPHAD+IWMET+ PD  ECT FA+GVKS HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAVVRGRAFAPHADIIWMETAKPDFAECTAFAEGVKSMHP 430

Query: 433 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVIDTFARDY 492
           E MLAYNLSPSFNWDASGM+D+QM +FIPRIARLGFCWQFITL GFHADALVIDTFA+DY
Sbjct: 431 EIMLAYNLSPSFNWDASGMTDEQMRDFIPRIARLGFCWQFITLGGFHADALVIDTFAKDY 490

Query: 493 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 548
           ARRGMLAYVERIQREER NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG
Sbjct: 491 ARRGMLAYVERIQREERKNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 545

[Close]

BLAST of Lsi10G001460 vs. ExPASy Swiss-Prot
Match: P49297 (Isocitrate lyase OS=Solanum lycopersicum OX=4081 PE=2 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 2.0e-279
Identity = 463/545 (84.95%), Postives = 507/545 (93.03%), Query Frame = 0


Query: 13  LMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNELAKKLWRTL 72
           +MEEE RFEAEVAEVQAWWN+ERF+LT+R Y+A+DVV+LRG++RQSYASNELA+KLWRTL
Sbjct: 11  IMEEERRFEAEVAEVQAWWNTERFRLTKRAYSARDVVALRGTMRQSYASNELAQKLWRTL 70

Query: 73  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 132
           KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 133 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 192
           PNKVEHLFFAQQYHDRKQREARMSM REERA+TP+IDYLKPIIADGDTGFGG TATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMCREERARTPFIDYLKPIIADGDTGFGGATATVKLC 190

Query: 193 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 252
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMG ETVLVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGTETVLVAR 250

Query: 253 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGKPGAELQALEDQWIS 312
           TDAVAATLIQTNVD RDHQFILGA+NPNL+GK LA  L+EAMAAGK G ELQA+ED W+ 
Sbjct: 251 TDAVAATLIQTNVDTRDHQFILGASNPNLKGKGLATHLSEAMAAGKTGPELQAIEDNWLG 310

Query: 313 MAQLKTFSECVTDAIMNTNATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 372
           MA+LKTFS+CVTDAI   N  E EK+RKL+EWMNHSSYEKC+S+EQ RE+AE+LGL NLF
Sbjct: 311 MAELKTFSQCVTDAIKKMNLAEYEKQRKLNEWMNHSSYEKCLSHEQAREVAERLGLPNLF 370

Query: 373 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSTHP 432
           WDWDLPRTREGFYRF+GSV AAIVRGWAFA + DL+WMETSSPD+VECT F++GVK+  P
Sbjct: 371 WDWDLPRTREGFYRFQGSVEAAIVRGWAFAEYCDLVWMETSSPDMVECTKFSQGVKTLRP 430

Query: 433 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVIDTFARDY 492
           E MLAYNLSPSFNWDASGM+D QM +FIPRIA+LG+CWQFITLAGFHADAL++DTFA+D+
Sbjct: 431 ELMLAYNLSPSFNWDASGMNDNQMMDFIPRIAKLGYCWQFITLAGFHADALIVDTFAKDF 490

Query: 493 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG---KQ 552
           ARRGMLAYVE+IQREERSNGVDTLAHQKWSGANYYDR L+TVQGGI+STAAMGKG   +Q
Sbjct: 491 ARRGMLAYVEKIQREERSNGVDTLAHQKWSGANYYDRVLRTVQGGITSTAAMGKGVTEEQ 550

Query: 553 IHHYW 555
               W
Sbjct: 551 FEEKW 555

[Close]

BLAST of Lsi10G001460 vs. ExPASy TrEMBL
Match: A0A1S3C1U5 (Isocitrate lyase OS=Cucumis melo OX=3656 GN=LOC103495946 PE=3 SV=1)

HSP 1 Score: 1070.8 bits (2768), Expect = 1.9e-309
Identity = 526/535 (98.32%), Postives = 532/535 (99.44%), Query Frame = 0


Query: 13  LMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNELAKKLWRTL 72
           +MEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 73  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 132
           KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 133 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 192
           PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 190

Query: 193 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 252
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVET+LVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETILVAR 250

Query: 253 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGKPGAELQALEDQWIS 312
           TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGK GAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 310

Query: 313 MAQLKTFSECVTDAIMNTNATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 372
           MAQLKTFSECVTDAIMNT ATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNTIATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 373 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSTHP 432
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKS HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSIHP 430

Query: 433 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVIDTFARDY 492
           ETMLAYNLSPSFNWDASGMSDK+MEEFIPRIARLGFCWQFITLAGFHADALV+DTFARDY
Sbjct: 431 ETMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 490

Query: 493 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 548
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 545

[Close]

BLAST of Lsi10G001460 vs. ExPASy TrEMBL
Match: A0A0A0LR30 (Isocitrate lyase OS=Cucumis sativus OX=3659 GN=Csa_2G420990 PE=3 SV=1)

HSP 1 Score: 1070.1 bits (2766), Expect = 3.2e-309
Identity = 524/535 (97.94%), Postives = 531/535 (99.25%), Query Frame = 0


Query: 13  LMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNELAKKLWRTL 72
           +MEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 73  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 132
           KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 133 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 192
           PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 190

Query: 193 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 252
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLV VSEHINRLVAARLQFDVMGVET+LVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVGVSEHINRLVAARLQFDVMGVETILVAR 250

Query: 253 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGKPGAELQALEDQWIS 312
           TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAG LAEAMAAGK GAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGALAEAMAAGKTGAELQALEDQWIS 310

Query: 313 MAQLKTFSECVTDAIMNTNATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 372
           MAQLKTFSECVTDAIMNTNATE+EKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 373 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSTHP 432
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKG+KS HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGMKSIHP 430

Query: 433 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVIDTFARDY 492
           ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALV+DTFARDY
Sbjct: 431 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 490

Query: 493 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 548
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 545

[Close]

BLAST of Lsi10G001460 vs. ExPASy TrEMBL
Match: A0A6J1JFM0 (Isocitrate lyase OS=Cucurbita maxima OX=3661 GN=LOC111484071 PE=3 SV=1)

HSP 1 Score: 1062.8 bits (2747), Expect = 5.2e-307
Identity = 522/535 (97.57%), Postives = 528/535 (98.69%), Query Frame = 0


Query: 13  LMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNELAKKLWRTL 72
           +MEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 73  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 132
           KTHQAN TASRTFGALDPVQVTMMAKHLD+IYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANSTASRTFGALDPVQVTMMAKHLDSIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 133 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 192
           PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPY+DYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGFGGTTATVKLC 190

Query: 193 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 252
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 250

Query: 253 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGKPGAELQALEDQWIS 312
           TDAVAATLIQTNVD RDHQFILGATNPNLRGKSLAGVLAEAMAAGK GAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDSRDHQFILGATNPNLRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 310

Query: 313 MAQLKTFSECVTDAIMNTNATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 372
           MAQLKTFSECVTDAIMN+N TESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNSNGTESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 373 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSTHP 432
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKS HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSVHP 430

Query: 433 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVIDTFARDY 492
           E MLAYNLSPSFNWDASGMSDKQMEEFIP IARLGFCWQFITLAGFHADALVIDTFARDY
Sbjct: 431 EIMLAYNLSPSFNWDASGMSDKQMEEFIPTIARLGFCWQFITLAGFHADALVIDTFARDY 490

Query: 493 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 548
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 545

[Close]

BLAST of Lsi10G001460 vs. ExPASy TrEMBL
Match: A0A6J1FXZ6 (Isocitrate lyase OS=Cucurbita moschata OX=3662 GN=LOC111448305 PE=3 SV=1)

HSP 1 Score: 1062.0 bits (2745), Expect = 8.8e-307
Identity = 522/535 (97.57%), Postives = 528/535 (98.69%), Query Frame = 0


Query: 13  LMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNELAKKLWRTL 72
           +MEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 73  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 132
           KTHQAN TASRTFGALDPVQVTMMAKHLD+IYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANSTASRTFGALDPVQVTMMAKHLDSIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 133 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 192
           PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPY+DYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGFGGTTATVKLC 190

Query: 193 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 252
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 250

Query: 253 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGKPGAELQALEDQWIS 312
           TDAVAATLIQTNVD RDHQFILGATNPNLRGKSLAGVLAEAMAAGK GAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDGRDHQFILGATNPNLRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 310

Query: 313 MAQLKTFSECVTDAIMNTNATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 372
           MAQLKTFSECVTDAIMN+N TESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNSNGTESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 373 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSTHP 432
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKS HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSVHP 430

Query: 433 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVIDTFARDY 492
           E MLAYNLSPSFNWDASGMSDKQMEEFIP IARLGFCWQFITLAGFHADALVIDTFARDY
Sbjct: 431 EIMLAYNLSPSFNWDASGMSDKQMEEFIPTIARLGFCWQFITLAGFHADALVIDTFARDY 490

Query: 493 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 548
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 545

[Close]

BLAST of Lsi10G001460 vs. ExPASy TrEMBL
Match: A0A6J1I0N5 (Isocitrate lyase OS=Cucurbita maxima OX=3661 GN=LOC111469780 PE=3 SV=1)

HSP 1 Score: 1048.5 bits (2710), Expect = 1.0e-302
Identity = 512/535 (95.70%), Postives = 524/535 (97.94%), Query Frame = 0


Query: 13  LMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNELAKKLWRTL 72
           +MEEEGRFEAEVAEV AWWNSERFKLTRRPY+AKDVVSLRGSLRQSYASNELAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVHAWWNSERFKLTRRPYSAKDVVSLRGSLRQSYASNELAKKLWRTL 70

Query: 73  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 132
           KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 133 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 192
           PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPY+DYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGFGGTTATVKLC 190

Query: 193 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 252
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 250

Query: 253 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGKPGAELQALEDQWIS 312
           TDAV ATLIQTNVD RDHQFILGATNPNLRGKSLAG L+EAMAAGK GAELQALEDQWIS
Sbjct: 251 TDAVGATLIQTNVDSRDHQFILGATNPNLRGKSLAGALSEAMAAGKTGAELQALEDQWIS 310

Query: 313 MAQLKTFSECVTDAIMNTNATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 372
           MAQLKTFSECVTDAIMN NATE+EKRRKLDEWM+ SSYEKC+SNEQGREI EKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNMNATENEKRRKLDEWMSQSSYEKCLSNEQGREIVEKLGLKNLF 370

Query: 373 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSTHP 432
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPD+VECT+FAKGVKS HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDMVECTSFAKGVKSVHP 430

Query: 433 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVIDTFARDY 492
           E MLAYNLSPSFNWDASGMSDK+MEEFIPRIARLGFCWQFITLAGFHADALVIDTFARDY
Sbjct: 431 ELMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADALVIDTFARDY 490

Query: 493 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 548
           ARRGML YVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMG+G
Sbjct: 491 ARRGMLGYVERIQREERANGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGRG 545

[Close]

BLAST of Lsi10G001460 vs. NCBI nr
Match: XP_038900496.1 (isocitrate lyase [Benincasa hispida])

HSP 1 Score: 1071.6 bits (2770), Expect = 2.2e-309
Identity = 525/535 (98.13%), Postives = 531/535 (99.25%), Query Frame = 0


Query: 13  LMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNELAKKLWRTL 72
           +MEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 73  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 132
           K HQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KNHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 133 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 192
           PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPY+DYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGFGGTTATVKLC 190

Query: 193 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 252
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVET+LVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETILVAR 250

Query: 253 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGKPGAELQALEDQWIS 312
           TDAVAATLIQTNVD RDHQFILGATNPNLRGKSLAGVLAEAMAAGK GAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDSRDHQFILGATNPNLRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 310

Query: 313 MAQLKTFSECVTDAIMNTNATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 372
           MAQLKTFSECVTDAIMNTNATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNTNATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 373 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSTHP 432
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKS HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSVHP 430

Query: 433 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVIDTFARDY 492
           ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALV+DTFARDY
Sbjct: 431 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 490

Query: 493 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 548
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 545

[Close]

BLAST of Lsi10G001460 vs. NCBI nr
Match: P49296.1 (RecName: Full=Isocitrate lyase; Short=ICL; AltName: Full=Isocitrase; AltName: Full=Isocitratsysase [Cucumis sativus] >CAA84632.1 isocitrate lyase [Cucumis sativus])

HSP 1 Score: 1071.6 bits (2770), Expect = 2.2e-309
Identity = 525/535 (98.13%), Postives = 532/535 (99.44%), Query Frame = 0


Query: 13  LMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNELAKKLWRTL 72
           +MEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 73  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 132
           KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 133 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 192
           PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 190

Query: 193 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 252
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVET+LVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETILVAR 250

Query: 253 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGKPGAELQALEDQWIS 312
           TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAG LAEAMAAGK GAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGALAEAMAAGKTGAELQALEDQWIS 310

Query: 313 MAQLKTFSECVTDAIMNTNATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 372
           MAQLKTFSECVTDAIMNTNATE+EKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 373 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSTHP 432
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKG+KS HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGMKSIHP 430

Query: 433 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVIDTFARDY 492
           ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALV+DTFARDY
Sbjct: 431 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 490

Query: 493 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 548
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 545

[Close]

BLAST of Lsi10G001460 vs. NCBI nr
Match: XP_008455845.1 (PREDICTED: isocitrate lyase [Cucumis melo])

HSP 1 Score: 1070.8 bits (2768), Expect = 3.9e-309
Identity = 526/535 (98.32%), Postives = 532/535 (99.44%), Query Frame = 0


Query: 13  LMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNELAKKLWRTL 72
           +MEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 73  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 132
           KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 133 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 192
           PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 190

Query: 193 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 252
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVET+LVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETILVAR 250

Query: 253 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGKPGAELQALEDQWIS 312
           TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGK GAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 310

Query: 313 MAQLKTFSECVTDAIMNTNATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 372
           MAQLKTFSECVTDAIMNT ATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNTIATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 373 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSTHP 432
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKS HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSIHP 430

Query: 433 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVIDTFARDY 492
           ETMLAYNLSPSFNWDASGMSDK+MEEFIPRIARLGFCWQFITLAGFHADALV+DTFARDY
Sbjct: 431 ETMLAYNLSPSFNWDASGMSDKEMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 490

Query: 493 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 548
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 545

[Close]

BLAST of Lsi10G001460 vs. NCBI nr
Match: XP_004151880.1 (isocitrate lyase [Cucumis sativus] >KGN63267.1 hypothetical protein Csa_022060 [Cucumis sativus])

HSP 1 Score: 1070.1 bits (2766), Expect = 6.6e-309
Identity = 524/535 (97.94%), Postives = 531/535 (99.25%), Query Frame = 0


Query: 13  LMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNELAKKLWRTL 72
           +MEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 73  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 132
           KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 133 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 192
           PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 190

Query: 193 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 252
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLV VSEHINRLVAARLQFDVMGVET+LVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVGVSEHINRLVAARLQFDVMGVETILVAR 250

Query: 253 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGKPGAELQALEDQWIS 312
           TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAG LAEAMAAGK GAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGALAEAMAAGKTGAELQALEDQWIS 310

Query: 313 MAQLKTFSECVTDAIMNTNATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 372
           MAQLKTFSECVTDAIMNTNATE+EKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNTNATENEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 373 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSTHP 432
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKG+KS HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGMKSIHP 430

Query: 433 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVIDTFARDY 492
           ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALV+DTFARDY
Sbjct: 431 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVVDTFARDY 490

Query: 493 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 548
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 545

[Close]

BLAST of Lsi10G001460 vs. NCBI nr
Match: KAG7010723.1 (hypothetical protein SDJN02_27519, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1067.0 bits (2758), Expect = 5.6e-308
Identity = 524/537 (97.58%), Postives = 530/537 (98.70%), Query Frame = 0


Query: 13  LMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNELAKKLWRTL 72
           +MEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASN+LAKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNDLAKKLWRTL 70

Query: 73  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 132
           KTHQAN TASRTFGALDPVQVTMMAKHLD+IYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KTHQANSTASRTFGALDPVQVTMMAKHLDSIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 133 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 192
           PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPY+DYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYVDYLKPIIADGDTGFGGTTATVKLC 190

Query: 193 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 252
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 250

Query: 253 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGKPGAELQALEDQWIS 312
           TDAVAATLIQTNVD RDHQFILGATNPNLRGKSLAGVLAEAMAAGK GAELQALEDQWIS
Sbjct: 251 TDAVAATLIQTNVDSRDHQFILGATNPNLRGKSLAGVLAEAMAAGKTGAELQALEDQWIS 310

Query: 313 MAQLKTFSECVTDAIMNTNATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 372
           MAQLKTFSECVTDAIMN+N TESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF
Sbjct: 311 MAQLKTFSECVTDAIMNSNGTESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 370

Query: 373 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSTHP 432
           WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKS HP
Sbjct: 371 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSVHP 430

Query: 433 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVIDTFARDY 492
           E MLAYNLSPSFNWDASGMSDKQMEEFIP IARLGFCWQFITLAGFHADALVIDTFARDY
Sbjct: 431 EIMLAYNLSPSFNWDASGMSDKQMEEFIPTIARLGFCWQFITLAGFHADALVIDTFARDY 490

Query: 493 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGKQ 550
           ARRGMLAYVERIQREER+NGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGKQ
Sbjct: 491 ARRGMLAYVERIQREERNNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKGKQ 547

[Close]

BLAST of Lsi10G001460 vs. TAIR 10
Match: AT3G21720.1 (isocitrate lyase )

HSP 1 Score: 944.9 bits (2441), Expect = 3.0e-275
Identity = 454/535 (84.86%), Postives = 493/535 (92.15%), Query Frame = 0


Query: 13  LMEEEGRFEAEVAEVQAWWNSERFKLTRRPYTAKDVVSLRGSLRQSYASNELAKKLWRTL 72
           +MEEEGRFEAEVAEVQ WW+SERFKLTRRPYTA+DVV+LRG L+Q YASNE+AKKLWRTL
Sbjct: 11  IMEEEGRFEAEVAEVQTWWSSERFKLTRRPYTARDVVALRGHLKQGYASNEMAKKLWRTL 70

Query: 73  KTHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 132
           K+HQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV
Sbjct: 71  KSHQANGTASRTFGALDPVQVTMMAKHLDTIYVSGWQCSSTHTSTNEPGPDLADYPYDTV 130

Query: 133 PNKVEHLFFAQQYHDRKQREARMSMSREERAKTPYIDYLKPIIADGDTGFGGTTATVKLC 192
           PNKVEHLFFAQQYHDRKQREARMSMSREER KTP++DYLKPIIADGDTGFGGTTATVKLC
Sbjct: 131 PNKVEHLFFAQQYHDRKQREARMSMSREERTKTPFVDYLKPIIADGDTGFGGTTATVKLC 190

Query: 193 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGVETVLVAR 252
           KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMG ETVLVAR
Sbjct: 191 KLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRLVAARLQFDVMGTETVLVAR 250

Query: 253 TDAVAATLIQTNVDKRDHQFILGATNPNLRGKSLAGVLAEAMAAGKPGAELQALEDQWIS 312
           TDAVAATLIQ+N+D RDHQFILGATNP+LRGKSL+ +LAE M  GK G  LQ++EDQW+ 
Sbjct: 251 TDAVAATLIQSNIDARDHQFILGATNPSLRGKSLSSLLAEGMTVGKNGPALQSIEDQWLG 310

Query: 313 MAQLKTFSECVTDAIMNTNATESEKRRKLDEWMNHSSYEKCISNEQGREIAEKLGLKNLF 372
            A L TFSE V  AI   N  E+EK ++L EW+ H+ YE C+SNEQGR +A KLG+ +LF
Sbjct: 311 SAGLMTFSEAVVQAIKRMNLNENEKNQRLSEWLTHARYENCLSNEQGRVLAAKLGVTDLF 370

Query: 373 WDWDLPRTREGFYRFKGSVMAAIVRGWAFAPHADLIWMETSSPDLVECTTFAKGVKSTHP 432
           WDWDLPRTREGFYRF+GSV AA+VRGWAFA  AD+IWMET+SPDL ECT FA+G+KS  P
Sbjct: 371 WDWDLPRTREGFYRFQGSVAAAVVRGWAFAQIADIIWMETASPDLNECTQFAEGIKSKTP 430

Query: 433 ETMLAYNLSPSFNWDASGMSDKQMEEFIPRIARLGFCWQFITLAGFHADALVIDTFARDY 492
           E MLAYNLSPSFNWDASGM+D+QM EFIPRIARLG+CWQFITLAGFHADALV+DTFA+DY
Sbjct: 431 EVMLAYNLSPSFNWDASGMTDQQMVEFIPRIARLGYCWQFITLAGFHADALVVDTFAKDY 490

Query: 493 ARRGMLAYVERIQREERSNGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 548
           ARRGMLAYVERIQREER++GVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG
Sbjct: 491 ARRGMLAYVERIQREERTHGVDTLAHQKWSGANYYDRYLKTVQGGISSTAAMGKG 545

[Close]

BLAST of Lsi10G001460 vs. TAIR 10
Match: AT1G21440.1 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 62.0 bits (149), Expect = 1.8e-09
Identity = 37/86 (43.02%), Postives = 50/86 (58.14%), Query Frame = 0


Query: 173 PIIADGDTGFGGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINR 232
           PIIAD DTG G      +  K  +  GAAG  +EDQ +  K+CGHM GK ++   EH  +
Sbjct: 115 PIIADADTGGGNALNVQRTVKDLIAAGAAGCFLEDQ-AWPKRCGHMRGKEVIPAEEHAAK 174

Query: 233 LVAARLQFDVMG-VETVLVARTDAVA 258
           + +AR   D +G  +  L+ARTDA A
Sbjct: 175 IASAR---DAIGDADFFLIARTDARA 196

[Close]

BLAST of Lsi10G001460 vs. TAIR 10
Match: AT1G77060.1 (Phosphoenolpyruvate carboxylase family protein )

HSP 1 Score: 61.2 bits (147), Expect = 3.0e-09
Identity = 38/88 (43.18%), Postives = 50/88 (56.82%), Query Frame = 0


Query: 173 PIIADGDTGFGGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINR 232
           PIIAD DTG G      +  K  +  GAAG  +EDQ +  KKCGHM GK ++   EH  +
Sbjct: 117 PIIADADTGGGNALNIQRTVKDLIAAGAAGCFLEDQ-AWPKKCGHMRGKQVIPAEEHAAK 176

Query: 233 LVAARLQFDVMG-VETVLVARTDAVAAT 260
           + +AR   D +G  +  LVARTD  A +
Sbjct: 177 IASAR---DAIGDSDFFLVARTDVRATS 200

The following BLAST results are available for this feature:

BLAST of Lsi10G001460 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P49296	2.9e-312	98.13	Isocitrate lyase OS=Cucumis sativus OX=3659 PE=3 SV=1	[more]
P93110	1.4e-309	97.57	Isocitrate lyase OS=Cucurbita maxima OX=3661 PE=2 SV=1	[more]
P17069	2.5e-290	89.53	Isocitrate lyase OS=Gossypium hirsutum OX=3635 PE=2 SV=1	[more]
P15479	4.4e-287	88.79	Isocitrate lyase OS=Ricinus communis OX=3988 PE=2 SV=1	[more]
P49297	2.0e-279	84.95	Isocitrate lyase OS=Solanum lycopersicum OX=4081 PE=2 SV=1	[more]

BLAST of Lsi10G001460 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C1U5	1.9e-309	98.32	Isocitrate lyase OS=Cucumis melo OX=3656 GN=LOC103495946 PE=3 SV=1	[more]
A0A0A0LR30	3.2e-309	97.94	Isocitrate lyase OS=Cucumis sativus OX=3659 GN=Csa_2G420990 PE=3 SV=1	[more]
A0A6J1JFM0	5.2e-307	97.57	Isocitrate lyase OS=Cucurbita maxima OX=3661 GN=LOC111484071 PE=3 SV=1	[more]
A0A6J1FXZ6	8.8e-307	97.57	Isocitrate lyase OS=Cucurbita moschata OX=3662 GN=LOC111448305 PE=3 SV=1	[more]
A0A6J1I0N5	1.0e-302	95.70	Isocitrate lyase OS=Cucurbita maxima OX=3661 GN=LOC111469780 PE=3 SV=1	[more]

BLAST of Lsi10G001460 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038900496.1	2.2e-309	98.13	isocitrate lyase [Benincasa hispida]	[more]
P49296.1	2.2e-309	98.13	RecName: Full=Isocitrate lyase; Short=ICL; AltName: Full=Isocitrase; AltName: Fu...	[more]
XP_008455845.1	3.9e-309	98.32	PREDICTED: isocitrate lyase [Cucumis melo]	[more]
XP_004151880.1	6.6e-309	97.94	isocitrate lyase [Cucumis sativus] >KGN63267.1 hypothetical protein Csa_022060 [...	[more]
KAG7010723.1	5.6e-308	97.58	hypothetical protein SDJN02_27519, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of Lsi10G001460 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G21720.1	3.0e-275	84.86	isocitrate lyase	[more]
AT1G21440.1	1.8e-09	43.02	Phosphoenolpyruvate carboxylase family protein	[more]
AT1G77060.1	3.0e-09	43.18	Phosphoenolpyruvate carboxylase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (USVL1VR-Ls) v1
Date Performed: 2021-10-18

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR040442	Pyruvate kinase-like domain superfamily	GENE3D	3.20.20.60		coord: 19..537 e-value: 1.2E-244 score: 814.4
IPR006254	Isocitrate lyase	PFAM	PF00463	ICL	coord: 23..548 e-value: 1.4E-272 score: 904.9
IPR006254	Isocitrate lyase	PIRSF	PIRSF001362	ICL	coord: 14..341 e-value: 3.0E-64 score: 214.5 coord: 370..553 e-value: 1.5E-52 score: 175.9
IPR006254	Isocitrate lyase	TIGRFAM	TIGR01346	TIGR01346	coord: 24..548 e-value: 4.9E-263 score: 871.3
IPR006254	Isocitrate lyase	PANTHER	PTHR21631	ISOCITRATE LYASE/MALATE SYNTHASE	coord: 14..552
None	No IPR available	GENE3D	1.10.10.850		coord: 264..379 e-value: 1.2E-244 score: 814.4
IPR018523	Isocitrate lyase/phosphorylmutase, conserved site	PROSITE	PS00161	ISOCITRATE_LYASE	coord: 213..218
IPR039556	ICL/PEPM domain	CDD	cd00377	ICL_PEPM	coord: 77..491 e-value: 2.30645E-46 score: 161.117
IPR015813	Pyruvate/Phosphoenolpyruvate kinase-like domain superfamily	SUPERFAMILY	51621	Phosphoenolpyruvate/pyruvate domain	coord: 13..537

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lsi10G001460.1	Lsi10G001460.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006097	glyoxylate cycle
biological_process	GO:0006099	tricarboxylic acid cycle
biological_process	GO:0019752	carboxylic acid metabolic process
cellular_component	GO:0009514	glyoxysome
molecular_function	GO:0004451	isocitrate lyase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003824	catalytic activity